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Rana catesbeiana

Rana catesbeiana, also known as the American bullfrog, is a large frog species native to eastern North America.
It is a popular subject for biological research, with a wide range of published protocols and products available.
PubCompare.ai's AI-driven research tool makes it easy to discover, compare, and optimize protocols from literature, preprints, and patents, ensuring you can find the best approaches for your Rana catesbeiana studies.
Leveraging cutting-edge technology, PubCompare.ai helps streamline your workflow and maximize the efficiency of your research on this important amphibian species.

Most cited protocols related to «Rana catesbeiana»

1
Engineered Hybrid Ferritin and Encapsulin Proteins

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Publication 2015
Bos taurus Cloning Vectors Ferritin Genes Helicobacter pylori Hexosaminidase A Histidine Homo sapiens Hybrids Mammals Mutation Prolactin Protein Glycosylation Proteins Protein Subunits Rana catesbeiana Salts Strains TNFSF14 protein, human
2
Persistence of Environmental DNA
Two species were used for assessing the persistence of detectable amounts of DNA. Bullfrog tadpoles were studied to validate the possibility of integrating the approach of Ficetola et al. [5] in the species eradication strategy. Due to the risk of invasion and/or pathogen transmission to native populations (e.g. Batrachochytrium dendrobatidis[12] and Ranavirus [13] (link)) bullfrog tadpoles cannot be used outdoors, and the experiments were performed in aquariums (as in Ficetola et al. [5] ). The siberian sturgeon was used as DNA source species to test field conditions and placed in artificial ponds created about 20 years ago on the University campus, where this species has never been present.
For the bullfrog experiment, 3 different densities of tadpoles were used. One, 5 and 10 tadpoles were reared in 900 mL glass beakers for 5 days and each density was replicated 5 times. A 900 mL glass beaker without tadpoles was used as control. At the fifth day, the tadpoles were removed. At this time and every 24 h during 20 days, 15 mL of water were sampled from each glass beaker. Room temperature was maintained constant throughout the experimental period and the water temperature measured in the glass beakers was 17±1°C.
For the sturgeon experiment, three ponds of dimensions 12 m2 and 0.40 m deep were used. In each pond, a sturgeon (20 cm long) was housed for 10 days (from November the 04th to 13th 2009). On the tenth day, the sturgeons were removed and 15 mL of water were sampled from each pond. Water samples were collected every 24 h during 14 days. Water temperature fluctuated from 8 to 11°C during this period.
The duration of each experiment was determined after a preliminary test on the same condition without replication. In both experiments, water samples were added to a solution composed of 1.5 mL of sodium acetate 3 M, and 33 mL absolute ethanol immediately after collection, and then stored at −20°C until the DNA extraction.
Dejean T., Valentini A., Duparc A., Pellier-Cuit S., Pompanon F., Taberlet P, & Miaud C. (2011). Persistence of Environmental DNA in Freshwater Ecosystems. PLoS ONE, 6(8), e23398.
Publication 2011
Batrachochytrium dendrobatidis DNA Replication Ethanol Population Group Rana catesbeiana Ranavirus Sodium Acetate Tadpole Transmission, Communicable Disease
3
Immunolabeling of Bullfrog Saccular Hair Cells
Bullfrog sacculi were fixed in 3% paraformaldehyde in cold PBS for 25 min, washed in PBS, and permeabilized in 0.1% TX-100 in PBS for 1 h. Following a PBS wash, the tissue was blocked in 5% goat serum in PBS for 1 h and incubated overnight at 4°C in 4.5 μg/ml HCS-1 antibody in blocking solution. Bound antibodies were detected with Alexa-488 goat anti-mouse secondary antibody (Invitrogen), and filamentous actin was labeled with 33 nM Alexa-568 phalloidin (Invitrogen). Samples were mounted in Vectashield (Vector Laboratories, Burlingame, CA, USA) and viewed with a LSM 510 META confocal microscope (Zeiss). Isolated bullfrog saccular hair cells (Lumpkin and Hudspeth 1995 (link)) were allowed to settle onto coverslip dishes (Mattek, Ashland, MA, USA) coated with 1 mg/ml concanavalin A. The cells were fixed for 25 min in 3% paraformaldehyde in cold PBS, washed for 10 min in PBS, and permeabilized for 20 min in 0.1% saponin in PBS. Dishes were washed for 5 min in PBS and blocked for 1 h in 10 mg/ml bovine serum albumin (fraction V; EMD Biosciences, San Diego, CA, USA) and 5% goat serum in PBS. Primary antibody solutions of 4.5 μg/ml HCS-1 antibody with or without 2.5 μg/ml F2b anti-plasma membrane Ca2+ ATPase b isoform (anti-PMCAb) antibody (Dumont et al. 2001 (link); gift from P. Gillespie, Oregon Health & Science University) in blocking solution were incubated with the cells overnight at room temperature. Following two 10-min washes with PBS/0.1% Tween-20, dishes were incubated at room temperature for 2 h with blocking solution containing 33 nM Alexa-568 phalloidin and either 13 μg/ml Alexa 488 goat anti-mouse antibody alone to detect bound mAb HCS-1 or 13 μg/ml goat anti-mouse Alexa 488 and 13 μg/ml Alexa-568 goat anti-rabbit (Invitrogen) to visualize both otoferlin and PMCAb. Dishes were then washed 2 × 10 min in PBS/0.1% Tween-20 and 1 × 10 min in PBS and mounted in Vectashield. Images were obtained using an LSM 510 META confocal microscope with a 100×, 1.4 NA objective.
Goodyear R.J., Legan P.K., Christiansen J.R., Xia B., Korchagina J., Gale J.E., Warchol M.E., Corwin J.T, & Richardson G.P. (2010). Identification of the Hair Cell Soma-1 Antigen, HCS-1, as Otoferlin. JARO: Journal of the Association for Research in Otolaryngology, 11(4), 573-586.
Publication 2010
alexa 568 Antibodies Antibodies, Anti-Idiotypic Auditory Hair Cell Cells Cloning Vectors Cold Temperature Concanavalin A DFNB9 protein, human F-Actin Goat Hyperostosis, Diffuse Idiopathic Skeletal Immunoglobulins Microscopy, Confocal Mus paraform Phalloidine Plasma Membrane Calcium-Transporting ATPases polyethylene glycol monooctylphenyl ether Protein Isoforms Rabbits Rana catesbeiana Saccule Saponin Serum Serum Albumin, Bovine Tissues Tween 20
4
Bullfrog Papilla Dissection and Recordings
All procedures followed university-approved animal care protocols and guidelines. Adult bullfrogs (Rana catesbeiana) were sedated in an ice bath for about 20 min, then double-pithed and decapitated. Amphibian papillae were dissected in oxygenated artificial perilymph containing (in millimolar): 120 NaCl, 2 KCl, 2 CaCl2, 3 D-glucose, 1 creatine, and 5 HEPES at pH 7.30 (240 mOsmol·kg−1). Hair cells and their innervating afferent fibers were exposed by procedures described previously (Keen and Hudspeth, 2006 (link)). During recordings, the preparation was placed in HEPES-buffered perilymph or perfused continuously at 2–3 ml/min with bicarbonate-buffered perilymph continuously bubbled with 95% O2 and 5% CO2 and containing (in millimolar): 95 NaCl, 2 KCl, 2 CaCl2, 1 MgCl2, 3 D-glucose, 1 creatine, 1 Na-pyruvate, and 25 NaHCO3 at pH 7.30 (230 mOsmol·kg−1). Tetrodotoxin was added to the recording solution at a concentration of 1 µM to block voltage-gated Na+ channels.
Li G.L., Keen E., Andor-Ardó D., Hudspeth A.J, & von Gersdorff H. (2009). The Unitary Event Underlying Multiquantal EPSCs at a Hair Cell's Ribbon Synapse. The Journal of Neuroscience, 29(23), 7558-7568.
Publication 2009
Adult Amphibians Animals Auditory Hair Cell Bath Bicarbonate, Sodium Bicarbonates Cardiac Arrest CARE protocol Creatine Glucose HEPES Magnesium Chloride Nipples Perilymph Pyruvate Rana catesbeiana Sodium Chloride Tetrodotoxin
5
Deuterostome Phylogenomics: Orthologous Marker Selection
We built upon a previous phylogenomic dataset [39 (link)] to select a curated set of 258 orthologous markers for deuterostomes. Alignments were complemented with sequences from the National Center for Biotechnology Information (NCBI) databases using a multiple best reciprocal hit approach implemented in the newly designed Forty-Two software [40 ]. Because 454 DNA sequence reads are characterized by sequencing errors typically disrupting the reading frame when translated into amino acids, alignments were verified by eye using the program ED from the MUST package [41 (link)]. Ambiguously aligned regions were excluded for each individual protein using Gblocks with medium default parameters [42 (link)] with a few subsequent manual refinements using NET from the MUST package to relax the fact that this automated approach is sometimes too conservative. This manual refinement step restored only 418 amino acid sites (i.e. 0.6% of the total alignment length). Potential environmental contaminations and cross-contaminations between our samples were also dealt with at the alignment stage by performing Basic Local Alignment Search Tool (BLAST) searches of each sequence against a taxon-rich reference database maintained for each curated gene alignment and were further sought by a visual examination of each individual gene phylogeny.
The concatenation of the resulting 258 amino acid alignments was constructed with SCaFoS [43 (link)] by defining 63 deuterostomian operational taxonomic units (OTUs) representing all major lineages. The taxon sampling included 18 tunicates, 34 vertebrates, and one cephalochordate, with seven echinoderms, two hemichordates, and one xenoturbellid as more distant outgroups. When several sequences were available for a given OTU, the slowest evolving one was selected by SCaFoS, according to maximum likelihood distances computed by TREE-PUZZLE [44 (link)] under a WAG+F model. The percentage of missing data per taxon was reduced by creating some chimerical sequences from closely related species (i.e. Eptatretus burgeri/ Myxine glutinosa, Petromyzon marinus/Lethenteron japonicum, Callorhinchus milii/ C. callorynchus, Latimeria menadoensis/L. chalumnae, Rana chensinensis/ R. catesbeiana, Alligator sinensis/ A. mississippiensis, Chrysemys picta/ Emys orbicularis/ Trachemys scripta, Patiria miniata/ P. pectinifera/ Solaster stimpsonii, Apostichopus japonicus/ Parastichopus parvimensis, Ophionotus victoriae/ Amphiura filiformis) and by retaining only proteins with at most 15 missing OTUs. The tunicate Microcosmus squamiger was excluded at this stage due to a high percentage of missing data resulting from the low number of contigs obtained in the assembly. The final alignment comprised 258 proteins and 63 taxa for 66,593 unambiguously aligned amino acid sites with 20% missing amino acid data.
Delsuc F., Philippe H., Tsagkogeorga G., Simion P., Tilak M.K., Turon X., López-Legentil S., Piette J., Lemaire P, & Douzery E.J. (2018). A phylogenomic framework and timescale for comparative studies of tunicates. BMC Biology, 16, 39.
Publication 2018
Alligators Amino Acids Asterina pectinifera Echinodermata Environmental Pollution Genes Genetic Testing Petromyzon marinus Proteins Rana catesbeiana Reading Frames Stichopus japonicus Trees Urochordata Vertebrates

Most recents protocols related to «Rana catesbeiana»

1
Optimized ddRAD Sequencing for Amphibian Genomes
To determine appropriate restriction enzyme combinations and insert size for ddRAD protocols, 500 ng of DNA for two samples from each clade (R. chiricahuensis and E. anthonyi) was double digested with four enzyme pairs: SphI + EcoRI, EcoRI + MspI, SphI + MluCl, and SphI + MspI (New England BioLabs), cleaned using handmade Serapure beads (see Rohland & Reich, 2012 ), and sent to the University of Texas at Austin Genomics Sequencing and Analysis Facility (GSAF) for fragment visualization using an Agilent 2100 Bioanalyzer (Agilent) and standard 2100 Expert Software. We selected the SphI + MluCl enzyme combination for both Epipedobates and Rana because they sheared reasonable subsets of the genomes (~1%) at a size range amenable to Illumina sequencing technology (~300 nt). Based on our Bioanalyzer results, we aimed to recover 0.98% of the genome in Epipedobates (size selection window: 275–325 nt; x¯ = 291 nt) and 1.21% of the genome in Rana (size selection window: 300–350 nt; x¯ = 314 nt). We estimated the genome size of Epipedobates as 9GB, based on the upper limit for the dendrobatid Oophaga (Rogers et al., 2018 (link)), and 6GB for Rana catesbeiana following Hammond et al. (2017 ). To target a coverage depth of 20×, we requested 7.27 and 5.55 million paired‐end reads (2 × 150 paired‐end reads) per sample for Epipedobates and Rana, respectively (Table 1; see also Supporting Information). Preliminary data now suggest that Epipedobates genomes are closer to 6GB in size (R. D. Tarvin, unpublished data), which would imply that fewer reads could have been requested. Library preparation was performed following Peterson et al. (2012 (link)), using handmade Sera‐mag Speedbeads for all but the final bead clean‐up step (in which Dynabeads were used). DNA was quantified using PicoGreen dsDNA quantitation, DNA was standardized, and size selection was accomplished using a Pippin Prep machine (using a 2% cassette). Pooled libraries (total concentrations of 0.92 ng/μL for Epipedobates and 1.91 ng/μL for Rana) were then sequenced at the GSAF on an Illumina HiSeq 4000.
Chambers E.A., Tarvin R.D., Santos J.C., Ron S.R., Betancourth‐Cundar M., Hillis D.M., Matz M.V, & Cannatella D.C. (2023). 2b or not 2b? 2bRAD is an effective alternative to ddRAD for phylogenomics. Ecology and Evolution, 13(3), e9842.
Publication 2023
austin Deoxyribonuclease EcoRI DNA, Double-Stranded DNA Library DNA Restriction Enzymes Enzymes Genome Illumina Sequencing PicoGreen Rana Rana catesbeiana Serum
2
Engineered Protein Nanoparticle Vaccine

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Publication 2023
Amino Acids Cells Extinction, Psychological Ferritin Freezing Gel Chromatography Helicobacter pylori Mutation Nitrogen Polysaccharides Protein Glycosylation Proteins Rana catesbeiana
3
Bullfrog Neuromuscular Preparation
Spinal bullfrogs were prepared under anesthesia in accordance with USDA and PHS guidelines and regulations following approval from the Institutional Animal Care and Use Committee at Drexel University as described previously (Kim et al. 2019 (link)). Bull frogs were anesthetized with 5% tricaine (MS-222, Sigma), spinalized, and decerebrated. The frog was placed on a support and Myomatrix arrays were implanted into the semimembranosus (SM) hindlimb muscle using the “intramuscular” method described above. Epidermal electrical stimulation at the heel dorsum (500 msec train of 1 msec, 5V biphasic pulses delivered at 40 Hz) or foot pinch was used to evoke reflexive motor activity.
Chung B., Zia M., Thomas K.A., Michaels J.A., Jacob A., Pack A., Williams M.J., Nagapudi K., Teng L.H., Arrambide E., Ouellette L., Oey N., Gibbs R., Anschutz P., Lu J., Wu Y., Kashefi M., Oya T., Kersten R., Mosberger A.C., O’Connell S., Wang R., Marques H., Mendes A.R., Lenschow C., Kondakath G., Kim J.J., Olson W., Quinn K.N., Perkins P., Gatto G., Thanawalla A., Coltman S., Kim T., Smith T., Binder-Markey B., Zaback M., Thompson C.K., Giszter S., Person A., Goulding M., Azim E., Thakor N., O’Connor D., Trimmer B., Lima S.Q., Carey M.R., Pandarinath C., Costa R.M., Pruszynski J.A., Bakir M, & Sober S.J. (2023). Myomatrix arrays for high-definition muscle recording. bioRxiv.
Publication Preprint 2023
Anesthesia Cattle Epidermis Foot Heel Hindlimb Institutional Animal Care and Use Committees MS-222 Pulses Rana Rana catesbeiana Semimembranosus Stimulations, Electric tricaine
4
Spinal Bullfrog Muscle Electrophysiology
Spinal bullfrogs were prepared under anesthesia in accordance with USDA and PHS guidelines and regulations following approval from the Institutional Animal Care and Use Committee at Drexel University as described previously 56 (link). Bull frogs were anesthetized with 5% tricaine (MS-222, Sigma), spinalized, and decerebrated. The frog was placed on a support and Myomatrix arrays were implanted into the semimembranosus (SM) hindlimb muscle using the “intramuscular” method described above. Epidermal electrical stimulation at the heel dorsum (500 msec train of 1 msec, 5V biphasic pulses delivered at 40 Hz) or foot pinch was used to evoke reflexive motor activity.
Chung B., Zia M., Thomas K.A., Michaels J.A., Jacob A., Pack A., Williams M.J., Nagapudi K., Teng L.H., Arrambide E., Ouellette L., Oey N., Gibbs R., Anschutz P., Lu J., Wu Y., Kashefi M., Oya T., Kersten R., Mosberger A.C., O’Connell S., Wang R., Marques H., Mendes A.R., Lenschow C., Kondakath G., Kim J.J., Olson W., Quinn K.N., Perkins P., Gatto G., Thanawalla A., Coltman S., Kim T., Smith T., Binder-Markey B., Zaback M., Thompson C.K., Giszter S., Person A., Goulding M., Azim E., Thakor N., O’Connor D., Trimmer B., Lima S.Q., Carey M.R., Pandarinath C., Costa R.M., Pruszynski J.A., Bakir M, & Sober S.J. (2023). Myomatrix arrays for high-definition muscle recording. bioRxiv.
Publication Preprint 2023
Anesthesia Cattle Epidermis Foot Heel Hindlimb Institutional Animal Care and Use Committees MS-222 Pulses Rana Rana catesbeiana Semimembranosus Stimulations, Electric tricaine
5
Aquaculture Mapping in Changxing County
Changxing County is part of Huzhou City, Zhejiang Province, China, between 30°43′–31°11′ N latitude and 119°33′–120°06′ E longitude, located in the Hangjiahu Plain of the Yangtze River Delta, southwest of Taihu Lake, the third largest freshwater lake in China. The rich water resources have created a good environment for local farmers to develop aquaculture, and the main elements of aquaculture are river crabs, crayfish, bullfrogs, etc. The study area (as shown in Figure 1) contains more river crab farming areas (pond), and it is important to obtain the crab pond area accurately and effectively in order to carry out the development of local aquaculture and yield estimation.
Nanxun District is located in the eastern part of Huzhou City, Zhejiang Province, China, in the southern part of Taihu Lake, with similar geotypes to Changxing County, and has abundant aquatic and agricultural crops.
Yan C., Fan X., Fan J., Yu L., Wang N., Chen L, & Li X. (2023). HyFormer: Hybrid Transformer and CNN for Pixel-Level Multispectral Image Land Cover Classification. International Journal of Environmental Research and Public Health, 20(4), 3059.
Publication 2023
Agricultural Crops Astacoidea Brachyura Farmers Louses, Crab Rana catesbeiana Rivers Water Resources

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More about "Rana catesbeiana"

The American bullfrog, scientifically known as Rana catesbeiana, is a large frog species native to the eastern regions of North America.
As a popular subject for biological research, the American bullfrog has a wide range of published protocols and products available to researchers.
PubCompare.ai's cutting-edge AI-driven research tool makes it easy to discover, compare, and optimize protocols from literature, preprints, and patents, ensuring researchers can find the best approaches for their Rana catesbeiana studies.
This powerful platform leverages the latest technology to streamline workflows and maximize the efficiency of research on this important amphibian species.
Researchers working with the American bullfrog may utilize various chemicals and reagents, such as MS-222 (also known as tricaine methanesulfonate) for anesthesia, Collagenase IV for tissue dissociation, Diethyl ether for euthanasia, Sodium thiosulfate and Sodium carbonate as buffers, and antioxidants like Butylhydroxytoluene (BHT) and Butylhydroxyanisole (BHA).
Additionally, Potassium biphthalate and Potassium dichromate may be employed in certain experimental protocols, while DMSO (Dimethyl sulfoxide) can be used as a solvent or cryoprotectant.
By harnessing the insights and capabilities of PubCompare.ai, researchers can optimize their workflows and enhance the efficiency of their Rana catesbeiana studies, leading to more impactful and meaningful scientific discoveries.