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Microcebus

Microcebus is a genus of small primates, commonly known as mouse lemurs, native to the island of Madagascar.
These nocturnal, arboreal creatures are the world's smallest primates, weighing only 30-60 grams.
Microcebus species are characterized by their large eyes, pointed snouts, and long, bushy tails.
They play a vital role in the island's ecosystem as pollinators and seed dispersers.
Microcebus research is crucial for understanding the evolution, behavior, and ecology of these fascinating primates, as well as for developing conservation strategies to protect their vulnerable habitats.
Unfortunatley, many Microcebus species are threatened by deforestation and habitat loss.

Most cited protocols related to «Microcebus»

Sequences were downloaded from the National Center for Biotechnology Information (NCBI) SRA (Daubentonia madagascariensis: SRP007603; Pan troglodytes: SRP012268 [SRX142913]). Raw sequences were preprocessed with Prinseq [32 (link)] to remove forward/reverse duplicates and SeqPrep [33 ] to remove adapters and merge overlapping reads. All preprocessed sequences were passed through kmer error correction using BFC [34 (link)] specifying the -s parameter for genome size. Multiplicity distribution of 23mers was carried out with Jellyfish2 [35 (link)] and KrATER [36 ] in order to estimate coverage. De novo genome assembly was performed with SOAPdenovo2 [37 (link)], using the sparse_pregraph module with the following parameters: -g 15 -d 4 -e 4 -R -r 0, and parameter -M 1 during contig phase.
Multiple sets of in silico mate-pairs were generated with Cross-mates. First, paired-end reads of the target organism are mapped onto the reference genome with BWA and default settings [38 ]. Then, a consensus is computed using samtools/bcftools [39 (link)] with the samtools legacy variant calling model. Read pairs are sampled from the consensus in systematic mode, i.e., using exact insert sizes and sampling fragments at regularly spaced offsets, skipping regions of coverage lower than three. For the chimpanzee assembly, 14 scaffolding libraries ranging from 500 bp to 200 kb were generated from the human reference at a 10x coverage. For the aye-aye assembly, 16 scaffolding libraries ranging from 500 bp to 20 kb were generated from the human and lemur references, respectively, at a 10x coverage.
Finally, gaps in the assembly were filled in using SOAPdenovo2 GapCloser [37 (link)]. Assembly statistics and mis-assemblies were measured with Quast [40 (link)]. Completeness and biological accuracy of assembly contiguity were measured by searching for 3,023 vertebrate orthologs as implemented in BUSCO [41 (link)] on a set of protein predictions generated by Augustus 3.1.0 [42 (link)]. Reference assembly sequences used for generating scaffolding libraries and benchmarking were obtained from NCBI: human (GRCh38.p8; GCF_0 00001405); gray mouse lemur Microcebus murinus (Mmur_2.0; GCF_000 165445); aye-aye (DauMad-1.0; GCA_000 241425). All steps used for creating in silico scaffolding libraries, including Cross-mates, have been implemented in the pipeline Cross-Species Scaffolding, which is publicly available and maintained at Github. An example of the Cross-mates command line scripts used for the pork tapeworm assembly experiments is included in Additional file 1.
For the pork tapeworm test assembly, in silico mate pairs were generated using the reference genomes of four species of tapeworms (Taenia saginata, T. asiatica, T. multiceps, and T. solium) at a 10x coverage each, with multiple insert sizes ranging from 600 to 50,000 bp and assembled in SOAPdenovo. For the yeast test, we used a different assembler (SPAdes; [43 ]) for de novo assembly with 10x coverage of 500, 2,000, 5,000, and 10,000 bp insert sizes in silico mate pairs.
Publication 2018
Biopharmaceuticals Cestoda Division Phase, Cell Genome Homo sapiens Lemurs Microcebus Pan troglodytes Saccharomyces cerevisiae saginata, Taenia Staphylococcal Protein A Taenia solium Vertebrates
ERC values were calculated between 17,486 pairs of human genes as described in previous publications [26 (link),28 (link)]. In order to be included in the mammalian ERC analysis, gene ortholog presence was required in a minimum of 17 of the 33 species in the dataset. Of the 19,733 mammalian gene alignments considered, 17,487 met this threshold. Briefly, branch lengths based on amino acid divergence were created from protein coding mammalian sequences derived from the following species: Homo sapiens (human), Pongo pygmaeus abelii (orang-utan), Macaca mulatta (rhesus macaque), Callithrix jacchus (marmoset), Tarsius syrichta (tarsier), Microcebus murinus (mouse lemur), Otolemur garnettii (bushbaby), Tupaia belangeri (tree shrew), Cavia porcellus (guinea pig), Dipodomys ordii (kangaroo rat), Mus musculus (mouse), Rattus norvegicus (rat), Spermophilus tridecemlineatus (squirrel), Oryctolagus cuniculus (rabbit), Ochotona princeps (pika), Vicugna pacos (alpaca), Sorex araneus (shrew), Bos taurus (cow), Tursiops truncatus (dolphin), Pteropus vampyrus (megabat), Myotis lucifugus (microbat), Erinaceus europaeus (hedgehog), Equus caballus (horse), Canis lupus familiaris (dog), Felis catus (cat), Choloepus hoffmanni (sloth), Echinops telfairi (tenrec), Loxodonta africana (elephant), Procavia capensis (rock hyrax), Dasypus novemcinctus (armadillo), Monodelphis domestica (opossum), Macropus eugenii (wallaby), and Ornithorhynchus anatinus (platypus). Branch lengths were estimated using the aaml program of the PAML package [58 (link)]. These lengths were normalized into relative rates using the projection operator method [59 (link)], and correlation coefficients (i.e. ERC values) between these relative rates were calculated between every pair of genes using custom Perl programs.
Publication 2015
Amino Acids Armadillos Bush Babies Callithrix Callithrix jacchus Cavia porcellus Didelphidae Dolphins Echinops telfairi Elephants Equus caballus Erinaceidae Felis catus Genes Homo sapiens Hyraxes Loxodonta Lupus Vulgaris Macaca mulatta Macropus Mammals Mice, House Microcebus Monodelphis domestica Ochotona Open Reading Frames Oryctolagus cuniculus Otolemur Platypus, Duckbilled Pongo abelii Pongo pygmaeus Projective Techniques Pteropus Rabbits Rats, Kangaroo Rattus norvegicus Shrews Sloths Squirrels Susliks Tarsiidae Tarsius Tenrec Tupaia Tupaiidae Tursiops truncatus Vicugna pacos Wallabies Wolves
Our analysis using human as the reference species is based on the human (NCBI36/hg18 assembly) 44-way genome alignment, downloaded from the UCSC genome browser (23 (link)) (http://genome.ucsc.edu/). For the analysis where mouse is the reference species, we extended the mouse (NCBI37/mm9 assembly) 30-way genome alignment provided by the UCSC genome browser by the following assemblies to obtain an alignment with the same species as the human 44-way alignment except lamprey: sloth (choHof1), kangaroo rat (dipOrd1), gorilla (gorGor1), mouse lemur (micMur1), microbat (myoLuc1), pika (ochPri2), rock hyrax (proCap1), megabat (pteVam1), squirrel (speTri1), zebra finch (taeGut1), tarsier (tarSyr1), dolphin (turTru1) and alpaca (vicPac1). We updated the following species using newer assemblies: armadillo (dasNov2), cow (bosTau4), elephant (loxAfr2), guinea pig (cavPor3), horse (equCab2), human (hg19), medaka (oryLat2), opossum (monDom5) and rat (rn5). To build this alignment, we first obtained pairwise genome alignments using lastz (24 (link)) followed by chaining and netting (25 (link)) and then used multiz (26 (link)) with reciprocal-best nets for all species.
To obtain conserved regions, we used PhastCons (2 (link)) most-conserved elements in addition to regions that clearly align to outgroup species, keeping only elements that are ≥70 bp long. Because our focus is on non-coding conserved elements, we excluded all conserved elements that overlap exons contained in the UCSC tracks knownGene, refGene, mgcGenes, ccdsGene, ensGene, exoniphy and vegaGene. To further ensure that these elements are non-coding, we ran Blastx against the non-redundant protein (nr) database and discarded all elements having hits with an E-value of <0.01. We also discarded elements with overlap to snoRNAs, miRNAs, pseudogenes or transposons and portions of elements that are within 100 bp of exon flanks to avoid conserved splicing regulatory regions. As regions with close paralogy elsewhere in the reference genome are prone to mis-alignments of orthologous sequences, we further discarded elements that have a second BLAT hit with a score of ≥40. We always excluded regions on the mitochondrial chromosome as well as random and haplotype chromosomes.
To distinguish subsequent losses of an ancient element from the recent emergence of a conserved element in a clade, we discarded all elements that do not align to at least one of the following outgroup species: opossum (monDom5), platypus (ornAna1), chicken (galGal3), lizard (anoCar1) or zebra finch (taeGut1). The resulting human set comprised 231 653 CNEs totaling 55.7 Mb or 1.8% of the genome. The mouse set comprised 178 775 elements totaling 44.2 Mb or 1.6% of the genome.
To obtain a set of highly conserved elements, we kept only elements for which the fraction of rejected substitutions computed by GERP (27 (link)) is >50% and the conservation P-value from phyloP (28 (link)) is <1020. These strict requirements are fulfilled for the top 37% of the human conserved elements. This set comprised 86 105 elements totaling 28.7 Mb or 0.9% of the human genome.
Publication 2012
Armadillos BP 100 Cavia Chickens Chromosomes Didelphidae DNA, Mitochondrial Dolphins Elephants Equus caballus Exons Finches Genome Genome, Human Gorilla gorilla Haplotypes Homo sapiens Hyraxes Jumping Genes Lampreys Lizards Mice, House Microcebus MicroRNAs Ochotona Oryzias latipes Platypus, Duckbilled Proteins Pseudogenes Rats, Kangaroo Regulatory Sequences, Nucleic Acid Sequence Alignment SLC6A2 protein, human Sloths Small Nucleolar RNA Squirrels Tarsiidae Vicugna pacos Zebras
The 42 male grey mouse lemurs (M. murinus, Cheirogaleidae, primates) used in this study were born in the laboratory breeding colony at Brunoy, France (agreement A91-114-1) from stock originally caught more than 40 years ago on the southwest coast of Madagascar. All animals were included in a single cohort at the age of 38 ± 1 months, which is considered an adult age in this species. The project began at the onset of the winter-like season in the lab. The general conditions of captivity were maintained with respect to ambient temperature (25°C) and relative humidity (55%). The grey mouse lemur is a nocturnal primate that shows high levels of locomotor activity and normothermic body temperature during the night. Just before the onset of the light phase, it enters a torpid state during which it decreases its body temperature and metabolic rate until 4 to 6 h after the beginning of the day when its body temperature returns to normothermic values. The mouse lemur exhibits photoperiod-dependent seasonal variations in most of its physiological functions. Another interesting particularity of this species is its body mass gain during winter. Indeed, at the beginning of winter the body mass of the grey mouse lemur increases by approximately 50% to 70% and only returns to basal levels in the summer. In the breeding colony, animals are exposed to an artificial photoperiodic regimen consisting of six months of summer-like long day length (14:10 h light-darkness, LD) and 6 months of winter-like short day length (10:14 h light-darkness, SD). These photoperiodic regimens are sufficiently discriminating to induce radically different physiological and behavioural responses, as observed in nature in Madagascar. The change of photoperiod occurred abruptly without any significant disturbance for the animals. To minimise social influences during the different experiments, animals were housed individually in 1 m3 cages, provided with a nest and supports and separated from each other by metallic partitions. Animals were weighed once a week to monitor body mass variations. All experiments were performed in accordance with the Principles of Laboratory Animal Care (National Institutes of Health publication 86-23, revised 1985) and French national laws.
Publication 2010
Adult Animals Animals, Laboratory Body Temperature CAGE1 protein, human Cheirogaleidae Childbirth Darkness Human Body Humidity Lethargy Light Locomotion Males Metals Microcebus physiology Primates Treatment Protocols

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Publication 2016
Base Sequence Biological Evolution Callithrix Cavia CD8-Positive T-Lymphocytes Chromatin Immunoprecipitation Sequencing Epigenome Genome Gibbons Gorilla gorilla Histones Homo sapiens Macaca mulatta Mammals Mice, House Microcebus Pan troglodytes Papio hamadryas Pongo pygmaeus Rabbits RNA-Seq T-Lymphocyte Subsets Tarsiidae Transcription Factor Transcriptome Tupaiidae

Most recents protocols related to «Microcebus»

The following animals were used in this study: adult mice 2–12 months old, both male and female; adult treeshrews 7–18 months old, both male and female; adult mouse lemurs 2–3.5 years, both male and female; and adult macaques 3 and 7 years old, male. All procedures on mice, macaques, and treeshrews were conducted in accordance with the ethical guidelines of the National Institutes of Health and were approved by the Institutional Animal Care and Use Committee at the California Institute of Technology.
Mouse lemur experiments were in accordance with European animal welfare regulations and were reviewed by the local ethics committee (Comite d’éthique en expérimentation animale No. 68) in Brunoy, France, by the ethics committee of the University of Geneva, Switzerland and authorized by the French ‘Ministère de l’education nationale de l’enseignement supérieur et de la recherche’.
Publication 2023
Adult Animals Comite Ethics Committees Europeans Females Institutional Animal Care and Use Committees Macaca Males Mice, House Microcebus Regional Ethics Committees Tupaiidae
Four adult mouse lemurs (three male, one female; age: 2–3.5 years) bred and raised in the ‘Mouse Lemur Platform’ (authorization number E-91-114-1) of the ‘Museum National d’Histoire Naturelle’ in Brunoy, France (UMR MECADEV CNRS/MNHN 7179) were trained to perform the figure-ground segregation task. Animals were co-housed 2–3 per cage in a reversed long-day (14:10 light:dark) cycle. They were food restricted, with their body weight maintained above 60 g, but had free access to water. Training was performed during the dark cycle in a custom-made behavioral arena (20 × 20 × 30 cm) containing three optical lickports (Sanworks) situated in a sound-attenuating box. Drops of liquid food rewards (standard food mixture composed of banana, cereal, milk, and egg) was provided upon poking at the appropriate lickports. Images were presented on a Dell P2414H (1920 × 1080, 60 Hz) screen and controlled using Psychopy and Matlab software. Training and testing followed the same paradigm as for treeshrews (Figure 2—figure supplement 2a, b3).
Publication 2023
Adult Animals Banana Body Weight Cereals Dietary Supplements Females Food Males Microcebus Milk, Cow's Sound Tupaiidae Vision
Sucking lice were collected from six Microcebus spp. and two Cheirogaleus spp. during routine live-trapping procedures at ten locations in Madagascar (Table 1, Fig. 1) and stored in individually labelled vials in 70–90% ethanol. At two sites each, two sympatric host species were sampled (M. murinus and M. ravelobensis at Ankarafantsika National Park and C. sibreei and C. crossleyi at Tsinjoarivo), while lice from Microcebus gerpi were collected from three different, geographically separated, populations. Trapping procedures have been described by Durden et al. (2018) (link), Klein et al. (2018) (link) and Schüßler et al. (2020) (link).

Origin and number of sucking lice included in the phylogenetic study.

Table 1
Host speciesSampling locationGPS coordinatesNo. of lice
Microcebus danfossiAnjajavy−15.01 S, 47.24 E3
Microcebus gerpiMandriza Sahafina−18.81 S, 48.98 E5
Microcebus gerpiAndobo−18.90 S, 49.13 E3
Microcebus gerpiAnjahamana−18.39 S, 49.00 E1
Microcebus griseorufusTsimanampetsotsa National Park−24.02 S, 43.74 E10
Microcebus murinusAnkarafantsika National Park−16.32 S, 46.72 E24
Microcebus murinusMariarano−15.48 S, 46.69 E1
Microcebus ravelobensisAnkarafantsika National Park−16.32 S, 46.72 E17
Microcebus rufusRanomafana National Park−21.25 S, 47.42 E3
Cheirogaleus crossleyiAmbatovy−18.87 S, 48.31 E2
Cheirogaleus crossleyiTsinjoarivo−19.69 S, 47.78 E1
Cheirogaleus sibreeiTsinjoarivo−19.69 S, 47.78 E3
Publication 2023
Anoplura Cheirogaleus Ethanol Microcebus Population Group Sympatric Species
Atrial preparations (including the SAN and the right and left atria) were obtained as described previously29 (link). Briefly, after general anesthesia with ketamine and complete loss of hind-limb reflex (see above), we removed the heart from the thoracic cage of the animal. Then, we cut the coronary sinus and, starting from it, we removed the ventricles from the heart. We used a stereomicroscope (SZX16, Olympus; Tokyo, Japan) with low magnification (7×) to trans-illuminate and directly visualize the remaining atrial preparation. We identified the SAN region using the borders of the superior and inferior vena cava, the crista terminalis and the interatrial septum as landmarks77 (link). The atrial preparation was pinned to the bottom of an optical chamber (Fluorodish, FD35PDL-100, WPI; Sarasota, FL) coated with ~ 2 mm of clear Sylgard (Sylgard 184 Silicone elastomer kit; Dow Corning; Midland, MI). To avoid interference from secondary pacemaker tissues we removed the atrioventricular node from the preparation.
For comparative experiments, 24-month-old mice (C57BL/6) were used to match the old age of Microcebus murinus (from 6 to 11 years old) from which we obtain tissue preparation of the SAN. As for Microcebus murinus the investigation with mice conforms to the European directives (2010/63/EU) for the Care and Use of Laboratory Animals and was approved by the French Ministry of Agriculture (N° D34-172-13). Briefly, mice were anaesthetized with 0.01 mg/g xylazine (Rompun 2%, Bayer AG, Leverkusen Germany), 0.1 mg/g ketamine (Imalgène, Merial, Bourgelat France) and 0.2 mg/g Na-Pentobarbital (CEVA, France). Then, after complete loss of hind-limb reflex or any other sensation, we removed the heart from the thoracic cage of the animal and we further dissected it to obtain the entire atrial preparation including the SAN and the atria29 (link).
To analyze voltage changes in the SAN preparation we loaded it by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator Di-4-ANEPPS (2 µmol/L; AAT Bioquest, Sunnyvale, California). This immersion was performed at room temperature (20–22 °C) and lasted for at least 30 min. To maintain proper oxygenation, the chamber containing the tissue was maintained under agitation for the whole loading period. After the loading step, the tissue was washed in dye-free Tyrode’s solution for 15 min. During this step, we slowly increase the temperature to 34–36 °C. The atrial preparation was then constantly superfused at 34–36 °C and imaged by high-speed optical voltage mapping (1000–333 frames/s) on a MiCAM03 Camera—256 × 256 pixel CMOS sensor, 17.6 × 17.6 mm (SciMedia; Costa Mesa, CA). This camera was mounted on a THT microscope, with two objectives (PLANAPO 2X Leica as magnification lens and PLANAPO 1.6X Leica as eyepiece lens) that generated a field of view of 22 × 22 mm. A system constituted by a 150 W halogen light and a built-in shutter (SciMedia; Costa Mesa, California) was used as the excitation source of light for the voltage dye. The filter set included a 531/50 nm excitation filter, 580 nm dichroic mirror, and 580 long-pass emission filter. To avoid motion artefacts, we blocked mechanical activity using blebbistatin (10 µM; Tocris Bioscience; Bristol, UK)29 (link). Optical raw data were analysed using dedicated software from the camera developer, BV workbench (Brainvision; Tokyo, Japan), in combination with ClampFit (ver. 10.0.7, Molecular Devices, LLC; San Jose, California).
Publication 2023
1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine Animals Animals, Laboratory Atrium, Left blebbistatin Cell Respiration Chronic multifocal osteomyelitis Europeans General Anesthesia Halogens Heart Heart Atrium Heart Ventricle Hindlimb Ketamine Lens, Crystalline Light Medical Devices Mice, House Microcebus Microscopy Nodes, Atrioventricular Pacemaker, Artificial Cardiac Pentobarbital Reading Frames Rib Cage Ribs Rompun Septum, Atrial Silicone Elastomers Sinus, Coronary Submersion Tissues Vena Cavas, Inferior Vision Xylazine
When possible, we applied the recommendations stated in the ARRIVE guidelines to the manuscript. According to it, all animal procedures were reviewed and conform with the ethical guidelines for the Care and Use of Laboratory Animals published by the US National Institute of Health (NIH Publication No. 85–23, revised 1996) and European directives (2010/63/EU). Male and female Microcebus murinus, of 1–12 years of age, were used for telemetry recording with minimally invasive or external electrocardiogram devices that allowed reintegration of the animals to their colony after the experimentation. Conversely, only animals from 5 to 12 years, that after natural aging had to be euthanized for factors hampering normal life, like weight loss, were used for all the other experiments. This approach reduces the need of animals in the respect of the “three R’s” of animal welfare: replacement, reduction, and refinement.
Publication 2023
Animals Animals, Laboratory Electrocardiography Europeans Females Males Medical Devices Microcebus Telemetry

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More about "Microcebus"

Microcebus, the genus of small primates commonly known as mouse lemurs, are fascinating creatures native to the island of Madagascar.
These nocturnal, arboreal species are the world's tiniest primates, weighing a mere 30-60 grams.
Characterized by their large eyes, pointed snouts, and bushy tails, Microcebus play a vital role in the island's ecosystem as pollinators and seed dispersers.
Resaerch on these diminutive primates is crucial for understanding their evolution, behavior, and ecology, as well as for developing conservation strategies to protect their vulnerable habitats.
Unfortunately, many Microcebus species face threats from deforestation and habitat loss.
To optimize your Microcebus research, consider utilizing the AI-driven platform of PubCompare.ai.
This solution can help you easily locate protocols from literature, preprints, and patents, and use intelligent comparisons to identify the best protocols and products.
Enhance reproducibility and accuracy in your Microcebus studies with PubCompare.ai's leading platform.
When conducting your Microcebus research, you may also find it useful to explore related technologies and techniques, such as the MiSeq platform, Type 5995 instruments, Rompun anesthesia, SM2400 cameras, ECL detection methods, Anti-mouse immunohistochemistry (PI-2000), NanoZoomer slide scanners, NanoZoomer Digital Pathology System software, Big Dye sequencing chemistry, and PcDNA5/FRT expression vectors.
By leveraging these tools and resources, you can optimize your Microcebus research and gain valuable insights into these fascinating primates.