Log In Sign up
The largest database of trusted experimental protocols
> Disorders > Neoplastic Process > Chordoma

Chordoma

Chordoma is a rare, slow-growing type of tumor that typically occurs in the base of the skull or the spine.
These tumors arise from remnants of the notochord, a structure present during embryonic development that eventually becomes the vertebral column.
Chordomas can be locally aggressive and destructive, but they rarely spread to other parts of the body.
Symptoms may include pain, neurological deficits, and swelling.
Early diagnosis and appropriate treatment, which may involve surgery, radiation therapy, or targeted therapies, are important for managing this condition.
Further research is needed to improve understanding of the underlying biology and optimize treatment approaches for chordoma patients.

Most cited protocols related to «Chordoma»

1
Establishing Chordoma Cell Line Collection
Cited 7 times
MEDLINE was searched for all references to chordoma cell lines using the search terms “chordoma cell line,” “chordoma cell culture,” and “chordoma AND in vitro,” and each resulting article was read to determine whether it described or reported use of a chordoma cell line. The corresponding authors of these publications were contacted, and a sample of each of their chordoma cell lines was requested, whether published or unpublished. Additionally, other unpublished chordoma cell lines were solicited from attendees of the First (2007) and Second (2008) International Chordoma Workshops and through personal communication (JS) with researchers who have published on chordoma and clinicians at medical centers with a high volume of chordoma cases.
Once received, cells were grown to 90% confluence in 75 cm2 tissue culture flasks and passaged according to methods described in their respective publications or as communicated by the creators of the cell lines. The culture media, serum, and substrate used for each cell line are indicated in Table 1. All other lines were grown and passaged using methods and medias as recommended by the providers. Primary human cultures consisted of middle passage MRC-5 (American Type Tissue Culture) derived from human embryonic lung or NHF1, a gift from M. Cordeiro-S`tone, derived from newborn foreskin.
Brüderlein S., Sommer J.B., Meltzer P.S., Li S., Osada T., Ng D., Möller P., Alcorta D.A, & Kelley M.J. (2010). Molecular Characterization of Putative Chordoma Cell Lines. Sarcoma, 2010, 630129.
Full text: Click here
Publication 2010
Cell Culture Techniques Cell Lines Cells Chordoma Culture Media Embryo Foreskin Homo sapiens Infant, Newborn Lung Serum Tissues Workshops
2
Molecular Profiling of Myoepithelial Tumors
Cited 7 times
Seventy-one myoepithelial tumors with available tissue for molecular analysis were retrieved from the surgical pathology and consultation files of the authors. Slides were re-reviewed in corroboration with the immunohistochemical panel in all cases. Minimum criteria for confirming the morphologic diagnosis included the co-reactivity for EMA +/− cytokeratin and S100+/− GFAP. Five cases were excluded as failing FISH analysis: four due to decalcification and one due to hybridization failure.
The tumors were assessed morphologically for pattern of growth, duct formation, clear cell component, epithelioid versus spindle cell composition, nuclear pleomorphism, mitotic activity, and necrosis. The tumor location was recorded, including the anatomic structures involved, and lesions were subclassified into three subgroups, cutaneous, superficial (subcutaneous), and deep (below the fascial plane).
In order to investigate a potential relationship between soft tissue ME tumors and their salivary gland counterpart, we included five salivary gland ME carcinomas (ex-pleomorphic adenoma) for the genetic analysis. A group of five salivary mucoepidermoid carcinomas and five cutaneous eccrine hidradenomas were also tested, as recent evidence suggested similar genetic abnormalities (see Discussion section). As chordoma periphericum has been considered a potentially related tumor, we have included two such examples occurring in the bone, confirmed based on their immunopositivity for t-Brachyury and a similar microscopic appearance with their axial counterpart (Nielsen et al., 2001 (link); Scolyer et al., 2004 (link); Tirabosco et al., 2008 (link)). In addition, three ossifying fibromyxoid tumors (OFT) were included in the analysis, as this tumor often shares morphologic and immunophenotypic features with ME tumors.
Antonescu C.R., Zhang L., Chang N.E., Pawel B.R., Travis W., Katabi N., Edelman M., Rosenberg A.E., Nielsen G.P., Cin P.D, & Fletcher C.D. (2010). EWSR1-POU5F1 Fusion in Soft Tissue Myoepithelial Tumors. A Molecular Analysis of 66 Cases, Including Soft Tissue, Bone and Visceral Lesions, Showing Common Involvement of the EWSR1 gene. Genes, chromosomes & cancer, 49(12), 1114-1124.
Publication 2010
Acrospiroma Bones Brachyury protein Carcinoma Cells Cellular Structures Childbirth Chordoma Congenital Abnormality Crossbreeding Cytokeratin Diagnosis Fascia Fishes Glial Fibrillary Acidic Protein Immunophenotyping Microscopy Mixed Salivary Gland Tumor Mucoepidermoid Carcinoma Myoepithelioma Myxofibroma Necrosis Neoplasms Neoplasms by Site S100 Proteins Salivary Glands Skin Tissues
3
Tissue Microarray Construction and Immunohistochemistry
Cited 6 times
Paraffin ‘donor’ blocks were selected for each case. Using a manual tissue arrayer (MTA-1, Beecher Instruments, (Sun Prairie, WI) 0.6 mm cores were transferred from each donor block to a blank recipient paraffin block and arrayed in triplicate by one of the authors (RRS). Use of decalcified blocks could not be avoided in 65% (66/102) cases. For chondroid chordomas, whenever possible, at least one core was obtained from chondroid predominant areas, and one core from more conventional appearing areas. The cores from the donor block were annealed to the recipient block by heating to 37°C for 5 minutes and gently pressing on a flat surface to make all cores level. Paraffin sections from the constructed tissue microarray block were cut (without tape transfer) and incubated with commercially available antibodies for S-100 protein, cytokeratin cocktail AE1/AE3, brachyury, EMA, CD24, podoplanin, ()GFAP, polyclonal ()CEA, and SOX-9. Antibodies, dilutions, company and pretreatment are summarized in Table 2. Staining was visualized using the ImmPRESS™ (Vector Labs, Burlingame, CA) detection system with 2 - diaminobenzidine (DAB) as the substrate chromogen.
Oakley G., Fuhrer K, & Seethala R. (2008). Brachyury, SOX-9, and Podoplanin, New Markers in the Skull Base Chordoma Vs Chondrosarcoma Differential: A Tissue Microarray Based Comparative Analysis. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 21(12), 1461-1469.
Publication 2008
Antibodies azo rubin S Brachyury protein Chordoma Cloning Vectors Cytokeratin Glial Fibrillary Acidic Protein Microarray Analysis Paraffin S100 Proteins Technique, Dilution Tissue Donors Tissues
4
Retrospective Brain Tumor Specimen Analysis
Cited 5 times
Formalin-fixed paraffin-embedded brain tumor resection specimens were obtained from the authors’ institutions. The cases of chordoid meningioma, chordoma and chordoid glioma were retrieved retrospectively, as well as collected prospectively. The rest of the cases were collected prospectively and date from 2014 to 2017. All meningioma cases were graded according to the 2016 WHO Classification of Tumors of the CNS [1 ]. The morphological meningioma variants were diagnosed if more than 50% of the morphologic pattern was present in the tumor [14 ]. The WHO grade II meningiomas in this study show either increased mitotic count (more or equal to 4 mitotic Figures per 10 high power fields (HPFs) by visual inspection), brain invasion, or are high risk variants (chordoid and clear cell). The other risk features of meningioma, e.g. necrosis, may or may not be present. All WHO grade III meningiomas in this study have more than 20 mitotic Figures per 10 random HPFs. The demographics include the age and gender of the patients and the location of the tumor for all cases. These studies were performed in compliance with the ethical guidelines of the Helsinki Declaration and approved by the ethical committees for research on human subjects of the authors’ institutions.
Georgescu M.M., Olar A., Mobley B.C., Faust P.L, & Raisanen J.M. (2018). Epithelial differentiation with microlumen formation in meningioma: diagnostic utility of NHERF1/EBP50 immunohistochemistry. Oncotarget, 9(47), 28652-28665.
Full text: Click here
Publication 2018
Brain Brain Neoplasms Cells Central Nervous System Neoplasms Chordoma Formalin Glioma Homo sapiens Meningioma Necrosis Neoplasms Neoplasms by Site Notochord Paraffin Patients
5
Flow Cytometry Analysis of Metastatic Solid Tumors
Cited 4 times
The flow cytometry analysis included 11 healthy donors under the age of 40, 15 healthy donors over the age of 40 and 30 patients with a variety of metastatic solid tumours over the age of 40. The median age of the healthy donor group < age 40 was 26 (range 18–31), with six males and five females. The median age of the healthy donor group > age 40 was 56 (range 46–78), with 12 males and three females. Peripheral blood mononuclear cells (PBMCs) from the healthy donors were obtained from the NIH Clinical Center Blood Bank (NCT00001846), as previously described. [8 (link)] The median age of the cancer patients was 64 (range 42–77), with 17 males and 13 females. The cancer patients all had metastatic solid tumours and were enrolled in a Phase I clinical trial (NCT01772004), with PBMCs that were examined in this study obtained prior to the initiation of therapy. The National Cancer Institute Institutional Review Board approved the trial procedures and informed consent was obtained in accordance with the Declaration of Helsinki. Patients had 13 different types of cancer including adrenocortical (n=2), breast (n=3), chordoma (n=1), gastrointestinal (GI) (n=6), lung (n=1), medullary thyroid (n=1), mesothelioma (n=3), neuroendocrine (n=1), ovarian (n=1), pancreatic (n=6), prostate (n=1), renal cell (n=3) and spindle cell (n=1) cancer, and the median number of prior anti-cancer therapies was 3 (range 1–15).
In an additional cohort of samples assessed by microarray, PBMCs were obtained from five healthy donors (median age: 54, three male, two female) from the NIH Clinical Center Blood Bank, as well as four patients with advanced GI cancer (median age: 56.5, two male, two female) enrolled in a Phase I study at the NCI (NCT00088413) [9 (link)]; PBMCs used in this study were isolated prior to treatment.
Lepone L.M., Donahue R.N., Grenga I., Metenou S., Richards J., Heery C.R., Madan R.A., Gulley J.L, & Schlom J. (2016). Analyses of 123 Peripheral Human Immune Cell Subsets: Defining Differences with Age and between Healthy Donors and Cancer Patients Not Detected in Analysis of Standard Immune Cell Types. Journal of Circulating Biomarkers, 5, 5.
Full text: Click here
Publication 2016
Age Groups Breast Cells Chordoma Donors Ethics Committees, Research Females Flow Cytometry Gastrointestinal Cancer Kidney Lung Males Malignant Neoplasms Medulla Oblongata Mesothelioma Microarray Analysis Neoplasm Metastasis Neurosecretory Systems Ovary Pancreas Patients PBMC Peripheral Blood Mononuclear Cells Prostate Thyroid Gland Tissue Donors Woman

Most recents protocols related to «Chordoma»

1
RNA and DNA Extraction from Chordoma Cells
Total RNA from chordoma cells (U-CH1 or MUG-Chor1) and tissues frozen in liquid nitrogen was isolated using the RNA extraction kit (Qiagen, Germantown, MD, USA, Cat# 74134) and TRIzol reagent. The DNA micro kit (Qiagen, Cat# 56304) was applied to isolate gDNA. The concentration of the extracted RNA was measured using NanoPhotometer Pearl (IMPLEN, Schatzbogen, Munchen, Germany, Model# 03384).
Lou J., Zhang H., Huang Q., Chen C., Wang W., Niu J., Xu J., Ren T., Huang Y., Tang X, & Guo W. (2023). The malignancy of chordomas is enhanced via a circTLK1/miR-16-5p/Smad3 positive feedback axis. Cell Death Discovery, 9, 64.
Full text: Click here
Publication 2023
Cells Chordoma Freezing Nitrogen Tissues trizol
2
Cell Proliferation Assay for Chordoma
Briefly, 100 μL of complete IMDM medium and 10 μL of CCK8 reagent (Dojindo Crop, Japan, Cat# CK04) were added to culture plates containing 8 × 103 cells/well. Data were collected over 4 days serially. Chordoma cells were analyzed using an EdU Kit (Beyotime Biotechnology, Beijing, China, Cat# C0075S) to confirm cell proliferation.
Lou J., Zhang H., Huang Q., Chen C., Wang W., Niu J., Xu J., Ren T., Huang Y., Tang X, & Guo W. (2023). The malignancy of chordomas is enhanced via a circTLK1/miR-16-5p/Smad3 positive feedback axis. Cell Death Discovery, 9, 64.
Full text: Click here
Publication 2023
Cell Proliferation Cells Chordoma Crop, Avian
3
Chordoma Cell Migration Assay
Transwell chambers (Corning, NY, USA, Cat# 3422) with an 8-μm pore size were used. Specially, 300 μL FBS-free IMDM medium containing 5 × 104 chordoma cells was added up to the top of the chamber. After culturing for 2 days, the following steps were referred to the relevant article [21 (link), 26 (link)]. Photographs were taken under the microscope (Leica Microsystems Inc), and migratory cells were counted as well as analyzed.
Lou J., Zhang H., Huang Q., Chen C., Wang W., Niu J., Xu J., Ren T., Huang Y., Tang X, & Guo W. (2023). The malignancy of chordomas is enhanced via a circTLK1/miR-16-5p/Smad3 positive feedback axis. Cell Death Discovery, 9, 64.
Full text: Click here
Publication 2023
Cells Chordoma Microscopy
4
Chordoma Cell Wound Healing Assay
Chordoma cells were prepared (1 × 106/well) overnight and an artificial wound was scratched. The photographs at the initial and three days were taken under a microscope (Leica Microsystems Inc, Bannockburn, IL, USA), then the width of the wound was measured and analyzed.
Lou J., Zhang H., Huang Q., Chen C., Wang W., Niu J., Xu J., Ren T., Huang Y., Tang X, & Guo W. (2023). The malignancy of chordomas is enhanced via a circTLK1/miR-16-5p/Smad3 positive feedback axis. Cell Death Discovery, 9, 64.
Full text: Click here
Publication 2023
Cells Chordoma Microscopy Wounds
5
Nuclear and Cytoplasmic RNA Extraction
The PARIS™ Kit (Thermo Scientific, Rockford, lL, USA, Cat# AM1921) was used to isolate nuclear and cytoplasmic components. An adequate number of chordoma cells (~106) were lysed in cell fractionation buffer for 5 min. After centrifugation for 3 min, the final nuclear and cytoplasmic RNA were separated and obtained.
Lou J., Zhang H., Huang Q., Chen C., Wang W., Niu J., Xu J., Ren T., Huang Y., Tang X, & Guo W. (2023). The malignancy of chordomas is enhanced via a circTLK1/miR-16-5p/Smad3 positive feedback axis. Cell Death Discovery, 9, 64.
Full text: Click here
Publication 2023
Buffers Cell Fractionation Centrifugation Chordoma Cytoplasm

Top products related to «Chordoma»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
U ch1 by American Type Culture Collection
Sourced in United States
The U-CH1 is a laboratory equipment designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cells.
3
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
U ch2 by American Type Culture Collection
Sourced in United States
The U-CH2 is a laboratory equipment product designed for cell culture applications. It serves as a compact and efficient incubator for maintaining optimal environmental conditions for cell growth and proliferation. The core function of the U-CH2 is to provide a controlled temperature, humidity, and gas atmosphere to support the cultivation of various cell types.
5
Mug chor1 by American Type Culture Collection
Sourced in United States
The MUG-Chor1 is a laboratory equipment designed for the efficient extraction and purification of DNA from various biological samples. It utilizes a magnetic separation technology to isolate and concentrate the DNA, allowing for accurate and reproducible results.
6
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
7
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
8
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
9
Rpmi 1640 medium by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Japan, France, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Israel, Sweden, Denmark, Macao, Brazil, Ireland, India, Austria, Netherlands, Holy See (Vatican City State), Poland, Norway, Cameroon, Hong Kong, Morocco, Singapore, Thailand, Argentina, Taiwan, Province of China, Palestine, State of, Finland, Colombia, United Arab Emirates
RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
10
L glutamine by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon
L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.

More about "Chordoma"

Chordomas, Notochord tumors, Skull base tumors, Spinal tumors, Locally aggressive tumors, Rare tumors, Slow-growing tumors, Neurological symptoms, Swelling, Surgical treatment, Radiation therapy, Targeted therapies, Underlying biology, Research optimization, PubCompare.ai, Protocols, Literature, Pre-prints, Patents, AI-powered comparisons, Reproducibility, Accuracy