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Escherichia coli O157

Escherichia coli O157 is a pathogenic strain of the bacterium Escherichia coli, known for its association with severe foodborne illnesses.
This strain can produce Shiga toxin, leading to potentially life-threatening conditions such as hemorrhagic colitis and hemolytic uremic syndrome.
Researchers studying E. coli O157 can leverage the PubCompare.ai platform to streamline their workflow, identifying optimal protocols and products from the latest literature, preprints, and patents.
The AI-driven comparisons offered by PubCompare.ai can help researchers enhance their E. coli O157 studies and get more from their research effrots.

Most cited protocols related to «Escherichia coli O157»

1
Expanded DDH Benchmark Dataset for Genome Analysis
The DDH benchmark data set was extended compared to previous studies aiming at an increased precision and significance of the ranking of the genome-to-genome distance methods and the models for the conversion to DDH values. In detail, the here used data set (henceforth called “DS1”) comprised 156 unique genome pairs along with their respective DDH values: 62 from Goris et al. [6 (link)], 31 from the GOLD database [25 (link)], and 63 from Richter et al. [7 (link)]. Only the first two sources had been considered in a previous publication on GBDP as DDH replacement [8 (link)].
If several DDH/ANIb/ANIm/Tetra values were present for a single genome pair, they were averaged. A single genome pair showed a DDH value above 100% similarity (i.e., 100.9% between Escherichia coli O157:H7 EDL933 and Escherichia coli O157:H7 Sakai). As it biologically made not much sense this value was set to 100% to maintain proper input data for some of the statistical models (see below). Another genome pair (Thermotoga maritima MSB8 and Thermotoga petrophila RKU-1) had a contradicting relation between its DDH value (16.9%) and the genome based distance/similarity measures (GBDP, ANI, ANIb, ANIm and Tetra) on the other hand [7 (link)]. Following [7 (link)], this questionable data point was excluded from the correlation analyses. The full list of genome pairs used in this study is found in the Additional file 1.
To detect significant deviations, if any, between the new and the previous GBDP implementation, the data subset “DS2” was created, containing only the previously available data points [8 (link)]. For comparing GBDP with the first ANI implementation, data subset “DS3” comprised the 62 data points in common between [6 (link),8 (link)]; for comparison with the JSpecies study, subset “DS4” contained only the 98 DDH values in common between [7 (link),8 (link)].
Meier-Kolthoff J.P., Auch A.F., Klenk H.P, & Göker M. (2013). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics, 14, 60.
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Publication 2013
Escherichia coli O157 Genome Gold Tetragonopterus Thermotoga maritima Thermotoga petrophila
2
Benchmarking Bacterial Genome Assemblies
In total six bacterial datasets were used for testing the performance of the software. These comprise Illumina MiSeq, Roche-454 and PacBio RS reads from Escherichia coli (K12 MG1655), Escherichia coli (O157:H7 F8092B), Bibersteina trehalosi (USDA-ARS-USMARC-192), Mannheimia haemolytica (USDA-ARS-USMARC-2286), Francisella tularensis (99A-2628) and Salmonella enterica (Newport SN31241). Datasets are downloaded from http://www.cbcb.umd.edu/software/PBcR/closure/index.html and further described in Koren et al. (2013). Dataset statistics are displayed in Table 1. To assess the assembly correctness we used close reference genomes deposited in the NCBI database (E. coli K12 MG1655 = NC_000913, E. coli O157:H7 = NC_002127, NC_002128, NC_002695, F. tularensis = NC_008369, S. enterica = NC_011079, NC_011080, NC_009140). For B. trehalosi and M. haemolytica no reference genome is currently available.
Boetzer M, & Pirovano W. (2014). SSPACE-LongRead: scaffolding bacterial draft genomes using long read sequence information. BMC Bioinformatics, 15, 211.
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Publication 2014
Bacteria Escherichia coli Escherichia coli K12 Escherichia coli O157 Francisella tularensis Genome Mannheimia haemolytica Salmonella enterica
3
Evaluating GI Prediction Algorithms
To evaluate the ability of different software to predict well-defined GIs obtained by other groups using independent methods, a literature dataset was created by reviewing articles describing GIs in some well characterized organisms. The literature dataset from Langille et al. (Langille et al., 2008 (link)) was used and extended to include six genomes: Escherichia coli O157: H7 str. Sakai (NC_002695.1), Escherichia coli CFT073 (NC_004431.1), Salmonella enterica subsp. enterica serovar Typhi str. CT18 (NC_003198.1), Streptococcus pyogenes str. MGAS315 (NC_004070.1), Vibrio parahaemolyticus RIMD 2210633 (NC_004603.1) and Staphylococcus aureus str. MW2 (NC_003923.1). Two genomes from the literature dataset of Langille et al. were discarded due to changes in accession version number (NC_002655.2: Escherichia coli O157: H7 EDL933, NC_003197.1: Salmonella typhimurium LT2) that could have impacted the accuracy of GI coordinates. Overall, the literature dataset comprises 80 GIs ranging in size from 3 to 133 kb, encompassing over 3 Mbp in total (Supplementary Table S3).
Both the C-dataset and the L-dataset are available in tabular format as Supplementary table in this contribution. Tabular as well as fasta formats are also available on IslandViewer 4 website (http://www.pathogenomics.sfu.ca/islandviewer/download/).
Bertelli C, & Brinkman F.S. (2018). Improved genomic island predictions with IslandPath-DIMOB. Bioinformatics, 34(13), 2161-2167.
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Publication 2018
Escherichia coli Escherichia coli O157 Genome Salmonella typhi Salmonella typhimurium LT2 Staphylococcus aureus Streptococcus pyogenes Vibrio parahaemolyticus
4
Comparative Genomics: Curated Genome Sequence Database
The following genome sequence files were curated from the Genome Bioinformatics Group of University of California, Santa Cruz [25 ]: Human, March 2006 (hg18); Chimpanzee, March 2006 (panTro2); Rhesus, January 2006 (rheMac2); Rat, November 2004 (rn4); Mouse, February 2006 (mm8); Cat, March 2006 (felCat3); Dog, May 2005 (canFam2); Horse, January 2007 (equCab1); Cow, March 2005 (bosTau2); Opossum, January 2006 (monDom4); Chicken, May 2006 (galGal3); Xenopus tropicalis, August 2005 (xenTro2); Zebrafish, March 2006 (danRer4); Tetraodon, February 2004 (tetNig1); Fugu, October 2004 (fr2); Stickleback, February 2006 (gasAcu1); Medaka, April 2006 (oryLat1); D. melanogaster, April 2006 (dm3); D. simulans, April 2005 (droSim1); D. sechellia, October 2005 (droSec1); D. yakuba, November 2005 (droYak2); D. erecta, August 2005 (droEre1); D. ananassae, August 2005 (droAna2); D. pseudoobscura, November 2005 (dp3); D. persimilis, October 2005 (droPer1); D. virilis, August 2005 (droVir2); D. mojavensis, August 2005 (droMoj2); D. grimshawi, August 2005 (droGri1); C. elegans, January 2007 (ce4); C. brenneri, January 2007 (caePb1); C. briggsae, January 2007 (cb3); C. remanei, March 2006 (caeRem2); and P. pacificus, February 2007 (priPac1); The genome sequence files for the Elephant, June 2005; Hedgehog, June 2006 and Armadillo, June 2005 were downloaded from the Broad Institute [26 ].
The following bacteria genome sequence files were curated from the BacMap database of University of Alberta [27 ]: Staphylococcus aureus COL; Staphylococcus aureus MRSA252; Staphylococcus aureus MSSA476, Staphylococcus aureus Mu50; Staphylococcus aureus MW2; Staphylococcus aureus N315; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus RF122; Staphylococcus aureus subsp. aureus USA300; Staphylococcus epidermidis ATCC 12228; Staphylococcus epidermidis RP62; Staphylococcus haemolyticus JCSC1435; Escherichia coli 536; Escherichia coli APEC O1; Escherichia coli CFT073; Escherichia coli O157:H7 EDL933; Escherichia coli K12 MG1655; Escherichia coli W3110; Escherichia coli O157:H7 Sakai; Klebsiella pneumoniae MGH 78578; Salmonella enterica Choleraesuis SC-B67; Salmonella enterica Paratypi A ATCC 9150; Salmonella typhimurium LT2; Salmonella enterica CT18; Salmonella enterica Ty2; Shigella boydii Sb227; Shigella dysenteriae Sd197; Shigella flexneri 2a 2457T; and Shigella flexneri 301. The genome sequence files for Staphylococcus aureus subsp. aureus JH1, Staphylococcus aureus subsp. aureus JH9, Staphylococcus aureus Mu3, and Staphylococcus aureus subsp. aureus str. Newman were curated from the European Bioinformatics Institute of the European Molecular Biology Laboratory [28 ]. The genome sequence file for Escherichia coli UT189 was taken from Enteropathogen Resource Integration Center [29 ], and genome sequence data for Salmonella bongori was downloaded from the Sanger Institute Sequencing Centre [30 (link)].
The mosquito genome sequence files for Aedes aegypti, Anopheles gambiae and Culex pipiens were curated from the VectorBase database [31].
Yavatkar A.S., Lin Y., Ross J., Fann Y., Brody T, & Odenwald W.F. (2008). Rapid detection and curation of conserved DNA via enhanced-BLAT and EvoPrinterHD analysis. BMC Genomics, 9, 106.
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Publication 2008
Aedes Anopheles gambiae Armadillos Caenorhabditis elegans Chickens Culex Culicidae Didelphidae Drosophila melanogaster Drosophila simulans Elephants Equus caballus Erinaceidae Escherichia coli Escherichia coli K12 Escherichia coli O157 Europeans Genome Genome, Bacterial Homo sapiens Klebsiella pneumoniae Macaca mulatta Mice, House Oryziinae Pan troglodytes Salmonella bongori Salmonella enterica Salmonella typhimurium LT2 Shigella boydii Shigella dysenteriae Shigella flexneri Staphylococcus aureus Staphylococcus aureus subsp. aureus Staphylococcus epidermidis Staphylococcus haemolyticus Sticklebacks Takifugu Xenopus Zebrafish
5
Robust Bacterial Genome Reference Database
Central to our approach is a robust database against which to map the query sequencing reads. For the purposes of this demonstration, we gathered a database of 170 complete bacterial chromosomes obtained from 131 distinct strains (610 Mbp) (see the Supplemental Material for accession numbers for the genomes included in this reference database). The database was intended to aid in the identification of eight bacterial agents of bioterrorism identified by the CDC: Bacillus anthracis, Burkholderia mallei, Burkholderia pseudomallei, Brucella sp., Clostridium botulinum, Escherichia coli O157:H7, Francisella tularensis, and Yersinia pestis.
In order to differentiate closely related strains and species (often nonpathogenic) from target strains of interest, we wanted to include in our reference database genomes from any closely related strains/species. Therefore, closely related species/strains were identified by phylogenetic analysis of the 16S ribosomal RNA genes. 16S sequences for all eight pathogens of interest were obtained from GenBank and used to query the nr database utilizing BLASTN (Altschul et al. 1997 (link)) using default parameters (Word Size: 28, Expect Value: 10, Match/Mismatch Scores: 1, −2, Gap Costs: Linear). We identified 3206 sequences corresponding to 1050 named species or subspecies with multiple sequences represented within a number of these taxonomic groups using a partial or full match with BLASTN. We then estimated phylogenetic relationships amongst these sequences and our target species. From this phylogeny, we selected 131 completed genome sequences, 332 fully sequenced plasmids, and 207 whole-genome shotgun sequencing projects to serve as our reference database (see the Supplemental Material for details). Although this study uses the entire genome database, any subset of these sequence types could be used for reference material. The genetic distances for Figure 1 were calculated by performing an all-against-all BLAST as implemented previously (Agren et al. 2012 (link)). Strains of the same species that were >98% similar using this metric were considered “closely related” strains for Figure 1.
Francis O.E., Bendall M., Manimaran S., Hong C., Clement N.L., Castro-Nallar E., Snell Q., Schaalje G.B., Clement M.J., Crandall K.A, & Johnson W.E. (2013). Pathoscope: Species identification and strain attribution with unassembled sequencing data. Genome Research, 23(10), 1721-1729.
Publication 2013
Bacillus anthracis Bacteria Biological Warfare Agents Brucella Burkholderia mallei Burkholderia pseudomallei Chromosomes, Bacterial Clostridium botulinum Escherichia coli O157 Francisella tularensis Genome Pathogenicity Plasmids Reproduction Ribosomal RNA Genes Strains Yersinia pestis

Most recents protocols related to «Escherichia coli O157»

1
Evaluating EHEC O157:H7 Adherence to HEp-2 Cells
Not available on PMC !

Example 5

In order to compare levels of adherence to HEp-2 epithelial cells in culture, we used an established model for evaluating adherence of EHEC O157:H7 (27). HEp-2, human laryngeal carcinoma epithelial cells, were a kind gift from Dr. Carlton Gyles (Department of Pathobiology, University of Guelph). Briefly, HEp-2 cells grown in EMEM supplemented with 10% (v/v) FBS were plated onto 24-well tissue culture plates at 2×105 cells ml−1 and incubated for 24 h in the presence of 5% CO2. The cells were then maintained during the assay in serum and antibiotic-free EMEM. Before inoculation with bacteria, 10% (v/v) of L. acidophilus CFSM selected fractions were added in triplicate to treatment group wells. Wells containing the negative control groups were inoculated with 105 E. coli O157:H7 strain VS94 with or without supplementation with 100 μM propanolol (Sigma-Aldrich Canada Ltd.). Following inoculation of 105 EHEC O157 into treatment and control group wells, the plates were incubated for 3 h at 37° C. in the presence of 5% CO2. The cell monolayers were then washed three times with PBS to remove non-adhering bacteria and fresh medium was added. Cells were incubated for another 3 h and then washed six times with PBS. Washed cells were lysed with 0.1% Triton X-100. Released bacteria present in the suspension were collected and appropriate dilutions were plated on LB agar. To evaluate if the percentage of adherence in the treatment groups was significantly different from that of the control group, where the recovered counts from the control group (2.2×107 CFU ml−1) were considered to be 100%, the percentage of adherence in the negative control and treatment groups were calculated using the following equation.

% ⁢ ⁢ of ⁢ ⁢ Recovery ⁢ = Group _ 2.2 × 10 7 ⁢ ⁢ CFU ⁢ ⁢ ml - 1 ⁢ × 100 _

US11857581B2. Antiviral methods and compositions comprising probiotic bacterial molecules (2024-01-02). MicroSintesis Inc. [CA]. Inventors: Mansel Griffiths [CA].
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Patent 2024
Agar Antibiotics Bacteria Bacterial Vaccines Biological Assay Carcinoma Cell Adhesion Cell Culture Techniques Cells Enterohemorrhagic Escherichia coli Epithelial Cells Escherichia coli O157 Homo sapiens Lactobacillus acidophilus Larynx Propranolol Serum Strains Technique, Dilution Tissues Triton X-100 Vaccination
2
Antimicrobial Assay Protocols for Diverse Microbes
Microorganisms used in this study included L. enzymogenes ATCC 29487, methicillin-sensitive S. aureus (MSSA) (ATCC 25923), methicillin-resistance S. aureus (MRSA) (ATCC 33591) and E. coli O157:H7. Culture media used included brain heart infusion (BHI) agar and broth obtained from Sigma- Aldrich (India) and nutrient agar (NA) obtained from Scharlau (European Union). Sterile syringe filters (0.22 μm) were purchased from Millipore (Amman, Jordan). Antibiotics (cefixime, gentamicin and levofloxacin) were kindly donated by JOSWE® Medical and Dar Al Dawa® pharmaceutical company, Amman-Jordan.
L. enzymogenes bacterial pellets were rehydrated aseptically using 5 mL of 10% strength tryptone soy broth (TSB), incubated at 28°C and shaken at 200 rpm for 3 days in a shaker incubator (MS, Taiwan) [44 (link)]. Then, L. enzymogenes culture (200 μL) was seeded over 10% strength of tryptone soy agar (TSA) plate and incubated at 28°C for 3 days. L. enzymogenes pure broth culture (PBC) was prepared by transferring a full loop from the stock plate to a 50 mL sterile falcon containing 10% TSB, followed by incubation at 28°C for 3 days. E. coli O157:H7 was cultured in nutrient broth (NB) (Biolab); MSSA and MRSA in brain heart infusion (BHI) broth (Biolab).
Suaifan G.A., Abdel Rahman D.M., Abu-Odeh A.M., Abu Jbara F., Shehadeh M.B, & Darwish R.M. (2023). Antibiotic—Lysobacter enzymogenes proteases combination as a novel virulence attenuating therapy. PLOS ONE, 18(3), e0282705.
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Publication 2023
A-Loop Agar Antibiotics, Antitubercular Bacteria Brain Cefixime Culture Media Escherichia coli O157 Gentamicin Heart Levofloxacin Methicillin Methicillin-Resistant Staphylococcus aureus Methicillin Resistance Nutrients Pellets, Drug Pharmaceutical Preparations Sterility, Reproductive Syringes
3
Microplate Assay for Assessing Antimicrobial Efficacy
A microplate growth inhibition assay [47 (link)] was applied to measure the growth inhibitory effect of L. enzymogenes CFS, antibiotics (cefixime, levofloxacin and gentamicin) and their combination against MSSA, MRSA and E. coli O157:H7. This assay allows the observation of discernible inhibition during growth using turbidity parameter which is measured through the detection of light scatter in absorbance at 600 nm using a microplate reader.
L. enzymogenes CFS growth inhibitory activity evaluation was performed by adding 10 μL aliquots of 106 CFU/mL bacterial suspension (MSSA, MRSA and E. coli O157:H7) to sterile microtiter plate wells containing 10 μL L. enzymogenes CFS (proteolytic activity 0.145 unit/mL) and 180 μL broth.
For the evaluation of antibiotics’ antibacterial activity, the MIC, 0.5 MIC, 0.25 MIC and 0.125 MIC concentrations for each antibiotic (cefixime, levofloxacin and gentamicin) were prepared. The test was carried out by placing 180 μL BHI for S. aureus and NB for E. coli O157:H7 and 10 μL of the prepared antibiotic dilution in each well, then 10 μL aliquot of the pathogen (106 CFU/ml) was added.
For the combination study, 10 μL of the bacterial suspension (106 CFU/ml) was added to wells containing 170 μL broth, 10 μL of L. enzymogenes CFS and 10 μL of each prepared antibiotic concentration (MIC, 0.5 MIC, 0.25 MIC and 0.125 MIC).
As a full growth control run, a 10 μL aliquot of the pathogenic cell suspension was inoculated at 106 CFU/mL into 190 μL of the corresponding sterile broth. Also, a test blank was run with each experiment where the 10 μL of the pathogen was replaced by the proper sterile broth.
In the above-mentioned experiments, the microtiter plate was incubated at 37°C for 24 h and the optical density (OD) was measured at 600 nm using a microplate reader (Epoch-Biotec, California, USA). Results were calculated as the average mean of three readings.
In this study, antibacterial activity was expressed as percentage inhibition of bacterial growth following 24 h incubation at 37 °C and calculated using the following equation:
%GrowthInhibition=100-ODT-ODTBODFG-ODFB×100%
Where:
Suaifan G.A., Abdel Rahman D.M., Abu-Odeh A.M., Abu Jbara F., Shehadeh M.B, & Darwish R.M. (2023). Antibiotic—Lysobacter enzymogenes proteases combination as a novel virulence attenuating therapy. PLOS ONE, 18(3), e0282705.
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Publication 2023
Anti-Bacterial Agents Antibiotics Antibiotics, Antitubercular Bacteria Biological Assay Cefixime Cells EPOCH protocol Escherichia coli O157 Gentamicin Levofloxacin Light Methicillin-Resistant Staphylococcus aureus Pathogenicity Proteolysis Psychological Inhibition Staphylococcus aureus Sterility, Reproductive Technique, Dilution Vision
4
Antibiotic Susceptibility of Pathogenic Bacteria
The susceptibility of MSSA, MRSA and E. coli O157:H7 to the three antibiotics; cefixime, levofloxacin and gentamicin was performed using National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution method [46 ]. Minimum inhibitory concentration (MIC) was determined using 96 flat-bottom microtiter plates (TPP, Switzerland). Each test well was filled with 90 μL BHI for S. aureus and NB for E. coli O157:H7. An aliquot (100 μL) of the antibiotic stock solution was added to the test well and mixed. A series of twelve 2-fold serial dilutions of the antibiotics were examined. The concentration ranges used to determine MICs were: cefixime 256–0.125 μg/mL, levofloxacin 64–0.031μg/mL, gentamicin 128–0.062 μg/mL against S. aureus and cefixime 32–0.0156 μg/mL, levofloxacin 32–0.0156 μg/mL, gentamicin 64–0.031 μg/mL against E. coli O157:H7. All dilutions of the tested antibiotics were inoculated with 10 μL of 106 CFU/mL of the specified bacterial strain and then, incubated at 37 °C for 24 h. Positive control (broth and bacterial suspension) and negative control (broth only) wells were included in every experiment to prove adequate microbial growth and media sterility during the incubation period.
In the test wells, microbial growth was assessed visually from culture turbidity and compared to the negative and positive controls. MICs were determined as the lowest concentration of the antibiotic that inhibits the growth of the microorganism. The test was carried out in triplicate (in the same 96-well plate) and repeated twice for each bacterium.
Suaifan G.A., Abdel Rahman D.M., Abu-Odeh A.M., Abu Jbara F., Shehadeh M.B, & Darwish R.M. (2023). Antibiotic—Lysobacter enzymogenes proteases combination as a novel virulence attenuating therapy. PLOS ONE, 18(3), e0282705.
Full text: Click here
Publication 2023
Antibiotics Antibiotics, Antitubercular Bacteria Cardiac Arrest Cefixime Clinical Laboratory Techniques Escherichia coli O157 Gentamicin Levofloxacin Methicillin-Resistant Staphylococcus aureus Minimum Inhibitory Concentration Staphylococcus aureus Sterility, Reproductive Strains Susceptibility, Disease Technique, Dilution
5
Antimicrobial Activity of L. enzymogenes CFS
Growth inhibitory activity of L. enzymogenes CFS against MSSA, MRSA and E. coli O157:H7 PBCs (108 CFU/mL) grown on agar plates was investigated by spreading two different volumes (100 μL and 200 μL) of L. enzymogenes CFS (prepared from a 14-day PBC) over the plates (Fig 2).
Suaifan G.A., Abdel Rahman D.M., Abu-Odeh A.M., Abu Jbara F., Shehadeh M.B, & Darwish R.M. (2023). Antibiotic—Lysobacter enzymogenes proteases combination as a novel virulence attenuating therapy. PLOS ONE, 18(3), e0282705.
Full text: Click here
Publication 2023
Agar Escherichia coli O157 Methicillin-Resistant Staphylococcus aureus Psychological Inhibition

Top products related to «Escherichia coli O157»

1
E coli o157 h7 by American Type Culture Collection
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E. coli O157:H7 is a strain of the Escherichia coli bacterium. It is a Gram-negative, rod-shaped bacterium commonly found in the lower intestine of warm-blooded organisms, including humans.
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Staphylococcus aureus by American Type Culture Collection
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Staphylococcus aureus is a bacterial strain available in the American Type Culture Collection (ATCC) product portfolio. It is a Gram-positive, spherical-shaped bacterium commonly found in the human nasal passages and on the skin. This strain is widely used in research and laboratory settings for various applications.
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Pseudomonas aeruginosa by American Type Culture Collection
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Pseudomonas aeruginosa is a bacterial strain available from the American Type Culture Collection (ATCC). It is a Gram-negative, aerobic bacterium commonly found in soil and water environments. This strain can be used for various research and testing purposes.
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Enterococcus faecalis is a Gram-positive, facultatively anaerobic bacterium. It is commonly found in the human gastrointestinal tract and is known for its ability to survive in diverse environments.
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Tryptic soy broth (tsb) by BD
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Tryptic soy broth is a general-purpose microbiological culture medium used for the cultivation and growth of a wide range of bacteria. It provides the necessary nutrients, including peptones, glucose, and salts, to support the growth of a variety of bacterial species.
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Sorbitol macconkey agar by Thermo Fisher Scientific
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Sorbitol MacConkey agar is a selective and differential culture medium used for the isolation and identification of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli (STEC) from clinical and food samples. It contains sorbitol, which is fermented by most E. coli strains, except for E. coli O157:H7, which is unable to ferment sorbitol. This allows for the differentiation of E. coli O157:H7 from other E. coli strains.
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Escherichia coli o157 h7 by American Type Culture Collection
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Escherichia coli O157:H7 is a strain of the bacterium Escherichia coli. It is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms, including humans. This strain is characterized by the presence of specific antigens on its cell surface.
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E coli o157 latex test kit by Thermo Fisher Scientific
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The E. coli O157 latex test kit is a diagnostic tool used for the rapid detection of the E. coli O157 strain in food and environmental samples. The kit utilizes latex agglutination technology to provide a qualitative result for the presence or absence of the target pathogen.
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More about "Escherichia coli O157"

Escherichia coli (E. coli) O157 is a pathogenic strain of the common bacteria Escherichia coli, known for its association with severe foodborne illnesses.
This strain can produce Shiga toxin, leading to potentially life-threatening conditions such as hemorrhagic colitis and hemolytic uremic syndrome.
E. coli O157:H7 is a closely related variant that is also a major concern for public health.
Researchers studying E. coli O157 can leverage the PubCompare.ai platform to streamline their workflow, identifying optimal protocols and products from the latest literature, preprints, and patents.
The AI-driven comparisons offered by PubCompare.ai can help researchers enhance their E. coli O157 studies and get more from their research effrots.
Other bacteria like Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis are also important pathogens that can cause serious infections.
Tryptic soy broth and Sorbitol MacConkey agar are commonly used growth media for culturing and identifying E. coli O157 and related strains.
To support their research, scientists can utilize various tools and techniques, such as the E. coli O157 latex test kit, which provides a rapid and accurate way to detect the presence of this strain.
Plate count agar is another useful medium for enumerating and isolating E. coli and other bacteria.
By leveraging the latest technologies and resources, researchers can streamline their E. coli O157 studies, enhance their workflow, and gain valuable insights to better understand and address this important public health concern.