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Lizards

Lizards are a diverse group of reptiles characterized by their scaly skin, four limbs, and ability to regenerate lost body parts.
They range in size from the tiny gecko to the large Komodo dragon.
Lizards are found on every continent except Antarctica and inhabit a variety of environments, from deserts to forests.
They play important roles in their ecosystems as predators and prey.
Liszards are known for their ability to change color, run on water, and in some cases, even fly.
Their fascinating behaviors and unique adaptations have made them a popular subject of scientific study and fascination for nature enthusiasts.

Most cited protocols related to «Lizards»

Our initial squamate classification is based on the June 2009 version of the Reptile Database [1 ] (http://www.reptile-database.org/), accessed in September of 2009 when this research was begun. Minor modifications to this scheme were made, primarily to update changes in colubroid snake taxonomy [41 (link)-44 (link),205 ]. This initial taxonomic database consists of 8650 species (169 amphisbaenians, 5270 lizards, 3209 snakes, and 2 tuataras), against which the classification of species in the molecular sequence database was fixed. While modifications and updates (i.e. new species, revisions) have been made to squamate taxonomy subsequently, these are minor and should have no impact on our phylogenetic results. This database represents ~92% of the current estimated diversity of squamates (~9400 species as of December 2012).
Throughout the paper, we refer to the updated version of squamate taxonomy from the December 2012 update of the Reptile Database [1 ], incorporating major, well-accepted changes from recent studies (summarized in [1 ]). However, for large, taxonomic groups that have recently been broken up for reasons other than resolving paraphyly or matters of priority (e.g. in dactyloid and scincid lizards; see Results), we generally retain the older, more inclusive name in the interest of clarity, while providing references to the recent revision. We attempt to alter existing classifications as little as possible (see also [113 (link)]). Therefore, we generally only make changes when there is strong support for non-monophyly of currently recognized taxa and our proposed changes yield strongly supported monophyletic groups. Similarly, we only erect new taxa if they are strongly supported. Finally, although numerous genera are identified as being non-monophyletic in our tree, we refrain from changing genus-level taxonomy, given that our taxon sampling within many genera is limited.
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Publication 2013
Inclusion Bodies Lizards Reptiles Snakes Trees
We used species trees from a recent *BEAST analysis of the distichus species group in the lizard genus Anolis. Geneva et al. (2015b) (link) made species trees available (Geneva et al. 2015a ). They sampled 54 individuals from the brevirostris (8) and distichus (46) complexes, both within the distichus species group. For each individual, they sequenced DNA from seven exonic nuclear loci and from one mitochondrial locus. They used gene trees to identify putative species and generated species trees in *BEAST (Heled and Drummond 2010 (link)) using four independent analyses, each with 2 billion generations.
We sampled 1,000 trees uniformly at random from the latter half of the available *BEAST posterior (files Anoles_StarBEAST_posterior.species.trees and Anoles_StarBEAST_MCC.species.tre for the posterior trees and MCC tree, respectively, from Geneva et al. [2015a] ). We computed all pairwise tree distances according to our metric (λ  = 0) in this sample of 1,000 posterior trees. To detect clusters we used k-means clustering (using the function find.clusters from adegenet, which calls kmeans from the stats package in R [R Development Core Team 2008] ), and compared clustering solutions with the BIC, as described in the adegenet package in R (Jombart 2008 (link)). We found that a choice of k  = 8 clusters minimized the BIC. We visualized the distances using MDS (dudi.pco in the ade4 package in Chessel et al. [2004] ). Each point represents a tree, and the distances between the points approximate the distances in our metric. We colored points according to their k-means cluster. An MCC tree was found for the whole posterior and for each cluster using TreeAnnotator (Drummond and Rambaut 2007 (link)) and plotted with FigTree (Rambaut 2006 ).
We also tested a variety of other clustering methods including ward.D, ward.D2, single, complete, UPGMA, WPGMA, and WPGMC from hclust in the stats package in R, where more details on each of these methods can be found in the documentation. The clustering of the trees naturally varies slightly between methods. However, the important conclusions are insensitive to the clustering method: 1) The tighter the cluster, the more similar the trees (with any variation nearer the tips) and 2) there are about six tight clusters, corresponding to the regions in figure 3 within contour lines. Note that the contour lines are a result of the MDS projection and are therefore independent of the clustering method.
Different clustering methods in tree space can now be tested easily using the function findGroves in the package treescape (Jombart et al. 2015 ), which was developed after we performed this analysis.
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Publication 2016
Exons Genes Lizards Mitochondria Trees
UCSC released a new Conservation (13 (link)) annotation track on the March 2006 (Build 36, hg18) human genome in June 2007. This track displays multiz (14 (link)) multiple alignments of 27 vertebrate species to the human genome, along with measurements of evolutionary conservation across all 28 species and a separate measurement of conservation across the placental mammal subset of species (18 organisms). Included in the track are 5 new high-quality assemblies—horse, platypus, lizard, stickleback and medaka; 6 new low-coverage mammalian genomes—bushbaby, tree shrew, guinea pig, hedgehog, common shrew and cat; 6 updated assemblies—chimp, cow, chicken, frog, fugu and zebrafish; and 10 assemblies included in the previous version of the Conservation track—rhesus, mouse, rat, rabbit, dog, armadillo, elephant, tenrec, opossum and tetraodon. In addition to the expanded species list, the new Conservation track has been enhanced to include additional filtering of pairwise alignments for each species to reduce paralogous alignments and information about the quality of aligning species sequence included in the multiple alignments downloads. A similar Conservation annotation of at least 30 species is scheduled for release on the July 2007 (Build 37, mm9) mouse assembly in the last quarter of 2007.
Publication 2007
Armadillos Biological Evolution Bush Babies Cavia Chickens Didelphidae Elephants Equus caballus Erinaceidae Eutheria Genome Genome, Human Lizards Macaca mulatta Mammals Mice, House Oryziinae Pan troglodytes Platypus, Duckbilled Rabbits Rana Shrews Sticklebacks Strains Takifugu Tenrec Tupaiidae Vertebrates Zebrafish
The study was conducted at Midreshet Ben-Gurion in the northern Negev desert, Israel (30°51′8.27″N 34°47′0.24″E) from summer 2003 until autumn 2004. The study site was a complex of guest rooms surrounded by a two-meter high wall over an area 13x150m. A dense population of the Israeli fan-toed gecko Ptyodactylus guttatus inhabited the premises [30 –32 (link)]. The Israeli fan-toed gecko is a medium-sized, insectivorous, rupicolous, scansorial lizard [6 , 45 ] Zlotkin et al. 2003 [50 (link)], Sion et al. 2020 [33 (link)] in the family Phyllodactylidae [7 (link)] common in mesic and arid parts of the Middle East (Israel, Egypt, Saudi Arabia, Oman, Palestine, Jordan, and Syria). It often inhabits cliffs or masonry walls where it can easily be observed from a distance ([48 , 47 ], 2016 [6 , 15 (link), 30 –32 (link), 46 ]).
Fifty-five geckos were hand captured, measured (morphometrics) and scanned (DXA) and released at the site of capture. Of these, 30 gecko’s scan data were included in the comparison, since their body mass was above the lowest possible accurate reading with minimal body mass (> 4.8 g) as indicated in the results (Table 1). The snout vent length (SVL) of these 30 geckos was 60.6–91.7 mm and their body mass 4.99–22.5 g. We used these 30 geckos to compare the real wet mass (as measured by a scale) and the wet mass measure by DXA (see below). From each captured gecko, we recorded the mass using Ohaus digital scale to 0.1 g precision, snout-vent length (SVL), using digital calipers, and the width at the base of the tail. Six additional individuals were captured and euthanized for the calibration necessary for this study (two males, three females and one too small to be sexed without a probe) The smallest gecko (55.6 mm) with body mass 4.2 g was excluded to improve accuracy from 55 to 8.5% error. The snout vent length (SVL) of these geckos was 61.5–91.7 mm and their body mass 4.8–11.5 g. We killed only six geckos in order to minimize destructive sampling as much as possible.

Sex, snout-vent length (SVL), live wet body mass, DXA mass reading and fat mass chemical extraction of six Israeli fan-toed geckos Ptyodactylus guttatus used to validate the application of dual-energy X-ray absorptiometry (DXA) to non-invasively calculate body fat indices in small lizards

SexSVL (mm)Live Wet Mass (g)DXA Mass Reading (g)Fat Extraction (g)DXA Fat Reading (g)
Male61.514.85.70.261.5
Male87.4511120.371.65
Female80.7410.911.91.602.4
Female65.9711.5132.092.8
Female67.146.57.60.932
Unknowna55.644.25.20.430.95

a Indicates the lizard that was removed from the validation experiment (see text for details)

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Publication 2021
Body Fat Females Fingers Geckos Human Body Insectivora Lizards Males Palestinians Radionuclide Imaging Tail Thumb
The ambon damselfish Pomacentrus amboinensis and lemon damsel P. moluccensis are common site attached species of damselfish (family Pomacentridae) found throughout the Indo-Pacific on shallow reef habitats at the interface between the live coral and rubble reef edge (Fig. S1). Both species have a similar larval duration after a demersal egg phase and settle at similar sizes (P. amboinensis 17.8 d, 11.2 mm SL; P. moluccensis 19.4 d, 10.7 mm SL; [17] ). Metamorphosis is concomitant with settlement and in these species involves a major change in pigmentation (transparent to coloured) that occurs within hours, but involves little obvious change in shape [36] . However, settlement does involve major changes in physiology [37] and it is likely that marked changes also occur in the sensory systems [38] . A laboratory-based habitat selection experiment has previously shown that both species preferentially settle to healthy live coral [39] . Both species settle naturally to patches of mixed live and dead coral. Both are also planktivores as juveniles and eat a similar array of prey items (Text S1). A tagging study of 295 newly settled P. amboinensis on the continuous reef edge found that fish moved little over the first 3 months after settlement (mean = 0.63 m [40] ). It is likely that P. moluccensis has a similar degree of site attachment (pers. obs.).
Research on newly settled P. amboinensis has shown that fish enter the reef with high variability in their behavioural traits (e.g. boldness, aggression) and these traits are displayed in a manner that is consistent on small time scales of hours to days ([41] (link), [42] (link), Mero, Meekan and McCormick unpublished data]. Establishment of dominance hierarchies occurs within minutes of settlement within the species, which can rapidly lead to the eviction of subordinates from small habitat patches [31] (link). Because of the rapid establishment of territories and the high juvenile mortality, it was decided that 60 min was an ecologically relevant time to use for the establishment of residents in the priority experiments for the present study.
The present datasets were collected at Lizard Island (14° 40′S 145° 28′ E) on the northern Great Barrier Reef, Australia, between October 2007 and March 2010. Both newly metamorphosed juveniles and recently settled juveniles from the reef were used for field experiments. Light traps (see [43] for design; small trap) were used to collect both fish species at the end of their larval phase prior to their settlement to the reef. These newly metamorphosed fish were separated by species and placed into 60 L aquaria with aerated flowing seawater. Fish were kept for 24 h and fed newly hatched Artemia sp. twice per day ad libitum to allow recovery from (or acclimation to) the stress of capture, prior to use in experiments. Juveniles were collected from a shallow fringing reef at the back of Lizard Island using a solution of dilute clove oil and hand nets. All fishes used in the experiments were placed into a small clip-seal bag with a small amount of aerated seawater and measured with calipers (±0.1 mm) and then transferred into individually labeled 1 L clip-seal bags for transport. To reduce transport and handling stress, fish in bags were transported to the field site in a 30 L bin of seawater (to reduce temperature fluctuations) under subdued light conditions.
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Publication 2012
Acclimatization Artemia BAG1 protein, human Biological Metamorphosis Citrus limon Clip Coral Fishes Larva Light Lizards Oil, Clove Phocidae physiology Pigmentation Sensory System SLC6A2 protein, human

Most recents protocols related to «Lizards»

Thirty-six liver samples from the G.galloti lizards previously studied by Anettová et al. [2 (link)] were used in the present investigation. Briefly, specimens of G.galloti were captured in Tegueste (Tenerife, Canary Islands, Spain) and euthanised at the Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC). Liver samples were obtained during dissection and preserved in absolute ethanol for molecular analysis. DNA was isolated from the tissue samples using the Qiagen DNEasy Blood & Tissue Kit (Hilden, Germany) with the following modifications: 25 μl of proteinase K and lyse phase extended to overnight.
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Publication 2023
BLOOD Dissection Endopeptidase K Ethanol Liver Lizards Tissues
Two types of immunofluorescence detections were performed on the BrdU-treated lizards. For those in the first group, with survival times of 1.5 h or 3 days (n = 3 each), a triple immunofluorescence detection for GFAP/DCX/BrdU was performed. For those in the second group, with a survival time of 7 days from the first injection and 3 days from the last injection (n = 3), a double immunofluorescence for BrdU/PCNA was performed.
In both cases the protocol for fluorescence immunohistochemistry was similar, except for the antibodies used. First, the slides were deparaffinized and hydrated. Then, they were treated with HCl 2N for 10 min at 37°C for DNA denaturation, rinsed in 0.1 M borate buffer and washed in phosphate buffered saline containing 0.1% Triton X-100 and BSA 0.1% (PTA). Subsequently, the sections were incubated in a blocking solution containing 10% casein (Vector) or 5% normal goat serum (NGS) (Sigma, San Luis, MO, USA) in PTA for 1 h, for triple or double immunoassay, respectively. After rinsing in PTA, the sections were incubated in blocking solution with the corresponding primary antibodies overnight at 4°C. The primary antibodies used for the first group were: mouse anti-BrdU (1:150, Dako), rabbit anti-GFAP (1:500, Dako), and goat anti-DCX (1:200, Sta. Cruz Biotechnologies); and for the second group: mouse anti-PCNA (1:500, Sigma, San Luis, MO, USA), and rat anti-BrdU (1:200, Abcam, Cambridge, UK). Sections were then washed with PTA and incubated with fluorescent secondary antibodies at 1:500 in blocking solution for 1 h at room temperature in the dark. The secondary antibodies used for the first group were: donkey anti-mouse Alexa 647 (1:500, Invitrogen, Walthan, MA, USA), donkey anti-rabbit Alexa 488 (1:500, Invitrogen, Walthan, MA, USA), and donkey anti-goat Alexa 555 (1:500, Invitrogen, Walthan, MA, USA); and for the second group: goat anti-mouse Alexa 555 (1:500, Invitrogen, Walthan, MA, USA), and goat anti-rat Alexa 488 (1:500, Invitrogen, Walthan, MA, USA). The sections were then washed in 0.1 M PB and incubated for 10 min with DAPI 1:1000 in H2O (Sigma, San Luis, MO, USA) at room temperature in the dark. Finally, the slides were washed with 0.1 M PB and mounted with Fluorsave (Calbiochem). The sections were analyzed with a Leica (Wetzlar, Germany) SP2 TCS AOBS inverted confocal microscope.
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Publication 2023
Antibodies Borates Bromodeoxyuridine Caseins Cloning Vectors DAPI DNA Denaturation Equus asinus Fluorescence Fluorescent Antibody Technique Glial Fibrillary Acidic Protein Goat Immunoassay Immunohistochemistry Lizards Mice, House Microscopy, Confocal Phosphates Proliferating Cell Nuclear Antigen Rabbits Saline Solution Serum Triton X-100
To assess the distribution of proliferating cells in the VZ, lizards injected with [3H]-thymidine with a survival time of 1.5 h were used (n = 5). The VZ was divided in six different regions, including three sulcal zones (sulcus medalis, sulcus lateralis and sulcus ventralis/terminalis) and three intersulcal zones (intersulcus corticalis, intersulcus lateralis, and intersulcus septalis) (Figure 1B). The number of [3H]-thymidine labeled cells was counted in all these regions relative to the total number of cells. This quantification was performed in two telencephalic levels: one pre-commissural (anterior) and one post-commissural (posterior), analyzing for each level a total of 7 semithin sections which were 9 μm apart to avoid counting the same cell twice. Different types of counts were performed by quantifying the total number of labeled cells/1000 cells considering sulci vs. intersulcal regions, comparing between the different sulci and intersulcal regions and differentiating between the pre- and post-commissural levels for each animal.
To characterize the ultrastructure of VZ proliferative cells and their derivatives, the brains of specimens with 1.5, 6, 12, 24, and 72 h survival times were examined. Between 50 and 150 [3H]-thymidine-positive ([3H]-thy+) cells were analyzed for each survival time, including at least two different antero-posterior levels per lizard. These cells were studied by transmission electron microscopy (TEM) to determine their ultrastructural characteristics. Counts were also made of the number of cells in mitosis (M phase) labeled relative to the total number of [3H]-thy+ cells.
The analysis of specimens with long survival times (1, 3, 6, and 12 months) focused mainly on the cell layer of the MC, although we also investigated whether there were labeled cells in the walls of the LVs. Within the MC we analyzed the ultrastructure of 25–50 [3H]-thy+ cells from each survival time to see to which neuronal type they corresponded.
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Publication 2023
Animals Brain Cells Cell Survival Cell Wall derivatives Division Phase, Cell Lizards Mitosis Neurons Telencephalon Thymidine Transmission Electron Microscopy
Lizards received intraperitoneal injections of [3H]-thymidine (Amersham; specific activity 5 Ci/mmol). Depending on their survival time, the final dose was different. For survival times of 1.5 h to 3 days, the animals received a single injection with a dose of 5 μCi/g body weight (b. wt.), while for longer survival times (1–12 months) animals received daily injections (5 μCi/g b. wt.) during three consecutive days, up to a total dose of 15 μCi/g b. wt.
For short survival times, we injected four lizards (n = 4) for each survival time (6, 12, 24, and 72 h), except for the 1.5 h survival time, for which we injected five (n = 5). For long survival times (1, 3, 6, and 12 months) we injected 3 animals for each time (n = 3).
Following their corresponding survival time, the animals were deeply anesthetized with Ketolar (ketamine hydrochloride, 0.6 mg/g b. wt.) and perfused with saline (0.9% NaCl), followed by a fixative consisting of 4% PFA and 2% GA. The complete bodies of the lizards were postfixed in the same fixative during 24 h. The brains were removed from the skull, sectioned frontally or longitudinally on a vibratome at 200 μm, postfixed in 2% osmium tetroxide for 2 h, rinsed, dehydrated, and embedded in epoxy resin (Durcupan, Sigma, San Luis, MO, USA). Semithin sections were cut at 1.5 μm with an ultramicrotome (UC6 Ultracut, Leica, Wetzlar, Germany) and mounted on gelatin-coated glass-slides, which were dipped in LM-1 hypercoat emulsion (Amersham), dried in the dark, and stored at 4°C for 30 days. The autoradiographs were developed using standard methods and counterstained with 1% toluidine blue.
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Publication 2023
Animals Autoradiography Body Weight Brain Cranium Desiccation Durcupan Emulsions Epoxy Resins Fixatives Gelatins Human Body Injections, Intraperitoneal Ketamine Hydrochloride Lizards Normal Saline Osmium Tetroxide Saline Solution Thymidine Tolonium Chloride Ultramicrotomy
For this experiment, a total of 10 adult lizards of both sexes were used. Five animals underwent a complete olfactory peduncle sectioning procedure (n = 5) while the rest of them (n = 5) were subjected to a sham surgery. In both cases the animals were anesthetized with ketamine hydrochloride (Ketolar) at a concentration of 375 μg/g b. wt. The two frontoparietal scales, located between the supraocular scales and the pineal eye, were lifted, the skull was pierced centrally, and the meninges were gently removed. In one of the groups, the olfactory peduncle was sectioned bilaterally and Gelfoam (Pfizer, New York, NY, USA) applied between the resulting ends, while in the other (control group) Gelfoam was placed on top of the olfactory peduncles without sectioning them. Then, the skull window was closed using bone wax.
Animals were maintained for 2 weeks after surgery to allow them to recover. Then they were injected with 5 μCi/g b. wt. of [3H]-thymidine for 3 consecutive days, receiving a total dose of 15 μCi/g b. wt. Lizards were allowed 1 month of survival after [3H]-thymidine administration.
One of the OBs from each specimen was processed for electron microscopy and embedded in epoxy resin as described above. From a randomly selected starting level, 30 semithin sections (1.5 μm thick) were obtained and analyzed, corresponding to 45 μm. Following this procedure, successive series obtained every 200 μm were studied to cover the whole OB. Autoradiographic detection was performed on these sections as described in the corresponding section.
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Publication 2023
Adult Animals bone wax Cranium Electron Microscopy Epoxy Resins Gelfoam Ketamine Hydrochloride Lizards Meninges Operative Surgical Procedures Peduncle, Olfactory Pineal Gland Thymidine

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More about "Lizards"

Reptilians, Saurians, Lacertilia, Squamata, Gekkonidae, Agamidae, Iguanidae, Chamaeleonidae, Varanidae, Cordylidae, Scincidae, Teiidae.
Lizards are a diverse group of reptiles found on every continent except Antarctica, ranging in size from tiny geckos to the massive Komodo dragon.
These fascinating creatures are known for their scaly skin, four limbs, and remarkable ability to regenerate lost body parts.
Lizards play crucial roles in their ecosystems as both predators and prey, and their unique adaptations, such as color-changing, water-walking, and even gliding capabilities, have made them a popular subject of scientific study and nature enthusiasts' fascination.
Cutting-edge tools like the DNeasy Blood and Tissue Kit, HiSeq 2000 and HiSeq 2500 sequencers, and genetic models like C57BL/6J, 129X1/SvJ, Rpl22tm1.1Psam, Tg(Ddx4-cre)1Dcas, and Tg(Neurog3-cre)C1Able/J, have revolutionized lizard research, allowing scientists to delve deeper into the mysteries of these remarkable reptiles.
Whether you're a researcher, nature enthusiast, or simply curious about the wonders of the natural world, the insights and tools available through PubCompare.ai can help you unlock the secrets of the lizard kingdom and take your studies to new heights.