Fusarium oxysporum
This soil-borne fungus can infect the roots and vascular system of host plants, leading to wilting, stunting, and eventual plant death.
Effective control and prevention of Fusarium oxysporum infections remains a critical challenge for agricultural researchers and producers.
Furthering our understanding of this pathogen's biology, epidemiology, and host-pathogen interactions is essential for developing improved disease managemnet strategies.
Most cited protocols related to «Fusarium oxysporum»
A high-throughput DNA isolation protocol [43 ] was adopted to isolate DNA from the leaf tissues in 96-well format. DNA quantification, quality check and normalization to 5 ng/μl were done on agarose gel (0.8%) using lambda DNA standard (MBI Fermentas, USA). DNA isolated for all the 3000 accessions at ICRISAT was supplied to ICARDA for genotyping with 15 SSR markers.
Small subunit (SSU) rRNA gene sequences corresponding to the organisms were retrieved from the local genome database using Infernal [36] (link). In cases where there was more than one SSU rRNA gene sequence in a genome, the longest sequence was chosen. Taxonomic assignment was based on NCBI classification, which was verified by classification of SSU sequences using the SILVA database [37] (link). In addition, habitat and isolation source was either downloaded from the Genomes online database (GOLD, 2012 November 15,
Most recents protocols related to «Fusarium oxysporum»
Biochemical characterization of isolated bacterial strain.
Strains | Biochemical characterization | |||||
---|---|---|---|---|---|---|
Amylase | Catalase | Urease | Citrate test | Methyl red | Voges-Proskauer | |
Pseudomonas sp. | ++ | +++ | – | + | – | – |
Pseudomonas sp. | ++ | ++ | – | ++ | – | – |
Serratia marcescens | ++ | + | + | + | – | + |
Bacillus cereus | ++ | ++ | + | – | – | + |
Ochrobactrum sp. | + | + | – | – | – | + |
Azospirillum brasilensis | + | + | + | + | + | – |
Paenibacillus polymyxa | + | ++ | – | + | – | – |
*Note: In this table “+++”, “++”, “+” and “-”represent the production ability of microbes in high, moderate, low and absent, respectively. All experiment was conducted with 3 replications setup.
Characterization of plant growth promoting biochemical activities of isolated strain.
Strains | Phosphate solubilization (μgml−1) at 3days | IAA production (μg ml−1) at 48 h | Siderophore production | Ammonia production | HCN production | Biocontrol activity | ||
---|---|---|---|---|---|---|---|---|
150 μgml−1 tryptophan | 300 μgml−1 tryptophan | Fusarium oxysporum | Rhizoctonia solani | |||||
Pseudomonas sp | 39.25 ± .66e | 30.05 ± .86f | 34.86 ± .17e | ++ | +++ | ++ | + | ++ |
Pseudomonas sp | 33.02 ± .14c | 18.27 ± .60b | 32.06 ± .05d | + | ++ | + | + | ++ |
Serratia marcescens | 33.30 ± .16c | 23.70 ± .35d | 26.59 ± .07c | + | + | + | – | – |
Bacillus cereus IESDJP-V4 | 37.48 ± .44d | 20.08 ± .05c | 25.23 ± .09b | + | + | + | + | + |
Ochrobactrum sp | 24.76 ± .12b | 25.30 ± .87e | 55.48 ± .08g | + | ++ | + | – | – |
Azospirillum brasilense | 19.12 ± .12a | 40.59 ± 1.18g | 52.08 ± .13f | + | ++ | – | – | – |
Paenibacillus polymyxa | 136.14 ± .10f | 12.56 ± .18a | 23.11 ± .03a | + | + | + | + | + |
Note: The data Values are the mean ± SE, mean values in each column with the same superscript (s) do not differ significantly by Duncan multiple post hoc test (P = 0.05). The sign “+++”, “++”, “+” and “-” represent the production ability of microbes in high, moderate, low and absent, respectively. All experiment was conducted with 3 replications setup.
Pseudomonas sp. IESDJP-V1 showed inhibition zone against Fusarium oxysporum (A) and Rhizoctonia solani (B) on dual media plate of mixture of 50% nutrient agar and 50% Potato dextrose agar
Athelia rolfsii (Curzi) Tu & Kimbrough (provided by the Belgian Co-Ordinated Collections of Microorganisms “BCCM”/Agro-Food and Environmental fungi MUCL 051031) was reactivated on V8 juice agar (200 ml of V8 juice; 3 g of CaCO3; 15 g of agar; 800 ml of distillated water; pH = 7.2). After this step, fungus was cultivated on Oatmeal agar OMA (Per liter: 60 g of oatmeal; 12.5 g of agar, pH = 7.2 ± 0.2) to produce mycelia and sclerotia at 28°C. Inoculated petri dishes were stored at 4°C or collected sclerotia were retained in peptone water (10 g/l of peptone, 5 g/l of NaCl, 1-2 g of tween 80) at the same temperature for the next experiments.
Fusarium oxysporum, Aspergillus niger and Rhizoctonia solani (lab collection) were cultivated on Potato Dextrose Agar PDA (39 g/l, pH = 5.6 ± 0.2; Merck Germany) to produce mycelia. Cultivated fungi were stored at 4°C for subsequent experiments.
Fusarium oxysporum, F. solani, and F. nivale strains used in the present study were isolated from diseased leaves, stems, and roots of ryegrass and from soil samples before being identified. For morphological characterisation, macroscopic traits such as the colony appearance, colour, pigmentation and growth rate were observed on potato dextrose agar (PDA) according to Leslie & Summerell (2006) . Morphological identification was also performed based on the morphological characteristics observed at optical microscope as described by Booth (1971) and Leslie & Summerell (2006) .
Top products related to «Fusarium oxysporum»
More about "Fusarium oxysporum"
This soil-borne fungus can infect the roots and vascular system of host plants, leading to wilting, stunting, and eventual plant death.
Fusarium wilt, as it's commonly known, remains a critical challenge for agricultural researchers and producers.
Effective control and prevention of Fusarium oxysporum infections is essential for protecting crop yields and ensuring food security.
Researchers and scientists are working to deepen their understanding of this pathogen's biology, epidemiology, and host-pathogen interactions, which is crucial for developing improved disease management strategies.
PubCompare.ai is an AI-driven platform that can enhance the reproducibility and accuracy of Fusarium oxysporum research.
The platform helps researchers locate relevant protocols from literature, pre-prints, and patents, and uses AI-driven comparisons to identify the best protocols and products.
By leveraging the power of PubCompare.ai, researchers can improve their research outcomes and experience the difference in their Fusarium oxysporum studies.
In addition to Fusarium oxysporum, other relevant microorganisms like Candida albicans, Pseudomonas aeruginosa, Bacillus subtilis, and Escherichia coli are also of interest in agricultural and medical research.
Potato dextrose agar (PDA) and potato dextrose broth are commonly used meddia for culturing and studying these microbes.
By harnessing the insights from the MeSH term description and the Metadescription, researchers can optimize their Fusarium oxysporum research and make significant strides in developing effective disease management strategies.
The combination of scientific knowledge and the power of AI-driven tools like PubCompare.ai can be a gamechanger in this field.