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Fusobacterium nucleatum

Fusobacterium nucleatum is an anaerobic, gram-negative bacterium commonly found in the human oral cavity.
It is associated with various oral and systemic diseases, including periodontal disease, colorectal cancer, and adverse pregnancy outcomes.
This versatile pathogen utilizes a range of virulence factors to adhere to host cells, evade immune responses, and promote inflammation.
Exploring the power of PubCompare.ai's AI-powered platform can enhance research reproducibility and accuracy in the study of F. nucleatum, helping users locate the best protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the most reliable and effective approaches.
Experience seamless research with PubCompare.ai's intuitive interface and cutting-edg technology.

Most cited protocols related to «Fusobacterium nucleatum»

1
Quantitative PCR Detection of Fusobacterium nucleatum
Genomic DNA was extracted from colorectal carcinoma tissue and adjacent non-tumor tissue in whole-tissue sections of FFPE tissue blocks using QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA). Custom TaqMan primer/probe sets (Applied Biosystems, San Diego, CA) for the 16S ribosomal RNA gene DNA sequence of Fusobacterium nucleatum and for the reference gene, SLCO2A1 were used as previously described.24 (link) The primer/probe set for Fusobacterium nucleatum was designed to target the nusG gene of Fusobacterium nucleatum, and it has been demonstrated that the amount of Fusobacterium nucleatum measured by the quantitative PCR assay highly correlates with that measured by using transcriptome sequencing data (Pearson’s r = 0.97).24 (link) Each reaction contained 80 ng of genomic DNA and was assayed in 20 µL reactions containing 1× final concentration TaqMan Environmental Master Mix 2.0 (Applied Biosystems, San Diego, CA) and each TaqMan Gene Expression Assay (Applied Biosystems, San Diego, CA), in a 96-well optical PCR plate. Amplification and detection of DNA was performed with the StepOnePlus Real-Time PCR Systems (Applied Biosystems, San Diego, CA) using the following reaction conditions: 10 min at 95°C and 45 cycles of 15 sec at 95°C and 1 min at 60°C. The primer and probe sequences for each TaqMan Gene Expression Assay were as follows: Fusobacterium nucleatum forward primer, 5’-CAACCATTACTTTAACTCTACCATGTTCA-3’; Fusobacterium nucleatum reverse primer, 5’-GTTGACTTTACAGAAGGAGATTATGTAAAAATC-3’; Fusobacterium nucleatum FAM probe, 5’-GTTGACTTTACAGAAGGAGATTA-3’; SLCO2A1 forward primer, 5’-ATCCCCAAAGCACCTGGTTT-3’; SLCO2A1 reverse primer, 5’-AGAGGCCAAGATAGTCCTGGTAA-3’; SLCO2A1 VIC probe, 5’-CCATCCATGTCCTCATCTC-3’. In colorectal carcinoma cases with detectable Fusobacterium nucleatum, the cycle threshold (Ct) values in the quantitative PCR for Fusobacterium nucleatum and SLCO2A1 decreased linearly with the amount of input DNA (in a log scale) from the same specimen (r2 > 0.99; Figure 1A). The inter-assay coefficient of variation of Ct values from the same specimen in five different batches was 1% or less for all targets in our validation study using seven colorectal carcinomas (eTable 1 in the Supplement).
Each specimen was analyzed in duplicate for each target in a single batch, and we used the average of the two Ct values for each target. Spearman’s rank-correlation coefficients between the two Ct values (in duplicated runs) in each of cases with detectable target amplification in the quantitative PCR assays for Fusobacterium nucleatum (n = 76) and SLCO2A1 (n = 598) were 0.95 and 0.92, respectively. The amount of Fusobacterium nucleatum in each specimen was calculated as a relative unitless value normalized with SLCO2A1 using the 2−ΔCt method (where ΔCt = “the average Ct value of Fusobacterium nucleatum” - “the average Ct value of SLCO2A1”) as previously described.32 (link)
Mima K., Sukawa Y., Nishihara R., Qian Z.R., Yamauchi M., Inamura K., Kim S.A., Masuda A., Nowak J.A., Nosho K., Kostic A.D., Giannakis M., Watanabe H., Bullman S., Milner D.A., Harris C.C., Giovannucci E., Garraway L.A., Freeman G.J., Dranoff G., Chan A.T., Garrett W.S., Huttenhower C., Fuchs C.S, & Ogino S. (2015). Fusobacterium nucleatum and T-cells in Colorectal Carcinoma. JAMA oncology, 1(5), 653-661.
Publication 2015
Adjustment Disorders Biological Assay Colorectal Carcinoma Dietary Supplements Fusobacterium nucleatum Gene Expression Genes Genome Neoplasms Oligonucleotide Primers RNA, Ribosomal, 16S Tissues
2
Quantitative PCR for Total Bacterial Load
Total bacterial load was analyzed by quantitative PCR (7500 Fast Real-Time PCR system, Applied Biosystems, catalogue 4351107, Foster City, CA) using universal primers-probe set targeting the 16S rDNA gene, as described previously [6] (link). All samples were processed in quadruplicates.
CT values were related to the standard curve ranging from 0.1 pg/µl to 1 ng/µl of bacterial DNA. The reference DNA for the q-PCR standard curve was purified from 800 µl human saliva spiked with 104-bacteria of 6 orals strains, Streptococcus mutans, Fusobacterium nucleatum, Porphyromonas gingivalis, Porphyromonas catoniae, Propionibacterium propionicum, Tannerella forsythia. Total bacterial DNA was quantified by nanodrop and diluted to fit targeted concentrations. The reported qPCR results adhere to the MIQE standards for reporting qPCR data (Methods S2).
Biesbroek G., Sanders E.A., Roeselers G., Wang X., Caspers M.P., Trzciński K., Bogaert D, & Keijser B.J. (2012). Deep Sequencing Analyses of Low Density Microbial Communities: Working at the Boundary of Accurate Microbiota Detection. PLoS ONE, 7(3), e32942.
Publication 2012
Bacteria DNA, Bacterial DNA, Ribosomal Fusobacterium nucleatum Genes Homo sapiens Oligonucleotide Primers Porphyromonas catoniae Porphyromonas gingivalis Pseudopropionibacterium propionicum Saliva Strains Streptococcus mutans Tannerella forsythia
3
Evaluation of Chlamydia LAMP Assays
C. psittaci LAMP assay was evaluated using: (1) 12 DNA samples extracted from previously characterised C. psittaci isolates (10 human, two parrot and one equine) (Table S1); (2) DNA extracted from 21 placental, foetal, nasal, lung and rectal swabs, and 1 each placental and foetal tissue sample taken from 20 equine hosts; and (3) three pigeon liver DNA extracts (Table S2). All samples were collected and submitted as part of routine diagnostic testing by field or district veterinarians to the State Veterinary Diagnostic Laboratory (SVDL), Elizabeth Macarthur Agricultural Institute (EMAI), Menangle, NSW, Australia, and as such do not require special animal ethics approval. DNA extracts from these samples were kindly provided by Dr. Cheryl Jenkins, and Dr. James Branley. The use of these swabs was considered by the University of The Sunshine Coast (USC) Animal Ethics Committee and the need for further ethics consideration was waived under exemption AN/E/17/19.
C. pecorum LAMP was evaluated using a: (1) 18 DNA samples extracted from previously characterised koala (n = 7), sheep (n = 4), cattle (n = 4) and pig C. pecorum (n = 3) cultures (Table S1); (2) 16 sheep and 13 cattle ocular, rectal, and tissue swab DNA samples; and (3) 34 ocular and urogenital (UGT) koala swab DNA samples (Table S3), all available in our collection. The use of these swabs, also collected by qualified veterinarians as a part of routine diagnostic testing, was considered and approved for exemption by the University of The Sunshine Coast (USC) Animal Ethics Committee (AN/E/14/01 and AN/E/14/31).
We also evaluated the specificity of the assays against DNA samples extracted from previously characterised (i) chlamydial isolates (koala C. pneumoniae LPColN, C. abortus S26/3, C. suis S45, C. trachomatis serovar D, C. murridarum Nigg, C. caviae GPIC) and uncultured Chlamydiales (Fritschea spp.); (ii) Gram negative Escherichia coli and Prevotella bivia; Gram positive Fusobacterium nucleatum, Staphylococcus epidermidis, S. aureus, Streptococcus spp., and Enterococcus faecalis; and (iii) commercially available human gDNA (Promega, Alexandria, NSW 2015), all available in our laboratory (Table S1).
In order to evaluate rapid swab processing, 18 ocular, cloacal and UGT (14 dry and four RNA-Later) clinical swabs taken from 14 koalas with presumptive chlamydiosis were used for testing without DNA extraction. Briefly, RNA-Later and dry swabs with added 500 µL TE buffer were vortexed vigorously for 5 min. 300 µL aliquots were then heated to 98 °C for 15 min to lyse DNA, following LAMP testing. The use of these swabs, collected as a part of routine diagnostic testing, is also under Animal Ethics approval exemption (AN/E/14/01). An aliquot of 50 µL of the swab suspension was used for LAMP and qPCR assays, while from the remaining volume of the swab suspension was used for DNA extraction, in order to compare swab suspension and its paired extracted DNA as a template in the assays.
Jelocnik M., Islam M.M., Madden D., Jenkins C., Branley J., Carver S, & Polkinghorne A. (2017). Development and evaluation of rapid novel isothermal amplification assays for important veterinary pathogens: Chlamydia psittaci and Chlamydia pecorum. PeerJ, 5, e3799.
Publication 2017
Animal Ethics Committees Animals Biological Assay Buffers Care, Prenatal Cattle Chlamydia Chlamydiales Columbidae Diagnosis Domestic Sheep Enterobacter Enterococcus faecalis Equus caballus Escherichia coli Fetal Tissue Fetuses, Aborted Fusobacterium nucleatum Homo sapiens LAMP assay Liver Extracts Lung Nose Parrots Phascolarctos cinereus Placenta Pneumonia Prevotella bivia Promega Rectum Staphylococcus aureus Staphylococcus epidermidis Streptococcus Sunlight System, Genitourinary Tissues Veterinarian Vision
4
Quantifying Oral Pathogenic Bacteria
The detection of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), and Treponema denticola in pooled plaque samples was evaluated by real-time qPCR, as described,20 (link),21 (link) using primers specific for the hypervariable segments of the 16S rRNA genes of each bacterium (Table 1). The percentage of the total flora for each species was calculated by dividing the number of target organisms by the total number of bacteria as determined by qPCR using 16S rRNA primers that reacted with all bacterial species. Data were represented using a patient-based assessment.
Ramseier C.A., Kinney J.S., Herr A.E., Braun T., Sugai J.V., Shelburne C.A., Rayburn L.A., Tran H.M., Singh A.K, & Giannobile W.V. (2009). Identification of Pathogen and Host-Response Markers Correlated With Periodontal Disease. Journal of periodontology, 80(3), 436-446.
Publication 2009
Aggregatibacter actinomycetemcomitans Bacteria Campylobacter rectus Dental Plaque Fusobacterium nucleatum Oligonucleotide Primers Patients Porphyromonas gingivalis Prevotella intermedia Ribosomal RNA Genes RNA, Ribosomal, 16S Tannerella forsythia Treponema denticola
5
Cultivation of Anaerobic Oral Bacteria

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Publication 2017
Arecaceae Bacteria, Anaerobic Bacteroides fragilis Brain Culture Media Cysteine Enterococcus faecalis Escherichia coli Fusobacterium nucleatum Heart Hemin Meat Parvimonas micra Peptostreptococcus anaerobius potassium phosphate, dibasic Prevotella intermedia Strains Tremor Vitamin K1

Most recents protocols related to «Fusobacterium nucleatum»

1
Evaluating Bacterial Growth Competition
Not available on PMC !

Example 9

The staggered inoculation setup is shown in FIG. 3E.

Goal: Determine if growth competition between the inhibitory bacteria and Sm played a role in the inhibition of acid production.

Protocol: The cells of Rothia were grown overnight at 37° C. in shaking aerobic conditions while Fn (Fusobacterium nucleatum) and Va (Veillonella atypica) were grown overnight at 37° C. in anaerobic conditions. Fn was grown in Columbia Broth and Va in BHI. The cell cultures had their optical density measured and were diluted to OD600 0.5 in fresh BHI media. 20 μL of each culture were spotted onto a BHI agar plate with Phenol red at two location. The spots were dried then put into their respective growth conditions overnight. The next day the process was repeated with Sm. The cells of Sm were grown overnight at 37° C. in aerobic +5% CO2 conditions and diluted to OD600 0.5 in fresh BHI media the next day. 20 μL of the diluted Sm was spotted on the plate in two locations: one by itself and one adjacent to one of the spots of the inhibitor bacterial species. The plates were dried and incubated overnight at 37° C. in aerobic +5% CO2 conditions and the pH of the Sm alone and the Sm co-culture colonies (“Co-Sm”) were measured using a flat bottom pH probe.

US11872255B2. Methods and compositions of preventing and treating dental caries (2024-01-16). Forsyth Dental Infirmary for Children [US]. Inventors: Pooja Balani [US], Felicitas Bidlack [US], Xuesong He [US], Megan Pugach-Gordon [US], Wenyuan Shi [US], Jacqueline Starr [US], Daniel Ferrer [US].
Patent 2024
Acids Agar Bacteria Bacteria, Aerobic Cell Culture Techniques Coculture Techniques Exanthema Fusobacterium nucleatum Psychological Inhibition Vaccination Veillonella atypica Vision
2
Pathogenic Bacteria Characterization
S. Typhimurium strain 14028S and strain SL1344, Fusobacterium nucleatum strain ATCC 25586, Enterococcus faecalis strain ATCC 29212, Enteropathogenic Escherichia coli (EPEC) strain E2348/69 and Listeria monocytogenes strain 10403S were used in this study.
Wang D.N., Ni J.J., Li J.H., Gao Y.Q., Ni F.J., Zhang Z.Z., Fang J.Y., Lu J, & Yao Y.F. (2023). Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation. PLOS Pathogens, 19(2), e1011189.
Publication 2023
Enterococcus faecalis Enteropathogenic Escherichia coli Fusobacterium nucleatum Listeria monocytogenes Strains
3
Biofilm Formation Protocols for Clinically Relevant Microbes
The biofilms were developed on 96-well flat-bottomed polystyrene microtitre plates (Eppendorf, Hamburg, Germany) by using different media and growth conditions depending on the species. The OD600 of each inoculum was read and adjusted to reach a final concentration in each well of 1.0 × 105 CFU/mL. More details are given as follows:

Escherichia coli ATCC 25922 was grown in Mueller Hinton Broth (MHB; Oxoid Limited, Hampshire, United Kingdom), and the biofilm was developed in MHB for 24 h of incubation at 37°C in static and aerobic conditions;

Pseudomonas aeruginosa ATCC 27853 growth and biofilm formation were conducted in Luria Bertani Broth (LB; Oxoid Limited, Hampshire, United Kingdom) for 24 h of incubation at 37°C in static and aerobic conditions;

Streptococcus mutans UA 159 was grown in Brain Heart Infusion (BHI; Oxoid Limited, Hampshire, United Kingdom), and the biofilm was developed in BHI + 1% of sucrose (BHIS) for 24 h of incubation at 37°C in static and anaerobic conditions;

Staphylococcus aureus ATCC 29213 was grown in Tryptic Soy Broth (TSB; Oxoid Limited, Hampshire, United Kingdom), and the biofilm was developed in TSB + 1% of glucose (TSBG) for 24 h of incubation at 37°C in static and aerobic conditions;

Fusobacterium nucleatum ATCC 25586 growth and biofilm formation were conducted in BHI for 48 h of incubation at 37°C in static and anaerobic conditions.

Vitale I., Spano M., Puca V., Carradori S., Cesa S., Marinacci B., Sisto F., Roos S., Grompone G, & Grande R. (2023). Antibiofilm activity and NMR-based metabolomic characterization of cell-free supernatant of Limosilactobacillus reuteri DSM 17938. Frontiers in Microbiology, 14, 1128275.
Publication 2023
Bacteria, Aerobic Biofilms Brain Escherichia coli Fusobacterium nucleatum Glucose Growth Disorders Heart Polystyrenes Pseudomonas aeruginosa Staphylococcus aureus Streptococcus mutans Sucrose tryptic soy broth
4
Antimicrobial Efficacy of Alocasia paeoniifolius
Standard strains of Porphyromonas gingivalis (ATCC 33227), Prevotella intermedia (JCM 11150), and Fusobacterium nucleatum (ATCC 25886) were obtained from our Central Research Laboratory, and a commercially available pure-based tetracycline hydrochloride® was used.
A. paeoniifolius tubers were procured locally, washed thoroughly, sliced into small pieces, shade dried, and milled to powder form using a mechanical grinder. Then, the obtained powder was stored in airtight bottles. The aqueous and ethanolic extracts of A. paeoniifolius were prepared using the Soxhlet extraction method.[4 (link)]
The serial broth dilution method was carried out to check the minimum inhibitory concentrations of extracts of A. paeoniifolius and tetracycline hydrochloride. Stock solutions for our study were constituted by mixing 200 mg of prepared aqueous and ethanolic extracts in 1 ml of distilled water, respectively. To study the minimal inhibitory concentrations (MIC) of aqueous extract of A. paeoniifolius, a set of 12 sterile vials were labeled serially from 1 to 12 and placed on a rack. Tube number 1 consisted of 400 ml of working dilution of aqueous extract of A. paeoniifolius (stock solution). 200 ml of thioglycolate broth was added in all tubes numbered from 2 to 12. Followed by this, 10 ml of bacterial suspension was added from tube number 1 to 10 and tube number 12. To tube number 11, no organism was added. From tube number 1, 200 ml of A. paeoniifolius extract was transferred to tube number 2 and mixed well. Now, from the mixed solution in the tube number 2, 200 ml of solution was transferred to the tube number 3 and was mixed well. This was continued till tube number 11 and at last 200 ml of the mixture was discarded from tube number 11. Tube number 11 consisted of broth and extract but no organism, and this was considered as broth control (to ascertain sterility check). Tube number 12 consisted of broth and organism but no extract and was considered as organism control (to check organism growth). By following this serial dilution, the concentrations of the aqueous extract of A. paeoniifolius achieved were 100, 50, 25, 12.5, 6.25, 3.1, 1.6, 0.8, 0.4, and 0.2 mg/ml, respectively. The tubes were then incubated for 48 h at 37°C. After the incubation, a visual inspection was carried out to determine the MIC values. Turbidity in the MIC tube indicated growth of the bacteria implying that the bacteria were resistant to the aqueous extract of A. paeoniifolius. The same procedure was carried out for ethanolic extract of A. paeoniifolius and tetracycline hydrochloride. Tetracycline hydrochloride acts as a standard control group in the present study.
From the MIC dilution tubes, the tubes which were found sensitive to MIC were plated and the colony count was noted after incubating it for 24 h. MBC was done to see whether there was bactericidal or bacteriostatic effect of the extracts and tetracycline hydrochloride against the selected organisms. If there was no growth, it was considered to have bactericidal effect. If there was growth, it was concluded to be bacteriostatic.
Nayak A., Sowmya B.R., Gandla H., Kottrashetti V., Ingalagi P, & Srinivas V.S. (2023). Determination and comparison of antimicrobial activity of aqueous and ethanolic extracts of Amorphophallus paeoniifolius on periodontal pathogens: An in vitro study. Journal of Indian Society of Periodontology, 27(1), 40-44.
Publication 2023
Bacteria Ethanol Fusobacterium nucleatum Minimum Inhibitory Concentration Plant Tubers Porphyromonas gingivalis Powder Prevotella intermedia SERPINA3 protein, human Sterility, Reproductive Strains Technique, Dilution Tetracycline Hydrochloride Thioglycolates
5
Characterization of Fusobacterium nucleatum
Fusobacterium nucleatum ATCC25586 (Fn) was purchased from Beijing Beina Chuanglian Institute of Biotechnology. The strain was inoculated in thioglycolate liquid medium and incubated anaerobically at 37 °C (80% N2, 10% CO2, 10% H2). The growth cycle of Fn was determined by Bioscreen C and the structure of the bacterium was observed by scanning electron microscopy and transmission electron microscopy.
Wu X., Xu J., Yang X., Wang D, & Xu X. (2023). Integrating Transcriptomics and Metabolomics to Explore the Novel Pathway of Fusobacterium nucleatum Invading Colon Cancer Cells. Pathogens, 12(2), 201.
Publication 2023
Bacterial Structures Fusobacterium nucleatum Scanning Electron Microscopy Strains Thioglycolates Transmission Electron Microscopy

Top products related to «Fusobacterium nucleatum»

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Hemin by Merck Group
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Hemin is a laboratory product that serves as a source of heme, the iron-containing cofactor found in various hemoproteins. It is commonly used in biochemical and cell culture applications for its role in heme-dependent processes.
2
Fusobacterium nucleatum by American Type Culture Collection
Sourced in United States
Fusobacterium nucleatum is a fastidious, anaerobic, Gram-negative bacterium. It is a common inhabitant of the human oral cavity and is associated with periodontal diseases. This product can be used for research purposes, such as studying the biology and pathogenesis of this organism.
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Menadione by Merck Group
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Menadione is a synthetic form of vitamin K. It is a colorless crystalline solid that is used as a dietary supplement and in the production of various pharmaceutical and industrial products. Menadione serves as a precursor for the production of vitamin K-dependent proteins, which are involved in blood clotting and other physiological processes.
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Fusobacterium nucleatum atcc 25586 by American Type Culture Collection
Sourced in United States
Fusobacterium nucleatum ATCC 25586 is a bacterial strain available from the American Type Culture Collection. It is an obligately anaerobic, non-spore-forming, Gram-negative, pleomorphic bacterium.
5
Porphyromonas gingivalis by American Type Culture Collection
Sourced in United States
Porphyromonas gingivalis is a Gram-negative, anaerobic bacterium. It is a species of the genus Porphyromonas and is commonly found in the human oral cavity.
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Yeast extract by Thermo Fisher Scientific
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Yeast extract is a light-colored, water-soluble powder that is derived from the autolysis of baker's yeast. It serves as a nutrient source, providing a complex mixture of amino acids, vitamins, and other growth factors to support the cultivation of microbial cultures in various scientific and industrial applications.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Staphylococcus aureus by American Type Culture Collection
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Staphylococcus aureus is a bacterial strain available in the American Type Culture Collection (ATCC) product portfolio. It is a Gram-positive, spherical-shaped bacterium commonly found in the human nasal passages and on the skin. This strain is widely used in research and laboratory settings for various applications.
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Streptococcus mutans by American Type Culture Collection
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Streptococcus mutans is a species of bacteria commonly found in the human oral cavity. It is a Gram-positive, coccus-shaped bacterium that is known for its ability to produce lactic acid, which can contribute to the development of dental cavities. This lab equipment product provides a pure culture of Streptococcus mutans for research and study purposes.
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Bhi broth by BD
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BHI broth is a general-purpose microbiological growth medium. It is composed of a mixture of nutrients designed to support the growth of a wide range of microorganisms, including bacteria, yeast, and fungi. The broth provides essential nutrients and growth factors required for microbial proliferation.

More about "Fusobacterium nucleatum"

Fusobacterium nucleatum is an anaerobic, gram-negative bacterium commonly found in the human oral cavity.
It is associated with various oral and systemic diseases, including periodontal disease, colorectal cancer, and adverse pregnancy outcomes.
This versatile pathogen, also known as F. nucleatum or FN, utilizes a range of virulence factors to adhere to host cells, evade immune responses, and promote inflammation.
Hemin and Menadione are important growth factors required by F. nucleatum, which is often cultured in a nutrient-rich medium like BHI (Brain Heart Infusion) broth supplemented with yeast extract and FBS (Fetal Bovine Serum).
F. nucleatum is known to interact with other oral bacteria, such as Porphyromonas gingivalis, Staphylococcus aureus, and Streptococcus mutans, contributing to the complex microbial dynamics in the oral cavity.
Exploring the power of PubCompare.ai's AI-powered platform can enhance research reproducibility and accuracy in the study of F. nucleatum.
This innovative tool helps users locate the best protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the most reliable and effective approaches.
Experience seamless research with PubCompare.ai's intuitive interface and cutting-edged technology.