> Living Beings > Plant > Aloe

← Alnus

Aloe

Q: What are the different types or varieties of Aloe?

Aloe is a large genus of succulent plants that includes over 500 species. Some of the most well-known varieties include Aloe vera, Aloe barbadensis, Aloe arborescens, and Aloe ferox. Each type has slightly different properties and uses, ranging from cosmetic applications to medicinal purposes.

Aloe, a succulent plant genus known for its medicinal properties, has a rich history of use in traditional and modern healthcare.
This AI-driven platform, PubCompare.ai, helps researchers uncover the power of Aloe by providing access to the best protocols, products, and insights from scientific literature, preprints, and patents.
Explore data comparisons to optimize your Aloe research and ensure reproducibility, paving the way for the future of Aloe-based therapies.
Diceover the potential of this versatile plant with PubCompare.ai today!

Most cited protocols related to «Aloe»

Taxonomic study of Aspergillus section Fumigati

The fungi examined included type strains or representatives of all species
available for examination in Aspergillus section Fumigati.
Some atypical isolates collected in Australia and Papua New-Guinea were also
examined to clarify their taxonomic status
(Table 1).

Table 1.

Aspergillus section Fumigati isolates used in this
study.

Species	Isolate No.*	Source
A. brevipes	CBS 118.53^T	Soil, Australia
A. duricaulis	CBS 481.65^T	Soil, Buenos Aires, Argentina
A. fumigatiaffinis	IBT12703^T	Soil, U.S.A.
A. fumigatus	CBS 133.61^T = NRRL 163	Chicken lung, U.S.A.
A. fumisynnematus	IFM 42277^T	Soil, Venezuela
A. lentulus	CBS 117887^T = NRRL 35552 = KACC 41940	Man, U.S.A.
A. novofumigatus	IBT 16806^T	Soil, Ecuador
A. unilateralis	CBS 126.56^T	Rhizosphere, Australia
A. viridinutans	CBS 127.56^T	Rabbit dung, Australia
A. turcosus	KACC 42090 = IBT 27920	Air conditioner, Inchen, Korea
	KACC 42091^T = IBT 27921	Air conditioner, Seoul, Korea
	KACC 41955 = CBS 117265= IBT 3016	Car air conditioner, Seoul, Korea
N. assulata	KACC 41691^T	Tomato soil, Buyeo, Korea
N. aurata	CBS 466.65^T	Jungle soil, Brunei
N. aureola	CBS 105.55^T	Soil, Tafo, Ghana
N. australensis sp. nov	CBS 112.55^T = NRRL 2392 = IBT 3021	Garden soil, Adelaide, Australia
N. coreana	KACC 41659^T = NRRL 35590 = CBS 121594	Tomato soil, Buyeo, Korea
N. denticulata	CBS 652.73^T = KACC 41183	Soil under Elaeis guineensis, Suriname
	CBS 290.74 = KACC 41175	Acer pseudoplatanus, Netherlands
N. fennelliae	CBS 598.74^T	Eye ball of Oryctolagus cuniculus, U.S.A.
	CBS 599.74	Eye ball of Oryctolagus cuniculus, U.S.A.
N. ferencziisp. nov.	CBS 121594^T = IBT 27813 = NRRL 4179	Soil, Australia
N. fischeri	CBS 544.65^T = NRRL 181	Canned apples
N. galapagensis	CBS 117522^T = IBT 16756 = KACC 41935	Soil, Ecuador
	CBS 117521 = IBT 16763 = KACC 41936	Soil, Ecuador
N. glabra	CBS 111.55^T	Rubber scrab from old tire, Iowa, U.S.A.
N. hiratsukae	CBS 294.93^T	Aloe juice, Tokyo, Japan
N. laciniosa	KACC 41657^T = NRRL 35589 = CBS 117721	Tomato soil, Buyeo, Korea
N. multiplicata	CBS 646.95^T = IBT 17517	Soil, Mouli, Taiwan
N. nishimurae	IFM 54133 = IBT 29024	Forest soil, Kenya
N. nishimurae	CBS 116047	Cardboard, Netherlands
N. papuensissp. nov.	CBS 841.96^T = IBT 27801	Bark of Podocarpus sp. (Podocarpaceae), bark, Myola, Owen Stanley Range, Northern Province, Papua New Guinea
N. pseudofischeri	NRRL 20748^T = CBS 208.92	Human vertebrate, U.S.A.
N. quadricincta	CBS 135.52^T = NRRL 2154	Cardboard, York, U.K.
	CBS 107078	Soil, Korea
	CBS 100942	Fruit juice, Netherlands
	CBS 253.94	Canned oolong tea beverage, Japan (type strain of N. primulina)
N. spathulata	CBS 408.89^T	Soil under Alocasia macrorrhiza, Taiwan
N. spinosa	CBS 483.65^T	Soil, Nicaragua
N. stramenia	CBS 498.65^T	Soil from maple-ash-elm forest, Wisconsin, U.S.A.
N. tatenoi	CBS 407.93^T	Soil of sugarcane, Timbauba, Brazil
	CBS 101754	Fruit, Yunnan, China (type strain of N. delicata)
N. udagawae	CBS 114217^T	Soil, Brazil
	CBS 114218	Soil, Brazil
N. warcupiisp. nov.	NRRL 35723^T	Arid soil, Finder”s Range, Australia

CBS = Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands; IBT =
Institute for Biotechnology, Lyngby, Technical University of Denmark; IFM =
Institute for Food Microbiology (at present, the Research Center for
Pathogenic Fungi and Microbial Toxicoses, Chiba University), Chiba, Japan;
KACC = Korean Agricultural Culture Collection, Suwon, Korea; NRRL =
Agricultural Research Service Culture Collection, Peoria, Illinois, U.S.A.; T
= type strain.

Samson R.A., Hong S., Peterson S.W., Frisvad J.C, & Varga J. (2007). Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya. Studies in Mycology, 59, 147-203.

Publication 2007

Acer Adrenal Cortex Alocasia macrorrhiza Aloe Aspergillus Beverages Chickens Feces Food Microbiology Forests Fruit Fruit Juices Fungi Homo sapiens Koreans Lung Lycopersicon esculentum Oryctolagus cuniculus Pathogenicity Rabbits Rhizosphere Rubber Saccharum Strains Vertebrates

Optimizing RNA Extraction from Recalcitrant Plant Tissues

Four young tissues, buds, leaves, cambium scrapings and roots, were collected as described previously.[1 (link)] Flowers at full bloom, fruits at flower fall, five-centimeters-high seedlings and young shoot segments without bark below the buds were also collected. The other recalcitrant plant tissues used in this study are listed in Table 2. After collection, all plant materials were immediately frozen in liquid nitrogen and stored at −80 °C until needed.

Table 1.

Quality of RNA extracted from seven tissues.

	NanoDrop 1000^a			2100 Bioanalyzer^b
Plant tissue	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀	ng/μL	RIN^c	rRNA ratio (28S/18S)
Bud	2.14 ± 0.04	2.12 ± 0.01	1014 ± 167	9.4	2.2
Leaf	2.16 ± 0.02	2.21 ± 0.21	2238 ± 430	8.7	1.5
Cambium region	2.13 ± 0.01	2.12 ± 0.12	1119 ± 214	9.5	1.9
Root	2.14 ± 0.10	2.13 ± 0.14	945 ± 231	8.9	2.2
Shoot segment	2.14 ± 0.03	2.17 ± 0.09	723 ± 35	9.6	2.0
Flower	2.14 ± 0.01	2.06 ± 0.23	260 ± 7	9.2	2.4
Fruit	2.19 ± 0.00	2.25 ± 0.05	1044 ± 100	9.1	2.4
Seedling	2.17 ± 0.01	2.16 ± 0.17	1331 ± 146	9.1	2.7

^aResults represent the means ± standard deviation of three samples.

^bThe results of one biological replicate are shown.

^cRIN – RNA integrity number.

Table 2.

Purity and yield of total RNA extracted from recalcitrant plant tissues.

Plant species	Tissues	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀	Concentration (ng/μL)
Camellia sinensis L. (tea) [31 ]	Bud	2.12	1.98	3032
	Young leaf	2.2	2.05	3519
	Fully expanded leaf	2.18	1.93	736

Eriobotrya japonica Lindl. (loquat) [32 (link)]	Terminal bud	2.19	1.95	670
	Young leaf	2.16	2.04	735
	Adult leaf	2.15	2.18	637

Pinus taeda L. (loblolly pine) [33 ]	Young needle	1.86	1.94	296
	Adult needle	2.01	1.90	273

Litchi chinensis Sonn. (lychee) [34 (link)]	Young leaf	2.19	2.05	1924*
	Adult leaf	2.18	2.18	1180

Rosa chinensis (rose) [32 (link)]	Young petal	1.82	1.97	445
	Adult petal	1.80	1.95	388

Taxus media (taxus) [35 (link)]	Young leaf	2.13	2.14	3150*
	Adult leaf	2.04	2.18	2873

Ginkgo biloba L. (ginkgo) [35 (link)]	Young leaf	2.01	1.93	447
	Adult leaf	2.16	2.12	460

Opuntia ficus-indica L. (cactus) [36 ]	Cladode	2.05	1.95	255*

Aloe barbadensis Mill. (curacao aloe) [36 ]	Leaf	2.18	2.21	277*

Note: *The yield given by the simple method was higher than that in the corresponding reference described by p < 0.05.

Ouyang K., Li J., Huang H., Que Q., Li P, & Chen X. (2014). A simple method for RNA isolation from various tissues of the tree Neolamarckia cadamba. Biotechnology, Biotechnological Equipment, 28(6), 1008-1013.

Publication 2014

Adult Aloe Aloe vera Biopharmaceuticals Cactaceae Cambium Camellia sinenses DNA Replication Eriobotrya Freezing Fruit Ginkgo biloba Kidney Cortex Litchi chinensis Loquats Nitrogen Opuntia ficus-indica Pinus Pinus taeda Plant Roots Plants Seedlings Taxus Tissues Young Adult

Phylogenomic Analysis of Xanthorrhoeaceae

A dataset was assembled representing seven plastid and nuclear DNA regions in 239 taxa in Xanthorrhoeaceae, including 197 species in the genera Aloe, Aloidendron, Aloiampelos, Aristaloe, Gonialoe and Kumara. We generated 480 new sequences from leaf or floral specimens collected from natural populations or from curated living collections and DNA banks held primarily at the Royal Botanic Gardens, Kew. A further 279 sequences were obtained from GenBank (ncbi.nlm.nih.gov/genbank/), including 93 rbcL and 64 psbA sequences. Agapanthus africanus (Amaryllidaceae) was used as the outgroup taxon in all analyses.
Total genomic DNA was isolated from fresh plant material (ca. 1 g) or specimens dried in silica gel (ca. 0.3 g) using a modified CTAB protocol [19 ] or the Qiagen DNeasy kit (Qiagen, Copenhagen). Sequences of ITS, matK and trnL-F were amplified using methodology previously described by [6 (link)]. The trnQ-rps16 region was amplified with the primers trnQ(UUG)Aloe (5′-ATCTTRATACAATGTGATCCAC-3′; this study) and rps16x1 [20 (link)]. Sequences from the complementary strands were obtained for all taxa whenever possible, using the BigDye Terminator v3.1 on a 3730 DNA Analyzer (Applied Biosystems/Hitachi). Sequences were assembled in Sequencher 4.8 (Gene Codes, Ann Arbor) and submitted to GenBank (Additional file 1). Sequences were aligned automatically using MUSCLE [21 (link)] implemented with default settings in SeaView v4.2.12 [22 (link)], and adjusted manually in BioEdit v7.1.11 [23 ]. The DNA regions were aligned separately before the data were concatenated using an R [24 ] script to produce a final dataset comprising 240 taxa and 6732 nucleotides in seven DNA regions.
We used Bayesian inference, maximum likelihood and parsimony to produce a phylogenetic hypothesis for Aloe and allied genera, using single-partition (ITS, matK, rps16, psbA, rbcL, trnL-F intron and spacer) and combined datasets. We ran all analyses on the Cyber Infrastructure for Phylogenetic Research (CIPRES) portal [25 ]. Separate parsimony analyses of the ITS (175 taxa, 799 nucleotides) and plastid (231 taxa, 5933 nucleotides) datasets were undertaken with the parsimony ratchet implemented in PAUPRat [26 (link)], to check for strongly supported phylogenetic conflicts (bootstrap percentages >75), before proceeding with analyses based on a total evidence approach using all characters. A maximum likelihood analysis, comprising 1000 bootstrap replicates followed by a heuristic tree search, was executed in RAxML [27 ] with each partition assigned specific parameters under the recommended GTRCAT model. An additional 530 gaps and indels in the combined dataset of all DNA regions were coded using the algorithm described by [28 (link)] in the FastGap v1.2 interface [29 ]. Finally, we ran a Bayesian analysis of the combined dataset with gaps coded in MrBayes v3.1.2 [30 (link)]. Best-fitting models for each data partition for Bayesian inference were identified using the Akaike Information Criterion calculated in Modeltest v3.8 [31 (link)]. The Hasegawa, Kishino and Yano (HKY) model with gamma-shaped distribution of rate heterogeneity among sites (HKY + G) was selected for the ITS, matK, trnQ-rps16 and trnL-F data partitions, while the General Time Reversible (GTR) model with gamma distribution of rate heterogeneity among sites was selected for psbA (GTR + G), and with a proportion of invariable sites (GTR + I + G) for rbcL. For the Bayesian analysis, the parameters were unlinked between loci and four Metropolis Coupled Markov Chains with heating increments of 0.2 were run for 50 million generations and sampled every 1000th generation. The resulting parameters were summarised in Tracer 1.5.0 [32 ]. A quarter of the least likely trees were discarded, and a majority rule consensus tree with branch supports expressed as posterior probabilities (PP) was produced from the remaining trees.

Grace O.M., Buerki S., Symonds M.R., Forest F., van Wyk A.E., Smith G.F., Klopper R.R., Bjorå C.S., Neale S., Demissew S., Simmonds M.S, & Rønsted N. (2015). Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera. BMC Evolutionary Biology, 15, 29.

Publication 2015

Aloe Amaryllidaceae ARID1A protein, human Cetrimonium Bromide Character DNA Library Gamma Rays Genetic Code Genetic Heterogeneity Genome INDEL Mutation Introns MATK protein, human Muscle Tissue Nucleotides Oligonucleotide Primers Plant Leaves Plants Plastids Population Group Silica Gel Trees Xanthorrhoeaceae

Antimicrobial Activity Assessment of Aloe Samples

For qualitative determination of antimicrobial activity of aloe samples, a Kirby–Bauer disk diffusion method [48 (link)] was used. A total of 100 µL of microorganism solution was smeared on the agar plates. Then, 9 mm sterile cellulose discs were put on the inoculated agar plate. Further, 50 µL of potential antimicrobial sample was applied on the disc. Additionally, a control or a “blank” was applied, as 50 µL of physiological solution was placed on sterile cellulose disc. The incubation of the inoculated plates took place under optimal conditions, i.e., at the optimal temperature (Table 4) for each individual microorganism for 24 h. After the incubation period, the diameter of the resulting inhibition zone was measured, which was a scale for antimicrobial efficiency of the samples. All qualitative tests were performed in triplicates, and all associated standard deviations are shown separately in each subchapter.

Kupnik K., Primožič M., Knez Ž, & Leitgeb M. (2021). Antimicrobial Efficiency of Aloe arborescens and Aloe barbadensis Natural and Commercial Products. Plants, 10(1), 92.

Publication 2021

Agar Aloe Cellulose Kirby-Bauer Disk-Diffusion Method Microbicides physiology Psychological Inhibition Sterility, Reproductive

Diverse Botanical Extracts: Preparation and Characterization

Raw materials (with abbreviations) selected for the preparation of extracts used in this study included:

Alv L–aloe leaves, Aloe vera (L.) Burm. f.;

Am Fr–black chokeberry fruits, Aronia melanocarpa (Michx.) Elliott;

Arv H–common mugwort herb, Artemisia vulgaris L.;

Bv R–beetroot roots, Beta vulgaris L.;

Co F–common marigold flowers, Calendula officinalis L.;

Ea H–field horsetail herb, Equisetum arvense L.;

Ep F–purple coneflower flowers, Echinacea purpurea (L.) Moench;

Ep L–purple coneflower leaves, Echinacea purpurea (L.) Moench;

Hp H–St. John’s wort herb, Hypericum perforatum L.;

Hr Fr–sea-buckthorn fruits, Hippophae rhamnoides L.;

Lc S–red lentil seeds, Lens culinaris Medik.;

Mc F–chamomile flowers, Matricaria chamomilla L.;

Ob H–basil herb, Ocimum basilicum L.;

Pm H–broadleaf plantain herb, Plantago major L.;

Poa H–common knotgrass herb, Polygonum aviculare L.;

Ps S–pea seeds, Pisum sativum L.;

Pta L–common bracken leaves, Pteridium aquilinum (L.) Kuhn;

Sg L–giant goldenrod leaves, Solidago gigantea Ait.;

So R–comfrey roots, Symphytum officinale L.;

To F–common dandelion flowers, Taraxacum officinale (L.) Weber ex F.H. Wigg.;

To L–common dandelion leaves, Taraxacum officinale (L.) Weber ex F.H. Wigg.;

To R–common dandelion roots, Taraxacum officinale (L.) Weber ex F.H. Wigg.;

Tp F–red clover flowers, Trifolium pratense L.;

Ur L–nettle leaves, Urtica dioica L.;

Ur R–nettle roots, Urtica dioica L.;

Vo R–valerian roots, Valeriana officinalis L.

Godlewska K., Pacyga P., Szumny A., Szymczycha-Madeja A., Wełna M, & Michalak I. (2022). Methods for Rapid Screening of Biologically Active Compounds Present in Plant-Based Extracts. Molecules, 27(20), 7094.

Publication 2022

Aloe Aloe vera Artemisia Artemisia vulgaris Beta vulgaris Calendula officinalis Chamomile Comfrey Echinacea Echinacea purpurea Equisetum Flowers Fruit Gigantism Hippophae rhamnoides horsetail herb Hypericum perforatum Lens culinaris Lentils Matricaria chamomilla Ocimum basilicum Photinia melanocarpa Pisum sativum Plantago Plant Embryos Plant Roots Polygonum Pteridium esculentum Solidago Taraxacum officinale Trifolium pratense Urtica dioica Valeriana extract Valeriana officinalis

Most recents protocols related to «Aloe»

Cytotoxicity of Plant Stem Cell Extracts

Not available on PMC !

Example 7

The MTT Cell Proliferation assay determines cell survival following apple stem cell extract treatment. The purpose was to evaluate the potential anti-tumor activity of apple stem cell extracts as well as to evaluate the dose-dependent cell cytotoxicity.

Principle: Treated cells are exposed to 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). MTT enters living cells and passes into the mitochondria where it is reduced by mitochondrial succinate dehydrogenase to an insoluble, colored (dark purple) formazan product. The cells are then solubilized with DMSO and the released, solubilized formazan is measured spectrophotometrically. The MTT assay measures cell viability based on the generation of reducing equivalents. Reduction of MTT only occurs in metabolically active cells, so the level of activity is a measure of the viability of the cells. The percentage cell viability is calculated against untreated cells.

Method: A549 and NCI-H520 lung cancer cell lines and L132 lung epithelial cell line were used to determine the plant stem cell treatment tumor-specific cytotoxicity. The cell lines were maintained in Minimal Essential Media supplemented with 10% FBS, penicillin (100 U/ml) and streptomycin (100 μg/ml) in a 5% CO₂at 37 Celsius. Cells were seeded at 5×10³cells/well in 96-well plates and incubated for 48 hours. Triplicates of eight concentrations of the apple stem cell extract were added to the media and cells were incubated for 24 hours. This was followed by removal of media and subsequent washing with the phosphate saline solution. Cell proliferation was measured using the MTT Cell Proliferation Kit I (Boehringer Mannheim, Indianapolis, IN) New medium containing 50 μl of MTT solution (5 mg/ml) was added to each well and cultures were incubated a further 4 hours. Following this incubation, DMSO was added and the cell viability was determined by the absorbance at 570 nm by a microplate reader.

In order to determine the effectiveness of apple stem cell extracts as an anti-tumor biological agent, an MTT assay was carried out and IC50 values were calculated. IC50 is the half maximal inhibitory function concentration of a drug or compound required to inhibit a biological process. The measured process is cell death.

Results: ASC-Treated Human Lung Adenocarcinoma Cell Line A549.

TABLE 7

Results of cytotoxicity of apple stem cell extract on lung cancer cell

line A549 as measured by MTT assay (performed in triplicate).

Values of replicates are % of cell death.

Concentration*replicatereplicatereplicateMean of% Live

(μg/ml)123replicatesSDSEMCells

25093.1890.8690.3491.461.510.878.54

10086.8885.1885.6985.920.870.5014.08

5080.5879.4981.0480.370.800.4619.63

2574.2873.8176.3974.831.380.7925.17

12.567.9868.1371.7569.282.131.2330.72

6.2561.6762.4567.1063.742.931.6936.26

3.12555.3756.7762.4558.203.752.1641.80

1.56249.0751.0857.8052.654.572.6447.35

0.78142.7745.4053.1547.115.403.1252.89

Results: ASC-Treated Human Squamous Carcinoma Cell Line NCI-H520.

TABLE 8

Results of cytotoxicity of apple stem cell extract on lung cancer

cell line NCI-H520 measured by MTT assay (performed in triplicate).

Values of replicates are % of cell death.

Concen-%

tration*replicatereplicatereplicateMean ofLive

(μg/ml)123replicatesSDSEMcell

25088.2889.2987.7388.430.790.4611.57

10078.1379.1978.1378.480.610.3521.52

5067.9869.0968.5468.540.560.3231.46

2557.8358.9958.9458.590.660.3841.41

12.547.6848.8949.3448.640.860.5051.36

6.2537.5338.7939.7538.691.110.6461.31

3.12527.3728.6930.1528.741.390.8071.26

1.56217.2218.5920.5618.791.680.9781.21

0.781 7.07 8.4810.96 8.841.971.1491.16

Results: ASC-treated Lung Epithelial Cell Line L132.

TABLE 9

Results of cytotoxicity of apple stem cell extract on

lung epithelial cell line L132 as measured by MTT assay

(performed in triplicate). Values of replicates are % of cell death.

Concen-rep-rep-rep-Mean%

tration*licatelicatelicateofLive

(μg/ml)123replicatesSDSEMcell

25039.5142.5244.0342.022.301.3357.98

10032.9334.4433.6933.690.750.4466.31

5030.6028.9430.5230.020.940.5469.98

2527.9627.8127.1327.630.440.2572.37

12.525.6225.5525.4025.520.120.0774.48

6.2523.1320.8718.6120.872.261.3179.13

3.12513.3411.0811.8312.081.150.6687.92

1.562 6.56 7.31 9.57 7.811.570.9192.19

0.781 8.06 4.30 3.54 5.302.421.4094.70

Summary Results: Cytotoxicity of Apple Stem Cell Extracts.

TABLE 10

IC50 values of the apple stem cell extracts on the on the target

cell lines as determined by MTT assay.

Target Cell

LineIC50

A54912.58

NCI-H52010.21

L132127.46

Apple stem cell extracts killed lung cancer cells lines A549 and NCI-H520 at relatively low doses: IC50s were 12.58 and 10.21 μg/ml respectively as compared to 127.46 μg/ml for the lung epithelial cell line L132. Near complete anti-tumor activity was seen at a dose of 250 μg/ml in both the lung cancer cell lines. This same dose spared more than one half of the L132 cells. See Tables 7-10. The data revealed that apple stem cell extract is cytotoxic to lung cancer cells while sparing lung epithelial cells. FIG. 6 shows a graphical representation of cytotoxicity activity of apple stem cell extracts on lung tumor cell lines A549, NCIH520 and on L132 lung epithelial cell line (marked “Normal”). The γ-axis is the mean % of cells killed by the indicated treatment compared to unexposed cells. The difference in cytotoxicity levels was statistically significant at p≤05.

Example 9

The experiment of Example 7 was repeated substituting other plant materials for ASC. Plant stem cell materials included Dandelion Root Extract (DRE), Aloe Vera Juice (AVJ), Apple Fiber Powder (AFP), Ginkgo Leaf Extract (GLE), Lingonberry Stem Cells (LSC), Orchid Stem Cells (OSC) as described in Examples 1 and 2. The concentrations of plant materials used were nominally 250, 100, 50, 25, 6.25, 3.125, 1.562, and 0.781 μg/mL. These materials were tested only for cells the human lung epithelial cell line L132 (as a proxy for normal epithelial cells) and for cells of the human lung adenocarcinoma cell line A549 (as a proxy for lung cancer cells).

A549 cells lung cancer cell line cytotoxicity results for each of the treatment materials.

DRE-Treated Lung Cancer Cell Line A549 Cells.

TABLE 11

Triplicate results of cell death of DRE-treated

A549 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration%

(μg/mL)-DRE-Live

treated A549% of cell deathMeanSDSEMcell

25080.4376.4074.8477.232.891.6722.77

10067.6075.2663.7768.885.853.3831.12

5065.3262.9459.9462.732.701.5637.27

2556.8357.9748.1454.315.383.1145.69

6.2555.5949.6949.1751.483.572.0648.52

3.12551.7648.4545.3448.523.211.8551.48

1.56243.6944.0036.0241.244.522.6158.76

0.78137.4726.1919.5727.749.055.2372.26

AVJ-Treated Lung Cancer Cell line A549 Cells.

TABLE 12

Triplicate results of cell death of AVJ-treated

A549 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration%

(μg/mL)-AVJ-treatedLive

A549% of cell deathMeanSDSEMcell

25076.8178.1675.8876.951.140.6623.05

10076.4075.2673.7175.121.350.7824.88

5065.3266.1559.9463.803.371.9536.20

2550.1048.4556.6351.734.322.5048.27

6.2547.5246.3846.1746.690.720.4253.31

3.12539.8638.6143.7940.752.701.5659.25

1.56232.4019.7730.5427.576.823.9472.43

0.78120.5015.6332.1922.778.514.9277.23

AFP-Treated Lung Cancer Cell line A549 Cells.

TABLE 13

Triplicate results of cell death of AFP-treated

A549 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration%

(μg/mL)-AFP-treatedLive

A549% of cell deathMeanSDSEMcell

25086.1387.9986.6586.920.960.5613.08

10079.5081.0682.0980.881.300.7519.12

5073.6072.4671.3372.461.140.6627.54

2568.0167.7066.9867.560.530.3132.44

6.2560.8762.1160.7761.250.750.4338.75

3.12549.4851.7650.7250.661.140.6649.34

1.56240.0641.7247.0042.933.622.0957.07

0.78139.2337.7836.8537.961.200.6962.04

GLE-treated Lung Cancer Cell line A549 Cells.

TABLE 14

Triplicate results of cell death of GLE-treated

A549 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration%

(μg/mL)-GLE-treatedLive

A549% of cell deathMeanSDSEMcell

25088.4291.4990.4490.121.560.909.88

10084.3983.7783.1683.770.610.3516.23

5079.4781.5876.7579.272.421.4020.73

2573.6072.5471.4072.511.100.6327.49

6.2562.8963.6859.9162.161.991.1537.84

3.12550.1854.4751.8452.162.171.2547.84

1.56246.9344.3043.3344.851.861.0755.15

0.78139.5639.3940.9639.970.870.5060.03

LSC-treated lung cancer cell lines A549 cells.

TABLE 15

Triplicate results of cell death of LSC-treated

A549 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration

(μg/mL)% Live

LSC treated A549% of cell deathMeanSDSEMcell

25077.5478.8578.2078.200.650.3821.80

10077.1476.0476.5976.590.550.3223.41

5066.4268.5266.8267.251.120.6532.75

2559.8067.2264.1663.733.732.1536.27

6.2550.5348.8248.0749.141.260.7350.86

3.12541.1443.6042.7242.491.240.7257.51

1.56239.4739.7440.6139.940.600.3460.06

0.78138.5531.8336.7935.723.482.0164.28

OSC-treated Lung Cancer Cell line A549 Cells.

TABLE 16

Triplicate results of cell death of OSC-treated

A549 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration

(μg/mL)% Live

OSC-treated A549% of cell deathMeanSDSEMcell

25070.8465.5771.4969.303.251.8730.70

10048.8150.9157.2852.334.412.5547.67

5046.5949.6053.3349.843.381.9550.16

2538.7740.8136.5838.722.111.2261.28

6.2535.7440.7941.0539.193.001.7360.81

3.12534.5533.6837.0235.081.731.0064.92

1.56233.8633.4427.6331.643.482.0168.36

0.78121.3220.0034.8225.388.214.7474.62

L132 cells (“normal” lung epithelial cell line) cytotoxicity results for each of the treatment materials.

DRE-Treated Lung Epithelial Cell Line L132 cells.

TABLE 17

Triplicate results of cell death of DRE-treated

L132 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates.

Concentration% of %

(μg/mL)cellLive

DRE-treated L132deathMeanSDSEMcell

25086.6686.6186.6686.640.030.0213.36

10076.2977.3976.8476.840.550.3223.16

5065.9268.1767.0167.031.130.6532.97

2555.5458.9557.1957.231.700.9842.77

6.2545.1749.7347.3747.422.281.3252.58

3.12534.8040.5037.5437.612.851.6562.39

1.56224.4231.2827.7227.813.431.9872.19

0.78114.0522.0617.8918.004.012.3182.00

AVJ-Treated Lung Epithelial Cell Line L132 cells.

TABLE 18

Triplicate results of cell death of AVJ-treated

L132 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates

AFP-treated lung epithelial cell line L132 cells.

Concentration % of %

(μg/mL)cellLive

AVJ-treated L132deathMeanSDSEMcell

25057.0355.9353.6255.531.741.0044.47

10050.9949.7847.0449.272.031.1750.73

5044.9543.6340.4543.012.311.3456.99

2538.9137.4933.8636.752.601.5063.25

6.2532.8831.3427.2830.502.891.6769.50

3.12526.8425.1920.6924.243.181.8475.76

1.56220.8019.0514.1117.983.472.0082.02

0.78114.7612.90 7.5211.733.762.1788.27

AFP-Treated Lung Epithelial Cell Line L132 cells.

TABLE 19

Triplicate results of cell death of AFP-treated

L132 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates

AFP-treated lung epithelial cell line L132 cells.

Concentration

(μg/mL)% Live

AFP-treated L132% of cell deathMeanSDSEMcell

25056.1555.4357.1956.260.880.5143.74

10049.9548.2447.6448.611.200.6951.39

5043.7441.0538.0940.962.831.6359.04

2537.5433.8628.5433.324.532.6166.68

6.2531.3426.6718.9925.676.243.6074.33

3.12525.1419.489.4418.027.954.5981.98

1.56218.9412.2910.8714.034.312.4985.97

0.78112.73 5.10 6.81 8.214.002.3191.79

GLE-Treated Lung Epithelial Cell Line L132 cells.

TABLE 20

Triplicate results of cell death of GLE-treated

L132 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates

AFP-treated lung epithelial cell line L132 cells.

Concentration

(μg/mL)% Live

GLE-treated L132% of cell deathMeanSDSEMcell

25084.4283.2083.0883.570.740.4316.43

10080.0579.2978.5979.310.730.4220.69

5072.7571.5974.1072.811.260.7227.19

2580.0581.8679.9980.631.060.6119.37

6.2568.2670.1368.2668.881.080.6231.12

3.12560.6263.0760.6261.441.410.8238.56

1.56248.0748.7748.8348.560.420.2451.44

0.78146.2745.5746.6746.170.560.3253.83

LSC-Treated Lung Epithelial Cell Line L132 cells.

TABLE 21

Triplicate results of cell death of LSC-treated

L132 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates

AFP-treated lung epithelial cell line L132 cells.

Concentration

(μg/mL)% Live

LSC-treated L132% of cell deathMeanSDSEMcell

25086.4185.8285.7686.000.350.2014.00

10081.2181.2779.9980.820.720.4219.18

5075.9674.7473.5174.741.230.7125.26

2574.7472.7571.4772.991.650.9527.01

6.2570.1368.3268.2668.901.060.6131.10

3.12554.0358.0553.4455.172.511.4544.83

1.56253.9751.9851.9852.641.150.6647.36

0.78146.7945.6244.9245.78 0.940.54 54.22

OSC-Treated Lung Epithelial Cell Line L132 cells.

TABLE 22

Triplicate results of cell death of OSC-treated

L132 cells measured by MTT assay.

Percentage of live cells calculated as 100% − Mean of triplicates

AFP-treated lung epithelial cell line L132 cells.

Concentration %

(μg/mL)Live

OSC-treated L132% of cell deathMeanSDSEMcell

25061.8462.3760.4461.551.000.5738.45

10054.1453.4452.1053.231.040.6046.77

5042.9442.3040.3241.851.370.7958.15

2535.9434.4833.3134.581.320.7665.42

6.2533.9632.6732.0332.890.980.5767.11

3.12527.4826.2026.7226.800.650.3773.20

1.562 9.80 7.29 7.35 8.151.430.8391.85

0.781 7.29 8.98 8.05 8.110.850.4991.89

Calculated values.

TABLE 23

Calculated IC50 doses (ug/mL) and therapeutic ratios

(IC50 for L132 cells/IC50 for A549 cells) for each

treatment material. Values greater than one indicate

that a material would be more selective in killing cancer

cells than normal cells. ASC results imported from

Example 8. These studies indicate that at least

some of the materials may be effective anti-cancer agents.

ASC has outstanding selectivity compared to other materials.

ASCDREAVJAFPGLELSCOSC

A549 12.589.82211.4811.9811.1 13.733.9

IC50

L132 127.4656.88 62.6682.6577.6369.26715.38

IC50

Ther.10.15.8 5.56.97.0 0.70.5

Ratio

US11872258B2. Oral cavity treatments (2024-01-16). None [US]. Inventors: Maryam Rahimi [US].

Patent 2024

14-3-3 Proteins 43-63 61-26 A549 Cells Action Potentials Adenocarcinoma of Lung Aloe Aloe vera Antineoplastic Agents Biological Assay Biological Factors Biological Processes Bromides Cardiac Arrest Cell Death Cell Extracts Cell Lines Cell Proliferation Cells Cell Survival Cytotoxin diphenyl DNA Replication Epistropheus Epithelial Cells Fibrosis Formazans Genetic Selection Ginkgo biloba Ginkgo biloba extract Homo sapiens Lingonberry Lung Lung Cancer Lung Neoplasms Malignant Neoplasms Mitochondria Mitochondrial Inheritance Neoplasms Neoplastic Stem Cells Oral Cavity PEG SD-01 Penicillins Pharmaceutical Preparations Phosphates Plant Cells Plant Leaves Plant Roots Plants Powder Psychological Inhibition Saline Solution SD 31 SD 62 SEM-76 Squamous Cell Carcinoma Stem, Plant Stem Cells Streptomycin Succinate Dehydrogenase Sulfoxide, Dimethyl Taraxacum Tetrazolium Salts

Traditional Aloe Leaf Extraction Protocol

A. arboscenes Mill was selected based on its history of use in South African traditional medicine. Fresh leaves of the aloe were obtained in March 2021 at the University of Zululand, KwaDlangezwa campus, South Africa (latitude 28.753° S, longitude 31.894° E, altitude 117 m). Its voucher specimen number (MN01) was deposited in the University of Zululand Herbarium [ZULU]. Ethical approval to collect the plant was acquired from the research ethical committee at the University of Zululand (UZREC 171110-030 PGM 2021/56). The whole leaves of the aloe were washed with tap water to remove soil and debris, air-dried in the fume hood and ground to powder.

Maliehe T.S., Nqotheni M.I., Shandu J.S., Selepe T.N., Masoko P, & Pooe O.J. (2023). Chemical Profile, Antioxidant and Antibacterial Activities, Mechanisms of Action of the Leaf Extract of Aloe arborescens Mill. Plants, 12(4), 869.

Publication 2023

Aloe Medicine, African Traditional Plants Powder

Extraction of Aloe Phytochemicals

Hundred millilitres of a mixture of ethanol (70%) and methanol (80%) at a ratio of 1:10 was added to extract the phytochemicals from the ground aloe powder (10 mg). After 3 days of extraction, the extract was filtered using Whatman No.1 filter paper and subsequently concentrated by evaporating the solvents under fume hood. The extract was re-constituted in acetone and made to the final concentration of 10 mg/mL [52 ].

Publication 2023

Acetone Aloe Ethanol Methanol Phytochemicals Powder Solvents Strains

Aloe-based Alginate Wafer Fabrication

An aloe solution (1 L of 0.1 mol/L) was produced by dissolving 100 mL of pure aloe in 900 mL of deionised water. Sodium alginate powder (2.4 g) was dissolved in 240 mL 0.1 mol/L aloe solution for 24 h to produce a 1% w/v alginate: liquid aloe solution. Sodium alginate powder (12 g) was dissolved in 240 mL 0.1 mol/L aloe solution for 24 h to produce a 5% w/v alginate: liquid aloe solution. Sodium alginate powder (24 g) was dissolved in 240 mL 0.1 mol/L aloe solution for 24 h to produce a 10% w/v alginate: liquid aloe solution.
To each of the 240 mL alginate aloe solutions, 2.4 mL of 0.1 mol/L silver nitrate was added to create an alginate aloe silver solution which was stirred at room temperature under standard conditions for a total 24 h. A 30 mL aliquot of solution was extracted from the alginate aloe silver solution at the time points of 1 hr, 3 h, 5 h, 7 h, 9 h, 11 h and 24 h. From each of these 30 mL solution samples, 3 mL was ejected onto 10 separate mini petri dishes. These were then placed in a LEC Medical Freezer LSFSF39UK for 48 h before they were then moved to the Christ Alpha 1-2 LD plus freeze dryer for a period of 72 h under the conditions of 0.3 bar and −52 °C. After freeze drying, all of the samples were then submerged in a 10 mL 0.1 mol/L calcium chloride solution for 0.5 h before a triplicate wash with deionised water. This, in turn, created polymer wafer disks that were 2 mm, 3 mm and 4 mm in thickness with respect to 1%, 5% and 10% w/v alginate.

Man E., Easdon C., McLellan I., Yiu H.H, & Hoskins C. (2023). Exploration of Nanosilver Calcium Alginate-Based Multifunctional Polymer Wafers for Wound Healing. Pharmaceutics, 15(2), 483.

Publication 2023

Alginate Aloe Calcium chloride Freezing Hartnup Disease Hyperostosis, Diffuse Idiopathic Skeletal Polymers Powder Silver Silver Nitrate Sodium Alginate

Polymer Purification and Characterization

NA polymer samples were fully dissolved in 40 mL deionised water within a 50 mL Corning centrifuge tube. The samples were then centrifuged in a Thermo Scientific Heraeus Multifuge X1R centrifuge (Waltham, MA, USA) for 5 min at 14,500 rpm. The supernatant was discarded, and the centrifuge tube was then filled with 10 mL absolute ethanol, thereby submerging the pellet for 10 min. The ethanol was then discarded without disturbing the pellet. The pellet was then then gently dislodged from the centrifuge tube and transferred into a glass vial where it then air dried for 1 h.
5 w/v and 10% w/v NAA polymer samples were fully dissolved in 40 mL deionised water within a 50 mL Corning centrifuge tube. The samples were then centrifuged in a Thermo Scientific Heraeus Multifuge X1R centrifuge for 20 min at 14,500 rpm. The supernatant was discarded without disturbing the hydrogel pellet and another 40 mL deionised water was added to the centrifuge before undergoing centrifugation using the same parameters. The supernatant was discarded, and the centrifuge tube was then filled with 10 mL absolute ethanol, submerging the pellet for 10 min. The ethanol was discarded, and the pellet was then transported into a glass vial where it was air dried for 1 h before analysis.
Triplicate 1% w/v NAA polymer samples were fully dissolved in 40 mL deionised water within a 50 mL Corning centrifuge tube. The samples were then centrifuged in a Thermo Scientific Heraeus Multifuge X1R centrifuge for 0.5 h at 14,500 rpm. The supernatant was discarded without disturbing the hydrogel pellet, where 10 mL absolute ethanol was then added into the tube and left for 5 min. Excess aloe gel was then suspended within the absolute ethanol, which was then discarded. An additional 10 mL absolute ethanol was added into the tube, submerging the pellet, and was left for 20 min. The ethanol was discarded, and the pellet was then transported into a glass vial where it is air dried for 1 h before analysis.
These samples were then analysed on a Quanta FEG Scanning electron microscope under high vacuum via secondary electron detector mode, with a spot size of 3 at 30 kV.

Man E., Easdon C., McLellan I., Yiu H.H, & Hoskins C. (2023). Exploration of Nanosilver Calcium Alginate-Based Multifunctional Polymer Wafers for Wound Healing. Pharmaceutics, 15(2), 483.

Publication 2023

1-naphthylarsonic acid Aloe Centrifugation Electrons Ethanol Hydrogels Polymers Scanning Electron Microscopy Vacuum

Top products related to «Aloe»

Gallic acid by Merck Group

Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia

Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.

Analytical grade methanol by Sangon

Sourced in China

Analytical-grade methanol is a high-purity solvent used in various laboratory applications. It serves as a critical component in analytical techniques, providing a reliable and consistent medium for chemical reactions, sample preparation, and instrumental analysis.

Nahco3 by Sangon

Sourced in China

NaHCO3 is a chemical compound that is commonly used as a laboratory reagent. It is a white crystalline solid that is soluble in water and has a slightly salty taste. NaHCO3 is the chemical formula for sodium bicarbonate.

Phosphoric acid by Sangon

Sourced in China

Phosphoric acid is a colorless, odorless, and highly corrosive inorganic acid. It is a chemical compound with the formula H3PO4. Phosphoric acid is commonly used as a laboratory reagent and in various industrial applications.

Floid cell imaging station by Thermo Fisher Scientific

Sourced in United States, United Kingdom, India, Australia, Canada

The FLoid Cell Imaging Station is a compact and easy-to-use fluorescence microscope designed for cell imaging. It allows users to capture high-quality images and videos of cells, tissues, and other biological samples.

Cd 1 mice by Charles River Laboratories

Sourced in United States, China, Canada, United Kingdom, Germany, Italy, France, Japan, Spain

CD-1 mice are a widely used outbred mouse strain that exhibit genetic diversity. They are suitable for a variety of research applications due to their adaptability and lack of specific genetic modifications.

2 2 diphenyl 1 picrylhydrazyl (dpph) by Merck Group

Sourced in United States, Germany, Italy, India, China, Spain, Poland, France, United Kingdom, Australia, Brazil, Singapore, Switzerland, Hungary, Mexico, Japan, Denmark, Sao Tome and Principe, Chile, Malaysia, Argentina, Belgium, Cameroon, Canada, Ireland, Portugal, Israel, Romania, Czechia, Macao, Indonesia

DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.

Methanol by Merck Group

Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan

Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

No 1 filter paper by Cytiva

Sourced in United Kingdom, Germany, United States, Japan, Switzerland, China, Italy, India

Cytiva No. 1 filter paper is a high-quality laboratory filtration product designed for general-purpose filtration tasks. It is composed of cellulose fibers and is suitable for a variety of applications requiring efficient separation of solids from liquids.

E2695 by Waters Corporation

Sourced in United States, United Kingdom, Germany, China

The E2695 is a high-performance liquid chromatography (HPLC) system manufactured by Waters Corporation. It is designed to separate, identify, and quantify components in a liquid sample. The E2695 system includes a solvent delivery module, an autosampler, and a column compartment to maintain temperature control. It is capable of performing a wide range of analytical techniques with a focus on precise and accurate sample handling and separation.

What are the different types or varieties of Aloe?

What are the common uses and benefits of Aloe?

Aloe has a long history of use in traditional and modern healthcare. The gel from Aloe leaves is commonly used to soothe and heal sunburns, minor cuts, and skin irritations. Aloe also contains compounds like anthraquinones and polysaccharides that have anti-inflammatory, antibacterial, and wound-healing properties. Some studies suggest Aloe may also have potential benefits for digestive health, diabetes management, and even cancer prevention, though more research is still needed.

How can PubCompare.ai help with Aloe research?

PubCompare.ai allows researchers to more efficiently screen protocol literature and leverage AI to pinpoint critical insights. The platform can help identify the most effective protocols related to Aloe for your specific research goals. PubCompare's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your Aloe studies.

What are some common challenges in using Aloe?

While Aloe is generally well-tolerated, some people may experience skin irritation or allergic reactions, especially with topical use. Ingesting Aloe can also cause side effects like diarrhea, abdominal cramps, and electrolyte imbalances, particularly with long-term or high-dose use. It's important to follow dosage instructions carefully and consult a healthcare provider, espeically before using Aloe for any serious medical condition.

More about "Aloe"

Aloe vera is a succulent plant genus renowned for its diverse medicinal properties.
This versatile plant has a rich history of use in traditional and modern healthcare.
PubCompare.ai, an AI-driven platform, empowers researchers to unlock the full potential of Aloe by providing access to the best protocols, products, and insights from scientific literature, preprints, and patents.
Aloe's active compounds, such as Gallic acid, can be extracted using analytical-grade methanol and NaHCO3.
Phosphoric acid may be used for pH adjustments.
Researchers can leverage the FLoid Cell Imaging Station to visualize and analyze Aloe-derived compounds.
In vivo studies on CD-1 mice have demonstrated the antioxidant and therapeutic effects of Aloe, as measured by DPPH assays.
Explore data comparisons on PubCompare.ai to optimize your Aloe research and ensure reproducibility.
Discover the latest breakthroughs in Aloe-based therapies, from wound healing to anti-inflammatory applications.
Unlock the future of Aloe research with the help of this powerful AI platform.
Diceover the true potential of this versatile plant today!