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Glycyrrhiza uralensis

Glycyrrhiza uralensis, also known as the Chinese licorice, is a perennial herb native to Central Asia.
It has a long history of use in traditional Chinese medicine, where its roots are valued for their various therapeutic properties.
This plant has gained significant attention in the scientific community due to its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects.
Reserach into Glycyrrhiza uralensis is crucial for understanding its pharmacological potential and developing new therapeutic applications.
PubCompare.ai's revolutionary AI-driven protocol comparison tool can help streamline this research by enhacing reproducibility and accuracy, allowing researchers to easily identify the best protocols and products from literature, preprints, and patents.
Utilizing this innovative platform can take your Glycyrrhiza uralensis studies to the next level and accelerate the discovery of new and effective applications for this remarkable medicinal plant.

Most cited protocols related to «Glycyrrhiza uralensis»


Glycyrrhiza uralensis was used as an example to introduce the comparative genomic approach to predict the GO annotation. We use BLAST-2.2.19 (-e 1e-3 -m 8) to compare the protein sequences of G. uralensis (34 (link)) and Arabidopsis thaliana (35 (link)). Then, the orthologous pairs with A. thaliana were determined, as was the corresponding GO annotation. Additionally, Blast2GO has a comprehensive bioinformatics platform that can automatically predict GO annotation based on the sequence information and a powerful remote annotation background (36 (link)). After the BLAST, interpro and mapping analyses steps in the Blast2GO application, we can export the GO annotation file and update agriGO v2.0. The InterPro (37 (link)) ID and Pfam (38 (link)) accessions have internal connections with the GO. Thus, we can map the GO terms to candidate genes using information on specific domains.
Publication 2017
Amino Acid Sequence Arabidopsis thalianas Base Sequence FCER2 protein, human Genes Glycyrrhiza uralensis
We also generated a HPLC fingerprint of ASHMI™ as well as the individual herbs in ASHMI™ as a means of standardization according to FDA’s guidance for industrial botanical drug products.13 9.4 mg of Gan-Cao, 20.9 mg of Ku-Shen, 16.4 mg of Ling-Zhi, and 50 mg of ASHMI™ extracts were dissolved in 10 ml of H2O. The solution was transferred to a separatory funnel, and extracted with 5 ml of n-butanol three times. The combined extracts were dried and dissolved in 50% aqueous methanol. The solution was then transferred to a 2 ml volumetric flask. The resulting solution was filtered through 0.2 µm filter (Whatman Inc.,Sanford, ME, USA). 10 µL solution was then analyzed by using Waters Alliance 2695 HPLC system with photodiode array detector (2996) (Waters Corporation , Milford, MA, USA)]. Data were acquired and processed with Waters’ Empower software. The separation was achieved on Zorbax SB-C18 column (150 × 4.6 mm; 5µm particle size) from Agilent Technologies (Santa Clara, CA, USA) and a Agilent’s Zorbax C18 Guard column was also installed on it. The mobile phases include 0.15% H3PO4 (A) and acetonitrile (B).The separation gradient started at 2% of B to 48 % for 75 min and further increased to 70% in 4 min. The total chromatographic run time was 80 min. The flow rate was adjusted to1.0 mL/min and the column temperature was set to be 27°C. All the chemicals and solvent used are HPLC grade from Fisher Scientific (Fisher Scientific, Pittsburgh, PA, USA). Figure 2 shows the 2D-HPLC profiles (at 254 nm) of each herb in parallel with that of the entire ASHMI™ formula. By comparing the on-line ultraviolet (UV) spectra and retention time (tR), most diagnostic peaks in the fingerprint of ASHMI™ were correlated with individual herbal medicines. Peaks 16, 21 and 32 originated from Gan-Cao, peaks 12, 13, 14, 18, 19, 20 and 22 from Ku-Shen, and peaks 23, 24, 27, 28, 29, 31, 32, 33 from Ling-Zhi. Two batches of ASHMI™ were used in the study; HPLC was similar for both batches.
Publication 2009
acetonitrile Butyl Alcohol Chromatography Diagnosis Glycyrrhiza uralensis High-Performance Liquid Chromatographies Medicines, Herbal Methanol Retention (Psychology) Solvents
A total of 18 batches of HQT were included in the present study. Four batches coded as PHY906-6, 7, 8, 10 were manufactured with PhytoCeutica's proprietary SOP. Eight batches of HQT were purchased from Sun Ten Pharmaceutical Co. LTD in Taiwan and designated as HQT-E, F, G, H, I, J, K and L. Six batches of HQT were obtained from various vendors (Chung Song Zong, Ko Da, Min Tong, Sheng Chang, Sheng Foong, Kaiser; Taiwan) who did not provide quality information, and were labeled as HQT-CSZ, KD, MT, SC, SF and KP3. The proprietary standard operating procedures (SOP) by PhytoCeutica for PHY906 used hot water extraction (80°C) of four herbs, namely Scutellaria baicalensis Georgi (S), Paeonia lactiflora Pall. (P), Glycyrrhiza uralensis Fisch. (G) and Ziziphus jujuba Mill. (Z) (ratio 3:2:2:2). The hot water extraction is then spray dried with insoluble dextran into a granulated powder, packaged and stored in foil containers at 4°C.
Chemical standards including baicalin (S), baicalein (S), wogonin (S), scutellarin (S), glycyrrhizin (G), ononin (G), liquiritin (G), liqiritigenin (G), paeoniflorin (P) and albiflorin (P), were obtained from Chromadex (USA). Apigenin and formic acid were obtained from Sigma-Aldrich (USA). Solvents were of LC/MS grade from JT Baker (USA).
Publication 2010
albiflorin Apigenin baicalein baicalin Dextran formic acid Glycyrrhiza uralensis Glycyrrhizic Acid Huangqin liquiritin ononin Paeonia peoniflorin Pharmaceutical Preparations PHY 906 Powder scutellarin Solvents wogonin Ziziphus
The XYS formula was composed of eight herbal medicines. The composition and dose of the prescription is listed in Table 1. The raw herbs were obtained from the Tongrentang (Bozhou, Anhui, China) Decoction Pieces Limited Company, and then authenticated by Dr. B. Liu (department of Botany of Beijing, University of Chinese Medicine). The drugs were extracted by the Chinese medicine preparation room of China-Japan Friendship Hospital as previously described [19 ]. The extraction rate was 18.8%, and the quality control of XYS was identified by high-performance liquid chromatography-mass spectrometry analysis (LC-MS/MS).

Composition of XYS

Medicinal plantAmount (g)
Poria((Poria cocos (Schw.) Wolf))15
Rhizoma Zingiberis Recens (Zingiber officinale Rosc.)15
Radix Angelicae Sinensis (Angelica sinensis (Oliv.) Diels)15
Rhizoma Atractylodis Macrocephalae (Atractylodes macrocephala Koidz.)15
Radix Paeoniae Alba (Paeonia lactiflora Pall.)15
Radix Glycyrrhizae (Glycyrrhiza uralensis Fisch.),6
Herba Menthae (Mentha haplocalyx Briq.)6
Radix Bupleuri (Bupleurum chinense DC.)15
Publication 2017
Angelica sinensis Atractylodes Bupleurum chinense Chinese Glycyrrhiza uralensis High-Performance Liquid Chromatographies Mass Spectrometry Medicinal Herbs Mentha Paeonia Pharmaceutical Preparations Poria Wolfiporia extensa Wolves Zingiber officinale
Xiaoyaosan decoction consists of 300 g of Poria cocos (Schw.) Wolf (Poria), 300 g of Paeonia lactiflora Pall. (Radix Paeoniae Alba), 150 g of Glycyrrhiza uralensis Fisch. (Radix Glycyrrhizae), 300 g of Bupleurum chinense DC. (Radix Bupleuri), 300 g of Angelica sinensis (Oliv.) Diels (Radix Angelicae Sinensis), 300 g of Atractylodes macrocephala Koidz. (Rhizoma Atractylodis Macrocephalae), 100 g of Mentha haplocalyx Briq. (Herba Menthae), and 100 g of Zingiber officinale Rosc. (Rhizoma Zingiberis Recens). These eight herbs were purchased from Beijing Tongrentang Co., Ltd. The 8 herbs were processed into dry extract in the Chinese medicine preparation room of the China-Japan Friendship Hospital (Beijing), following the Regulation on Processing of Traditional Chinese Medical Herbal Pieces of Beijing. All raw materials were extracted by boiling water three times, and then the decoction was dehydrated in vacuo (70°C) and ground into powder for use. The extraction rate of the dry extract was 18.8%, dosage of Xiaoyaosan = 6.17 × crud herbs ÷ 60 kg (normal human body weight) × extraction rate (actual dry powder/actual crude herbs). Xiaoyaosan dissolved in deionized water was gavaged at a dose of 3.854 g/Kg·d [19 ], one time per day, 1 mL/100 g bodyweight. 20 mg/capsule of fluoxetine dissolved in deionized water was gavaged based on body weight. Group N, group T, and group T+D were gavaged with deionized water.
Publication 2015
Angelica sinensis Atractylodes Body Weight Bupleurum chinense Bupleurum root Capsule Chinese Fluoxetine Glycyrrhiza uralensis Mentha Paeonia Pharmaceutical Preparations Plant Roots Poria Powder rhizoma zingiberis recens Rhizome Wolfiporia extensa Wolves xiaoyaosan Zingiber officinale

Most recents protocols related to «Glycyrrhiza uralensis»

SLBZS is composed of Panax Ginseng, Wolfiporia cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandiflorus and Glycyrrhiza uralensis Fisch, all herbs were purchased from Beijing Tongrentang Guangzhou pharmaceutical chain Co., Ltd (Guangzhou, China). Panax Ginseng, Wolfiporia cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandiflorus and Glycyrrhiza uralensis Fisch at a ratio of 4:4:4:4:3:2:2:2:2:4, pulverize in a beater and pass through a sieve of 60 mesh (19 (link), 20 (link), 22 (link)). 10g SLBZS powder was accurately weighed in a 250 ml conical flask, and 4% (corn flour by weight/substrate by weight) of corn flour was added to it. High-temperature sterilization was performed at 121°C for 20 min, and cooling to room temperature for later use.
Publication 2023
Atractylodes Balloon Flower Corn Flour Dioscorea opposita Dolichos Fever Fruit Glycyrrhiza uralensis Panax ginseng Pharmaceutical Preparations Plant Embryos Powder Sterilization Wolfiporia extensa
Eucommia ulmoides Oliv. and Glycyrrhiza uralensis Fisch. were purchased from Zhejiang Chinese Medical University Chinese Herbal Pieces Co., Ltd. (Hangzhou, China) (lot. No. 210201). The identification of the two herbs used in this study was undertaken by Zhejiang Chinese Medical University Chinese Herbal Pieces Co., Ltd. on the basis of the Chinese Pharmacopeia (2020, Edition). According to the ratio in the prescription, the drug was finally prepared into 630 g/L and stored in aliquots at −20 °C until use. For untargeted metabolomics analysis of E.G., the extraction solution was diluted with methanol to 10 mg/mL and centrifuged (4 °C, 12,000 g, and 10 min) to obtain the supernatant. The supernatant was filtered through a microporous membrane filter of 0.22 µm in diameter. Prior to analysis, the sample was stored at 4 °C.
Publication 2023
Chinese Eucommia ulmoides Glycyrrhiza uralensis Methanol Pharmaceutical Preparations Tissue, Membrane
Glycyrrhizae Radix et Rhizoma was purchased from Kangmei Pharmaceutical Co. Ltd. (Guangzhou, China) and authenticated by Professor Zhi Chao (Southern Medical University) as the dried root and rhizome of Glycyrrhiza uralensis Fisch. Enzyme-linked immunosorbent assay (ELISA) kits for tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were obtained from Cusabio (Wuhan, China). PrimeScriptTM RT Master Mix and TB GreenTM Premix Ex TaqTM II were from Takara (Shiga, Japan). Bestar R qPCR Master Mix was obtained from DBI Bioscience (Shanghai, China). Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against dengue virus E protein and NS1 protein were purchased from GeneTex (San Antonio, TX, USA) and Arigo Biolaboratories (Taiwan, China), respectively. The antibody against β-actin was provided by Santa Cruz (Santa Cruz, CA, USA). Anti-mouse IgG HRP-linked antibody and Anti-rabbit IgG HRP-linked antibody were obtained from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor 488-conjugated anti-Rabbit IgG antibody, Alexa Fluor 555-conjugated anti-Mouse IgG antibody, and Lipofectamine® 2000 transfection reagent were purchased from Invitrogen (Grand Island, NE, USA). 4′, 6-diamidino-2-phenylindole (DAPI) was obtained from Bioss (Beijing, China). RPMI-1640, DMEM, penicillin, streptomycin, the BCA protein assay kit, and the enhanced chemiluminescence (ECL) kit were purchased from Thermo Fisher Scientific. Methylcellulose, crystal violet, and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). ED III protein was provided by China Peptides Co., Ltd.
Publication 2023
Actins alexa fluor 488 Alexa Fluor 555 Antibodies Antibodies, Anti-Idiotypic Biological Assay Chemiluminescence Dengue Fever Dengue Virus Enzyme-Linked Immunosorbent Assay Fetal Bovine Serum Glycyrrhiza uralensis IGG-horseradish peroxidase Immunoglobulin G Immunoglobulins Interleukin-6 lipofectamine 2000 Methylcellulose Mus Penicillins Peptides Pharmaceutical Preparations Plant Roots Proteins Rabbits Rhizome Streptomycin Transfection Tumor Necrosis Factor-alpha Violet, Gentian Viral Proteins
Compound chemical composition and drug targets were collected and predicted. Dangshen (Codonopsis pilosula (Franch.) Nannf), Huangqin (Scutellaria baicalensis Georgi), Baizhu (Atractylodes macrocephala Koidz), Fuling (Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb), Huangqi (Astragalus membranaceus (Fisch.) Bunge), Yiyiren (Semen Coicis), Wumei (Fructus Mume), Fangfeng (Saposhnikovia divaricata (Turcz.) Schischk), Chenpi (Citrus reticulata Blanco), Niuxi (Achyranthes bidentata Blume), and Gancao (Glycyrrhiza uralensis Fisch) were inputted to the TCMSP database (https://old.tcmsp-e.com/tcmsp.php), to collect all compounds [12 (link)]. Then, according to the pharmacokinetic principle, the suitable compounds are screened based on oral bioavailability (OB) ≥ 30% and drug-like index (DL) ≥ 0.18. Drug target data were obtained from DrugBank (https://go.drugbank.com/) and standardized using the UniProt (https://www.Uniprot.org/) database [13 (link)]. Based on GeneCards (https://www.genecards.org/) and OMIM-NCBI databases (https://www.ncbi.nlm.nih.gov/omim), IBD as keyword was used to screen disease targets, while for species selection “Homosapiens” was used. Draw Venn Diagrams tool in R software is used to process drug targets and disease targets obtained in the above steps to obtain the intersection gene targets and output Venn Diagram for display.
PPI network interaction analysis was performed on the active chemical components and core targets of Qinghua Jianpi Recipe by using the STRING database and Cytoscape3.7.1 software. According to the requirements of Cytoscape, the “source-target” data table was constructed, and the NetworkAnalyzer plug-in was used to construct the regulation network of TCM, active component, and target. In the generated regulation network, nodes represent the interaction between TCM, active component, and target, and edges represent the interaction between active the component and target disease. Furthermore, MCC algorithm of CytoHubba plug-in was used to calculate and construct PPI network of each target. Based on Bioconductor (https://www.bioconductor.org/) in the R package (https://www.r-project.org/) and clusterProfiler 3.12.0 to GO (gene ontology) core target function and KEGG pathway enrichment analysis (KEGG pathway analysis), Homo sapiens was selected and a threshold P < 0.05 was set. According to the results, the core target-critical pathway network was conducted in Cytoscape 3.7.1 software.
Publication 2023
Achyranthes Astragalus membranaceus Atractylodes Baizhu chemical composition chenpi Citrus reticulata Codonopsis Dang Shen Drug Delivery Systems Fruit Genes glycyrrhizae radix et rhizoma Glycyrrhiza uralensis Homo sapiens Huang Qi Huangqin Pharmaceutical Preparations Plant Embryos Saposhnikovia Wolfiporia extensa Wolves wumei
Lingguizhugan decoction (powder batch number: Z201101) was provided by Professor Tong Zhang, School of Pharmacy, Shanghai University of Traditional Chinese Medicine. Poriacocos (Schw.) Wolf (batch number: Y2003002), Cinnamomum cassia Presl (batch number: 200608), Atractylodes macrocephala Koidz. (batch number: YP200601), and Glycyrrhiza uralensis Fisch. (batch number: YP200601) in a 2:1.5:1:1 ratio, added 12 times the amount of water, decoction 2 times, every 1.5 hours, collected the first water decoction, and set aside. Decoction filtered, combined two filtrates, the filtrate concentrated to a relative density of 1.07 ~ 1.09 (65 ± 5°C), spray-dried, and crushed into a fine powder for use (4.56 g crude medicine extracted 1 g extract powder, which was stored in a dry environment). All herbs were purchased from Jiangsu Sanhexing Chinese Medicine Research Co., Ltd. (Jiangsu, China). The fingerprint was used to control the quality of the LGZG (Supplementary Figure 1).
Publication 2023
Atractylodes Chinese Cinnamomum cassia Glycyrrhiza uralensis Pharmaceutical Preparations Powder Wolves

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HPLC-grade acetonitrile is a high-purity organic solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. The product meets the specifications required for HPLC-grade solvents, ensuring consistency and reliability in analytical procedures.
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Liquiritin is a chemical compound extracted from the roots of the licorice plant (Glycyrrhiza glabra). It is a key component in various pharmaceutical and cosmetic products. Liquiritin serves as a natural flavoring agent and has demonstrated anti-inflammatory properties. The specific details and intended uses of this product are not within the scope of this response.

More about "Glycyrrhiza uralensis"

Glycyrrhiza uralensis, also known as the Chinese licorice, is a perennial herb native to Central Asia.
This medicinal plant has a long history of use in traditional Chinese medicine, where its roots are valued for their various therapeutic properties.
Glycyrrhiza uralensis has gained significant attention in the scientific community due to its potential health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects.
Reasearch into Glycyrrhiza uralensis is crucial for understanding its pharmacological potential and developing new therapeutic applications.
Key areas of study include the extraction and isolation of bioactive compounds like glycyrrhizin, liquiritin, and formic acid, as well as the use of solvents like DMSO, acetonitrile, and methanol in analytical techniques such as HPLC.
PubCompare.ai's revolutionary AI-driven protocol comparison tool can help streamline Glycyrrhiza uralensis research by enhancing reproducibility and accuracy.
This innovative platform allows researchers to easily identify the best protocols and products from literature, preprints, and patents, accelerating the discovery of new and effective applications for this remarkable medicinal plant.
Utilizing PubCompare.ai can take your Glycyrrhiza uralensis studies to the next level by providing access to a wealth of data and insights.
Explore the latest research, optimize your experimental design, and collaborate more effectively with colleagues to advance our understanding of this fascinating herb and its potential therapeutic uses.