Tamoxifen was prepared by first dissolving in ethanol (20 mg/500 μl) and mixing this solution with 980 μl corn oil for a final concentration of 20 mg/ml. Ethanol was then removed with a heated speed vacuum. Mice containing CreERT2 that were ~2 months old were injected with approximately 200 μl tamoxifen solution (200 mg/kg) once a day over 5 days. Animals were monitored for adverse effects, and if these became apparent, treatment was stopped. One week after treatment ended, animals were processed as described below for ISH or localization of XFP.
Corn oil
Corn oil is a vegetable oil derived from the germ of corn (Zea mays).
It is commonly used in cooking, baking, and as an ingredient in various food products.
Corn oil is rich in polyunsaturated fatty acids, such as linoleic acid, and has a relatively high smoke point, making it suitable for high-heat cooking.
This oil is also used in the production of biodiesel and as a lubricant.
Researchers can optimie their corn oil research using PubCompare.ai's AI-driven platform, which helps locate protocols from literature, preprints, and patents, while utilizing smart comparisons to identify the best protocols and products.
This can enhance the accuaracy of corn oil research by leveraging PubCompare.ai's powerful tools.
It is commonly used in cooking, baking, and as an ingredient in various food products.
Corn oil is rich in polyunsaturated fatty acids, such as linoleic acid, and has a relatively high smoke point, making it suitable for high-heat cooking.
This oil is also used in the production of biodiesel and as a lubricant.
Researchers can optimie their corn oil research using PubCompare.ai's AI-driven platform, which helps locate protocols from literature, preprints, and patents, while utilizing smart comparisons to identify the best protocols and products.
This can enhance the accuaracy of corn oil research by leveraging PubCompare.ai's powerful tools.
Most cited protocols related to «Corn oil»
Aftercare
Animals
Corn oil
Ethanol
Mice, House
Tamoxifen
Vacuum
Tamoxifen was prepared by first dissolving in ethanol (20 mg/500 μl) and mixing this solution with 980 μl corn oil for a final concentration of 20 mg/ml. Ethanol was then removed with a heated speed vacuum. Mice containing CreERT2 that were ~2 months old were injected with approximately 200 μl tamoxifen solution (200 mg/kg) once a day over 5 days. Animals were monitored for adverse effects, and if these became apparent, treatment was stopped. One week after treatment ended, animals were processed as described below for ISH or localization of XFP.
Aftercare
Animals
Corn oil
Ethanol
Mice, House
Tamoxifen
Vacuum
For chromatin immunoprecipitation (ChIP), 5 month-old male C57BL/6J mice were euthanized by CO2 asphyxiation at ZT 8. Livers were removed and pooled for ChIP, processing and sequencing. Tamoxifen-induction of Cre Recombinase activity was accomplished by daily intraperitoneal injection of 2 mg tamoxifen (Sigma) in 100μl corn oil (Sigma) for 7 days. 3 month-old tamoxifen-treated animals were subjected to wheel running assays for 7 days after the end of treatment. For gene expression analysis, 5–6 month-old males were euthanized by cervical dislocation at indicated ZT points for rapid dissection and snap-freezing of the tissues. During the dark cycle, procedures were performed under red light. Detailed materials and methods are provided in the Supplementary Information .
Animals
Asphyxia
Biological Assay
Corn oil
Cre recombinase
Dissection
Gene Expression Profiling
Immunoprecipitation, Chromatin
Injections, Intraperitoneal
Joint Dislocations
Light
Liver
Males
Mice, Inbred C57BL
Neck
Tamoxifen
Tissues
Animals
Asphyxia
Biological Assay
Corn oil
Cre recombinase
Dissection
Gene Expression Profiling
Immunoprecipitation, Chromatin
Injections, Intraperitoneal
Joint Dislocations
Light
Liver
Males
Mice, Inbred C57BL
Neck
Tamoxifen
Tissues
All mice used in these studies were maintained according to protocols approved by the Institutional Animal Care and Use Committee of the New York University School of Medicine. Double homozygous male mice (Nkx6-2CreER/CreER; RCE:loxP) were intercrossed with Nkx6-2LacZ heterozygote females (Nkx6-2LacZ/+) (Vallstedt et al. 2001 (link)) to generate experimental control (Nkx6-2CreER/+; RCE:loxP) and mutant (Nkx6-2CreER/LacZ; RCE:loxP) mice. Pregnant females were administered 4 mg tamoxifen (Sigma, St Louis, MO) (20 mg/mL dissolved in corn oil [Sigma]) at 12:00–2:00 PM on either E10.5 or E12.5 by gavaging with silicon-protected needles. When pregnant mothers did not deliver pups by noon of E19.5, a cesarean section was performed and pups were fostered. PCR genotyping of the Nkx6-2WT, Nkx6-2LacZ, and RCE:loxP alleles was performed using the following sets of primers: N6.2wt-S (5′-GAGATGAAGACGTCGCTGTTCC-3′), N6.2wt-AS (5′-CTGCCAAATACTTGGTCTGCTCG-3′), N6.2wt-S2 (5′-GAGATGAAGACGTCGCTGTTCC-3′), bTAU (5′-CCTGGATAACATCACACACGTCC-3′), LacZ129 (5′-ACGACTGTCCTGGCCGTAACCGACCC-3′), RCE-Rosa1 (5′-CCCAAAGTCGCTCTGAGTTGTTATC-3′), RCE-Rosa2 (5′-GAAGGAGCGGGAGAAATGGATATG-3′), and RCE-Cag3 (5′-CCAGGCGGGCCATTTACCGTAAG-3′). The Nkx6-2CreER allele was genotyped by using primers specific to the CRE recombinase.
Alleles
Cesarean Section
Corn oil
Cre recombinase
Females
Heterozygote
Homozygote
Institutional Animal Care and Use Committees
LacZ Genes
Males
Mice, House
Mothers
Needles
Oligonucleotide Primers
Pharmaceutical Preparations
Pregnant Women
Silicon
Tamoxifen
Most recents protocols related to «Corn oil»
Example 12
The composite plant-MCT flour of any of Examples 1-11 is modified by including one or more supplemental oils in addition to and/or that replace a portion of the MCT oil, including one or more of avocado oil, Brazil nut oil, canola oil, corn oil, cottonseed oil, flaxseed oil, grape see oil, hemp seed oil, olive oil, palm oil, peanut oil, rice bran oil, safflower oil (e.g., high oleic), sesame oil, soybean oil, walnut oil, hazelnut oil, sunflower oil, or butterfat.
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Brazil Nuts
Canola Oil
Corn oil
Flour
Food
Grapes
Hazelnuts
hempseed oil
Juglans
Linseed oil
MCTS1 protein, human
Oil, Cottonseed
Oil, Olive
Oil, Sunflower
Palm Oil
Peanut Oil
Persea americana
Plants
Rice Bran Oil
Safflower oil
Sesame Oil
SLC16A11 protein, human
Soybean oil
The animals were randomly divided into control and treated groups 12 mice in each (six males and six females). The control groups were further divided into sham (no treatment) and vehicle (sterile corn oil), whereas the treatment group was divided into VitD (2,195 IU/kg), BPA (50 μg/kg), and BPA+VitD (50 μg/kg + 2,195 IU/kg) groups. The BPA and VitD doses were administered based on previously recommended doses [23 (link)–27 (link)]. Corn oil, VitD, and BPA were administrated daily, intraperitoneally (i.p), for six weeks. One week later, at 10.5 weeks old, animals were sacrificed, and blood and spleen samples were collected for biochemical and histological examinations (Fig 1 ).
The mice were administered intraperitoneally with 50 μg VitD, 2195 IU BPA/kg body weight, either alone or in combination, respectively, for six weeks.
The mice were administered intraperitoneally with 50 μg VitD, 2195 IU BPA/kg body weight, either alone or in combination, respectively, for six weeks.
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Animals
BLOOD
Body Weight
Corn oil
Females
Males
Mice, House
Physical Examination
Spleen
Sterility, Reproductive
Vitamin D
The animal experiments were conducted under a protocol (2019-X15-03) that the ethics committee approved by the Laboratory Animals Center of Chinese PLA General Hospital (Beijing). Twenty-one male C57BL/6J mice (weight 30 g, aged 8–10 weeks) were purchased from Sipeifu (Beijing, China) and randomly divided into three groups to obtain liver tissue for molecular and histological studies (n = 7/group): mice with corn oil only without CCl4 injury (NC), mice with corn oil and CCl4 injury (CCl4), and mice with Apcin and CCl4 injury (Apcin). Before the CCl4 injection, the Apcin mice group was intraperitoneally injected with Apcin (30 mg/kg) dissolved in corn oil. Eight hours later, the CCl4 and Apcin mice were given CCl4 2.9 mL/kg dissolved in corn oil (Solarbio, C7030) by intraperitoneal injection. The mice in the NC group were injected with corn oil as a control. After 24 h of CCl4 treatment, all mice were sacrificed to obtain serum and liver tissues. Another 21 mice were randomly divided into three groups (n = 7/groups) to obtain the survival rate of ALF, as described above. Seven days after injection, all mice were sacrificed, and the survival rate can be further analyzed.
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Aftercare
Animals, Laboratory
apcin
CCL4 protein, human
Chinese
Corn oil
Ethics Committees
Injections, Intraperitoneal
Injuries
Liver
Males
Mice, House
Mice, Inbred C57BL
Serum
Tissues
Adoptive transfers were performed by intravenous injection of 5 × 103 Igλ-enriched B1-8 B cells into the retro-orbital plexus of anesthetized mice. Mice were immunized the following day. All immunizations were performed using NP-CGG (Biosearch Technologies) resuspended at 1 mg/ml in D-PBS and mixed 50:50 volumetrically with Alhydrogel (Accurate Chemical and Scientific). Mice were injected subcutaneously with 20 μl of this solution (10 μg of NP-CGG per injection) in each ear. At endpoint, the facial LNs from each side were pooled for analysis (see figure legends for various timepoints).
Unmodified αIgE (clone R1E4; produced by hybridoma culture as described below), αIgE with a mutated Fc-receptor binding domain (clone R1E4; Cedarlane), or control rat γ globulin were diluted in D-PBS to a concentration of 0.3 mg/ml and injected intravenously to achieve a final dose of 3.25 mg/kg. For the experiment shown, mouse γ globulin (Jackson ImmunoResearch) was used as a control whereas in previous experiments that the presented data are representative of rat γ globulin was used as a control.
Tamoxifen was dissolved at 50 mg/ml in corn oil (Sigma-Aldrich) by shaking at 56°C for several hours. Approximately 100 μl/mouse was delivered by intraperitoneal injection to achieve a dose of 200 mg/kg.
Unmodified αIgE (clone R1E4; produced by hybridoma culture as described below), αIgE with a mutated Fc-receptor binding domain (clone R1E4; Cedarlane), or control rat γ globulin were diluted in D-PBS to a concentration of 0.3 mg/ml and injected intravenously to achieve a final dose of 3.25 mg/kg. For the experiment shown, mouse γ globulin (Jackson ImmunoResearch) was used as a control whereas in previous experiments that the presented data are representative of rat γ globulin was used as a control.
Tamoxifen was dissolved at 50 mg/ml in corn oil (Sigma-Aldrich) by shaking at 56°C for several hours. Approximately 100 μl/mouse was delivered by intraperitoneal injection to achieve a dose of 200 mg/kg.
Adoptive Transfer
Alhydrogel
B-Lymphocytes
Clone Cells
Corn oil
Face
Fc Receptor
gamma-Globulin
Hybridomas
Immunization
Injections, Intraperitoneal
Mice, House
NP 10
Tamoxifen
Adult Vdrf/f and Cx3cr1CreER (#020940, Jackson Laboratory) mice of C57BL/6 background were mated to generate Vdrf/fCx3cr1CreER/+ mice. Vdrf/f mice were kindly provided by Professor James C. Fleet from Purdue University. Mice were housed under specific pathogen-free conditions with sufficient food and water. Vitamin D and progesterone have been shown to play synergistic roles in relieving acute brain injury following cerebral ischemia [26 (link), 27 (link)]. Hence, to control the confounding impact of endogenous estrogen and progesterone, male adult animals were primarily included as the studied subjects for in vivo experiments. Male Vdrf/fCx3cr1CreER/+ mice were used as Vdr conditional knockout (Vdr-cKO) group, and age matched male Vdrf/f mice were used as controls for in vivo experiments. The mice (8–10 weeks old) were given tamoxifen (75 mg/kg/d, Sigma) dissolved in corn oil intraperitoneally (i.p.) for five consecutive days 2 weeks before the middle cerebral artery occlusion (MCAO) procedure. Neonatal mice were used to obtain primary cells for in vitro experiments. Animal numbers for all experiments are indicated in the figure legends. All experimental protocols completely adhered to ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines and were approved by the Animal Care and Use Committee of Xuanwu Hospital (AEEI-2021-295). Adhering to the ARRIVE guidelines, we here designated neurobehavioral functions and infarct volumes as the primary endpoints.
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6-(N-(4-aminoethyl)-N-ethyl)amino-2,3-dihydro-1,4-phthalazine-1,4-dione
Adult
Animals
Cells
Cerebral Ischemia
Corn oil
Ergocalciferol
Estrogens
Food
Infant, Newborn
Infarction
Injuries, Acute Brain
Males
Mice, Inbred C57BL
Middle Cerebral Artery Occlusion
Mus
Progesterone
Specific Pathogen Free
Tamoxifen
Top products related to «Corn oil»
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Tamoxifen is a drug used in the treatment of certain types of cancer, primarily breast cancer. It is a selective estrogen receptor modulator (SERM) that can act as both an agonist and antagonist of the estrogen receptor. Tamoxifen is used to treat and prevent breast cancer in both men and women.
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Corn oil is a versatile laboratory product derived from the kernels of corn. It serves as a useful medium for various applications in the scientific and research fields. The oil's core function is to provide a consistent and reliable source of lipids for experimental purposes.
Sourced in United States, Germany
The C8267 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use. The core function of the C8267 is to assist in various experimental and analytical processes within a laboratory setting.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
Sourced in United States, Germany
Tamoxifen (TAM) is a selective estrogen receptor modulator (SERM) used as a laboratory reagent. It functions by binding to and modulating the activity of estrogen receptors in cells.
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4-hydroxytamoxifen is a laboratory reagent used in scientific research. It is a metabolite of the anti-cancer drug tamoxifen. The core function of 4-hydroxytamoxifen is to serve as a tool for researchers to investigate cellular processes and pathways.
Sourced in United States, United Kingdom, Germany, China
4-OHT is a laboratory reagent used in cell biology research. It is a selective estrogen receptor modulator (SERM) that can be used to induce gene expression in cell lines engineered with the CreERT2 system. The core function of 4-OHT is to enable temporal control of Cre recombinase activity in such cell lines.
Sourced in United States, Montenegro, Japan, Canada, United Kingdom, Germany, Macao, Switzerland, China
C57BL/6J mice are a widely used inbred mouse strain. They are a commonly used model organism in biomedical research.
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C57BL/6J is a mouse strain commonly used in biomedical research. It is a common inbred mouse strain that has been extensively characterized.
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BrdU is a synthetic nucleoside that is an analog of the DNA base thymidine. It can be incorporated into the newly synthesized DNA of replicating cells, substituting for thymidine during the DNA synthesis phase of the cell cycle.
More about "Corn oil"
Zea mays, vegetable oil, polyunsaturated fatty acids, linoleic acid, biodiesel, lubricants, PubCompare.ai, protocols, literature, preprints, patents, research optimization