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Psidium guajava

Psidium guajava, commonly known as the guava, is a small tree or shrub native to tropical America.
It is a widely cultivated fruit crop valued for its edible fruit and various medicinal properties.
Psidium guajava has been the subject of extensive research, with studies exploring its phytochemistry, pharmacological activities, and potential therapeutic applications.
PubCompare.ai's AI-powered platform can enhance Psidium guajava research by helping researchers locate relevant protocols from literature, preprints, and patents, while performing intelligent comparisons to identify the best protocols and products.
This can improve reproducibility and accuracy in Psidium guajava studies, advancing our understanding of this important plant species.

Most cited protocols related to «Psidium guajava»

1
Antibody-Dependent Cell-Mediated Cytotoxicity Assay
Cited 46 times
Antibody Dependent Cellular Cytotoxic activity mediated by the patient plasma/sera and the HIVIG polyclonal IgG preparation was detected according to our modification of the previously described GranToxiLux (GTL) cell-mediated cytotoxicity procedure (23 (link),24 (link)). The assay was performed in 96-well plates as follows and outlined in Figure 1A. Infected and uninfected target cells were counted, washed, resuspended in R10 at 1×106 cell/ml, and labeled with a fluorescent target-cell marker (TFL4; OncoImmunin, Inc., Gaithersburg, MD) and a viability marker (NFL1; OncoImmunin, Inc.) for 15 minutes in a 37°C water bath as specified by manufacturer. After two washes using 10 ml of R10, viable cells were counted using a Guava PCA (Millipore, Billerica, MA) and adjusted to reach a final viable effector to viable target ratio of 30:1 or 10:1 when PBMC and NK cells were used as effector cells, respectively. Twenty-five μl of each effector and target cell suspension and 75 μl of GzB substrate (OncoImmunin, Inc.) were dispensed into each well of a 96-well V-bottom plate. After incubation for 5 minutes at room temperature (RT), 25μl of the appropriate antibody or IgG dilutions were added to the target/effector cell suspension and incubated for 15 minutes at RT. The plates were subsequently centrifuged for 1 minute at 300 × g, and incubated for 1 hour at 37°C and 5% CO2. After two washes with WB, cells were resuspended in 225μl of WB, placed at 4°C, and acquired directly from the assay plate with the LSRII (BD Bioscience, San Jose, California) within 6 hours using the High-Throughput-System (HTS, BD Bioscience, San Jose, California). A minimum of 2.5×103 and 5×103 events representing viable gp120-coated and infected target cells, respectively, was acquired for each condition. The signal for each fluorophore was detected using: 1) 640nm/40mW laser and 660/20 filter for TFL4; 405nm/50mW laser and 450/50 filter for NFL1; 3) 488nm/20mW laser and the combination of 505LP with 525/50 filters for the GzB substrate. Because of the spectral properties of the fluorescent molecules utilized in this panel, manual compensation of the signals was not required to analyze the data, as previously reported (24 (link)). Data analysis was performed using FlowJo 8.8.4 software. The investigators developed a dedicated analysis template that reflects the gating strategy illustrated in Figure 1B.
Pollara J., Hart L., Brewer F., Pickeral J., Packard B., Hoxie J.A., Komoriya A., Ochsenbauer C., Kappes J.C., Roederer M., Huang Y., Weinhold K.J., Tomaras G.D., Haynes B.F., Montefiori D.C, & Ferrari G. (2011). High throughput quantitative analysis of HIV-1 and SIV-specific ADCC-mediating antibody responses. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 79(8), 603-612.
Publication 2011
A 103 Bath Biological Assay Cells Cytotoxin HIV Envelope Protein gp120 HIV hyperimmune globulin Immunoglobulins Natural Killer Cells Patients Plasma Psidium guajava Serum Technique, Dilution
2
Cell Permeabilization and BRET Assay
Cited 31 times
Cells were washed with DPBS, harvested by trituration, and transferred to opaque black or white 96-well plates containing diluted drug solutions. For assays with nucleotide-free heterotrimers (Fig. 4), cells were washed with permeabilization buffer containing 140 mm KCl, 10 mm NaCl, 1 mm MgCl2, 0.1 mm K-EGTA, 20 mm NaHEPES (pH 7.2), harvested by trituration, and permeabilized in the same buffer containing 10 μg ml−1 high purity digitonin (EMD Millipore, Burlington, MA). Measurements were made from permeabilized cells supplemented either with GDP (0.5 mm) or apyrase (2 units ml−1; Sigma) and agonist. BRET and luminescence measurements were made using a Mithras LB940 photon-counting plate reader (Berthold Technologies GmbH, Bad Wildbad, Germany). Coelenterazine h (5 μm; Nanolight, Pinetop, AZ) or furimazine (Nano-Glo; 1:1000, Promega Corp.) were added to all wells immediately prior to making measurements with Rluc8 and Nluc (or Nluc fragments), respectively. Raw BRET signals were calculated as the emission intensity at 520–545 nm divided by the emission intensity at 475–495 nm. Net BRET was this ratio minus the same ratio measured from cells expressing only the BRET donor. NES–venus–mG fluorescence in Fig. 2 was measured using a Guava 6HT/2L flow cytometer (excitation 488 nm, detection 525/30 nm) and reported as average fluorescence from all positive cells.
Wan Q., Okashah N., Inoue A., Nehmé R., Carpenter B., Tate C.G, & Lambert N.A. (2018). Mini G protein probes for active G protein–coupled receptors (GPCRs) in live cells. The Journal of Biological Chemistry, 293(19), 7466-7473.
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Publication 2018
Apyrase Biological Assay Buffers Cells coelenterazine Digitonin Egtazic Acid Fluorescence furimazine Luminescent Measurements Magnesium Chloride Nucleotides Pharmaceutical Solutions Promega Psidium guajava Sodium Chloride Tissue Donors
3
Murine Leukemia Cell Line Engineering
Cited 21 times
All of the cell lines used in this study were tested for mycoplasma and were negative. RN2c cells were derived by retroviral transduction of a murine MLL-AF9/NrasG12D acute myeloid leukemia cell line (RN2)11 with MSCV-hCas9-PGK-Puro, followed by puromycin selection and serial dilution to derive single cell-derived clones. Clones were screened by anti-flag Western blotting for high levels of stable Cas9 expression. Multiple independent clones displayed a similar CRISPR editing efficiency as RN2c. RN2c were cultured in RPMI1640 supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin. 38B9 cells were cultured in RPMI1640 supplemented with 10% FBS and 0.055 mM 2-mercaptoethanol. NIH3T3 cells were cultured in DMEM supplemented with 10% fetal calf serum and penicillin/streptomycin. Ecotropic Plat-E cells and HEK293T cells were cultured in DMEM supplemented with 10% FBS and penicillin/streptomycin. Plat-E cells were used for retroviral delivery of hCas9 or RPA3 cDNA expression vectors, following standard procedures27 (link).
sgRNA competition assays were performed using the U6-sgRNA-EFS-GFP or the U6-sgRNA-EFS-mCherry plasmids, where indicated. These plasmids were used to generate lentivirus by transfecting HEK293T cells with sgRNA:pVSVg:psPAX2 plasmids in a 4:2:3 ratio using PEI reagent (Polysciences : #23966). Viral supernatants were collected between the 36 and 72 hour timepoints following transfection. All transfections and viral collections were performed in 96 well plates to allow the evaluation of large numbers of sgRNAs systematically in a one-by-one manner. For sgRNA/GFP competition assays, flow cytometry analysis was performed on 96 well plates of cells using a Guava Easycyte HT instrument (Millipore). Gating was performed on live cells using forward and side scatter, prior to measuring of GFP positivity.
For the Rpa3 sgRNA/cDNA rescue experiment, RN2c were first transduced with empty or RPA3 MigR1, followed by transduction with Rpa3 sgRNAs expressed using the U6-sgRNA-EFS-mCherry vector. Gating was performed on GFP+/mCherry+ cells to evaluate rescue.
Shi J., Wang E., Milazzo J.P., Wang Z., Kinney J.B, & Vakoc C.R. (2015). Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Nature biotechnology, 33(6), 661-667.
Publication 2015
2-Mercaptoethanol Biological Assay Cell Lines Cells Clone Cells Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats DNA, Complementary Fetal Bovine Serum Flow Cytometry Lentivirus Leukemia, Myelocytic, Acute Mus Mycoplasma NIH 3T3 Cells Obstetric Delivery Penicillins Plasmids PLAT protein, human Psidium guajava Puromycin Retroviridae RPA3 protein, human Streptomycin Technique, Dilution Transfection
4
Murine Leukemia Cell Line Engineering
Cited 21 times
All of the cell lines used in this study were tested for mycoplasma and were negative. RN2c cells were derived by retroviral transduction of a murine MLL-AF9/NrasG12D acute myeloid leukemia cell line (RN2)11 with MSCV-hCas9-PGK-Puro, followed by puromycin selection and serial dilution to derive single cell-derived clones. Clones were screened by anti-flag Western blotting for high levels of stable Cas9 expression. Multiple independent clones displayed a similar CRISPR editing efficiency as RN2c. RN2c were cultured in RPMI1640 supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin. 38B9 cells were cultured in RPMI1640 supplemented with 10% FBS and 0.055 mM 2-mercaptoethanol. NIH3T3 cells were cultured in DMEM supplemented with 10% fetal calf serum and penicillin/streptomycin. Ecotropic Plat-E cells and HEK293T cells were cultured in DMEM supplemented with 10% FBS and penicillin/streptomycin. Plat-E cells were used for retroviral delivery of hCas9 or RPA3 cDNA expression vectors, following standard procedures27 (link).
sgRNA competition assays were performed using the U6-sgRNA-EFS-GFP or the U6-sgRNA-EFS-mCherry plasmids, where indicated. These plasmids were used to generate lentivirus by transfecting HEK293T cells with sgRNA:pVSVg:psPAX2 plasmids in a 4:2:3 ratio using PEI reagent (Polysciences : #23966). Viral supernatants were collected between the 36 and 72 hour timepoints following transfection. All transfections and viral collections were performed in 96 well plates to allow the evaluation of large numbers of sgRNAs systematically in a one-by-one manner. For sgRNA/GFP competition assays, flow cytometry analysis was performed on 96 well plates of cells using a Guava Easycyte HT instrument (Millipore). Gating was performed on live cells using forward and side scatter, prior to measuring of GFP positivity.
For the Rpa3 sgRNA/cDNA rescue experiment, RN2c were first transduced with empty or RPA3 MigR1, followed by transduction with Rpa3 sgRNAs expressed using the U6-sgRNA-EFS-mCherry vector. Gating was performed on GFP+/mCherry+ cells to evaluate rescue.
Shi J., Wang E., Milazzo J.P., Wang Z., Kinney J.B, & Vakoc C.R. (2015). Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Nature biotechnology, 33(6), 661-667.
Publication 2015
2-Mercaptoethanol Biological Assay Cell Lines Cells Clone Cells Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats DNA, Complementary Fetal Bovine Serum Flow Cytometry Lentivirus Leukemia, Myelocytic, Acute Mus Mycoplasma NIH 3T3 Cells Obstetric Delivery Penicillins Plasmids PLAT protein, human Psidium guajava Puromycin Retroviridae RPA3 protein, human Streptomycin Technique, Dilution Transfection
5
Transient Gene Delivery via Electroporation
Cited 11 times

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Publication 2011
Clone Cells Electroporation Therapy Flow Cytometry Genes hygromycin A N-fluoresceinylphosphatidylethanolamine Psidium guajava Pulses Short Hairpin RNA

Most recents protocols related to «Psidium guajava»

1
Cell Viability Assay with PI/HFS
HCT116 cells (40 000 cells per well) were treated with the test compounds (6c, 6m) for 72 h in the concentration range of 5, 10 and 20 μM. After treatment cells were washed with DPBS (Sigma; modified, without calcium chloride and magnesium chloride) and trypsinized using a trypsin–EDTA solution (Gibco™, 0.05%). After additional washing with DPBS, cells were stained at +4 °C overnight using PI/HFS solution (50 μg mL−1).67 (link) Samples were then analysed using Guava® easyCyte™ 8HT (Merck Millipore) flow cytometer with Guava Clean 3.1 software. The amount of debris was in the range of 9.2 ± 2.0%.
Dzedulionytė K., Fuxreiter N., Schreiber-Brynzak E., Žukauskaitė A., Šačkus A., Pichler V, & Arbačiauskienė E. (2023). Pyrazole-based lamellarin O analogues: synthesis, biological evaluation and structure–activity relationships. RSC Advances, 13(12), 7897-7912.
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Publication 2023
Calcium chloride Cells Edetic Acid HCT116 Cells Magnesium Chloride Psidium guajava Trypsin
2
Flow Cytometry Analysis of BALF Cells
BALF cell composition was determined by flow cytometric analysis of recovered lavage cells in suspension and stained with surface markers. In brief, BALF was centrifuged for 2 min at 2000 rpm, the supernatant removed, and the cell pellet resuspended and washed in 1 ml of FACS buffer (Phosphate Buffered Saline, 5% fetal bovine serum, 0.05% sodium azide). The washed pellet was resuspended and stained in a solution containing FACS buffer with 10% rat serum, Fc-receptor blocking antibody (clone 24G2) and the following antibodies [BD BioScience]: rat-anti-mouse Ly-6G-PECy7, rat-anti-mouse Siglec-F-PE, pan-leukocyte rat-anti mouse CD45-PerCP, and rat-anti-mouse CD11c-APC. Neutrophils were defined as CD45hi Ly6G+, eosinophils were defined as CD45hiLy6G− CD11c− SiglecF+, and alveolar macrophages were defined as CD45hi Ly6G− CD11c+SiglecF+. Flow cytometry was performed on a Guava EasyCyte 8HT (EMD Millipore).
Bhattacharya S., Maupin A.J., Schlosser A.G., Füchtbauer E.M., Gloria Y.C., Weber A.N., Holmskov U., Moeller J.B, & Templeton S.P. (2023). The role of FIBCD1 in response to Aspergillus fumigatus in lung epithelial cells. PLOS ONE, 18(3), e0282347.
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Publication 2023
Antibodies Antibodies, Blocking Buffers Cells Clone Cells Eosinophil Fc Receptor Fetal Bovine Serum Flow Cytometry Leukocytes Macrophages, Alveolar Mus Neutrophil Phosphates Psidium guajava Saline Solution Serum Sialic Acid Binding Immunoglobulin-like Lectins Sodium Azide
3
Apoptosis Assay of Breast Cancer Cells
The MDA-MB-231, SUM149 and SUM159 cell lines (5x104 cells/well) were seeded in 6-well plates and treated with GO (IC50: 3.78, 1.85 and 1.60 mmol/l, respectively) for 24 h. The cells were then collected and stained using the Annexin V/PI kit (Shanghai Yeasen Biotechnology, Co., Ltd.) for 15 min in the dark at 4˚C. The samples were tested using a Guava EasyCyte Plus flow cytometer (Merck KGaA) and FlowJo VX (Becton, Dickinson and Company) was used to analyze the results. The test was repeated three times.
Rong P., Yanchu L., Nianchun G., Qi L, & Xianyong L. (2023). Glyoxal-induced disruption of tumor cell progression in breast cancer. Molecular and Clinical Oncology, 18(4), 26.
Publication 2023
Annexin A5 Cell Lines Cells Psidium guajava
4
Cell Cycle Analysis of Cancer Cell Lines
The MDA-MB-231, SUM149 and SUM159 cell lines (5x104 cells/well) were seeded in 6-well plates and treated with GO (IC50: 3.78, 1.85 and 1.60 mmol/l, respectively) for 24 h. Next, the cells were collected and stored in pre-cooled alcohol overnight at 4˚C, then stained with PI (Shanghai Yeasen Biotechnology, Co., Ltd.) for 15 min in the dark at 4˚C. The samples were tested using a Guava EasyCyte Plus flow cytometer (Merck KGaA) and FlowJo VX (Becton-Dickinson and Company) was used to analyze the results. The test was repeated three times.
Rong P., Yanchu L., Nianchun G., Qi L, & Xianyong L. (2023). Glyoxal-induced disruption of tumor cell progression in breast cancer. Molecular and Clinical Oncology, 18(4), 26.
Publication 2023
Cell Lines Cells Ethanol Psidium guajava
5
Cell Cycle Analysis by Flow Cytometry
Cells were fixed with 70% ethanol, resuspended in the Guava® Cell Cycle Reagent Kit (#4500-0220, Luminex Corporation, Hayward, CA, USA) and analyzed with the Guava easyCyte flow cytometer (Luminex Corporation) and the InCyte software module (Luminex Corporation).
Ortiz-Rivera J., Nuñez R., Kucheryavykh Y, & Kucheryavykh L. (2023). The PYK2 inhibitor PF-562271 enhances the effect of temozolomide on tumor growth in a C57Bl/6-Gl261 mouse glioma model. Journal of Neuro-Oncology, 161(3), 593-604.
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Publication 2023
Cell Cycle Cells Ethanol Psidium guajava

Top products related to «Psidium guajava»

1
Guava easycyte flow cytometer by Merck Group
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The Guava EasyCyte flow cytometer is a compact and user-friendly instrument designed for automated cell analysis. It utilizes flow cytometry technology to provide rapid and accurate measurements of various cellular parameters, such as size, granularity, and fluorescence intensity. The Guava EasyCyte is capable of analyzing a wide range of cell types, including bacteria, yeast, and mammalian cells, making it a versatile tool for diverse applications in the life sciences and biomedical research.
2
Guava easycyte by Merck Group
Sourced in United States, Germany, United Kingdom, Italy
The Guava easyCyte is a compact, automated flow cytometry system designed for analyzing and quantifying cells and particles. It provides reliable and reproducible data for a variety of applications, including cell counting, phenotyping, and apoptosis analysis.
3
Guava easycyte ht by Merck Group
Sourced in United States, Germany, United Kingdom
The Guava easyCyte HT is a compact benchtop flow cytometer designed for rapid, high-throughput sample analysis. It features automated sample handling and data acquisition capabilities, allowing for efficient processing of multiple samples. The instrument measures various cellular parameters such as size, granularity, and fluorescence intensity, providing data for cell analysis and sorting applications.
4
Guava flow cytometer by Merck Group
Sourced in United States, Germany
The Guava flow cytometer is a compact and efficient instrument designed for cell analysis and counting. It utilizes the principles of flow cytometry to rapidly measure and analyze the physical and fluorescent characteristics of cells or other particles suspended in a fluid. The Guava flow cytometer provides accurate and reliable data for a variety of applications, including cell cycle analysis, immunophenotyping, and apoptosis detection.
5
Guava nexin reagent by Merck Group
Sourced in United States, Germany, United Kingdom
Guava Nexin Reagent is a laboratory product manufactured by Merck Group. It is a reagent used for flow cytometry analysis to detect and quantify apoptosis in cell samples.
6
Guava cell cycle reagent by Merck Group
Sourced in United States, Germany, Japan
The Guava Cell Cycle reagent is a laboratory product designed for use in cell cycle analysis. It provides a standardized method for measuring the DNA content of cells, which is a key indicator of the stage of the cell cycle. The reagent contains a fluorescent dye that binds to DNA, allowing for the quantification of cellular DNA content using flow cytometry or other compatible analytical instruments.
7
Guava easycyte 8ht by Merck Group
Sourced in United States, Germany, United Kingdom
The Guava easyCyte 8HT is a benchtop flow cytometer that can analyze up to 8 fluorescent parameters simultaneously. It is designed for quantitative analysis of cells and particles in a variety of biological samples.
8
Incyte software by Merck Group
Sourced in United States, Germany, United Kingdom
InCyte is a software product developed by Merck Group. It is designed to assist in the analysis and management of data acquired from laboratory equipment and experiments.
9
Guava easycyte 8ht flow cytometer by Merck Group
Sourced in United States, Germany
The Guava easyCyte 8HT flow cytometer is a laboratory instrument that performs cell analysis. It is capable of measuring various parameters of individual cells, such as size, granularity, and fluorescence intensity. The device can process up to 96 samples simultaneously, providing high-throughput analysis capabilities.
10
Propidium iodide by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Macao, Canada, Spain, India, Belgium, Australia, Israel, Switzerland, Poland, Ireland, Argentina, Austria, Brazil, Sweden, Portugal, New Zealand, Netherlands, Slovakia, Norway, Hungary, Czechia, Denmark
Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.

More about "Psidium guajava"

Psidium guajava, commonly known as the guava, is a small tree or shrub that is native to tropical America.
It is a widely cultivated fruit crop that is valued for its edible fruit and various medicinal properties.
The guava plant has been extensively researched, with studies exploring its phytochemistry, pharmacological activities, and potential therapeutic applications.
One of the key tools used in Psidium guajava research is the Guava flow cytometer, a compact and user-friendly instrument that allows for the analysis of cells and cellular components.
The Guava easyCyte and Guava easyCyte HT models are particularly popular among researchers, offering high-performance flow cytometry capabilities.
These instruments can be used to analyze a wide range of cellular parameters, such as cell cycle, apoptosis, and cell surface markers, all of which are relevant to understanding the mechanisms of action of guava-derived compounds.
In addition to the flow cytometers, researchers often utilize the Guava Nexin Reagent and Guava Cell Cycle reagent to stain cells for specific analyses.
The InCyte software is commonly used in conjunction with the Guava instruments to facilitate data acquisition, analysis, and reporting.
The Guava easyCyte 8HT flow cytometer is a high-throughput model that can streamline Psidium guajava research by allowing for the rapid analysis of multiple samples.
This can be particularly useful when conducting screening studies or evaluating the effects of different guava-derived compounds on cellular processes.
Overall, the integration of these advanced technologies, such as the Guava flow cytometers and associated reagents and software, can greatly enhance the optimization and reproducibility of Psidium guajava research, leading to a deeper understanding of this important plant species and its potential therapeutic applications.
Propidium iodied is another common reagent used in flow cytometry studies to stain and analyze cellular DNA content.