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Chinchilla

Q: What are the unique adaptations of Chinchillas to their mountain environment?

Chinchillas have evolved several remarkable adaptations to thrive in the harsh Andes mountain environment. These include their ability to conserve water and regulate body temperature. Their dense, plush fur helps insulate them from the cold, and their agile, cloud-like movements allow them to navigate the steep, rocky terrain with ease. Researchers continue to study these fascinating adaptations to better understand Chinchilla biology and behavior.

Q: How can PubCompare.ai help with Chinchilla research?

PubCompare.ai is an AI-driven platform that can optimize Chinchilla research by helping researchers more efficiently screen protocol literature and leverage AI to pinpoint critical insights. The platform's cutting-edge analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the best options for reproducibility and accuracy in their Chinchilla studies. This helps ensure trust in the research findings and accelerates the pace of discovery around these unique rodents.

Q: What are some common challenges in using Chinchillas for research?

Chinchillas can present some unique challenges for researchers, such as their nocturnal nature and sensitivity to environmental conditions. Their small size and delicate physiology also require specialized handling and housing protocols. Researchers must carefully consider factors like diet, temperature, and enrichment to maintain the health and wellbeing of Chinchillas used in studies. PubCompare.ai can help identify the most effective protocols to address these challenges and ensure the ethical and productive use of Chinchillas in research.

Q: Are there different types or variations of Chinchillas?

Yes, there are several recognized subspecies and color variations of Chinchillas. The most common type is the Chinchilla laniger, which is native to the Andes mountains. There are also Chinchilla chinchilla and Chinchilla brevicaudata, which have slightly different physical characteristics and habitat preferences. Additionally, selective breeding has produced a range of color morphs, from the standard gray to white, beige, and even black Chinchillas. Researchers must consider these variations when designing Chinchilla studies to ensure they are using the most appropriate subjects for their specific research goals.

Q: How can Chinchillas be used in biomedical research?

Chinchillas have been the subject of scientific research in a variety of biomedical fields. Their unique physiology and adaptations make them valuable models for studying topics like auditory function, respiratory systems, and even certain genetic disorders. Chinchillas have also been used in research related to the development of new pharmaceutical drugs and treatments. By leveraging the insights gleaned from Chinchilla studies, researchers can gain a better understanding of human and animal health, as well as potential applications for conservation efforts. PubCompare.ai can help idenfity the most relevant and effective Chinchilla research protocols to support these important biomedical investigations.

Chinchilla: A small, nocturnal rodent native to the Andes mountains of South America.
Chinchillas are renowned for their soft, dense fur and playful personalities.
These agile, cloud-like creatures are popular as pets and have also been the subject of scientific research.
Chinchillas are known for their unique adaptations to the harsh mountain environment, including their ability to conserve water and regulate body temperature.
Researchers continue to study these fascinating animals to better understand their biology, behavior, and potential applications in areas like biomedical science.
Discover the power of PubCompare.ai, the AI-driven platform that optimizes Chinchilla research by helping you locate the best protocols from literature, pre-prints, and patents.
Our cutting-edge AI comparisons ensure reproducibility and accuraacy, so you can trust your research findings.
Experence the future of Chinchilla research with PubCompare.ai.

Most cited protocols related to «Chinchilla»

Laser Doppler Vibrometry of Tympanic Membrane

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Publication 2009

Bones Cartilage Chinchilla External Auditory Canals Felidae Head Homo sapiens Light Middle Ear Powder Pulp Canals Saline Solution Sound Speculum Stapes Temporal Bone Transmission, Communicable Disease Tympanic Membrane Ultrasonography Vibration

Vestibular Hair Cell Ablation in Chinchillas

All animal experiments were conducted in accordance with protocols approved by the Johns Hopkins Animal Care and Use Committee. Four adult chinchillas (C. laniger, 450–650g) were anesthetized with ketamine/xylazine IM and then treated bilaterally with 0.5cc intratympanic injections of 26.7 mg/mL gentamicin buffered with sodium bicarbonate to pH 7.0. This regimen ablates 3D aVOR responses to head rotation by destroying Type I vestibular hair cells and denuding Type II vestibular hair cells of their stereocilia while leaving a viable population of Type II hair cell bodies and spontaneously firing ampullary and macular nerve fibers subjacent to the endorgan neuroepithelium (Hirvonen et al 2005 (link); Della Santina et al 2005b , 2007b (link), 2010 (link); Lyford-Pike et al 2007 (link); Fridman et al 2010 (link)).
For animal restraint during testing, a post was affixed to the animal’s skull using dental cement. For prosthetic electrical stimulation, each chinchilla was implanted with four pairs of electrodes. One pair was placed within or near each of the three semicircular canal ampullae, while the last pair served as reference electrodes and was implanted in the neck musculature. Electrodes consisted of Teflon^™ coated 10% iridium/90% platinum wires of diameters ranging from 25um to 125um (Medwire, Sigmund Cohn Corp, Mount Vernon, NY). Distal ends of each wire were stripped 200 μm from the end. Electrode wires of 25 μm diameter were flamed to form a ball on the distal end in order to increase the contact area of the electrode-perilymph interface. This was necessary to avoid electrode corrosion and nerve injury that would otherwise occur during passage of stimulus currents (Robblee and Rose 1990 ).

Hayden R., Sawyer S., Frey E., Mori S., Migliaccio A.A, & Della Santina C.C. (2011). Virtual Labyrinth model of vestibular afferent excitation via implanted electrodes – Validation and application to design of a multichannel vestibular prosthesis. Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 210(3-4), 623-640.

Publication 2011

Adult Animals Auditory Hair Cell Bicarbonate, Sodium Chinchilla Corrosion Cranium Dental Cements Esocidae Gentamicin Hair Cells, Vestibular Head Human Body Injuries Iridium Ketamine Macula Lutea Neck Muscles Nerve Fibers Nervousness Perilymph Platinum Semicircular Canals Stereocilia Stimulations, Electric Teflon Treatment Protocols Xylazine

Evaluating Immune Response and Otitis Media in Chinchilla Model

Blood for serum was collected via cardiac puncture prior to immunization (pre) and one week after receipt of the second immunizing dose (immune) for all animals immunized via a preventative regimen. Animals immunized via a therapeutic regimen were bled prior to NTHI challenge and at study end.
NP lavages were performed on animals immunized via a preventative regimen prior to immunization, one week after receipt of the final dose and on days 3, 7, 10, and 14 after bacterial challenge by passive inhalation of 500 μl sterile pyrogen-free saline as previously described 24 (link).
Video otoscopy (using a 0°, 3-in. probe connected to a digital camera system) to monitor signs of tympanic membrane inflammation and/or presence of fluid within the middle ear space was performed and overall signs of OM were rated on a scale of 0 to 4+ as previously described 7 (link), 24 (link), 31 (link) . Middle ears with a score of ≥ 2.0 were always considered positive for OM as MEF is visible behind the tympanic membrane. Each middle ear was considered independent, and for each cohort, the percentage of middle ears with OM was calculated.
Epitympanic taps to retrieve middle ear effusions were performed on any chinchilla whose tympanic membrane was rated as ≥ 2.5 on a scale of 0 to +4.0. Epitympanic taps were not performed on ears ranked 2.0, due potential for perforation of the tympanic membrane to retrieve the low volume of MEF. NP lavage and middle ear fluids were serially diluted and plated onto chocolate agar supplemented with 15 μg ampicillin/ ml medium or chocolate agar, respectively, to semi-quantitate CFU NTHI per ml fluid type. The mean CFU NTHI/ ml fluid was reported for each cohort.

Novotny L.A., Clements J.D, & Bakaletz L.O. (2011). Transcutaneous immunization as preventative and therapeutic regimens to protect against experimental otitis media due to nontypeable Haemophilus influenzae. Mucosal Immunology, 4(4), 456-467.

Publication 2011

Agar Ampicillin Animals Bacteria Cacao Chinchilla Ear Fingers Heart Inflammation Membrane Potentials Middle Ear Otitis Media with Effusion Otoscopy Punctures Pyrogens Saline Solution Serum Sterility, Reproductive Therapeutics Treatment Protocols Tympanic Membrane Tympanic Membrane Perforation Vaccination

Characterizing NTHi Antigens in Childhood AOM

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Publication 2010

Antigens Child Children's Health Chinchilla Communicative Disorders Diagnosis glycerophosphodiester phosphodiesterase Immunoglobulins Infection Lipoproteins Mucous Membrane Oropharynxs Otitis Media Otoscopy Parenteral Nutrition Pediatricians Proteins Serum Staphylococcal Protein A Strains Tympanostomy Vaccination

Arabidopsis Protoplast Isolation and Bacterial Inoculation

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Publication 2008

Agar Agrobacterium Animals, Transgenic Antibiotics, Antitubercular Arabidopsis Bacteria Bacterial Vaccines BAK1 protein, human Cauliflower Mosaic Virus Chinchilla Cotyledon Epitopes Humidity isolation Light Lycopersicon esculentum Phenotype Plants Plants, Transgenic Protoplasts Seedlings Strains Sucrose Syringes Western Blotting

Most recents protocols related to «Chinchilla»

Comparative Brainstem Histology Across Species

For this review we also examined and photographed archival slides of brainstems from chimpanzees, monkeys, cats and several rodents (chinchilla, rat, guinea pig). Table 2 lists the animal cases. Cresyl and immunostained sections from several species (chimpanzee, monkey, cat, chinchilla, guinea pig, rat) were prepared in the laboratory at the University at Buffalo (Baizer and Baker, 2005 (link), 2006a (link),b (link); Manohar et al., 2012 (link); Baizer et al., 2013a (link),2015 (link); Paolone et al., 2014 (link)). Details of tissue acquisition and histology were given in those publications. In addition, we examined stained sections of chimpanzee, monkey and cat brains that had been prepared in other laboratories (see Table 2).

Baizer J.S, & Witelson S.F. (2023). Comparative analysis of four nuclei in the human brainstem: Individual differences, left-right asymmetry, species differences. Frontiers in Neuroanatomy, 17, 1069210.

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Publication 2023

Animals Brain Brain Stem Buffaloes Cavia Chinchilla Monkeys Pan troglodytes Rodent Tissues

Toxicity and Tolerance of Novel Immunogen

A standard PLVF toxicity study was performed in rabbits. Chinchilla male and female rabbits were purchased from the Federal State Budgetary Institution of Science “Scientific Center for Biomedical Technologies of the Federal Medical and Biological Agency”, Russia. Acute toxicity studies were performed with a single subcutaneous injection of 125 μg/0.5 mL of PLVF (5 male and 5 female rabbits per group, aged 3 months at the start of experiment and weighing 2708 ± 71 g), followed by observation for 14 days. Animals in the control group were injected with 0.5 mL of PBS. Chronic toxicity studies were performed with daily subcutaneous immunization of rabbits with 125 μg/0.5 mL PLVF for a week (5 males and 5 females per group), followed by observation for 7 days. Animals in the control group were injected with 0.5 mL of PBS. Blood samples for hematology and biochemistry were collected before vaccination, and 7 and 14 days after vaccination. On the 14th day after vaccination, necropsy, histological examination of 16 internal organs and tissues of each rabbit, and the blood differential test were performed. The local irritation was studied by histopathological evaluation of the site of repeated subcutaneous injection of the vaccine preparation (macro- and microscopic description of the skin at the injection site).
Tissue samples for histological examination were fixed in 10% neutral buffered formalin, dehydrated in ascending concentrations of alcohol, and embedded in paraffin. Paraffin sections 5 μm thick were cut on a SM 2000R microtome (Leica, Wetzlar, Germany), stained with hematoxylin and eosin, and examined using a DM1000 microscope (Leica, Wetzlar, Germany).
The study of acute and chronic toxicity, as well as the study of the local tolerance of PLVF, was carried out in accordance with the recommendations and the requirements of local legislation (Federal Law of 12.04.10 N 61 “On the Circulation of Medicines”).

Ledov V.A., Golovina M.E., Alkhazova B.I., Lvov V.L., Kovalchuk A.L, & Aparin P.G. (2023). A Pentavalent Shigella flexneri LPS-Based Vaccine Candidate Is Safe and Immunogenic in Animal Models. Vaccines, 11(2), 345.

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Publication 2023

Animals Autopsy Biomedical Technology Biopharmaceuticals BLOOD Chinchilla Eosin Ethanol Females Formalin Hematologic Tests Immune Tolerance Immunization Males Microscopy Microtomy Oryctolagus cuniculus Paraffin Paraffin Embedding Pharmaceutical Preparations Rabbits Skin Subcutaneous Injections Tissues Vaccination Vaccines Vaginal Examination

Chinchilla Ivermectin Study Protocol

This study included 10 adult chinchillas (5 males and 5 females), ranging in age from 1–2 years, which belong to a commercial farm in Beijing, China. No animals participating in the study received any treatment prior to the experiment. Chinchillas were placed in individual cages, within a climate-controlled room temperature maintaining between 21 °C and 23 °C, and they were provided with a commercial pelleted diet (Mazuri^® Chinchilla Diets, Land O’Lakes, Inc., Melrose, MN, USA) with fresh timothy hay and tap water ad libitum. All animals were clinically healthy based on normal physical examination and close observation of food intake and fecal output. The shape and color of all fecal pellets during the experiment were normal, and for fecal samples collected from the 10 chinchillas on day 0 (before ivermectin injection) and day 14 (14 days after ivermectin injection), no gastrointestinal parasites were found in fecal fresh smear and zinc sulfate floating test under microscopic examination. Our study was approved by the animal care and use committee of China Agricultural University and we obtained the informed consent statement from the breeder.

Ma X., Li J., Yang L., Liu H., Zhu Y., Ren H., Yu F, & Liu B. (2023). Short Term Effect of Ivermectin on the Bacterial Microbiota from Fecal Samples in Chinchillas (Chinchilla lanigera). Veterinary Sciences, 10(2), 169.

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Publication 2023

Adult Animals Chinchilla Climate Diet Eating Feces Females Intestinal Diseases, Parasitic Ivermectin Males Microscopy Pellets, Drug Physical Examination Zinc Sulfate

Ivermectin Effects on Chinchilla Microbiome

Within subjects, we performed a before and after study instead of a cross over study given that the necessary washout period of ivermectin in chinchillas is unknown. In this experiment, the 10 chinchillas were injected subcutaneously with ivermectin injection (Ivomec^®, Merial, Ingelheim am Rhein, Germany, 1.0% w/v sterile solution, dilute 10 times by saline) 0.4 mg/kg on day 0.
Fresh fecal samples were collected on day 0 and day 14, divided into day 0 group and day 14 group, respectively. Chinchillas were placed individually into clean cages, and fecal pellets were collected upon evacuation. Following collection, these samples were transferred immediately into sterile cryogenic vials by sterile forceps and stored at −80 °C until microbiota analysis [14 (link)].

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Publication 2023

Chinchilla Feces Forceps Ivermectin Ivomec Microbial Community Pellets, Drug rhein Saline Solution Sterility, Reproductive Technique, Dilution

Positive Selection in Thyroid Genes

We obtained all available coding sequence isoforms from NCBI RefSeq for eight genes known to be involved in thyroid function (Tpo, Tg, Duox1, Duox2, Duoxa1, Duoxa2, Pax8 and Slc5a5), belonging to guinea pig (Cavia porcellus), chinchilla (Chinchilla lanigera), mouse (Mus musculus), rat (Rattus norvegicus) or any species of the Bathyergidae family. Because the proportion of gaps and poorly alignable regions tends to increase with each additional species in the analysis, leading to the exclusion of more positions from the analysis, we made a conscious decision to limit the number of species used. An important reason for this decision was that we tested positive selection in only one species (rather than an entire clade). In this case it is sufficient to identify sequence divergences in the target species that on the one hand represent differences from multiple closely related species (other mole-rat species or guinea pig), while on the other hand the consensus sequence at those positions is conserved over long evolutionary distances (chinchilla, mouse, rat).
We then used the PosiGene pipeline to test these genes for positive selection and identify selected sites on the naked mole-rat branch by applying the branch-site test of positive selection^{31 (link),32 (link)}. Those genes resulting in a false discovery rate (FDR) < 0.1 were further analysed for potential misinterpretation of relaxed selection as positive selection using the RELAX framework^{33 (link)}.

Gerhardt P., Begall S., Frädrich C., Renko K., Hildebrandt T.B., Holtze S., Heinrich A., Sahm A., Meci X., Köhrle J., Rijntjes E, & Henning Y. (2023). Comparative analysis of thyroid hormone systems in rodents with subterranean lifestyle. Scientific Reports, 13, 3122.

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Publication 2023

Biological Evolution Cavia Cavia porcellus Chinchilla Consciousness Consensus Sequence Dual Oxidase 1 Dual Oxidase 2 Genes Mice, House Mole Rats Open Reading Frames PAX8 protein, human Protein Isoforms Rattus norvegicus Thyroid Gland

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The Novex Bis-Tris pre-cast gel system is a laboratory equipment product designed for electrophoresis. It provides pre-cast polyacrylamide gels for protein separation and analysis.

Anti mouse ap and anti rabbit ap secondary antibodies by Merck Group

Anti-mouse-AP and anti-rabbit-AP secondary antibodies are laboratory reagents used in immunoassays and other analytical techniques. They are conjugated with the enzyme alkaline phosphatase (AP), which allows for detection and quantification of target proteins or antigens in samples. These secondary antibodies bind to primary antibodies raised against mouse or rabbit proteins, respectively, enabling signal amplification and visualization of the analyte of interest.

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Protein A-AP secondary antibody is a laboratory reagent used in various immunoassays and immunochemical techniques. It is composed of Protein A, a bacterial protein that binds to the Fc region of immunoglobulins, coupled to alkaline phosphatase (AP), an enzyme that catalyzes a color-producing reaction. This secondary antibody can be used to detect and quantify the presence of primary antibodies in a sample.

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What are the unique adaptations of Chinchillas to their mountain environment?

Chinchillas have evolved several remarkable adaptations to thrive in the harsh Andes mountain environment. These include their ability to conserve water and regulate body temperature. Their dense, plush fur helps insulate them from the cold, and their agile, cloud-like movements allow them to navigate the steep, rocky terrain with ease. Researchers continue to study these fascinating adaptations to better understand Chinchilla biology and behavior.

How can PubCompare.ai help with Chinchilla research?

PubCompare.ai is an AI-driven platform that can optimize Chinchilla research by helping researchers more efficiently screen protocol literature and leverage AI to pinpoint critical insights. The platform's cutting-edge analysis can highlight key differences in protocol effectiveness, enabling researchers to choose the best options for reproducibility and accuracy in their Chinchilla studies. This helps ensure trust in the research findings and accelerates the pace of discovery around these unique rodents.

What are some common challenges in using Chinchillas for research?

Chinchillas can present some unique challenges for researchers, such as their nocturnal nature and sensitivity to environmental conditions. Their small size and delicate physiology also require specialized handling and housing protocols. Researchers must carefully consider factors like diet, temperature, and enrichment to maintain the health and wellbeing of Chinchillas used in studies. PubCompare.ai can help identify the most effective protocols to address these challenges and ensure the ethical and productive use of Chinchillas in research.

Are there different types or variations of Chinchillas?

Yes, there are several recognized subspecies and color variations of Chinchillas. The most common type is the Chinchilla laniger, which is native to the Andes mountains. There are also Chinchilla chinchilla and Chinchilla brevicaudata, which have slightly different physical characteristics and habitat preferences. Additionally, selective breeding has produced a range of color morphs, from the standard gray to white, beige, and even black Chinchillas. Researchers must consider these variations when designing Chinchilla studies to ensure they are using the most appropriate subjects for their specific research goals.

How can Chinchillas be used in biomedical research?

Chinchillas have been the subject of scientific research in a variety of biomedical fields. Their unique physiology and adaptations make them valuable models for studying topics like auditory function, respiratory systems, and even certain genetic disorders. Chinchillas have also been used in research related to the development of new pharmaceutical drugs and treatments. By leveraging the insights gleaned from Chinchilla studies, researchers can gain a better understanding of human and animal health, as well as potential applications for conservation efforts. PubCompare.ai can help idenfity the most relevant and effective Chinchilla research protocols to support these important biomedical investigations.

More about "Chinchilla"

Chinchillas are fascinating small rodents native to the Andes mountains of South America.
These agile, cloud-like creatures are renowned for their soft, dense fur and playful personalities, making them popular as pets.
Chinchillas are known for their unique adaptations to the harsh mountain environment, including their ability to conserve water and regulate body temperature.
Beyond their captivating physical traits, chinchillas have also been the subject of scientific research, with researchers studying their biology and behavior to better understand their potential applications in areas like biomedical science.
When conducting chinchilla research, scientists often utilize various tools and techniques to ensure accurate and reproducible results.
For example, Protein A-HRP, Novex Bis-Tris pre-cast gel systems, and anti-mouse-AP and anti-rabbit-AP secondary antibodies can be used for protein analysis and detection.
Additionally, Protein A-AP secondary antibody and SigmaFAST NBT/BCIP tablets can facilitate colorimetric protein detection.
Peak Scanner software may be employed for data analysis, while JMP Pro 14 can provide advanced statistical capabilities.
Specialized growth media, such as Chocolate agar, can support the cultivation and study of microorganisms relevant to chinchilla research.
Researchers in the field of chinchilla studies may also leverage remote sensing technologies like SPAD-502 to gather valuable data on the animals' natural habitats and behaviors.
Furthermore, the use of GoTaq PCR reaction allows scientists to amplify and analyze genetic material, potentially leading to new insights into chinchilla biology and evolution.
By combining the insights gained from the MeSH term description and the metadescription, along with the incorporation of related tools and techniques, researchers can optimize their chinchilla studies and uncover the full potential of these fascinating creatures.
Experience the future of chinchilla research with the power of PubCompare.ai, the AI-driven platform that helps locate the best protocols from literature, pre-prints, and patents, ensuring reproducibility and accuraccy in your findings.