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Ferrets

Ferrets are small, carnivorous mammals that belong to the Mustelidae family.
They are known for their playful and curious nature, as well as their distinctive masked facial features.
Ferrets are commonly kept as pets and are also used in medical research, particularly for the study of influenza and other respiratory diseases.
These agile and intelligent creatures have a lifespan of 5-10 years and require a specialized diet and environment to thrive.
Ferret research is an important field that can provide valuable insights into human health and disease, and PubCompare.ai is an AI-driven platform that can help optimize this research by identifying the most reproducible and accurate protocols from literature, pre-prints, and patents.
With its advanced AI comparisons, PubCompare.ai can streamline ferret studies and help researchers find the best protocols and products for their work.

Most cited protocols related to «Ferrets»

1
HA-Ferritin Nanoparticle Influenza Vaccine
All genes used for recombinant proteins and pseudoviruses were synthesized using mammalian preferred codons. The HA-ferritin fusion gene was generated by fusing the ectodomain of HA (residues HA1 1-HA2 174, H3 numbering system) to H. pylori ferritin (residues 5–167) with a Ser-Gly-Gly linker. Recombinant proteins were produced by transient transfection of expression vectors in 293F cells (Invitrogen) and purified by chromatography techniques (see Methods for detail). The TIV used in this study were 2006–2007 and 2011–2012 Fluzone® (Sanofi Pasteur). Animal experiments were carried out in accordance with all federal regulations and NIH guidelines. Mice were immunized intramuscularly twice with 0.17 μg (Fig. 2a and b) or 1.67 μg (Fig. 2d) of HA-nanoparticles (HA amount) or matched amount of TIV with or without Ribi adjuvant system (Sigma) or with MF59 (Novartis) at a 3-week interval. Ferrets were immunized intramuscularly with 2.5 μg of HA-nanoparticles or 7.5 μg of TIV with Ribi at weeks 0 and 4. H1N1 virus challenge was performed five weeks after the last immunization with 106.5 50% egg infectious dose (EID50) of 2007 Bris virus via intranasal inoculation. Statistical analyses were performed using Prism 5 (GraphPad Software).
Full Methods and any associated references are available in the online version of the paper.
Kanekiyo M., Wei C.J., Yassine H.M., McTamney P.M., Boyington J.C., Whittle J.R., Rao S.S., Kong W.P., Wang L, & Nabel G.J. (2013). Self-Assembling Influenza Nanoparticle Vaccines Elicit Broadly Neutralizing H1N1 Antibodies. Nature, 499(7456), 102-106.
Publication 2013
Cells Chromatography Codon Ferrets Ferritin Genes Genetic Vectors Helicobacter pylori Infection Influenza A Virus, H1N1 Subtype Mammals MF59 oil emulsion Mus prisma Recombinant Proteins Ribi adjuvant Transients Vaccination Viral Vaccines
2
In Vivo Calcium Imaging Protocols

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Publication 2018
Animals Animals, Laboratory Calcium Ferrets Finches Mice, Laboratory Microscopy Movement Posterior Parietal Cortex Zebras
3
Benchmarking Influenza Antigenic Drift
The benchmark HI dataset is adopted from [8] , and it includes observed HI values from the reactions from H3N2 influenza A viruses against ferret antisera. These viruses were isolated periodically from locations around the world between 1968 and 2003, and the antisera were generated against prototype influenza strains, most of which were selected from these influenza isolates. Both influenza antigen (virus) and antiserum (antibody) can be roughly clustered into eleven groups, HK68, EN72, VI75, TX77, BK79, SI87, BE89, BE92, WU95, SY97 and FU02, which represent the eleven major events of antigenic drifts resulting in a pandemic or an epidemic from 1968 to 2003. For instance, FU02 represents a group of influenza viruses isolated around the year of 2002 with similar antigenic characteristics.
Within this dataset, three types of data points are present: Type I, a regular HI titre; Type II, the value is defined as ‘less than a threshold’, e.g. , where , and this value represents the testing antigen and antiserum do not strongly react with each other; Type III, missing values. Following [8] , we preprocess the HI matrix by normalizing the data entries as follows: each Type I entry with the observed value is transformed to , where is the largest HI value among all observed entries and is the maximum HI value for antiserum ; each Type II entry with value is transformed into ; Type III data are replaced with s, representing the missing values.
Cai Z., Zhang T, & Wan X.F. (2010). A Computational Framework for Influenza Antigenic Cartography. PLoS Computational Biology, 6(10), e1000949.
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Publication 2010
Antigenic Drift Antigens Epidemics Ferrets Immune Sera Immunoglobulins Influenza A virus Pandemics Strains Virus Virus Vaccine, Influenza
4
Ferret Neuronal Imaging Protocols
Ferrets were anesthetized with ketamine and isoflurane (2% for surgery, 0.08−2% during imaging). The training protocol consisted of 5s stimulation followed by 10s interstimulus interval; the protocol continued for 20min followed by 10min of no stimulation and this entire procedure was repeated for several hours. For intrinsic signal imaging experiments, cortex was illuminated with 610-nm light and data was acquired using the Imager 2001/3001 (Optical Imaging Inc) 3 (link). For 2-photon experiments, Oregon Green 488 BAPTA-1 AM (Invitrogen) was pressure injected into cortex and changes in calcium fluorescence were monitored with a 2-photon microscope (Prairie Technologies) driven by a mode-locked laser (810nm, Coherent). Stimulation and analysis were performed using custom software for Matlab (Mathworks). See Supplementary Methods for details.
Li Y., Van Hooser S.D., Mazurek M., White L.E, & Fitzpatrick D. (2008). Experience with moving visual stimuli drives the early development of cortical direction selectivity. Nature, 456(7224), 952-956.
Publication 2008
Calcium Cortex, Cerebral Ferrets Fluorescence Isoflurane Ketamine Light Microscopy Operative Surgical Procedures Oregon green 488 BAPTA-1 Pressure
5
Spatial Cue Weighting in Monaural Hearing
Twenty-one ferrets were used in this study, of which ten were raised with an earplug while the remainder were raised normally. The left ear of the juvenile-plugged ferrets was first fitted with an earplug (EAR Classic) between postnatal day 25 (P25) and 29, shortly after the age of hearing onset in this species [45 (link)], and was thereafter monitored routinely and replaced as necessary (see Supplemental Experimental Procedures). All procedures were performed under licenses granted by the UK Home Office and met with ethical standards approved by the University of Oxford.
The animals were trained from ∼P150 onward to approach the location of 200 ms broadband noise bursts with either flattened or randomized spectra in order to receive a water reward. Stimuli were presented from one of 12 speakers positioned in the horizontal plane. Reverse correlation maps were constructed from behavioral responses to randomized-spectrum stimuli. A simulated version of this task was also used to assess the performance of monaural and binaural models of sound localization (see Supplemental Experimental Procedures).
Recordings were made under medetomidine/ketamine anesthesia from A1 units in response to VAS stimuli generated from acoustical measurements in each animal [23 (link)]. These stimuli recreated the acoustical conditions associated with either normal hearing or an earplug in the left ear and were used to manipulate individual spatial cues independently of one another (see Supplemental Experimental Procedures). We calculated the mutual information (MI) between the spike counts within a window spanning the response of each acoustically driven unit (juvenile-plugged ferrets, n = 260; controls, n = 147) and the virtual azimuth corresponding to each of the available spatial cues. In the case of spectral cues, this was done by collapsing the data across azimuth for the other ear. Estimates of MI were used to calculate a weighting index (WI), which compared the MI values obtained using the spatial cues provided by the nonoccluded right ear (MIR) with the sum of the MI values obtained using the other available spatial cues (MIother): WI=MIR−MIotherMIR+MIother.
Keating P., Dahmen J.C, & King A.J. (2013). Context-Specific Reweighting of Auditory Spatial Cues following Altered Experience during Development. Current Biology, 23(14), 1291-1299.
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Publication 2013
Acoustics Anesthesia Animals Earplugs Elp1 protein, human Ferrets Ketamine Medetomidine Microtubule-Associated Proteins Sound Localization

Most recents protocols related to «Ferrets»

1
Functional Ultrasound Imaging of the Ferret Brain

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Publication 2023
Blood Circulation Brain Cluttering Ferrets Maritally Unattached Reading Frames Tissues Transducers
2
Functional Ultrasound Imaging of Visual Cortex

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Publication 2023
Agar Cortex, Cerebral Darkness ECHO protocol Ferrets Pulse Rate Radionuclide Imaging Reading Frames Sinusoidal Beds Transducers Transmission, Communicable Disease Ultrasonics
3
Visualizing Ferret Brain Cytoarchitecture

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Publication 2023
Animals Brain Cortex, Cerebral Ferrets Microscopy Microtomy Oxidase, Cytochrome-c Tissues White Matter
4
Consistent Activation Patterns in Functional Ultrasound

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Publication 2023
Ferrets
5
Neuroimaging of Anesthetized Ferrets

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Publication 2023
Agar Anesthesia Anesthetics Animals Bone Screws Brain Cerebrospinal Fluid Cortex, Cerebral Craniotomy Cranium Dehydration Dura Mater Eye Movements Ferrets Glucose Isoflurane Ketamine Lactated Ringer's Solution Operative Surgical Procedures Oxide, Nitrous Oxygen Pentobarbital Sodium physiology Punctures Rate, Heart Reading Frames Respiratory Rate Rocuronium Bromide Saline Solution Saturation of Peripheral Oxygen Scalp Temporal Muscle Tissues Trachea Tracheostomy Visual Cortex Xylazine

Top products related to «Ferrets»

1
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
2
Receptor destroying enzyme by Denka Seiken
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The receptor-destroying enzyme is a laboratory tool used to remove or inactivate specific receptors from cell surfaces. It functions by cleaving or modifying the receptor molecules, altering their structure and preventing their normal interactions. This enzyme is a valuable instrument for researchers studying receptor-mediated cellular processes and signaling pathways.
3
Type iiia by Merck Group
Type IIIa is a specialized laboratory equipment designed for precise and accurate measurements. It is intended for use in controlled environments, such as research laboratories or quality control settings. The core function of this product is to provide reliable and consistent data collection capabilities for a variety of scientific applications.
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The CUY650P5 is a laboratory equipment product designed for scientific research applications. It serves as a platform for conducting various experiments and analyses. The core function of the CUY650P5 is to provide a controlled environment for sample preparation, observation, and data collection.
5
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The QIAamp Viral RNA Mini Kit is a laboratory equipment designed for the extraction and purification of viral RNA from various sample types. It utilizes a silica-based membrane technology to efficiently capture and isolate viral RNA, which can then be used for downstream applications such as RT-PCR analysis.
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Laboratory Feline Diet 5003 is a complete and balanced diet formulated for laboratory cats. It provides the necessary nutrients to support the health and well-being of cats in a laboratory setting.
7
High density ferret diet 5li4 by LabDiet
High Density Ferret Diet 5LI4 is a complete and balanced laboratory animal diet formulated to meet the nutritional requirements of ferrets. It is designed to provide the necessary nutrients for the overall health and well-being of ferrets in a laboratory setting.
8
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

More about "Ferrets"

Ferrets are fascinating small carnivores that belong to the Mustelidae family.
These agile, intelligent creatures are known for their playful and curious nature, as well as their distinctive masked facial features.
Ferrets are commonly kept as pets, but they also play a crucial role in medical research, particularly in the study of influenza and other respiratory diseases.
Ferret research is an important field that can provide valuable insights into human health and disease.
Researchers often use specialized diets like the Laboratory Feline Diet 5003 or the High Density Ferret Diet 5LI4 to ensure their ferrets receive the proper nutrition.
Additionally, researchers may utilize products like the RNeasy Mini Kit, QIAamp Viral RNA Mini Kit, and Opti-MEM to extract and study genetic material from ferret samples.
One key tool that can help optimize ferret research is PubCompare.ai, an AI-driven platform that can identify the most reproducible and accurate protocols from literature, pre-prints, and patents.
With its advanced AI comparisons, PubCompare.ai can help researchers find the best protocols and products for their ferret studies, streamlining the research process.
Ferrets are also known to secrete a receptor-destroying enzyme (RDE II), which can be used in various assays and experiments.
Additionally, researchers may use the Type IIIa enzyme, which is a penicillin-resistant variant, in their ferret studies.
By incorporating these insights and related terms, researchers can enhance their understanding of ferrets and improve the overall quality and efficiency of their studies.
PubCompare.ai is the ultimate tool for ferret researchers, helping them navigate the complexities of this important field of study.