BSC1 (monkey kidney epithelial) cells stably expressing rat brain clathrin light chain-EGFP (kindly provided by T. Kirchhausen, Harvard Medical School, Boston, MA) were cultured and prepared as specified in Supplementary Note 13 online. For live cell imaging, BSC1 cells were plated on glass coverslips, and through-the-objective TIR-FM was performed on a Nikon TE2000U inverted microscope using a 100X/1.45NA oil-immersion objective. Images were captured at 0.5 Hz with 200ms exposure time using a Hamamatsu Orca II-ERG.
>
Living Beings
>
Mammal
>
Monkeys
Monkeys
Monkeys are a diverse group of primates found in various regions around the world.
They are characterized by their social nature, agility, and distinctive facial features.
Monkeys play a crucial role in medical research, serving as animal models for studying human physiology, behavior, and disease.
Researchers can leverage the power of AI-driven platforms like PubCompare.ai to effortlessly locate the best protocols from literature, pre-prints, and patents, improving the reproducibility and accuracy of monkey-related studies.
This can lead to groundbreaking discoveries and advancements in our understanding of primate biology and its implications for human health.
They are characterized by their social nature, agility, and distinctive facial features.
Monkeys play a crucial role in medical research, serving as animal models for studying human physiology, behavior, and disease.
Researchers can leverage the power of AI-driven platforms like PubCompare.ai to effortlessly locate the best protocols from literature, pre-prints, and patents, improving the reproducibility and accuracy of monkey-related studies.
This can lead to groundbreaking discoveries and advancements in our understanding of primate biology and its implications for human health.
Most cited protocols related to «Monkeys»
Brain
Cells
Clathrin Light Chains
Epithelial Cells
Kidney
Microscopy
Monkeys
Orcinus orca
Submersion
Brain
Cells
Clathrin Light Chains
Epithelial Cells
Kidney
Microscopy
Monkeys
Orcinus orca
Submersion
Human ESC (H9, H1) and iPSC lines (2C6 and SeV6) were subjected to a modified dual SMAD-inhibition13 (link) based FP induction12 (link) protocol. Exposure to SHH C25II, Purmorphamine, FGF8 and CHIR99021 were optimized for midbrain FP and DA neuron yield (see Figure 1d ). Following FP induction, further maturation was carried out in Neurobasal/B27 medium supplemented with AA, BDNF, GDNF, TGFβ3 and dbcAMP (see full methods for details). The resulting DA neurons were subjected to extensive phenotypic characterization via immunocytochemistry, qRT-PCR, gene expression profiling, HPLC analysis for DA and in vitro electrophysiological recordings. In vivo studies were performed in 6-hydroxydopamine lesioned, hemiparkinsonian rodents (adult NOD-SCID IL2Rgc mice and Sprague Dawley rats) as well as in two adult rhesus monkeys treated with carotid injections of MPTP. DA neurons were injected stereotactically in the striata of the animals (150 × 103 cells in mice, 250 × 103 cells in rats) and a total of 7.5 × 106 cells (distributed in 6 tracts; 3 on each side of brain) in monkeys. Behavioral assays were performed at monthly intervals post grafting, including amphetamine mediated rotational analysis as well as a test for focal akinesia (“stepping test”) and forelimb use (cylinder test). Rats and mice were sacrificed at 18–20 weeks and the primates at 1 month post grafting. Characterization of the grafts was performed via stereological analyses of cell numbers and graft volumes and comprehensive immunohistochemistry.
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Adult
Amphetamine
Animals
Biological Assay
Brain
Bucladesine
Carotid Arteries
Cells
Chir 99021
FGF8 protein, human
Forelimb
Glial Cell Line-Derived Neurotrophic Factor
Grafts
High-Performance Liquid Chromatographies
Homo sapiens
Hydroxydopamine
Immunocytochemistry
Immunohistochemistry
Induced Pluripotent Stem Cells
Macaca mulatta
Mesencephalon
Mice, Inbred NOD
Monkeys
Mus
Neurons
Phenotype
Primates
purmorphamine
Rats, Sprague-Dawley
Rattus
Rodent
SCID Mice
Step Test
Striatum, Corpus
Animals
Central Nervous System
Institutional Animal Care and Use Committees
Leeches
Monkeys
Nervousness
Operative Surgical Procedures
Premotor Cortex
Vision
Optical recordings from the isolated leech central nervous system were made by K. Briggman and W. Kristan and have been described previously24 (link),25 (link). We recorded neural activity from trained monkeys using both single- and multi-electrode techniques. We recorded from the arm representation of premotor cortex using a wireless system while the monkey walked to obtain juice from the front of a treadmill. We recorded from the arm representation of motor and premotor cortex while monkeys reached to targets projected onto a vertically oriented screen, also for juice reward. All surgical and animal care procedures were performed in accordance with National Institutes of Health guidelines and were approved by the Stanford University Institutional Animal Care and Use Committee.
Animals
Central Nervous System
Institutional Animal Care and Use Committees
Leeches
Monkeys
Nervousness
Operative Surgical Procedures
Premotor Cortex
Vision
Most recents protocols related to «Monkeys»
Example 3
Reactivity of the antibodies of the invention against several species of mesothelin (cyno, rat, mouse) was tested using assays well known in the art. The data is summarized in
FACS binding assays were performed to evaluate the binding of the anti-Mesothlelin antibodies to murine, rat and cynomologous monkey mesothelin orthologues, using recombinant forms of the various receptors transiently expressed on 293T cells. FACs assays were performed by incubating hybridoma supernatants with 10,000 to 25,000 cells in PBS/2% Fetal bovine serum/2 mM Calcium Chloride at 4° C. for one hour followed by two washes with PBS/2% Fetal bovine serum/2 mM Calcium Chloride. Cells were then treated with florochrome-labeled secondary antibodies at 4° C. followed by one wash. The cells were resuspended in 50 μl of PBS/2% FBS and antibody binding was analyzed using a FACSCalibur™ instrument.
Full text: Click here
Anti-Antibodies
Antibodies
Biological Assay
Calcium chloride
Cells
Cross Reactions
HEK293 Cells
Hybridomas
Immunoglobulins
Mesothelin
Monkeys
Mus
Example 7
Midazolam powder preparations were prepared by blending midazolam spray-dried with MCC and HPMC, and additional MCC. The midazolam powder formulation, midazolam liquid suspension and a midazolam powder formulation in a crystal form will be dosed to monkeys. Plasma midazolam concentrations and the corresponding pharmacokinetic parameters for each preparation will be determined.
Full text: Click here
Midazolam
Monkeys
Plasma
Powder
Example 8
Phenobarbital powder preparations were prepared by blending phenobarbital spray-dried with MCC and HPMC, and additional MCC. The phenobarbital powder formulation, phenobarbital liquid suspension and a phenobarbital powder formulation in a crystal form will be dosed to monkeys. Plasma phenobarbital concentrations and the corresponding pharmacokinetic parameters for each preparation will be determined.
Full text: Click here
Monkeys
Phenobarbital
Plasma
Powder
Example 13
Cynomologous monkeys have sebaceous glands very similar in size to those of human facial skin and at a depth from the skin surface approximating that of the human (˜500 [Figure (not displayed)]m). SCD-1 inhibitors are screened by dosing topically to the upper back of monkeys BID for up to 3 months. Sebum production is analyzed by various methods. SebuTape is used for timed sebum collection and extracted lipids are quantified. Skin biopsies are obtained and changes in sebaceous gland size and surface area are monitored by standard histological evaluation. In both the extracted lipids from SebuTape and the skin biopsies, SCD-1 inhibition is also quantified by measuring inhibition of the conversion of stearoyl-CoA (18:0) to oleoyl-CoA (18:1). Good biological activity in this model is indicated by substantial inhibition of the conversion of stearoyl-CoA (18:0) to oleoyl-CoA (18:1) and substantial reduction in sebaceous gland size and sebum-specific lipids such as wax esters.
Full text: Click here
Biological Models
Biopsy
Esters
Face
Homo sapiens
inhibitors
Lipids
Monkeys
oleoyl-coenzyme A
Psychological Inhibition
Sebaceous Glands
Sebum
Skin
stearoyl-coenzyme A
Example 21
In this example, protease activated CI104, and dually masked CI011, CI090, and CI091 were dosed in cynomolgus monkeys (n=1) at 0.06, 0.18 (activated CI104), or 600 mg/kg (CI011, CI090, CI091). Blood was collected for cytokine analysis pre-dose and at 1 h, 4 h, 8 h, and 24 h post dose. Samples were analyzed using Life Technologies Monkey Magnetic 29-Plex Panel Kit (Product No. LCP0005M). Data was acquired on a BioRad BioPlex 200 instrument. This analysis was conducted in compliance with standard operating procedures at SNBL USA, Ltd. (Everett, WA).
Full text: Click here
Animals
Antibodies
Antibodies, Bispecific
bioplex
BLOOD
Cytokine
Macaca fascicularis
Monkeys
Peptide Hydrolases
T-Lymphocyte
Top products related to «Monkeys»
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in United States, United Kingdom, Germany, Canada, Japan, Sweden, Austria, Morocco, Switzerland, Australia, Belgium, Italy, Netherlands, China, France, Denmark, Norway, Hungary, Malaysia, Israel, Finland, Spain
MATLAB is a high-performance programming language and numerical computing environment used for scientific and engineering calculations, data analysis, and visualization. It provides a comprehensive set of tools for solving complex mathematical and computational problems.
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
Sourced in United States, United Kingdom
Offline Sorter is a software tool for offline analysis of electrophysiology data. It allows users to view, sort, and analyze recorded neural signals.
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
Sourced in Canada
The EyeLink 1000 is a high-performance eye tracker that provides precise and accurate eye movement data. It is capable of recording monocular or binocular eye position at sampling rates up to 2000 Hz. The system uses infrared illumination and video-based eye tracking technology to capture eye movements. The EyeLink 1000 is designed for use in a variety of research applications, including cognitive science, psychology, and human-computer interaction studies.
Sourced in United States, Macao
Purina Monkey Chow is a balanced and nutritious dry food formulated to meet the dietary needs of non-human primates. It provides essential vitamins, minerals, and nutrients required for their overall health and well-being.
Sourced in United States
The High Protein Monkey Diet is a nutritionally-balanced feed formulated to meet the dietary requirements of non-human primates. The product provides a complete and consistent source of protein, carbohydrates, fats, vitamins, and minerals essential for the health and wellbeing of laboratory monkeys.
More about "Monkeys"
Primates, a diverse group of mammals, include the fascinating monkeys found across various regions worldwide.
These social, agile creatures boast distinctive facial features and play a crucial role in medical research as animal models.
Researchers can leverage AI-driven platforms like PubCompare.ai to effortlessly locate the best protocols from literature, preprints, and patents, improving the reproducibility and accuracy of monkey-related studies.
This can lead to groundbreaking discoveries and advancements in our understanding of primate biology and its implications for human health.
Monkeys, a suborder of primates, encompass a wide range of species, from the well-known rhesus macaques to the curious marmosets.
These intelligent animals exhibit complex social behaviors, intricate communication, and impressive physical capabilities.
Researchers utilize monkey models to study human physiology, behavior, and disease, as there are many similarities between primate and human biology.
The use of AI-powered tools, such as PubCompare.ai, empowers scientists to optimize their monkey research.
These platforms enable effortless identification of the best protocols from a vast array of literature, preprints, and patents, ensuring reproducibility and accuracy in their studies.
By leveraging the power of artificial intelligence, researchers can accelerate their discoveries and gain deeper insights into primate biology, ultimately advancing our understanding of human health and well-being.
In the pursuit of groundbreaking discoveries, researchers may also employ specialized equipment and resources, such as FBS (Fetal Bovine Serum), MATLAB (a programming language for numerical computing), DMEM (Dulbecco's Modified Eagle Medium), Penicillin/Streptomycin, Offline Sorter (a neural data analysis tool), EyeLink 1000 (an eye-tracking system), Purina Monkey Chow, and High Protein Monkey Diet.
These tools and materials play a crucial role in supporting various aspects of monkey-related research, from cell culture to behavioral analysis.
By embracing the synergy between cutting-edge technology, like PubCompare.ai, and the wealth of primate research resources, scientists can unlock new frontiers in our understanding of monkeys and their implications for human health.
This interdisciplinary approach, combining AI-driven insights with specialized equipment and diets, holds immense potential for groundbreaking discoveries that will shape the future of medical research and our knowledge of the primate world.
These social, agile creatures boast distinctive facial features and play a crucial role in medical research as animal models.
Researchers can leverage AI-driven platforms like PubCompare.ai to effortlessly locate the best protocols from literature, preprints, and patents, improving the reproducibility and accuracy of monkey-related studies.
This can lead to groundbreaking discoveries and advancements in our understanding of primate biology and its implications for human health.
Monkeys, a suborder of primates, encompass a wide range of species, from the well-known rhesus macaques to the curious marmosets.
These intelligent animals exhibit complex social behaviors, intricate communication, and impressive physical capabilities.
Researchers utilize monkey models to study human physiology, behavior, and disease, as there are many similarities between primate and human biology.
The use of AI-powered tools, such as PubCompare.ai, empowers scientists to optimize their monkey research.
These platforms enable effortless identification of the best protocols from a vast array of literature, preprints, and patents, ensuring reproducibility and accuracy in their studies.
By leveraging the power of artificial intelligence, researchers can accelerate their discoveries and gain deeper insights into primate biology, ultimately advancing our understanding of human health and well-being.
In the pursuit of groundbreaking discoveries, researchers may also employ specialized equipment and resources, such as FBS (Fetal Bovine Serum), MATLAB (a programming language for numerical computing), DMEM (Dulbecco's Modified Eagle Medium), Penicillin/Streptomycin, Offline Sorter (a neural data analysis tool), EyeLink 1000 (an eye-tracking system), Purina Monkey Chow, and High Protein Monkey Diet.
These tools and materials play a crucial role in supporting various aspects of monkey-related research, from cell culture to behavioral analysis.
By embracing the synergy between cutting-edge technology, like PubCompare.ai, and the wealth of primate research resources, scientists can unlock new frontiers in our understanding of monkeys and their implications for human health.
This interdisciplinary approach, combining AI-driven insights with specialized equipment and diets, holds immense potential for groundbreaking discoveries that will shape the future of medical research and our knowledge of the primate world.