Grapes and grapefruit EPDENs were isolated and purified according to the methods as described previously[21 ]. For isolation and purification of ginger and carrot EPDENs, ginger and carrots were purchased from a local market and washed with water in a plastic bucket 3×, 1–3 min/each time at room temperature (22°C). After the final washing, the ginger or carrots were ground in a mixer to obtain the juice. The juice was sequentially centrifuged at 1000g for 10min, 3000g for 20 min and 10,000g for 40 min to remove large particles. The supernatant was then centrifuged at 150,000g for 90min, the pellet was resuspended in 1mL PBS and transferred to a sucrose step gradient (8%/15%/30%/45%/60%) and centrifuged at 150,000g for 120 min using a previously described protocol[22 ]. The bands between the 8%/30% layer and 30%/45% layer were harvested separately and noted as EPDENs. The protein concentration of the samples was determined using the Bio-Rad protein quantification assay kit (Bio-Rad, Hercules, CA). The purified specimens were prepared for EM using a conventional procedure[23 (link)] and observed using an FEI Tecnai F20 electron microscope operated at 200kV at a magnification of 38,000× and defocus of 2.5 µm. Photomicrographs were taken using a Gatan Ultrascan 4000 CCD camera.
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Citrus paradisi
Citrus paradisi
Citrus paradisi, commonly known as the grapefruit, is a subtropical citrus tree and its fruit.
Originating from Barbados, the grapefruit is a hybrid between the pamplemousse and the orange.
It is characterized by its large, yellow-skinned fruit with a distinctive bitter-sweet flavor.
Grapefruit is a popular and widely cultivated citrus species, valued for its nutritional properties and potential health benefits.
Reseachers can optimize their grapefruit-related studies using PubCompare.ai, an AI-driven platform that enhances reproducibility by locating relevant protocols from literature, preprints and patents, and providing AI-driven comparisons to identify the best protocols and products.
PubCompare.ai can help streamline grapefruit research and drive better outcomes.
Originating from Barbados, the grapefruit is a hybrid between the pamplemousse and the orange.
It is characterized by its large, yellow-skinned fruit with a distinctive bitter-sweet flavor.
Grapefruit is a popular and widely cultivated citrus species, valued for its nutritional properties and potential health benefits.
Reseachers can optimize their grapefruit-related studies using PubCompare.ai, an AI-driven platform that enhances reproducibility by locating relevant protocols from literature, preprints and patents, and providing AI-driven comparisons to identify the best protocols and products.
PubCompare.ai can help streamline grapefruit research and drive better outcomes.
Most cited protocols related to «Citrus paradisi»
Biological Assay
Carrots
Citrus paradisi
Daucus carota
Electron Microscopy
Grapes
isolation
Photomicrography
Proteins
Sucrose
Zingiber officinale
Biological Assay
Carrots
Centrifugation
Citrus paradisi
Curcuma longa root extract
Electron Microscopy
EMP1 protein, human
Exosomes
Filtration
Garlic
ginger root
Native Hawaiians
paraform
Pellets, Drug
Plants
Proteins
Serum Albumin, Bovine
Staphylococcal Protein A
Sucrose
Zingiber officinale
The DHD-index is a continuous score with ten components that represent the ten Dutch Guidelines for a Healthy Diet of 2006 (Table 1 ). By choosing a continuous scoring system we assume that we can observe changes in diets of intervention studies better than with a dichotomous scoring system. For all components a maximum of ten points can be allotted, resulting in a range of zero to 100 points. The components physical activity, vegetable, fruit, fish, and fiber are adequacy components and the components SFA, TFA, number of consumption occasions of ADF, sodium and alcohol are moderation components. Cut-off values represent the required amount of consumption or physical activities undertaken (minimum for adequacy and maximum for moderation components), whereas the threshold values represents the level of intake that deserves zero points for the moderation components. For the component ADF the threshold value was lower than the recommended maximum of seven ADF consumption occasions. Consequently, this component was scored dichotomously. The components and their cut-off and threshold values are shown in Table 1 .
The first component assesses physical activity; the Health Council of the Netherlands recommends being active for minimally 30 minutes of at least moderate intensity for at least five days per week [5 ]. The second component is based on the recommendation of 150–200 grams of vegetables per day. The higher of the two recommendations was chosen as the cut-off value of the component. The third component is based on the recommendation of 200 grams of fruits per day. The NNC communicates that a maximum of 100 grams can be replaced by fruit juices which naturally contain folate and vitamin C [22 ]. In the DNFCS-2003 six types of juice complied with the criterion (orange juice with and without pulp, pineapple juice, berry juice, grapefruit juice and mixed fruit juice) and could be included in the fruit group for a maximum of 100 grams in total. The fourth component is based on the recommendation of 30–40 grams of dietary fiber per day. The criterion used was stated in the background document and was 14 grams dietary fiber per 4.2mJ per day [15 ]. The fifth component, fish, is estimated based on the fish fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are likely to be the protective components of fish [15 ]. At least 450 mg/day of these fish fatty acids are recommended [15 ] and their intake can be achieved by fish consumption or by using fish oil capsules. Although, fish consumption is preferred by the Health Council of the Netherlands, fish oil capsules are permitted as substitute for fish for people who do not eat fish [23 ]. Fish oil capsules were assumed to contain 200 mg of fish fatty acids per capsule, based on labeling information of the fish oil capsules available in the Netherlands. The average daily intake of EPA and DHA from the capsules was added to the 2-day average intake of EPA and DHA from fish. The sixth and seventh components were based on the recommendations to consume less than 10 energy percent of SFA and less than one energy percent of TFA respectively. The eighth component is based on the maximum recommended number of ADF consumption occasions which is seven occasions per day including the three main meals. The operational definition of a ADF consumption occasion is every half an hour where a food item or drink with a pH level lower than 5.5 and a total acidity higher than 0.5% is consumed [24 (link)]. Consumption of less than 2.4 grams of sodium per day, as recommended in the corresponding guideline, is scored in component nine. In the DNFCS and most other studies, no data is available on salt added during cooking and at the table. The contribution of sodium of these sources was assumed to be on average about 30 % of total sodium intake, based on available literature [25 -27 ]. Therefore, we lowered the cut-off and threshold value for this component by 30%. The last component, alcohol, is differentiated by sex. For men, the recommendation is to consume maximally two Dutch units of alcohol, and for women to consume maximally one Dutch unit per day. One Dutch unit of alcohol contains 10 grams of ethanol [5 ].
The first component assesses physical activity; the Health Council of the Netherlands recommends being active for minimally 30 minutes of at least moderate intensity for at least five days per week [5 ]. The second component is based on the recommendation of 150–200 grams of vegetables per day. The higher of the two recommendations was chosen as the cut-off value of the component. The third component is based on the recommendation of 200 grams of fruits per day. The NNC communicates that a maximum of 100 grams can be replaced by fruit juices which naturally contain folate and vitamin C [22 ]. In the DNFCS-2003 six types of juice complied with the criterion (orange juice with and without pulp, pineapple juice, berry juice, grapefruit juice and mixed fruit juice) and could be included in the fruit group for a maximum of 100 grams in total. The fourth component is based on the recommendation of 30–40 grams of dietary fiber per day. The criterion used was stated in the background document and was 14 grams dietary fiber per 4.2mJ per day [15 ]. The fifth component, fish, is estimated based on the fish fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are likely to be the protective components of fish [15 ]. At least 450 mg/day of these fish fatty acids are recommended [15 ] and their intake can be achieved by fish consumption or by using fish oil capsules. Although, fish consumption is preferred by the Health Council of the Netherlands, fish oil capsules are permitted as substitute for fish for people who do not eat fish [23 ]. Fish oil capsules were assumed to contain 200 mg of fish fatty acids per capsule, based on labeling information of the fish oil capsules available in the Netherlands. The average daily intake of EPA and DHA from the capsules was added to the 2-day average intake of EPA and DHA from fish. The sixth and seventh components were based on the recommendations to consume less than 10 energy percent of SFA and less than one energy percent of TFA respectively. The eighth component is based on the maximum recommended number of ADF consumption occasions which is seven occasions per day including the three main meals. The operational definition of a ADF consumption occasion is every half an hour where a food item or drink with a pH level lower than 5.5 and a total acidity higher than 0.5% is consumed [24 (link)]. Consumption of less than 2.4 grams of sodium per day, as recommended in the corresponding guideline, is scored in component nine. In the DNFCS and most other studies, no data is available on salt added during cooking and at the table. The contribution of sodium of these sources was assumed to be on average about 30 % of total sodium intake, based on available literature [25 -27 ]. Therefore, we lowered the cut-off and threshold value for this component by 30%. The last component, alcohol, is differentiated by sex. For men, the recommendation is to consume maximally two Dutch units of alcohol, and for women to consume maximally one Dutch unit per day. One Dutch unit of alcohol contains 10 grams of ethanol [5 ].
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Ascorbic Acid
Berries
Capsule
Citrus paradisi
Dental Pulp
Dietary Fiber
Dietary Modification
Docosahexaenoic Acids
Eicosapentaenoic Acid
Ethanol
Fatty Acids
Fibrosis
Fishes
Folate
Food
Fruit
Fruit Juices
Heartburn
Oils, Fish
Pineapple
Sodium
Sodium Chloride, Dietary
Vegetables
Woman
Lipid samples extracted from either grapefruit or GNVs were submitted to the Lipidomics Research Center, Kansas State University (Manhattan, KS) for analysis. In brief, the lipid composition of GNVs was determined by using a triple quadrupole mass spectrometer (an Applied Biosystems Q-TRAP, Applied Biosystems, Foster City, CA). The protocol has been previously described35 (link). The data are reported as % of total signal for the molecular species determined after normalization of the signals to internal standards of the same lipid class.
Citrus paradisi
Lipids
In 1984 a 118 item food frequency questionnaire was sent to the participants of the Nurses’ Health Study to assess their habitual diet in the past year. In 1986 and every four years thereafter, a similar but expanded questionnaire was sent to the participants to update their dietary information. The expanded questionnaire was also administered every four years to assess diet among the participants in the Health Professionals Follow-up Study since 1986 and those in the Nurses’ Health Study II since 1991. In all food frequency questionnaires we asked the participants how often, on average, they consumed each food in a standard portion size. Participants could choose from nine possible responses, ranging from “never, or less than once per month” to “six or more times per day.” We consistently asked about 10 individual fruits since baseline: grapes or raisins; peaches, plums, or apricots; prunes; bananas; cantaloupe; apples or pears; oranges; grapefruit; strawberries; and blueberries. We calculated total whole fruit consumption by summing the consumption levels of the 10 individual fruits and watermelon, which we inquired about sporadically during follow-up. Fruit juice included apple, orange, grapefruit, and other juices. The food frequency questionnaires were validated against diet records among 173 participants in the Nurses’ Health Study in 1980 and 127 participants in the Health Professionals Follow-up Study in 1986.17 (link)
18 (link)
19 (link) Corrected correlation coefficients between food frequency questionnaire and diet record assessments of individual fruit consumption were 0.80 for apples, 0.79 for bananas, and 0.74 for oranges in women,17 (link) and 0.67 for total whole fruits, 0.76 for fruit juice, 0.95 for bananas, 0.84 for grapefruit, 0.76 for oranges, 0.70 for apples and pears, 0.59 for raisins and grapes, and 0.38 for strawberries in men.18 (link)
19 (link) For some individual fruits, the corrected correlation coefficients were not available owing to large within person variability in the comparison methods.
18 (link)
19 (link) Corrected correlation coefficients between food frequency questionnaire and diet record assessments of individual fruit consumption were 0.80 for apples, 0.79 for bananas, and 0.74 for oranges in women,17 (link) and 0.67 for total whole fruits, 0.76 for fruit juice, 0.95 for bananas, 0.84 for grapefruit, 0.76 for oranges, 0.70 for apples and pears, 0.59 for raisins and grapes, and 0.38 for strawberries in men.18 (link)
19 (link) For some individual fruits, the corrected correlation coefficients were not available owing to large within person variability in the comparison methods.
Apricot
Banana
Blueberries
Cantaloupes
Citrus paradisi
Diet
Food
Fruit
Fruit Juices
Grapes
Health Personnel
Healthy Volunteers
Nurses
Peach
Pears
Plum
Raisins
Strawberries
Watermelon
Woman
Most recents protocols related to «Citrus paradisi»
Naringenin and Naringenin
chalcone (purity ≥ 95%) are research-grade commercial products
(Merck Life Science S.r.l. Milan, Italy) and were used as received.
Italian cherry tomatoes (Solanum lycopersicum L.) cultivar “datterino” and red grapefruits (Citrus Paradisi) cultivar “Star Ruby”
were purchased from a local grocery store in Rome, Italy. Gaseous
ions [Nar + H]+ and [ChNar + H]+, both at m/z 273, were obtained by electrospray ionization mass spectrometry
(ESI-MS) in positive ion mode, through direct infusion by a syringe
pump of a solution (10 μM) of either Nar or ChNar in acidified
(1% HCOOH) methanol to favor protonation. Naringenin and Naringenin
chalcone has been extracted by maceration from grapefruit and tomato
peel.45 (link)−47 (link) Details of the tomato and grapefruit sample preparation
are described in theSupporting Information .
chalcone (purity ≥ 95%) are research-grade commercial products
(Merck Life Science S.r.l. Milan, Italy) and were used as received.
Italian cherry tomatoes (Solanum lycopersicum L.) cultivar “datterino” and red grapefruits (Citrus Paradisi) cultivar “Star Ruby”
were purchased from a local grocery store in Rome, Italy. Gaseous
ions [Nar + H]+ and [ChNar + H]+, both at m/z 273, were obtained by electrospray ionization mass spectrometry
(ESI-MS) in positive ion mode, through direct infusion by a syringe
pump of a solution (10 μM) of either Nar or ChNar in acidified
(1% HCOOH) methanol to favor protonation. Naringenin and Naringenin
chalcone has been extracted by maceration from grapefruit and tomato
peel.45 (link)−47 (link) Details of the tomato and grapefruit sample preparation
are described in the
Full text: Click here
Citrus paradisi
Lycopersicon esculentum
Methanol
naringenin
Prunus cerasus
Spectrometry, Mass, Electrospray Ionization
Tomatoes
Healthy white male participants were eligible for inclusion to the study if they were ≥ 40 years of age and ≤ 60 years of age and had a body mass index ≥ 18.0 kg/m2 and ≤ 29.9 kg/m2. Participants were excluded if they had a clinically relevant finding on the electrocardiogram (ECG), had systolic blood pressure (SBP) < 100 mmHg or > 145 mmHg, had diastolic blood pressure (DBP) < 60 mmHg or > 95 mmHg, or heart rate (HR) < 50 or > 95 beats per min (bpm). Regular use of medication within 4 weeks and any use of medication, herbal products, or vitamins within 14 days prior to the first study drug administration were prohibited. Regular daily consumption of more than ten cigarettes and intake of foods and beverages containing grapefruit within 14 days before study drug administration were exclusion criteria. A full list of inclusion and exclusion criteria is included in Table S1 of the Electronic Supplementary Material (ESM).
Beverages
Citrus paradisi
Eating
Electrocardiography
Healthy Volunteers
Index, Body Mass
Males
Pharmaceutical Preparations
Pressure, Diastolic
Rate, Heart
Systolic Pressure
Vitamins
Patients completed the fexofenadine pharmacokinetic study within 3 weeks of their endoscopy. They avoided consumption of any substance(s) which might have an impact on fexofenadine bioavailability, including grapefruit, orange and apple juices and fruits, tobacco, alcoholic drinks, medications (prescription and over-the-counter) and natural health products, for at least 48 hours before and during this study. All medications taken were documented before entry into the study. No patients were receiving a medication that was either an inhibitor or inducer of OATPs or P-glycoprotein. Testing was preceded by a 10-hour overnight fast. Fexofenadine 120 mg (Allegra, Hoechst Marion Roussel Inc, Laval, Quebec, Canada) was consumed with 300 mL water. Plasma samples were obtained at specified times (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 hours) relative to dosing for quantitation of fexofenadine concentrations. A gluten-free standardised lunch was provided 4 hours after drug dosing (noon).
Alcoholic Beverages
Allegra
Citrus paradisi
Drugs, Non-Prescription
Endoscopy, Gastrointestinal
fexofenadine
Fruit
Gluten-Free Diet
Natural Products
Organic Anion Transport Polypeptides
P-Glycoprotein
Patients
Pharmaceutical Preparations
Plasma
Tobacco Products
Tea leaves, rhubarb rhizome, white mustard, and black seeds were purchased as dried material, and aerial parts of R. graveolens, E. vesicaria, and A. officinalis, grapefruit peels, and grapes were dried in a vacuum-heated oven at 40 °C. All dried materials were powdered, and 50 g of each dried powder was repeatedly extracted with methanol (Adwic Co., Cairo, Egypt) until exhaustion (200 mL × 4) to obtain the methanolic extract. The fruits were peeled, thinly sliced, and dried in a vacuum oven for avocado. The dried fruits were powdered and then extracted with n-hexane (200 mL × 4, Adwic Co.) to obtain the fixed oil. Solvents were removed using a rotary evaporator, and the obtained residues were stored in amber-colored glass at 4 °C until use.
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Amber
Citrus paradisi
Fruit
Grapes
Methanol
n-hexane
Nigella sativa
Persea americana
Rhizome
Rhubarb
Sinapis alba
Solvents
Vacuum
Aerial parts of common rue (Ruta graveolens Linn., Rutaceae), Arugula (Eruca vesicaria (L.) Cav., Brassicaceae) garden asparagus (Asparagus officinalis L., Asparagaceae) were obtained from and authenticated at the medicinal, aromatic, and poisonous plants experimental station (MAPPES), Faculty of Pharmacy, Cairo University. Seeds of black seed (Nigella sativa L., Ranunculaceae) and white mustard (Sinapis alba L., Brassicaceae) were obtained from and authenticated by Agricultural Research Center, Giza, Egypt. Avocado fruits (Persea Americana Mill, Lauraceae, Duke cultivar), red grapes (Vitis vinifera L., Vitaceae, seedless variety), and grapefruits (Citrus paradisi Macfad, Rutaceae) were obtained from a private garden along the Cairo-Alexandria desert road. Dr. Ayman Elkafrawy authenticated plants at the Agricultural Research Center, Giza, Egypt. Voucher specimens are kept at the Herbarium of the Department of Pharmacognosy, Faculty of Pharmacy, Cairo University. The Dried rhubarb rhizome (Rheum officinale Baill, Polygonaceae) and dried green tea leaves (Camellia sinensis (L.) Kuntze, Theaceae) were obtained from a local herbal market in Cairo, Egypt. Dr. Reem Samir Hamdy authenticated them, Lecturer of Plant Taxonomy, Botany Department, Faculty of Science, Cairo University, Giza, Egypt. Plant material was further evaluated for possible contamination by pharmacognostic examination of their morphological and microscopical characters.
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Asparagaceae
Asparagus officinalis
Asparagus Plant
Brassicaceae
Camellia sinenses
Character
Citrus paradisi
Faculty
Faculty, Pharmacy
Fruit
Grapes
Green Tea
Lauraceae
Microscopy
Nigella sativa
Persea americana
Plants
Plants, Toxic
Polygonaceae
Ranunculaceae
Rheum officinale
Rhizome
Rhubarb
Rutaceae
Ruta graveolens
Sinapis alba
Theaceae
Vitaceae
Vitis
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More about "Citrus paradisi"
Grapefruit, Citrus paradisi, is a popular and widely cultivated citrus species known for its distinctive bitter-sweet flavor and potential health benefits.
Researchers can optimize their grapefruit-related studies using PubCompare.ai, an AI-driven platform that enhances reproducibility by locating relevant protocols from literature, preprints and patents, and providing AI-driven comparisons to identify the best protocols and products.
PubCompare.ai can help streamline grapefruit research by providing access to a wide range of protocols and methods, including those involving key techniques and equipment such as Acetonitrile, Whatman No. 1 filter paper, Zetasizer Nano ZS, Beckman Optima L-90K, Caspase-9 Colorimetric Assay Kit, Microplate reader, DNeasy Plant Mini Kit, Sodium hydroxide, Mold Star™ 30 silicone, and HLP-10 demineralizer.
This can help researchers save time and improve the reliability of their experiments, ultimately leading to better outcomes in their Citrus paradisi studies.
By using PubCompare.ai, researchers can ensure their grapefruit-related research is more reproducible, efficient, and effective, leveraging the power of AI to streamline their workflows and drive innovative discoveries.
Whether investigating the nutritional properties, potential health benefits, or other aspects of this versatile citrus fruit, PubCompare.ai can be a valuable tool in the researcher's arsenal.
Researchers can optimize their grapefruit-related studies using PubCompare.ai, an AI-driven platform that enhances reproducibility by locating relevant protocols from literature, preprints and patents, and providing AI-driven comparisons to identify the best protocols and products.
PubCompare.ai can help streamline grapefruit research by providing access to a wide range of protocols and methods, including those involving key techniques and equipment such as Acetonitrile, Whatman No. 1 filter paper, Zetasizer Nano ZS, Beckman Optima L-90K, Caspase-9 Colorimetric Assay Kit, Microplate reader, DNeasy Plant Mini Kit, Sodium hydroxide, Mold Star™ 30 silicone, and HLP-10 demineralizer.
This can help researchers save time and improve the reliability of their experiments, ultimately leading to better outcomes in their Citrus paradisi studies.
By using PubCompare.ai, researchers can ensure their grapefruit-related research is more reproducible, efficient, and effective, leveraging the power of AI to streamline their workflows and drive innovative discoveries.
Whether investigating the nutritional properties, potential health benefits, or other aspects of this versatile citrus fruit, PubCompare.ai can be a valuable tool in the researcher's arsenal.