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Australorbis glabratus

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Most cited protocols related to «Australorbis glabratus»

1
Sequencing and Annotation of S. mansoni Genome
Mixed sex cercariae from the Puerto Rico isolate of S. mansoni43 (link), released from infected Biomphalaria glabrata snails, were placed in low-melting agarose plugs and genomic DNA prepared by standard methods. Approximately, six-fold coverage of the nuclear genome was obtained using a whole genome shotgun sequencing approach where libraries of different cloned insert sizes (in plasmid, fosmid and BAC vectors) were randomly sequenced by Sanger technology from either end. Sequence reads were assembled and scaffolds were FISH mapped to individual chromosomes where possible (Supplementary Table 2). The output of several gene prediction algorithms, trained using 409 manually curated gene structures, were integrated into a single set of gene predictions (v4), which were used for subsequent analyses. Data were accessed via GeneDB (http://www.genedb.org) and Artemis was used for subsequent manual annotation and curation of a further 958 genes during subsequent analyses (as described previously44 (link)).
Full methods and all associated references are available in the online version of the paper at www.nature.com/nature.
Further details for additional methods used in this study are provided in Supplementary Information.
Berriman M., Haas B.J., LoVerde P.T., Wilson R.A., Dillon G.P., Cerqueira G.C., Mashiyama S.T., Al-Lazikani B., Andrade L.F., Ashton P.D., Aslett M.A., Bartholomeu D.C., Blandin G., Caffrey C.R., Coghlan A., Coulson R., Day T.A., Delcher A., DeMarco R., Djikeng A., Eyre T., Gamble J.A., Ghedin E., Gu Y., Hertz-Fowler C., Hirai H., Hirai Y., Houston R., Ivens A., Johnston D.A., Lacerda D., Macedo C.D., McVeigh P., Ning Z., Oliveira G., Overington J.P., Parkhill J., Pertea M., Pierce R.J., Protasio A.V., Quail M.A., Rajandream M.A., Rogers J., Sajid M., Salzberg S.L., Stanke M., Tivey A.R., White O., Williams D.L., Wortman J., Wu W., Zamanian M., Zerlotini A., Fraser-Liggett C.M., Barrell B.G, & El-Sayed N.M. (2009). The genome of the blood fluke Schistosoma mansoni. Nature, 460(7253), 352-358.
Publication 2009
Australorbis glabratus Cercaria Chromosomes Cloning Vectors Fishes Genes Genetic Structures Genome Plasmids Sepharose Snails
2
Sequencing and Annotation of S. mansoni Genome
Mixed sex cercariae from the Puerto Rico isolate of S. mansoni43 (link), released from infected Biomphalaria glabrata snails, were placed in low-melting agarose plugs and genomic DNA prepared by standard methods. Approximately, six-fold coverage of the nuclear genome was obtained using a whole genome shotgun sequencing approach where libraries of different cloned insert sizes (in plasmid, fosmid and BAC vectors) were randomly sequenced by Sanger technology from either end. Sequence reads were assembled and scaffolds were FISH mapped to individual chromosomes where possible (Supplementary Table 2). The output of several gene prediction algorithms, trained using 409 manually curated gene structures, were integrated into a single set of gene predictions (v4), which were used for subsequent analyses. Data were accessed via GeneDB (http://www.genedb.org) and Artemis was used for subsequent manual annotation and curation of a further 958 genes during subsequent analyses (as described previously44 (link)).
Full methods and all associated references are available in the online version of the paper at www.nature.com/nature.
Further details for additional methods used in this study are provided in Supplementary Information.
Berriman M., Haas B.J., LoVerde P.T., Wilson R.A., Dillon G.P., Cerqueira G.C., Mashiyama S.T., Al-Lazikani B., Andrade L.F., Ashton P.D., Aslett M.A., Bartholomeu D.C., Blandin G., Caffrey C.R., Coghlan A., Coulson R., Day T.A., Delcher A., DeMarco R., Djikeng A., Eyre T., Gamble J.A., Ghedin E., Gu Y., Hertz-Fowler C., Hirai H., Hirai Y., Houston R., Ivens A., Johnston D.A., Lacerda D., Macedo C.D., McVeigh P., Ning Z., Oliveira G., Overington J.P., Parkhill J., Pertea M., Pierce R.J., Protasio A.V., Quail M.A., Rajandream M.A., Rogers J., Sajid M., Salzberg S.L., Stanke M., Tivey A.R., White O., Williams D.L., Wortman J., Wu W., Zamanian M., Zerlotini A., Fraser-Liggett C.M., Barrell B.G, & El-Sayed N.M. (2009). The genome of the blood fluke Schistosoma mansoni. Nature, 460(7253), 352-358.
Publication 2009
Australorbis glabratus Cercaria Chromosomes Cloning Vectors Fishes Genes Genetic Structures Genome Plasmids Sepharose Snails
3
Biomphalaria glabrata Genome Sequencing
The sequence data that support the findings of this study have been deposited in GenBank with the accession codes SRX005826, -27, -28; SRX008161, -2; SRX648260, -61, -62, -63, -64, -65, -66, -67, -68, -69, -70, -71; SRA480937; SRA480939; SRA480940; SRA480945; TI accessions2091872204-2092480271; 2104228958-2104243968; 2110153721-2118515136; 2181062043-2181066224; 2193113537-2193116528; 2204642410-2204763511; 2204820860-2204852286; 2213009530-2213057324; 2260448774-2260450167. Also see Supplementary Data 1. The assembly and related data are available from VectorBase, https://www.vectorbase.org/organisms/biomphalaria-glabrata. The Biomphalaria glabrata genome project has been deposited at DDBJ/EMBL/GenBank under the accession number APKA00000000.1
Adema C.M., Hillier L.W., Jones C.S., Loker E.S., Knight M., Minx P., Oliveira G., Raghavan N., Shedlock A., do Amaral L.R., Arican-Goktas H.D., Assis J.G., Baba E.H., Baron O.L., Bayne C.J., Bickham-Wright U., Biggar K.K., Blouin M., Bonning B.C., Botka C., Bridger J.M., Buckley K.M., Buddenborg S.K., Lima Caldeira R., Carleton J., Carvalho O.S., Castillo M.G., Chalmers I.W., Christensens M., Clifton S., Cosseau C., Coustau C., Cripps R.M., Cuesta-Astroz Y., Cummins S.F., di Stefano L., Dinguirard N., Duval D., Emrich S., Feschotte C., Feyereisen R., FitzGerald P., Fronick C., Fulton L., Galinier R., Gava S.G., Geusz M., Geyer K.K., Giraldo-Calderón G.I., de Souza Gomes M., Gordy M.A., Gourbal B., Grunau C., Hanington P.C., Hoffmann K.F., Hughes D., Humphries J., Jackson D.J., Jannotti-Passos L.K., de Jesus Jeremias W., Jobling S., Kamel B., Kapusta A., Kaur S., Koene J.M., Kohn A.B., Lawson D., Lawton S.P., Liang D., Limpanont Y., Liu S., Lockyer A.E., Lovato T.L., Ludolf F., Magrini V., McManus D.P., Medina M., Misra M., Mitta G., Mkoji G.M., Montague M.J., Montelongo C., Moroz L.L., Munoz-Torres M.C., Niazi U., Noble L.R., Oliveira F.S., Pais F.S., Papenfuss A.T., Peace R., Pena J.J., Pila E.A., Quelais T., Raney B.J., Rast J.P., Rollinson D., Rosse I.C., Rotgans B., Routledge E.J., Ryan K.M., Scholte L.L., Storey K.B., Swain M., Tennessen J.A., Tomlinson C., Trujillo D.L., Volpi E.V., Walker A.J., Wang T., Wannaporn I., Warren W.C., Wu X.J., Yoshino T.P., Yusuf M., Zhang S.M., Zhao M, & Wilson R.K. (2017). Whole genome analysis of a schistosomiasis-transmitting freshwater snail. Nature Communications, 8, 15451.
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Publication 2017
Australorbis glabratus Genome
4
Genetic Analysis of Schistosoma Isolates from Uganda
Seven ‘population’ isolates were collected from schoolchildren in the Hoima district of Uganda in June 2004; 4 consisted of pooled isolates from 20 children at 4 randomly selected primary schools and the remaining 3 were isolates from individual children. Miracidia were also collected from a fourth infected child, but isolate establishment in the laboratory host failed in this case (Table 2: see Fig. 1 for location of schools). Infected children were identified by positive Kato-Katz smears and all examined children were treated with praziquantel at 40 mg/kg. Miracidia were hatched from faecal samples in bottled spring water. Samples were divided and 48 to 150 miracidia used for the immediate exposure of laboratory bred Biomphalaria glabrata (strain NHM2), at a dose of 6 miracidia per snail. The remaining samples were stored on Whatman FTA® Indicator cards (Whatman plc, Maidstone, UK). The number of snails exposed varied according to the number of miracidia hatched (Table 2). Snails were transported to the UK, where they were maintained in the laboratory in bottled spring water (Sainsburys Supermarkets plc, London, UK) and fed ad libitum on freshly washed lettuce. The laboratory was maintained at 27 °C and subject to a light regime of 12 h light and 12 h darkness, with a 30-min gradual transition at ‘dawn’ and ‘dusk’. Snails were maintained for 35-40 days, the pre-patent period during which larval development to the cercarial stage takes place. Following this time, cercariae from each snail were induced to shed by placing snails in the dark for 24 h and subsequently exposing them, at 10 am to an overhead light source (100 W) for 2 h in vials containing 16 ml of water. Cercariae from the snails representing one isolate were pooled and used to infect 5-10 laboratory mice (strain TO) per isolate at a dose of 220 cercariae per animal by paddling for 0·5 h in 30 ml of infected water. At 7 weeks post-infection, before signs of pathology had occurred, mice were asphyxiated using a rising concentration of carbon dioxide. Adult schistosomes (which varied in number from 24 to several hundred established worms per 4-mouse isolate) were recovered by a modified hepatic perfusion technique (Smithers and Terry, 1965 (link)). Thirty randomly selected adult worms were stored in 100% ethanol until required for genetic analysis, except for isolate Run-1 where only 24 adult worms were recovered and stored. Miracidia from eggs in each of the livers were stimulated to hatch by maceration through a sieve and exposure to a bright light (100 W) source for 30 min in 100 ml of deionized water. Liver samples of each isolate separately, were pooled and 30-50 miracidia per isolate stored for PCR analysis on Whatman FTA® Indicator cards (Whatman plc, Maidstone, UK), with the exception again of isolate Run-1 where a total of only 25 miracidia were successfully hatched.
DNA was extracted from the 30 (or 24 for Run-1) adult worms using phenol chloroform and ethanol precipitation (Davies et al. 1999 (link)) and from 30 field and 30 laboratory miracidial samples (25 for Run-1) as described above. All samples were subject to multiplex PCR for 7 microsatellite loci as described above. A sample size of 30 was selected as this represented the smallest field miracidial sample (isolate Kib-P).
GOWER C.M., SHRIVASTAVA J., LAMBERTON P.H., ROLLINSON D., WEBSTER B.L., EMERY A., KABATEREINE N.B, & WEBSTER J.P. (2006). Development and application of an ethically and epidemiologically advantageous assay for the multi-locus microsatellite analysis of Schistosoma mansoni. Parasitology, 134(Pt 4), 523-536.
Publication 2006
Adult Animals Australorbis glabratus Carbon dioxide Cercaria Child Chloroform Darkness Eggs Ethanol Feces Helminths Infection Lactuca sativa Larva Light Liver Mice, House Mice, Laboratory Multiplex Polymerase Chain Reaction Perfusion Phenols Praziquantel Schistosoma Short Tandem Repeat Snails Strains
5
Schistosoma mansoni Maintenance and Harvest
A Puerto Rican isolate of Schistosoma mansoni was maintained by passage through albino Biomphalaria glabrata snails and infection of 3–5 week-old, female Mesocricetus auratus Golden Syrian hamsters [54 (link), 55 (link)]. Cercariae (infectious larvae) were obtained from infected snails and mechanically transformed into somules as previously described [20 (link), 56 (link), 57 (link)]. To obtain adult schistosomes, hamsters were euthanized 42–45 days post-infection using an intra-peritoneal injection of 50 mg/kg sodium pentobarbital containing 50 U/ml heparin (as an anti-coagulant) in a total of 100 μL PBS. Worms were harvested by reverse perfusion of the hepatic portal system [54 (link), 55 (link), 58 (link)] in RPMI 1640 medium supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin [54 (link), 55 (link)]. Adults were transferred into Basch medium 169 [59 (link)] supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin. In this medium, parasites were washed three times, allowed to stand for 30–60 min in the presence of 2X amphotericin B (fungizone) and then washed another three times in medium minus fungizone prior to phenotypic screening.
Long T., Neitz R.J., Beasley R., Kalyanaraman C., Suzuki B.M., Jacobson M.P., Dissous C., McKerrow J.H., Drewry D.H., Zuercher W.J., Singh R, & Caffrey C.R. (2016). Structure-Bioactivity Relationship for Benzimidazole Thiophene Inhibitors of Polo-Like Kinase 1 (PLK1), a Potential Drug Target in Schistosoma mansoni. PLoS Neglected Tropical Diseases, 10(1), e0004356.
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Publication 2016
Adult Albinism Amphotericin B Australorbis glabratus Cercaria Coagulants Fungizone Hamsters Helminths Heparin Infection Injections, Intraperitoneal Larva Mesocricetus auratus Parasites Penicillins Pentobarbital Sodium Perfusion Phenotype Portal System Puerto Ricans Schistosoma Schistosoma mansoni Snails Streptomycin Woman

Most recents protocols related to «Australorbis glabratus»

1
Schistosoma mansoni lifecycle maintenance
The “Lobato Paraense” snail facility at the René Rachou Institute—FIOCRUZ provided cercariae of S. mansoni (LE strain). The parasite cycle is maintained throughout passages between hamsters (Mesocricetus auratus) and snails (Biomphalaria glabrata).
As previously described, cercariae were mechanically transformed into schistosomula (Milligan and Jolly, 2011 (link)). Schistosomula were cultured in GMEM supplemented with 0.2 μM triiodothyronine; 0.1% glucose; 0.1% lactalbumin; 20 mM HEPES; 0.5% MEM vitamin solution; 5% Schneider’s Insect Medium; and 0.5 μM hypoxanthine, 1 μM hydrocortisone, 1% penicillin/streptomycin, and 2% heat-inactivated FBS.
Hamsters (M. auratus) were infected with cercariae and subjected to perfusion (Pellegrino and Siqueira, 1956 (link)) after 45 days for obtaining adult worms. Males and females were washed, separated manually, and cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with 2% penicillin/streptomycin and 10% heat-inactivated FBS.
Coelho F.S., Gava S.G., Andrade L.F., Geraldo J.A., Tavares N.C., Lunkes F.M., Neves R.H., Machado-Silva J.R., Pierce R.J., Oliveira G, & Mourão M.M. (2023). Schistosoma mansoni coactivator associated arginine methyltransferase 1 (SmCARM1) effect on parasite reproduction. Frontiers in Microbiology, 14, 1079855.
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Publication 2023
2-(beta-(4-hydroxyphenyl)ethylaminomethyl)tetralone Adult Australorbis glabratus Cercaria Culture Media Females Glucose Hamsters Helminths HEPES Hydrocortisone Hypoxanthine Insecta Lactalbumin Liothyronine Males Mesocricetus auratus neuronectin Parasites Penicillins Perfusion Snails Strains Streptomycin Vitamins
2
Gene prediction and annotation for C. bisecta
Ab initio, homology-based and gene expression evidence were combined to predict protein-coding genes in the genome of C. bisecta. Augustus v3.1 was first employed on repeat-masked genome for ab initio gene prediction [106 (link)]. For the homology-based annotation, gene sets from 10 molluscan species (Archivesica marissinica, Biomphalaria glabrata, Crassostrea gigas, Gigantidas platifrons, Lottia gigantea, Lutraria rhynchaena, Modiolus philippinarum, Octopus bimaculoides, Pinctada fucata, and P. canaliculate) were used. These homologous protein sequences were first aligned onto the genome of C. bisecta using Blast v2.2.26 with an e-value cut-off of 1 × 10−5 [107 (link)], and then we linked the alignment hits to candidate gene loci by GenBlastA [108 (link)]. Secondly, genomic sequences of candidate gene regions together with their 2-kb flanking sequences were extracted and used GeneWise v2.2.0 to determine gene models [109 (link)]. Moreover, Stringtie v 1.3.4 was employed to generated gene annotation files on RNA-Seq alignments generated by HISAT v2.1.0 of different tissues (adductor muscle, mantle, foot, and gill) [110 (link), 111 (link)]. Then these files were merged together to predict candidate coding regions open reading frames (ORFs) using Transdecoder v5.5.0 and were aligned to genomes to obtain a gene annotation file with transcript evidence. Finally, these three evidences were integrated using EVM v1.1.1 to obtain a final version of protein-coding genes [112 (link)], and their function were annotated by searching against the following public databases: Swiss-Prot v201709, KEGG v87.0, InterPro v55.0, and TrEMBL v201709. The other 7 species used in gene family analysis were functionally annotated in the same way.
Guo Y., Meng L., Wang M., Zhong Z., Li D., Zhang Y., Li H., Zhang H., Seim I., Li Y., Jiang A., Ji Q., Su X., Chen J., Fan G., Li C, & Liu S. (2023). Hologenome analysis reveals independent evolution to chemosymbiosis by deep-sea bivalves. BMC Biology, 21, 51.
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Publication 2023
Amino Acid Sequence Australorbis glabratus Crassostrea gigas FCER2 protein, human Foot Gene Annotation Gene Expression Gene Products, Protein Genes Genes, vif Genome Gills Homologous Sequences Muscle Tissue Octopus Open Reading Frames Pinctada Protein C Proteins RNA-Seq Tissues
3
Experimental Infection of Hosts for Echinostome Metacercariae
In order to obtain metacercariae experimentally, we attempted to infect laboratory-reared snails [Biomphalaria glabrata (Say, 1818)] and fish (Poecilia reticulata Peters, 1859). These species were used as experimental hosts due to their availability in the laboratory and previous knowledge on the involvement of snails and fish as second intermediate hosts of echinostomes. The behavior of cercariae in the presence of these potential hosts was observed under a stereomicroscope. After 24hs of exposure to cercariae, the snails and fish were necropsied. We also searched for metacercariae in samples of insects, fishes, snails, and tadpoles collected in the same water bodies where snails were found infected.
Metacercariae found in tadpoles collected in the stream where snails were found infected were used for an experimental infection study. We suspected they could be of the same species based on the number of excretory corpuscles. Aiming to obtain adult parasites for taxonomic identification, a sub-sample of 50 metacercariae was orally administered to one specimen of a dexamethasone-immunosuppressed (50 mg/kg) male Swiss mouse. The infected mouse was maintained on a 12/12h light–dark cycle and allowed access to food and water ad libitum. Coproparasitological examinations by the sedimentation technique were conducted daily, starting from seven days post-infection. The mouse was euthanized via barbituric overdose (sodium pentobarbital, injected intraperitoneally) and necropsied for the search of adult parasites 14 days post-infection.
López-Hernández D., Valadão M.C., de Melo A.L., Tkach V.V, & Pinto H.A. (2023). Elucidating the life cycle of opossum parasites: DNA sequences reveal the involvement of planorbid snails as intermediate hosts of Rhopalias spp. (Trematoda: Echinostomatidae) in Brazil. PLOS ONE, 18(3), e0279268.
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Publication 2023
Adult Australorbis glabratus Cercaria Dexamethasone Drug Overdose Fishes Food Infection Insecta Lebistes Males Metacercariae Mice, House Mouse, Swiss Parasites Pentobarbital Sodium Physical Examination Snails Tadpole Water, Body
4
Schistosomiasis Infection and Egg Quantification
Biomphalaria glabrata (M-line) infected with the S. mansoni Puerto Rican strain (PR-1) were obtained from the Schistosomiasis Resource Center of the Biomedical Research Institute (Rockville, MD) through NIH-NIAID Contract HHSN272201700014I for distribution through BEI Resources. Mice were challenged 3 weeks following vaccination with 150 cercariae by tail exposure and were sacrificed 7 weeks post-challenge84 (link). Briefly, adult worms were counted after perfusion of the hepatic portal system and manual removal from the mesenteric veins. The livers and intestines were harvested from each mouse, weighed, and digested in 4% potassium hydroxide overnight at 37 °C. The next day, the number of eggs per gram of tissue was recorded by microscopy.
Hassan A.S., Houle S., Labrie L., Perera D.J., Dozois C.M., Ward B.J, & Ndao M. (2023). Salmonella Typhimurium expressing chromosomally integrated Schistosoma mansoni Cathepsin B protects against schistosomiasis in mice. NPJ Vaccines, 8, 27.
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Publication 2023
Adult Australorbis glabratus Cercaria Eggs Helminths Intestines Liver Microscopy Mus Perfusion Portal System potassium hydroxide Puerto Ricans Schistosomiasis Strains Tail Tissues Vaccination Vein, Mesenteric
5
Rearing Biomphalaria glabrata Snails
Several Biomphalaria glabrata snails were kindly provided by Dr. Yousheng Liang from Jiangsu Provincial Institute of Schistosomiasis Control and Prevention. The Puerto Rico (PR) strain of B. glabrata has been kept and bred in Dr. Liang’s laboratory for 10 years. Snails were continued to produce for two generations in our laboratory before snails collection in this study.
Snails ranging from 10.0 mm to 15.0 mm in shell diameter were randomly selected, placed in a clean container with 1 L aerated dechlorinated tapwater per 15 snails, and reared at 26–28 °C with a 12 h light and 12 h dark photocycle and fed with fresh green-leaf lettuce. The container was cleaned regularly to remove food residue. Small clean cling films were set on the water surface to let snails deposit eggs. The egg masses were collected daily and transferred to a clean container with dechlorinated tap water. The newly hatched snails were kept in 200 ml water in a shallow 500 ml container per 100 snails and fed with dried lettuce and fish food. The juvenile and adult snails were continually transferred to a new container with 1–1.5 L of dechlorinated tap water, fed, and maintained under the same conditions as the above procedure.
Xu S., Zhang Y.W., Habib M.R., Li S.Z., Yuan Y., Ke W.H., Jiang N., Dong H, & Zhao Q.P. (2023). Inhibition of alternative oxidase disrupts the development and oviposition of Biomphalaria glabrata snails. Parasites & Vectors, 16, 73.
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Publication 2023
Adult Australorbis glabratus Fishes Food Lactuca sativa Light Plant Leaves Schistosomiasis Snails Strains

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More about "Australorbis glabratus"

Australorbis glabratus, also known as the giant African snail or the Galba snail, is an important species in the field of parasitology and public health.
This freshwater snail is the primary intermediate host for the trematode parasite Schistosoma mansoni, which causes the debilitating disease schistosomiasis (also known as bilharzia) in humans.
Researchers studying Australorbis glabratus often utilize various cell culture media and supplements to maintain and propagate the snail populations in the laboratory.
RPMI 1640 medium and M199 medium are commonly used for this purpose, providing the necessary nutrients and growth factors.
Antibiotics like penicillin and streptomycin are also frequently added to these media to prevent bacterial contamination.
The addition of fetal bovine serum (FBS) or newborn calf serum (NBCS) to the culture media supplies essential growth factors and hormones to support the snail's development and reproduction.
L-glutamine, a critical amino acid, is another common supplement used to enhance the growth and viability of Australorbis glabratus in the lab.
Maintaining healthy snail populations is crucial for studying the host-parasite interactions between Australorbis glabratus and Schistosoma mansoni.
Researchers often use NMRI mice as the definitive host to propagate the schistosome life cycle and investigate various aspects of schistosomiasis, such as drug efficacy, immune responses, and pathogenesis.
PubCompare.ai's innovative AI-driven platform can be a valuable tool for researchers working with Australorbis glabratus.
This platform helps users effortlessly locate relevant protocols from the literature, preprints, and patents, while leveraging AI-driven comparisons to identify the best protocols and products.
By improving research workflows and enhancing reproducibility, PubCompare.ai can support breakthroughs in the study of this important snail species and its role in schistosomiasis.