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Cytomegalovirus

Cytomegalovirus (CMV) is a common virus that belongs to the herpesvirus family.
It is a leading cause of congenital infections, often causing lifelong latent infections in humans.
CMV can cause severe disease in immunocompromised individuals, such as those with HIV/AIDS or organ transplant recipients.
Reseachers can optimize their CMV studies using PubCompare.ai, an AI-powered platform that helps identify the best protocols from literature, preprints, and patents.
Its cutting-edga comparison tools allow users to find the most reproducible and effective approaches, boosting research efficieny and productivity.
Expirience the power of AI-driven protocol comparison today.

Most cited protocols related to «Cytomegalovirus»

1
TALEN-Mediated Gene Editing Protocols
One of the pairs of TALENs targeting the human HPRT1 gene was subcloned into the mammalian expression vector pCDNA3.1(-) (Invitrogen) using XhoI and AflII. These enzymes excise the entire TALEN from pTAL3 or pTAL4 and place the coding sequence under control of the CMV (cytomegalovirus) promoter. The resulting plasmids were introduced into HEK293T cells by transfection using Lipofectamine 2000 (Invitrogen) following the manufacturer's protocol. Cells were collected 72 h after transfection and genomic DNA isolated and digested with Hpy188I, which cuts in the spacer sequence of the TALEN target site. After digestion, a chromosomal fragment encompassing the target site was amplified by PCR. Upon completion, the reactions were incubated for 20 min at 72°C with 4 µl of Taq DNA polymerase. PCR products then were digested with Hpy188I and cloned in a TOPO TA vector (Invitrogen). Independent clones containing the full-length PCR product were sequenced to evaluate mutations at the cleavage site.
The TALENs targeting the Arabidopsis ADH1 gene were subcloned into the plant expression vector pFZ14 (27 (link)) using XbaI and SacI. These enzymes excise the entire TALEN from pTAL3 or pTAL4 and place the coding sequence under control of the CaMV (cauliflower mosaic virus) 35S promoter. Recombinant plasmids were transformed into Arabidopsis protoplasts as previously described (27 (link)). Forty-eight hours after transformation, DNA was prepared and digested with PflFI, which cuts in the spacer sequence of the TALEN target site. After digestion, a chromosomal fragment encompassing the target site was amplified by PCR and the reaction products were once again digested with PflFI and run on an agarose gel. The band corresponding in size to undigested product was excised and cloned and individual clones were sequenced to evaluate mutations at the cleavage site.
Cermak T., Doyle E.L., Christian M., Wang L., Zhang Y., Schmidt C., Baller J.A., Somia N.V., Bogdanove A.J, & Voytas D.F. (2011). Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 39(12), e82.
Publication 2011
Arabidopsis Cauliflower Mosaic Virus Cells Chromosomes Clone Cells Cloning Vectors Cytokinesis Cytomegalovirus Digestion DNA polymerase C Enzymes Genes Genome lipofectamine 2000 Mammals Mutation Open Reading Frames Plants Plasmids Protoplasts Sepharose Topotecan Transcription Activator-Like Effector Nucleases Transfection
2
Generation and Characterization of Ins1-Cre and Ins1-CreERT2 Mice

Mice Ins1Cre (Ins1tm1(cre)Thor) and Ins1CreERT2 (Ins1tm1(CreERT2)Thor) knock-in mice were generated by Genoway (Lyon, France) and kept on a C57Bl/6J genetic background. Briefly, a targeting vector was created by inserting the Cre or CreERT2 recombinase genes by homologous recombination in the second exon of the Ins1 gene so that the coding region of the recombinase starts at the initiation codon and replaces the Ins1 coding sequence (Fig. 1). Following transfection of the targeting vector into embryonic stem cells, negative and positive selections were performed by diphtheria toxin and neomycin (neo) selection, respectively. Cells with confirmed homologous recombination were used to generate chimeric mice. These were crossed with C57Bl/6J mice, and mice with germline transmission were crossed with C57Bl/6J flippase (Flp) deleter mice to eliminate the neo cassette. Mice with germline transmission of the recombined allele and deletion of the neo cassette were then crossed with Rosa26-eYFP mice [20 (link)] or Rosa26-tdTomato mice [21 (link)]. Animals were housed four to five per cage at 23°C on a 12 h light/dark cycle and were fed a standard rodent chow (Diet 3436; Provimi Kliba, Penthallaz, Switzerland). To induce recombination in Ins1CreERT2 crossed with Rosa26 reporter mice, tamoxifen (T5648; Sigma-Aldrich, St Louis, MO, USA) was dissolved in corn oil at 20 mg/ml, and 2 mg was injected subcutaneously four times over a 2 week period.

Generation of Ins1Cre and Ins1CreERT2 mice. (a) Structure of the Ins1 locus, of the targeting vector, of the targeted allele, and of the Ins1Cre locus following flipase (Flp)-dependent removal of the flipase recognition site (Frt)-flanked neo cassette. DTA, diphtheria toxin receptor gene; Ex, exon; ATG, translation initiation codon. (b) The Ins1CreERT2 allele was obtained using the targeting strategy depicted in (a). (c) Structure of the Rosa26-eYFP/tdTomato transgene and localisation of the primers (arrows) used for PCR analysis of the recombined locus. CMV/A, cytomegalovirus/actin promoter; (d) PCR analysis shows recombination in the pancreas but not in the liver, cortex, hypothalamus or cerebellum of Ins1Cre/+;Rosa26-eYFP. No recombination was observed in the pancreas of Rosa26-eYFP littermates; amplification of the Gapdh gene is used as a control

Thorens B., Tarussio D., Maestro M.A., Rovira M., Heikkilä E, & Ferrer J. (2014). Ins1Cre knock-in mice for beta cell-specific gene recombination. Diabetologia, 58(3), 558-565.
Publication 2014
Actins Alleles Allogeneic Cells Animals Cells Cerebellum Chimera Cloning Vectors Codon, Initiator Corn oil Cortex, Cerebral Cytomegalovirus Deletion Mutation Diet Diphtheria Toxin Diphtheria Toxin Receptor Embryonic Stem Cells Exons GAPDH protein, human Gene Amplification Genes Genetic Background Genetic Vectors Germ Line Homologous Recombination Hypothalamus Liver Mice, Inbred C57BL Mice, Laboratory Neomycin Oligonucleotide Primers Open Reading Frames Pancreas Recombinase Recombination, Genetic Rodent Tamoxifen tdTomato Transgenes Transmission, Communicable Disease
3
Generating mKate2 Expression Under Xanf1 Promoter
To generate a construct expressing mKate2 under the control of Xanf1 promoter, the 2200 bp fragment of this promoter sufficient for specifically targeting the expression to the anterior neural plate [10 (link)] was subcloned into VspI (blunted)/AgeI sites of mKate2-N vector, instead of the multiple cloning site region and CMV (cytomegalovirus) promoter. For transgenic experiments, vectors were linearized by SfiI and purified using Qiagen columns. Transgenic embryos were generated by the REMI (restriction enzyme-mediated integration) technique exactly as described previously [10 (link)] and were analysed using a Leica MZFLIII fluorescent stereomicroscope with the rhodamine filter set: excitation filter 546/10 nm; suppression filter 565 nm. To visualize a fluorescent signal on tissue sections, transgenic embryos were fixed overnight in 4 % (w/v) formaldehyde in 0.1 % PBS, embedded in 3 % agarose in 0.1 % PBS and sectioned using a HB 650 Vibratome (Microme) in 50 μm sections. The animal work was performed in Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, in accordance with the regulations of the Department of Health and Human Services, National Institutes of Health.
SHCHERBO D., MURPHY C.S., ERMAKOVA G.V., SOLOVIEVA E.A., CHEPURNYKH T.V., SHCHEGLOV A.S., VERKHUSHA V.V., PLETNEV V.Z., HAZELWOOD K.L., ROCHE P.M., LUKYANOV S., ZARAISKY A.G., DAVIDSON M.W, & CHUDAKOV D.M. (2009). Far-red fluorescent tags for protein imaging in living tissues. The Biochemical journal, 418(3), 567-574.
Publication 2009
Animals Animals, Transgenic Cloning Vectors Cytomegalovirus DNA Restriction Enzymes Embryo Formaldehyde Neural Plate Rhodamine Sepharose Tissues
4
SARS-CoV-2 specific MP stimulation protocol

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Publication 2020
Antibodies Biological Assay Cells COVID 19 Cytokine Cytomegalovirus Donors Severe acute respiratory syndrome-related coronavirus Sulfoxide, Dimethyl
5
Cloning and Transfection of STAT3 and HMGA1a Vectors
The pSG5-STAT3-C vector was made by excision of constitutively activated STAT3 (STAT3-C) from STAT3 pcDNA3.1 vector (14 (link)) with Pme1 and BamH1. The STAT3-C fragment was cloned into pSG5 after restriction with EcoR1 and Klenow treatment. The PA-HMGA1a vector was made by excision of the full-length murine HMGA1a cDNA from the vector pHEBoNeo-HMG-I3 with HindIII, Klenow treatment, and subsequent NotI digestion. The HMGA1a fragment was subsequently cloned into the EF1α expression vector (18 (link)) after restriction with BamHI and Klenow treatment. The STAT promoter vectors were described (kindly provided by K. Kohno, Department of Molecular Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan; ref. 19 (link)).
Transfections were done using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions as we previously described (10 (link)). In the experiments with HEL control or HEL-HMGA1 cells, the STAT3 promoter reporter plasmid (1 μg) was cotransfected with the control pRL-TK vector (100 ng) containing Renilla luciferase (Promega) to control for transfection efficiency (3 × 105 cells/500 μL per transfection). The DNA was mixed with Lipofectamine 2000 at a ratio of 1 μg:1.5 μL (in Opti-MEM, Invitrogen) and incubated with cells for 6 h. Cells were harvested for luciferase activity 24 h after transfection and experiments were done twice in triplicate. In the titration experiment, reporter plasmid (100 ng), control Renilla luciferase plasmid (50 ng), and 0 to 150 ng of HMGA1a expression plasmid (pSG5-HMG-I) were cotransfected as described above. The pSG5 plasmid was added (0–150 ng) to keep the total DNA constant in the titration experiments (1 × 105 cells/100 μL per transfection). The DNA was mixed with Lipofectamine 2000 at a ratio of 1 μg:1.67 μL (in Opti-MEM, Invitrogen) and cells were harvested as described above.
The lentiviral construct expressing dominant-negative STAT3 (Lenti-DN-STAT3) was made from the pcDNA3.1-STAT3DN (provided by J-I. Park, the Johns Hopkins University School of Medicine, Baltimore, MD; ref. 14 (link)). The cytomegalovirus promoter was replaced by the human EF1α promoter from pEF1/Myc-His-A (Invitrogen) and the EF-DN-STAT3 cassette was cloned into the cFUGW lentivirus (provided by D. Baltimore, Division of Biology, California Institute of Technology, Pasadena, CA) at the PacI site and lentiviral supernatants were made as described (20 (link)).
Hillion J., Dhara S., Sumter T.F., Mukherjee M., Di Cello F., Belton A., Turkson J., Jaganathan S., Cheng L., Ye Z., Jove R., Aplan P., Lin Y.W., Wertzler K., Reeves R., Elbahlouh O., Kowalski J., Bhattacharya R, & Resar L.M. (2008). The High-Mobility Group A1a/Signal Transducer and Activator of Transcription-3 Axis: An Achilles Heel for Hematopoietic Malignancies?. Cancer research, 68(24), 10121-10127.
Publication 2008
Cells Cloning Vectors Cytomegalovirus Digestion DNA, Complementary HMGA1 protein, human Homo sapiens Lentivirus lipofectamine 2000 Luciferases Luciferases, Renilla Mus Pharmaceutical Preparations Plasmids Promega STAT3 protein, human Titrimetry Transfection

Most recents protocols related to «Cytomegalovirus»

1
Evaluating Fetal Arrhythmia: Comprehensive Approach
Following a diagnosis of fetal arrhythmia, TORCH (toxoplasmosis, other [syphilis], rubella, cytomegalovirus, herpes simplex virus) and thyroid function were evaluated in maternal peripheral blood. Pregnant women with fetal arrhythmia were hospitalized so that they could be more closely observed for signs of fetal compromise or distress. Early delivery was considered and weighed against the complications of prematurity and fetal status in utero.
Hu Q., Liao H., Xu T., Liu H., Wang X, & Yu H. (2023). Perinatal outcomes of intrauterine fetal arrhythmias: A 10-year retrospective cohort study. Medicine, 102(10), e33244.
Publication 2023
BLOOD Cardiac Arrhythmia Care, Prenatal Cytomegalovirus Fetal Diagnosis Mothers Obstetric Delivery Pregnant Women Premature Birth Rubella Simplexvirus Syphilis Thyroid Gland Toxoplasmosis Uterus
2
Dual AAV Vectors for MYO7A Gene Delivery

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Publication 2023
Actins Bacteriophages Chickens Cloning Vectors Cytomegalovirus Exons Genome growth hormone, bovine Homo sapiens Hybrids Introns Open Reading Frames Photoreceptor Cells Plasmids Polyadenylation Simian virus 40 Tissue Donors Transgenes VIIa, Myosin
3
Comprehensive Adverse Event Monitoring in Clinical Trial
Adverse events were assessed according to the Common Terminology Criteria for Adverse Events, version 5.0. All patients were evaluated prior to inclusion with a medical examination by the treating physician. This included spleen palpation (no ultasonography nor computed tomography was performed), blood sample analyses (Hemoglobin, leukocyte differentiation count, platelets, IgG, IgA, IgM, Hematocrit, Bilirubin, Potassium, Sodium, Creatinine, albumin, uric acid, lactate dehydrogenase, Alkaline Phosphatase, Alanine transaminase, amylase, bilirubin, D-dimer, ionized calcium, C-Reactive Protein, Thyrotropin, thyroxin, Luteinizing Hormone, Adrenocorticotropic Hormone, Cortisol, Hepatitis B, hepatitis C (IgG), HIV, HTLV-1(IgG), IgG and IgM for Cytomegalovirus (CMV), Epstein-Barr Virus (EBV) and toxoplasmosis) and an electrocardiogram. According to the treatment plan, patients were evaluated at inclusion, during treatment pause, and at the end of the trial (EOT). At every vaccine treatment, an investigator recorded the patient’s symptoms, and when necessary, conducted a medical examination and evaluation. Bone marrow biopsies were acquired from patients before trial entry and at end of the trial. Histopathological evaluation of nonblinded biopsies was performed by a trained hematopathologist.
Grauslund J.H., Holmström M.O., Martinenaite E., Lisle T.L., Glöckner H.J., El Fassi D., Klausen U., Mortensen R.E., Jørgensen N., Kjær L., Skov V., Svane I.M., Hasselbalch H.C, & Andersen M.H. (2023). An arginase1- and PD-L1-derived peptide-based vaccine for myeloproliferative neoplasms: A first-in-man clinical trial. Frontiers in Immunology, 14, 1117466.
Publication 2023
Alanine Transaminase Albumins Alkaline Phosphatase Amylase Bilirubin Biopsy Blood Platelets Bone Marrow Calcium Corticotropin C Reactive Protein Creatinine Cytomegalovirus Electrocardiography Epstein-Barr Virus fibrin fragment D Hematologic Tests Hemoglobin Hepatitis B Hepatitis C virus Human T-lymphotropic virus 1 Hydrocortisone Lactate Dehydrogenase Leukocyte Count Luteinizing hormone Palpation Patients Physicians Potassium Sodium Spleen Thyrotropin Thyroxine Toxoplasmosis Uric Acid Vaccines Volumes, Packed Erythrocyte X-Ray Computed Tomography
4
Conditional Replicating Adenoviruses with Fc Fusions

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Publication 2023
Adenoviruses Adenovirus Vaccine A Fibers Anabolism Cytomegalovirus Deletion Mutation Genes Genome Peptides Service, Genetic Transgenes
5
Diagnosis of Acute Exacerbation in Idiopathic Pulmonary Fibrosis
AE in IPF was diagnosed according to the Japanese Respiratory Society diagnostic criteria as follows: (I) within one month of the chronic course of IPF disease progression, the following three conditions should be satisfied: (i) progressively worsening dyspnoea, (ii) new ground glass opacities evident on high-resolution computed tomography (CT) scans superimposed on a background reticular or honeycomb pattern , and (iii) a reduction of resting partial pressure of oxygen in arterial blood (PaO2) by more than 10 Torr (mmHg) compared to previous measurements; and (II) exclusion of obvious causes of acutely impaired respiratory function, such as infection, pneumothorax, cancer, pulmonary embolism, and congestive cardiac failure (8 (link),14 (link)).
Apparent infections were carefully excluded by measuring antibodies for Mycoplasma pneumoniae and Chlamydia pneumonia, β-D glucan, cytomegalovirus antigen and bacterial cultures of blood and sputum. Congestive heart failure was excluded by echocardiography and pulmonary embolism was excluded by contrast CT and/or echo-Doppler examination.
Arai T., Hirose M., Kagawa T., Hatsuda K, & Inoue Y. (2023). Interleukin-11 in idiopathic pulmonary fibrosis: predictive value of prognosis and acute exacerbation. Journal of Thoracic Disease, 15(2), 300-310.
Publication 2023
Antibodies Antigens Arteries Bacteria Blood Culture Congestive Heart Failure Cytomegalovirus Diagnosis Disease Progression Dyspnea Echocardiography ECHO protocol Glucans Hypoxia Infection Japanese Malignant Neoplasms Mycoplasma pneumoniae Partial Pressure Pneumonia, Chlamydial Pneumothorax Pulmonary Embolism Radionuclide Imaging Respiration Respiratory Rate Sputum X-Ray Computed Tomography

Top products related to «Cytomegalovirus»

1
Lipofectamine 2000 by Thermo Fisher Scientific
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
2
Dual luciferase reporter assay system by Promega
Sourced in United States, China, Germany, United Kingdom, Switzerland, Japan, France, Italy, Spain, Austria, Australia, Hong Kong, Finland
The Dual-Luciferase Reporter Assay System is a laboratory tool designed to measure and compare the activity of two different luciferase reporter genes simultaneously. The system provides a quantitative method for analyzing gene expression and regulation in transfected or transduced cells.
3
Pcdna3 by Thermo Fisher Scientific
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The PcDNA3.1 is a plasmid vector used for the expression of recombinant proteins in mammalian cells. It contains a powerful human cytomegalovirus (CMV) promoter, which drives high-level expression of the inserted gene. The vector also includes a neomycin resistance gene for selection of stable transfectants.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
6
Lipofectamine 3000 by Thermo Fisher Scientific
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Lipofectamine 3000 is a transfection reagent used for the efficient delivery of nucleic acids, such as plasmid DNA, siRNA, and mRNA, into a variety of mammalian cell types. It facilitates the entry of these molecules into the cells, enabling their expression or silencing.
7
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
8
Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
9
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
10
Lipofectamine 2000 reagent by Thermo Fisher Scientific
Sourced in United States, China, Germany, Canada, United Kingdom, Japan, France, Italy, Australia, Switzerland, Spain, Netherlands, Singapore, Cameroon, Colombia, Denmark, Lithuania
Lipofectamine 2000 reagent is a cationic lipid-based transfection reagent used for the delivery of nucleic acids, such as DNA and RNA, into eukaryotic cells. It facilitates the uptake of these molecules by the cells, enabling efficient gene expression or gene silencing studies.

More about "Cytomegalovirus"

Cytomegalovirus (CMV) is a common virus that belongs to the herpesvirus family.
It is a leading cause of congenital infections and often causes lifelong latent infections in humans.
CMV can lead to severe disease in immunocompromised individuals, such as those with HIV/AIDS or organ transplant recipients.
Researchers can optimize their CMV studies using PubCompare.ai, an AI-powered platform that helps identify the best protocols from literature, preprints, and patents.
Its cutting-edge comparison tools allow users to find the most reproducible and effective approaches, boosting research efficiency and productivity.
CMV is closely related to other herpesviruses, including Epstein-Barr virus (EBV) and herpes simplex virus (HSV).
These viruses can cause a range of symptoms, from mild cold sores to more serious conditions like encephalitis.
CMV in particular is known for its ability to reactivate and cause reinfection, even in individuals who have previously been exposed.
When studying CMV, researchers may utilize various laboratory techniques and reagents, such as Lipofectamine 2000 for transfection, the Dual-Luciferase Reporter Assay System for gene expression analysis, and pcDNA3.1 for vector construction.
Cell culture media like DMEM and fetal bovine serum (FBS) are commonly used, and antibiotics like penicillin and streptomycin help maintain sterile conditions.
Expereince the power of AI-driven protocol comparison today with PubCompare.ai, and unlock new insights to advance your CMV research.