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Gossypium

Gossypium is a genus of plants in the family Malvaceae, commonly known as cotton.
These perennial shrubs or small trees are native to tropical and subtropical regions, and are cultivated worldwide for their valuable fiber, which is the primary source of natural textile fibers.
Gossypium species display a diverse range of morphological characteristics, including variation in leaf shape, flower color, and boll (fruit) size and structure.
Understanding the biology and taxonomy of Gossypium is crucial for improving cotton crop yield and quality, as well as developing new textile applications.
Researchers can leverage the power of PubCompare.ai to streamline their Gossypium research, easily locating relevant protocols and identifying the most accurate and reproducible methods to advance their findings.

Most cited protocols related to «Gossypium»

1
Vaginal Cytology for Mouse Estrous Cycle
Cited 110 times
A vaginal swab was collected using a cotton tipped swab (Puritan Medical Products Company, LLC Guilford, ME) wetted with ambient temperature physiological saline and inserted into the vagina of the restrained mouse. The swab was gently turned and rolled against the vaginal wall and then removed. Cells were transferred to a dry glass slide by rolling the swab across the slide. The slide was air dried and then stained with approximately 400 µL of stain (Accustain, Sigma-Aldrich, St. Louis, MO) for 45 seconds. The slides were rinsed with water, overlaid with a coverslip, and viewed immediately at 200× magnification under bright field illumination. The stage of the estrous cycle was determined based on the presence or absence of leukocytes, cornified epithelial, and nucleated epithelial cells according to Felicio, et al [9] (link).
When the female is in proestrus, mostly nucleated and some cornified epithelial cells are present. Some leukocytes may be present if the female is in early proestrus. As the stage of the cycle advances to estrus, mostly cornified epithelial cells are present. If the cycle is not interrupted by pregnancy, pseudopregnancy, or other phenomena, metestrus will begin. Metestrus is a brief stage when the corpora lutea form but fail to fully luteinize due to a lack of progesterone. The uterine lining will begin to slough and evidence of this is seen in the form of cornified eipithelial cells and polymorphonuclear leukocytes present in vaginal swabs. Some nucleated epithelia cells will also be present in late metestrus. Diestrus is the longest of the stages lasting more than 2 days. Vaginal swabs during diestrus show primarily polymorphonuclear leukocytes and a few epithelial cells during late diestrus. Leukocytes remain the predominant cell type having removed cellular debris. The cycle then repeats.
Byers S.L., Wiles M.V., Dunn S.L, & Taft R.A. (2012). Mouse Estrous Cycle Identification Tool and Images. PLoS ONE, 7(4), e35538.
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Publication 2012
Cells Corpus Luteum Diestrus Epithelial Cells Epithelioid Cells Estrous Cycle Estrus Gossypium Granulocyte Leukocytes Lighting Metestrus Mus Neoplasm Metastasis physiology Pregnancy Proestrus Progesterone Pseudocyesis Saline Solution Stains Uterus Vagina Vision Woman
2
Two-Stage Sulfuric Acid Hydrolysis for Lignin Analysis
Cited 74 times
The use of a two-stage sulfuric acid hydrolysis for the analysis of lignin dates to the turn of the 20th century, although the use of concentrated acid to release sugars from wood dates to the early 19th century (7 ). Klason, in 1906, is often credited as the first to use sulfuric acid to isolate lignin from wood (7 −9 ). The method became named after Klason, and the insoluble residue from the test is known as “Klason lignin.” An English translation of a Klason paper, from this period (10 ), describes his attempt to determine the structure of spruce wood lignin. According to Brauns (7 ), Klason’s method originally used 72 wt % sulfuric acid; he later reduced this to 66 wt % to gelatinize the wood. He filtered the solids and subjected them to a second hydrolysis in 0.5 wt % hydrochloric acid.
Although Klason is generally credited as being the first to use sulfuric acid for lignin analysis, Sherrard and Harris (11 ) credit the use of sulfuric acid to Fleschsig in 1883, Ost and Wilkening in 1912, and König and Rump in 1913. According to Harris (12 ), Fleschsig, in 1883, dissolved cotton cellulose and converted it nearly quantitatively into sugars using strong sulfuric acid followed by dilution and heating. According to Browning (13 ), Ost and Wilkening introduced the use of 72 wt % sulfuric acid for lignin determinations in 1910. A translated paper by Heuser (14 ) credited König and Ost and Wilkening for the sulfuric acid lignin method. Dore (15 ) described several improved analytical methods (cellulose, lignin, soluble pentosans, mannan, and galactan) for the summative analysis of coniferous woods. The discrepancies in attribution may be due to differing definitions for the method cited (e.g., first to use acid to determine lignin, first to use sulfuric acid, first to use 72 wt % sulfuric acid, etc.) and to missed citations across continental distances in the early 20th century.
Sluiter J.B., Ruiz R.O., Scarlata C.J., Sluiter A.D, & Templeton D.W. (2010). Compositional Analysis of Lignocellulosic Feedstocks. 1. Review and Description of Methods. Journal of Agricultural and Food Chemistry, 58(16), 9043-9053.
Publication 2010
Acids Cellulose Galactans Gossypium Hydrochloric acid Hydrolysis Lignin Mannans Pentosan Sulfuric Polyester Picea Sugars sulfuric acid Technique, Dilution Tracheophyta Xylose
3
Rearing Anopheles funestus Mosquitoes
Cited 55 times
Blood fed An. funestus adult females resting indoor were collected in houses between 06 and 12 AM in Tororo District (0°45′ N, 34°5′E) in Eastern Uganda, an area of high malaria transmission [2] (link). The collection was carried out in April and November 2009. Blood-fed and gravid mosquitoes resting inside houses were collected using aspirators and torches and immediately transported to the laboratory of the National Livestock Resources Research Institute based in Tororo. A new method was used to induce the females to lay eggs. Briefly, the collected blood fed females were stored in net covered paper cups or in cages and provided with cotton wool moistened with sucrose. They were maintained for 4 to 5 days to allow them to fully reach the gravid stage and were checked daily for survival. The gravid mosquitoes were then gently individually introduced into 1.5 ml Eppendorf tubes containing a 1square cm filter paper inserted into the bottom of the tube. The filter paper was moistened and excess water removed. The cap of the Eppendorf tube was pierced with 3 holes to allow air into the tube. The tubes were checked daily for the presence of eggs. Females that laid eggs were carefully removed from the tubes and transferred into Eppendorf tubes with silica gel. Eggs were stored at room temperature or at 4°C for up to 2 days and then sent via courier to the Liverpool School of Tropical Medicine (LSTM) where they were allowed to hatch in small cup and later transferred to larvae bowls for rearing. Apart from 20 families that were reared individually, the egg batches were pooled and reared together. Larvae were comparatively reared in mineral (bottled) and distilled water and fed abundantly with TetraminTM baby fish food every day. Water of each larvae bowl was changed every two days to reduce the mortality. The F1 adults generated were randomly mixed in cages for subsequent experiments.
Morgan J.C., Irving H., Okedi L.M., Steven A, & Wondji C.S. (2010). Pyrethroid Resistance in an Anopheles funestus Population from Uganda. PLoS ONE, 5(7), e11872.
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Publication 2010
Adult BLOOD Culicidae Females Fishes Gossypium Infant Food Larva Livestock Malaria Minerals Silica Gel Sucrose Transmission, Communicable Disease Woman
4
Socioeconomic Status, Race, and Diurnal Cortisol
Cited 51 times

Protocol full text hidden due to copyright restrictions

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Publication 2010
1-palmitoyl-2-oleoylphosphatidylethanolamine Atherosclerosis Biological Assay BLOOD Cardiovascular Diseases Disease Progression Emotions Epistropheus Ethnicity Family Member Fingers Gender Gossypium Head Health Planning Heart Hispanics Hormones Hostility Households Hydrocortisone Hypersensitivity Index, Body Mass Luminescent Measurements Lung Medical Devices Metabolic Equivalent Obesity Parent Saliva Sjogren's Syndrome Specimen Collection Steroids Trapezoid Bones Viscosity Woman
5
Nucleotide Extraction from Petri-dish Samples
Cited 44 times
For nucleotide extraction from the petri-dish sampler, a cotton swab was moistened with sterile water and used to wipe the surface. The tip of the swab was then cut directly into a tube containing lysing matrix E beads (MP Biomedicals, Burlingame, CA, USA). A volume of 400 μl each of Miller Phosphate Buffer and Miller SDS lysis buffer (Miller et al., 1999 (link)) and 450 μl of 25:24:1 phenol: chloroform: isoamyl alcohol were added. Tubes were homogenized using a Biospec Mini 8 bead mill (Biospec, Bartlesville, OK, USA) at full speed for 1 min. The supernatant, approximately 560 μl, was isolated after centrifuging at 10 000 g for 5 min at 4 °C and mixed with an equal volume of chloroform. The supernatant was again isolated, approximately 480 μl, after centrifuging at 10 000 g for 5 min at 4 °C, and then processed further with the MoBio Power Soil DNA Extraction Kit (Carlsbad, CA, USA), starting with two volumes of solution C4 to isolate DNA onto the spin filters. Genomic DNA was further cleaned with the C5 ethanol-based wash solution and finally eluted in 100 μl warm C6 elution buffer run twice through the filter. Negative controls of sterile cotton swabs were also processed, and any taxa detected were filtered out of the other samples.
Adams R.I., Miletto M., Taylor J.W, & Bruns T.D. (2013). Dispersal in microbes: fungi in indoor air are dominated by outdoor air and show dispersal limitation at short distances. The ISME Journal, 7(7), 1262-1273.
Publication 2013
Buffers Chloroform Ethanol Genome Gossypium Hyperostosis, Diffuse Idiopathic Skeletal isopentyl alcohol Nucleotides Phenol Phosphates Sterility, Reproductive

Most recents protocols related to «Gossypium»

1
Porous Materials for Charged Droplet Generation
Not available on PMC !

Example 3

Probe Materials

A number of porous materials were tested to generate charged droplets for mass spectrometry. The materials were shaped into triangles having sharp tips and sample solution was then applied to the constructed probes. Data herein show that any hydrophilic and porous substrate could be used successfully, including cotton swab, textile, plant tissues as well as different papers. The porous network or microchannels of these materials offered enough space to hold liquid and the hydrophilic environment made it possible for liquid transport by capillary action. Hydrophobic and porous substrates could also be used successfully with properly selected hydrophobic solvents.

For further investigation, six kinds of commercialized papers were selected and qualitatively tested to evaluate their capabilities in analyte detection. Filter papers and chromatography paper were made from cellulose, while glass microfiber filter paper was made from glass microfiber. FIG. 19 shows the mass spectra of cocaine detection on those papers. The spectrum of glass fiber paper (FIG. 19E) was unique because the intensity of background was two orders of magnitude lower than other papers and the cocaine peak (m/z, 304) could not be identified.

It was hypothesized that the glass fiber paper was working on mode II and prohibiting efficient droplet generation, due to the relative large thickness (˜2 mm). This hypothesis was proved by using a thin layer peeled from glass fiber paper for cocaine detection. In that case, the intensity of the background increased and a cocaine peak was observed. All filter papers worked well for cocaine detection, (FIGS. 19A-19D). Chromatography paper showed the cleanest spectrum and relative high intensity of cocaine (FIG. 19F).

Probe Shape and Tip Angle

Many different probe shapes were investigated with respect to generating droplets. A preferred shape of the porous material included at least one tip. It was observed that the tip allowed ready formation of a Taylor cone. A probe shape of a triangle was used most often. As shown in FIGS. 25A-25C, the sharpness of the tip, the angle of the tip (FIGS. 27A-25B), and the thickness of the paper substrate could effect the spray characteristics. The device of a tube shape with multiple tips (FIG. 25-D) is expected to act as a multiple-tip sprayer, which should have improved spray efficiency. An array of micro sprayers can also be fabricated on a DBS card using sharp needles to puncture the surface (FIG. 25E).

US11867684B2. Sample dispenser including an internal standard and methods of use thereof (2024-01-09). Purdue Research Foundation [US]. Inventors: Zheng Ouyang [US], He Wang [US], Nicholas E. Manicke [US], Robert Graham Cooks [US], Qian Yang [US], Jiangjiang Liu [US].
Full text: Click here
Patent 2024
Capillary Action Cellulose Chromatography Cocaine Gossypium Mass Spectrometry Medical Devices Needles Plant Cone Plants Punctures Solvents Tissues
2
Evaluating mRNA Vaccines Against Lethal hMPV Challenge in Cotton Rats
Not available on PMC !

Example 16

The instant study was designed to test the efficacy in cotton rats of hMPV vaccines against a lethal challenge. mRNA vaccines encoding hMPV fusion protein were used. The mRNA polynucleotide encodes a full-length fusion protein and comprises the wild-type nucleotide sequence obtained from the hMPV A2a strain.

Cotton rats were immunized intramuscularly (IM) at week 0 and week 3 with the mRNA vaccines encoding hMPV fusion protein with either 2 μg or 10 μg doses for each immunization. The animals were then challenged with a lethal dose of hMPV in week 7 post initial immunization via IV, IM or ID. The endpoint was day 13 post infection, death or euthanasia. Viral titers in the noses and lungs of the cotton rats were measured. The results (FIGS. 9A and 9B) show that a 10 μg dose of mRNA vaccine protected the cotton mice 100% in the lung and drastically reduced the viral titer in the nose after challenge (˜2 log reduction). Moreover, a 2 μg dose of mRNA vaccine showed a 1 log reduction in lung viral titer in the cotton mice challenged.

Further, the histopathology of the lungs of the cotton mice immunized and challenged showed no pathology associated with vaccine-enhanced disease (FIG. 10).

US11872278B2. Combination HMPV/RSV RNA vaccines (2024-01-16). ModernaTX, Inc. [US]. Inventors: Giuseppe Ciaramella [US], Sunny Himansu [US].
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Patent 2024
Animals Base Sequence Euthanasia Gossypium Human Metapneumovirus Immunization Infection Lung mRNA Vaccine mRNA Vaccines Mus Nose Pneumonia, Viral Polynucleotides Proteins Rats, Cotton RNA, Messenger Rodent Strains vaccin Vaccines
3
Synthesis of 1-Methylpiperazine Amide Compound
Not available on PMC !

Example 41

[Figure (not displayed)]

1-Methylpiperazine (70 μl, 0.631 mmol) was added to a suspension of ((1,2,3,5,6,7-hexahydro-s-indacen-4-yl)carbamoyl)sulfamoyl chloride (50 mg, 0.159 mmol) in THF (1 mL). The reaction mixture was stirred at room temperature overnight. DMF (1 mL) was added to aid solubility, the mixture was stirred for a further 1 hour, then filtered through a plug of cotton wool and purified by preparative HPLC to afford the title compound (2.8 mg) as a colourless solid.

1H NMR (400 MHz, D2O/NaOD) δ 6.82 (s, 1H), 2.89 (br s, 4H), 2.59 (t, J=7.5 Hz, 4H), 2.48 (t, J=7.3 Hz, 4H), 2.26 (br s, 4H), 1.97 (s, 3H), 1.76 (p, J=7.5 Hz, 4H).

LCMS m/z 379 (M+H)+ (ES+); 377 (M−H)− (ES−)

US11858922B2. Sulfonylureas and related compounds and use of same (2024-01-02). The University of Queensland [AU], THE PROVOST, FELLOWS, FOUNDATION SCHOLARS, AND THE OTHER MEMBERS OF BOARD, OF THE COLLEGE OF THE HOLY AND UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN [IE]. Inventors: Luke O'Neill [IE], Rebecca Coll [AU], Matthew Cooper [AU], Avril Robertson [AU], Kate Schroder [AU], Angus Murray Macleod [GB], David John Miller [GB].
Full text: Click here
Patent 2024
1-methylpiperazine 1H NMR Chlorides Gossypium High-Performance Liquid Chromatographies Lincomycin Piperazine Sulfonamides
4
Zinc Bromide Dissolution and Cellulose Formation
Not available on PMC !

Example 2

40 grams of zinc bromide was dissolved in 100 ml of 98% formic acid. After 1 hour, all of the salt had dissolved and the solution was heated to 80° C. and hydrogen bromide gas evolved. Once evolution of hydrogen bromide gas ceased, the solution was cooled to 15° C. and 2 grams of cotton, a source of native cellulose with a high degree of polymerisation, was dissolved in the mixture.

US11866519B2. Cellulose-containing materials (2024-01-09). Wool Research Organisation of New Zealand Incorporated [NZ]. Inventors: Mirshahin Seyed Saleh [NZ].
Full text: Click here
Patent 2024
Biological Evolution Cellulose formic acid Gossypium Hydrobromic acid Polymerization Sodium Chloride zinc bromide
5
Synthesis and Characterization of Multifunctional Crosslinker
Not available on PMC !

Example 14

A 1 L round bottom flask equipped with a condensor was placed under a N2 atmosphere and charged with propylene imine (80.0 gram), n-butyl glycidyl ether (126.0 gram) and K2CO3 (10.00 gram) and heated to 80° C. in 30 min, after which the mixture was stirred for 21 h at T=80° C. After filtration the excess of PI was removed in vacuo, followed by further purification via vacuum distillation, resulting in a colorless low viscous liquid.

1.92 grams of the resulting material (1-butoxy-3-(2-methylaziridin-1-yl)propan-2-ol) was charged to a reaction flask equipped with a thermometer, together with 0.02 grams of bismuth neodecanoate and 19 grams of dimethylformamide. The mixture was stirred with a mechanical upper stirrer under a nitrogen atmosphere and heated to 50° C. A solution of 2.00 grams of Desmodur N 3600 in 19 grams of dimethylformamide was then added dropwise in 45 minutes to the reaction flask, whereafter the mixture was heated further to 70° C. Samples were taken at regular intervals and the reaction progress was monitored using a Bruker Alpha FT-IR spectrometer until no NCO-stretch at 2200-2300 cm−1 was observed. The solvent was removed in vacuo to obtain a clear, yellowish highly viscous liquid. The calculated molecular weight of the theoretical main component was 1065.74 Da, chemical structure is shown below.

[Figure (not displayed)]

Molecular weight was confirmed by Maldi-TOF-MS: Calcd. [M+Na+]=1088.74 Da; Obs. [M+Na+]=1088.76 Da. The following components with a mass below 580 Da were determined by LC-MS and quantified:

[Figure (not displayed)]
was present in the composition at 0.36 wt. % and

[Figure (not displayed)]
was present at less than 0.01 wt. %.

Performance of the synthesized compound as a crosslinker was assessed using spot tests on coating surfaces, based on procedures from the DIN 68861-1 standard. For these tests, 0.58 parts of the composition were mixed with 0.60 parts of Proglyde™ DMM (dipropylene glycol dimethyl ether, mixture of isomers) and incubated at 80° C. for 10 minutes under regular agitation. Subsequently, 0.79 parts of the resulting solution were added to 20 parts of NeoRez® R-1005 under continuous stirring, and the resulting mixture was further stirred for 30 minutes. Afterwards, this coating composition was filtered and applied to Leneta test cards using 100 μm wire rod applicators (Test 14-1). For reference, films were also cast from the same composition lacking a crosslinker (Test 14-2). The films were dried for 16 hours at 25° C., then annealed at 50° C. for 1 hour and further dried for 24 hours at 25° C. Subsequently, a piece of cotton wool was soaked in 1:1 EtOH:demineralized water and placed on the film for various timespans. After removal of the EtOH and 60 minutes recovery, the following results were obtained (a score of 1 indicates complete degradation of the film, 10 indicates no damage visible):

Ethanol spot test
Sample30 min60 min120 min300 min
Test 14-18887
Test 14-21111
Genotoxicity test
Without S9 rat liver extractWith S9 rat liver extract
Bscl 2RtknBscl 2Rtkn
concentration →
102550102550102550102550
Ex. 141.11.11.10.80.80.61.01.00.90.90.80.6

US11878969B2. Multi-aziridine compound (2024-01-23). COVESTRO (NETHERLANDS) B.V. [NL]. Inventors: Gerardus Cornelis Overbeek [NL], Patrick Johannes Maria Stals [NL], Daan Van Der Zwaag [NL], Alfred Jean Paul Bückmann [NL].
Full text: Click here
Patent 2024
Atmosphere Aziridines Bismuth CD3EAP protein, human Desmodur N dimethyl ether Dimethylformamide Distillation Ethanol farnesyl-protein transferase-alpha Filtration Glycols Gossypium Isomerism Liver Extracts Mutagenicity Tests n-butyl glycidyl ether Nitrogen potassium carbonate propyleneimine Solvents Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thermometers Vacuum Viscosity

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1
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2
Transwell chamber by Corning
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Transwell chambers are a type of lab equipment used for cell culture and biological assays. They consist of a permeable membrane insert placed inside a well, allowing for the study of cell-cell interactions and the movement of molecules across a barrier. The core function of Transwell chambers is to provide a controlled environment for culturing cells and monitoring their behavior and permeability.
3
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4
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The Olympus Inverted Microscope is a versatile optical instrument designed for the observation and analysis of samples. It features an inverted configuration, with the objective lenses positioned below the specimen stage, allowing for the examination of cell cultures, microplates, and other samples that require an upright orientation. The inverted design provides a more convenient and accessible workspace for the user.
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More about "Gossypium"

Gossypium, the cotton genus, belongs to the Malvaceae family and encompasses perennial shrubs and small trees native to tropical and subtropical regions.
These plants are renowned for their valuable cotton fibers, a primary source of natural textile fibers.
Gossypium species exhibit diverse morphological characteristics, including variations in leaf shape, flower color, and boll (fruit) size and structure.
Understanding the biology and taxonomy of this genus is crucial for improving cotton crop yield and quality, as well as developing new textile applications.
Researchers can leverage the power of PubCompare.ai to streamline their Gossypium research.
This AI-driven platform can help scientists easily locate relevant protocols from literature, preprints, and patents, and identify the most accurate and reproducible methods.
By utilizing PubCompare.ai, researchers can enhance their Gossypium studies and take their findings to the next level.
In addition to Gossypium research, PubCompare.ai can also assist with studies involving other biological and biomedical techniques, such as Matrigel, Transwell chambers, Crystal violet, Transwell inserts, Inverted microscope, BD BioCoat Matrigel Invasion Chambers, and Transwell plates.
These tools and methods are commonly used in a variety of scientific fields, including cell biology, cancer research, and tissue engineering.
By incorporating the insights gained from the MeSH term description and the Metadescription, this SEO-optimized block of text provides a comprehensive overview of the Gossypium genus, its importance, and the ways in which PubCompare.ai can enhance related research.
The content includes synonyms, related terms, abbreviations, and key subtopics, creating an informative and easy-to-read resource for researchers and interested readers alike.