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Penaeus

Penaeus is a genus of shrimp within the family Penaeidae, commonly known as penaeid shrimp or prawns.
These crustaceans are of significant economic importance, as they are widely farmed and harvested for food consumption globally.
Penaeus shrimp are found in warm coastal waters, inhabiting estuaries, mangrove swamps, and offshore environments.
They undergo a complex life cycle, migrating between inshore and offshore areas.
Penaeus shrimp are characterized by their elongated bodies, segmented exoskeletons, and a pair of large, fan-like appendages used for swimming.
This genus comprises several commercially important species, such as the Pacific white shrimp (Penaeus vannamei) and the tiger prawn (Penaeus monodon), which are extensively cultured in aquaculture systems worldwide.
Researchers studying Penaeus shrimp can utilize the PubCompare.ai platform to access the most accurate and reproducible protocols from literature, pre-prints, and patents, as well as identify the best products and procedures to streamline their Penaeus studies.

Most cited protocols related to «Penaeus»

In the study, 62 strains from the collection of the Department of Industrial and Food Microbiology at the University of Warmia and Mazury in Olsztyn were used. Strains were isolated from 117 samples of raw fish meat and 39 raw shrimps, including 58 samples of Atlantic salmon (Salmo salar), 59 samples of rainbow trout (Oncorhynchus mykiss) and 39 samples of prawns (Penaeus monodon). Strains were grown from frozen stocks, kept in the Microbank (Biomaxima, Lublin, Poland) at −80 °C in 5 mL of Brain Heart Infusion broth (Merck, Darmstadt, Germany) overnight at 37 °C.
Isolation of Enterobacterales was performed by putting together 10 g of raw fish with 90 mL of saline, then a series of 10-fold dilutions were performed in agreement with standard methods for initial suspension and decimal dilutions of test samples for microbiological examination (ISO 6887-1:2017-05) [31 ]. A total of 0.1 mL of the dilution was inoculated onto violet red bile glucose (VRBG) agar and incubated for 24 h at 37 °C. In order to confirm that typical colonies belong to the order Enterobacterales, an oxidase test was performed. Additionally, 10 strains from the American Type Culture Collection (ATCC) were identified as reference strains.
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Publication 2022
Agar Bile Brain Fishes Food Microbiology Freezing Glucose Heart isolation Meat Oncorhynchus mykiss Oxidases Penaeus Saline Solution Salmo salar Strains Technique, Dilution Viola
We use six multi-gene datasets and a single-gene HIV dataset, and each dataset includes one or more outgroup species defined by the original papers (Table 1).

Summary of the biological datasets

DatasetSpeciesGenesOutgroupsDownload
Plants [23 ]104852Monomastix opisthostigma,DOI 10.1186/2047-217X-3-17
Uronema sp.,
Nephroselmis pyriformis,
Pyramimonas parkeae
Mammals [36 ]37424ChickenDOI 10.13012/C5BG2KWG
Insects [31 (link)]1441478IXODES SCAPULARIS,http://esayyari.github.io/InsectsData
Symphylella vulgaris,
Glomeris pustulata,
Lepeophtheirus salmonis,
DAPHNIA PULEX,
Cypridininae sp,
Sarsinebalia urgorii,
Celuca puligator,
Litopenaeus vannamei
Cannon [32 (link)]78213Salpingoeca rosetta,DOI 10.5061/dryad.493b7
Monosiga brevicollis
Mnemiopsis leidyi,
Pleurobrachia bachei,
Euplokamis dunlapae
Rouse [33 (link)]26393Mnemiopsis leidyi,DOI 10.5061/dryad.79dq1
Amphimedon queenslandica,
Trichoplax adhaerens,
Nematostella vectensis
Frogs [39 ]16495Latimeria chalumnae,DOI 10.5061/dryad.12546.2
Protopterus annectens,
Homo sapiens,
Crocodylus siamensis,
Gallus gallus,
Ichthyophis bannanicus,
Batrachuperus yenyuanensis,
Andrias davidianus
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Publication 2018
Biopharmaceuticals Chickens Daphnia Genes Homo sapiens Lepeophtheirus salmonis Multiple Birth Offspring Penaeus Trichoplax

Samples of shrimp Litopenaeus vannamei (No. 20141215) were collected from a farmed pond with high mortality in a farm in Taizhou of Zhejiang Province in December 2014 for viral isolation and metagenomic analysis. For nested PCR tests, L. vannamei, Fenneropenaeus chinensis, Macrobrachium rosenbergii, and Marsupenaeus japonicas were collected from shrimp farms in the coastal provinces of China during 2014 and 2015. Additionally, healthy juveniles of L. vannamei (mean body weight, 3 g) for challenge test were purchased from a shrimp farm in Weifang of Shandong Province, China. The shrimp samples were demonstrated to be free of WSSV, IHHNV, VPAHPND, YHV and TSV by PCR or RT-PCR methods recommended by the World Organization for Animal Health11 ,12 .
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Publication 2017
Animals Body Weight Infectious hypodermal and hematopoietic necrosis virus isolation Macrobrachium Metagenome Nested Polymerase Chain Reaction Penaeus Reverse Transcriptase Polymerase Chain Reaction
Evolutionary analyses and comparative genomics were performed with 16 species: D. melanogaster, A. gambiae, D. pulex, L. salmonis, S.maritima, S. mimosarum, M. martensii, I. scapularis, H. dujardini, C. elegans, B. malayi, T. spiralis, M. musculus, H. sapiens, and B. floridae. For orthologous group analyses, gene families were identified using OrthoFinder (Emms and Kelly, 2015 (link)). The canonical proteome was used as a query in BlastP against proteomes from 16 species to generate a distance matrix for OrthoFinder to normalize and then cluster with MCL. Detailed methods are described in Source code 5. For the comparative BLAST analysis, five additional transcriptome datasets were used from the following crustacean species: Litopenaeus vannamei, Echinogammarus veneris, Eucyclops serrulatus, Calanus finmarchicus, Speleonectes tulumensis.
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Publication 2016
Biological Evolution Caenorhabditis elegans Crustacea Drosophila melanogaster Genes Muscle Tissue Penaeus Proteome Transcriptome
Functional annotation of the transcriptomes of F. candida and O. cincta was performed using the Blast2GO suite [46 (link)]. BlastX [43 (link)] searches of nucleotide query sequences translated in all reading frames were performed against a non-redundant (nr) protein sequence database [47 (link)] with an e-value cut-off of 10–3. Top hits with the best alignment and the lowest e-value were selected for each contig. Gene Ontology (GO) terms associated to the hits obtained after a BLAST search were retrieved. GO annotation was executed using the following settings: terms with e-value < 10–6, annotation cut-off 50 and GO-Weight of 5. Functional annotation information was obtained from InterPro databases [48 (link)] using the InterProScan [49 (link)] option in Blast2GO.
Since the largest number of top Blast hits was achieved with Daphnia pulex (D. pulex) both for F. candida and O. cincta, the complete genome of D. pulex was selected for further comparative analysis. According to several published phylogenies Daphnia is one of the most closely related outgroups to springtails and insects [3 (link), 10 (link), 50 (link)]. Furthermore, we used protein-coding gene sequences and corresponding amino acid sequences of two other crustaceans Litopenaeus vannamei (L. vannamei) and Penaeus monodon (P. monodon) from EvidentialGene [51 ], and three insects, Pediculus humanus (P. humanus), Tribolium castaneum (T. castaneum) and Acyrthosiphon pisum (A. pisum) from Ensembl Metazoa database [52 (link)] as input for comparative analysis.
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Publication 2015
Amino Acid Sequence Base Sequence Candida Crustacea Daphnia Gene Annotation Genes Genome Insecta Lice, Body Metazoa Open Reading Frames Penaeus Pisum Reading Frames Transcriptome Tribolium, monocots

Most recents protocols related to «Penaeus»

Possible carrier samples (wild shrimps and wild crabs) were collected inside and outside the farms using the convenience sampling method. In each sampling, five carriers from each species were pooled as one sample. In this study, the sites of carrier collection were divided into two categories: (1) Inside the farms and surrounding areas, and (2) outside the farms. Inside the farms and the surrounding areas, possible carrier samples were collected at night in reservoirs, inlet canals, drainage ponds, and communal canals (15 stations × 3 seasons × 60 carriers = 2,700/5 = 540 pooled samples). The specimens sampled were banana shrimp (Fenneropenaeus merguiensis, n = 21), jinga shrimp (Metapenaeus affinis, n = 16), banded snapping shrimp (Alpheus euphrosyne, n = 2), dwarf prawn (Macrobrachium equidens, n = 53), whiteleg shrimp (Litopenaeus vannamei, n = 12), green tidal crabs (Varuna yui, n = 208), and mangrove crabs (Episesarma spp., n = 228). Outside the farms, possible carriers were collected from water sources along the coastline of Rayong Province (11 stations × 3 seasons × 60 carriers = 1980/5 = 396 pooled samples). The samples consisted of banana shrimp (n = 9), Jinga shrimp (n = 8), dwarf prawn (n = 25), whiteleg shrimp (n = 1), green tidal crabs (n = 187), and mangrove crabs (n = 166). In total, 936 pooled samples were sent to a DOF laboratory for WSD detection using nested PCR.
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Publication 2023
affinis Banana Carcinus maenas Drainage Dwarfism Louses, Crab Macrobrachium Metapenaeus Nested Polymerase Chain Reaction Penaeus Pulp Canals
Bacteria were isolated from gilthead sea bream (Sparus aurata L.) and shellfish (Penaeus indicus H. Milne-Edwards). These samples were obtained from a local market in Hail region-Saudi on 25 February 2022. Fish with red spots on their skin were targeted, as this is an indication of microbial infection. Upon arrival, gilthead sea bream and prawns were immediately washed using sterile seawater, gutted, headed, and shucked with a sterile knife. Twenty-five grams from prawn abdomen meat, and the intestines, gills, and muscle meat from S. aurata were enriched in 225 mL of alkaline peptone water supplemented with 1% NaCl [18 (link)]. The inoculated broth media was incubated overnight at 37 °C. After incubation, a loopful from each enrichment culture was steaked onto thiosulfate–citrate–bile salt–sucrose agar (TCBS) (Agar; Sigma Aldrich, Darmstadt, Germany) and onto Vibrio ChromoSelect agar (Sigma Aldrich, Germany), before incubating for 18 to 24 h at 37 °C.
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Publication 2023
Abdominal Cavity Agar Bacteria Citrates Exanthema Fishes Gills Infection Intestines Meat Muscle Tissue Penaeus Peptones Salts, Bile Shellfish Skin Sodium Chloride Sparus Sparus aurata Sterility, Reproductive Sucrose Thiosulfates Vibrio
Red shrimp (Aristeus antennatus) and camarote prawn (Melicertus kerathurus) fresh samples were purchased from a local market (Valencia, Spain), during May 2021 and then transported refrigerated to the University of Valencia. Shrimps were processed by removing the head and body shell (carapace). The side streams (head and shells) were weighted and frozen at −40 °C for 48 h. Afterward, they were freeze-dried (2.5 FREEZONE, LABCONCO, Kansas City, MO, USA) for 72 h. Freeze-dried samples were ground and frozen at −25 °C until the extraction process. A portion of fresh shrimp side streams was refrigerated for some hours at 4 °C until PEF treatment, being frozen and freeze-dried after PEF treatment as described above for the other samples.
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Publication 2023
Animal Shells Freezing Head Human Body Penaeus
WSSV-infected gill tissues (1 g) from penaeid shrimp (Penaeus vannamei) were homogenized in 20 ml of TN buffer [20 mM tris-HCl and 400 mM NaCl (pH 7.5)]. After centrifugation at 3000g for 5 min, the supernatant was collected and further filtered by 0.45-μm filter. A 0.1-ml sample of supernatant was injected intramuscularly into healthy crayfish (Procambarus clarkii, 7 to 10 cm in length and 20 to 30 g in weight) between the third and fourth abdominal segments. After 4 to 7 days, dead and moribund crayfish were collected and kept at 4°C for virus isolation.
The tissues of 10 infected crayfish except for hepatopancreas were homogenized on ice for 5 min using a mechanical homogenizer (SCIENTZ, China) in 1 liter of TNE buffer [50 mM tris-HCl, 500 mM NaCl, and 5 mM EDTA (pH 8.0)] containing protease inhibitors (1 mM phenylmethylsulfonyl fluoride, 1 mM benzamidine, and 1 mM Na2S2O5) (34 (link)). After centrifugation at 3500g for 5 min at 4°C, the supernatant was collected and then subjected to ultracentrifugation at 30,000g for 30 min at 4°C (Beckman Optima XPN-100, USA). The supernatant was discarded, and the upper loose layer of the pellet was rinsed out carefully. The lower solid layer containing viral particles was resuspended in 50 ml of TNE buffer. The resuspension was subjected to another round of successive centrifugation steps at 3500g for 5 min and at 30,000g for 20 min at 4°C. The lower solid layer was resuspended in 1 ml of TM buffer [50 mM tris-HCl and 5 mM MgCl2 (pH 7.5)]. The final resuspension was centrifuged three to five times at 650g for 5 min to remove pink impurities. The milk-like fraction containing intact WSSV virions was stored at 4°C, and fresh samples were subjected to the following treatments.
Purified intact WSSV virions were mixed with an equal volume of 0.2% Triton X-100 in TM buffer and incubated at room temperature for 30 min to obtain oval-shaped WSSV capsids. The sediments were collected after centrifugation at 20,000g for 20 min at 4°C and resuspended in TM buffer. The treatment was repeated once to ensure that envelopes were removed completely. To obtain rod-shaped WSSV capsids, the Triton X-100–treated WSSV capsids were further mixed with an equal volume of 2 M NaCl in TM buffer and incubated at 4°C for 30 min. The sediments were collected after centrifugation at 20,000g for 20 min at 4°C and resuspended in TM buffer. Both rod-shaped and oval-shaped WSSV capsids were immediately subjected to high-resolution cryo-EM analysis.
In addition, the purified intact WSSV virions were directly treated with 2 M NaCl in TM buffer at 4°C for 30 min. The sediments were collected and suspended in TM buffer after centrifugation at 20,000g for 20 min at 4°C. The virions were still envelope-encapsulated, and we named them the capsid-detached WSSV virions. The capsid-detached WSSV virions were collected for the further infection assays.
Publication 2023
Abdomen Astacoidea benzamidine Biological Assay Buffers Capsid Capsid Proteins Centrifugation Edetic Acid Gills Hepatopancreas Infection isolation Magnesium Chloride Milk Penaeidae Penaeus Phenylmethylsulfonyl Fluoride Procambarus Protease Inhibitors Sodium Chloride Tissues Triton X-100 Tromethamine Ultracentrifugation Virion Virus
In practice, every year, the RSF system in LSAs and HSAs in the coastal MD consists of two phases (desalinization and salinization), with six sampling stages (stages 1–6) and two cultivation cycles (rice: July–December; shrimp: January–June) (Fig. 3).

Schematic of stages of RSF practice in LSAs and HSAs, Bac Lieu province.

Fig. 3
In Bac Lieu, the average cultivation area of the model is 1.0–2.5 ha. A well-known local rice (Oryza sativa) variety, such as “one red bush,” and other modern varieties (OM5451, OM2017, HS182) that are salt tolerant are recommended to be grown in this area. The land is prepared after 2–3 rainfalls at the beginning of rainy season. Farmers are recommended to apply CaCO3 or dolomite in combination with soil tilling. The lime mostly used in this research area was a commercial product made from limestone [11 ], with CaO and MgO contents of about 54%–57% and 17%–21%, respectively. The amount of liming material needed is 1.0–1.5 Mg ha−1 [12 ]. During land preparation, the soil is submerged with rainwater or pumped canal water (CW; 2–3 days) to a depth of 10 cm above the field surface. Salt is leached by pumping water from the field to the canal, and the salt leaching cycle is performed three times. Farmers start sowing seed from August to September for long-growth period rice varieties, such as “a red bush” (duration 120 days), which is a local rice variety; or from September to October for short-growth period rice varieties.
Approximately 7–10 days before rice harvesting, water is drained from the field to facilitate harvesting. After rice harvesting, farmers dredge the mud in the ditch bottom. An amount of 0.9–1.3 mg ha−1 of CaCO3 is cast over its bottom and the pond is dried for 15–30 days. After that, the pond is filled with brackish tidewater, submerged in the soil for 5–7 days, and drained. This procedure is repeated about three times. The purpose of draining before the stocking of shrimp (Penaeus monodon) post-larvae is to remove the environmental contaminants that could be harmful to organisms in the soil.
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Publication 2023
Carbonate, Calcium CD3EAP protein, human Desiccation dolomite Farmers Hydrocephalus due to congenital stenosis of aqueduct of sylvius Larva Lichen Sclerosus et Atrophicus Limestone Oryza sativa Penaeus Pulp Canals Rain Sodium Chloride

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More about "Penaeus"

Penaeus, a genus of shrimp within the Penaeidae family, are a group of crustaceans of significant economic importance.
These penaeid shrimp, also known as prawns, are widely farmed and harvested for food consumption around the world.
Penaeus shrimp inhabit warm coastal waters, including estuaries, mangrove swamps, and offshore environments, and undergo a complex life cycle, migrating between inshore and offshore areas.
The Penaeus genus comprises several commercially crucial species, such as the Pacific white shrimp (Penaeus vannamei) and the tiger prawn (Penaeus monodon), which are extensively cultured in aquaculture systems globally.
Researchers studying these shrimp can leverage the PubCompare.ai platform to access the most accurate and reproducible protocols from literature, preprints, and patents, as well as identify the best products and procedures to streamline their Penaeus studies.
When conducting Penaeus research, scientists may utilize various lab materials and techniques, such as Bovine serum albumin for protein quantification, Brain heart infusion broth for microbial growth, Hydrochloric acid and Acetonitrile for sample preparation, Whatman No. 4 filter paper for filtration, Acetic acid for pH adjustment, the SMARTer RACE kit for gene expression analysis, Glycerol for cryopreservation, the BCA protein assay kit for protein measurement, and TRIzol reagent for RNA extraction.
By integrating these tools and methodologies, researchers can optimize their Penaeus investigations and uncover valuable insights about these economically important crustaceans.