Log In Sign up
The largest database of trusted experimental protocols
> Living Beings > Virus > Suid Herpesvirus 1

Suid Herpesvirus 1

Suid Herpesvirus 1, also known as Pseudorabies virus, is a member of the Alphaherpesvirinae subfamily and the Varicellovirus genus.
It is a significant pathogen that primarily infects swine, causing a disease called Aujeszky's disease or pseudorabies.
The virus can also infect a wide range of other mammalian species, including cattle, sheep, goats, dogs, and humans, leading to a fatal encephalitis.
Suid Herpesvirus 1 is an important model organism for studying alphaherpesvirus biology and pathogenesis.
Research on this virus has implications for understanding and controlling viral infections in both veterinary and human medicin.

Most cited protocols related to «Suid Herpesvirus 1»

1
Visualizing Cortical Circuits in Awake Mice
All procedures were approved by the Janelia Farm Research Campus Institutional Animal Care and Use Committee. We used adult (> P60) male PV-IRES-cre mice (B6;129P2-Pvalbtm1(cre)Arbr/J, The Jackson Laboratory). All surgeries were conducted under isoflurane anesthesia (1.5–2%). Additional drugs reduced potential inflammation (Ketofen, 5mg/kg, subcutaneously) and provided local (Marcaine, 0.5% solution injected under the scalp) and general analgesia (Buprenorphine, 0.1 mg/kg, intraperitoneal). A circular piece of scalp was removed and the underlying bone was cleaned and dried. The periostium was removed with a dental drill and the exposed skull was covered with a thin layer of cyano-acrylic primer (Crazy glue). A custom-machined titanium frame was cemented to the skull with dental acrylic (Lang Dental).
Afferents from the somatosensory cortex were labeled with virus expressing tdTomato 33 (rAAV-CAG-tdTomato, serotype 2/1; 20 nl at 300 and 550 um depths). The C2 barrel was targeted based on intrinsic signal imaging 28 (link). The virus was injected with a custom, piston-based, volumetric injection system (based on a Narishige, MO-10, manipulator) 46 (link). Glass pipettes (Drummond) were pulled and beveled to a sharp tip (30 um outer diameter). Pipettes were back-filled with mineral oil and front-loaded with viral suspension immediately prior to injection.
A craniotomy was made over vM1 (size, 3×2mm; center relative to Bregma: lateral, 0.8 mm; anterior, 1 mm, left hemisphere, Fig. 2a–d). These coordinates were previously determined using intracortical microstimulation 8 (link),16 (link),18 (link), mapping axonal projections from vS1 in vM1 8 (link),47 (link), and trans-cellular labeling with pseudorabies virus (data not shown). Neurons underlying the craniotomy were labeled by injecting virus expressing GCamP3 (rAAV-syn-GCaMP3, serotype 2/1, produced by the University of Pennsylvania Gene Therapy Program Vector Core). The brain was covered with agar (2%). 4–8 sites (10–15 nl/site; depth, 150–210 um; rate, 10 nl/minute) were injected per craniotomy.
The imaging window was constructed from two layers of standard microscope coverglass (Fisher; # 2, thickness, 170 – 210 um), joined with a UV curable optical glue (NOR-61, Norland): a larger piece was attached to the bone; a smaller insert fit snugly into the craniotomy (Fig. 2b, d). The bone surrounding the craniotomy was thinned to allow for a flush fit between insert and the underlying dura.
After virus injection, the glass window was lowered into the craniotomy. The space between the glass and the bone was sealed off with a thin layer of agar (2%), and the window was cemented in place using dental acrylic (Lang Dental). At the end of the surgery, all whiskers on the right side of the snout except row C were trimmed. The mice recovered for 3 days before starting water restriction. Imaging sessions started 14–21 days after the surgery.
Huber D., Gutnisky D.A., Peron S., O’Connor D.H., Wiegert J.S., Tian L., Oertner G., Looger L.L, & Svoboda K. (2012). Multiple dynamic representations in the motor cortex during sensorimotor learning. Nature, 484(7395), 473-478.
Publication 2012
Adult Agar Axon Bones Brain Buprenorphine Cells Craniotomy Cranium Dental Anesthesia Dental Health Services Drill Dura Mater Flushing Genetic Vectors Inflammation Institutional Animal Care and Use Committees Internal Ribosome Entry Sites Isoflurane Males Management, Pain Marcaine Microscopy Mus Neurons Oil, Mineral Oligonucleotide Primers Operative Surgical Procedures Pharmaceutical Preparations Reading Frames Scalp Somatosensory Cortex Suid Herpesvirus 1 tdTomato Titanium Vibrissae Virus
2
Experimental PRRSV Infection in Piglets
Healthy, 6-week-old, landrace piglets that were free of PRRSV, classic swine fever virus (CSFV), pseudorabies virus (PRV), porcine circovirus type 2 (PCV2) and M. hyopneumoniae infections were obtained from Beijing Center for SPF Swine Breeding & Management. All piglets were confirmed to be negative for PRRSV and PCV2 antibodies, as determined using commercial ELISA kits and using RT-PCR or PCR for viral nucleic acid detection in sera. The animals were raised in the animal facilities at China Agricultural University (CAU). For the first batch of animal trials, forty-five piglets were randomly divided into nine groups. The animals in each group (n = 5) were separately raised in different isolation rooms. Each piglet in each infection group was intranasally administered with 2 ml of each virus containing 2×105 TCID50 (RvJXwn, RvJH1a, RvJH1b, RvJHSP, RvHB-1/3.9, RvHJ1a, RvHJ1b or RvHJSP). Each piglet in the control group was mock-inoculated with the same dose of MARC-145 cell culture supernatant. For the second batch of animal trials, fifty-five piglets were randomly allotted to eleven groups (n = 5). Each piglet in each infection group was intranasally inoculated with 2 ml of each virus containing 2×105 TCID50 (RvJHn9, RvJHn10, RvJHn9n10, RvJHn9n10n11, RvHJn9, RvHJn10, RvHJn9n10 or RvHJn9n10n11). The piglets in the control group were mock inoculated with 2 ml of MARC-145 cell culture supernatant. All the survived piglets were euthanized and necropsied on day 21 post-inoculation (pi).
Li Y., Zhou L., Zhang J., Ge X., Zhou R., Zheng H., Geng G., Guo X, & Yang H. (2014). Nsp9 and Nsp10 Contribute to the Fatal Virulence of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Emerging in China. PLoS Pathogens, 10(7), e1004216.
Publication 2014
Animals Antibodies CCL7 protein, human Cell Culture Techniques Classical Swine Fever Virus Enzyme-Linked Immunosorbent Assay Infection isolation ML-145 Nucleic Acids Pigs Porcine Circovirus Porcine respiratory and reproductive syndrome virus Reverse Transcriptase Polymerase Chain Reaction Serum Suid Herpesvirus 1 Vaccination Virus
3
PRRSV Strain Isolation and Characterization
Thirty-nine PRRSV strains were used in this study (Table 1). This set of strains included genotype-I (European) isolates from 1991 to 2006 of which some were retrieved from a viral collection (n = 15) and others were freshly isolated from frozen (-80 C) serum (n = 9) or lung tissue (n = 15) that have yielded positive results for PRRSV by RT-PCR. Freshly isolated viruses were from Spain and Portugal and archive viruses were from different countries of Western Europe. No epidemiological relationship was known to exist between the different isolates. Isolation was done in porcine alveolar macrophages (PAM) obtained from two healthy pigs free from all major diseases including PRRSV, pseudorabies virus and classical swine fever virus. Additionally, all PAM batches were tested for porcine circovirus type 2 (PCV2), hepatitis E virus and torque-tenovirus (TTV) according to previously described PCR protocols [14 (link)-16 (link)]. Viral stocks were also tested for mycoplasma by PCR. Viral stocks were produced from passages n = 2, n = 3 or n = 4 in PAM and, for each strain, batches of virus were larger enough to assure that the same batch could be used in all the experiments performed with that isolate, avoiding thus the use of different viral batches of the same strain for different experiments or replicas. Viral titrations were performed by inoculation of serial dilutions of viral stocks in PAM and readings were done by means of the immunoperoxidase monolayer assay using monoclonal antibodies for ORF5 (clon 3AH9, Ingenasa, Madrid, Spain) and ORF7 (clon 1CH5, Ingenasa, Madrid, Spain) using a method reported before with minor modifications [17 (link)].
In order to examine the need for virus viability for the induction of cytokines, three of the isolates were re-tested in parallel before and after inactivation by heat (60°C, 60 min). Complete inactivation was verified by inoculation of the heat-treated viral suspensions in PAM, which were examined at 72 h post-inoculation for the cytopathic effect and presence of PRRSV by IPMA. Untreated viable virus was used to assess the adequateness of the PAM batches for titrations.
Gimeno M., Darwich L., Diaz I., de la Torre E., Pujols J., Martín M., Inumaru S., Cano E., Domingo M., Montoya M, & Mateu E. (2011). Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates. Veterinary Research, 42(1), 9.
Publication 2011
Biological Assay Classical Swine Fever Virus Cytokine Cytopathogenic Effect, Viral Europeans Freezing Genotype Hepatitis E virus Immunoperoxidase Techniques isolation Lung Macrophages, Alveolar Monoclonal Antibodies Mycoplasma Pigs Porcine Circovirus Porcine respiratory and reproductive syndrome virus Reverse Transcriptase Polymerase Chain Reaction Serum Strains Suid Herpesvirus 1 Sus scrofa Technique, Dilution Tissues Titrimetry Torque Vaccination Viral Vaccines Virus Virus Viability
4
Isolation and Maintenance of Primary Porcine Alveolar Macrophages
Primary PAMs were isolated from 4-wk-old specific pathogen-free (SPF) piglets as previously described [26 (link), 27 ]. Animal experiments were approved by the Animal Ethics Committee at the institute and were performed in accordance with animal use ethical guidelines and approved protocols. Primary PAMs, iPAM cell lines and plat E cells were all maintained in Dulbecco’s modified Eagle medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotics (penicillin and streptomycin) at 37°C with a humidified atmosphere of 5% CO2. The highly pathogenic PRRSV strain HuN4 that we used was described previously [28 (link)]. PRRSV-SC-D is a NADC30-like strain that was isolated in our lab (unpublished data). The firefly luciferase-tagged Pseudorabies virus (PRV) was described previously [20 (link)].
Wang T.Y., Liu Y.G., Li L., Wang G., Wang H.M., Zhang H.L., Zhao S.F., Gao J.C., An T.Q., Tian Z.J., Tang Y.D, & Cai X.H. (2018). Porcine alveolar macrophage CD163 abundance is a pivotal switch for porcine reproductive and respiratory syndrome virus infection. Oncotarget, 9(15), 12174-12185.
Publication 2018
Animal Ethics Committees Animals Antibiotics Atmosphere Cell Lines Cells Eagle isophosphamide mustard Luciferases, Firefly Pathogenicity Penicillins PLAT protein, human Porcine respiratory and reproductive syndrome virus Specific Pathogen Free Strains Streptomycin Suid Herpesvirus 1
5
Isolation and Characterization of Porcine Alveolar Macrophages for ASFV Studies
The pulmonary alveolar macrophages (PAMs) used in this study were prepared from 2- to 3-month-old piglets. Briefly, the intact lung was filled with sterilized phosphate-buffered saline (PBS), and we then collected the bronchoalveolar lavage fluid. Cells were collected via centrifugation at 560 × g for 10 min and resuspended using PBS. After removing the erythrocytes and rinsing with PBS, the cell pellets were resuspended, and the cells were grown in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco). Cells were cultured in an incubator at 37°C under 5% CO2. We performed nucleic acid testing according to China national standards to ensure that there was no contamination with viruses infecting swine, which include ASFV, classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine parvovirus (PPV), and porcine circovirus 1/2 (PCV1/2).
The ASFV SY18 strain (GenBank accession no. MH766894), a field ASFV isolate, was isolated from pig specimens after the first outbreak of ASF in China in August 2018 by our laboratory, which caused acute infection in pigs and resulted in up to 100% fatality in the farm. The virus was passaged in primary PAMs and stored at −80°C. The SY18ΔMGF/CD2v strain, an attenuated strain of ASFV bearing deletions of the MGF505-1R, MGF505-2R, MGF505-3R, MGF360-12L, MGF505-13L, MGF505-14L, and EP402R ORFs through homologous recombination, was also used in this study for comparison in vaccination and challenge trials. It has been proven to protect vaccinated pigs from virulent isolate SY18 infection when challenged by the oral administration of 103.0 TCID50 (18 ).
ASFV strain SY18 was titrated using 96-well plates and observed by an immunofluorescence assay, with staining with a fluorescein isothiocyanate (FITC)-labeled monoclonal antibody against the ASFV protein p30. Briefly, virus suspensions were 10-fold diluted and inoculated onto the cell monolayer. After incubation for 5 days at 37°C, the cultures were fixed using 80% cold acetone and incubated with the FITC-labeled p30 monoclonal antibody (prepared in our laboratory) for 1 h at 37°C, followed by observation under a fluorescence microscope. For the gene-deleted strains, the fluorescence emitted directly from eGFP was observed. The Reed-Muench method was used to calculate the virus titer (43 (link)).
Zhang Y., Ke J., Zhang J., Yang J., Yue H., Zhou X., Qi Y., Zhu R., Miao F., Li Q., Zhang F., Wang Y., Han X., Mi L., Yang J., Zhang S., Chen T, & Hu R. (2021). African Swine Fever Virus Bearing an I226R Gene Deletion Elicits Robust Immunity in Pigs to African Swine Fever. Journal of Virology, 95(23), e01199-21.
Publication 2021
Acetone Administration, Oral Bronchoalveolar Lavage Fluid Cells Centrifugation Classical Swine Fever Virus Common Cold Erythrocytes Fetal Bovine Serum Fluorescein Fluorescence Gene Deletion Genes Homologous Recombination Immunofluorescence Infection isothiocyanate Lung Macrophages, Alveolar Microscopy, Fluorescence Monoclonal Antibodies Open Reading Frames Parvovirus, Porcine Pellets, Drug Phosphates Porcine circovirus 1 Porcine respiratory and reproductive syndrome virus Proteins Saline Solution Strains Suid Herpesvirus 1 Sus scrofa Vaccination Virus

Most recents protocols related to «Suid Herpesvirus 1»

1
Evaluating RAA Assay Specificity
Reference was made to published studies; four common and important DNA viruses were used to evaluate the specificity of the RAA assay [10 (link),50 (link)]. The five viruses are goat pox virus, sheep pox virus, African swine fever virus, bovine papular stomatitis virus and pseudorabies virus. African swine fever virus is stored in Biosafety Laboratory Level 3, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Viral RNA of the four viruses was extracted as a template to evaluate the specificity of the RAA assay.
Cui H., Guan J., Lu H., Liu J., Tu F., Zhang C., Su K., Guo Z, & Zhao K. (2023). Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay. Life, 13(2), 494.
Publication 2023
African Swine Fever Virus Biological Assay Cattle Chinese DNA Viruses Goatpox virus RNA, Viral Sheeppox virus Stomatitis Suid Herpesvirus 1 Virus
2
Recombinant Plasmid Construction for PCV4 and Related Viruses
Since it is difficult to isolate wild-type PCV4, the sequence of the PCV4-Cap gene (GenBank: OP221238) was synthesized, and sticky-end restriction enzymes were ligated for insertion into the pMD-19T vector (Takara, Dalian, China), resulting in a recombinant plasmid: pMD-19T-PCV4. PCV2 (GenBank: MK426833) was obtained from our laboratory repository. The recombinant plasmid pMD-19-PCV3-Cap was stored in our laboratory repository and was based on a PCV3 strain (GenBank: KY075995.1). Pseudorabies virus (PRV, Bartha-K61 vaccine strain), porcine reproductive and respiratory syndrome virus (PRRSV, CH-1R vaccine strain), and classical swine fever virus (CSFV, tissue culture origin) were purchased from the Harbin Veterinary Research Institute, China. Porcine parvovirus (PPV, PPVS-1A vaccine strain) was purchased from Qilu Animal Health Products Co., Ltd., China, and Japanese encephalitis virus (JEV) was purchased from Wuhan Keqian Biology Co., Ltd., China.
Wang J., Zhu X., Yin D., Cai C., Liu H., Yang Y., Guo Z., Yin L., Shen X., Dai Y, & Pan X. (2023). Rapid and Easy-Read Porcine Circovirus Type 4 Detection with CRISPR–Cas13a-Based Lateral Flow Strip. Microorganisms, 11(2), 354.
Publication 2023
Animals Classical Swine Fever Virus Cloning Vectors DNA Restriction Enzymes Genes Parvovirus, Porcine Plasmids Porcine respiratory and reproductive syndrome virus Strains Suid Herpesvirus 1 Tissues Vaccines Virus, Japanese Encephalitis
3
Quantifying Porcine Herpesvirus Genomic Copies
To determine the PRV genome copies in the nasal swabs and in the TG, total DNA was isolated as described previously [1 (link)] using the QIAamp® DNA mini kit (#51306, Qiagen, Hilden, North Rhine-Westphalia, Germany). Before DNA isolation from TG, tissues were homogenized using 2.8 mm ceramic beads (#15-340-154, Thermo Fisher Scientific®, Waltham, MA, USA) in Pre-cellys 24 homogenizer (#13112, Bertin Instruments, Rockville, MD, USA). To determine the transcription and quantification of the targeted immediate early (infected cell protein 0 - ICP0) and late (major capsid protein - MCP and glycoprotein C - gC) PRV genes in the TG neurons, RNA was isolated. Briefly, after the homogenization of TG, RNA was isolated using an RNeasy mini kit (#74104, Qiagen). DNA contamination in RNA samples was removed by RNase-Free DNase Set (#79254, Qiagen). Finally, cDNA synthesis was performed using the Verso cDNA synthesis kit (AB-1453/A, Thermo Fisher Scientific®).
PRVtmv+ genomic copies were determined by TaqMan probe-based real-time qPCR in ABI PRISM™ 7900HT Sequence Detection System (Applied Biosystems, Waltham, MA, USA), using targeted genes ICP0-, MCP-, and gC-specific primers and probes (Suid herpesvirus 1 strain Becker, GenBank accession # JF797219.1; Table 2). Each time, the PCR reaction setup was run with six standards of known quantity (101 to 106 copies per reaction).
The PRV gene copies were calculated by normalizing the MCP-specific CT values against the standard curve generated based on the CT values obtained for the known housekeeping gene, GAPDH copies (two copies/cell), in the same cells. The mean copies of the PRV-MCP gene per one million cells were then plotted.
For the PRVtmv+ latency control, we used the TG samples collected and stored at −80 °C from our previous vaccination experiment [1 (link)]. To demonstrate that RNA samples treated with the DNAse were free from residual DNA, treated RNA samples without cDNA synthesis were also used as controls. PRV genome copies in TG were normalized to endogenous host-specific swine housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH; GenBank accession #AF017079.1). The assays were performed in duplicate. The qPCR genome copy results are expressed as PRV genome copies per million cells. The RT-qPCR gene transcript copy results are expressed as transcript copies/ng of RNA.
Pavulraj S., Stout R.W., Paulsen D.B, & Chowdhury S.I. (2023). Live Triple Gene-Deleted Pseudorabies Virus-Vectored Subunit PCV2b and CSFV Vaccine Undergoes an Abortive Replication Cycle in the TG Neurons following Latency Reactivation. Viruses, 15(2), 473.
Publication 2023
Anabolism Biological Assay Capsid Proteins Cells Deoxyribonuclease I DNA, Complementary DNA Contamination Endoribonucleases GAPDH protein, human Genes Genes, Housekeeping Genome Glyceraldehyde-3-Phosphate Dehydrogenases glycoprotein gC, herpes simplex virus type 1 isolation Neurons Nose Oligonucleotide Primers prisma Proteins Strains Suid Herpesvirus 1 Sus scrofa Tissues Transcription, Genetic Vaccination
4
PRRSV Outbreak Management in Pigs
The study was carried out on a farrow-to-finish commercial pig farm with a capacity of 400 sows’ production (commercial hybrids of Large White × Landrace), located in southern Greece, from April to May 2021. Based on the results of routine blood sampling (breeding stock, weaning, growing, and finishing stage) the farm was PRRSV-infected. The results revealed positive samples in growers and finishers (90–160 days of age) for PRRSV type 1 (European genotype) using Real-Time Polymerase Chain Reaction (RT PCR). The clinical picture of animals was characterized mainly by respiratory symptoms including coughing, pyrexia, poor performance, and increased mortality due to secondary infections.
All sows of the farm were vaccinated against Aujeszky’s disease virus, parvovirus, atrophic rhinitis, erysipelas (Erysipelothrix rhusiopathiae), PRRSV (Modified Lived Vaccine-MLV), Escherichia coli, and Clostridium perfringens infections. Weaners were vaccinated against Porcine Circovirus type 2 and Mycoplasma hyopneumoniae. The PRRSV MLV vaccination of sows was performed as a mass vaccination every 3 months. Control of endo/ectoparasites was currently maintained by sows’ treatment with a single ivermectin injection 14 days before farrowing.
Papakonstantinou G.I., Gougoulis D.A., Voulgarakis N., Maragkakis G., Galamatis D., Athanasiou L.V, & Papatsiros V.G. (2023). Effects of Injectable Administration of Dexamethasone Alone or in Combination with Vitamin E/Se in Newborn Low Birth Weight Piglets. Veterinary Sciences, 10(2), 135.
Publication 2023
Animals Clostridium perfringens Infections Endometriosis Erysipelas Erysipelothrix rhusiopathiae Escherichia coli Europeans Fever Genotype Hybrids Ivermectin Mass Vaccination Mycoplasma hyopneumoniae Parvoviridae Infections Porcine Circovirus Porcine respiratory and reproductive syndrome virus Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Rhinitis, Atrophic Secondary Infections Signs and Symptoms, Respiratory Suid Herpesvirus 1 Vaccination Vaccines, Attenuated
5
Comprehensive Viral Strain Collection for Research
The following vaccine strains were purchased from Huapai Bioengineering Group Co. Ltd. (Chengdu, China) and other companies: Ch-1R strain of C-PRRSV, TJM92 strain of HP-PRRSV, C strain of CSFV, O/Mya98/XJ/2010 strain of foot-and-mouth disease virus (FMDV), ZJ/C strain of porcine circovirus type 2 (PCV2), Bartha-K61 strain of porcine pseudorabies virus (PRV), SC1 strain of porcine parvovirus (PPV), and TJ strain of swine influenza virus (SIV). The positive clinical samples of NL-PRRSV, ASFV, and Senecavirus A (SVA) were obtained by our laboratory and confirmed by genomic sequencing. They were stored at −70 °C until used.
Long F., Chen Y., Shi K., Yin Y., Feng S, & Si H. (2023). Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus. Animals : an Open Access Journal from MDPI, 13(4), 594.
Publication 2023
Foot-and-Mouth Disease Virus Genome Orthomyxovirus Type A, Porcine Parvovirus, Porcine Pigs Porcine Circovirus Porcine respiratory and reproductive syndrome virus Senecavirus A Strains Suid Herpesvirus 1 Vaccines

Top products related to «Suid Herpesvirus 1»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
3
Rpmi 1640 medium by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Japan, France, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Israel, Sweden, Denmark, Macao, Brazil, Ireland, India, Austria, Netherlands, Holy See (Vatican City State), Poland, Norway, Cameroon, Hong Kong, Morocco, Singapore, Thailand, Argentina, Taiwan, Province of China, Palestine, State of, Finland, Colombia, United Arab Emirates
RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
4
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
5
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
6
Qiaamp dna mini kit by Qiagen
Sourced in Germany, United States, France, United Kingdom, Netherlands, Spain, Japan, China, Italy, Canada, Switzerland, Australia, Sweden, India, Belgium, Brazil, Denmark
The QIAamp DNA Mini Kit is a laboratory equipment product designed for the purification of genomic DNA from a variety of sample types. It utilizes a silica-membrane-based technology to efficiently capture and purify DNA, which can then be used for various downstream applications.
7
Pv830 pneumatic picopump by World Precision Instruments
Sourced in United States
The PV830 Pneumatic PicoPump is a laboratory equipment used for precise and controlled fluid dispensing. It generates precise pneumatic pressure to deliver small, accurately-controlled liquid volumes.
8
Marc 145 cells by American Type Culture Collection
Sourced in United States
MARC-145 cells are a continuous cell line originating from the African green monkey kidney. They are commonly used as a model system for research applications, particularly related to virus infection and replication.
9
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
10
E z n a viral dna kit by Omega Bio-Tek
Sourced in United States
The E.Z.N.A.® Viral DNA Kit is a nucleic acid extraction kit designed to isolate viral DNA from various sample types. It utilizes a silica-based membrane technology to efficiently capture and purify viral DNA, providing high-quality samples for downstream applications.

More about "Suid Herpesvirus 1"

Suid Herpesvirus 1, also known as Pseudorabies virus (PRV), is a member of the Alphaherpesvirinae subfamily and the Varicellovirus genus.
It is a significant pathogen that primarily infects swine, causing a disease called Aujeszky's disease or pseudorabies.
The virus can also infect a wide range of other mammalian species, including cattle, sheep, goats, dogs, and even humans, leading to a fatal encephalitis.
Suid Herpesvirus 1 is an important model organism for studying alphaherpesvirus biology and pathogenesis.
Researchers often use cell lines like MARC-145 cells and culture media like DMEM, RPMI 1640, and FBS to propagate and study this virus.
Antibiotics like Penicillin and Streptomycin are commonly used to prevent bacterial contamination.
DNA extraction kits like the QIAamp DNA Mini Kit and E.Z.N.A.® Viral DNA Kit are useful for isolating viral genetic material for further analysis.
The PV830 Pneumatic PicoPump is a tool that can be utilized to precisely deliver small volumes of reagents and samples during Suid Herpesvirus 1 research.
Understanding the biology and pathogenesis of this virus has implications for controlling viral infections in both veterinary and human medicine.
With the help of advanced tools and techniques, researchers can optimize their Suid Herpesvirus 1 studies and make important discoveries that could lead to improved disease prevention and treatment strategies.