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Dermatophagoides farinae

Dermatophagoides farinae is a species of dust mite commonly found in household environments.
These microscopic arachnids are a major source of allergens that can trigger asthma and allergic reactions in susceptible individuals.
Understanding the biology and behavior of D. farinae is crucial for developing effective strategies to mitigate its impact on human health.
PubCompare.ai's AI-powered platform can enhance your research on this important pest by helping you easily locate relevant protocols from literature, preprints, and patents, while providing intelligent comparisons to identify the optimal approaches.
Improve the reproducibility and acuracy of your D. farinae studies with the tools and insights offered by PubCompare.ai.

Most cited protocols related to «Dermatophagoides farinae»

Murine Models of House Dust Mite-Induced Airway Inflammation

Cited 6 times

HDM extract (Greer Laboratories, Lenoir, NC) was re-suspended in normal saline and introduced by intranasal instillation, with the mice lightly anesthetized with 5% isoflurane, using a 20–200 µL pipette tip (Fig. 1). The acute HDM protocol used 100 µg HDM (D. pteronyssinus or D. farinae) in 50 µL saline on days 0–4 and day 11. Saline-exposed control mice received 50 µL sterile saline. The 4-week models used 10 µg or 25 µg D. pteronyssinus in 35 µL of saline. Mice were sensitized for 5 consecutive days weekly (from Monday to Fridays) for 4 weeks. The mice in the 8-week model were sensitized to 25 µg D. pteronyssinus for 5 consecutive days on week 1, followed by every other weekday (Monday, Wednesday and Friday) from weeks 2 to 8. Saline-exposed control mice in the 2-week, 4-week and 8-week models were handled in a similar fashion but were only treated with sterile saline. Evaluation of the endpoint metrics occurred 24 hours after the last HDM or saline exposure.Figure 1

Timelines for allergen exposure in the 3 different HDM models. (A) Acute 2-week model of HDM-induced airways inflammation. Mice were sensitized by intranasal instillation of 100 µg HDM in 50 µl saline or 50 µl saline alone (Control) on days 0–4 and then challenged with 100 µg HDM on day 11. (B) Chronic 4-week model of HDM-induced airways inflammation. Mice were sensitized to 10 µg or 25 µg HDM in 35 µl saline or 35 µl saline alone by intranasal instillation for 5 days/week for 4 weeks. (C) Chronic 8-week chronic model of HDM-induced airways inflammation. Mice were sensitized by intranasal instillation of 25 µg HDM in 35 µl saline or 35 µl saline for 5 days a week in week 1, and every Monday, Wednesday and Friday in weeks 2–8.

Woo L.N., Guo W.Y., Wang X., Young A., Salehi S., Hin A., Zhang Y., Scott J.A, & Chow C.W. (2018). A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling. Scientific Reports, 8, 6925.

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Publication 2018

Allergens Dermatophagoides farinae Dermatophagoides pteronyssinus Inflammation Isoflurane Mus Normal Saline Saline Solution Sterility, Reproductive

Atopic Dermatitis Induction in Mice

Cited 5 times

AD was induced in mice by repeated local exposure of Dermatophagoides farinae extract (DFE; house dust mite extract) and 2,4-dinitrochlorobenzene (DNCB) to the ears, as previously described [17 (link)]. A schematic of the experimental procedure is provided in Figure 1. For the induction of AD, the mice were divided into four groups (control, AD-only, EF-2001-only, and AD + EF-2001), and the surfaces of both earlobes were stripped five times with surgical tape (Nichiban, Tokyo, Japan). After stripping, 20 µL of 1% DNCB was painted onto each ear, followed by 20 µL of DFE (10 mg/mL) four days later. DFE and DNCB treatment was administered once a week for four weeks. Animals received EF-2001 (2 mg/kg orally administered) throughout the four weeks of AD induction.
The ear thickness was measured 24 h after DNCB or DFE application with a dial thickness gauge (Kori Seiki MFG, Co., Tokyo, Japan). At days 14 and 28, blood samples were collected by orbital puncture. Plasma samples were prepared from the cardiac puncture under ketamine/xylazine anesthesia and stored at −70 °C for further analysis. After blood collection, the ears were removed and used for histopathological analysis. The serum immunoglobulin (Ig)E, DFE specific IgE and IgG2a levels were measured at days 14 and 28 after the first induction using an IgE enzyme-linked immunoassay kit (Bethyl Laboratories, Inc., Montgomery, TX, USA) according to the manufacturer’s instructions.

Choi E.J., Iwasa M., Han K.I., Kim W.J., Tang Y., Hwang Y.J., Chae J.R., Han W.C., Shin Y.S, & Kim E.K. (2016). Heat-Killed Enterococcus faecalis EF-2001 Ameliorates Atopic Dermatitis in a Murine Model. Nutrients, 8(3), 146.

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Publication 2016

allobarbital Anesthesia Animals BLOOD Dermatophagoides farinae Dermatophagoides pteronyssinus Dinitrochlorobenzene Ear Enzyme Immunoassay Heart IgG2A Immunoglobulin E Ketamine Mice, House Plasma Punctures Serum Xylazine

Cytokine and Allergen Detection in Tissue Samples

Cited 5 times

Tissue samples were weighed and homogenized and the supernatants were harvested for later analysis [7 (link)]. The protein levels of cytokines and chemokines were detected by means of bio-plex suspension chip technology (BIO-RAD, Hercules, Ca) according to the manufacturer’s instructions. Total IgE and specific IgE levels to common airborne allergens were detected by the ImmunoCAP system (Phadia, Uppsala, Sweden). Specific IgE was determined for house dust mix (Hx2, Dermatophagoides pteronyssinus, Dermatophagoides farinae and Blatella germanica), mould mix (Mx2, Penicillium notatum, Aspergillus fumigatus, Candida albicans and Alternaria), animal epidermal and protein mix (Ex1, Dander from cat, horse, cow and dog), tree pollen mix (Tx4, Oak, Elm, Maple leaf sycamore, Willow and Cottonwood), weed pollen mix (Wx5, Common Ragweed, Mugwort, Marguerite, Dandelion and Golded rod) and staphylococcus aureus enterotoxin A (SEA) and B (SEB) [2 (link), 7 (link), 8 (link)]. More information is provided in the Online Supplement.

Cao P.P., Zhang Y.N., Liao B., Ma J., Wang B.F., Wang H., Zeng M., Liu W.H., Schleimer R.P, & Liu Z. (2014). Increased local IgE production induced by common aeroallergens and phenotypic alteration of mast cells in Chinese eosinophilic, but not non-eosinophilic, chronic rhinosinusitis with nasal polyps. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 44(5), 690-700.

Publication 2014

Acer Allergens Alternaria Animals Artemisia vulgaris Aspergillus fumigatus Candida albicans Chemokine Cytokine Dander Dermatophagoides farinae Dermatophagoides pteronyssinus Dietary Supplements DNA Chips Epidermis Equus caballus Fungus, Filamentous House Dust Penicillium chrysogenum Plant Leaves Pollen Populus fremontii Proteins Staphylococcal enterotoxin A Taraxacum Tissues Trees Willow

Biomarkers of Asthma Exacerbation

Cited 5 times

Adherence to all asthma treatments was assessed by means of study interviews, corroborated by study physicians, and encouraged by asthma counselors every 3 months. Allergen skin testing consisted of a panel of 14 extracts: mouse and rat epithelia, dog epithelium, dust mites (Dermatophagoides farinae and D. pteronyssinus), cat hair, an American–German cockroach mix, German cockroach, molds (Penicillium notatum, aspergillus species, Alternaria tenuis, and Cladosporium herbarum), timothy grass, and a ragweed mix (Greer Laboratories). A positive test was defined as a wheal that was larger than the negative control by 3 mm or more.
Total serum levels of IgE and allergen-specific IgE levels for dust mites, German cockroach, and A. tenuis were measured. Dust from the participant’s bed and bedroom floor was collected with the use of a validated self-collection procedure^{12 (link)} and assayed for dust mite (Der p 1 and Der f 1), German cockroach (Bla g 1), cat (Fel d 1), dog (Can f 1), and mouse (Mus m 1).
Nasal-secretion samples were collected and frozen at four of the eight research sites at week 48 and within 7 days after the onset of an asthma exacerbation. Total RNA was extracted and analyzed by means of multiplex reverse-transcriptase–polymerase-chain-reaction assay^{13 (link),14 (link)} with the use of primers and probes specific for rhinoviruses, influenza virus, parainfluenza virus, coronavirus, respiratory syncytial virus, meta-pneumovirus, enterovirus, adenovirus, and boca-virus.

Busse W.W., Morgan W.J., Gergen P.J., Mitchell H.E., Gern J.E., Liu A.H., Gruchalla R.S., Kattan M., Teach S.J., Pongracic J.A., Chmiel J.F., Steinbach S.F., Calatroni A., Togias A., Thompson K.M., Szefler S.J, & Sorkness C.A. (2011). Randomized Trial of Omalizumab (Anti-IgE) for Asthma in Inner-City Children. The New England journal of medicine, 364(11), 1005-1015.

Publication 2011

Adenovirus Infections allergen Bla g 1 Allergens Alternaria alternata Aspergillus Asthma Brown Oculocutaneous Albinism Cladosporium herbarum Cockroach, German Coronavirus Counselors Dermatophagoides Allergens Dermatophagoides farinae Dermatophagoides farinae antigen f 1 Dermatophagoides pteronyssinus Dermatophagoides pteronyssinus antigen p 1 Enterovirus Epithelium Freezing Fungus, Filamentous Hair Mice, House Nose Oligonucleotide Primers Orthomyxoviridae Parainfluenza Penicillium chrysogenum Periplaneta americana Phleum Physicians Pneumovirus Pyroglyphidae Respiratory Syncytial Virus Reverse Transcriptase Polymerase Chain Reaction Rhinovirus secretion Serum Virus

Genome Sequencing of Dermatophagoides Mites

Cited 4 times

DP were captured in Ohio, USA and maintained in culture for many years. DP growth conditions were previously described [19 ]. DNA isolation from whole mite extract is detailed in the Supplemental Material. Explicit methods regarding genome assembly and protein prediction are also described in the Supplemental Material. All original sequence data used herein are deposited to Genbank under Bioproject PRJNA395246.
All multiple sequence alignments were generated using muscle within CLC Genomics Workbench.
Several genomes from Acari suborder, which includes mites and ticks, have recently been published including D. farinae [5 (link)], scabies mites Sarcoptes scabiei (SS) [20 (link)–22 ], the honey bee mite Tropilaelaps mercedesae [23 ], the spider mite Tetranychus urticae [24 (link)], which is preyed upon by the mite Metaseiulus occidentalis [25 (link)], Ixodes scapularis [26 ,27 (link)], and Varroa destructor [28 (link)]. Four mite genomes from the order Oribatida have also been sequenced: Achipteria coleoptrata, Platynothrus peltifer, Steganacarus magnus, and Hypochthonius rufulus [29 (link)]. Genomic data for these species was downloaded from GenBank (Table 1).

Randall T.A., Mullikin J, & Mueller G.A. (2018). The draft genome assembly of Dermatophagoides pteronyssinus supports identification of novel allergen isoforms in Dermatophagoides species. International archives of allergy and immunology, 175(3), 136-146.

Publication 2018

Acarus Dermatophagoides farinae Genome Growth Disorders isolation Ixodes scapularis Mites Muscle Tissue Proteins Psoroptes ovis Sarcoptes scabiei Sequence Alignment Tetranychidae Ticks Varroa destructor

Most recents protocols related to «Dermatophagoides farinae»

Efficacy of SLIT in Pediatric Dust Mite Allergic Rhinitis

In this study, 153 patients with AR who received SLIT with Dermatophagoides farinae (D. farinae) drops in the Otorhinolaryngology Department of Tianjin Children’s Hospital from January 2020 to October 2021 were selected as the study objects. The patient’s sex, age, serum vitamin D₃ levels, family history of allergic disease, food allergies, their caregiver’s education level, asthma status, whether they had used acarid products, etc. were collected retrospectively. All patients included in this study recorded their visual simulation scores, symptom scores, medication scores, and quality of life scores to evaluate the efficacy of SLIT. The primary endpoint of this study was the efficacy of patients treated with SLIT, and the independent influencing factors affecting the efficacy of SLIT were analyzed.
The enrolled patients were required to fully meet the following conditions: (I) diagnosed with AR, with or without other allergic diseases; (II) 3–14 years old, male or female; (III) allergen detection results: dust mites were positive, closely related to clinical symptoms and were the main allergen; (IV) allergens could be avoided as much as possible during treatment; (V) willing to receive SLIT and able to comply with the program and receive follow-up. The exclusion criteria were as follows: (I) patients with infectious rhinitis; (II) patients who could not understand the purpose of this study and refused to cooperate with follow-up; (III) those with incomplete data. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). This study was approved by the Ethics Committee of Tianjin Children’s Hospital (No. KY2020-47) and informed consent was taken from all the patients’ guardians.

Li L., Cui X., Zhang X., Zheng L., Sun X., Yang C., Shu J, & Liu G. (2023). Serum vitamin D3 deficiency can affect the efficacy of sublingual immunotherapy in children with allergic rhinitis: a retrospective cohort study. Journal of Thoracic Disease, 15(2), 649-657.

Publication 2023

Allergens Asthma Child Cholecalciferol Common Cold Dermatophagoides farinae Ethics Committees, Clinical Food Allergy Hypersensitivity Legal Guardians Males Patients Pharmaceutical Preparations Pyroglyphidae Serum Woman

Mite Antigen Formulations Evaluation

A mite antigen, Dermatophagoides farinae extract (Biostir AD), was purchased from Biostir Inc. (Osaka, Japan). Betamethasone and tetracycline were obtained from Tokyo Chemical Industry (Tokyo, Japan). White petrolatum including 5% (w/w) liquid paraffin was employed as the vehicle and used to prepare 0.1% (w/w) betamethasone ointment and 3% (w/w) tetracycline ointment.

Matsui K., Kobayashi M., Nagano M, & Matsuoka M. (2023). Effects of dual therapy with betamethasone and tetracycline in a NC/Nga mouse model of atopic dermatitis. Journal of Pharmacy & Pharmaceutical Sciences, 26, 11182.

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Publication 2023

Antigens Betamethasone Dermatophagoides farinae Mites Oil, Mineral Ointments Petrolatum Tetracycline

Standardized Aeroallergen Preparation and Panel

Coca’s extracted aeroallergens were prepared and standardized under complete aseptic conditions at the AIU laboratory, as previously described [33 (link),34 ]. Prepared aeroallergens were labeled and stored at 2–8 °C until use. The allergen panel prepared and employed in SPT included ten common aeroallergens: mixed molds (Alternaria, Aspergillus, Penicillium, and Cladosporium species), mixed mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae), date palm pollen, smoke, hay dust, wool, cotton, mixed feather (pigeon, duck, goose, and chicken) cat hair, and dog hair.

Mokhtar G.A., Gebriel M.G., Hammad N.M., Roman S.W., Attia O., Behiry A., Ismail N.A., Sayed M.S., Hadhoud A.N., Osama Y.A., Ali A.A, & Kadry H.M. (2023). Fungal Aeroallergen Sensitization Patterns among Airway-Allergic Patients in Zagazig, Egypt. Journal of Fungi, 9(2), 185.

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Publication 2023

Allergens Alternaria Asepsis Aspergillus Chickens Cladosporium Coca Columbidae Dermatophagoides farinae Dermatophagoides pteronyssinus Ducks Feathers Fungus, Filamentous Geese Gossypium Hair Mites Penicillium Phoenix dactylifera Pollen Smoke

Measuring Total and Specific IgE

Total IgE was measured using an indirect enzyme-linked immunosorbent assay according to the manufacturer’s instructions (Chemux Bioscience, South San Francisco, CA, USA). Total IgE serum levels up to 150 IU/mL were considered normal.
The serum aeroallergen-specific IgE for 13 common aeroallergens (Dermatophagoides farinae, Dermatophagoides pteronyssinus, A. alternata, A. niger, A. fumigatus, P notatum, C. albicans, mixed grasses, birch, cat epithelium, dog epithelium, feather mixture, and cockroach) was analyzed by immunoblot assay according to the manufacturer company protocol (AllergyScreen^® system, MEDIWISS Analytic GmbH, Moers, Germany). Serum-specific IgE was analyzed by a rapid scanner (Improvio Scanner System, Moers, Germany). The test was considered valid if the process control on each strip’s first position was colored. Specific IgE level ≥ 0.35 IU/mL was deemed positive.

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Publication 2023

Betula Candida albicans Cockroaches Dermatophagoides farinae Dermatophagoides pteronyssinus Enzyme-Linked Immunosorbent Assay Epithelium Feathers Immunoblotting Poaceae Serum

Skin Sensitization to House Dust Mites

Sensitization to house dust mite aeroallergens, including Der-p, Dermatophagoides farinae (Der-f), and other common allergens (Soluprick SQ, ALKAbello A/S, Horsholm, Denmark), was assessed. A positive skin reaction was defined as a wheal size ≥3 mm after subtraction of the negative control.

Su Q., Ren N., Feng M., Zeng X., Dong Y., Xian M., Shi X., Luo T., Liu G, & Li J. (2023). Specific immunoglobulin G4 correlates with Th2 cytokine reduction in patients with allergic asthma treated by Dermatophagoides pteronyssinus subcutaneous immunotherapy. The World Allergy Organization Journal, 16(1), 100715.

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Publication 2023

Allergens Dermatophagoides farinae Dermatophagoides pteronyssinus Skin

Top products related to «Dermatophagoides farinae»

Immunocap by Thermo Fisher Scientific

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The ImmunoCAP is a laboratory instrument used for in vitro allergen-specific IgE testing. It provides quantitative measurement of IgE antibodies to a wide range of allergens. The ImmunoCAP system utilizes fluorescent enzyme immunoassay technology to detect and measure IgE levels in patient samples.

Immunocap system by Thermo Fisher Scientific

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Dermatophagoides farinae by Stallergenes Greer

Sourced in United States

Dermatophagoides farinae is a species of dust mite that is commonly used as a laboratory specimen. It is a small, microscopic arthropod that is a member of the Acaridae family. The Dermatophagoides farinae mite is often utilized in research and testing related to allergens and immune responses.

Immunocap phadiatop infant by Thermo Fisher Scientific

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The ImmunoCAP Phadiatop Infant is a laboratory diagnostic tool designed to detect the presence of specific IgE antibodies in infant blood samples. It is used to screen for a range of common environmental and food allergens.

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Immunocap 100 by Thermo Fisher Scientific

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The ImmunoCAP assay is a laboratory test designed to detect and measure specific antibodies in a person's blood. It is used to identify the presence and levels of immunoglobulin E (IgE) antibodies, which are associated with allergic responses. The ImmunoCAP assay provides quantitative results, allowing for the assessment of the patient's allergic sensitivity to various environmental and food-related allergens.

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More about "Dermatophagoides farinae"

Dermatophagoides farinae, commonly known as the American house dust mite, is a species of microscopic arachnid that is a major source of household allergens.
These tiny creatures, also referred to as D. farinae or dust mites, can trigger asthma and allergic reactions in individuals who are susceptible.
Understanding the biology and behavior of Dermatophagoides farinae is crucial for developing effective strategies to mitigate its impact on human health.
The ImmunoCAP system, including ImmunoCAP Phadiatop Infant, is a valuable tool for detecting and measuring specific IgE antibodies to D. farinae allergens.
Researchers can utilize PubCompare.ai's AI-powered platform to enhance their studies on this important pest.
The platform helps locate relevant protocols from literature, preprints, and patents, while providing intelligent comparisons to identify the optimal approaches.
This can improve the reproducibility and accuracy of Dermatophagoides farinae studies, using tools and insights like Falcon tubes, ImmunoCAP 100, and the ImmunoCAP assay.
Additionally, exploring the role of OVA (ovalbumin) and Anti-IL-13-PE (anti-interleukin-13 phycoerythrin) can provide valuable insights into the immune responses and potential therapeutic interventions related to Dermatophagoides farinae exposure.
The Vacuette system may also be useful for collecting and processing samples in these studies.
By leveraging the available resources and technologies, researchers can enhance their understanding of Dermatophagoides farinae and develop more effective strategies to mitigate its impact on human health.