The largest database of trusted experimental protocols
> Living Beings > Eukaryote > Mosquito Vectors

Mosquito Vectors

Mosquito Vectors: Mosquitoes that can transmit diseases to humans or animals.
This term encompasses a wide range of mosquito species capable of serving as vectors for pathogens such as malaria, dengue, Zika, and others.
Understanding the biology, behavior, and ecology of these mosqutio vectors is crucial for developing effective prevention and control strategies against vector-borne diseases.
Researchers can optimize their mosquito vectors research using PubCompare.ai, an AI-powered platform that helps locate the best protocols from literature, preprints, and patents, enabling identification of the most accurate and reproducible methods to enhance the quality and effiency of their work.

Most cited protocols related to «Mosquito Vectors»

The Mimika district lies on the southern coast of Papua in Eastern Indonesia (Figure 1), covering an area of 21,522 square-kilometres with 12 sub-districts and 85 villages. The area is largely forested with both coastal and mountainous areas. Each year a total of approximately 5.5 metres of rainfall is recorded with peaks in July to September and December (unpublished data). At the last census in 2004, the population in the lowlands was reported as 130,000. One hospital, the Rumah Sakit Mitra Masyarakat (RSMM) in the town of Timika, services the whole district and is the only hospital available for the lowland population. Due to the presence of a local mine, there is economic migration, with the local population increasing by an estimated 16% per year. This has resulted in the diverse ethnic origin of the local population, with highland Papuans, lowland Papuans and non-Papuans all resident in the region. Healthcare for the population is provided by the public clinics of the local ministry of health, the Public Health Malaria Control programme (PHMC) of the mine, the RSMM hospital and the private sector.
Malaria transmission is perennial, but restricted to the lowland area where it is associated with three mosquito vectors: Anopheles koliensis, Anopheles farauti and Anopheles punctulatus [11 (link),12 (link)]. Entomological inoculation rates vary between 1 and 4 infected bites per year (unpublished data). Bed net coverage is estimated to be approximately 40%. In view of the high number of infections in non-immune patients, local protocols recommend that all patients with patent parasitaemia at any level are given antimalarial therapy. At the time of the study local treatment guidelines advocated chloroquine plus sulphadoxine-pyrimethamine for P. falciparum and chloroquine monotherapy for non-falciparum malaria. An assessment of local treatment regimens in 2004 highlighted that the day-28 cure rate of chloroquine monotherapy was less than 35% for patients with P. vivax and that of chloroquine plus sulphadoxine-pyrimethamine was only 52% for patients with P. falciparum [7 (link)]. Local and national guidelines also recommend that patients with P. vivax parasitaemia over 1 years old, receive 14 days unsupervised treatment with primaquine, however adherence to and effectiveness of this regimen in this setting is not known.
Full text: Click here
Publication 2008
Anopheles Antimalarials Bites Chloroquine Infection Malaria Malaria, Falciparum Mosquito Vectors Parasitemia Patients Primaquine Private Sector sulphadoxine-pyrimethamine Transmission, Communicable Disease Treatment Protocols Vaccination

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2007
Adult Anopheles Antimalarials artemisinine artenimol Child Chloroquine chrysarobin Coartem Coinfection Combined Modality Therapy Ethics Committees Ethics Committees, Research Hypersensitivity Infection Lumefantrine, Artemether Malaria Microscopy Mosquito Vectors Parasitemia Parasites Parent Patients Pharmaceutical Preparations piperaquine Recrudescence Transmission, Communicable Disease Visually Impaired Persons
The study was carried out at an established rural outpatient clinic west of the city of Timika in southern Papua, Indonesia. This forested lowland area has unstable malaria transmission associated with three mosquito vectors: Anopheles koliensis, A. farauti and A. punctulatus (Lee et al., 1980 (link), Pribadi et al., 1998 (link)). The annual incidence of malaria is 620 per 1000 per year, divided 55:45 between P. falciparum and P. vivax infections (Peter Ebsworth, personal communication). Owing to economic migration, the ethnic origin of the local population is diverse, with highland Papuans, lowland Papuans and non-Papuans all resident in the region. In view of the high number of infections in non-immune patients, local protocols recommend that all patients with patent parasitaemia are given antimalarial therapy.
Full text: Click here
Publication 2007
Anopheles Antimalarials Infection Malaria Mosquito Vectors Parasitemia Patients Therapeutics Transmission, Communicable Disease
The study was carried out at Rumah Sakit Mitra Masyarakat (RSMM) hospital, Timika, in the southern lowlands of Papua, Indonesia. The hospital has 110 beds with a high-dependency unit and an emergency department open 24 h a day. The outpatient department reviews approximately 300 patients per day, 6 d per week. RSMM is the only hospital in the district, servicing a population of approximately 150,000 people spread over an area of 21,522 km2. The area is largely forested with both coastal and mountainous areas. Malaria transmission is restricted to the lowland area where it is associated with three mosquito vectors: Anopheles koliensis, An. Farauti, and An. punctulatus [11 (link),12 (link)]. The annual incidence of malaria in the region is 885 per 1,000 person years, divided 62:38 between P. falciparum and P. vivax infections (unpublished data). In 2005 a household survey rate found 7.4% of respondents to be positive for P. falciparum, 6.4% for P. vivax, and 1.9% mixed infection with both species (unpublished data). Due to economic migration the ethnic origin of the local population is diverse, with highland Papuans, lowland Papuans, and non-Papuan migrants all resident in the region. Hospital policy dictates that all patients presenting with history of fever and all pregnant women irrespective of symptoms should have a blood film examination for malaria.
Publication 2008
Anopheles Blood Coinfection Fever Households Infection Malaria Migrants Mosquito Vectors Outpatients Patients PER1 protein, human Pregnant Women Transmission, Communicable Disease
In Kigoche village, five houses spaced apart at a distance of at least 25 m and at least 100 m away from rice paddies were selected for the study. A 5 × 5 Latin square experimental design preceded by a 5 d trial period was run to assess the potential of MB in attracting malaria and other mosquito vectors. The treatments, assigned to each of the five houses on rotational basis per night, included a human host, IB1, SB, MB, and an empty house i.e., the control. All components of the synthetic attractants, with the exception of carbon dioxide, were delivered via nylon strips (Okumu et al., 2010a (link)) and dispensed using MM-X traps. The traps were suspended 15 cm above a bed inside unimpregnated mosquito nets, and were operated using 12 volt batteries. One of the fans on the MM-X trap was disabled to prevent it from trapping mosquitoes. Mosquitoes were trapped by hanging an unlit CDC miniature light trap (Model 512; John W. Hock Company, Gainesville Fl., USA) operated on 6 V batteries (Gaston Battery Industry Ltd, China) beside and at 15 cm (Jawara et al., 2009 ) above the odor outlet tube of the MM-X trap, outside the bed net. Both MM-X and CDC light traps were hung on the foot end region of the beds in all cases (Mboera et al., 2000 (link)). Only one MM-X trap and one CDC light trap were used in houses where mosquito catches were based on synthetic attractants. Only one CDC light trap was used in houses occupied by human subjects. The volunteer slept inside a well tucked-in bed net with a CDC light trap suspended besides it. Whereas a total of five adult men aged 18–25 years volunteered to participate in the study, only one individual participated per night. The volunteers rotated over the treatments according to a random design. Each experimental night lasted from 1900 to 0630 h. After the experiments traps were disconnected from the batteries, the trapped mosquitoes were taken to the laboratory, where they were killed by freezing at −4°C. Traps were cleaned with 30% methanol solution before being reused. During experimental nights, the residents of the study houses were not present. Other houses in the village were occupied normally.
Full text: Click here
Publication 2012
Adult Ankle Carbon dioxide Culicidae Foot Homo sapiens Light Malaria Methanol Mosquito Nets Mosquito Vectors Nylons Odors Oryza sativa Sleep Voluntary Workers

Most recents protocols related to «Mosquito Vectors»

Descriptive and inferential statistical analyses were used in the present study. Various life table parameters such as fecundity, hatchability, pupation percentage, eclosion percentage and male:female ratio were recorded and are presented in graphical forms. For eggs and wing measurements, statistical t-test analysis for independent or correlated samples was performed using Vassar Stat software (http://vassarstats.net/). The mean and SEM were compared between populations. A p-value > 0.05 was considered non-significant for each parameter. Insecticide susceptibility was evaluated using the Prism GraphPad software package. The vectorial competence between the mosquito populations was analyzed using a non-parametric Mann-Whitney test using Prism GraphPad.

Schematic representation of the establishment of isofemale lines

Full text: Click here
Publication 2023
Eggs Females Fertility Insecticides Males Mosquito Vectors Population Group prisma Susceptibility, Disease
The vector infestation of the A. aegypti mosquito was estimated from the Building Infestation Index (BII), which assesses the risk situation of arbovirus transmission by measuring the population level of the vector when calculating the percentage of properties with the presence of larvae of this Culicidae within the total number of surveyed properties24
. The calculation is performed using the Rapid Index Survey for A. aegypti (Levantamento Rápido de Índices para A. aegypti – LIRAa) and the BII is classified as: satisfactory (<1%), alert (1–3.9%), and at risk (>3.9%)24
. These data were provided by the Environmental Surveillance of 1st GERES/SES-PE.
Full text: Click here
Publication 2023
Arboviruses Cloning Vectors Culicidae Larva Mosquito Vectors Parasitic Diseases Transmission, Communicable Disease
The study was conducted during the peak malaria season (September to December) in 2017 in Thiès (Senegal; 14° 47′ 26″ north, 16° 55′ 29″ west), an area belonging to the Sahelian facies defined by a short malaria seasonal transmission (< 4 months). In this region, the entomological inoculation rate (EIR) is low, estimated to be < 5 [29 (link)], and malaria is mainly transmitted by Anopheles arabiensis and Anopheles gambiae mosquito vectors.
Full text: Click here
Publication Preprint 2023
Anopheles Anopheles gambiae Face Malaria Mosquito Vectors Vaccination

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Adult Aedes Animals Biopharmaceuticals Chinese Climate Culex Culicidae Dengue Fever Dry Ice Electricity Females Forests Genes Homo sapiens Infection Mosquito-Borne Diseases Mosquito Vectors Oxidase, Cytochrome-c Protein Subunits PTGS1 protein, human Satiation Ultraviolet Rays
We revisit a recently published compendium of occurrence data for 22 species of Anopheles mosquitoes vectors of malaria in Africa [23 (link)]. While some of these data are resolved to finer taxonomy, we used the broadest possible definitions at the species level, treating Anopheles funestus sensu lato and sensu stricto as one species, and all members of the Anopheles gambiae complex—including An. gambiae s.l., s.s., M form, and S form—as another single species. (We elect not to stratify these further, given that some are a nested subset of others, in ways that would be challenging to develop ad hoc rules around.)
In total, the dataset comprises over a century's (1898–2016) worth of long-term, systematic entomological surveys from malaria programmes, as well as other opportunistic data collected by researchers, gathered from a mix of peer-reviewed publications, technical reports, theses, and archival records. Records span more than 1 year at the majority of sampling sites (61%), covering an average of 8.5 years between the first and last presence record (figure 1b). Parsed into unique spatio-temporal records, the dataset includes a total of 5 04 314 year-locality pairs, with an average of 22 923 records per species. While sampling fluctuates over time and increases during the Global Malaria Eradication Program (GMEP; 1955–1969), the dataset spans the entire century with an incredible level of detail (electronic supplementary material, figure S1).

(a) The elevational gradient in Africa (averaged to a 10 × 10 fold higher resolution for visual clarity; (b) sites of Anopheles mosquito occurrence in sub-Saharan Africa, where colour represents the maximum temporal span of observations.

For elevational data, we used the GTOPO30 global digital elevation model downloaded as a 30 arc-second resolution grid for Africa from Data Basin (www.databasin.org; figure 1a). We extracted elevation for each distinct occurrence record, using the ‘raster' package in R version 3.3.2. In each year, we extracted the highest-elevation and southernmost records by species. A total of 116 unique locality points, situated on coastlines or islands (e.g. Cape Verde), were beyond the spatial extent of the raster and so were not assigned elevational data.
Full text: Click here
Publication 2023
Anopheles Anopheles gambiae Culicidae Malaria Mosquito Vectors

Top products related to «Mosquito Vectors»

Sourced in United States
The Misonix Sonicator 3000 is a laboratory equipment used for sonication, a process that utilizes high-frequency sound waves to disrupt the cell membranes of biological samples. The device generates and delivers ultrasonic energy to facilitate the extraction, homogenization, or cell lysis of various materials.
Sourced in Germany, United States, United Kingdom, Japan, China, Spain, Netherlands, Italy
The DNA extraction kit is a laboratory equipment designed to isolate and purify DNA from various biological samples. It provides a standardized and efficient method for extracting DNA, which is a fundamental step in many molecular biology applications.
Sourced in United States
Stata/MP version 15.1 is a statistical software package developed by StataCorp. It is designed to perform advanced data analysis, statistical modeling, and visualization tasks. Stata/MP provides enhanced computational capabilities to handle large datasets and complex analyses.
Sourced in United States, United Kingdom, Germany, China, Japan, Canada, France, Switzerland, Italy, Australia, Belgium, Spain, Denmark, Ireland, Netherlands, Holy See (Vatican City State), Israel
Opti-MEM is a cell culture medium designed to support the growth and maintenance of a variety of cell lines. It is a serum-reduced formulation that helps to reduce the amount of serum required for cell culture, while still providing the necessary nutrients and growth factors for cell proliferation.
Sourced in Japan
The PCR Thermal Cycler Dice TP600 is a laboratory instrument used for the amplification of DNA sequences through the Polymerase Chain Reaction (PCR) process. It provides precise temperature control and cycling capabilities required for efficient DNA amplification.
Sourced in United States, China
CCL-81 is a cell line derived from the kidney of an African green monkey (Cercopithecus aethiops). It is a commonly used cell line in research applications.
Sourced in United States, Austria, Japan, Cameroon, Germany, United Kingdom, Canada, Belgium, Israel, Denmark, Australia, New Caledonia, France, Argentina, Sweden, Ireland, India
SAS version 9.4 is a statistical software package. It provides tools for data management, analysis, and reporting. The software is designed to help users extract insights from data and make informed decisions.
Sourced in United States, Japan, United Kingdom, Germany, Austria, Belgium, Denmark, China, Israel, Australia
SPSS version 21 is a statistical software package developed by IBM. It is designed for data analysis and statistical modeling. The software provides tools for data management, data analysis, and the generation of reports and visualizations.
Sourced in China
Defibrinated sheep blood is a type of laboratory reagent used in various microbiological and clinical applications. It is prepared by collecting sheep blood and removing the fibrin through a process called defibrination, resulting in a liquid blood product that is free of clots. This product can be used for the cultivation and identification of microorganisms, as well as in diagnostic tests and procedures.

More about "Mosquito Vectors"

Mosquito Vectors: Mosquitoes that can transmit diseases to humans or animals, encompassing a wide range of species capable of serving as vectors for pathogens like malaria, dengue, Zika, and others.
Understanding the biology, behavior, and ecology of these mosquito vectors is crucial for developing effective prevention and control strategies against vector-borne diseases.
Researchers can optimize their mosquito vectors research using PubCompare.ai, an AI-powered platform that helps locate the best protocols from literature, preprints, and patents, enabling identification of the most accurate and reproducible methods to enhance the quality and effciency of their work.
Mosquito-borne illnesses, such as malaria, dengue fever, Zika virus, and chikungunya, are a major global health concern.
Mosquito vectors, the mosquito species that can transmit these diseases, are a key focus for researchers and public health professionals.
Understanding the biology, behavior, and ecology of these mosquito species is crucial for developing effective prevention and control strategies.
PubCompare.ai is an innovative AI-powered platform that can help researchers optimize their mosquito vectors research.
By providing advanced comparisons of protocols from literature, preprints, and patents, PubCompare.ai enables researchers to identify the most accurate and reproducible methods, enhancing the quality and efficiency of their work.
This can be particularly useful when utilizing tools like the Misonix sonicator 3000, DNA extraction kits, Stata/MP version 15.1, Opti-MEM, PCR Thermal Cycler Dice TP600, CCL-81 cell line, SAS version 9.4, SPSS version 21, and defibrinated sheep blood.
By leveraging the power of AI-driven protocol optimization, researchers can streamline their mosquito vectors research, leading to more reliable and impactful findings that contribute to the fight against vector-borne diseases.
The platform's ability to identify the best protocols from a vast array of sources helps researchers save time and resources, while ensuring the accuracy and reproducibility of their work.