Log In Sign up
The largest database of trusted experimental protocols
> Living Beings > Mammal > Kunming mice

Kunming mice

Kunming mice are a widely used inbred mouse strain originating from China.
These mice are known for their genetic homogeneity, making them a valuable model for biomedical research, including studies on immunology, neuroscience, and cancer.
Kunming mice demonstrate high reproductive capacity, robust growth, and adaptability to various experimental conditions.
Researchers leverage the consistency and reliability of Kunming mice to enhance the reproducibility and accuracy of their studies, particurally in the fields of toxicology, pharmacology, and infectious disease research.

Most cited protocols related to «Kunming mice»

1
Kunming Mouse Husbandry Protocol
Cited 11 times
A total of 12 specific pathogen-free (SPF) Kunming mice (3 weeks of age, half male, half female) were purchased from Hunan Slike Jingda Laboratory Animal Co., Ltd. and fed in a regulated barrier system with light and dark cycles of 12 h, 23–25°C, and 50–70% relative humidity. After 1 week of adaptive feeding, animals were divided into male and female groups and fed for 8 weeks. Mice were fed by the Animal Experiment Center of Hunan University of Chinese Medicine with nutritional standards in line with GB/14924.3 and sanitation standards in line with GB/T149.24.2 to support their growth and reproduction. All animal experiments were licensed by the Animal Experiment of Hunan University of Chinese Medicine (Changsha, China), and the protocol was approved by the Animal Ethics Committee of Hunan University of Chinese Medicine [Facility use permit number: SYXK (Xiang) 2019-0009].
Wu Y., Peng X., Li X., Li D., Tan Z, & Yu R. (2022). Sex hormones influence the intestinal microbiota composition in mice. Frontiers in Microbiology, 13, 964847.
Publication 2022
Acclimatization Animal Ethics Committees Animals Animals, Laboratory Chinese Females Humidity Kunming mice Light Males Mice, House Pharmaceutical Preparations Reproduction Specific Pathogen Free
2
Isolation and Culture of FMDV Strains
Cited 9 times
BHK-21 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Hyclone). Goat fibroblasts were kindly provided by Prof. Baohua Ma (Northwest Agriculture & Forestry University) and cultured in DMEM/F12 nutrient mixture (Gibco) (containing 1.5 g/L sodium bicarbonate) supplemented with 10% FBS. All cell lines were incubated at 37°C with 5% CO2. Kunming White suckling mice, 2–3 days old and weighing 3–4 g, were purchased from Lanzhou Institute of Biological Products. Five FMDV isolates, O/Tibet/China/1/99 [PanAsia-1 strain of ME-SA (Middle East-South Asia) topotype, AF506822], O/HN/CHA/93 (vaccine strain of Cathay topotype) [67 (link)], O/CHN/Mya98/33-P [Mya98 strain of SEA (South-East Asia) topotype, JQ973889], and AF72 (vaccine strain of Asia topotype) [68 (link)], Asia 1/Jiangsu/China/2005 (vaccine strain of SEA topotype, EF149009), were preserved and provided by OIE/National Foot-and-Mouth Disease Reference Laboratory of China.
Chang Y., Dou Y., Bao H., Luo X., Liu X., Mu K., Liu Z., Liu X, & Cai X. (2014). Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo. Virology Journal, 11, 1.
Publication 2014
Bicarbonate, Sodium Biological Products Cell Lines Cells Eagle Fibroblasts Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Goat Kunming mice Nutrients Strains Vaccines
3
Kunming Mice High-Fat Diet Study
Cited 5 times
Three‐ to four‐week‐old male Kunming mice were purchased from Dashuo Animal Center (Chengdu, China) and housed under specific pathogen‐free conditions. The animals were maintained according to specific protocols, and all euthanasia procedures were approved by the Guidelines for the Care and Use of Laboratory Animals and the Ethics Committee of Sichuan Agricultural University (Ya'an, China). The mice received either a normal diet or a high‐fat diet for 8 weeks. During the experiment, food and water were supplied ad libitum, except during 8‐h food deprivation periods before blood draws.
Wan T., Yuan G., Ren Y., Zuo Z., Wang Z., Jia Y., Cui H., Peng X., Fang J., Deng J., Yu S., Hu Y., Shen L., Ma X., Wang Y, & Ren Z. (2016). Diet‐induced obese mice exhibit altered immune responses to acute lung injury induced by Escherichia coli. Obesity (Silver Spring, Md.), 24(10), 2101-2110.
Publication 2016
Animals Animals, Laboratory Diet, High-Fat Ethics Committees Euthanasia Food Kunming mice Males Mice, House Phlebotomy Specific Pathogen Free Therapy, Diet
4
Generating Transgenic P. yoelii Parasites
Cited 5 times
All transgenic P. yoelii parasites were generated from P. yoelii 17XNL strain. The parasites were propagated in Kunming (KM) outbred mice purchased from the Animal Care Center of Xiamen University. All mouse experiments were performed in accordance with approved protocols (XMULAC20140004) by the Committee for Care and Use of Laboratory Animals at the School of Life Sciences of Xiamen University. The procedures for parasite transfection, selection, and cloning were described previously (26 (link)) and illustrated in Fig. S7 in the supplemental material. Briefly, parasites were electroporated with purified circular plasmid DNA. Transfected parasites were intravenously injected into new mice, and parasites were selected with pyrimethamine (Pyr) in drinking water 1 day after injection. Parasites with transfected plasmids usually appear 5 to 7 days after injection. In allelic replacement experiments, parasite-infected RBCs were transferred to new mice when the parasitemia declined to ~0.5%.
Zhang C., Xiao B., Jiang Y., Zhao Y., Li Z., Gao H., Ling Y., Wei J., Li S., Lu M., Su X.Z., Cui H, & Yuan J. (2014). Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System. mBio, 5(4), e01414-14.
Publication 2014
Alleles Animals Animals, Laboratory Animals, Transgenic DNA, Circular Erythrocytes Kunming mice Mice, Laboratory Parasitemia Parasites Plasmids Pyrimethamine Strains Transfection
5
Toxoplasma gondii Infection in Mice
Cited 4 times
Six- to eight-week-old specific pathogen-free female Kunming mice were purchased from Zhejiang Laboratory Animal Center, Hangzhou, China. All animals were maintained in strict accordance with the Good Animal Practice requirements of the Animal Ethics Procedures and Guidelines of the People’s Republic of China. Animal experiments were approved by the ethics committee of Ningbo University (permission: SYXK(ZHE)2013-0191).
Tachyzoites of the T. gondii RH strain (type I) were used for this study, and were maintained in our laboratory and prepared from human foreskin fibroblast (HFF) cells. HFF cells were cultured with Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA). The obtained tachyzoites were used for total RNA extraction (RNApre Pure Tissue Kit, Sangon Biotech, China) and the preparation of Toxoplasma lysate antigen (TLA), as described in our previous studies [17 (link)]. The tissue cysts of the low-virulence PRU strain were propagated and harvested, as described in our previous studies [4 (link)], and were used for mice challenge.
Zhu Y.C., He Y., Liu J.F, & Chen J. (2020). Adjuvantic cytokine IL-33 improves the protective immunity of cocktailed DNA vaccine of ROP5 and ROP18 against toxoplasma gondii infection in mice. Parasite, 27, 26.
Publication 2020
Animals Animals, Laboratory Antigens Culture Media Cyst Eagle Ethics Committees Females Fetal Bovine Serum Fibroblasts Foreskin Homo sapiens Kunming mice Mus Specific Pathogen Free Strains Tissues Toxoplasma Virulence

Most recents protocols related to «Kunming mice»

1
Evaluating RGO's Therapeutic Effects on LPS-Induced Intestinal Inflammation in Mice
We purchased 36 KM mice from Henan Scribes Biotechnology Co., Ltd. The mice were fed for 7 days before the experiment to acclimate to the environment. The mice were randomly divided into six groups, namely normal (N), model (M), treatment (T), RGO low dose (RL, 0.25 g/kg), RGO medium dose (RM, 0.5 g/kg), and RGO high dose (RH, 1 g/kg) groups. Six mice were in each group, and the test period was 21 days. Except for the normal group, on the 9th, 13th, 17th, and 21st days, 0.2 mL of normal saline was injected intraperitoneally. 0.2 mL of 1 mg/kg LPS was injected intraperitoneally in all other groups to develop mice models of intestinal inflammation and barrier injury (14 (link), 15 (link)). From the first day of the test, mice in RL, RM, and RH groups were provided 0.2 mL of RGO solution by gavage once a day for 21 days. The mice in the N, M, and T groups were gavaged with an equal volume of normal saline daily. After each intraperitoneal LPS injection, the mice in the T group were gavaged with 0.2 mL dexamethasone solution at 0.5 mg/kg dose 30 min. All the groups were fed adequate food and free to drink water. The weight of mice was determined on the 1st, 7th, 14th, and 21st days of the test. Additionally, the food intake, fecal properties, and health status of mice were observed and recorded daily.
Li X., Gui R., Wang X., Ning E., Zhang L., Fan Y., Chen L., Yu L., Zhu J., Li Z., Wei L., Wang W., Li Z., Wei Y, & Wang X. (2023). Oligosaccharides isolated from Rehmannia glutinosa protect LPS-induced intestinal inflammation and barrier injury in mice. Frontiers in Nutrition, 10, 1139006.
Publication 2023
Dexamethasone Eating Feces Food Inflammation Injections, Intraperitoneal Injuries Intestines Kunming mice Mice, House Normal Saline Sensitivity Training Groups
2
Kunming Mice Welfare Protocol
The experimental animals were Kunming mice (weighting 22–25 g, half male and female) provided by the Laboratory Animal Centre, Guangzhou University of Chinese Medicine, Guangzhou, China (approval number: 44007200079662). Mice were cared under the standard laboratory conditions, which is temperature 22°C ± 2°C, humidity 50% ± 10%, and 12 h dark-light cycle. And they were allowed to freely consume sterilized water and standard chow. All experimental procedures were approved by the Animal Ethics Committee of Guangzhou University of Chinese Medicine.
Zheng X., Mai L., Xu Y., Wu M., Chen L., Chen B., Su Z., Chen J., Chen H., Lai Z, & Xie Y. (2023). Brucea javanica oil alleviates intestinal mucosal injury induced by chemotherapeutic agent 5-fluorouracil in mice. Frontiers in Pharmacology, 14, 1136076.
Publication 2023
Animal Ethics Committees Animals, Laboratory Chinese Females Humidity Kunming mice Males Mice, House Pharmaceutical Preparations
3
5-FU-Induced Diarrhea in Mice: A Comparative Study
Kunming mice were randomly assigned into six groups (n = 10): normal group; 5-FU group (5-FU, 60 mg/kg/day for 5 days, intraperitoneal injections); LO group (loperamide; 4 mg/kg/day for 7 days, oral administration); BJO group (0.125 (BJOL), 0.250 (BJOM), 0.500 (BJOH) g/kg/day for 7 days, oral administration). According to previous research (Zheng et al., 2019 (link)), 5-FU intraperitoneal injection (from day 1 to day 5); 30 min later, oral administration of BJO and LO was carried out (from day 1to day 7). The normal group mice were injected with physiological saline and orally received blank emulsion. The 5-FU group mice were injected with 5-FU and orally given blank emulsion. Body weight, stool consistency, food intake, and general appearance of mice were recorded daily. Diarrhea was measured using the mean scores. As shown in Table 1, the severity was quantified according to a previously described procedure (Huang et al., 2009 (link)). After the 7-day treatment, the blood was obtained after enucleation of eyeball, and mice were sacrificed by cervical dislocation. After 2 h, blood was centrifuged for 15 min at 3500 rpm and 4°C in refrigerate centrifuge, and serum was collected.
Zheng X., Mai L., Xu Y., Wu M., Chen L., Chen B., Su Z., Chen J., Chen H., Lai Z, & Xie Y. (2023). Brucea javanica oil alleviates intestinal mucosal injury induced by chemotherapeutic agent 5-fluorouracil in mice. Frontiers in Pharmacology, 14, 1136076.
Publication 2023
Administration, Oral BLOOD Body Weight Diarrhea Eating Emulsions Eye Enucleation Feces Injections, Intraperitoneal Joint Dislocations Kunming mice Loperamide Mice, House Neck physiology Saline Solution Serum
4
Ethical Animal Study of Kunming Mice

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Animal Ethics Committees Animals Animals, Laboratory Body Weight Food Humidity Hypomenorrhea Kunming mice Light Males Mice, House Specific Pathogen Free
5
Hyperuricemia Mouse Model and LPP Treatment
Male Kunming mice (6-week-old, 25 ± 2 g) were purchased from the Chinese Beijing Vital River Laboratory Animal Technology Co., Ltd. All mice were housed in the Animal Center of China CDC Mice with temperature at 24–26°C, humidity at 40–60%, and a 12 h light–dark cycle. Mice had free access to the standard commercial mouse food and water. After one-week adaption, mice were randomly divided into three groups (n = 8/group): negative control group (NC), hyperuricemic group (HUA), and LPP-treated group (LPP). The HUA mouse model was established according to literature with slight modification (18 (link), 21 (link)–26 (link)). Briefly, potassium oxonate (Sigma-Aldrich, MA, United States,) and adenine (Sigma-Aldrich, MA, United States) were suspended in 0.5% Carboxymethylcellulose sodium (CMC-Na) solution. Mice in HUA and LPP groups were daily administered with 0.3 ml solution of potassium oxonate (PO, 250 mg/kg) and adenine (75 mg/kg) by gavage, and NC group mice were given the equal volume of 0.5% CMC-Na solution for 14 days. Four hours after the treatment of PO and adenine, mice in LPP group were orally administered with 1 × 109 CFU LPP in 0.3 ml PBS and mice in NC and HUA groups were given the equal volume of sterile PBS. After 14-day of daily administration of LPP or PBS, mice were sacrificed and body weight was recorded. Blood was collected from the eye vein. To acquire the serum, the collected blood samples were left undisturbed for 30 min then centrifuged at 5000 xg for 20 min. The kidney, liver, ileum, colon, and cecal content were also collected. All samples were stored at −80°C until analysis, except for the samples used for histopathology and RNA sequencing, which were kept in 4% paraformaldehyde and RNAlater (Thermo Fisher Scientific, Waltham, MA, United States) respectively.
Wang Z., Song L., Li X., Xiao Y., Huang Y., Zhang Y., Li J., Li M, & Ren Z. (2023). Lactiplantibacillus pentosus P2020 protects the hyperuricemia and renal inflammation in mice. Frontiers in Nutrition, 10, 1094483.
Publication 2023
Acclimatization Adenine Administration, Oral Aftercare Animals Animals, Laboratory BLOOD Body Weight Cecum Chinese Colon Food Humidity Hyperuricemia Ileum Kidney Kunming mice Liver Males Mice, House paraform potassium oxonate Rivers Serum Sodium Carboxymethylcellulose Specimen Collection Sterility, Reproductive Tube Feeding Veins

Top products related to «Kunming mice»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Kunming mice by Charles River Laboratories
Sourced in China
Kunming mice are an inbred strain of mice developed in China. They are commonly used in biomedical research as an animal model.
3
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
4
Male kunming mice by Charles River Laboratories
Sourced in China
Male Kunming mice are a commonly used rodent model in research. They are a strain of mice that are bred and maintained at Charles River Laboratories. These mice have a genetically uniform background and are available in various age ranges to suit different research needs.
5
Km mice by Charles River Laboratories
Sourced in China
The KM mice are a laboratory mouse model developed and distributed by Charles River Laboratories. The KM mice are a genetically modified strain primarily used for research purposes.
6
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
7
Rpmi 1640 medium by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Japan, France, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Israel, Sweden, Denmark, Macao, Brazil, Ireland, India, Austria, Netherlands, Holy See (Vatican City State), Poland, Norway, Cameroon, Hong Kong, Morocco, Singapore, Thailand, Argentina, Taiwan, Province of China, Palestine, State of, Finland, Colombia, United Arab Emirates
RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
8
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
9
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
10
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

More about "Kunming mice"

Kunming mice, also known as KM mice, are a widely used inbred mouse strain originating from China.
These genetically homogeneous mice are highly valued in biomedical research, particularly in the fields of immunology, neuroscience, and cancer studies.
Kunming mice demonstrate exceptional reproductive capacity, robust growth, and adaptability to various experimental conditions, making them a reliable and consistent model for researchers.
Leveraging the consistency and reliability of Kunming mice is crucial for enhancing the reproducibility and accuracy of research studies, especially in toxicology, pharmacology, and infectious disease research.
Researchers can utilize platforms like PubCompare.ai, an AI-driven tool, to easily locate protocols from literature, preprints, and patents, and to identify the best protocols and products through AI-driven comparisons.
When working with Kunming mice, researchers often utilize FBS (Fetal Bovine Serum), DMEM (Dulbecco's Modified Eagle Medium), RPMI 1640 medium, and reagents like TRIzol for cell culture and molecular biology experiments.
The use of antibiotics such as Penicillin and Streptomycin is also common to prevent bacterial contamination in cell and tissue cultures.
By leveraging the genetic homogeneity and consistent performance of Kunming mice, researchers can enhance the reproducibility and accuracy of their studies, leading to more reliable and impactful research outcomes in a variety of biomedical fields.