L4-stage hermaphrodite worms were placed onto NGM plates containing seeded bacteria expressing dsRNA for each gene and were incubated for 36-40 h at 22°C or for 72 h at 15°C. Then, three worms were independently replica plated onto plates seeded with the same bacteria and were allowed to lay eggs for 24 h at 22°C before being removed. Progeny were scored for embryonic lethality after a further 24 h at 22°C, and post-embryonic phenotypes were scored blindly by two independent observers at the end of four successive 12-h intervals. Progeny laid on the first plate were also scored for post-embryonic phenotypes. A gene was found to be positive for a given phenotype if it could be observed in at least two of three worms or their progeny in at least two independent feeding experiments. Feeding times shorter than 36-40 h at 22°C or 72 h at 15°C were not always sufficient to produce a strong RNAi effect; for example, par-2 and par-3 were 53% and 23% embryonic lethal, respectively, after feeding for 24 h but were both 100% embryonic lethal after feeding for at least 36 h at 22°C (our unpublished results).
Eggs
Eggs are the reproductive bodies produced by female animals, particularly birds, reptiles, and some invertebrates.
They consist of an outer shell, membrane, and internal contents including the yolk and albumen.
Eggs provide essential nutrients and serve as a source of nourishment for the developing embryo.
Studying the properties and characteristics of eggs is crucial for understanding reproductive biology, nutrition, and developmental processes across a variety of species.
Reasearchers can leverage PubCompare.ai to quickly locate and compare the best egg research protocols from published literature, preprints, and patents, optimizing reproducibility and accuracy in their egg studies.
They consist of an outer shell, membrane, and internal contents including the yolk and albumen.
Eggs provide essential nutrients and serve as a source of nourishment for the developing embryo.
Studying the properties and characteristics of eggs is crucial for understanding reproductive biology, nutrition, and developmental processes across a variety of species.
Reasearchers can leverage PubCompare.ai to quickly locate and compare the best egg research protocols from published literature, preprints, and patents, optimizing reproducibility and accuracy in their egg studies.
Most cited protocols related to «Eggs»
Bacteria
Eggs
Embryo
Genes
Genes, Bacterial
Helminths
Hermaphroditism
Phenotype
RNA, Double-Stranded
RNA Interference
We created an overall plant-based diet index (PDI), a healthful plant-based diet index (hPDI), and an unhealthful plant-based diet index (uPDI). The procedure we used to create these indices is similar to the one used by Martínez-González et al. [13 (link)]; their “provegetarian food pattern” is similar in composition to our PDI. Frequencies of consumption of each food were converted into servings consumed per day. Then the number of servings of foods that belonged to each of 18 food groups were added up. The 18 food groups were created on the basis of nutrient and culinary similarities, within larger categories of animal foods and healthy and less healthy plant foods. We distinguished between healthy and less healthy plant foods using existing knowledge of associations of the foods with T2D, other outcomes (CVD, certain cancers), and intermediate conditions (obesity, hypertension, lipids, inflammation). Plant foods not clearly associated in one direction with several health outcomes, specifically alcoholic beverages, were not included in the indices. We also excluded margarine from the indices, as its fatty acid composition has changed over time from high trans fat to high unsaturated fat. We controlled for alcoholic beverages and margarine consumption in the analysis.
Healthy plant food groups included whole grains, fruits, vegetables, nuts, legumes, vegetable oils, and tea/coffee, whereas less healthy plant food groups included fruit juices, sugar-sweetened beverages, refined grains, potatoes, and sweets/desserts. Animal food groups included animal fats, dairy, eggs, fish/seafood, meat (poultry and red meat), and miscellaneous animal-based foods.
S1 Table details examples of foods constituting the food groups. The 18 food groups were divided into quintiles of consumption, and each quintile was assigned a score between 1 and 5. For PDI, participants received a score of 5 for each plant food group for which they were above the highest quintile of consumption, a score of 4 for each plant food group for which they were above the second highest quintile but below the highest quintile, and so on, with a score of 1 for consumption below the lowest quintile (positive scores). On the other hand, participants received a score of 1 for each animal food group for which they were above the highest quintile of consumption, a score of 2 for each animal food group for which they were between the highest and second highest quintiles, and so on, with a score of 5 for consumption below the lowest quintile (reverse scores). For hPDI, positive scores were given to healthy plant food groups, and reverse scores to less healthy plant food groups and animal food groups. Finally, for uPDI, positive scores were given to less healthy plant food groups, and reverse scores to healthy plant food groups and animal food groups. The 18 food group scores for an individual were summed to obtain the indices, with a theoretical range of 18 (lowest possible score) to 90 (highest possible score). The observed ranges at baseline were 24–85 (PDI), 28–86 (hPDI), and 27–90 (uPDI) across the cohorts. The indices were analyzed as deciles, with energy intake adjusted at the analysis stage.
Healthy plant food groups included whole grains, fruits, vegetables, nuts, legumes, vegetable oils, and tea/coffee, whereas less healthy plant food groups included fruit juices, sugar-sweetened beverages, refined grains, potatoes, and sweets/desserts. Animal food groups included animal fats, dairy, eggs, fish/seafood, meat (poultry and red meat), and miscellaneous animal-based foods.
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Alcoholic Beverages
Animals
Cereals
Coffee
Diet
Eggs
Fabaceae
Fats
Fats, Unsaturated
Fatty Acids
Feeds, Animal
Fishes
Food
Fowls, Domestic
Fruit
Fruit Juices
High Blood Pressures
Inflammation
Lipids
Malignant Neoplasms
Margarine
Meat
Nutrients
Nuts
Obesity
Plants
Plants, Edible
Red Meat
Seafood
Solanum tuberosum
Sugar-Sweetened Beverages
Vegetable Oils
Vegetables
Whole Grains
Zebrafish were maintained in accordance with UK Home Office regulations, UK Animals (Scientific Procedures) Act 1986, under project licence 80/2192, which was reviewed by The Wellcome Trust Sanger Institute Ethical Review Committee.
Heterozygous F2 fish were randomly incrossed and upon egg collection F2 adults were fin clipped and kept as isolated breeding pairs. For each family we aimed to phenotype 12 pairs, over 3 weeks of breeding. Each clutch of eggs, which was labelled with the breeding pair ID, was sorted into three 10cm petri dishes of ~50 embryos each. Embryos were incubated at 28.5°C. Previous mutagenesis screens were used as a reference for the phenotyping 27 (link),28 (link). Those phenotypes studied were: day 1 – early patterning defects, early arrest, notochord, eye development, somites, patterning and cell death in the brain; day 2 – cardiac defects, circulation of the blood, pigment (melanocytes), eye and brain development; day 3 – cardiac defects, circulation of the blood, pigment (melanocytes), movement and hatching; day 4 – cardiac defects, movement, pigment (melanocytes) and muscle defects; day 5 – behaviour (hearing, balance, response to touch), swim bladder, pigment (melanocytes, xanthophores and iridophores), distribution of pigment, jaw, skull, axis length, body shape, notochord degeneration, digestive organs (intestinal folds, liver and pancreas), left-right patterning. In the first round of the phenotyping, all phenotypic embryos were discarded. At 5 dpf, >48 phenotypically wild-type embryos were harvested. Embryos were fixed in 100% methanol and stored at −20°C until genotyping was initiated. In the second round, F2s that were heterozygous for a suspected causal mutation were re-crossed. All phenotypes observed in those clutches of embryos were counted, documented and photographed. Phenotypic embryos were fixed in 100% methanol and at 5 dpf 48 phenotypically wild-type embryos were also collected. The first round genotyping results were assessed using a Chi-squared test with a p-value cut off of <0.05. If the number of homozygous embryos was above the cut-off (i.e. in the expected 25% ratio), the allele was deemed to not cause a phenotype within the first 5 dpf. If the number of homozygous embryos was below the cut-off, the allele was carried forward into the second round of phenotyping. In the second round, we aimed to genotype 48 embryos for each phenotype, ideally from multiple clutches. An allele was documented as causing a phenotype if the phenotypic embryos were homozygous for the allele. We allowed up to 10% of embryos for a given phenotype to not be homozygous, to account for errors in egg collection. Such alleles were outcrossed for further genotyping with F4 embryos at a later date. Where possible, alleles were also submitted to complementation tests.
Heterozygous F2 fish were randomly incrossed and upon egg collection F2 adults were fin clipped and kept as isolated breeding pairs. For each family we aimed to phenotype 12 pairs, over 3 weeks of breeding. Each clutch of eggs, which was labelled with the breeding pair ID, was sorted into three 10cm petri dishes of ~50 embryos each. Embryos were incubated at 28.5°C. Previous mutagenesis screens were used as a reference for the phenotyping 27 (link),28 (link). Those phenotypes studied were: day 1 – early patterning defects, early arrest, notochord, eye development, somites, patterning and cell death in the brain; day 2 – cardiac defects, circulation of the blood, pigment (melanocytes), eye and brain development; day 3 – cardiac defects, circulation of the blood, pigment (melanocytes), movement and hatching; day 4 – cardiac defects, movement, pigment (melanocytes) and muscle defects; day 5 – behaviour (hearing, balance, response to touch), swim bladder, pigment (melanocytes, xanthophores and iridophores), distribution of pigment, jaw, skull, axis length, body shape, notochord degeneration, digestive organs (intestinal folds, liver and pancreas), left-right patterning. In the first round of the phenotyping, all phenotypic embryos were discarded. At 5 dpf, >48 phenotypically wild-type embryos were harvested. Embryos were fixed in 100% methanol and stored at −20°C until genotyping was initiated. In the second round, F2s that were heterozygous for a suspected causal mutation were re-crossed. All phenotypes observed in those clutches of embryos were counted, documented and photographed. Phenotypic embryos were fixed in 100% methanol and at 5 dpf 48 phenotypically wild-type embryos were also collected. The first round genotyping results were assessed using a Chi-squared test with a p-value cut off of <0.05. If the number of homozygous embryos was above the cut-off (i.e. in the expected 25% ratio), the allele was deemed to not cause a phenotype within the first 5 dpf. If the number of homozygous embryos was below the cut-off, the allele was carried forward into the second round of phenotyping. In the second round, we aimed to genotype 48 embryos for each phenotype, ideally from multiple clutches. An allele was documented as causing a phenotype if the phenotypic embryos were homozygous for the allele. We allowed up to 10% of embryos for a given phenotype to not be homozygous, to account for errors in egg collection. Such alleles were outcrossed for further genotyping with F4 embryos at a later date. Where possible, alleles were also submitted to complementation tests.
Adult
Air Sacs
Alleles
Animals
Blood Circulation
Body Shape
Brain
Brain Death
Cardiac Arrest
Cell Death
Cells
Cranium
Digestive System
Eggs
Embryo
Epistropheus
Fishes
Genetic Complementation Test
Genotype
Heart
Heterozygote
Homozygote
Hyperostosis, Diffuse Idiopathic Skeletal
Intestines
Liver
Melanocyte
Methanol
Movement
Muscle Tissue
Mutagenesis
Mutation
Notochord
Pancreas
Phenotype
Pigmentation
Somites
Touch
Zebrafish
Adult
Animals
Animals, Transgenic
Animals, Wild
Bacteria
Bath
Clone Cells
Dissection
Eggs
Microscopy, Fluorescence
RNA Interference
Strains
Young Adult
A. gambiae mosquitoes were maintained in standard insectary conditions (28°, 75–80% humidity, 12-hr/12-hr light/dark cycle). Larvae were raised in deionized water and fed finely ground TetraMin fish food. Embryo microinjection was performed essentially as described (Fuchs et al. 2013 (link); Pondeville et al. 2014 (link)). Freshly laid eggs were directly aligned against the edge of a nitrocellulose membrane kept wet with overlaying filter paper soaked with demineralized water. A mix of plasmids totaling 400 ng/µl of DNA (0, 1 mM NaHPO4 buffer pH 6.8, 5 mM KCl, 60 ng/µl helper plasmid, and generally 85 ng/µl of each of four distinct transgenesis plasmids) was injected under a Nikon Eclipse TE2000-S inverted microscope using an Eppendorf Femtojet injector and TransferMan NK2 micromanipulator. Injections were performed using the compensation pressure of the device, which was kept at 6000 hPa to promote a constant moderate flow of the DNA solution out of the quartz capillary. Microinjected eggs were left undisturbed on the injection slides, which were placed diagonally in a container with 1-cm-deep demineralized water, the part of the filter paper most distant from the eggs was dipped in water so that eggs remained wet by capillarity (Figure 1 ). Adult mosquitoes that survived microinjection were separated according to sex and crossed en masse to an excess of fresh wild-type adults. Neonate progeny larvae from several successive gonotrophic cycles were screened by spotting groups of 50–80 onto the wells of a 24-well teflon-coated diagnostic slide (Erie Scientific, Menzel GmbH, Braunschweig, Germany) under a Zeiss Axiovert 200M fluorescence microscope. When a fluorescent larva was detected, it was carefully isolated from the remainder larvae with the cut tip of a P200 pipette.
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Adult
Buffers
Capillaries
Capillarity
Culicidae
Diagnosis
DNA, A-Form
Eggs
Embryo
Fishes
Food
Humidity
Infant, Newborn
Larva
Medical Devices
Microinjections
Microscopy
Microscopy, Fluorescence
Nitrocellulose
Plasmids
Pressure
Quartz
Teflon
Tissue, Membrane
Most recents protocols related to «Eggs»
Example 20
Coffee cake was made from cake batter comprising the following ingredients:
The coffee cake was made in a glass cake pan into which the cake batter was placed and baked at ordinary temperature and time in an oven according to the recipe. The coffee cake was comparable or superior in quality and taste compared to coffee cake made using traditional all-purpose or cake flour and had superior nutritional profile. The coffee cake was lighter, fluffier, and more moist compared to conventional coffee cake made using all-purpose flour instead of the composite wheat-MCT flour.
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baking powder
Butter
Coffee
Cold Temperature
Eggs
Erythritol
Flour
Food
Hazelnuts
Light
Plants
Sodium Chloride
Taste
Vanilla
White Person
Example 8
Lung tissues from the 7 dogs were analyzed by quantitative real-time RT-PCR assays that detect the M gene of influenza type A and the H3 gene of canine H3N8 influenza A virus. The lungs from all 7 dogs were positive for both the influenza A M gene and the canine influenza H3 gene (Table 8). After 3 passages in MDCK cells, influenza A subtype H3N8 virus was isolated from the lungs of a shelter dog that died after 3 days of pneumonia. This virus was named A/canine/Jacksonville/05 (H3N8) (canine/Jax/05). After 2 passages in embryonated chicken eggs, influenza A subtype H3N8 virus was recovered from the lungs of the pet dog that also died after 3 days of pneumonia. This virus was named A/canine/Miami//05 (H3N8) (canine/Miami/05).
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Biological Assay
Canis familiaris
Chickens
Eggs
Genes
Influenza A virus
isolation
Lung
Madin Darby Canine Kidney Cells
Pneumonia
Pneumonia, Viral
Real-Time Polymerase Chain Reaction
Tissues
Virus
Virus Vaccine, Influenza
Example 5
This example describes the superior protection of plant comprising event MON 87411 from corn rootworm damage when compared to current commercial products (MON 88017 and DAS-59122-7) and negative control plants. Efficacy field trials were conducted comparing 135 plants each of event MON 87411, MON 88017, DAS-59122-7, and negative controls. Root damage ratings (RDR) were collected, and the percentage plants with an RDR less than the economic injury level (0.25 RDR) is shown in Table 8.
Table 8 shows that only about 4% of plants containing event MON 87411 exhibited RDRs greater than the economic threshold of 0.25 RDR. In contrast, 22% of the commercially available plants containing MON 88017 exhibited RDRs greater than the economic threshold of 0.25 RDR. And, 20% of the commercially available plants containing DAS-59122-7 exhibited RDRs greater than the economic threshold of 0.25 RDR. And, 96% of the negative control plants exhibited RDRs greater than the economic threshold of 0.25 RDR. The conclusion from these data is that event MON 87411 is clearly superior at providing protection from corn rootworm damage as compared to commercial products MON 88071 and DAS-59122-7, and a negative control.
Trial included 135 plants for each event tested.
Efficacy green house trials were conducted to test the performance of event MON 87411 with extreme infestation pressure of corn root worm. In this trial the following event were evaluated: event MON 87411, an event from transformation with DNA vector #890 expressing only the dsRNA; MON 88017; DAS-59122-7; and negative control. For these high-pressure efficacy trials, the corn plants under evaluation were grown in pots in a green house. Extreme infestation pressure was achieved by sequential infestation of each potted plant with approximately 2,000 WCR eggs per pot at their V2 growth stage, and, at 4 additional times occurring at 1 to 1½ week intervals with approximately 1,000 WCR eggs per pot per infestation for a total of approximately 6,000 WCR eggs added to each pot. Plant roots were removed, washed, and rated for RDR at their VT growth stage. The roots from all thirteen (N=13) negative control plants exhibited maximum root damage, or an absolute RDR of 3 RDR. These results illustrate that event MON 87411 is more superior to other corn events available for controlling corn rootworm (Table 9).
One measure of efficacy of corn rootworm transgenic events is by a determining the emergence of adult beetles from the potted soil of plants cultivated in a green house. To determine adult corn rootworm beetle emergence from the soil of event MON 87411 plants grown in pots, 10 to 15 plants were germinated in pots containing soil infested with WCR eggs, similar to that described above. Throughout the growth period, each corn plant was covered with mesh bag to contain any emerging adult beetles.
Counts of above ground adult beetles were made at 6, 12, and 18 weeks after plant emergence, and at the end of the trial the roots were evaluated for RDR. Plants containing event MON 87411 were compared to negative control plants, and other corn rootworm protective transgenic events. The results were that significantly fewer beetles were observed to emerge from soils in which event MON 87411 plants were potted compared to the other corn rootworm protective transgenic events, illustrating the superior properties of event MON 87411 to protect against corn rootworm damage.
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Adult
Animals, Transgenic
Beetles
Cloning Vectors
Eggs
Helminthiasis
Injuries
Parasitic Diseases
Plant Roots
Plants
Pressure
RNA, Double-Stranded
Zea mays
Example 21
Banana nut bread was made from cake batter comprising the following ingredients:
The banana nut bread was made in a greased metal bread pan into which the cake batter was placed and baked at ordinary temperature and time in an oven according to the recipe. The banana nut bread was comparable or superior in quality and taste compared to banana nut bread made using traditional all-purpose or cake flour and had superior nutritional profile. The banana nut bread was lighter, fluffier and more moist compared to conventional banana nut bread made using all-purpose flour instead of the composite wheat-MCT flour.
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Banana
Bicarbonate, Sodium
Bread
Eggs
Erythritol
Flour
Food
Light
Metals
Milk, Cow's
Pecans
Plants
Sodium Chloride
Taste
Vanilla
The primary objective of the study described herein was to evaluate the efficacy of a fipronil deer feed against I. scapularis and A. americanum ticks parasitizing white-tailed deer under pen conditions. The vector-host association and treatment concept are presented in Fig. 1 . Ixodes scapularis was selected because it is a vector of seven human pathogens, with the most notable being those causing Lyme disease [31 (link), 32 (link)]. Lyme disease is the most common vector-borne disease in the USA, occurring most frequently in the Northeast and Midwest of the USA, and is estimated to account for approximately 500,000 human cases per year [32 (link)–34 (link)]. Amblyomma americanum was selected because it is suspected to vector five or more disease agents transmissible to humans [35 (link)], and is also linked with southern tick-associated rash illness (STARI) [36 (link)] and red meat allergy [37 (link), 38 ].![]()
Vector-host association (
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Amblyomma americanum
BLOOD
Cloning Vectors
Deer
Eggs
Females
fipronil
Homo sapiens
Hyperemia
Ixodes scapularis
Lyme Disease
Odocoileus virginianus
pathogenesis
red meat allergy
Reproduction
Southern tick-associated rash illness
Ticks
Vector Borne Diseases
Woman
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
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Prism 6 is a data analysis and graphing software developed by GraphPad. It provides tools for curve fitting, statistical analysis, and data visualization.
More about "Eggs"
Eggs are the reproductive structures produced by female animals, particularly birds, reptiles, and some invertebrates.
These ovular bodies consist of an outer shell, membrane, and internal contents like the yolk and albumen.
Eggs provide essential nutrients and serve as a source of nourishment for the developing embryo.
Understanding the properties and characteristics of eggs is crucial for researchers studying reproductive biology, nutrition, and developmental processes across diverse species.
Leveraging tools like PubCompare.ai, researchers can quickly locate and compare the best egg research protocols from published literature, preprints, and patents.
This helps optimize reproducibility and accuracy in egg studies.
Researchers may also utilize common laboratory reagents and equipment like FBS, DMEM, TRIzol reagent, Penicillin, Streptomycin, TRIzol, Penicillin/streptomycin, SZX16, RNeasy Mini Kit, and Prism 6 to support their investigations.
Whether you're studying avian, reptilian, or invertebrate eggs, the insights gained can have far-reaching implications for reproductive science, developmental biology, and beyond.
By leveraging the latest tools and techniques, researchers can unlock new discoveries and advance our understanding of these remarkable biological structures.
These ovular bodies consist of an outer shell, membrane, and internal contents like the yolk and albumen.
Eggs provide essential nutrients and serve as a source of nourishment for the developing embryo.
Understanding the properties and characteristics of eggs is crucial for researchers studying reproductive biology, nutrition, and developmental processes across diverse species.
Leveraging tools like PubCompare.ai, researchers can quickly locate and compare the best egg research protocols from published literature, preprints, and patents.
This helps optimize reproducibility and accuracy in egg studies.
Researchers may also utilize common laboratory reagents and equipment like FBS, DMEM, TRIzol reagent, Penicillin, Streptomycin, TRIzol, Penicillin/streptomycin, SZX16, RNeasy Mini Kit, and Prism 6 to support their investigations.
Whether you're studying avian, reptilian, or invertebrate eggs, the insights gained can have far-reaching implications for reproductive science, developmental biology, and beyond.
By leveraging the latest tools and techniques, researchers can unlock new discoveries and advance our understanding of these remarkable biological structures.