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Thymus Plant

Thymus Plant: A versatile medicinal herb commonly known as thyme, the Thymus plant genus is a member of the mint family.
These aromatic, evergreen perennial plants are native to the Mediterranean region and widely cultivated for their culinary and therapeutic properties.
Thymus plants are characterized by their small, fragrant leaves and clusters of tiny, pink or purple flowers.
They are valued for their essential oils, which contain compounds like thymol and carvacrol, endowing them with potent antimicrobial, antioxidant, and anti-inflammatory effects.
Thymus plants have a long history of use in traditional medicine, and their extracts are studied for potential applications in respiratory, digestive, and skin health.
Researchers are actively investigationg the optimal methods for cultivating and extracting the medicinal compounds from Thymus plants to support reproducible, high-quality studies.
PubCompare.ai can help locate the best protocols from the literature, preprints, and patents to enhance the accuracy and reproducibility of your Thymus plant research.

Most cited protocols related to «Thymus Plant»

1
Comprehensive Genome and Transcriptome of the Elephant Shark
Cited 47 times
Genomic DNA was obtained from the testis of a single C. milii caught in Tasmania, Australia, and used to prepare libraries with inserts of different sizes. Sequencing was conducted on the Roche 454 GS FLX Titanium platform (for shotgun fragments and 3-kilobase and 8-kilobase inserts) and the ABI3730 instrument (for plasmid, fosmid and BAC ends). The C. milii genome was assembled using CABOG v6.1. PolyA-selected RNA from ten tissues of C. milii (brain, gills, heart, intestine, kidney, liver, muscle, ovary, spleen and testis) and the spleen and thymus of G. cirratum were sequenced on the Illumina GAIIx platform. Transcripts were assembled de novo using Trinity r2011-07-13. MicroRNA genes were identified by deep sequencing of small RNA from 16 tissues (brain, blood, eye, gills, heart, intestine, kidney, liver, muscle, ovary, pancreas, rectal gland, skin, spleen, testis and uterus) on the Illumina GAIIx platform. Annotation of the genome was carried out using the Ensembl gene annotation pipeline which integrated ab initio gene predictions and evidence-based gene models. The gene set can be viewed at http://esharkgenome.imcb.a-star.edu.sg/. Annotation of protein domains in the C. milii proteome was carried out by searching it against the Pfam v26 database using HMMER v3.0. Zebrafish spp1 was knocked down using morpholinos or the CRISPR/Cas9 system. Methods are described in detail in individual sections of the Supplementary Information.
All animals were cared for in strict accordance with National Institutes of Health (USA) guidelines. The protocol was approved by the Institutional Animal Care and Use Committee of the Biological Resource Centre, A*STAR (protocol #100520).
Venkatesh B., Lee A.P., Ravi V., Maurya A.K., Lian M.M., Swann J.B., Ohta Y., Flajnik M.F., Sutoh Y., Kasahara M., Hoon S., Gangu V., Roy S.W., Irimia M., Korzh V., Kondrychyn I., Lim Z.W., Tay B.H., Tohari S., Kong K.W., Ho S., Lorente-Galdos B., Quilez J., Marques-Bonet T., Raney B.J., Ingham P.W., Tay A., Hillier L.W., Minx P., Boehm T., Wilson R.K., Brenner S, & Warren W.C. (2014). Elephant shark genome provides unique insights into gnathostome evolution. Nature, 505(7482), 174-179.
Publication 2014
Animals Biopharmaceuticals BLOOD Brain Clustered Regularly Interspaced Short Palindromic Repeats Gene Annotation Genes Genome Gills Heart Institutional Animal Care and Use Committees Intestines Kidney Liver MicroRNAs Morpholinos Muscle Tissue Ovary Pancreas Plasmids Protein Annotation Proteome RNA, Polyadenylated Salt Gland Skin Spleen SPP1 protein, human Testis Thymus Plant Tissues Titanium Uterus Zebrafish
2
Comprehensive Profiling of Human Transcripts
Cited 22 times
Similar amounts of 24 human cDNAs (brain, heart, kidney, spleen, liver, colon, small intestine, muscle, lung, stomach, testis, placenta, skin, PBLs, bone marrow, fetal brain, fetal liver, fetal kidney, fetal heart, fetal lung, thymus, pancreas, mammary glands, prostate; final dilution 1,000×) were mixed with JumpStart REDTaq ReadyMix (Sigma, St Louis, MO, USA) and 4 ng/μl primers (Sigma-Genosys, St Louis, MO, USA)) with a BioMek 2000 robot (Beckman, Fullerton, CA, USA) as described and modified [43 (link)-45 (link)]. The 10 first cycles of PCR amplification were performed with a touchdown annealing temperature decreasing from 60°C to 50°C; the annealing temperature of the next 30 cycles was 50°C. Amplimers were separated on 'Ready to Run' precast gels (Pfizer, New York, NY, USA) and sequenced. This procedure was used to experimentally assay 1,215 exon-exon junctions of human genes predicted by five ab initio and four EST-based methods outside of HAVANA objects and 83 HAVANA novel and 78 putative transcripts (see Results and discussion for details).
Harrow J., Denoeud F., Frankish A., Reymond A., Chen C.K., Chrast J., Lagarde J., Gilbert J.G., Storey R., Swarbreck D., Rossier C., Ucla C., Hubbard T., Antonarakis S.E, & Guigo R. (2006). GENCODE: producing a reference annotation for ENCODE. Genome Biology, 7(Suppl 1), S4.
Publication 2006
Biological Assay Bone Marrow Brain Care, Prenatal Colon DNA, Complementary Exons Fetal Heart Gels Heart Homo sapiens Intestines, Small Kidney Liver Lung Mammary Gland Muscle Tissue Oligonucleotide Primers Pancreas Placenta Prostate Reverse Transcriptase Polymerase Chain Reaction Skin Spleen Stomach Technique, Dilution Testis Thymus Plant
3
Porcine Tissue RNA Extraction Protocols
Cited 20 times
Seventeen different porcine tissues were collected from three young female siblings. Total RNA was extracted using different protocols depending of the tissue: TRIreagent® (Molecular Research Centre, inc.) for liver, kidney, thymus, RNeasy lipid kit (Qiagen) for adipose (subcutaneous), cortex cerebri, cerebellum, hippocampus, lymph nodules (jejunal), RNeasy Fibrous Kit (Qiagen) for muscle (longissimus dorsi), heart (muscle), skin (dermis and epidermis) and RNeasy kit (Qiagen) for pancreas, bone marrow, bladder, lung, stomach (mucosal membranes), small intestine (mucosal membranes) according to each manufacturer protocol. Contaminating DNA was degraded by treating each sample with RQ1 RNase-free DNase (Promega) according to the instructions manual, followed by a spin-column purification (Qiagen RNeasy). The total RNA was quantified by optical density and the quality was evaluated by gel electrophoresis. Intact rRNA subunit of 28S and 18S were observed on the gel indicating minimal degradation of the RNA.
Nygard A.B., Jørgensen C.B., Cirera S, & Fredholm M. (2007). Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR. BMC Molecular Biology, 8, 67.
Publication 2007
Bone Marrow Cerebellum Cortex, Cerebral Deoxyribonucleases Dermis Electrophoresis Endoribonucleases Epidermis Females Fibrosis Intestines, Small Jejunum Kidney Lipids Liver Lung Mucous Membrane Muscle Tissue Myocardium Nodes, Lymph Obesity Pancreas Pigs Promega Protein Subunits RNA, Ribosomal, 28S RNA Degradation Seahorses Sibling Skin Stomach Thymus Plant Tissue, Membrane Tissues Urinary Bladder Vision
4
Isolation and Analysis of Mouse Lymphocytes
Cited 18 times

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Publication 2015
alpha HML-1 Antibodies Bicarbonates BLOOD Buffers CD44 protein, human Cells Cervix Uteri Collagenase, Clostridium histolyticum Dithioerythritol Enzymes Female Reproductive System Flow Cytometry Hemoglobin, Sickle HEPES Hyperostosis, Diffuse Idiopathic Skeletal Intestines Intestines, Small isolation Kidney Lamina Propria Large Intestine Liver Lung Lymphocyte Matrix Metalloproteinase 2 Mucus Mus Needles Nodes, Lymph Nylons Pancreas Passive Immunization Percoll Polystyrenes Salivary Glands Spleen Stomach Streptavidin Syringes Thymus Plant Tissues Uterine Cornua Vagina
5
Identifying Regulatory Elements via Combinatorial ATAC-seq
Cited 17 times

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Publication 2018
ATAC-Seq Brain Cells Cerebellum Deoxyribonuclease I Dietary Fiber DNA Replication Heart Intestines, Small Kidney Liver Lung Regulatory Sequences, Nucleic Acid Spleen Thymus Plant Tissues

Most recents protocols related to «Thymus Plant»

1
Standardized Anti-Nucleosome and Anti-RNA ELISAs
Anti-nucleosome ELISAs were performed by coating Immulon 2HB plates with 10 μg/ml poly-L-lysine (Sigma-Aldrich) in PBS. Plates were washed and coated with 15 μg/ml dsDNA prepared by digestion of calf thymus DNA (Sigma-Aldrich) with S1 nuclease (Promega) for 30 min at 37 deg followed by ethanol precipitation. Plates were subsequently washed and coated with 10 μg/ml calf thymus histones type IIAS (Sigma-Aldrich). Plates were blocked with ELISA buffer (1× PBS 1% BSA 0.05% sodium azide) and serum samples diluted 1:200 in the same buffer were applied to the top row and diluted threefold down the plate down to 1:5,400. Bound antibody was detected with alkaline-phosphatase conjugated goat anti-mouse IgG (Southern Biotech) or goat anti-mouse IgG2a (Southern Biotech) and developed with pNPP (Sigma-Aldrich). Autoantibody concentrations were determined relative to PL2-3 anti-nucleosome monoclonal antibody standard using DeltaSoft 2.8.11 software (Biometallics). Anti-RNA ELISAs were performed in a similar fashion, except plates were coated first with poly-L-lysine then with 15 μg/ml total yeast RNA (Sigma-Aldrich) before blocking and concentrations were determined relative to BWR4 standard. Total IgG and IgM ELISAs were performed by coating plates with unconjugated goat anti-mouse IgG or IgM antibody (Southern Biotech), followed by serum sample diluted 1:10,000 (IgM) or 1:50,000 (IgG) in the top row, followed by goat anti-mouse IgG-AP or IgM-AP (Southern Biotech), relative to purified IgG or IgM standards.
Nickerson K.M., Smita S., Hoehn K.B., Marinov A.D., Thomas K.B., Kos J.T., Yang Y., Bastacky S.I., Watson C.T., Kleinstein S.H, & Shlomchik M.J. (2023). Age-associated B cells are heterogeneous and dynamic drivers of autoimmunity in mice. The Journal of Experimental Medicine, 220(5), e20221346.
Publication 2023
4-aminophenylphosphate Alkaline Phosphatase anti-IgG Autoantibodies Buffers calf thymus DNA Digestion DNA, Double-Stranded Enzyme-Linked Immunosorbent Assay Ethanol Goat Histones IgG2A Immunoglobulin M Immunoglobulins Lysine Mice, House Monoclonal Antibodies Nucleosomes Poly A Promega Serum Sodium Azide Thymus Plant Yeast, Dried
2
Broiler Growth and Immune Response

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Publication 2023
Animals Animals, Laboratory Aves BLOOD Body Weight Feed Intake Heparin Liver Plasma Spleen Synovial Bursa Thymus Plant Venipuncture
3
Immune Organ Index Measurement
On the 21st day of the test, the mice were sacrificed under ether anesthesia after 6 h of LPS treatment and dissected. The thymus, spleen, and liver tissues were collected, rinsed with PBS solution, blotted dry using filter paper, and weighed. Then the immune organ index of the mice was calculated. The whole colon was taken, and the length of the colon was measured. The ileum and colon tissues and their contents were collected to detect related indicators. A part of the intestinal tissue was placed in 4% paraformaldehyde for histopathological analysis. Then, the rest of the intestinal tissue and contents were stored at −80°C.
Immune organ index = weight of organ (mg)/body weight (g).
Li X., Gui R., Wang X., Ning E., Zhang L., Fan Y., Chen L., Yu L., Zhu J., Li Z., Wei L., Wang W., Li Z., Wei Y, & Wang X. (2023). Oligosaccharides isolated from Rehmannia glutinosa protect LPS-induced intestinal inflammation and barrier injury in mice. Frontiers in Nutrition, 10, 1139006.
Publication 2023
Aftercare Anesthesia Body Weight Colon Ethyl Ether Ileum Intestines Liver Mus paraform Spleen Strains Thymus Plant Tissues
4
Organ Index and Inhibition Rate Calculation
The mice were sacrificed on the 7th day and lungs (Cui et al. 2022 ), and spleens and thymus were collected (Du et al. 2020 (link)). After washing in saline and dried, the samples were weighted, and the following formulae were used for index calculation: Organindexmg/g=Organweightmgweightg Organindexinhibitionrate%=Organindexofinfectedgroup-OrganindexofdrugtreatmentgroupOrganindexoftheinfectedgroup×100%
Sun Y., Yu C.L., Yan Y.L., Zhang F.L., Chen J., Hu Z.Y., He J., Meng X.Y, & Wu Q.F. (2023). Inhibitory Effects and Related Molecular Mechanisms of Huanglian-Ganjiang Combination Against H1N1 Influenza Virus. Revista Brasileira De Farmacognosia, 33(3), 514-522.
Publication 2023
Lung Mus Saline Solution Thymus Plant
5
Quantification of Carvacrol and Thymol in Herbs
Stock standard solutions of carvacrol and thymol (1000 mg L−1) and I.S. (100 mg L−1) were prepared in methanol. Working solutions were obtained via appropriate dilution of the stock standard solution with double distilled water. The solutions were stored in a suitable container and, then, kept at refrigerator (4 °C) in the dark place. Calibration standards were made at different concentration ranges, each one having three replicates. Furthermore, two plant materials (thymus, savory) were provided from Ilam Province, Iran. After identification, the samples were cleaned, air-dried and, finely, grounded. Subsequently, the extract was prepared via successive maceration of the powder. As follows, 5 mL of MeOH/water (80 : 20%) mixture was added to 0.2 g of samples. The mixture was sonicated for 20 min and, then, left in room temperature for 48 h. Afterwards, the mixture was centrifuged and filtered and, then, the supernatant was separated and stored at 4 °C for subsequent experiments.
Siahkamari S, & Daneshfar A. (2023). Synthesis of a new magnetic metal organic framework based on nickel for extraction of carvacrol and thymol in thymus and savory samples and analyzed with gas chromatography. RSC Advances, 13(11), 7664-7672.
Publication 2023
carvacrol Methanol Plants Powder Savory Technique, Dilution Thymol Thymus Plant

Top products related to «Thymus Plant»

1
Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
2
Trizol by Thermo Fisher Scientific
Sourced in United States, Germany, China, Japan, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Belgium, Denmark, Netherlands, India, Ireland, Lithuania, Singapore, Sweden, Norway, Austria, Brazil, Argentina, Hungary, Sao Tome and Principe, New Zealand, Hong Kong, Cameroon, Philippines
TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
3
Dnase 1 by Roche
Sourced in United States, Switzerland, Germany, Japan, United Kingdom, France, Canada, Italy, Macao, China, Australia, Belgium, Israel, Sweden, Spain, Austria
DNase I is a lab equipment product that serves as an enzyme used for cleaving DNA molecules. It functions by catalyzing the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, effectively breaking down DNA strands.
4
Rneasy mini kit by Qiagen
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
5
Calf thymus dsdna by Merck Group
Sourced in United States
Calf thymus dsDNA is a laboratory reagent composed of double-stranded DNA isolated from calf thymus tissue. It is commonly used as a standard or control in various molecular biology and biochemical applications.
6
Calf thymus histone by Merck Group
Sourced in United States
Calf thymus histones are a type of laboratory equipment used for various research and experimental purposes. Histones are a class of proteins found in the nuclei of eukaryotic cells, which play a crucial role in the organization and compaction of DNA within the cell's chromatin structure. The calf thymus histones are a commonly used source of these proteins for scientific investigations and applications.
7
Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
8
Calf thymus dna by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, Switzerland, Sao Tome and Principe
Calf thymus DNA is a purified, high-molecular-weight DNA extracted from the thymus gland of calves. It is a common laboratory reagent used as a model for studying DNA structure, interactions, and various applications in molecular biology research.
9
Nanodrop 2000 by Thermo Fisher Scientific
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The NanoDrop 2000 is a spectrophotometer designed for the analysis of small volume samples. It enables rapid and accurate quantification of proteins, DNA, and RNA by measuring the absorbance of the sample. The NanoDrop 2000 utilizes a patented sample retention system that requires only 1-2 microliters of sample for each measurement.
10
Agilent 2100 bioanalyzer by Agilent Technologies
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The Agilent 2100 Bioanalyzer is a lab instrument that provides automated analysis of DNA, RNA, and protein samples. It uses microfluidic technology to separate and detect these biomolecules with high sensitivity and resolution.

More about "Thymus Plant"

Thymus, a member of the mint family, is a versatile medicinal herb commonly known as thyme.
These aromatic, evergreen perennial plants are native to the Mediterranean region and widely cultivated for their culinary and therapeutic properties.
The Thymus genus is characterized by its small, fragrant leaves and clusters of tiny, pink or purple flowers.
These plants are valued for their essential oils, which contain compounds like thymol and carvacrol, endowing them with potent antimicrobial, antioxidant, and anti-inflammatory effects.
Thymus plants have a long history of use in traditional medicine, and their extracts are studied for potential applications in respiratory, digestive, and skin health.
Researchers are actively investigating the optimal methods for cultivating and extracting the medicinal compounds from Thymus plants to support reproducible, high-quality studies.
This includes techniques like using TRIzol reagent, TRIzol, DNase I, and the RNeasy Mini Kit to isolate and purify RNA, as well as utilizing calf thymus dsDNA, calf thymus histones, and bovine serum albumin as reference materials.
To enhance the accuracy and reproducibility of Thymus plant research, tools like NanoDrop 2000 and Agilent 2100 Bioanalyzer can be employed to assess the quality and quantity of extracted nucleic acids.
PubCompare.ai can help researchers locate the best protocols from the literature, preprints, and patents, ensuring they have access to the most effective methods for their Thymus plant experiments and studies.
By leveraging these resources, scientists can unlock the full potential of this versatile medicinal herb and advance our understanding of its therapeutic applications.