Flies were raised on standard corn meal Drosophila medium at 25°C. The markers and chromosomes are described in reference 34 , except as noted. The isolation and cloning of sqh1 is described in reference 25 (link). In the null allele sqhAX3, most of the sqh transcription unit encoding the RMLC had been removed by a 5-kb deletion. Its isolation will be described in detail elsewhere. The chromosome carrying sqhAX3 is marked with y, which allows hemizygous or homozygous mutant sqhAX3 larvae to be identified by their yellow mouth hooks. The stocks of FRT-101 and ovoD1 FRT-101/Y; hs-flp38 used to generate germline clones (9 (link)) were obtained from Dr. N. Perrimon. Wild-type controls used either FRT-101 or y w67, obtained from the Bloomington stock center.
Corn Flour
Corn flour is a finely ground powder made from dried corn kernels.
It is a common ingredient used in various food preparations, such as baked goods, sauces, and thickeners.
Corn flour provides a source of carbohydrates, fiber, and some vitamins and minerals.
It can be used to add texture, flavor, and nutritional value to recipes.
Researchers may consult the literature to optimize protocols and products related to corn flour usage and applications.
It is a common ingredient used in various food preparations, such as baked goods, sauces, and thickeners.
Corn flour provides a source of carbohydrates, fiber, and some vitamins and minerals.
It can be used to add texture, flavor, and nutritional value to recipes.
Researchers may consult the literature to optimize protocols and products related to corn flour usage and applications.
Most cited protocols related to «Corn Flour»
Alleles
Chromosomes
Clone Cells
Corn Flour
Deletion Mutation
Diptera
Drosophila
Germ Line
Hemizygote
Homozygote
isolation
Larva
Oral Cavity
Transcription, Genetic
Adult
Agar
Animals, Transgenic
Biological Assay
Canes
Carbohydrates
Corn Flour
Deletion Mutation
Diptera
Drosophila melanogaster
Ethanol
Food
Gene Expression Regulation
Light
Molasses
Nipagin
propionic acid
R-38486
Reproduction
Saccharomyces cerevisiae
Soybean Flour
Tetracycline
Wolbachia
In all experiments except those for RU486 calibration, we used our laboratory stock of outbred wild type Drosophila melanogaster, Dahomey, which has been cured of Wolbachia by tetracycline treatment 1 . Flies were maintained in large population cages with overlapping generations at 25 °C with a 12 h: 12 h light: dark cycle. UAS-ArcAβ42 and elavGS transgenic flies were backcrossed into w1118, as reported in 2 and wDah; dilp2-31,5 deletion mutants and Wolbachia-positive white Dahomey (wDah) controls are those reported in 3 . All genetic constructs were back-crossed into the genetic background of their control for at least six generations before experiments were performed.
For all experiments using adult flies, other than food choice, flies were reared on sugar, yeast food (1SYBrewer’s; 1SY) as described in 4 for lifespan experiments. Egg collections were used to synchronize fly age as described in 5 . Flies for the food choice assay were reared in a medium containing, per liter, 80 g cane molasses, 22 g beetroot syrup, 8 g agar, 80 g corn flour, 10 g soya flour, 18 g yeast extract, 8 ml propionic acid, 12 ml nipagin (15% in ethanol).
For all experiments using adult flies, other than food choice, flies were reared on sugar, yeast food (1SYBrewer’s; 1SY) as described in 4 for lifespan experiments. Egg collections were used to synchronize fly age as described in 5 . Flies for the food choice assay were reared in a medium containing, per liter, 80 g cane molasses, 22 g beetroot syrup, 8 g agar, 80 g corn flour, 10 g soya flour, 18 g yeast extract, 8 ml propionic acid, 12 ml nipagin (15% in ethanol).
Adult
Agar
Animals, Transgenic
Biological Assay
Canes
Carbohydrates
Corn Flour
Deletion Mutation
Diptera
Drosophila melanogaster
Ethanol
Food
Gene Expression Regulation
Light
Molasses
Nipagin
propionic acid
R-38486
Reproduction
Saccharomyces cerevisiae
Soybean Flour
Tetracycline
Wolbachia
Single ascospore or conidium isolates were prepared and grown on 2 % malt extract agar (MEA), or on 2 % corn meal agar plus 2 % w/v dextrose (CMD).
Growth of liquid culture and extraction of genomic DNA was performed as reported previously (Voglmayr & Jaklitsch 2011 , Jaklitsch et al. 2012 (link)) using the DNeasy Plant Mini Kit (QIAgen GmbH, Hilden, Germany) or the modified CTAB method of Riethmüller et al. (2002) (link).
The following loci were amplified and sequenced: the complete internal transcribed spacer region (ITS1-5.8S-ITS2) and a c. 900 bp fragment of the large subunit nuclear ribosomal DNA (nuLSU rDNA), amplified and sequenced as a single fragment with primers V9G (De Hoog & Gerrits van den Ende 1998 (link)) and LR5 (Vilgalys & Hester 1990 (link)); a 450–454 bp fragment of the calmodulin (cal) gene with primers CAL-228F and CAL-737R (Carbone & Kohn 1999 ); a 441–445 bp fragment of the histone H3 (his) gene with primers CYLH3F (Crous et al. 2004 ) and H3-1b (Glass & Donaldson 1995 (link)); a c. 1 kb fragment of the guanine nucleotide-binding protein subunit beta (ms204) gene with primers MS-E1F1 and MS-E5R1 (Walker et al. 2012 (link)); a 711 bp fragment of the RNA polymerase II subunit 1 (rpb1) gene with primers RPB1-Af and RPB1-Cr (Stiller & Hall 1997 (link)); a c. 1.2 kb fragment of the RNA polymerase II subunit 2 (rpb2) gene with primers fRPB2-5f and fRPB2-7cr (Liu et al. 1999 (link)) or dRPB2-5f and dRPB2-7cr (Voglmayr et al. 2016 (link)); a c. 1.3 kb fragment of the translation elongation factor 1-alpha (tef1) gene containing introns 4 and 5 and part of the exon with primers EF1-728F (Carbone & Kohn 1999 ) and TEF1LLErev (Jaklitsch et al. 2005 (link)); and a 441–445 bp fragment of the β-tubulin (tub2) gene with primers T1 (O’Donnell & Cigelnik 1997 (link)) and the newly designed BtHV2r (5’ CATCATRCGRTCNGGGAACTC 3’). PCR products were purified using an enzymatic PCR cleanup (Werle et al. 1994 (link)) as described in Voglmayr & Jaklitsch (2008) (link). DNA was cycle-sequenced using the ABI PRISM Big Dye Terminator Cycle Sequencing Ready Reaction Kit v. 3.1 (Applied Biosystems, Warrington, UK) and the PCR primers; in addition, primers ITS4 (White et al. 1990 ) and LR3 (Vilgalys & Hester 1990 (link)) were used as internal sequencing primers for the ITS-LSU rDNA region. Sequencing was performed on an automated DNA sequencer (ABI 3730xl Genetic Analyzer, Applied Biosystems).
Growth of liquid culture and extraction of genomic DNA was performed as reported previously (Voglmayr & Jaklitsch 2011 , Jaklitsch et al. 2012 (link)) using the DNeasy Plant Mini Kit (QIAgen GmbH, Hilden, Germany) or the modified CTAB method of Riethmüller et al. (2002) (link).
The following loci were amplified and sequenced: the complete internal transcribed spacer region (ITS1-5.8S-ITS2) and a c. 900 bp fragment of the large subunit nuclear ribosomal DNA (nuLSU rDNA), amplified and sequenced as a single fragment with primers V9G (De Hoog & Gerrits van den Ende 1998 (link)) and LR5 (Vilgalys & Hester 1990 (link)); a 450–454 bp fragment of the calmodulin (cal) gene with primers CAL-228F and CAL-737R (Carbone & Kohn 1999 ); a 441–445 bp fragment of the histone H3 (his) gene with primers CYLH3F (Crous et al. 2004 ) and H3-1b (Glass & Donaldson 1995 (link)); a c. 1 kb fragment of the guanine nucleotide-binding protein subunit beta (ms204) gene with primers MS-E1F1 and MS-E5R1 (Walker et al. 2012 (link)); a 711 bp fragment of the RNA polymerase II subunit 1 (rpb1) gene with primers RPB1-Af and RPB1-Cr (Stiller & Hall 1997 (link)); a c. 1.2 kb fragment of the RNA polymerase II subunit 2 (rpb2) gene with primers fRPB2-5f and fRPB2-7cr (Liu et al. 1999 (link)) or dRPB2-5f and dRPB2-7cr (Voglmayr et al. 2016 (link)); a c. 1.3 kb fragment of the translation elongation factor 1-alpha (tef1) gene containing introns 4 and 5 and part of the exon with primers EF1-728F (Carbone & Kohn 1999 ) and TEF1LLErev (Jaklitsch et al. 2005 (link)); and a 441–445 bp fragment of the β-tubulin (tub2) gene with primers T1 (O’Donnell & Cigelnik 1997 (link)) and the newly designed BtHV2r (5’ CATCATRCGRTCNGGGAACTC 3’). PCR products were purified using an enzymatic PCR cleanup (Werle et al. 1994 (link)) as described in Voglmayr & Jaklitsch (2008) (link). DNA was cycle-sequenced using the ABI PRISM Big Dye Terminator Cycle Sequencing Ready Reaction Kit v. 3.1 (Applied Biosystems, Warrington, UK) and the PCR primers; in addition, primers ITS4 (White et al. 1990 ) and LR3 (Vilgalys & Hester 1990 (link)) were used as internal sequencing primers for the ITS-LSU rDNA region. Sequencing was performed on an automated DNA sequencer (ABI 3730xl Genetic Analyzer, Applied Biosystems).
Agar
Calmodulin
Cetrimonium Bromide
Conidia
Corn Flour
DNA, Ribosomal
EEF1A1 protein, human
Enzymes
Exons
Genes
Genes, vif
Genome
Glucose
GNB1 protein, human
Histone H3
Introns
Oligonucleotide Primers
Plants
prisma
Protein Subunits
Reproduction
Ribosome Subunits, Large
RNA Polymerase II
Tubulin
Walkers
Animals, Transgenic
Corn Flour
Diptera
Drosophila
Embryo
Females
Genotype
Germ Line
Larva
Males
Microscopy
Molasses
tdTomato
Most recents protocols related to «Corn Flour»
Example 9
Gluten-free composite plant-MCT flour is made by replacing the gluten flour in Examples 1-7 with one or more gluten-free flours selected from oat flour, corn flour, white rice flour, buckwheat flour, sorghum flour, amaranth flour, teff flour, arrowroot flour, brown rice flour, chickpea flour, tapioca flour, cassava flour, tigernut flour, soy flour, potato flour, millet flour, or quinoa flour.
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Amaranth Dye
Buckwheat
Chickpea
Corn Flour
Eragrostis
Flour
Food
Gluten
Gluten-Free Diet
Manihot
Manihot esculenta
Maranta
Millets
Plants
Potato Flour
Quinoa
Rice Flour
Sorghum
Example 8
Reduced gluten composite plant-MCT flour is made by replacing 5-50% of the gluten flour in Examples 1-7 with one or more gluten-free flours selected from oat flour, corn flour, white rice flour, buckwheat flour, sorghum flour, amaranth flour, teff flour, arrowroot flour, brown rice flour, chickpea flour, tapioca flour, cassava flour, tigernut flour, soy flour, potato flour, millet flour, or quinoa flour.
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Amaranth Dye
Buckwheat
Chickpea
Corn Flour
Eragrostis
Flour
Food
Gluten
Gluten-Free Diet
Manihot
Manihot esculenta
Maranta
Millets
Plants
Potato Flour
Quinoa
Rice Flour
Sorghum
Hy-Line Brown laying hens were fed with a regular diet (corn-soybean meal-based; containing 0.32% non-phytate phosphorus (NPP); Table 1 ) start from 35 weeks of age. On the last day of age 40 weeks, a total of 60 hens that laid eggs between 07:30−08:30 were randomly selected to evaluate the daily phosphorus rhythms. Of them, 45 hens were euthanized for sample collection, and the other 15 hens were used to study the feed intake and calcium/phosphorus excretion rhythms. For sample collection, the 45 hens were sampled according the oviposition cycle: at oviposition, at 6, 12, 18 h post-oviposition, and at the next oviposition, respectively, with 9 hens sampled at each of the time point. The following samples were collected: blood (for serum), uterine (stored at −80 ℃, for Western-blotting analysis), femur (in 4% paraformaldehyde, for histological analysis) and kidney (stored at −80 ℃, for Western-blotting analysis). For the other 15 hens, the feed intake was recoded and the excreta was collected at the following intervals: from oviposition to 6 h post-oviposition, from 7 to 12 h post-oviposition, from 13 to 18 h post-oviposition, from 19 h post-oviposition to the next oviposition.
Composition and nutrient concentrations of basal diet (%, unless noted, as-is basis)
| Item | Low phosphorus | Regular phosphorus |
|---|---|---|
| Ingredients | ||
| Corn | 56.69 | 56.69 |
| Soybean meal | 25.77 | 25.77 |
| Distillers dried grains with solubles | 4.00 | 4.00 |
| Calcium carbonate | 9.73 | 9.04 |
| Dicalcium phosphate | - | 1.15 |
| Soybean oil | 1.51 | 1.51 |
| Sodium chloride | 0.26 | 0.26 |
| DL-Methionine | 0.18 | 0.18 |
| Choline chloride | 0.15 | 0.15 |
| Montmorillonite | 0.71 | 0.25 |
| Premix1 | 1 | 1 |
| In total | 100.00 | 100.00 |
| Nutrient levels | ||
| Metabolizable energy, kcal/kg (calculated) | 2,600 | 2,600 |
| Crude protein (calculated) | 16.5 | 16.5 |
| Total phosphorus (calculated/analyzed) | 0.34/0.34 | 0.53/0.49 |
| Non-phytate phosphorus (calculated) | 0.14 | 0.32 |
| Calcium (calculated/analyzed) | 3.50/3.47 | 3.50/3.52 |
1Provided per kilogram of diet: manganese 60 mg, copper 8 mg, zinc 80 mg, iodine 0.35 mg, selenium 0.3 mg, vitamin A 8000 IU, vitamin E 30 mg, vitamin K3 1.5 mg, thiamine 4 mg, riboflavin 13 mg, pantothenic acid 15 mg, nicotinamide 20 mg, pyridoxine 6 mg, biotin 0.15 mg, folic acid 1.5 mg, and cobalamin 0.02 mg
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Biotin
BLOOD
Calcium, Dietary
calcium phosphate
Cereals
Choline
Copper
Corn Flour
Corns
Diet
Eggs
Feed Intake
Femur
Folic Acid
Iodine
Kidney
Manganese
Niacinamide
Nutrients
Oviposition
Pantothenic Acid
paraform
Phosphorus
Phytate
Proteins
Pyridoxine
Riboflavin
Selenium
Serum
Sodium
sodium phosphate
Soybean Flour
Soybeans
Specimen Collection
Thiamine
Uterus
Vitamin A
Vitamin B12
Vitamin E
Vitamin K3
Western Blot
Zinc-80
SLBZS is composed of Panax Ginseng, Wolfiporia cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandiflorus and Glycyrrhiza uralensis Fisch, all herbs were purchased from Beijing Tongrentang Guangzhou pharmaceutical chain Co., Ltd (Guangzhou, China). Panax Ginseng, Wolfiporia cocos, Atractylodes macrocephala, Dioscorea opposita, Dolichos Lablab, Semen Nelumbinis, Semen Coicis, Fructus Amomi, Platycodon grandiflorus and Glycyrrhiza uralensis Fisch at a ratio of 4:4:4:4:3:2:2:2:2:4, pulverize in a beater and pass through a sieve of 60 mesh (19 (link), 20 (link), 22 (link)). 10g SLBZS powder was accurately weighed in a 250 ml conical flask, and 4% (corn flour by weight/substrate by weight) of corn flour was added to it. High-temperature sterilization was performed at 121°C for 20 min, and cooling to room temperature for later use.
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Atractylodes
Balloon Flower
Corn Flour
Dioscorea opposita
Dolichos
Fever
Fruit
Glycyrrhiza uralensis
Panax ginseng
Pharmaceutical Preparations
Plant Embryos
Powder
Sterilization
Wolfiporia extensa
All the experimental procedures were approved by the University of Illinois (Urbana-Champaign) Institutional Animal Care and Use Committee (no. 17166). In total, 16 multiparous Holstein cows past peak lactation (>60 DIM) were used in a randomized complete block design conducted from February to April 2020. All cows were housed in a freestall system equipped with individual feeding gates (American Calan Inc., Northwood, NH) and were fed once daily at approximately 0800 hours. Milking occurred two times per day at 0400 and 1530 hours. Cows were blocked by DIM (97.1 ± 7.6 d) and parity (3.4 ± 0.62), MY, BCS, and SCC (Supplemental Table 1 ). After 1 wk of adaptation, cows were randomly assigned within block to one of two treatments (eight per treatment): 1) a control group receiving the basal TMR with no supplementation (CON) or 2) basal TMR with 19 g/d of a Saccharomyces cerevisiae fermentation product (NTK; NutriTek®, Diamon V, Cedar Rapids, IA), top-dressed. This is the commercially recommended dose for the type of animal used in this trial. The ration was formulated to meet NRC (2001) requirements and is presented in Table 1 . The diet was formulated for cows at 180 DIM, BW of 703 kg, MY of 35.4 kg/d with a target of 3.50% milk fat and 3.12 milk protein and predicted DMI of 24.9 kg/d. The diet included corn silage, alfalfa hay, canola and corn gluten meal, ground shelled corn, rumen-protected lysine and methionine, rumen-inert fat, rumen-bypass protein, vitamin and mineral mix, and the ionophore Rumensin (Elanco, Greenfield, IN). The experimental period lasted 68 d and was divided into two phases: 1) Phase 1 (P1) from the beginning of the experimental period until d 63, and 2) Phase 2, from d 64 to 68, which represents the FR period. Cows were euthanized at the end of FR for a separate experiment. During Phase 2, cows were restricted to 40% of their ad libitum intake of the previous 5 d. This amount of restriction was chosen based off previous work in which 40% restriction was validated as a method to induce intestinal barrier dysfunction in dairy cattle (Kvidera et al., 2017b (link)).
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Acclimatization
Alfalfa
Animals
Calan
Cattle
Corn Flour
Corns
Fermentation
Gluten
Holstein Cow
Institutional Animal Care and Use Committees
Intestinal Diseases
Ionophores
Lactation
Lysine
Methionine
Milk, Cow's
Milk Proteins
Minerals
Proteins
Rumen
Rumensin
Saccharomyces cerevisiae
Silage
Therapy, Diet
Vitamins
Top products related to «Corn Flour»
Sourced in United States
W1118 is a wild-type Drosophila melanogaster strain commonly used as a genetic background. It serves as a standard reference strain for various experimental studies in the field of Drosophila research.
Sourced in United States, United Kingdom
The Elav-Gal4 is a genetic tool used in Drosophila research. It is a driver line that expresses the Gal4 transcription factor under the control of the elav (embryonic lethal abnormal vision) gene promoter, which is active in all post-mitotic neurons. This allows for the targeted expression of transgenes in the neuronal cells of Drosophila.
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Propionic acid is a widely used organic compound that serves as a key ingredient in various industrial and laboratory applications. It is a colorless, pungent liquid with a characteristic odor. Propionic acid is primarily utilized as a preservative and antimicrobial agent in food, animal feed, and pharmaceutical products.
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The BX51 microscope is an optical microscope designed for a variety of laboratory applications. It features a modular design and offers various illumination and observation methods to accommodate different sample types and research needs.
Sourced in Belgium, Germany, China, United States
Propionic acid is a colorless, liquid carboxylic acid with a pungent odor. It is commonly used in the chemical industry as a preservative, antimicrobial agent, and as an intermediate in the production of various organic compounds.
Sourced in Japan
The N-1300 is a laboratory equipment that serves as a nitrogen evaporator. It is designed to efficiently remove solvents from samples through evaporation, concentrating the desired components for further analysis or processing.
Sourced in Germany
Corn meal agar is a type of microbiological culture medium. It is used for the cultivation and isolation of various fungi, including yeasts and molds. The agar provides a nutrient-rich environment that supports the growth of these microorganisms.
Sourced in United States
Elav-GAL4 is a transgenic fly line that expresses the yeast transcriptional activator GAL4 under the control of the elav (embryonic lethal, abnormal vision) promoter. The elav gene is expressed in all postmitotic neurons in Drosophila, making Elav-GAL4 a useful tool for driving the expression of target genes in the nervous system of the fly.
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The Milli-Q water purification system is a laboratory equipment designed to produce high-quality, ultrapure water. The system utilizes a combination of technologies, including reverse osmosis and deionization, to remove impurities and contaminants from the input water, resulting in water that meets the stringent purity requirements for various laboratory applications.
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The PDA is a versatile laboratory equipment designed for various applications. It serves as a personal digital assistant, providing users with a compact and portable platform for data collection, analysis, and management. The core function of the PDA is to facilitate efficient data handling and processing within the laboratory environment.
More about "Corn Flour"
Corn flour, also known as maize flour or cornstarch, is a finely ground powder made from dried corn kernels.
It is a versatile ingredient used in a wide variety of food preparations, such as baked goods, sauces, gravies, and thickeners.
Corn flour provides a source of carbohydrates, fiber, and essential vitamins and minerals, making it a nutritious addition to recipes.
Researchers and food scientists may consult the literature to optimize protocols and products related to corn flour usage and applications.
This includes exploring the use of corn flour in traditional and modern culinary applications, as well as investigating its potential for enhancing texture, flavor, and nutritional value in various food items.
The PubCompare.ai platform, an AI-driven tool, can be particularly useful for streamlining the research process and identifying the best protocols and products related to corn flour from scientific literature, preprints, and patents.
This powerful AI-driven comparison tool can help researchers make more informed decisions and optimize their corn flour-related protocols and products.
In addition to its culinary applications, corn flour has also been used in the production of biofuels, as a source of industrial starch, and in the development of specialized corn-based products, such as corn meal agar and Milli-Q water purification systems.
The versatility of corn flour makes it an important ingredient in various industries, from food and agriculture to biotechnology and environmental sciences.
Whether you're a chef, food scientist, or researcher, understanding the properties, uses, and optimization techniques for corn flour can be invaluable in your work.
Explore the wealth of information available on this versatile ingredient and unlock the full potential of corn flour in your projects.
It is a versatile ingredient used in a wide variety of food preparations, such as baked goods, sauces, gravies, and thickeners.
Corn flour provides a source of carbohydrates, fiber, and essential vitamins and minerals, making it a nutritious addition to recipes.
Researchers and food scientists may consult the literature to optimize protocols and products related to corn flour usage and applications.
This includes exploring the use of corn flour in traditional and modern culinary applications, as well as investigating its potential for enhancing texture, flavor, and nutritional value in various food items.
The PubCompare.ai platform, an AI-driven tool, can be particularly useful for streamlining the research process and identifying the best protocols and products related to corn flour from scientific literature, preprints, and patents.
This powerful AI-driven comparison tool can help researchers make more informed decisions and optimize their corn flour-related protocols and products.
In addition to its culinary applications, corn flour has also been used in the production of biofuels, as a source of industrial starch, and in the development of specialized corn-based products, such as corn meal agar and Milli-Q water purification systems.
The versatility of corn flour makes it an important ingredient in various industries, from food and agriculture to biotechnology and environmental sciences.
Whether you're a chef, food scientist, or researcher, understanding the properties, uses, and optimization techniques for corn flour can be invaluable in your work.
Explore the wealth of information available on this versatile ingredient and unlock the full potential of corn flour in your projects.