Eucalyptus
These trees are known for their distinctive bark, leaves, and flowers, and have a wide range of uses in industries such as timber, pulp, and essential oils.
Eucalyptus trees are also important in many ecosystems, providing habitat and resources for a variety of wildlife.
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Most cited protocols related to «Eucalyptus»
Food frequency questionnaire (FFQ), which was taken from previously validated questionnaires [24 –26 (link)], containing 54 food items was used to collect dietary data. The questionnaire was also validated after the assessment of locally available foodstuffs. For the food frequency questions, the women were asked about the frequency of consumption of each food per day, per week or per month in the prior 3 months by taking the variation of dietary intakes within days of the week into consideration [25 (link), 26 (link)].
Food items of the FFQs were grouped into nine food groups: 1. cereals, roots, and tubers; 2. Vitamin-A-rich fruits and vegetables; 3. other fruits; 4. other vegetables; 5. legumes and nuts; 6. meat, poultry, and fish; 7. fats and oils; 8.dairy; and 9. Eggs [24 ].The consumers of a food item were defined as the consumption of a food item at least once a week [26 (link)]. The number of food groups the women ate within a week were counted to analyze dietary diversity score (DDS).
Food variety score (FVS) was computed by counting the individual food items the women consumed within a week. Then, the mean FVS was analyzed. The utilization of animal source food (ASF) was assessed by counting the frequency of each animal source foods the women took within the days of a week. Finally, the frequency of ASF consumption was divided into terciles (three parts). Dietary diversity score, FVS, ASF consumption, and frequency of meal were used to assess dietary practices.
The food security status of the household was assessed using 27 questions, which were adapted from the household food insecurity access scale. The questions were previously validated for use in developing countries [27 (link)]. Food secure households experienced fewer than the first 2 food insecurity indicators. Whereas, a household which experienced from 2 to 10, 11–17, and > 17 food insecurity indicators were considered as mildly, moderately, and severely food insecure households, respectively.
The wealth index of the household was determined using Principal Component Analysis (PCA) by considering latrine, water source, household assets, livestock, and agricultural land ownership. The responses of all non-dummy variables were classified into three parts. The highest score was coded as 1. Whereas, the two lower values were given code 0. In PCA, those variables having a commonality value of greater than 0.5 were used to produce factor scores. Lastly, the score for each household on the first principal component was retained to create the wealth score. Quintiles of the wealth score were created to categorize households as poorest, poor, medium, rich, and richest.
To determine edible crop and vegetable production, each crop and vegetable species cultivated by the household were counted. The number of crops and vegetables produced was classified into three parts. The highest value was labeled as high production, while the two lower values were considered as low production. In the study area, khat, eucalyptus, “Gesho” (a local plant used to make tella or local beer and areki), pepper, and onion are the common cash crops. Count of these cash crops was used to decide cash crop production.
The total ownership of livestock was measured by Tropical Livestock Units (TLUs). There is no TLU index created specifically for Ethiopia, therefore, the indexes for Tropical Africa was used in this study. The TLUs were calculated using the following weighted index factors: cattle = 0.7, horses = 0.5, mules = 0.5, donkey = 0.5, sheep = 0.1, goats = 0.1, chickens = 0.01 [28 ].
Women’s autonomy was assessed using eight questions. For each question, code one was given when a decision was made by the woman alone or jointly with her husband, otherwise zero. The mean was used to classify a woman’s decision making power [16 ].
Maternal knowledge on diet during pregnancy was assessed using 12 questions. Code one was given for each question when the response was correct, or else zero. The attitude was assessed by 20 Likert scale questions using PCA. The factor scores were summed and ranked into terciles (three parts). Then the highest tercile was labeled as a favorable attitude, if not unfavorable attitude.
Subjective norms, intention, perceived susceptibility, perceived severity, perceived benefit, and perceived barriers were assessed using their respective composite questions. Mean was computed and women who scored above the mean for each variable were categorized as having subjective norms, intention, perceived susceptibility, perceived severity, perceived benefit, and perceived barriers, otherwise no.
(1990 ) was used to isolate
genomic DNA from fungal mycelium, grown on MEA in Petri dishes. The primers
ITS1 and ITS4 (
1990
spanning the 3' end of the 18S rRNA gene, the first internal transcribed
spacer (ITS1), the 5.8S rRNA gene, the second ITS region and the 5' end of the
28S rRNA gene. The PCR reaction mixture and conditions were the same as those
used by Crous et al.
(2004b ).
The ITS nucleotide sequences generated in this study were added to other
sequences obtained from GenBank
(
and the alignment was assembled using Sequence Alignment Editor v. 2.0a11
(Rambaut 2002 ) with manual
adjustments for visual improvement where necessary. Due to the size and the
complexity of the original alignment, the sequences were split over four
smaller alignments, each containing genetically similar sequences. The four
datasets were each treated identically. Phylogenetic analyses of sequence data
were done using PAUP (Phylogenetic Analysis Using Parsimony) v. 4.0b10
(Swofford 2002 ). Phylogenetic
analysis of the aligned ITS sequence data consisted of neighbour-joining
analysis with the uncorrected (“p”), the Kimura 2-parameter and
the HKY85 substitution model in PAUP. Alignment gaps were treated as missing
data and all characters were unordered and of equal weight. When they were
encountered, ties were broken randomly. Sequence data were deposited in
GenBank (
alignments in TreeBASE.
Mycosphaerella and anamorph isolates included in this study for
sequence analysis and morphological comparison.
no. | ||||||
---|---|---|---|---|---|---|
Mycosphaerella communis | Dissoconium commune | CPC 11700 | Eucalyptus globulus | Spain | P. Mansilla | DQ302948 |
CPC 11703 | Eucalyptus globulus | Spain | P. Mansilla | DQ302949 | ||
CPC 11792 | Eucalyptus sp. | Portugal | A.J.L. Phillips | DQ302950 | ||
Mycosphaerella cryptica | Colletogloeopsis nubilosum | 1576 | Eucalyptus nitens | Australia | M.J. Wingfield | DQ302951 |
Mycosphaerella endophytica | Pseudocercosporella endophytica | 1191 | Eucalyptus sp. | South Africa | P.W. Crous | DQ302952 |
1193 | Eucalyptus | South Africa | P.W. Crous | DQ302953 | ||
Mycosphaerella eucalyptorum | — | 11174 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ302954 |
Mycosphaerella flexuosa | Stenella sp. | Eucalyptus globulus | Colombia | M.J. Wingfield | DQ302955 | |
1200 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ302956 | ||
1201 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ302957 | ||
CPC 10995 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ302958 | ||
Mycosphaerella gamsii | — | 11138 | Eucalyptus sp. | India | W. Gams | DQ302959 |
Mycosphaerella gracilis | Pseudocercospora gracilis | 1315 | Eucalyptus urophylia | Indonesia | M.J. Wingfield | DQ302960 |
CPC 11144 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ302961 | ||
CPC 11181 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ302962 | ||
Mycosphaerella heimii | Pseudocercospora heimii | CPC 11441 | Eucalyptus sp. | Brazil | A.C. Alfenas | DQ302963 |
CPC 11453 | Eucalyptus sp. | Brazil | A.C. Alfenas | DQ302964 | ||
CPC 11548 | Eucalyptus sp. | Brazil | A.C. Alfenas | DQ302965 | ||
CPC 11716 | — | Brazil | A.C. Alfenas | DQ302966 | ||
CPC 11879 | Eucalyptus sp. | Portugal | A.J.L. Phillips | DQ302967 | ||
Mycosphaerella jonkershoekensis | — | 3116 | Protea lepidocarpodendron | Australia | P.W. Crous | DQ302968 |
Mycosphaerella lateralis | Dissoconium dekkeri | CPC 11218 | Eucalyptus comaldulensis | Bolivia | M.J. Wingfield | DQ302969 |
CPC 11293 | Eucalyptus tereticornis | Bolivia | M.J. Wingfield | DQ302970 | ||
CPC 11484 | Eucalyptus sp. | Spain | P. Mansilla | DQ302971 | ||
CPC 11706 | Eucalyptus globulus | Spain | P. Mansilla | DQ302972 | ||
CPC 11729 | Eucalyptus globulus | Spain | P. Mansilla | DQ302973 | ||
CPC 11732 | Eucalyptus globulus | Spain | P. Mansilla | DQ302974 | ||
CPC 11789 | Eucalyptus sp. | Portugal | J.P. Sampaio | DQ302975 | ||
Mycosphaerella madeirae | — | CPC 3746 | Eucalyptus grandis | Madeira | S. Denman | DQ302976 |
Mycosphaerella marksii | ? Pseudocercospora epispermogoniana | 1073 | Eucalyptus sp. | Tanzania | M.J. Wingfield | DQ302977 |
1499 | Eucalyptus globulus | Uruguay | M.J. Wingfield | DQ302978 | ||
5358 | Leucadendron tinctum | Madeira | S. Denman | DQ302979 | ||
3715 | Eucalyptus deglupha | Ecuador | M.J. Wingfield | DQ302980 | ||
CPC 11215 | Eucalyptus comaldulensis | Bolivia | M.J. Wingfield | DQ302981 | ||
CPC 11221 | Eucalyptus grandis | Bolivia | M.J. Wingfield | DQ302982 | ||
CPC 11222 | Eucalyptus grandis | Bolivia | M.J. Wingfield | DQ302983 | ||
CPC 11795 | Vepris reflexa | South Africa | P.W. Crous | DQ302984 | ||
Mycosphaerella molleriana | Colletogloeopsis molleriana | CPC 11187 | Eucalyptus sp. | Spain | M.J. Wingfield | DQ302985 |
CPC 11685 | Eucalyptus globulus | Spain | P. Mansilla | DQ302986 | ||
CPC 11688 | Eucalyptus globulus | Spain | P. Mansilla | DQ302987 | ||
CPC 11709 | Eucalyptus globulus | Spain | P. Mansilla | DQ302988 | ||
CPC 11842 | Eucalyptus sp. | Portugal | A.J.L. Phillips | DQ302989 | ||
CPC 11845 | Eucalyptus sp. | Portugal | A.J.L. Phillips | DQ302990 | ||
CPC 12056 | Eucalyptus sp. | Uruguay | M.J. Wingfield | DQ302991 | ||
Mycosphaerella nubilosa | ? Uwebraunia juvenis | CPC 11246 | Eucalyptus globulus | Spain | M.J. Wingfield | DQ302992 |
CPC 11249 | Eucalyptus globulus | Spain | M.J. Wingfield | DQ302993 | ||
CPC 11487 | Eucalyptus sp. | Spain | P. Mansilla | DQ302994 | ||
CPC 11559 | Eucalyptus sp. | Spain | P. Mansilla | DQ302995 | ||
CPC 11723 | Eucalyptus globulus | Portugal | A.C. Alfenas | DQ302996 | ||
CPC 11761 | Eucalyptus globulus | Spain | P. Mansilla | DQ302997 | ||
CPC 11767 | Eucalyptus globulus | Portugal | L.P. Phillips | DQ302998 | ||
CPC 11882 | Eucalyptus globulus | Portugal | A.J.L. Phillips | DQ302999 | ||
CPC 11885 | Eucalyptus sp. | Portugal | A.J.L. Phillips | DQ303000 | ||
Mycosphaerella parva | — | CPC 11273 | Eucalyptus globulus | Spain | M.J. Wingfield | DQ303001 |
CPC 11758 | Eucalyptus globulus | Spain | P. Mansilla | DQ303002 | ||
CPC 11759 | Eucalyptus globulus | Spain | P. Mansilla | DQ303003 | ||
CPC 11764 | Eucalyptus globulus | Spain | P. Mansilla | DQ303004 | ||
CPC 11888 | Eucalyptus sp. | Portugal | A.J.L. Phillips | DQ303005 | ||
Mycosphaerella perpendicularis | — | 10983 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303006 |
Mycosphaerella pluritubularis | — | 11697 | Eucalyptus globulus | Spain | P. Mansilla | DQ303007 |
Mycosphaerella pseudafricana | — | 1230 | Eucalyptus globulus | Zambia | T.A. Coutinho | DQ303008 |
Mycosphaerella pseudocryptica | Colletogloeopsis sp. | CPC 11264 | Eucalyptus sp. | New Zealand | J.A. Stalpers | DQ303009 |
11267 | Eucalyptus sp. | New Zealand | J.A. Stalpers | DQ303010 | ||
Mycosphaerella pseudosuberosa | Trimmatostroma sp. | 12085 | Eucalyptus sp. | Uruguay | M.J. Wingfield | DQ303011 |
Mycosphaerella quasicercospora | — | 1098 | Eucalyptus sp. | Tanzania | M.J. Wingfield | DQ303012 |
Mycosphaerella readeriellophora | Readeriella readeriellophora | CPC 11711 | Eucalyptus globulus | Spain | P. Mansilla | DQ303013 |
Mycosphaerella scytalidii | — | Eucalyptus globulus | Brazil | F.A. Ferreira | DQ303014 | |
CPC 10988 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303015 | ||
10998 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303016 | ||
Mycosphaerella secundaria | — | 1112 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303017 |
Eucalyptus grandis | Brazil | A.C. Alfenas | DQ303018 | |||
CPC 10989 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303019 | ||
11551 | Eucalyptus sp. | Brazil | A.C. Alfenas | DQ303020 | ||
Mycosphaerella sp. | Stenella pseudoparkii | 1090 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303021 |
1092 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303022 | ||
1087 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303023 | ||
1088 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303024 | ||
1089 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303025 | ||
Mycosphaerella sp. | Stenella xenoparkii | 1299 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303026 |
1301 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303027 | ||
1300 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303028 | ||
Mycosphaerella sp. | — | 727 | Eucalyptus grandis | Indonesia | A.C. Alfenas | DQ303029 |
Mycosphaerella sp. | — | 728 | Eucalyptus grandis | Indonesia | A.C. Alfenas | DQ303030 |
Mycosphaerella sp. | — | Eucalyptus globulus | Brazil | F.A. Ferreira | DQ303031 | |
Mycosphaerella sp. | — | Eucalyptus globulus | Brazil | F.A. Ferreira | DQ303032 | |
Mycosphaerella sp. | — | Eucalyptus globulus | Brazil | F.A. Ferreira | DQ303033 | |
Mycosphaerella sp. | — | 1093 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303034 |
Mycosphaerella sp. | — | 1091 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303035 |
Mycosphaerella sp. | — | 1101 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303036 |
Mycosphaerella sp. | — | CPC 10986 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303037 |
Mycosphaerella sp. | — | CPC 11002 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303038 |
Mycosphaerella sp. | — | CPC 11004 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303039 |
Mycosphaerella sp. | — | CPC 12200 | Eucalyptus sp. | South Africa | Z.A. Pretorius | DQ303040 |
Mycosphaerella sp. | — | CPC 12147 | Acacia mangium | Thailand | W. Himaman | DQ303041 |
Mycosphaerella stramenti | — | 11545 | Eucalyptus sp. | Brazil | A.C. Alfenas | DQ303042 |
Mycosphaerella stramenticola | — | 11438 | Eucalyptus sp. | Brazil | A.C. Alfenas | DQ303043 |
Mycosphaerella suberosa | — | CPC 11032 | Eucalyptus sp. | Colombia | M.J. Wingfield | DQ303044 |
CPC 11190 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303045 | ||
CPC 11276 | Eucalyptus comaldulensis | Spain | M.J. Wingfield | DQ303046 | ||
CPC 12193 | Eucalyptus sp. | — | A.C. Alfenas | DQ303047 | ||
Mycosphaerella sumatrensis | — | 11171 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303048 |
11175 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303049 | ||
11178 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303050 | ||
Mycosphaerella suttonii | Kirramyces epicoccoides | 1550 | Eucalyptus grandis | Australia | M.J. Wingfield | DQ303051 |
1409 | Eucalyptus sp. | Brazil | P.W. Crous | DQ303052 | ||
Eucalyptus grandis | South Africa | P.W. Crous | DQ303053 | |||
1581 | Eucalyptus grandis | Australia | M.J. Wingfield | DQ303054 | ||
CPC 11279 | Eucalyptus tereticornis | Bolivia | M.J. Wingfield | DQ303055 | ||
Mycosphaerella verrucosiafricana | — | 11167 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303056 |
11169 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303057 | ||
11170 | Eucalyptus sp. | Indonesia | M.J. Wingfield | DQ303058 | ||
Mycosphaerella vespa | Colletogloeopsis sp. | CMW 11558 | Eucalyptus sp. | Australia | — | DQ303059 |
CMW 11559 | Eucalyptus sp. | Australia | — | DQ303060 | ||
CMW 11560 | Eucalyptus sp. | Australia | — | DQ303061 | ||
CMW 11563 | Eucalyptus sp. | Australia | — | DQ303062 | ||
CMW 11564 | Eucalyptus sp. | Australia | — | DQ303063 | ||
Mycosphaerella walkeri | Sonderhenia eucalypticola | CPC 11252 | Eucalyptus globulus | Spain | M.J. Wingfield | DQ303064 |
— | Colletogloeopsis zuluensis | CPC 11780 | Eucalyptus sp. | South Africa | P.W. Crous | DQ303065 |
CPC 11783 | Eucalyptus sp. | South Africa | P.W. Crous | DQ303066 | ||
CPC 11962; CMW 17322 | Eucalyptus sp. | South Africa | M.J. Wingfield | DQ303067 | ||
CPC 11965; CMW 17326 | Eucalyptus sp. | Uruguay | M.J. Wingfield | DQ303068 | ||
CPC 12059 | Eucalyptus sp. | Uruguay | M.J. Wingfield | DQ303069 | ||
— | Colletogloeopsis sp. | CPC 11786 | Eucalyptus sp. | South Africa | P.W. Crous | DQ303070 |
— | Pseudocercospora basitruncata | 1202 | Eucalyptus grandis | Colombia | M.J. Wingfield | DQ303071 |
— | Pseudocercospora clematidis | CPC 11657 | Clematis sp. | U.S.A. | M.A. Palm | DQ303072 |
— | Pseudocercospora epispermogoniana | Eucalyptus grandis | South Africa | G. Kemp | DQ303073 | |
— | Eucalyptus grandis | South Africa | G. Kemp | DQ303074 | ||
Eucalyptus grandis | South Africa | G. Kemp | DQ303075 | |||
— | Pseudocercospora fatouae | CPC 11648 | Fatoua villosa | Korea | H.D. Shin | DQ303076 |
— | Pseudocercospora natalensis | 1263 | Eucalyptus nitens | South Africa | T.A. Coutinho | DQ303077 |
— | Pseudocercospora pseudoeucalyptorum | 3751 | Eucalyptus sp. | Madeira | S. Denman | DQ303078 |
CPC 10916 | Eucalyptus sp. | South Africa | P.W. Crous | DQ303079 | ||
CPC 11713 | Eucalyptus globulus | Spain | P. Mansilla | DQ303080 | ||
— | Pseudocercospora robusta | 1269 | Eucalyptus robur | Malaysia | M.J. Wingfield | DQ303081 |
— | Pseudocercospora sp. | 1266 | Eucalyptus pellita | Thailand | M.J. Wingfield | DQ303082 |
1493 | Eucalyptus globulus | Uruguay | M.J. Wingfield | DQ303083 | ||
CPC 11591 | Brachybotrys paridiformis | Korea | H.D. Shin | DQ303084 | ||
CPC 11592 | Zelkova serrata | Korea | H.D. Shin | DQ303085 | ||
CPC 11654 | Morus bombycis | Korea | H.D. Shin | DQ303086 | ||
CPC 11668 | Pilea hamaoi | Korea | H.D. Shin | DQ303087 | ||
CPC 11680 | Ampelopsis brevipenduncula var. heterophylla | Korea | H.D. Shin | DQ303088 | ||
CPC 11726 | Platanus occidentalis | Korea | H.D. Shin | DQ303089 | ||
— | Pseudocercospora subulata | 10849 | Eucalyptus botryoides | New Zealand | M. Dick | DQ303090 |
— | Pseudocercosporella capsellae | CPC 11677 | Draba nemorosa var. hebecarpa | Korea | H.D. Shin | DQ303091 |
— | Readeriella sp. | CPC 11186 | Eucalyptus globulus | Spain | M.J. Wingfield | DQ303092 |
CPC 11735 | Eucalyptus globulus | Spain | P. Mansilla | DQ303093 | ||
— | Readeriella mirabilis | CPC 11712 | Eucalyptus globulus | Spain | P. Mansilla | DQ303094 |
— | Septoria eucalyptorum | 11282 | Eucalyptus sp. | India | W. Gams | DQ303095 |
— | Septoria provencialis | 12226 | Eucalyptus sp. | France | P.W. Crous | DQ303096 |
— | Stenella sp. | CPC 11671 | Lonicera japonica | Korea | H.D. Shin | DQ303097 |
CBS: Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands; CPC:
Culture collection of Pedro Crous, housed at CBS; CMW: Culture collection of
Mike Wingfield, housed at FABI, Pretoria, South Africa
Phylogenetic analyses using maximum parsimony (MP) and maximum likelihood (ML) were performed using PAUP* version 4.10 [80 ] on two data sets, one including 28 taxa and a second including 29 taxa by the addition of Gossypium. Phylogenetic analyses excluded gap regions. All MP searches included 100 random addition replicates and TBR branch swapping with the Multrees option. Modeltest 3.7 [81 (link)] was used to determine the most appropriate model of DNA sequence evolution for the combined 61-gene dataset. Hierarchical likelihood ratio tests and the Akaikle information criterion were used to assess which of the 56 models best fit the data, which was determined to be GTR + I + Γ by both criteria. For ML analyses we performed an initial parsimony search with 100 random addition sequence replicates and TBR branch swapping, which resulted in a single tree. Model parameters were optimized onto the parsimony tree. We fixed these parameters and performed a ML analysis with three random addition sequence replicates and TBR branch swapping. The resulting ML tree was used to re-optimize model parameters, which then were fixed for another ML search with three random addition sequence replicates and TBR branch swapping. This successive approximation procedure was repeated until the same tree topology and model parameters were recovered in multiple, consecutive iterations. This tree was accepted as the final ML tree (Figs.
Most recents protocols related to «Eucalyptus»
Example 3
A study is presented herein which demonstrates the redispersion effect of the fibre composition of the invention.
Tested formulations represent MFC fibre compositions without whitened eucalyptus kraft cellulose additivation; compositions of MFC fibres additivated with 5%, 10% and 20% of whitened eucalyptus kraft cellulose; and formulation with 100% of cellulose.
The morphological and mechanical properties of the formulations were analyzed before and after the pressing step.
The morphological properties analyzed were: fines content (%), fibre length (mm), fibre width (μm) and number of fibres per mass of the composition (millions of fibres/gram).
The analyzed mechanical properties were: tensile index (Nm/g), elongation (%), bursting index (KPam2/g), Scott Bond (ft·lb/in2), body, also referred to as volume-to-mass ratio, (cm3/g) and air passage resistance (s/100 mL air).
The obtained results are presented in the graphs from
Through the obtained results, it is concluded that there is retention of cellulose in the MFC maintaining the properties of the fibre proportion in the composition with regard to its morphology. Furthermore, no significant differences were observed in formulations before and after pressing.
pulp, unrefined (15 °SR), was provided by Ence (Navia, Spain).
2,2,6,6-Tetramethylpiperidine-1-oxy radical (TEMPO), NaBr, NaOH, NaClO
(15%), copper(II) ethylenediamine, and DTZ (≥98%) were purchased
from Sigma-Aldrich (Schnelldorf, Germany). Glacial acetic acid was
purchased from Scharlab (Sentmenat, Barcelona, Spain). All organic
solvents (reagent grade) were received from Thermo Fisher Scientific
(Loughborough, U.K.). Preliminary results indicated that amylene-stabilized
chloroform is preferred over ethanol-stabilized chloroform.
Distilled water was used for nanocellulose production, but metal
salts were dissolved in Milli-Q water. These metal salts were lead(II)
nitrate, lead(II) chloride, cadmium(II) nitrate, cadmium(II) chloride,
copper(II) chloride, nickel(II) chloride, chromium(III) chloride,
chromium(III) nitrate, and magnesium chloride from Panreac Applichem
(Castellar del Vallès, Barcelona, Spain); potassium nitrate,
iron(III) chloride, and manganese(II) chloride from Scharlab; and
mercury(II) nitrate 1-hydrate, mercury(II) chloride, silver nitrate,
and zinc chloride from Sigma-Aldrich.
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
To examine the evolutionary history of TPS genes, a second analysis including more species (E. grandis, E. globulus, A. thaliana, P. trichocarpa, V. vinifera, C. citriodora, and M. alternifolia) was carried out. We generated a tree with TPS sequences related to primary metabolism (subfamilies -c, -e, and -f) with a total of 45 sequences and a second tree related to secondary metabolism (subfamilies a, b, g) including 360 sequences29 (link),32 (link),55 (link).
The functionally characterized pinene (RtTPS1 and RtTPS2 accession number AXY92166 and AXY92167, respectively) and caryophyllene synthases (RtTPS3 and RtTPS4 accession numbers AXY92168 and AXY92169) from Rhodomyrtus tomentosa52 (link), pinene synthase (EpTPS1 accession number MK873024) and 1,8-cineole synthases (EpTPS2 and EpTPS3 accession numbers MK873025 and QCQ05478) from Eucalyptus polybractea56 (link), beta cayophyllene synthase (Eucgr. J01451) from E. grandis29 (link), myrcene synthase from Antirrhium majus (AAO41727)101 (link), two isoprene synthase genes from E. globulus (EglobTPS106), E. grandis (Eucgr. K00881)29 (link) and five linalool synthases from Oenothera californica (AAD19841)63 (link), Clarkia breweri (AAD19840), Clarkia concinna (AAD19839), and Fragaria x ananassa (CAD57106)102 (link) were also included in the phylogenetic analysis to assess the homology of known TPS to Psidium genes.
For each dataset used to construct the trees, we first aligned the amino acid sequences of putative TPS genes using ClustalW implemented within MEGA v7.0 software package103 (link). Due to high levels of variation and variable exon counts between taxa, we trimmed the alignment using Gblocks104 (link) with the following parameters: smaller final blocks, gap positions within the final blocks, and less strict flanking positions. We used the maximum-likelihood method implemented in PhyML v2.4.4105 (link) online web server106 (link) to perform the phylogenetic analysis. The JTT + G + F was the best-fit substitution model selected with ModelGenerator for protein analyses107 (link). The confidence values in the tree topology were assessed by running 100 bootstrap replicates. Trees were visualized using Figtree v1.4.4108 .
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