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Mice, Inbred ICR

Mice, Inbred ICR: A strain of albino mice that are widely used in biomedical research.
These mice are geenetically homogenius and have a consistent phenotype, making them a valuable model for studying a variety of diseases and conditions.
PubCompare.ai can help researchers identify the best protocols and products for working with Mice, Inbred ICR, enhancing reproducibiltiy and accuracy in their studies.

Most cited protocols related to «Mice, Inbred ICR»

1
CRISPR-Cas9 Genome Editing in Mice
Cited 79 times

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Publication 2013
2',5'-oligoadenylate Amino Acids Anemia, Diamond-Blackfan, 2 Animals Blastocyst Cytoplasm Donors Embryo Females Males Mice, House Mice, Inbred ICR Mothers Oviducts RNA, Messenger Strains Tissue Donors Uterus Zygote
2
CRISPR-Mediated Genetic Modification in Mice
Cited 29 times

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Publication 2013
2',5'-oligoadenylate Amino Acids Anemia, Diamond-Blackfan, 2 Animals Blastocyst Cloning Vectors Cytoplasm Donors Embryo Females Males Mice, House Mice, Inbred ICR Mothers Oviducts Plasmids RNA, Messenger Strains Uterus XXX syndrome Zygote
3
CRISPR-Mediated Knock-In and Knockout Mice
Cited 24 times
For knockout mouse production, Cas9 mRNAs and Actb-sgRNA were diluted and mixed in 0.1 TE buffer (10 mM Tris-HCl, 0.1 mM EDTA (pH 8.0)) to a working concentration of 50 and 20 ng/μl, respectively. One-cell-stage zygotes were obtained by mating of BDF1 males and females (CLEA Japan, Meguro, Tokyo, Japan), and then frozen and stored until use. The mixture of Cas9 mRNAs and Actb-sgRNA was injected into the cytoplasm using a micromanipulator and microscope (Leica, Wetzlar, Germany) and injector (Eppendorf, Hauppauge, NY, USA). After incubation at 37°C for 24 hours, two-cell-stage embryos were transferred into pseudopregnant ICR female mice (CLEA Japan).
For knock-in mouse production by RNA injection, Cas9 mRNAs, Actb-sgRNA, and pActb-TetO-FLEX-EGFP-polyA were diluted and mixed in 0.1 TE buffer to a working concentration of 5, 2.5, and 10 ng/μl, respectively, as previously described [5 (link)]. The injection mixture was injected into pronuclei of one-cell-stage zygotes.
For knock-in mouse production by protein injection, Cas9 proteins, Actb-sgRNA, or Actb-crRNA and tracrRNA, and pActb-TetO-FLEX-EGFP-polyA were diluted and mixed in 0.1 TE buffer to a working concentration of 30 or 100 ng/μl, 2.5 or 25 ng/μl, or 0.061 or 0.61 pmol/μl, and 10 ng/μl, respectively. The mixture was incubated at 37°C for at least 15 minutes, and then injected into pronuclei of one-cell-stage zygotes.
Aida T., Chiyo K., Usami T., Ishikubo H., Imahashi R., Wada Y., Tanaka K.F., Sakuma T., Yamamoto T, & Tanaka K. (2015). Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice. Genome Biology, 16(1), 87.
Publication 2015
Buffers Cells CRISPR-Associated Protein 9 crRNA, Transactivating Cytoplasm Edetic Acid Embryo Females Freezing Males Mice, Inbred ICR Mice, Knockout Microscopy Mus Poly A Proteins RNA, CRISPR Guide RNA, Messenger Tromethamine Zygote
4
Generation of YapS112A Transgenic Mice
Cited 22 times
Transgenic mice were generated by pronuclear injection according to standard procedures. A full-length mouse complementary DNA (cDNA) encoding YapS112A with a FLAG epitope tag was cloned 3′ to a 5.5-kb segment of the βMHC promoter and 5′ to a 0.6-kb polyadenylation signal from the human growth hormone gene. The transgene was linearized with Not I to remove vector sequence, injected into fertilized oocytes from B6C3F1 female mice, and implanted into pseudopregnant ICR mice.
Xin M., Kim Y., Sutherland L.B., Qi X., McAnally J., Schwartz R.J., Richardson J.A., Bassel-Duby R, & Olson E.N. (2011). Regulation of Insulin-Like Growth Factor Signaling by Yap Governs Cardiomyocyte Proliferation and Embryonic Heart Size. Science signaling, 4(196), ra70.
Publication 2011
Cloning Vectors DNA, Complementary Epitopes Females Genes Human Growth Hormone Mice, Inbred ICR Mice, Laboratory Mice, Transgenic Ovum Polyadenylation Transgenes
5
Spinal Cord ScRNA-seq Analysis
Cited 21 times

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Publication 2018
Antibodies Buffers Cell Nucleus Females Formalin In Situ Hybridization Lumbar Cord Males Mice, House Mice, Inbred ICR sodium lauroyl sarcosinate Specimen Handling

Most recents protocols related to «Mice, Inbred ICR»

1
Acclimation and Housing of Female ICR Mice
Not available on PMC !

Example 6

SPF female ICR mice were obtained at 3 weeks of age from Taconic Farms (Hudson, NY), and used for the experiments after one-week acclimation. Mice were housed at the Isolation Unit of the Central Animal Facility (University of Guelph) in a temperature controlled environment with a 12 h light/dark cycle. Animal care was provided in accordance with the animal utilization protocol no. 04R030 (University of Guelph) and the Guide to the Care and Use of Experimental Animals (1). Mice were fed sterilized solid rodent chow and water. When needed, water was supplemented with Amp and Km at a concentration of 400 mg L−1 and 200 mg L−1, respectively. Each mouse was assessed daily for weight, body temperature, signs of dehydration, posture and alertness.

US11857581B2. Antiviral methods and compositions comprising probiotic bacterial molecules (2024-01-02). MicroSintesis Inc. [CA]. Inventors: Mansel Griffiths [CA].
Patent 2024
Acclimatization Animals Body Temperature Dehydration Females isolation Mice, House Mice, Inbred ICR Rodent
2
GR Compounds' Effects on Mouse Blood Glucose
Not available on PMC !

Example 26

33 ICR mice were randomly divided into 11 groups, i.e. normal saline group, 1.8 mg/kg dexamethasone acetate group (Dex), CK, IB, IC, ID, IVA, IH, IJ, IK and IL were respectively given 225 mg/kg. The mice were intragastric administration for 6 consecutive days, fasting was started at about 8:00 in the morning on the sixth day, and blood glucose in caudal vein was measured at about 4:00 in the next day.

TABLE 5
Blood glucose data of GR series compounds
GroupBlood glucose (mmol/L)
Blank group3.05 ± 0.11
Dex 5.78 ± 0.36**
CK3.18 ± 0.28
IB3.10 ± 0.19
IC3.25 ± 0.26
ID2.79 ± 0.56
IVA3.02 ± 0.23
IH2.98 ± 0.37
IJ3.11 ± 0.43
IK3.09 ± 0.28
IL3.03 ± 0.21
Note:
compared to blank group,
* P < 0.05,
**P < 0.01

Blood glucose data showed that compared with the blank control group, dexamethasone could increase blood glucose in mice, while no blood glucose related changes were caused by CK and GR derivatives.

US11866458B2. Panaxadiol glycoside derivative and preparation method and application thereof (2024-01-09). SUZHOU JI ER BIOLOGICAL MEDICINE CO., LTD. [CN]. Inventors: Quanhai Liu [CN], Jun Zhang [CN].
Patent 2024
Blood Glucose derivatives Dexamethasone dexamethasone acetate IL21 protein, human Mice, House Mice, Inbred ICR Normal Saline Veins
3
Isolation and Treatment of Striatal Neurons
Primary striatal neurons were isolated from ICR mice at embryonic day 17 as previously described (Fitting et al., 2014 (link); Zou et al., 2011 (link)). Briefly, striatal tissues were dissected, minced, and incubated for 30 min at 37oC in neurobasal medium (NBM) containing 0.015 mg/mL DNase and 2.5 mg/ml trypsin. NBM was supplemented with 25 mM glutamate, 0.5 mM glutamine, B27 (Invitrogen), and an antibiotic-antimycotic solution (Sigma). Cells were then triturated, filtered (2×) through a 70 μm-diameter pore membrane, and seeded into six-well plates (15   ×   105 neurons/well) pre-coated with poly-L-lysine. Cells were maintained in NBM supplemented at decreasing concentrations of glutamate (days 1–4, 25 mM; days 5–6, 12.5 mM; days 7–11, 0 mM) at 37oC in a 5% CO2 environment until they matured (at 11 days). Based on our findings that TDP-43 phosphorylation was greatest following co-exposure to morphine and Tat in murine brain tissues, and to optimize detecting alterations in TDP-43 phosphorylation, we co-exposed cells to Tat and morphine to verify the involvement of CK2 in the pathologic TDP-43 phosphorylation. Cells were either treated with vehicle (DNase/RNase-free non-pyrogenic Ultrapure water), co-treated with Tat (100 nM) and morphine (500 nM), or a combination of Tat, morphine, and 0.5, 1, or 2 µM concentrations of the highly selective CK2 antagonist CX-4945 (#A11060, AdooQ Bioscience, Irvine, CA). CX-4945 (Silmitasertib) was granted Orphan Drug Designation by the US FDA in December 2021 for the treatment of medulloblastoma and potentially other rare diseases targeting CK2. Vehicle-treated cells served as controls. The selected concentrations of Tat and morphine do not affect cell viability within 24 h (Zou et al., 2011 (link)). The doses of CX-4945 selected for testing are those previously determined to reduce pro-inflammatory mediators to baseline levels in immune cells (Jang et al., 2017 (link)). Treated neurons were incubated at 37oC in a 5% CO2 environment for 24 h. Afterward, the cells were harvested, and the cytoplasmic and nuclear fractions were extracted using a NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher). BCA was used to determine protein concentrations and samples were stored in aliquots at −80oC until use.
Ohene-Nyako M., Nass S.R., Richard H.T., Lukande R., Nicol M.R., McRae M., Knapp P.E, & Hauser K.F. (2023). Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia. ASN NEURO, 15, 17590914231158218.
Publication 2023
Antibiotics Brain Cells Cell Survival CX 4945 Cytoplasm Deoxyribonucleases Drugs, Orphan Embryo Glutamate Glutamine Inflammation Mediators Lysine Medulloblastoma Mice, Inbred ICR Morphine Mus Neurons Phosphorylation Poly A Proteins protein TDP-43, human Rare Diseases Ribonucleases silmitasertib Striatum, Corpus Tissue, Membrane Tissues Trypsin
4
Hepatic Fibrosis Induction and Pharmacokinetic Study
Male BALB/c mice were purchased from SLC Inc. (Kotoh-cho, Japan). BALB/c mice were intraperitoneally injected with 100 mg/kg of TAA for 8 weeks, twice a week to induce hepatic fibrosis. The mice were acclimatized for one week prior to experiment and were housed in groups of 3–5 at a temperature of 21 ± 2 °C and 50 ± 5% humidity with a 12 h light/dark cycle.
Seven or eight-week-old ICR mice were purchased from OrientBio Inc. (Siheung, Korea) for the pharmacokinetic study. Mice had free access to food and water. On the experiment day, the mice were fasted for 16 h before oral administration of auranofin or aurocyanide. The animal handlings and experimental procedures were approved by IACUC (Institutional Animal Care and Use Committee, SNU-190924-6 and DGMIF-19071401-00).
Kim H.Y., Otgontenger U., Kim J.W., Lee Y.J., Kim S.B., Lim S.C., Kim Y.M, & Kang K.W. (2023). Anti-fibrotic effect of aurocyanide, the active metabolite of auranofin. Archives of Pharmacal Research, 46(3), 149-159.
Publication 2023
Administration, Oral Animals Auranofin Fibrosis, Liver Food gold cyanide Humidity Institutional Animal Care and Use Committees Males Mice, House Mice, Inbred BALB C Mice, Inbred ICR
5
Transgenic Mouse Models for Neurophysiology
The animal protocols used in the present study were approved by the Animal Care and Use Committee of this institution, in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.
Mice for voltage-sensitive dye imaging came from a colony of CBA/CaJ mice bred in-house (stock #000654; The Jackson Laboratory). CBA mice are commonly used in auditory electrophysiology studies, and their hearing is similar to that of C57 mice (Walton et al., 1995 (link)). When these mice were not available, we purchased outbred ICR mice from Envigo (formerly Harlan Laboratories). Postnatal day (P)16–P22 animals of either sex were used. Auditory brainstem responses were qualitatively similar between strains. Probe expression and imaging signals were qualitatively similar between sexes.
Mice for hVOS imaging and immunohistochemistry staining were bred in the Biotron Laboratory of UW-Madison and our in-house facility. Fos-CreER (TRAP) mice (Guenthner et al., 2013 (link)) were purchased from the Jackson Laboratory (B6.129(Cg)- Fos/J, stock #021882). Our Cre reporter Ai35-hVOS mice (Bayguinov et al., 2017 (link)) are available from the Jackson Laboratory (stock #031102). FosCreER+/− males were mated with hVOS+/+ females to obtain litters with 50% harboring both genes (referred to here as TRAP::hVOS mice). Genotyping was performed after P14 to confirm the presence of the hVOS and Cre genes. No difference in weight, appearance, and behavior was noticed between FosCreER+/− mice and their FosCreER−/− littermates.
Ma Y., Shu W.C., Lin L., Cao X.J., Oertel D., Smith P.H, & Jackson M.B. (2023). Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus. eNeuro, 10(3), ENEURO.0465-22.2023.
Publication 2023
Animals Animals, Laboratory Auditory Brainstem Responses Auditory Perception Electrophysiologic Study, Cardiac Females Gender Genes Males Mice, House Mice, Inbred CBA Mice, Inbred ICR Patient Holding Stretchers Strains

Top products related to «Mice, Inbred ICR»

1
Icr mice by Charles River Laboratories
Sourced in China, Japan, United States, France
ICR mice are a widely used mouse model in biomedical research. They are outbred, multipurpose mice that exhibit genetic variability, making them suitable for a variety of research applications.
2
Icr mice by Japan SLC
Sourced in Japan
ICR mice are a breed of laboratory mice commonly used in scientific research. They are a stock of outbred mice that exhibit genetic diversity, making them a useful model for various studies. The ICR mice are maintained by Japan SLC, a leading provider of high-quality laboratory animals.
3
Icr mice by Orient Bio
Sourced in Cameroon
ICR mice are a common laboratory mouse strain used in scientific research. They are genetically homogenous and serve as a standard model organism. ICR mice exhibit normal growth and development characteristics.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Male icr mice by Charles River Laboratories
Sourced in China, Japan
Male ICR mice are a widely used laboratory mouse strain. They are outbred, albino mice that are characterized by their general vigor and adaptability. These mice are commonly used in a variety of research applications.
6
Male icr mice by Orient Bio
Sourced in Cameroon
Male ICR mice are a commonly used laboratory animal model. They are a type of outbred mouse, which means they have a genetically diverse background. ICR mice are known for their docile temperament and are widely used in a variety of research applications.
7
Icr mice by BioLASCO
Sourced in Taiwan, Province of China
ICR mice are a strain of laboratory mice commonly used in research. They are an outbred mouse strain, meaning they are genetically diverse. ICR mice are known for their high fertility and rapid growth rate.
8
Cd1 icr mice by Charles River Laboratories
Sourced in United States, Japan, China
The CD1 (ICR) mice are a commonly used outbred mouse strain developed and maintained by Charles River Laboratories. They are characterized by their genetic diversity and are often used in various research applications where a heterogeneous population is desired.
9
M2 medium by Merck Group
Sourced in United States, Germany, United Kingdom, France, Japan
M2 medium is a cell culture media formulation designed for the maintenance and cultivation of mouse embryos. It provides the necessary nutrients and growth factors to support the development and growth of mouse embryos in an in vitro environment.
10
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

More about "Mice, Inbred ICR"

Inbred ICR mice, also known as CD1 (ICR) mice or M2 medium mice, are a widely-used strain of albino laboratory mice in biomedical research.
These genetically homogeneous mice have a consistent phenotype, making them a valuable model for studying a variety of diseases and conditions.
Researchers can optimize their Inbred ICR mouse studies by leveraging AI-driven tools like PubCompare.ai, which helps identify the best protocols and products from literature, preprints, and patents.
This enhances reproducibility and accuracy, crucial for reliable research outcomes.
When working with Inbred ICR mice, researchers may also utilize media like FBS (Fetal Bovine Serum) and supplements like Penicillin/Streptomycin to support cell growth and maintain a healthy experimental environment.
By understanding the unique characteristics and research applications of Inbred ICR mice, scientists can design more effective studies and draw meaningful insights from their work.