Log In Sign up
The largest database of trusted experimental protocols
> Living Beings > Bacterium > Lactobacillus plantarum

Lactobacillus plantarum

Lactobacillus plantarum is a Gram-positive, rod-shaped bacterium commonly found in the human gastrointestinal tract, fermented foods, and various other environments.
This probiotic species is known for its ability to produce lactic acid, which contributes to its potential health benefits.
L. plantarum has been studied for its role in maintaining a healthy gut microbiome, modulating immune responses, and potentially aiding in the prevention or management of certain conditions.
Researchers can optimize their investigations of this versatile bacterium using PubCompare.ai, an AI-driven tool that helps locate the best protocols from literature, preprints, and patents.
The protocol comparison feature allows scientists to identify the most reproducible and accurat methods, ensuring the succes of their experiments and advancing the field of L. plantarum research.

Most cited protocols related to «Lactobacillus plantarum»

1
Automated Metabolic Network Reconstruction using Orthology
We used three manually curated metabolic networks, that of Escherichia coli K12 [22 (link)], Lactobacillus plantarum WCFS1 [B. Teusink et al., manuscript in preparation, see also [17]] and Bacillus subtilis subsp. subtilis str. 168 [33 ], as a source to predict automatically a metabolic network for Lactococcus lactis IL1403. The developed method is called AUTOGRAPH (AUtomatic Transfer by Orthology of Gene Reaction Associations for Pathway Heuristics, see Figure 1) and is outlined in detail below. The curated networks were initially constructed with Genomatica's Simpheny™ software for constraint-based modeling purposes, and were retrieved as flat-files containing gene-protein-reaction associations [42 ].
A reference metabolic network of L. lactis IL1403 has been used to evaluate our method (discussed below). This network was also constructed for constraint-based modeling purposes and was retrieved from the authors as a flat-file, containing gene-reaction associations. Throughout the article we will refer to this published reference network as the Oliveira network [31 (link)].
To compare the automatic reconstruction of L. lactis IL1403 metabolic network by Pathologic with that of our method, we used the Genbank NCBI annotation file of L. lactis IL1403 as input for the Pathologic software [27 (link),43 ]. In addition, the same Genbank file together with two manually curated networks from the BioCyc collection (i.e. EcoCyc [16 (link)] and LacplantCyc [17 (link)]) were used as inputs for our method (discussed below).
Notebaart R.A., van Enckevort F.H., Francke C., Siezen R.J, & Teusink B. (2006). Accelerating the reconstruction of genome-scale metabolic networks. BMC Bioinformatics, 7, 296.
Full text: Click here
Publication 2006
Bacillus subtilis subsp. subtilis Escherichia coli K12 Gene Products, Protein Genes Gene Transfer, Horizontal Lactobacillus plantarum Lactococcus lactis Metabolic Networks Milk, Cow's Reconstructive Surgical Procedures
2
Culturing and Inactivating Bacterial Strains
Lactobacillus plantarum NCIMB 8826 (ATCC BAA-793) and Lactobacillus reuteri F275 (ATCC 23272) were cultured overnight at 37°C in Difco Lactobacilli MRS Broth (BD Biosciences, Sparks, MD). Listeria innocua Seeliger (ATCC BAA-680) cultures were generated by overnight growth at 37°C in Sheep Brain Heart infusion medium (Thomas Scientific). Serial dilutions were plated and counted to correlate the number of colony-forming units per milliliter (cfu/mL) with the optical density at 600 nm (OD600). Bacterial cultures were harvested by centrifugation (5 min, 1500 rpm), washed with phosphate-buffered saline (PBS), and suspended in PBS with 1% bovine serum albumin (BSA) just prior to inoculation. Inactivated bacteria were prepared by washing in PBS and heating to 95°C for 30 min prior washing in PBS and resuspension in PBS with 1% BSA; inactivation was confirmed by overnight culture in appropriate culture broths.
Gabryszewski S.J., Bachar O., Dyer K.D., Percopo C.M., Killoran K.E., Domachowske J.B, & Rosenberg H.F. (2010). Lactobacillus-mediated priming of the respiratory mucosa protects against lethal pneumovirus infection. Journal of Immunology (Baltimore, Md. : 1950), 186(2), 1151-1161.
Publication 2010
Bacteria Brain Centrifugation Domestic Sheep Heart Lactobacillus Lactobacillus plantarum Lactobacillus reuteri Listeria innocua Phosphates Saline Solution Serum Albumin, Bovine Technique, Dilution Vaccination
3
Quantification of Gut Microbiome Composition
The bacterial counts was estimated from the slope of the standard curve as described earlier [43 (link)], generated by using the following standard strains: Ruminococcus productus ATCC 27340T (for the Clostridium coccoides group), Faecalibacterium prausnitzii ATCC 27768T (for the Clostridium leptum group), Bacteroides vulgatus ATCC 8482T (for the Bacteroides fragilis group), Bifidobacterium longum subsp. longum ATCC 15707T (for the Genus Bifidobacterium), Collinsella aerofaciens ATCC 25986T (for the Atopobium cluster), Prevotella melaninogenica ATCC 25845T (for the Genus Prevotella), Bacteroides caccae ATCC 43185T (for Bacteroides caccae), Bacteroides eggerthii ATCC 27754T (for Bacteroides eggerthii), Bacteroides fragilis ATCC 25285T (for Bacteroides fragilis), Bacteroides ovatus ATCC 8483T (for Bacteroides ovatus), Bacteroides thetaiotaomicron ATCC 29148T (for Bacteroides thetaiotaomicron), Bacteroides uniformis ATCC 8492T (for Bacteroides uniformis), Bacteroides vulgatus ATCC 8482T (for Bacteroides vulgatus), Bifidobacterium adolescentis ATCC 15703T (for the Bifidobacterium adolescentis group), Bifidobacterium animalis subsp. lactis DSM 10140T (for Bifidobacterium animalis subsp. lactis), Bifidobacterium bifidum ATCC 29521T (for Bifidobacterium bifidum), Bifidobacterium breve ATCC 15700T (for Bifidobacterium breve), Bifidobacterium pseudocatenulatum JCM 1200T (for the Bifidobacterium catenulatum group), Bifidobacterium dentium ATCC 27534T (for Bifidobacterium dentium), Bifidobacterium longum subsp. infantis ATCC 15697T (for Bifidobacterium longum subsp. infantis), Bifidobacterium longum subsp. longum ATCC 15707T (for Bifidobacterium longum subsp. longum), Clostridium perfringens JCM 1290T (for Clostridium perfringens), Escherichia coli ATCC 11775T (for the Family Enterobacteriaceae), Enterococcus faecalis ATCC 19433T (for the Genus Enterococcus), Staphylococcus aureus ATCC 12600T (for the Genus Staphylococcus), Lactobacillus fermentum ATCC 14931T (for Lactobacillus fermentum), Lactococcus lactis subsp. lactis JCM 5805T (for the Lactococcus lactis subgroup), Lactobacillus casei ATCC 334T (for the Lactobacillus casei subgroup), Lactobacillus gasseri DSM 20243T (for the Lactobacillus gasseri subgroup), Lactobacillus plantarum ATCC 14917T (for the Lactobacillus plantarum subgroup), Lactobacillus sakei subsp. sakei JCM 1157T (for the Lactobacillus sakei subgroup), Lactobacillus reuteri JCM 1112T (for the Lactobacillus reuteri subgroup), and Lactobacillus ruminis JCM 1152T (for the Lactobacillus ruminis subgroup).
Martin R., Makino H., Cetinyurek Yavuz A., Ben-Amor K., Roelofs M., Ishikawa E., Kubota H., Swinkels S., Sakai T., Oishi K., Kushiro A, & Knol J. (2016). Early-Life Events, Including Mode of Delivery and Type of Feeding, Siblings and Gender, Shape the Developing Gut Microbiota. PLoS ONE, 11(6), e0158498.
Full text: Click here
Publication 2016
Bacteroides caccae Bacteroides eggerthii Bacteroides fragilis Bacteroides ovatus Bacteroides thetaiotaomicron Bacteroides uniformis Bacteroides vulgatus Bifidobacterium Bifidobacterium adolescentis Bifidobacterium animalis Bifidobacterium bifidum Bifidobacterium breve Bifidobacterium catenulatum Bifidobacterium dentium Bifidobacterium longum subsp. longum Bifidobacterium longum subspecies infantis Bifidobacterium pseudocatenulatum Blautia coccoides Blautia producta Clostridium Clostridium perfringens Collinsella aerofaciens Counts, Bacterial Enterobacteriaceae Enterococcus Enterococcus faecalis Escherichia coli Faecalibacterium prausnitzii Lacticaseibacillus casei Lactobacillus gasseri Lactobacillus plantarum Lactobacillus reuteri Lactobacillus ruminis Lactobacillus sakei Lactobacillus sakei subsp. sakei Lactococcus lactis Lactococcus lactis subsp. lactis Limosilactobacillus fermentum Prevotella Prevotella melaninogenica Staphylococcus Staphylococcus aureus Strains
4
Comparative Microbial Community Analysis
A defined microbial community was prepared in order to compare Illumina library sequencing to traditional Sanger sequencing. To prepare this community, genomic DNA from the following 12 bacterial strains was added in equivalent amounts with the exception of Staphylococcus, for which threefold more DNA was added: Pseudomonas aeruginosa (ATCC 10145), Escherichia coli (ATCC 11303), Klebsiella pneumoniae (Macdonald Campus of McGill culture collection), Alicaligenes faecalis (ATCC 8750), Enterobacter aerogenes (ATCC 13048), Lactobacillus plantarum (ATCC 8014), Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 19433), Citrobacter freundii (ATCC 8090), Proteus vulgaris (ATCC 6380), Clostridia sporogenes (ATCC 19404), Staphylococcus epidermidis (ATCC 12228). The V3 region of the 16S rRNA gene was amplified as described above, in duplicate using two different barcodes, in order to evaluate library reproducibility. These additional two libraries were sequenced as part of a separate Illumina run containing additional environmental samples. Near full-length 16S rRNA genes from the same pool of DNA were amplified using 27F/1492R primers as described above and inserted into the TOPO cloning system (Invitrogen, Canada) then used to transform E. coli, as per kit instructions. Ninety-six positive clones were chosen and sequenced with conventional Sanger sequencing. All sequences were classified using the RDP classifier with the same conditions as below.
Stearns J.C., Lynch M.D., Senadheera D.B., Tenenbaum H.C., Goldberg M.B., Cvitkovitch D.G., Croitoru K., Moreno-Hagelsieb G, & Neufeld J.D. (2011). Bacterial biogeography of the human digestive tract. Scientific Reports, 1, 170.
Publication 2011
Bacillus subtilis Citrobacter freundii Clone Cells Clostridium sporogenes DNA, Bacterial DNA Library Enterobacter aerogenes Enterococcus faecalis Escherichia coli Genome Klebsiella pneumoniae Lactobacillus plantarum Microbial Community Oligonucleotide Primers Proteus vulgaris Pseudomonas aeruginosa Ribosomal RNA Genes Staphylococcus Staphylococcus epidermidis Strains Topotecan
5
Growth of Lactiplantibacillus plantarum UTNGt2
Lactiplantibacillus (formally Lactobacillus) plantarum strain UTNGt2 was grown in MRS medium (Merck, Darmstadt, Germany) at 37 °C under static aerobic conditions. The strain was previously identified based on 16S rDNA sequencing and was registered in 2018 (November) with GenBank accession no. KY041688.1.
Tenea G.N, & Ortega C. (2021). Genome Characterization of Lactiplantibacillus plantarum Strain UTNGt2 Originated from Theobroma grandiflorum (White Cacao) of Ecuadorian Amazon: Antimicrobial Peptides from Safety to Potential Applications. Antibiotics, 10(4), 383.
Full text: Click here
Publication 2021
Bacteria, Aerobic DNA, Ribosomal Lactobacillus plantarum Strains

Most recents protocols related to «Lactobacillus plantarum»

1
Probiotic Supplement Formulation and Preparation
Not available on PMC !

Example 1

NAME OF COMPONENTmg/sachet
Probiotic Material:
Lactobacillus helveticus150 billion CFU/g73.333
Rosell 52
Bifidobacterium longum 50 billion CFU/g20.000
R175
Lactobacillus plantarum150 billion CFU/g20.000
Rosell 1012
Carrier material:
Magnesium oxide41.446
Magnesium gluconate341.297
Potassium citrate138.290
Zinc gluconate111.111
Glutathione20.000
Lactoferrin11.364
Copper citrate2.834
Inulin500.000
Fructose1291.125
Additional (optional) excipients
Sucralose4.000
Acesulfame K12.000
Flavouring150.000
Aerosil 20040.000
Colouring: E1242.200
Colouring: E1021.000
Anhydrous citric acid220.000

The formulation described above is prepared as follows: Lactobacillus Plantarum, Lactobacillus helveticus, Bifidobacterium longum, are mixed with inulin and blended at 32 rpm for approximately 10 min. Thereafter, fructose, magnesium gluconate, zinc gluconate, citric acid, flavor, potassium citrate, magnesium oxide, silicon dioxide, glutathione, potassium acesulfame, lactoferrine, and sucralose are added to the mixture and blended at 32 rpm for another 10 min.

US11878040B2. Compositions comprising probiotic and prebiotic components and mineral salts, with lactoferrin (2024-01-23). GlaxoSmithKline Consumer Healthcare S.R.L. [RO]. Inventors: Valeria Longoni [IT], Marisa Penci [IT].
Full text: Click here
Patent 2024
acesulfame potassium Aerosil Bifidobacterium longum Citric Acid Citric Acid, Anhydrous Copper Excipients Flavor Enhancers Fructose gluconate Glutathione Inulin Lactobacillus Lactobacillus helveticus Lactobacillus plantarum Lactoferrin Magnesium magnesium gluconate Minerals Oxide, Magnesium Oxides Potassium Citrate Prebiotics Probiotics Salts Silicon Dioxide sucralose zinc gluconate
2
Culturing Lactobacillus plantarum for Inoculation
Lactobacillus plantarum (L. plantarum) was grown in MRS broth medium at 37°C without shaking. After 24 h, the final concentration of the bacterial solution was 2 × 109 CFU/mL, which was inoculated into the new MRS Broth medium at the rate of 2% for extended culture. After 24 h of culture in a shaker, centrifuged at 4000 rpm for 10 min, take the bacterial mud for use.
Lv W., Ma Y., Zhang Y., Wang T., Huang J., He S., Du H, & Guo S. (2023). Effects of Lactobacillus plantarum fermented Shenling Baizhu San on gut microbiota, antioxidant capacity, and intestinal barrier function of yellow-plumed broilers. Frontiers in Veterinary Science, 10, 1103023.
Full text: Click here
Publication 2023
Bacteria Lactobacillus plantarum
3
Probiotic Supplementation in Poultry Feed Formulations

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Acids Agar Biopharmaceuticals Biotin Calcium, Dietary Cholecalciferol Cobalamins Copper Corns Diet Dietary Services Ethylenediamines Folic Acid Glucose Iron Lactobacillus Lactobacillus plantarum Lymphoid Progenitor Cells Manganese menadione nicotinamide bisulfite Minerals Niacin Potato Probiotics Proteins Pyridoxine Hydrochloride Riboflavin Saccharomyces cerevisiae Selenium Soybean Flour Strains Thiamine Mononitrate Vitamin A Vitamin E Vitamins Zinc
4
Isolation and Characterization of Probiotic Lactobacillus

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Acids AT2G25170 protein, Arabidopsis Bacteria Caco-2 Cells Cardiac Arrest Immunomodulation In Vitro Techniques Lactobacillales Lactobacillus Lactobacillus plantarum Pathogenicity Saline Solution Salts, Bile Technique, Dilution
5
Enhancing Hatchability with Lactobacillus Injection

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Amniotic Fluid Biological Assay BLOOD Cerebrovascular Accident Chickens Culture Media Disinfection DNA Replication Eggs Egg Shell Egg White Embryo Fertility Fowls, Domestic Freezing Lactobacillus Lactobacillus plantarum physiology Saline Solution Serum Sterility, Reproductive Stomach Syringes Vacuum Veins Zygote

Top products related to «Lactobacillus plantarum»

1
Mrs broth by Thermo Fisher Scientific
Sourced in United Kingdom, Italy, United States, Australia, Spain, Canada, Sweden, Belgium, Norway, Germany
MRS broth is a microbiological medium used for the selective isolation and cultivation of lactobacilli. It provides the necessary nutrients and growth factors for the optimal growth of lactobacilli species. The composition of the broth includes various peptones, yeast extract, glucose, and specific salts.
2
Mrs broth by Merck Group
Sourced in Germany, United States, United Kingdom, Singapore, Japan, Spain
MRS broth is a culture medium used for the isolation and cultivation of lactic acid bacteria, particularly Lactobacillus species. It provides the necessary nutrients and growth factors required by these microorganisms. The formulation of MRS broth is based on the de Man, Rogosa, and Sharpe (MRS) medium.
3
Mrs broth by BD
Sourced in United States, Japan
MRS broth is a microbiological culture medium used for the isolation and cultivation of lactic acid bacteria. It provides the necessary nutrients and growth factors for the proliferation of these bacteria in a laboratory setting.
4
Mrs medium by Merck Group
Sourced in Germany, United States, United Kingdom
MRS medium is a laboratory culture medium commonly used for the cultivation and isolation of lactic acid bacteria. It is formulated to support the growth of a wide range of Lactobacillus and other lactic acid-producing microorganisms. The medium provides essential nutrients and growth factors required by these bacteria.
5
Lactobacillus plantarum by American Type Culture Collection
Sourced in United States
Lactobacillus plantarum is a bacterial strain available for laboratory use. It is a gram-positive, facultatively anaerobic, non-spore-forming rod-shaped bacterium. Lactobacillus plantarum is commonly used in research and industrial applications.
6
Mrs agar by Merck Group
Sourced in Germany, United States, Italy, United Kingdom, Hungary, Poland
MRS agar is a laboratory culture medium used for the selective isolation and enumeration of lactic acid bacteria. It is designed to support the growth of organisms such as Lactobacillus, Pediococcus, and Leuconostoc species. MRS agar provides the necessary nutrients and growth factors required by these microorganisms.
7
Glycerol by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, France, India, Spain, Canada, Switzerland, China, Brazil, Australia, Japan, Ireland, Indonesia, Hungary, Malaysia, Sao Tome and Principe, Sweden, Singapore, Macao, Belgium, Mexico, Romania, Netherlands, Poland, Israel, Portugal, Colombia, New Zealand, Pakistan, Denmark
Glycerol is a colorless, odorless, and viscous liquid used in various laboratory applications. It is a basic chemical compound with the molecular formula C₃H₈O₃. Glycerol is commonly used as a solvent, humectant, and stabilizer in many laboratory procedures.
8
Mrs agar by Thermo Fisher Scientific
Sourced in United Kingdom, United States, Germany, Italy, China, Spain
MRS agar is a growth medium used for the isolation and cultivation of lactic acid bacteria, particularly Lactobacillus species. It provides essential nutrients and growth factors required by these microorganisms. The formulation is based on de Man, Rogosa, and Sharpe's (MRS) original recipe.
9
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
10
Lactobacilli mrs broth by BD
Sourced in United States
Lactobacilli MRS broth is a microbiological culture medium used for the isolation, cultivation, and enumeration of Lactobacillus species. It provides the necessary nutrients and growth factors for the selective growth of Lactobacillus bacteria.

More about "Lactobacillus plantarum"

Lactobacillus plantarum, also known as L. plantarum, is a versatile and well-studied probiotic bacterium that has garnered significant attention in the field of gut health and microbiome research.
This Gram-positive, rod-shaped microorganism is commonly found in the human gastrointestinal tract, fermented foods, and various other environments.
One of the key features of L. plantarum is its ability to produce lactic acid, which contributes to its potential health benefits.
This probiotic species has been extensively studied for its role in maintaining a healthy gut microbiome, modulating immune responses, and potentially aiding in the prevention or management of certain conditions.
Researchers investigating L. plantarum can optimize their studies by utilizing PubCompare.ai, an AI-driven tool that helps locate the best protocols from literature, preprints, and patents.
The protocol comparison feature of PubCompare.ai allows scientists to identify the most reproducible and accurate methods, ensuring the success of their experiments and advancing the field of L. plantarum research.
When working with L. plantarum, researchers often use specialized growth media such as MRS (de Man, Rogosa, and Sharpe) broth or MRS agar.
These media provide the necessary nutrients and growth conditions for the optimal cultivation of this bacterium.
Additionally, glycerol and fetal bovine serum (FBS) may be used in various experimental setups involving L. plantarum.
By leveraging the insights and tools available, researchers can optimize their investigations of this versatile and important probiotic bacterium, Lactobacillus plantarum, and contribute to the advancements in gut microbiome and human health research.