Log In Sign up
The largest database of trusted experimental protocols
> Living Beings > Plant > Moringa

Moringa

Moringa, a versatile plant with a rich history of traditional use, has garnered increasing scientific interest due to its potential health benefits.
PubCompare.ai's innovative platform leverages AI to help users discover optimized research protocols, compare findings from literature, preprints, and patents, and unlock the full therapeutic potential of this remarkable botanical.
Our user-friendly tools enhance reproducibility and provide a concise, informative overveiw to guide your exploration of Moringa's diverse applications.

Most cited protocols related to «Moringa»

1
Optimized Moringa Seed Extract Preparation
Cited 5 times
Moringa seeds were obtained from the Jamaica Moringa Farmers’ Association (Kingston, Jamaica). A voucher specimen was prepared and submitted to the Rutgers University CHRB Chrysler Herbarium (Accession number: 146375). The preparation of MSE was optimized based on the degree of biotransformation of GLSs to ITCs in situ, in order to obtain the highest MIC-1 content. The optimization was performed prior to preparation of the extract for in vivo and in vitro studies. The development and optimization of the procedure for the preparation of MSE involved incubating ground seeds in water at a controlled temperature with constant agitation for a duration of time, after which ethanol was added at 4x the volume of water to arrest the myrosinase reaction (Table 1). Incubation temperatures were first evaluated at 25°C or 37°C using a solvent ratio of 1:4 (g seeds:mL H2O) and incubation time of 2 h. Then solvent ratio was evaluated at 1:2, 1:3, and 1:4 using an incubation temperature of 37°C and incubation time of 2 h. Incubation time was also evaluated at 0.5, 1 and 2 h using an incubation temperature of 37°C and a solvent ratio of 1:4. All experiments were performed in triplicates using 10 g of ground seeds. Seed/solvent slurries were then filtered through a Whatman filter paper, and filtrate was reduced in volume via rotary evaporation and dried via lyophilization. The freeze-dried extracts were weighed and analyzed for MIC-1 content by LC-MS (see below).
A large batch of MSE, obtained using optimized extraction conditions (1 g seed powder:3 mL water at 37°C for 2 h with constant agitation, followed by addition of 4x volume of ethanol, filtered and dried), was produced and stored at -20°C and used for all subsequent studies. The extract was analyzed and MIC-1 quantified by LC-MS. Nutritional analysis and fatty acid composition of MSE was performed by NJ Feed Labs (Trenton, NJ).
Jaja-Chimedza A., Graf B.L., Simmler C., Kim Y., Kuhn P., Pauli G.F, & Raskin I. (2017). Biochemical characterization and anti-inflammatory properties of an isothiocyanate-enriched moringa (Moringa oleifera) seed extract. PLoS ONE, 12(8), e0182658.
Full text: Click here
Publication 2017
Biotransformation Cardiac Arrest Ethanol Farmers Fatty Acids Freeze Drying Freezing Glucosinolates Moringa myrosinase Powder Solvents
2
Moringa Oleifera Leaf Extract Preparation
Cited 4 times
Leaves of Moringa Oleifera were received from Moringa Arava Ltd, Israel. Moringa Arava grows the Moringa Oleifera plant in the Dead Sea area, Israel, where it is grown in rich mineral soil. The plant derived aqueous extract tested in this study was prepared in our laboratory by mixing 1g dried and powdered leaves of Moringa Oleifera with 10 mL boiling water for 5 minutes. The mixture was then filtered twice through a 2 μm pore sterile filter paper into a sterile tube. The aqueous extract stock solution (100 mg/mL) was freshly prepared for each set of experiments and stored at 4°C for up to 5 days.
Berkovich L., Earon G., Ron I., Rimmon A., Vexler A, & Lev-Ari S. (2013). Moringa Oleifera aqueous leaf extract down-regulates nuclear factor-kappaB and increases cytotoxic effect of chemotherapy in pancreatic cancer cells. BMC Complementary and Alternative Medicine, 13, 212.
Full text: Click here
Publication 2013
Arava Minerals Moringa Moringa oleifera Plants Sterility, Reproductive
3
Dosage Optimization for Moringa Tea Diuretic Activity
Cited 3 times
Based on community use, the Moringa tea was prepared by soaking 2 teaspoons (Tsp) (about 2 g) of loose tea leaves in 100 mL of water (0.68 g dried tea extract) which is to be consumed by an adult with the average weight considered to be 60 kg. Based on this previous information, the effective human dose is calculated to be 11.33 mg/kg, from which the animal dose was derived. This dose was then halved (1 Tsp), doubled (4 Tsp), and tripled (6 Tsp) to explore the diuretic activity of the leaves at different dose levels in rats. The dose calculation was based on slight modifications of the US FDA recommendations for dose extrapolation between species.15
Animal dose=Human dose×Conversion factor
Animal dose=11.33mg/kg×6.17
Dose for Group I=34.95mg/kg(M.stenopetalaleaf infusion[MLI]1based on1Tsp/100mL)
Dose for Group II=69.91mg/kg(MLI2based on2Tsp/100mL)
Dose for Group III=139.81mg/kg(MLI3based on4Tsp/100mL)
Dose for Group IV=279.62mg/kg(MLI4based on6Tsp/100mL)
The weight of tea leaves to be measured was determined based on the total weight of the respective group of animals and the final administration volume of 5 mL/kg. A total volume of 100 mL was prepared as follows: the loose tea leaves of M. stenopetala were macerated in hot distilled water (94.5°C) for 15 minutes which was filtered through cotton gauze to give clear straw-colored infusions with different depths of colors. The dose for the aqueous extract was determined based on a previous animal diuretic study that was conducted with the hydroalcoholic leaf extract of M. stenopetala.13 Accordingly, 500 mg/kg which was observed to be an optimal dose served as the maximum dose from which the other dose levels were proportionally derived (250, 125, and 62.5 mg/kg).
Fekadu N., Basha H., Meresa A., Degu S., Girma B, & Geleta B. (2017). Diuretic activity of the aqueous crude extract and hot tea infusion of Moringa stenopetala (Baker f.) Cufod. leaves in rats. Journal of Experimental Pharmacology, 9, 73-80.
Publication 2017
Adult Animals Diuretics Gossypium Homo sapiens Moringa Plant Leaves Rattus
4
Moringa Stenopetala Leaf Extract Evaluation
Cited 3 times
The normal mice were divided into five groups and each group contained six animals. The control group was fed with distilled water. Other groups were fed orally with three different doses of aqueous ethanol extract of Moringa stenopetala leaves (250, 500 and 750 mg/kg). For the last group, acarbose (3 mg/kg) was used as a positive control. All treatments were administered to the mice 5 min before loading sucrose (3 g/kg). Blood samples were collected from a tail vein at 0, 30, 60, 90 and 180 min. The blood glucose levels were determined by glucose oxidase method.
Toma A., Makonnen E., Mekonnen Y., Debella A, & Adisakwattana S. (2015). Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats. BMC Complementary and Alternative Medicine, 15, 242.
Full text: Click here
Publication 2015
Acarbose Animals BLOOD Blood Glucose Ethanol Mice, House Moringa Oxidase, Glucose Sucrose Tail Veins
5
Antifungal Activity of Moringa Extracts
Cited 2 times
The impact of Moringa extracts to represent zone inhibition against the fungi growth on solid media was determined regarding six toxigenic strains of fungi, four out of them were belonged to the Aspergillus genus. This experiment was done using the same methodology described by Shehata et al. [30 (link)]. Briefly, Autoclaved Potato dextrose agar was poured into sterile Petri dishes (15 cm), five-millimeter diameter wells were made in the four principal directions of the plate using cork-porer tools. Each well was filled with 100 μL extract and a paper disk of fungi spores was inoculated to the plate center. The plates were incubated (28 °C/96 h), where the zone diameter of inhibition was measured in millimeters.
Abu-Sree Y.H., Abdel-Fattah S.M., Abdel-Razek A.G, & Badr A.N. (2021). Neoteric approach for peanuts biofilm using the merits of Moringa extracts to control aflatoxin contamination. Toxicology Reports, 8, 1685-1692.
Full text: Click here
Publication 2021
Agar Aspergillus Fungi Glucose Hyperostosis, Diffuse Idiopathic Skeletal Moringa Psychological Inhibition Solanum tuberosum Spores, Fungal Sterility, Reproductive Strains

Most recents protocols related to «Moringa»

1
FTIR Spectroscopy of Moringa and Ag-NPs
The freeze-dried crude aqueous extracts of Moringa and also the Ag-NPs powders were prepared in the central lab of the Nawah foundation. The samples were prepared by milling with anhydrous potassium bromide (KBr) to form very fine powders. Then, the powders were compressed into two thin pellets for analysis. The infrared spectra were recorded with a Fourier transform infrared (FTIR) spectroscopy analyzer (Model JASCO FTIR-6100) within the scanning range 4000–400 cm−1. The spectra were smoothed using 3 or 5 points and the baseline of the spectra was corrected using the previously recorded spectra of the sample [60 (link)] (Figure 3 and Figure 4).
Ahmed M., Marrez D.A., Abdelmoeen N.M., Mahmoud E.A., Abdel-Shakur Ali M., Decsi K, & Tóth Z. (2023). Proximate Analysis of Moringa oleifera Leaves and the Antimicrobial Activities of Successive Leaf Ethanolic and Aqueous Extracts Compared with Green Chemically Synthesized Ag-NPs and Crude Aqueous Extract against Some Pathogens. International Journal of Molecular Sciences, 24(4), 3529.
Full text: Click here
Publication 2023
Complex Extracts Freezing Moringa Pellets, Drug Powder Spectroscopy, Fourier Transform Infrared
2
Optical Properties of Biosynthesized Silver Nanoparticles
The optical properties of Ag-NPs were monitored by UV–VIS absorption spectroscopy (PG Instruments Ltd. T80 UV–VIS spectrophotometer, Loughborough, UK) in the central lab of the Biochemistry Department, Faculty of Agriculture, Cairo University. UV–VIS spectra were recorded in the wavelength range of 300–700 nm.
The UV–VIS spectrum of Ag-NPs in Figure 1 was adjusted from the reaction medium as an incubation time function. Ag-NPs from Moringa gave absorption peaks between 300 and 700 nm. With time, the absorption intensity increased, indicating an increase in the quantity of formed Ag-NPs. The characteristic feature of the peak at 400–500 nm, which was checked in the UV–VIS spectrum, validated the synthesis of Ag-NPs. This single peak of the plasmon surface resonance indicated that the Ag-NPs were spheres with a wide distribution of size. The UV–VIS spectrum of synthesized Ag-NPs gave absorbance at 472 nm after 24 h of incubation in the dark.
Ahmed M., Marrez D.A., Abdelmoeen N.M., Mahmoud E.A., Abdel-Shakur Ali M., Decsi K, & Tóth Z. (2023). Proximate Analysis of Moringa oleifera Leaves and the Antimicrobial Activities of Successive Leaf Ethanolic and Aqueous Extracts Compared with Green Chemically Synthesized Ag-NPs and Crude Aqueous Extract against Some Pathogens. International Journal of Molecular Sciences, 24(4), 3529.
Full text: Click here
Publication 2023
Anabolism Faculty Moringa Spectrum Analysis Surface Plasmon Resonance Vision
3
Antimicrobial Evaluation of Moringa Extracts
MIC was calculated using the tube dilution technique [63 (link),64 (link)]. To achieve 108 CFU mL−1 inocula, a 24 h culture of the tested bacterial species was diluted in 10 mL of tryptic soy broth (TSB) according to the 0.5 McFarland standard. Ten different Moringa extract concentrations, ranging from 5000 µg/mL to 10.0 µg/mL, were made in culture tubes with DMSO. Each tube received 0.1 mL of bacterial cell suspension as an inoculant, and it was then incubated for 24 h at 37 °C. Turbidity in the broth served as a sign of inoculum growth, and the extract concentration at which the test organism’s bacterial growth was inhibited at the lowest level was deemed to be the minimal inhibitory concentration (MIC). MIC was carried out against the fungus by the protocol that was designed [65 (link),66 (link)]. Different quantities of Moringa extracts were separately diluted in 0.5 mL of 0.1% Tween 80 (Merck, Darmstadt, Germany), combined with 9.5 mL of melting PDA, and then placed into a Petri dish (6 cm). A 3-microliter fungal suspension was used to centrally inoculate the prepared plates (108 CFU mL−1, 0.5 McFarland standard). The plates were incubated at 25 °C for 24–48 h.
Ahmed M., Marrez D.A., Abdelmoeen N.M., Mahmoud E.A., Abdel-Shakur Ali M., Decsi K, & Tóth Z. (2023). Proximate Analysis of Moringa oleifera Leaves and the Antimicrobial Activities of Successive Leaf Ethanolic and Aqueous Extracts Compared with Green Chemically Synthesized Ag-NPs and Crude Aqueous Extract against Some Pathogens. International Journal of Molecular Sciences, 24(4), 3529.
Full text: Click here
Publication 2023
Bacteria Cardiac Arrest Cells Fungi Hyperostosis, Diffuse Idiopathic Skeletal Minimum Inhibitory Concentration Moringa Sulfoxide, Dimethyl tryptic soy broth Tween 80
4
Morphological Screening of Silver Nanoparticles
A transmission electron microscope (JEM-1400, JEOL model) was used to determine the morphological screening of Ag-NPs. Imaging was recorded by the electronic microscope lab of Cairo University Research Park (CURP). A glow-discharged grid of carbon was used to place freshly prepared Ag-NPs on and left to be air-dried for a few minutes. Then, the shape and surface harshness of the nanocomposite specimens were checked by a TEM-operated system. TEM of the synthesized Ag-NPs by the crude aqueous extract from Moringa and NaBH4 are shown in Figure 2. They ranged from 4.40 to 12.2 nm in size and they were spherical.
Ahmed M., Marrez D.A., Abdelmoeen N.M., Mahmoud E.A., Abdel-Shakur Ali M., Decsi K, & Tóth Z. (2023). Proximate Analysis of Moringa oleifera Leaves and the Antimicrobial Activities of Successive Leaf Ethanolic and Aqueous Extracts Compared with Green Chemically Synthesized Ag-NPs and Crude Aqueous Extract against Some Pathogens. International Journal of Molecular Sciences, 24(4), 3529.
Full text: Click here
Publication 2023
Carbon Complex Extracts Electron Microscopy Moringa Transmission Electron Microscopy
5
Moisture Content in Moringa Leaves
The moisture content in Moringa leaves was determined at 105 °C in an oven [57 ].
Ahmed M., Marrez D.A., Abdelmoeen N.M., Mahmoud E.A., Abdel-Shakur Ali M., Decsi K, & Tóth Z. (2023). Proximate Analysis of Moringa oleifera Leaves and the Antimicrobial Activities of Successive Leaf Ethanolic and Aqueous Extracts Compared with Green Chemically Synthesized Ag-NPs and Crude Aqueous Extract against Some Pathogens. International Journal of Molecular Sciences, 24(4), 3529.
Full text: Click here
Publication 2023
Moringa

Top products related to «Moringa»

1
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
2
Gallic acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
3
Folin ciocalteu reagent by Merck Group
Sourced in United States, Germany, Italy, India, France, Poland, Spain, China, Chile, Sao Tome and Principe, United Kingdom, Switzerland, Australia, Brazil, Canada, Singapore, Portugal, Mexico, Malaysia, New Zealand, Macao, Croatia, Belgium, Lithuania, Romania, Argentina, Finland
The Folin-Ciocalteu reagent is a colorimetric reagent used for the quantitative determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes that undergo a color change when reduced by phenolic compounds.
4
Tween 80 by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, France, Sao Tome and Principe, Spain, Poland, China, Belgium, Brazil, Switzerland, Canada, Australia, Macao, Ireland, Chile, Pakistan, Japan, Denmark, Malaysia, Indonesia, Israel, Saudi Arabia, Thailand, Bangladesh, Croatia, Mexico, Portugal, Austria, Puerto Rico, Czechia
Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
5
Whatman no 1 filter paper by Cytiva
Sourced in United Kingdom, Germany, United States, Switzerland, India, Japan, China, Australia, France, Italy, Brazil
Whatman No. 1 filter paper is a general-purpose cellulose-based filter paper used for a variety of laboratory filtration applications. It is designed to provide reliable and consistent filtration performance.
6
Whatman filter paper by Cytiva
Sourced in United Kingdom, United States, Japan, Germany, Switzerland, China, India
Whatman filter paper is a laboratory filtration product designed for various filtering applications. It is manufactured to provide consistent quality and performance. The core function of Whatman filter paper is to separate solid particles from liquids or gases through the process of filtration.
7
Trolox by Merck Group
Sourced in United States, Germany, Italy, France, Spain, Brazil, India, Poland, Switzerland, Canada, China, New Zealand, United Kingdom, Chile, Macao, Mexico, Australia, Portugal, Czechia, Hungary, Japan, Belgium, Singapore, Argentina, Ireland, Sao Tome and Principe, Denmark, Thailand, Israel, Morocco, Cameroon
Trolox is a water-soluble vitamin E analog that functions as an antioxidant. It is commonly used in research applications as a reference standard for measuring antioxidant capacity.
8
Allyl isothiocyanate by Merck Group
Sourced in United States, Germany, United Kingdom, Australia
Allyl isothiocyanate is a colorless, pungent-smelling liquid chemical compound. It is an organic compound with the molecular formula C4H5NS. Allyl isothiocyanate is commonly used as a laboratory reagent.
9
Chloroform by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, Spain, France, Canada, Switzerland, China, Australia, Brazil, Poland, Ireland, Sao Tome and Principe, Chile, Japan, Belgium, Portugal, Netherlands, Macao, Singapore, Sweden, Czechia, Cameroon, Austria, Pakistan, Indonesia, Israel, Malaysia, Norway, Mexico, Hungary, New Zealand, Argentina
Chloroform is a colorless, volatile liquid with a characteristic sweet odor. It is a commonly used solvent in a variety of laboratory applications, including extraction, purification, and sample preparation processes. Chloroform has a high density and is immiscible with water, making it a useful solvent for a range of organic compounds.
10
Phosphate buffer by Merck Group
Sourced in United States, Germany, India, United Kingdom, Spain
Phosphate buffer is a commonly used buffer solution that maintains a stable pH. It is a mixture of sodium phosphate and potassium phosphate salts that helps maintain a consistent pH in aqueous solutions, typically in the range of 6.0 to 8.0. The buffer system is widely used in various laboratory applications that require a controlled pH environment.

More about "Moringa"

Moringa, also known as the 'miracle tree', is a versatile and highly nutritious plant that has a rich history of traditional use.
This remarkable botanical has garnered increasing scientific interest due to its potential health benefits, which include antioxidant, anti-inflammatory, and antimicrobial properties.
PubCompare.ai's innovative platform leverages advanced AI technology to help users discover optimized research protocols, compare findings from literature, preprints, and patents, and unlock the full therapeutic potential of Moringa.
The user-friendly tools provided by PubCompare.ai enhance reproducibility and provide a concise, informative overview to guide your exploration of Moringa's diverse applications.
Some of the key compounds and techniques associated with Moringa research include Methanol, a common solvent used for extract preparation; Gallic acid, a phenolic compound with antioxidant activity; Folin-Ciocalteu reagent, a colorimetric assay used to measure total phenolic content; Tween 80, a surfactant used in formulations; Whatman No. 1 filter paper, a commonly used filtration medium; Trolox, a water-soluble vitamin E analog used as a standard in antioxidant assays; Allyl isothiocyanate, a volatile compound with antimicrobial properties; Chloroform, a solvent used for extraction; and Phosphate buffer, a common buffer system used in various assays.
By leveraging the insights and tools provided by PubCompare.ai, researchers and healthcare professionals can unlock the full potential of Moringa and explore its diverse applications in areas such as nutrition, medicine, and personal care.
Whether you're interested in Moringa's antioxidant properties, its anti-inflammatory effects, or its potential as a natural antimicrobial, PubCompare.ai's platform can help you navigate the wealth of information and find the most relevant and optimized research protocols to advance your Moringa-related studies.