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Anoxia

Anoxia: A condition characterized by a complete lack of oxygen supply to tissues, which can lead to severe tissue damage and organ failure.
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Most cited protocols related to «Anoxia»

Cultivation of E. coli and R. sphaeroides

The strains and plasmids used in this study are listed in Additional file 4: Table S3. E. coli strains were grown in Luria–Bertani medium at 37°C with shaking (180 rpm) or on solid growth medium, which contained 1.6% (w/v) agar. R. sphaeroides strains were cultivated at 32°C in 50-ml Erlenmeyer flasks containing 40 ml malate minimal medium (Additional file 4: Table S4) with continuous shaking at 140 rpm, resulting in a constant dissolved oxygen concentration of approximately 25–30 μM during the exponential phase. These growth conditions are designated as oxic growth. To achieve anoxic conditions, we used completely filled screw-cap Meplat bottles for liquid cultures, which were sealed with Parafilm and cultivated in the dark. The remaining oxygen was used up by the cultures within 60 seconds, as confirmed using an oxygen sensor. To allow anaerobic respiration, dimethyl sulphoxide (DMSO) was added as electron acceptor at a final concentration of 60 mM. Anoxic incubation over several days resulted in a final OD₆₆₀ of approximately 0.5. Conditions of iron limitation were achieved by transferring R. sphaeroides into iron-limited malate minimal medium containing the iron chelator 2,2′-dipyridyl (30 μM; Merck KGaA) three times. Inductively coupled plasma mass spectrometry (ICP-MS) using an Agilent 7500ce spectrometer confirmed that the iron content was drastically reduced in iron-limited medium (from 140 mg l^-1 to 16 mg l^-1) [22 (link)]. When required, antibiotics were added to liquid or solid growth media at the following concentrations: spectinomycin (10 μg ml^-1); kanamycin (25 μg ml^-1); tetracycline (2 μg ml^-1) (for R. sphaeroides); kanamycin (25 μg ml^-1); and tetracycline (20 μg ml^-1) (for E. coli).

Remes B., Berghoff B.A., Förstner K.U, & Klug G. (2014). Role of oxygen and the OxyR protein in the response to iron limitation in Rhodobacter sphaeroides. BMC Genomics, 15(1), 794.

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Publication 2014

Agar Anoxia Antibiotics, Antitubercular Chelating Agents Culture Media Escherichia coli Growth Disorders Iron Iron Metabolism Disorders Kanamycin malate Mass Spectrometry Neoplasm Metastasis Oxidants Oxygen Oxygen-25 Plasma Plasmids Respiration Spectinomycin Strains Sulfoxide, Dimethyl Tetracycline

Mitochondrial Respiration Modulation Protocols

For all respiration experiments, mitochondria were incubated in RM at 37°C in the presence of sodium and in the absence of exogenous substrates or energy phosphates in order to deplete endogenous Ca²⁺ from the mitochondrial matrix (5 (link), 6 (link), 9 (link)). After the depletion step, 10 mM Pi and 0.13 mM ADP were added, followed by glutamate (G, 10 mM) and malate (M, 1 mM) ± Ca²⁺. Addition of fuel (G+M) initiated a submaximal increase in J_o followed by a subsequent transition to State 4 J_o. For experiments designed to measure maximal J_o, State 3 was then elicited with a bolus of 1.3 mM ADP which resulted in consumption of all the oxygen in the chamber providing the fully reduced state (anoxia). Free Ca²⁺ concentrations were made using the calculator programs of Fabiato and Fabiato (26 (link)) translated to Labview VIs (National Instruments Corp, Austin, TX) by Reitz and Pollack (27 (link)).
Steady-state, intermediate J_o was attained using a progressive creatine kinase (CK) energetic clamp (28 (link)), we have recently modified (29 (link)). Briefly, by utilizing a large total creatine pool, excess CK, a known [ATP], and the CK equilibrium constant (K_CK, 150 (30 (link))), the extramitochondrial ATP/ADP ratio, and thus, free energy of ATP hydrolysis (ΔG_ATPe) can be calculated from the added phosphocreatine (PCr)/creatine (Cr) ratio:
where ΔG_ATP° is the standard ΔG_ATP (-7.592 kcal/mol), R is the gas constant (1.987 cal·K^-1·mol^-1), and T is temperature (310°K). Mitochondria oxidizing G+M at State 4 were given 2.5 mM PCr, 5 mM Cr, 5 mM ATP, and 75 U/ml CK, resulting in a J_o of ∼2/3 of State 3. Subsequent additions of PCr (to 3.75, 5, 7.5, and 10 mM) were made to slow J_o to ∼1/3 of State 3.
Additional experiments with pyruvate were conducted similar to those from Messer et al. (28 (link)). Briefly, mitochondria in the presence of saturating M were exposed to the CK clamp as described above with a PCr/Cr ratio of 0.25. Pyruvate was then titrated in stepwise at 10, 25, 50, 100, and 500 μM to increase respiration. The novel aspect of this experiment was that upon reaching a steady state with 500 μM pyruvate, stepwise additions of PCr were then made to slow J_o. Thus, in a single experiment, J_o was changed by both “Push” (pyruvate titration) and “Pull” (CK clamp) mechanisms.

Glancy B., Willis W.T., Chess D.J, & Balaban R.S. (2013). Effect of Calcium on the Oxidative Phosphorylation Cascade in Skeletal Muscle Mitochondria. Biochemistry, 52(16), 2793-2809.

Publication 2013

Anoxia austin Cell Respiration Creatine Creatine Kinase Glutamates Hydrolysis malate Mitochondria Oxygen Phosphates Phosphocreatine Pyruvate Sodium Titrimetry

Pancreas and Islet Tissue Characterization

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Publication 2016

Adult Anoxia Biological Assay Chromatin Immunoprecipitation Sequencing Diabetes Mellitus Donor, Organ Donors Ethics Committees, Research Homo sapiens Insulin Pancreas RNA-Seq secretion Tissues Wounds and Injuries

Anaerobic Dissimilatory Sulfur Metabolism

The materials and methods used in this work are described in detail in SI Appendix, Materials and Methods. The Bilophila and Desulfovibrio strains were grown in carbonate-buffered mineral salts medium reduced with Ti(III)-nitrilotriacetate. Cell-free extracts were prepared by French press disruption followed by centrifugation to remove unbroken cells; these extracts were used for proteomics analysis and measurement of Tpa, SarD, and IslAB activity. Sulfite was detected by a colorimetric (fuchsin) assay as well as by HPLC after derivatization, and acetaldehyde was detected by HPLC after derivatization. A hydrophilic interaction liquid chromatography column and HPLC-MS system was used to detect taurine, alanine, sulfoacetaldehyde, and isethionate. His-tagged Tpa and SarD were produced using E. coli Rosetta 2 DE3 and the His-tagged GREs, AdhE, and DctP using E. coli BL21. The His-tagged GRE activating enzymes were overexpressed in E. coli BL21(DE3) ΔiscR::kan. Before induction, these cultures were rendered anoxic by sparging with argon. Cell lysis and enzyme purification were also done under anoxic conditions. The recombinant GREs were rendered anoxic after purification and activated by incubation in the presence of the GRE-activating enzyme, SAM, and acriflavine as a photosensitizer in Hepes-bicine buffer under ambient light. Kinetics and substrate ranges of the GREs were measured spectrophotometrically using a coupled assay with alcohol dehydrogenase, reducing the acetaldehyde to ethanol concomitant with NADH formation.

Peck S.C., Denger K., Burrichter A., Irwin S.M., Balskus E.P, & Schleheck D. (2019). A glycyl radical enzyme enables hydrogen sulfide production by the human intestinal bacterium Bilophila wadsworthia. Proceedings of the National Academy of Sciences of the United States of America, 116(8), 3171-3176.

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Publication 2019

2'-deoxycytidine 5'-triphosphate Acetaldehyde Acriflavine Alanine Anoxia Argon Bilophila Biological Assay Buffers Carbonates Cell Extracts Cells Centrifugation Colorimetry Dehydrogenase, Alcohol Desulfovibrio Enzymes Escherichia coli Ethanol HEPES High-Performance Liquid Chromatographies Hydrophilic Interactions Kinetics Light Liquid Chromatography Minerals N,N-bis(2-hydroxyethyl)glycine NADH Photosensitizing Agents Rosaniline Dyes Salts Strains Sulfites sulfoacetaldehyde Taurine

Extreme Microbes from Anoxic Brine Lakes

Three extremophilic microbes, previously isolated from the deep-sea anoxic brine lakes, were selected as part of a genome-sequencing project due to their phylogenetic position, peculiar features and unique biotope. Analysis of their draft genomes provides us with a first glimpse on some of their unusual characteristics and the ways they cope with living in such a harsh environment [11 (link)-13 (link)].

Alam I., Antunes A., Kamau A.A., Ba alawi W., Kalkatawi M., Stingl U, & Bajic V.B. (2013). INDIGO – INtegrated Data Warehouse of MIcrobial GenOmes with Examples from the Red Sea Extremophiles. PLoS ONE, 8(12), e82210.

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Publication 2013

Anoxia brine Extremophiles Genome

Most recents protocols related to «Anoxia»

Anaerobic Cultivation of Gut Bacteria

Gordonibacter urolithinfaciens DSM
27213^T, Ellagibacter isourolithinifaciens DSM 104140^T obtained from the DSMZ culture collection,
and the isolated strain E. bolteae CEBAS
S4A9 were cultivated anaerobically in 5 mL WAM tubes. First, 2 mL
of a diluted aliquot of G. urolithinfaciens DSM 27213^T and E. bolteae CEBAS S4A9 strains was transferred to WAM (100 mL). Similarly, 2
mL of diluted aliquots of E. isourolithinifaciens DSM 104140^T and E. bolteae CEBAS S4A9 strains was transferred to WAM (100 mL). Finally, EA
dissolved in propylene glycol was added to the 100 mL cultures to
obtain a final concentration of 25 μM. During incubation in
an anoxic environment at 37 °C, aliquots (5 mL) were taken for
HPLC analyses as described below. Incubations were made in triplicate,
and the experiment was repeated twice.

Iglesias-Aguirre C.E., García-Villalba R., Beltrán D., Frutos-Lisón M.D., Espín J.C., Tomás-Barberán F.A, & Selma M.V. (2023). Gut Bacteria Involved in Ellagic Acid Metabolism To Yield Human Urolithin Metabotypes Revealed. Journal of Agricultural and Food Chemistry, 71(9), 4029-4035.

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Publication 2023

Anoxia Ellagibacter isourolithinifaciens Gordonibacter urolithinfaciens Propylene Glycol Strains

Urocanate Production by Enterocloster Strains

The
isolated strain E. bolteae CEBAS S4A9
and representative strains of the closest relatives (E. bolteae DSM 29485, DSM 15670^T, Enterocloster asparagiformis DSM 15981^T, Enterocloster citroniae DSM 19261^T, and Enterocloster clostridioformis DSM 933^T) obtained from the DSMZ culture collection were
used to investigate their capacity to produce final Uros in the presence
of EA and other Uro intermediaries. Briefly, isolated and DSMZ strains
were separately incubated on a WAM agar plate for 6 days. A single
colony was cultivated in a 5 mL WAM tube. Diluted inoculum (2 mL)
was transferred to WAM (20 mL), obtaining an initial load of 10⁴ CFU mL^–1. EA, Uro-M6, Uro-D, Uro-C, Uro-A,
IsoUro-A, and Uro-B were dissolved in propylene glycol and added to
the 20 mL cultures to obtain a final concentration of 15 μM
each. After incubation in an anoxic environment at 37 °C, aliquots
(5 mL) were taken periodically for high-performance liquid chromatography
(HPLC) analyses as described below.

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Publication 2023

Agar Anoxia Clostridium asparagiforme Clostridium citroniae Clostridium clostridioforme High-Performance Liquid Chromatographies Propylene Glycol Strains

Freshwater Sediment Microbial Profiling

Black freshwater sediment was retrieved, in June 2019 (TS1) and October 2020 (TS2), from a pond at Aarhus University campus (Vennelyst Park), Denmark (56.164672, 10.207908) at a water depth of 0.5–1 m. The sediment was stored with overlying water at 15°C for 2 weeks.
Before inoculation, sediment was homogenized, sieved (pore size: 0.5 mm) and autoclaved in 2 L bottles for 20 min as described in Thorup et al. (2021) (link) and Marzocchi et al. (2022) (link). Cooled down sediment (15°C) was distributed into 20 and 10 ethanol-cleaned Plexiglas core liners that were closed with a rubber stopper at the bottom. After 24 h settling time, the stoppers were pushed upwards to align the sediment surface with the core liner edge. The cores were inoculated by transferring a clump of sediment from a two-week-old, pre-grown single-strain enrichment culture of Ca. Electronema aureum GS (Thorup et al., 2021 (link)) and submerged in an aquarium with autoclaved tap water. The aquarium was covered with aluminum foil to prevent algae formation, equipped with aeration and a lid to prevent excessive evaporation, and kept at 15°C. Overlying water was replenished and refreshed several times during the incubation periods.
During the first time series (TS1), O₂, pH, and EP profiles were measured combined with 16S rRNA sequencing and microscopy observations over 80 days, due to technical issues, the videos could not be used for flocking observations. The sequencing and microscopy observations were repeated in the second time series (TS2) with O₂ and EP measurements over 81 days, and additionally the pH was measured on day 81. Following microsensor measurements (of both TS1 and TS2), 1–2 of the measured cores were sliced based on geochemical zone: the oxic layer (determined by the oxygen penetration depth) and the anoxic layer (below the oxygen penetration depth). Samples for light microscopy and 16S rRNA amplicon sequencing were taken from the anoxic layer of each core, of which the latter were frozen at −80°C until RNA extraction.

Lustermans J.J., Bjerg J.J., Burdorf L.D., Nielsen L.P., Schramm A, & Marshall I.P. (2023). Persistent flocks of diverse motile bacteria in long-term incubations of electron-conducting cable bacteria, Candidatus Electronema aureum. Frontiers in Microbiology, 14, 1008293.

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Publication 2023

Aluminum Anoxia Dental Cavity Liner Ethanol Freezing Light Microscopy Microscopy Oxygen Plexiglas RNA, Ribosomal, 16S Rubber Strains Vaccination

Microprofile Measurements of Cable Bacteria

At each time point, profiles were made of 1–3 randomly chosen core(s). Microprofiles were measured, with the aeration turned off, by moving in-house made O₂, pH and EP microsensors (Revsbech and Jørgensen, 1986 ; Revsbech, 1989 (link); Damgaard et al., 2014 (link)) stepwise (100–500 μm) downwards from ~3,000 μm above the sediment–water interface (SWI) to a depth of ~35,000 μm using a motorized micromanipulator (Unisense A/S, Denmark). At each step there was a 2 s waiting time before measuring a value for 2 s. All profiles were acquired using a four-channel multimeter with built-in A/D converter (Unisense). For the EP and pH sensors an in-house made millivoltmeter (resistance >10¹⁴ Ω,), and for the O₂ sensor a picoamperemeter (Unisense) that was digitized with a 16-bit A/D converter (ADC-216, Unisense), was used. The software SensorTrace PRO (Unisense) was used to control the micromanipulator and acquire measurements.
EP was used as a proxy for cable bacteria activity (Damgaard et al., 2014 (link)) and was measured downwards combined with an upwards profile. EP and pH profiles were measured against a general-purpose reference electrode (REF201 Radiometer Analytical, Denmark).
O₂ profiles were calibrated using Na-ascorbate in alkaline anoxic tap water and air-saturated (15°C) overlying water. The pH sensor was calibrated by a 3-point calibration (pH 4.0, 7.0, 10.0) with AVS TITRINORM buffers (VWR Chemicals, Denmark), and pH profiles were always measured in the dark. The reach of the anoxic zones was derived by determining the oxygen penetration depth from the O₂ measurements after the profiles were adjusted to the SWI. Two EP profiles (downwards and upwards) were made for each core, and were drift corrected, using the measurements from the overlying water and the time between the up and down profiles. Current densities were calculated per core and averaged per time point (1–3 profile pairs/cores), as showcased in Risgaard-Petersen et al. (2015) (link), using a sediment conductivity of 0.04 S m⁻¹ (water conductivity of 0.05 S m⁻¹ and sediment porosity of 0.87).
The cathodic oxygen consumption (COC) of cable bacteria, which is the contribution of the cable bacterial cathodic cells to the total oxygen consumption performed in the sediment, and the (electric) current density (mA m⁻²) in the sediment generated by the cable bacteria, used for this purpose were calculated as described in Risgaard-Petersen et al. (2015) (link) from the EP and O₂ microsensor measurements of the exact cores that were subsequently sampled for flocking observations.

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Publication 2023

Anoxia Bacteria Buffers Electric Conductivity Electricity Oxygen Consumption

Neurobehavioral Evaluation of Consciousness Disorders

As a part of routine clinical evaluation, patients were repeatedly evaluated with various neurobehavioural tests during their stay in the acute neurorehabilitation unit. In our analyses we used the total CRS-R score (0 = absence of any response, 23 = cognitively mediated behaviors) and the Disability rating scale (DRS) (Williams and Smith, 2017 (link)) scores (0 = no disability, 29 = extreme vegetative state) at discharge. The items in this scale correspond to the three original World Health Organization categories of impairment, disability, and handicap, and track a patient's functional and cognitive progress from coma to the community. In addition, the patients were also assessed with the Motor Behavior Tool – revised (MBT-r) (Jöhr et al., 2020 , Pincherle et al., 2019 (link)), a clinical evaluation tool for detecting subtle motor behavior that might reflect residual cognition in unresponsive patients. The patients with detected signs of motor behaviour are identified as patients with clinical cognitive motor dissociation. Experienced clinicians or neuropsychologists carried out the neurobehavioral evaluations. Patients’ demographic and clinical data are presented in Table 1.Table 1

Demographic and clinical data.

Subject	Sex	Age (years)	Interval Injury to MRI (days)	Interval MRI to discharge (days)	Etiology	CRS-R intitial	DRS at disch. (days)	CRS-R at disch. (days)
1	f	67	14	34	CVA	VS/UWS	5	23
2	m	24	8	45	CVA	VS/UWS	22	13
3	m	64	45	28	CVA	VS/UWS	22	4
4	f	57	9	11	CVA	MCS	17	15
5	f	72	101	28	CVA	MCS	25	5
6	m	73	16	41	CVA	VS/UWS	19	16
7	f	67	4	20	TBI	VS/UWS	9	23
8	m	37	1	42	ANOX	COMA	27	4
9	f	35	33	33	TBI	VS/UWS	21	7
10	m	60	21	10	TBI	VS/UWS	9	23
11	m	63	19	63	CVA	MCS	4	22
12	m	55	19	41	CVA	MCS	15	11
13	m	42	31	14	TBI	COMA	15	20
14	f	65	43	8	ANOX	COMA	7	23
15	m	27	287	16	TBI	VS/UWS	20	11
16	m	28	42	8	TBI	MCS	2	23
17	f	37	30	60	TBI	COMA	23	9
18	m	47	16	44	TBI	VS/UWS	7	22
19	f	66	30	28	TBI	COMA	11	23
20	f	39	34	20	CVA	COMA	11	21
21	f	52	23	27	CVA	MCS	15	21
22	m	61	35	27	CVA	COMA	14	18
23	m	61	34	34	CVA	COMA	11	23
24	m	78	50	41	ENC	COMA	15	13
25	m	44	28	26	TBI	COMA	11	21
26	f	60	26	49	CVA	COMA	22	11
27	f	69	41	59	ENC	MCS	18	11
28	f	54	30	25	TBI	VS/UWS	26	5
29	m	50	9	63	ANOX	MCS	8	22
30	f	84	26	14	TBI	COMA	21	13
31	m	16	20	12	TBI	MCS	6	23
32	m	35	19	25	LEUCO	VS/UWS	18	13
33	m	49	29	15	CVA	MCS-	6	21
34	m	72	22	13	CVA	MCS-	7	22
35	m	55	41	47	CVA	COMA	29	22
36	f	58	63	−5	CVA	VS/UWS	9	20
37	f	25	38	7	TBI	VS/UWS	11	11
38	m	73	20	37	TBI	VS/UWS	7	22
39	m	60	38	20	CVA	VS/UWS	11	22
40	m	59	43	3	ANOX	VS/UWS	23	8

CVA = cardiovascular accident, TBI = traumatic brain injury, ANOX = anoxia, ENC = encephalopathy, LEUCO = leucoencephalopathy, VS/UWS = vegetative state or unresponsive wakefulness syndrome, MCS = minimally conscious state, DRS = Disability Rating Scale, CRS-R = Coma Recovery Scale – Revised.

Pozeg P., Alemán-Goméz Y., Jöhr J., Muresanu D., Pincherle A., Ryvlin P., Hagmann P., Diserens K, & Dunet V. (2023). Structural connectivity in recovery after coma: Connectome atlas approach. NeuroImage : Clinical, 37, 103358.

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Publication 2023

Accidents Anoxia Cardiovascular System Cognition Comatose Disabled Persons Encephalopathies Injuries Leukoencephalopathy Minimally Conscious State Neurological Rehabilitation Patient Discharge Patients Syndrome Traumatic Brain Injury Vegetative State Wakefulness

Top products related to «Anoxia»

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Anaerobic chamber by Coy Laboratory Products

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The Anaerobic Chamber is a laboratory equipment designed to provide a controlled, oxygen-free environment for various applications that require an anaerobic atmosphere. It maintains a low-oxygen, high-nitrogen or carbon dioxide atmosphere to support the growth and handling of anaerobic organisms or to perform experiments and procedures that require an anaerobic environment.

Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific

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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

C57bl 6 mice by Charles River Laboratories

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The C57BL/6 mouse is a widely used inbred mouse strain. It is a common laboratory mouse model utilized for a variety of research applications.

Penicillin streptomycin by Thermo Fisher Scientific

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Hypoxia incubator chamber by STEMCELL

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The Hypoxia Incubator Chamber is a laboratory equipment designed to provide a controlled low-oxygen environment for cell culture and experimentation. It maintains a specified oxygen concentration within the chamber, allowing researchers to study cellular responses to hypoxic conditions.

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Ja 10 fixed angle rotor by Beckman Coulter

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H9c2 cell by American Type Culture Collection

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Phosphate buffered saline (pbs) by Thermo Fisher Scientific

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What is Anoxia and how can it impact research?

Anoxia is a condition characterized by a complete lack of oxygen supply to tissues, which can lead to severe tissue damage and organ failure. This lack of oxygen can have major implications for research, as it can compromise the accuracy and reproducibility of experiments involving Anoxia. Researchers need to be aware of the potential impact of Anoxia on their studies and take steps to mitigate these effects.

How can PubCompare.ai help with Anoxia research?

PubCompare.ai is an AI-powered platform that can significantly enhance the reproducibility and accuracy of Anoxia studies. The platform allows researchers to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. By helping researchers identify the most effective protocols related to Anoxia for their specific research goals, PubCompare.ai's AI-driven analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the best option for their work.

What are the different types or variations of Anoxia?

Anoxia can manifest in several different forms, including hypoxic anoxia (lack of oxygen in the air), ischemic anoxia (lack of blood flow), and anemic anoxia (lack of oxygen-carrying capacity in the blood). Each of these variations can have unique impacts on research and requires tailored approaches to mitigate the effects. Researchers should familiarize themselves with the specific type of Anoxia they are dealing with to ensure the most appropriate experimental design and protocols.

How can PubCompare.ai help optimize Anoxia research and drive scientific discovery?

By leveraging PubCompare.ai's advanced comparison capabilities, researchers can idenfify the best protocols and products from literature, pre-prints, and patents to optimize their Anoxia research. The platform's AI-powered analysis helps researchers pinpoint the most effective approaches, enhancing reproducibility and accuracy. This, in turn, can drive scientific discovery by enabling researchers to focus on the most promising avenues of inquiry and avoid the pitfalls associated with suboptimal experimental protocols.

What are some common challenges in using Anoxia in research, and how can PubCompare.ai help address them?

One of the key challenges in using Anoxia in research is ensuring consistent and reliable oxygen deprivation across experiments. Variations in oxygen levels, duration of exposure, and other factors can lead to inconsistent results. PubCompare.ai can help address this challeneg by providing researchers with access to the most rigorously tested and validated protocols for inducing Anoxia, allowing them to miminize variability and improve the quality of their findings. The platform's AI-powered analysis can also help identify the optimal experimental conditions and parameters for specific research objectives.

More about "Anoxia"

Anoxia, also known as hypoxia, is a condition characterized by a complete lack of oxygen supply to tissues, leading to severe tissue damage and organ failure.
Researchers can leverage PubCompare.ai, an AI-powered platform, to enhance the reproducibility and accuracy of their anoxia studies.
The platform helps researchers locate the best protocols and products from literature, pre-prints, and patents, enabling them to optimize their anoxia research and drive scientific discovery.
Advanced comparisons provided by PubCompare.ai can enhance the accuracy and reproducibility of anoxia studies.
When studying anoxia, researchers often employ various cell lines and animal models, such as H9c2 cells and C57BL/6 mice.
Maintaining optimal cell culture conditions, including the use of Fetal Bovine Serum (FBS), Dulbecco's Modified Eagle's Medium (DMEM), and antibiotics like Penicillin/Streptomycin, is crucial for maintaining cell viability and ensuring the reliability of experimental results.
Additionally, specialized equipment like anaerobic chambers and hypoxia incubator chambers can be utilized to simulate and study the effects of oxygen deprivation on cells and tissues.
Centrifugation techniques, such as the JA-10 Fixed-Angle Rotor, may also be employed to isolate and purify cellular components for further analysis.
By leveraging the insights and tools provided by PubCompare.ai, researchers can enhance the reproducibility and accuracy of their anoxia studies, ultimately leading to more robust scientific discoveries and advancing our understanding of this critical condition.