Glycyrrhiza uralensis was used as an example to introduce the comparative genomic approach to predict the GO annotation. We use BLAST-2.2.19 (-e 1e-3 -m 8) to compare the protein sequences of G. uralensis (34 (link)) and Arabidopsis thaliana (35 (link)). Then, the orthologous pairs with A. thaliana were determined, as was the corresponding GO annotation. Additionally, Blast2GO has a comprehensive bioinformatics platform that can automatically predict GO annotation based on the sequence information and a powerful remote annotation background (36 (link)). After the BLAST, interpro and mapping analyses steps in the Blast2GO application, we can export the GO annotation file and update agriGO v2.0. The InterPro (37 (link)) ID and Pfam (38 (link)) accessions have internal connections with the GO. Thus, we can map the GO terms to candidate genes using information on specific domains.
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Glycyrrhiza uralensis
Glycyrrhiza uralensis
Glycyrrhiza uralensis, also known as the Chinese licorice, is a perennial herb native to Central Asia.
It has a long history of use in traditional Chinese medicine, where its roots are valued for their various therapeutic properties.
This plant has gained significant attention in the scientific community due to its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects.
Reserach into Glycyrrhiza uralensis is crucial for understanding its pharmacological potential and developing new therapeutic applications.
PubCompare.ai's revolutionary AI-driven protocol comparison tool can help streamline this research by enhacing reproducibility and accuracy, allowing researchers to easily identify the best protocols and products from literature, preprints, and patents.
Utilizing this innovative platform can take your Glycyrrhiza uralensis studies to the next level and accelerate the discovery of new and effective applications for this remarkable medicinal plant.
It has a long history of use in traditional Chinese medicine, where its roots are valued for their various therapeutic properties.
This plant has gained significant attention in the scientific community due to its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects.
Reserach into Glycyrrhiza uralensis is crucial for understanding its pharmacological potential and developing new therapeutic applications.
PubCompare.ai's revolutionary AI-driven protocol comparison tool can help streamline this research by enhacing reproducibility and accuracy, allowing researchers to easily identify the best protocols and products from literature, preprints, and patents.
Utilizing this innovative platform can take your Glycyrrhiza uralensis studies to the next level and accelerate the discovery of new and effective applications for this remarkable medicinal plant.
Most cited protocols related to «Glycyrrhiza uralensis»
Glycyrrhiza uralensis was used as an example to introduce the comparative genomic approach to predict the GO annotation. We use BLAST-2.2.19 (-e 1e-3 -m 8) to compare the protein sequences of G. uralensis (34 (link)) and Arabidopsis thaliana (35 (link)). Then, the orthologous pairs with A. thaliana were determined, as was the corresponding GO annotation. Additionally, Blast2GO has a comprehensive bioinformatics platform that can automatically predict GO annotation based on the sequence information and a powerful remote annotation background (36 (link)). After the BLAST, interpro and mapping analyses steps in the Blast2GO application, we can export the GO annotation file and update agriGO v2.0. The InterPro (37 (link)) ID and Pfam (38 (link)) accessions have internal connections with the GO. Thus, we can map the GO terms to candidate genes using information on specific domains.
Amino Acid Sequence
Arabidopsis thalianas
Base Sequence
FCER2 protein, human
Genes
Glycyrrhiza uralensis
acetonitrile
Butyl Alcohol
Chromatography
Diagnosis
Glycyrrhiza uralensis
High-Performance Liquid Chromatographies
Medicines, Herbal
Methanol
Retention (Psychology)
Solvents
A total of 18 batches of HQT were included in the present study. Four batches coded as PHY906-6, 7, 8, 10 were manufactured with PhytoCeutica's proprietary SOP. Eight batches of HQT were purchased from Sun Ten Pharmaceutical Co. LTD in Taiwan and designated as HQT-E, F, G, H, I, J, K and L. Six batches of HQT were obtained from various vendors (Chung Song Zong, Ko Da, Min Tong, Sheng Chang, Sheng Foong, Kaiser; Taiwan) who did not provide quality information, and were labeled as HQT-CSZ, KD, MT, SC, SF and KP3. The proprietary standard operating procedures (SOP) by PhytoCeutica for PHY906 used hot water extraction (80°C) of four herbs, namely Scutellaria baicalensis Georgi (S), Paeonia lactiflora Pall. (P), Glycyrrhiza uralensis Fisch. (G) and Ziziphus jujuba Mill. (Z) (ratio 3:2:2:2). The hot water extraction is then spray dried with insoluble dextran into a granulated powder, packaged and stored in foil containers at 4°C.
Chemical standards including baicalin (S), baicalein (S), wogonin (S), scutellarin (S), glycyrrhizin (G), ononin (G), liquiritin (G), liqiritigenin (G), paeoniflorin (P) and albiflorin (P), were obtained from Chromadex (USA). Apigenin and formic acid were obtained from Sigma-Aldrich (USA). Solvents were of LC/MS grade from JT Baker (USA).
Chemical standards including baicalin (S), baicalein (S), wogonin (S), scutellarin (S), glycyrrhizin (G), ononin (G), liquiritin (G), liqiritigenin (G), paeoniflorin (P) and albiflorin (P), were obtained from Chromadex (USA). Apigenin and formic acid were obtained from Sigma-Aldrich (USA). Solvents were of LC/MS grade from JT Baker (USA).
albiflorin
Apigenin
baicalein
baicalin
Dextran
formic acid
Glycyrrhiza uralensis
Glycyrrhizic Acid
Huangqin
liquiritin
ononin
Paeonia
peoniflorin
Pharmaceutical Preparations
PHY 906
Powder
scutellarin
Solvents
wogonin
Ziziphus
The XYS formula was composed of eight herbal medicines. The composition and dose of the prescription is listed in Table 1 . The raw herbs were obtained from the Tongrentang (Bozhou, Anhui, China) Decoction Pieces Limited Company, and then authenticated by Dr. B. Liu (department of Botany of Beijing, University of Chinese Medicine). The drugs were extracted by the Chinese medicine preparation room of China-Japan Friendship Hospital as previously described [19 ]. The extraction rate was 18.8%, and the quality control of XYS was identified by high-performance liquid chromatography-mass spectrometry analysis (LC-MS/MS).
Composition of XYS
Medicinal plant | Amount (g) |
---|---|
Poria((Poria cocos (Schw.) Wolf)) | 15 |
Rhizoma Zingiberis Recens (Zingiber officinale Rosc.) | 15 |
Radix Angelicae Sinensis (Angelica sinensis (Oliv.) Diels) | 15 |
Rhizoma Atractylodis Macrocephalae (Atractylodes macrocephala Koidz.) | 15 |
Radix Paeoniae Alba (Paeonia lactiflora Pall.) | 15 |
Radix Glycyrrhizae (Glycyrrhiza uralensis Fisch.), | 6 |
Herba Menthae (Mentha haplocalyx Briq.) | 6 |
Radix Bupleuri (Bupleurum chinense DC.) | 15 |
Angelica sinensis
Atractylodes
Bupleurum chinense
Chinese
Glycyrrhiza uralensis
High-Performance Liquid Chromatographies
Mass Spectrometry
Medicinal Herbs
Mentha
Paeonia
Pharmaceutical Preparations
Poria
Wolfiporia extensa
Wolves
Zingiber officinale
Xiaoyaosan decoction consists of 300 g of Poria cocos (Schw.) Wolf (Poria), 300 g of Paeonia lactiflora Pall. (Radix Paeoniae Alba), 150 g of Glycyrrhiza uralensis Fisch. (Radix Glycyrrhizae), 300 g of Bupleurum chinense DC. (Radix Bupleuri), 300 g of Angelica sinensis (Oliv.) Diels (Radix Angelicae Sinensis), 300 g of Atractylodes macrocephala Koidz. (Rhizoma Atractylodis Macrocephalae), 100 g of Mentha haplocalyx Briq. (Herba Menthae), and 100 g of Zingiber officinale Rosc. (Rhizoma Zingiberis Recens). These eight herbs were purchased from Beijing Tongrentang Co., Ltd. The 8 herbs were processed into dry extract in the Chinese medicine preparation room of the China-Japan Friendship Hospital (Beijing), following the Regulation on Processing of Traditional Chinese Medical Herbal Pieces of Beijing. All raw materials were extracted by boiling water three times, and then the decoction was dehydrated in vacuo (70°C) and ground into powder for use. The extraction rate of the dry extract was 18.8%, dosage of Xiaoyaosan = 6.17 × crud herbs ÷ 60 kg (normal human body weight) × extraction rate (actual dry powder/actual crude herbs). Xiaoyaosan dissolved in deionized water was gavaged at a dose of 3.854 g/Kg·d [19 ], one time per day, 1 mL/100 g bodyweight. 20 mg/capsule of fluoxetine dissolved in deionized water was gavaged based on body weight. Group N, group T, and group T+D were gavaged with deionized water.
Angelica sinensis
Atractylodes
Body Weight
Bupleurum chinense
Bupleurum root
Capsule
Chinese
Fluoxetine
Glycyrrhiza uralensis
Mentha
Paeonia
Pharmaceutical Preparations
Plant Roots
Poria
Powder
rhizoma zingiberis recens
Rhizome
Wolfiporia extensa
Wolves
xiaoyaosan
Zingiber officinale
Most recents protocols related to «Glycyrrhiza uralensis»
SLBZS is composed of Panax Ginseng, Wolfiporia cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandiflorus and Glycyrrhiza uralensis Fisch, all herbs were purchased from Beijing Tongrentang Guangzhou pharmaceutical chain Co., Ltd (Guangzhou, China). Panax Ginseng, Wolfiporia cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandiflorus and Glycyrrhiza uralensis Fisch at a ratio of 4:4:4:4:3:2:2:2:2:4, pulverize in a beater and pass through a sieve of 60 mesh (19 (link), 20 (link), 22 (link)). 10g SLBZS powder was accurately weighed in a 250 ml conical flask, and 4% (corn flour by weight/substrate by weight) of corn flour was added to it. High-temperature sterilization was performed at 121°C for 20 min, and cooling to room temperature for later use.
Atractylodes
Balloon Flower
Corn Flour
Dioscorea opposita
Dolichos
Fever
Fruit
Glycyrrhiza uralensis
Panax ginseng
Pharmaceutical Preparations
Plant Embryos
Powder
Sterilization
Wolfiporia extensa
Eucommia ulmoides Oliv. and Glycyrrhiza uralensis Fisch. were purchased from Zhejiang Chinese Medical University Chinese Herbal Pieces Co., Ltd. (Hangzhou, China) (lot. No. 210201). The identification of the two herbs used in this study was undertaken by Zhejiang Chinese Medical University Chinese Herbal Pieces Co., Ltd. on the basis of the Chinese Pharmacopeia (2020, Edition). According to the ratio in the prescription, the drug was finally prepared into 630 g/L and stored in aliquots at −20 °C until use. For untargeted metabolomics analysis of E.G., the extraction solution was diluted with methanol to 10 mg/mL and centrifuged (4 °C, 12,000 g, and 10 min) to obtain the supernatant. The supernatant was filtered through a microporous membrane filter of 0.22 µm in diameter. Prior to analysis, the sample was stored at 4 °C.
Chinese
Eucommia ulmoides
Glycyrrhiza uralensis
Methanol
Pharmaceutical Preparations
Tissue, Membrane
Glycyrrhizae Radix et Rhizoma was purchased from Kangmei Pharmaceutical Co. Ltd. (Guangzhou, China) and authenticated by Professor Zhi Chao (Southern Medical University) as the dried root and rhizome of Glycyrrhiza uralensis Fisch. Enzyme-linked immunosorbent assay (ELISA) kits for tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were obtained from Cusabio (Wuhan, China). PrimeScriptTM RT Master Mix and TB GreenTM Premix Ex TaqTM II were from Takara (Shiga, Japan). Bestar R qPCR Master Mix was obtained from DBI Bioscience (Shanghai, China). Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against dengue virus E protein and NS1 protein were purchased from GeneTex (San Antonio, TX, USA) and Arigo Biolaboratories (Taiwan, China), respectively. The antibody against β-actin was provided by Santa Cruz (Santa Cruz, CA, USA). Anti-mouse IgG HRP-linked antibody and Anti-rabbit IgG HRP-linked antibody were obtained from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor 488-conjugated anti-Rabbit IgG antibody, Alexa Fluor 555-conjugated anti-Mouse IgG antibody, and Lipofectamine® 2000 transfection reagent were purchased from Invitrogen (Grand Island, NE, USA). 4′, 6-diamidino-2-phenylindole (DAPI) was obtained from Bioss (Beijing, China). RPMI-1640, DMEM, penicillin, streptomycin, the BCA protein assay kit, and the enhanced chemiluminescence (ECL) kit were purchased from Thermo Fisher Scientific. Methylcellulose, crystal violet, and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). ED III protein was provided by China Peptides Co., Ltd.
Actins
alexa fluor 488
Alexa Fluor 555
Antibodies
Antibodies, Anti-Idiotypic
Biological Assay
Chemiluminescence
Dengue Fever
Dengue Virus
Enzyme-Linked Immunosorbent Assay
Fetal Bovine Serum
Glycyrrhiza uralensis
IGG-horseradish peroxidase
Immunoglobulin G
Immunoglobulins
Interleukin-6
lipofectamine 2000
Methylcellulose
Mus
Penicillins
Peptides
Pharmaceutical Preparations
Plant Roots
Proteins
Rabbits
Rhizome
Streptomycin
Transfection
Tumor Necrosis Factor-alpha
Violet, Gentian
Viral Proteins
Compound chemical composition and drug targets were collected and predicted. Dangshen (Codonopsis pilosula (Franch.) Nannf), Huangqin (Scutellaria baicalensis Georgi), Baizhu (Atractylodes macrocephala Koidz), Fuling (Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb), Huangqi (Astragalus membranaceus (Fisch.) Bunge), Yiyiren (Semen Coicis), Wumei (Fructus Mume), Fangfeng (Saposhnikovia divaricata (Turcz.) Schischk), Chenpi (Citrus reticulata Blanco), Niuxi (Achyranthes bidentata Blume), and Gancao (Glycyrrhiza uralensis Fisch) were inputted to the TCMSP database (https://old.tcmsp-e.com/tcmsp.php ), to collect all compounds [12 (link)]. Then, according to the pharmacokinetic principle, the suitable compounds are screened based on oral bioavailability (OB) ≥ 30% and drug-like index (DL) ≥ 0.18. Drug target data were obtained from DrugBank (https://go.drugbank.com/ ) and standardized using the UniProt (https://www.Uniprot.org/ ) database [13 (link)]. Based on GeneCards (https://www.genecards.org/ ) and OMIM-NCBI databases (https://www.ncbi.nlm.nih.gov/omim ), IBD as keyword was used to screen disease targets, while for species selection “Homosapiens” was used. Draw Venn Diagrams tool in R software is used to process drug targets and disease targets obtained in the above steps to obtain the intersection gene targets and output Venn Diagram for display.
PPI network interaction analysis was performed on the active chemical components and core targets of Qinghua Jianpi Recipe by using the STRING database and Cytoscape3.7.1 software. According to the requirements of Cytoscape, the “source-target” data table was constructed, and the NetworkAnalyzer plug-in was used to construct the regulation network of TCM, active component, and target. In the generated regulation network, nodes represent the interaction between TCM, active component, and target, and edges represent the interaction between active the component and target disease. Furthermore, MCC algorithm of CytoHubba plug-in was used to calculate and construct PPI network of each target. Based on Bioconductor (https://www.bioconductor.org/ ) in the R package (https://www.r-project.org/ ) and clusterProfiler 3.12.0 to GO (gene ontology) core target function and KEGG pathway enrichment analysis (KEGG pathway analysis), Homo sapiens was selected and a threshold P < 0.05 was set. According to the results, the core target-critical pathway network was conducted in Cytoscape 3.7.1 software.
PPI network interaction analysis was performed on the active chemical components and core targets of Qinghua Jianpi Recipe by using the STRING database and Cytoscape3.7.1 software. According to the requirements of Cytoscape, the “source-target” data table was constructed, and the NetworkAnalyzer plug-in was used to construct the regulation network of TCM, active component, and target. In the generated regulation network, nodes represent the interaction between TCM, active component, and target, and edges represent the interaction between active the component and target disease. Furthermore, MCC algorithm of CytoHubba plug-in was used to calculate and construct PPI network of each target. Based on Bioconductor (
Achyranthes
Astragalus membranaceus
Atractylodes
Baizhu
chemical composition
chenpi
Citrus reticulata
Codonopsis
Dang Shen
Drug Delivery Systems
Fruit
Genes
glycyrrhizae radix et rhizoma
Glycyrrhiza uralensis
Homo sapiens
Huang Qi
Huangqin
Pharmaceutical Preparations
Plant Embryos
Saposhnikovia
Wolfiporia extensa
Wolves
wumei
Lingguizhugan decoction (powder batch number: Z201101) was provided by Professor Tong Zhang, School of Pharmacy, Shanghai University of Traditional Chinese Medicine. Poriacocos (Schw.) Wolf (batch number: Y2003002), Cinnamomum cassia Presl (batch number: 200608), Atractylodes macrocephala Koidz. (batch number: YP200601), and Glycyrrhiza uralensis Fisch. (batch number: YP200601) in a 2:1.5:1:1 ratio, added 12 times the amount of water, decoction 2 times, every 1.5 hours, collected the first water decoction, and set aside. Decoction filtered, combined two filtrates, the filtrate concentrated to a relative density of 1.07 ~ 1.09 (65 ± 5°C), spray-dried, and crushed into a fine powder for use (4.56 g crude medicine extracted 1 g extract powder, which was stored in a dry environment). All herbs were purchased from Jiangsu Sanhexing Chinese Medicine Research Co., Ltd. (Jiangsu, China). The fingerprint was used to control the quality of the LGZG (Supplementary Figure 1 ).
Atractylodes
Chinese
Cinnamomum cassia
Glycyrrhiza uralensis
Pharmaceutical Preparations
Powder
Wolves
Top products related to «Glycyrrhiza uralensis»
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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2,4,6-trinitrobenzenesulfonic acid (TNBS) is a laboratory reagent commonly used in analytical and biochemical applications. It is a crystalline solid with a chemical formula C6H3N3O9S. TNBS is primarily used as a derivatizing agent for the detection and quantification of primary amines. Its core function is to facilitate the spectrophotometric determination of amino groups in various biomolecules and chemical compounds.
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Formic acid is a clear, colorless liquid chemical compound used in various industrial and laboratory applications. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid has a pungent odor and is highly corrosive. It is commonly used as a preservative, pH adjuster, and analytical reagent in laboratory settings.
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HPLC-grade acetonitrile is a high-purity organic solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the specifications required for HPLC-grade solvents, ensuring consistency and reliability in analytical procedures.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
Sourced in China
Liquiritin is a chemical compound extracted from the roots of the licorice plant (Glycyrrhiza glabra). It is a key component in various pharmaceutical and cosmetic products. Liquiritin serves as a natural flavoring agent and has demonstrated anti-inflammatory properties. The specific details and intended uses of this product are not within the scope of this response.
More about "Glycyrrhiza uralensis"
Glycyrrhiza uralensis, also known as the Chinese licorice, is a perennial herb native to Central Asia.
This medicinal plant has a long history of use in traditional Chinese medicine, where its roots are valued for their various therapeutic properties.
Glycyrrhiza uralensis has gained significant attention in the scientific community due to its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects.
Reasearch into Glycyrrhiza uralensis is crucial for understanding its pharmacological potential and developing new therapeutic applications.
Key areas of study include the extraction and isolation of bioactive compounds like glycyrrhizin, liquiritin, and formic acid, as well as the use of solvents like DMSO, acetonitrile, and methanol in analytical techniques such as HPLC.
PubCompare.ai's revolutionary AI-driven protocol comparison tool can help streamline Glycyrrhiza uralensis research by enhancing reproducibility and accuracy.
This innovative platform allows researchers to easily identify the best protocols and products from literature, preprints, and patents, accelerating the discovery of new and effective applications for this remarkable medicinal plant.
Utilizing PubCompare.ai can take your Glycyrrhiza uralensis studies to the next level by providing access to a wealth of data and insights.
Explore the latest research, optimize your experimental design, and collaborate more effectively with colleagues to advance our understanding of this fascinating herb and its potential therapeutic uses.
This medicinal plant has a long history of use in traditional Chinese medicine, where its roots are valued for their various therapeutic properties.
Glycyrrhiza uralensis has gained significant attention in the scientific community due to its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects.
Reasearch into Glycyrrhiza uralensis is crucial for understanding its pharmacological potential and developing new therapeutic applications.
Key areas of study include the extraction and isolation of bioactive compounds like glycyrrhizin, liquiritin, and formic acid, as well as the use of solvents like DMSO, acetonitrile, and methanol in analytical techniques such as HPLC.
PubCompare.ai's revolutionary AI-driven protocol comparison tool can help streamline Glycyrrhiza uralensis research by enhancing reproducibility and accuracy.
This innovative platform allows researchers to easily identify the best protocols and products from literature, preprints, and patents, accelerating the discovery of new and effective applications for this remarkable medicinal plant.
Utilizing PubCompare.ai can take your Glycyrrhiza uralensis studies to the next level by providing access to a wealth of data and insights.
Explore the latest research, optimize your experimental design, and collaborate more effectively with colleagues to advance our understanding of this fascinating herb and its potential therapeutic uses.