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Enterovirus

Enteroviruses are a genus of positive-sense, single-stranded RNA viruses that can cause a wide range of illnesses, including hand, foot, and mouth disease, myocarditis, and aseptic meningitis.
These viruses are transmitted through direct contact with infected individuals or contaminated surfaces.
Enteroviurs research is crucial for understanding disease pathogenesis, developing effective treatments, and improving public health outcomes.
Pubcompare.ai can help optimize your Enterovirus research protocols, ensuring reproducibility and accuracy by identifying the most reliable and effective methods from the literature, preprints, and patents using AI-driven comparisons.
Experience the power of PubCompare.ai today and advance your Enterovirus research with confidence.

Most cited protocols related to «Enterovirus»

Epidemiology of Hand, Foot, and Mouth Disease in China

Cited 26 times

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Publication 2014

Diagnosis Enterovirus Fatal Outcome Virus

Comprehensive Respiratory Pathogen Detection

Cited 17 times

HBoV DNA positive samples were tested for 16 other potential pathogens, including influenza A virus, influenza B virus, parainfluenza virus (1, 2, 3, 4), respiratory syncytial virus, adenovirus, enterovirus, human metapneumovirus, human coronavirus (229E, OC43, NL63, HKU1), Mycoplasma pneumoniae, and Chlamydia pneumoniae by Taqman real-time PCR, in accordance with the manufacturer's protocol (Guangzhou HuYanSuo Medical Technology Co., Ltd).

Liu W.K., Chen D.H., Liu Q., Liang H.X., Yang Z.F., Qin S, & Zhou R. (2011). Detection of human bocavirus from children and adults with acute respiratory tract illness in Guangzhou, southern China. BMC Infectious Diseases, 11, 345.

Publication 2011

Adenoviruses Chlamydophila pneumoniae Coronavirus 229E, Human Enterovirus Human Metapneumovirus Influenza A virus Influenza B virus Mycoplasma pneumoniae Para-Influenza Virus Type 1 Pathogenicity Real-Time Polymerase Chain Reaction Respiratory Syncytial Virus

Multiplex PCR-Microsphere Flow Cytometry Assay

Cited 14 times

RMA is a new high-throughput, multiplex PCR-microsphere flow cytometry assay system for comprehensive detection of common respiratory viruses including rhinoviruses (HRV), enteroviruses, respiratory syncytial viruses (RSV), parainfluenza viruses, influenza viruses, metapneumoviruses, adenoviruses and coronaviruses. Details of the RMA assay have been previously described [33] (link). The output signal is expressed as MFI (median fluorescence intensity), and samples with an average signal >6 standard deviations of average negative control signals (typically 400 to 500 MFI) are regarded as positive. The RMA is capable of distinguishing closely related HRV and enteroviruses [33] (link).

Lee W.M., Kiesner C., Pappas T., Lee I., Grindle K., Jartti T., Jakiela B., Lemanske RF J.r., Shult P.A, & Gern J.E. (2007). A Diverse Group of Previously Unrecognized Human Rhinoviruses Are Common Causes of Respiratory Illnesses in Infants. PLoS ONE, 2(10), e966.

Publication 2007

Adenoviruses Biological Assay Coronavirus Enterovirus Flow Cytometry Fluorescence Metapneumovirus Microspheres Multiplex Polymerase Chain Reaction Orthomyxoviridae Parainfluenza Respiratory Syncytial Virus Respiratory System Rhinovirus Virus

Respiratory Viral Infections Epidemiology

Cited 12 times

Infections due to influenza A (FluA), adenovirus (AdV), bocavirus, human rhinovirus (HRV), influenza B (FluB), parainfluenza (PIV), coronavirus (CoV), respiratory syncytial virus A (RSVA), respiratory syncytial virus B (RSVB), enterovirus (EV) and human metapneumovirus (HMPV) were confirmed using RT-PCR via nasal wash products. Data were collected on admission including demographic information, comorbidities, routine laboratory examinations, chest radiography or CT scanning, immunological and etiological detections. We used positive bacterial culture of blood and sputum samples as the criteria for bacterial growth. The use of antiviral therapy and steroids was recorded, including the drug, start date, duration and dosage. Patients were evaluated as deemed clinically appropriate at any time when pneumonia was suspected. CURB-65 score of each patient was calculated (Barlow et al., 2007 (link)). Length of stay and outcome state of each patient were recorded. Those improved patients with hospital stay <90 days were followed up by a phone call to determine survival status if they were not seen in the outpatient clinic. Finally, the outcome of mortality was defined as overall mortality within 90 days.

Guo L., Wei D., Zhang X., Wu Y., Li Q., Zhou M, & Qu J. (2019). Clinical Features Predicting Mortality Risk in Patients With Viral Pneumonia: The MuLBSTA Score. Frontiers in Microbiology, 10, 2752.

Publication 2019

Adenovirus Infections Antiviral Agents Bacteria Blood Culture Bocavirus Coronavirus Enterovirus Herpesvirus 1, Cercopithecine Homo sapiens Human Metapneumovirus Human respiratory syncytial virus Infection Influenza Inpatient Nose Parainfluenza Patients Pharmaceutical Preparations Physical Examination Pneumonia Radiography, Thoracic Respiratory Rate Reverse Transcriptase Polymerase Chain Reaction Rhinovirus Sputum Steroids Therapeutics Vision

Defining Reference CSF WBC Counts in Infants

Cited 12 times

Infants less than or equal to 56 days of age were eligible for inclusion if they had a lumbar puncture performed as part of their emergency department evaluation between January 1, 2005 and June 30, 2007. Children in this age range were selected as they routinely undergo lumbar puncture when presenting with fever at our institution.28 (link), 29 (link) Patients undergoing lumbar puncture in the emergency department were identified using two different data sources to ensure accurate identification of all eligible infants: 1.) Emergency department computerized order entry records identified all infants with cerebrospinal fluid testing (including CSF gram stain, culture, cell count, glucose or protein) performed during the study period, and 2.) Clinical Virology Laboratory records identified all infants in whom CSF herpes simplex virus or enterovirus PCR testing was performed. Medical records of infants identified by these two sources were reviewed to determine study eligibility.
Figure 1 outlines major exclusion criteria used to derive the reference group. Patients were excluded sequentially if the lumbar puncture was traumatic or a condition known or suspected to cause CSF pleocytosis was present. In a traumatic lumbar puncture, the presence of red blood cells in the CSF alters WBC counts, and adjustment formulas cannot reliably approximate the actual values.30 (link)–33 (link) Conditions known or suspected to cause CSF pleocytosis include stroke, hydrocephalus, seizure on presentation, ventricular shunt or previous intracranial infection, congenital infection, herpes simplex virus meningoencephalitis, and bacterial meningitis.34 (link)–36 (link) Patients with serious bacterial illness including bacteremia, urinary tract infection, osteomyelitis, septic arthritis, pneumonia and bacterial gastroenteritis were also excluded as studies have identified CSF pleocytosis with non-central nervous system infections.36 (link)–38 (link) Infants may have met more than one of the exclusion criteria.
The remaining infants were divided based on whether or not testing for enterovirus was performed in the CSF by polymerase chain reaction and, if performed, whether the test result was positive or negative. Details of our approach to enterovirus PCR testing have been published previously.39 (link) As viral meningitis can cause CSF pleocytosis, patients with a positive CSF enterovirus PCR were excluded from the reference group.40 (link), 41 (link) While previous studies have examined preterm infants separately from term infants, CSF WBC counts are influenced by postnatal rather than postgestational age.42 (link) Our primary analysis, therefore, combined preterm and term infants into a single group.

Kestenbaum L.A., Ebberson J., Zorc J.J., Hodinka R.L, & Shah S.S. (2010). Defining Cerebrospinal Fluid White Blood Cell Count Reference Values in Neonates and Young Infants. Pediatrics, 125(2), 257-264.

Publication 2010

Arthritis, Bacterial Bacteremia Bacteria Central Nervous System Infection Cerebrospinal Fluid Cerebrovascular Accident Child Clinical Laboratory Services Diet, Formula Eligibility Determination Enterovirus Erythrocytes Fever Gastroenteritis Glucose Gram's stain Heart Ventricle Hydrocephalus Infant Infection Meningitis, Bacterial Meningoencephalitis, Herpetic Osteomyelitis Patients Pleocytosis Pneumonia Preterm Infant Proteins Punctures, Lumbar Seizures Simplexvirus Urinary Tract Infection Viral Meningitis

Most recents protocols related to «Enterovirus»

QMRA for Untreated Well Water Risks

Use QMRA to quantify the risk of GI associated with consuming untreated private well water and compare to Aim 1. Using approaches similar to other QMRA models of risk from well water consumption,45–47 (link) a multi-pathogen QMRA model will be developed using the untreated well water quality data collected from the subcohort of 180 households (270 samples). The following pathogens will be included in the QMRA: adenovirus, enterovirus, norovirus, diarrheagenic E. coli, Campylobacter, Salmonella, Shigella, Cryptosporidium, Giardia. The model will be developed before completing the final analysis of Aim 1 to allow for true unblinded comparison of the results. Individual models will be developed for each pathogen detected using established pathogen-specific dose-response curves. The concentration of pathogens found in the well water in our study will be fit to probability distributions and are the key inputs into the mathematical models. The model endpoint will be the annual number of cases/child-year for each pathogen. The results of the different pathogen-specific models will be combined to produce a total number of cases/child-year of GI expected in the 908 children studied in the trial. The final estimates will be reported as means with 90% probability intervals (PI) around the mean generated using Monte Carlo simulation (10 000 iterations using @Risk (Palisade, USA)). The PIs from the QMRA will be compared with the 90% CIs of intervention effect from Aim 1 to assess whether these intervals intersect.

Lee D., Denno D., Tarr P., Wu J., Stokdyk J.P., Borchardt M, & Murphy H.M. (2023). Study design and methods of the Wells and Enteric disease Transmission (WET) Trial: a randomised controlled trial. BMJ Open, 13(3), e068560.

Publication 2023

Adenoviruses AURKB protein, human Campylobacter Child Cryptosporidium Enterovirus Escherichia coli Giardia Households Norovirus pathogenesis Salmonella Shigella Water Consumption

Stool and Water Sampling for Enteric Pathogens

Families of the subcohort submit stools from their enrolled child to accompany the water samples, both at baseline and immediately following report of illness. We also ask families to submit one additional stool sample following another report of illness. This sample is not accompanied with a water sample due to budget limitations.
Stool sampling kits are provided and contain instructions, sterile specimen container with storage medium (Zymo DNA/RNA Shield; Zymo Research, Irvine, California, USA), sterile specimen container for samples without storage medium, collection ‘hat’ for toilet-trained children, insulated envelope, prepaid shipping label, gloves, biohazard bags and ice packs. Samples are mailed overnight to researchers at Temple University in Philadelphia. Subsections of neat samples are stored at −80°C. Aliquots of samples in storage medium are shipped on ice to the USDA/USGS laboratory in Marshfield, Wisconsin, USA, and are stored at −80°C until analysis. Nucleic acid extraction, reverse transcription and qPCR analysis are completed as described for water samples,13 (link) and pathogens are reported as present/absent. Samples are tested for noroviruses GI and GII, human adenovirus (groups A–F), enterovirus, hepatitis A virus, rotavirus (A and C), SARS-CoV-2, diarrheagenic E. coli, Salmonella, Shigella, Campylobacter, Giardia, and Cryptosporidium and Shiga toxin-producing bacteria (stx1 and stx2); online supplemental text S10 lists assay information.

Publication 2023

Adenoviruses, Human Bacteria Biohazards Biological Assay Campylobacter Child Commodes Cryptosporidium Enterovirus Escherichia coli Feces Giardia Hepatitis A virus Norovirus Nucleic Acids Pathogenicity Reverse Transcription Rotavirus Salmonella SARS-CoV-2 Shiga Toxin Shigella Sterility, Reproductive STX2 protein, human

Waterborne Pathogen Detection in Well Water and Stool

The presence of waterborne pathogens (adenovirus, enterovirus, hepatitis A virus, norovirus, rotavirus, diarrheagenic E. coli, Campylobacter, Salmonella, Shigella, Cryptosporidium, Giardia) in untreated well water samples and stools from children in the subcohort will be used to carry out Aims 1a (water), 2 (stool) and 3 (water and stool).

Publication 2023

Adenoviruses Campylobacter Child Cryptosporidium Enterovirus Escherichia coli Feces Giardia Hepatitis A virus Norovirus Pathogenicity Rotavirus Salmonella Shigella

Viral Pathogen Surveillance: Sampling and Detection

After obtaining consent, research staff collected nose and/or throat swabs and combined them in a viral transport medium (BD) if both were collected. Specimens were stored at 2–8 °C, transported to the laboratory, and divided into multiple aliquots. Total nucleic acid extraction was performed using the Roche MagNA Pure LC automated extraction system. Using reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays, all specimens were tested for rhinovirus (RV)/enterovirus, RSV, adenovirus (AdV), influenza (Flu) A, B, and C, metapneumovirus (MPV), parainfluenza virus (PIV)-1–4, and human RNase P (as an indicator of specimen quality). Cycle threshold (Ct) values served as a surrogate for viral load. Specimens were considered positive if the Ct value was less than 45 cycles. All laboratory results and clinical data were entered into a REDCap database [14 (link)]. We did not include the results of clinical viral testing in our study.

Amarin J.Z., Potter M., Thota J., Rankin D.A., Probst V., Haddadin Z., Stewart L.S., Yanis A., Talj R., Rahman H., Markus T.M., Chappell J., Lindegren M.L., Schaffner W., Spieker A.J, & Halasa N.B. (2023). Clinical characteristics and outcomes of children with single or co-detected rhinovirus-associated acute respiratory infection in Middle Tennessee. BMC Infectious Diseases, 23, 136.

Publication 2023

Adenovirus Infections Biological Assay Endoribonucleases Enterovirus Homo sapiens Metapneumovirus Nose Nucleic Acids Para-Influenza Virus Type 1 Parainfluenza Virus 4, Human Pharynx Reverse Transcriptase Polymerase Chain Reaction Rhinovirus

Multiplex RT-PCR for Respiratory Pathogens

Each specimen was first screened for RSV by means of the Resp-4-Plex kit (Abbott Molecular Inc., Des Plaines, IL, USA) used with the fully automated Alinity m System (Abbott Molecular Inc., Des Plaines, IL, USA) and according to the manufacturer’s instructions. This kit is a multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and differentiation of RNA from SARS-CoV-2, RSV, influenza A and B viruses. According to the manufacturer, the limit of detection is 0.300 and 0.100 median tissue culture infectious dose (TCID₅₀)/ml for RSV-A and RSV-B, respectively [30 ].
To discern RSV subgroup and detect the presence of other respiratory pathogens, samples positive for RSV were further tested by means of the Allplex Respiratory Panel (RP) assays (Seegene Inc.; Seoul, Republic of Korea) according to the manufacturer’s instructions. Briefly, nucleic acids were first extracted using the STARMag Universal Cartridge Kit (Seegene Inc.; Seoul, Republic of Korea) on the automated Nimbus IVD (Seegene Inc.; Seoul, Republic of Korea) platform. For this purpose, 200 µl of each specimen was extracted and eluted with 100 µl of elution buffer and set up for RT-PCR. RT-PCR was then performed on a CFX96 instrument (Bio-Rad Laboratories, Inc; Hercules, CA, USA) with the Allplex RPs 1–4 kits. These four panels are able to detect the most common respiratory pathogens - both viruses [RP 1: RSV-A, RSV-B, influenza viruses A, A(H1N1), A(H1N1)pdm09, A(H3N2) and B; RP 2: adenovirus (AdV), enterovirus (EV), metapneumovirus (MPV), parainfluenza (PIV) viruses 1–4; RP 3: bocaviruses (BoV) 1–4, coronaviruses (CoV) 229E, NL63, OC43, rhinovirus (RV)] and bacteria [RP 4: Streptococcus pneumoniae (SP), Bordetella parapertussis (BPP), Bordetella pertussis (BP), Chlamydophila pneumoniae (CP), Haemophilus influenzae (HI), Legionella pneumophila (LP), Mycoplasma pneumoniae (MP)]. For each RT-PCR, 8 µl of the extracted nucleic acid in a final volume of 25 µl was used. The diagnostic accuracy of this assay in detecting RSV-A and RSV-B is 100% [31 (link)].
Samples showing cycle threshold (Ct) values < 40 in at least one assay were deemed positive. Ct values were used as a proxy measure of viral load: lower Ct values indicate higher viral load.

Panatto D., Domnich A., Lai P.L., Ogliastro M., Bruzzone B., Galli C., Stefanelli F., Pariani E., Orsi A, & Icardi G. (2023). Epidemiology and molecular characteristics of respiratory syncytial virus (RSV) among italian community-dwelling adults, 2021/22 season. BMC Infectious Diseases, 23, 134.

Publication 2023

Adenoviruses Bacteria Biological Assay Bocavirus Bordetella parapertussis Bordetella pertussis Buffers Chlamydophila pneumoniae Coronavirus 229E, Human Diagnosis Enterovirus Haemophilus influenzae Herpesvirus 1, Cercopithecine Infection Influenza Legionella pneumophila Metapneumovirus Mycoplasma pneumoniae Nucleic Acids Orthomyxoviridae Parainfluenza Pathogenicity Real-Time Polymerase Chain Reaction Respiratory Rate Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Rhinovirus SARS-CoV-2 Streptococcus pneumoniae Tissues Virus

Top products related to «Enterovirus»

Qiaamp viral rna mini kit by Qiagen

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The QIAamp Viral RNA Mini Kit is a laboratory equipment designed for the extraction and purification of viral RNA from various sample types. It utilizes a silica-based membrane technology to efficiently capture and isolate viral RNA, which can then be used for downstream applications such as RT-PCR analysis.

Anyplex 2 rv16 detection kit by Seegene

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The Anyplex II RV16 Detection Kit is a real-time PCR-based diagnostic assay designed to detect and identify 16 common respiratory viruses, including influenza A/B, respiratory syncytial virus A/B, and human rhinovirus, among others. The kit provides a rapid and sensitive method for the simultaneous detection of these respiratory pathogens.

Nuclisens easymag by bioMérieux

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The NucliSENS easyMAG is a laboratory instrument designed for the automated extraction and purification of nucleic acids, including DNA and RNA, from a variety of sample types. It utilizes magnetic bead-based technology to efficiently isolate and concentrate the target molecules for downstream analysis.

Onestep rt pcr kit by Qiagen

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The OneStep RT-PCR kit is a laboratory reagent designed for the reverse transcription and amplification of RNA samples in a single reaction. It enables the conversion of RNA to complementary DNA (cDNA) and subsequent PCR amplification of the cDNA target sequence.

Seeplex rv15 ace detection kit by Seegene

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The Seeplex® RV15 ACE Detection Kit is a molecular diagnostic test designed for the simultaneous detection and identification of 15 different respiratory viruses. The kit utilizes the Seegene's proprietary DPO (Dual Priming Oligonucleotide) and TOCE (Tagging Oligonucleotide Cleavage and Extension) technologies to provide accurate and reliable results.

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More about "Enterovirus"

Enteroviruses, a genus of positive-sense, single-stranded RNA viruses, are known to cause a wide range of illnesses, including hand, foot, and mouth disease, myocarditis, and aseptic meningitis.
These viruses are transmitted through direct contact with infected individuals or contaminated surfaces.
Enterovirus research is crucial for understanding disease pathogenesis, developing effective treatments, and improving public health outcomes.
To optimize your Enterovirus research protocols, PubCompare.ai can be a valuable tool.
This AI-driven platform helps identify the most reliable and effective methods from the literature, preprints, and patents.
By comparing various protocols, PubCompare.ai ensures the reproducibility and accuracy of your research, empowering you to advance your Enterovirus studies with confidence.
When conducting Enterovirus research, researchers may utilize a variety of kits and tools, such as the QIAamp Viral RNA Mini Kit for RNA extraction, the Anyplex II RV16 Detection Kit for virus detection, the NucliSENS easyMAG for automated nucleic acid extraction, and the OneStep RT-PCR kit for reverse transcription and PCR amplification.
The Seeplex® RV15 ACE Detection Kit can also be used for the simultaneous detection of multiple respiratory viruses, including Enteroviruses.
Additionally, essential materials like fetal bovine serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM) may be used in cell culture experiments.
The CFX96 Real-Time PCR Detection System can be employed for quantitative PCR analysis, while the RNeasy Mini Kit may be utilized for total RNA extraction from various sample types.
By leveraging the power of PubCompare.ai and employing these specialized kits and tools, researchers can optimize their Enterovirus research protocols, ensuring reproducibility, accuracy, and ultimately, advancing our understanding of these important pathogens.