In vivo cell-attached recordings were performed using glass pipettes (∼5-7 MΩ) filled with solution containing the following (in mM): 125 NaCl, 5 KCl, 10 glucose, 10 HEPES, 2 CaCl2, 2 MgSO4, and 0.1 Alexa Fluor 594; pH 7.4). Signals were amplified using an AxoPatch 200B amplifier (Molecular Devices), filtered at 5 kHz, and digitized at 10 kHz. Spikes were recorded using current clamp mode. The frame trigger pulses of ScanImage 4.0 were also recorded and used offline to synchronize individual frames to electrophysiological recordings. After establishment of a low-resistance seal (15-50 MΩ), the orientation, spatial and temporal frequency of the stimuli was quickly optimized for individual neurons using recorded spikes. The optimal grating stimulus was repeated at a reduced contrast to maintain a moderate spiking rate.
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Phocidae
Phocidae
Phocidae, also known as earless seals or true seals, are a family of semiaquatic marine mammals that belong to the order Pinnipedia.
These seals are characterized by their lack of external ears, their streamlined bodies, and their ability to propel themselves through the water using their hind flippers.
Phocidae are found in cold and temperate regions around the world, including the Arctic and Antarctic Oceans, as well as in coastal areas of North America, Europe, and Asia.
They play an important role in marine ecosystems, serving as predators and prey, and are the subject of ongoing research and conservation efforts.
Phocidae include well-known species such as the harbor seal, the ringed seal, and the elephant seal.
Undestand the power of PubCompare.ai to streamline your Phocidae reseach today.
These seals are characterized by their lack of external ears, their streamlined bodies, and their ability to propel themselves through the water using their hind flippers.
Phocidae are found in cold and temperate regions around the world, including the Arctic and Antarctic Oceans, as well as in coastal areas of North America, Europe, and Asia.
They play an important role in marine ecosystems, serving as predators and prey, and are the subject of ongoing research and conservation efforts.
Phocidae include well-known species such as the harbor seal, the ringed seal, and the elephant seal.
Undestand the power of PubCompare.ai to streamline your Phocidae reseach today.
Most cited protocols related to «Phocidae»
Alexa594
Cells
Glucose
HEPES
Medical Devices
Neurons
Phocidae
Precipitating Factors
Pulses
Reading Frames
Sodium Chloride
Sulfate, Magnesium
In vivo cell-attached recordings were performed using glass pipettes (∼5-7 MΩ) filled with solution containing the following (in mM): 125 NaCl, 5 KCl, 10 glucose, 10 HEPES, 2 CaCl2, 2 MgSO4, and 0.1 Alexa Fluor 594; pH 7.4). Signals were amplified using an AxoPatch 200B amplifier (Molecular Devices), filtered at 5 kHz, and digitized at 10 kHz. Spikes were recorded using current clamp mode. The frame trigger pulses of ScanImage 4.0 were also recorded and used offline to synchronize individual frames to electrophysiological recordings. After establishment of a low-resistance seal (15-50 MΩ), the orientation, spatial and temporal frequency of the stimuli was quickly optimized for individual neurons using recorded spikes. The optimal grating stimulus was repeated at a reduced contrast to maintain a moderate spiking rate.
Alexa594
Cells
Glucose
HEPES
Medical Devices
Neurons
Phocidae
Precipitating Factors
Pulses
Reading Frames
Sodium Chloride
Sulfate, Magnesium
PCR verified expression constructs were transformed into either B834 (DE3) or Rosetta(DE3)LysS E. coli (Novagen) in 96-tube format as described for OmniMaxII transformations with 1% w/v Glucose replacing the X-Gal and IPTG reagents in the LB agar. All plates and subsequent media used for the culture of the Rosetta(DE3) LysS cells are identical to those described for B834(DE3) cells but also supplemented with 35 µg/ml chloramphenicol to maintain the pRareLysS plasmid. Plates were incubated for 18 h at 37°C before individual colonies were used to inoculate, 500 µl GS96 (Bio101, QBioGene, Cambridge, UK) supplemented with 0.05% v/v glycerol, 1% w/v glucose and 50 µg/ml carbenicillin in 96-well deep-well plates. The plates were sealed with gas-permeable adhesive seals and shaken at 225 r.p.m. at 37°C for 18 h. For IPTG induction of expression, 50 µl of each overnight culture was then used to inoculate (in four 24-well deep-well plates) 2.5 ml of GS96 supplemented with 50 µg/ml carbenicillin. The diluted cultures were grown at 37°C with shaking at 225 r.p.m., for 3 h before reducing the temperature to 20°C, addition of ITPG to a final concentration of 0.5 mM and shaking for a further 18 h at 20°C. For auto-induction of expression, 50 µl of each overnight culture was used to inoculate (in four 24-well deep-well plates) 2.5 ml of Overnight Express™ Instant TB media (Novagen) supplemented with 50 µg/ml carbenicillin. The diluted cultures were grown at 37°C with shaking at 225 r.p.m., for 3 h before reducing the temperature to 25°C and shaking for a further 24 h at 25°C.
A 1.5 ml aliquot of culture from each well was then transferred to a 2 ml 96-well deep-well plate using a Theonyx robot (Aviso Gmbh, Gera, Germany) and harvested by centrifugation at 6000 g for 10 min at 4°C before decanting of the waste media. Pelleted cells were frozen at −80°C for at least 30 min prior to screening for soluble His6-tag protein expression using either the Theonyx or BR8000 robotic platforms with standard Qiagen Ni-NTA magnetic bead protocols (as per manufacturer's instructions). Proteins purified by elution from the Ni-NTA beads were analysed on SDS-PAGE gels (Criterion™ 10–20% gradient gels—Biorad, Hemel Hempstead, UK or InVitrogen NuPAGE™ Novex 10% Bis-Tris Midi gels with MES buffer system) and visualized with SafeStain™ (InVitrogen). Scale-up and purification of proteins from E. coli were carried out as described earlier (11 (link)).
A 1.5 ml aliquot of culture from each well was then transferred to a 2 ml 96-well deep-well plate using a Theonyx robot (Aviso Gmbh, Gera, Germany) and harvested by centrifugation at 6000 g for 10 min at 4°C before decanting of the waste media. Pelleted cells were frozen at −80°C for at least 30 min prior to screening for soluble His6-tag protein expression using either the Theonyx or BR8000 robotic platforms with standard Qiagen Ni-NTA magnetic bead protocols (as per manufacturer's instructions). Proteins purified by elution from the Ni-NTA beads were analysed on SDS-PAGE gels (Criterion™ 10–20% gradient gels—Biorad, Hemel Hempstead, UK or InVitrogen NuPAGE™ Novex 10% Bis-Tris Midi gels with MES buffer system) and visualized with SafeStain™ (InVitrogen). Scale-up and purification of proteins from E. coli were carried out as described earlier (11 (link)).
5-bromo-4-chloro-3-indolyl beta-galactoside
Agar
Altretamine
Bistris
Buffers
Carbenicillin
Cells
Centrifugation
Chloramphenicol
Escherichia coli
Freezing
Gels
Glucose
Glycerin
his6 tag
Isopropyl Thiogalactoside
Lysine
Permeability
Phocidae
Plasmids
Proteins
SDS-PAGE
The ddPCR workflow was described in Figure 1 . The TaqMan PCR reaction mixture was assembled from a 2× ddPCR Mastermix (Bio-Rad), 20× primer, and probes (final concentrations of 900 and 250 nM, respectively) and template (variable volume) in a final volume of 20 μL. Each assembled ddPCR reaction mixture was then loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 60 μL of droplet generation oil (Bio-Rad) was loaded into the oil well for each channel. The cartridge was placed into the droplet generator (Bio-Rad). The cartridge was removed from the droplet generator, where the droplets that collected in the droplet well were then manually transferred with a multichannel pipet to a 96-well PCR plate. The plate was heat-sealed with a foil seal and then placed on a conventional thermal cycler and amplified to the end-point (40–55 cycles). After PCR, the 96-well PCR plate was loaded on the droplet reader (Bio-Rad), which automatically reads the droplets from each well of the plate (32 wells/h). Analysis of the ddPCR data was performed with QuantaSoft analysis software (Bio-Rad) that accompanied the droplet reader.
Oligonucleotide Primers
Phocidae
For spot size measurements, fresh heparinized blood was spotted onto sheets of Whatman no 1, Whatman 3 MM (Whatman, Maidstone, UK) or glass fibre (Printed Filtermat A, Perkin Elmer, Beaconsfield, UK) filter paper in volumes from 1 μl to 45 μl. For antibody stability and recovery studies, reconstituted blood was prepared by mixing individual African malaria-hyperimmune plasma samples or a hyperimmune plasma pool or a European (non-exposed) negative control pool (all containing heparin) 1:1 by volume with washed European blood group O erythrocytes and immediately spotting 10 μl of this mixture onto Whatman 3 MM or glass fibre filter paper. Spots were allowed to dry at ambient temperature and relative humidity (RH) overnight.
Laboratory-generated blood spots and blood spots from Uganda were stored in individual self-sealing plastic bags (25 cm × 25 cm approx), which were then combined into sets and stored within three further successive plastic bags, the innermost of which contained approximately 3 g of self-indicating silica desiccant gel type III (Sigma). The bags were inspected regularly to confirm that the desiccant remained blue (RH < 20%), and the desiccant replaced if necessary.
Fingerprick blood samples from lower Moshi were collected into EDTA-coated microtainers and as blood spots on Whatman 3 MM paper and transported daily to the laboratory at Kilimanjaro Christian Medical College. Filter papers were refrigerated (2–8°C) in individual plastic bags with silica gel. Plasma was separated from packed red blood cells after centrifugation and stored at -20°C. All samples were assayed within 8 weeks of collection.
Laboratory-generated blood spots and blood spots from Uganda were stored in individual self-sealing plastic bags (25 cm × 25 cm approx), which were then combined into sets and stored within three further successive plastic bags, the innermost of which contained approximately 3 g of self-indicating silica desiccant gel type III (Sigma). The bags were inspected regularly to confirm that the desiccant remained blue (RH < 20%), and the desiccant replaced if necessary.
Fingerprick blood samples from lower Moshi were collected into EDTA-coated microtainers and as blood spots on Whatman 3 MM paper and transported daily to the laboratory at Kilimanjaro Christian Medical College. Filter papers were refrigerated (2–8°C) in individual plastic bags with silica gel. Plasma was separated from packed red blood cells after centrifugation and stored at -20°C. All samples were assayed within 8 weeks of collection.
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BLOOD
Centrifugation
Desiccants
Edetic Acid
Erythrocytes
Europeans
Exanthema
Heparin
Humidity
Immunoglobulins
Malaria
Negroid Races
Phocidae
Plasma
Silica Gel
Most recents protocols related to «Phocidae»
Example 2
A carbonic acid triggerable polymer was made with 38.2% Methyl methacrylate (MMA), 60.3% 2-(dimethylamino)ethyl methacrylate (DMAEMA), and 1.5% ethylene glycol dimethacrylate (EGDMA). Collapsed, these particles were opaque-white, and turned semi-translucent when swollen.
Although embodiments described herein are made with reference to example embodiments, it should be appreciated by those skilled in the art that various modifications are well within the scope of this disclosure. Those skilled in the art will appreciate that the example embodiments described herein are not limited to any specifically discussed application and that the embodiments described herein are illustrative and not restrictive. From the description of the example embodiments, equivalents of the elements shown therein will suggest themselves to those skilled in the art, and ways of constructing other embodiments using the present disclosure will suggest themselves to practitioners of the art. Therefore, the scope of the example embodiments is not limited herein.
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2-(dimethylamino)ethyl methacrylate
Carbonic Acid
Dental Cements
ethylene dimethacrylate
ethylmethacrylate
Methylmethacrylate
Phocidae
Polymers
Example 6
Yeast colonies verified to contain the expected UGT gene were picked into 96-well microtiter plates containing Bird Seed Media (BSM, originally described by van Hoek et al., Biotechnology and Bioengineering 68(5), 2000, pp. 517-523) with 20 g/L sucrose and 37.5 g/L ammonium sulfate. Cells were cultured at 30° C. in a high capacity microtiter plate incubator shaking at 1000 rpm and 80% humidity for 3 days until the cultures reached carbon exhaustion. The growth-saturated cultures were subcultured into fresh plates containing BSM with 40 g/L sucrose and 150 g/L ammonium sulfate by taking 14.4 μl from the saturated cultures and diluting into 360 μl of fresh media. Cells in the production media were cultured at 30° C. in a high capacity microtiter plate shaker at 1000 rpm and 80% humidity for an additional 3 days prior to extraction and analysis. Upon completion the whole cell broth is diluted with 360 uL of 100% ethanol, sealed with a foil seal, and shaken at 1250 rpm for 30 min to extract the steviol glycosides. 490 uL of 50:50 ethanol:water is added to a new 1.1 mL assay plate and 10 uL of the culture/ethanol mixture is added to the assay plate. The mixture is centrifuged to pellet any solids, and 400 uL of the solution is transferred to a new 1.1 mL plate and assayed by LC-MS.
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Aves
Carbon
Cells
Ethanol
Genes
Humidity
Phocidae
stevioside
Sucrose
Sulfate, Ammonium
Yeast, Dried
Example 88
A mixture of 3-[chloro(difluoro)methyl]-6-[5-fluoro-6-[rac-(1R)-2,2,2-trifluoro-1-methyl-ethoxy]-3-pyridyl]-[1,2,4]triazolo[4,3-a]pyrazine (500 mg, 1.21 mmol) and AgBF4 (2.36 g, 12.15 mmol) in Methanol (5 mL) was stirred at 90° C. for 6 hours in a seal tube. After cooling to 25° C., the mixture was quenched with brine (20 mL), diluted with EtOAc (20 mL) and filtered through Celite. The filtrate was extracted with EtOAc (20 mL×3), and the combined organic phase was washed with brine (20 mL), dried over Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (EtOAc in PE=0% to 10% to 30%) to give the product (290 mg) as an oil. The impure product was purified by prep-HPLC (Xtimate C18 (150 mm×25 mm, 5 μm) A=H2O (0.075% NH4OH) and B=CH3CN; 51-81% B over 11 minutes) to give the product (256.79 mg, 0.63 mmol) as a solid. 1H NMR (400 MHz CDCl3) δH=9.52 (d, 1H), 8.50 (d, 1H), 8.45 (d, 1H), 8.05 (dd, 1H), 5.95-5.85 (m, 1H), 3.98 (s, 3H), 1.60 (d, 3H). LCMS Rt=1.33 min in 2 min chromatography, 10-80AB, MS ESI calcd. for C15H12F6N5O2 [M+H]+ 407.9. found 407.9.
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1H NMR
brine
Celite
Chromatography
High-Performance Liquid Chromatographies
Lincomycin
Methanol
Phocidae
Pyrazines
Silica Gel
Example 12
In another embodiment of Method 2, the second coloring liquid is not mixed with the adhesive solution. First, the zirconia base after surface roughening treatment is colored with the second coloring liquid. When the color of the zirconia base is observed to be consistent with that of the patient's teeth, coloring is stopped, the zirconia base is oven-dried and then soaked in the adhesive solution for 1-20 min, so that the adhesive solution seals the coloring liquid in the pores of the zirconia and fills the pores on the surface of the zirconia to form a protective film on or over the surface of the zirconia ceramic, which prevents bacteria, enzymes and other substances in the oral cavity from entering the pores and causing infection and other hazards. Finally, the zirconia base is taken out, put into a denture sintering furnace, heated up to 1530° C. at a rate of 5° C./min, kept at this temperature for crystallization for 120 min, and then cooled down along with the furnace.
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Bacteria
Crystallization
Dentures
Enzymes
Infection
Oral Cavity
Phocidae
Tooth
zirconium oxide
EXAMPLE 2
-
- 1) Dissolve Gelatin and other ingredients in purified water under stirring at 200-500 rpm.
- 2) Make up the final volume of the solution using purified water.
- 3) Mix the solution under stirring at 200 to 500 rpm for further 15 min.
- 4) Dose the solution into each cavity of preformed blister sheets (preferably using dispensing pipette).
- 5) Freeze the filled blisters at a temperature in the range of −20 to −110° C.
- 6) Freeze dry the blisters in a lyophilizer.
- 7) Place the blister sheet containing dried lyophilisates on the punched carrier web of the blister packaging machine to transport the blister sheets through the sealing station of the packaging machine
- 8) Seal the blister with a lidding foil and punch into final blisters.
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Aspartame
Autism Spectrum Disorders
Dental Caries
Freezing
Gelatins
Mannitol
methylparaben, sodium salt
Phocidae
propylparaben
Sodium
Ticagrelor
Zinc
Top products related to «Phocidae»
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The Axopatch 200B is a high-performance patch-clamp amplifier designed for electrophysiology research. It is capable of amplifying and filtering electrical signals from single-cell preparations, providing researchers with a tool to study ion channel and membrane properties.
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Sylgard 184 is a two-part silicone elastomer system. It is composed of a siloxane polymer and a curing agent. When mixed, the components crosslink to form a flexible, transparent, and durable silicone rubber. The core function of Sylgard 184 is to provide a versatile material for a wide range of applications, including molding, encapsulation, and coating.
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The Multiclamp 700B amplifier is a versatile instrument designed for electrophysiology research. It provides high-quality amplification and signal conditioning for a wide range of intracellular and extracellular recording applications. The Multiclamp 700B offers advanced features and precise control over signal acquisition, enabling researchers to obtain reliable and accurate data from their experiments.
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The Digidata 1440A is a high-performance data acquisition system designed for a variety of electrophysiology applications. It features 16-bit analog-to-digital conversion, multiple sampling rates, and simultaneous acquisition of multiple channels. The Digidata 1440A provides the hardware interface for recording and digitizing electrophysiological signals.
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PClamp 9 software is a comprehensive data acquisition and analysis software designed for electrophysiology research. It provides a suite of tools for recording, visualizing, and analyzing electrical signals from cells and tissues.
More about "Phocidae"
Explore the fascinating world of Phocidae, the family of semiaquatic marine mammals also known as earless seals or true seals.
These remarkable creatures, part of the Pinnipedia order, are characterized by their lack of external ears, streamlined bodies, and impressive ability to propel themselves through the water using their powerful hind flippers.
Phocidae can be found in cold and temperate regions around the globe, from the Arctic and Antarctic Oceans to the coastal areas of North America, Europe, and Asia.
They play a vital role in marine ecosystems, serving as both predators and prey, and are the subject of ongoing research and conservation efforts.
Discover the diverse species that make up the Phocidae family, including the well-known harbor seal, ringed seal, and the majestic elephant seal.
Learn about their unique adaptations and behaviors, and explore the latest scientific insights into their biology, ecology, and the crucial role they play in maintaining the delicate balance of marine environments.
Enhance your Phocidae research with the powerful tools and capabilities of PubCompare.ai, an AI-driven platform that revolutionizes the way you approach your studies.
Easily locate relevant protocols from literature, pre-prints, and patents, and leverage AI-driven comparisons to identify the best protocols and products for your research.
Streamline your workflow and stay ahead of the curve with PubCompare.ai's intuitive interface and cutting-edge technology.
Whether you're a scientist, researcher, or simply fascinated by these captivating marine mammals, PubCompare.ai can help you take your Phocidae exploration to new heights.
Experience the future of Phocidae research today and unlock the full potential of your studies.
Explore the insights and resources available through PubCompare.ai and embark on a journey of discovery in the enigmatic world of Phocidae.
These remarkable creatures, part of the Pinnipedia order, are characterized by their lack of external ears, streamlined bodies, and impressive ability to propel themselves through the water using their powerful hind flippers.
Phocidae can be found in cold and temperate regions around the globe, from the Arctic and Antarctic Oceans to the coastal areas of North America, Europe, and Asia.
They play a vital role in marine ecosystems, serving as both predators and prey, and are the subject of ongoing research and conservation efforts.
Discover the diverse species that make up the Phocidae family, including the well-known harbor seal, ringed seal, and the majestic elephant seal.
Learn about their unique adaptations and behaviors, and explore the latest scientific insights into their biology, ecology, and the crucial role they play in maintaining the delicate balance of marine environments.
Enhance your Phocidae research with the powerful tools and capabilities of PubCompare.ai, an AI-driven platform that revolutionizes the way you approach your studies.
Easily locate relevant protocols from literature, pre-prints, and patents, and leverage AI-driven comparisons to identify the best protocols and products for your research.
Streamline your workflow and stay ahead of the curve with PubCompare.ai's intuitive interface and cutting-edge technology.
Whether you're a scientist, researcher, or simply fascinated by these captivating marine mammals, PubCompare.ai can help you take your Phocidae exploration to new heights.
Experience the future of Phocidae research today and unlock the full potential of your studies.
Explore the insights and resources available through PubCompare.ai and embark on a journey of discovery in the enigmatic world of Phocidae.