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Sludge

Sludge refers to the semi-solid residue generated from industrial or municipal wastewater treatment processes.
It consists of a mixture of organic matter, pathogens, and other contaminants.
Proper management and optimization of sludge is crucial for environmental protection and resource recovery.
This MeSH term describes the characteristics, composition, and processing methods for sludge, including thickening, dewatering, and disposal.
Researchers can use AI-driven tools like PubCompare.ai to identify the most effective sludge optimization protocols from the literature, preprints, and patents, streamlining their research and maximizing its impact.
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Most cited protocols related to «Sludge»

Two mock communities were used to evaluate amplicon preparation, data quality, and quantitative biases. Both communities consisted of the same five 16S rRNA gene clones (H42, AF234715; H29, AF234692; H28, AF234749; H13, AF234737; H44, AF234743) from an activated sludge study (Juretschko et al., 2002 (link)) that were combined to have even or uneven proportions. Purified plasmids were quantified using the Qubit® dsDNA BR Assay Kit (Life Technologies) and a Qubit® 2.0 Fluorometer system (Life Technologies). For the even mock community the plasmids were mixed in equimolar proportions, for the uneven mock community the clones were mixed in a more realistic fashion, resulting in relative abundances of the individual clones at 76, 18, 5, 0.7, and 0.09%, respectively. After construction, the even and uneven mock communities were diluted to a final concentration of 0.1 ng μL−1.
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Publication 2015
Biological Assay Clone Cells DNA, Double-Stranded Plasmids Ribosomal RNA Genes Sludge
Three replicate samples were subjected to RNA extraction, using the RiboPure-Bacteria Kit (Thermo Fisher Scientific, USA) according to the protocol, except that the input was 2.2 mg TS activated sludge biomass, and bead beating (160s at 6 m/s) was performed with FastPrep FP120 (MP Biomedicals, USA). Extracted total RNA purity was evaluated with Nanodrop1000 (Thermo Scientific Fisher, USA), quality with Tapestation 2200, using High Sensitivity screentapes (Agilent, USA) and concentration, using Qubit RNA BR Assay Kit (Thermo Scientific Fisher, USA). The extracted RNA was used for library preparation, using the TruSeq stranded mRNAseq protocol (Illumina Inc., USA) according to the manufacturer’s recommendations and sequenced, using 2x75bp MiSeq Reagent kits v3 on an Illumina MiSeq. The reads were trimmed, using CLC genomics workbench v.7.03 (CLCbio, Qiagen), requiring a minimum Phred score of 20 and a minimum length of 75 bp. In addition, the reverse reads were discarded in order not to bias the subsequent count based analysis. The trimmed metatranscriptome reads were mapped to the MiDAS database version 1.20 [33 ], using the map reads to reference function in CLC genomics workbench v.7.03, requiring 95 percent similarity over the full read length and random assignments of reads, which mapped to two sequences equally well. The results were exported as.csv files, imported to R and converted to phyloseq objects for easy manipulation and visualization. See online documentation for the exact workflow applied.
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Publication 2015
Bacteria Biological Assay CLCN7 protein, human DNA Library DNA Replication GOLPH3 protein, human Hypersensitivity Sludge
Four publicly available metagenomes were selected for evaluation: a metagenome sampled from acid mine drainage (Tyson et al., 2004 (link), accession numbers AADL01000110.1-AADL01001068.1, CH003545.1-CH004435.1, DS995259.1-DS995275.1), one obtained from enhanced biological phospate removing (EBPR) sludge (Martin et al., 2006 (link), accession numbers AATN01000001.1-AATN01011188.1), one from an Olavius algarvensis microbial symbiont community (Woyke et al., 2006 (link), accession numbers AASZ00000000.1, DS021108.1-DS022223.1), and one from an Antarctic whale fall bone (Tringe et al., 2005 (link), accession numbers AAGA01000001.1-AAGA01026232.1). In addition, an artificial metagenome was used (SimBG, Saeed et al., 2011 (link)). All five metagenomes were also used for evaluation by Saeed et al. (2011 (link)). Additional information is provided in Table 1. For evaluation of the real metagenomes, we assumed that the annotations provided by the authors of the original studies were correct. In the EBPR case no annotations were provided, so we used the published genome of Candidatus “Accumulibacter phosphatis” as the reference. After binning, a bin was assigned to each population and the accuracy was calculated as the number of correctly binned nucleotides divided by the total number of nucleotides in the bin (×100%). Recall was calculated as the number of nucleotides of the source organism assigned to the bin divided by the total number of nucleotides of the source organism present in the metagenome (×100%). For the Whale Fall metagenome, evaluation of accuracy and recall was impossible, as binning was reported to be unsuccessful by the authors. The results (accuracy, recall, and computation time) were compared to two comparable previously published state of the art de novo compositional binners (Kelley and Salzberg, 2010 (link); Saeed et al., 2011 (link)). For SCIMM (Kelley and Salzberg, 2010 (link)), bins were seeded with a single trial of Likely Bin and the algorithm was run multiple times with different estimates for the number of populations. In Table 1 only the results for the optimal choice are shown. 2T binner was run with the default options.
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Publication 2012
Acids Biopharmaceuticals Bones Drainage Genome Mental Recall Metagenome Microbial Community Nucleotides Population Group Sludge Whales
MetAMOS v1.5rc3 was executed using default settings. MG data were provided as input for single-omic assemblies (MetAMOS_MG) while MG and MT data were provided as input for multi-omic co-assemblies (MetAMOS_MGMT). All computations using MetAMOS were set to use eight computing cores (“-p 8”).
MOCAT v1.3 (MOCAT.pl) was executed using default settings. Paired-end MG data were provided as input for single-omic assemblies (MOCAT_MG) while paired-end MG and MT data were provided as input for multi-omic co-assemblies (MOCAT_MGMT). All computations using MOCAT were set to use eight computing cores (“-cpus 8”). Paired-end reads were first preprocessed using the read_trim_filter step of MOCAT (“-rtf”). For the human fecal microbiome datasets (HF1–5), the preprocessed paired- and single-end reads were additionally screened for human genome-derived sequences (“-s hg19”). The resulting reads were afterwards assembled with default parameters (“-gp assembly -r hg19”) using SOAPdenovo.
IMP v1.4 was executed for each dataset using different assemblers for the co-assembly step: i) default setting using IDBA-UD, and ii) MEGAHIT (“-a megahit”). Additionally, the analysis of human fecal microbiome datasets (HF1–5) included the preprocessing step of filtering human genome sequences, which was omitted for the wastewater sludge datasets (WW1–4) and the biogas (BG) reactor dataset. Illumina TruSeq2 adapter trimming was used for wastewater dataset preprocessing since the information was available. Computation was performed using eight computing cores (“- -threads 8”), 32 GB memory per core (“- -memcore 32”) and total memory of 256 GB (“- -memtotal 256 GB”). The customized parameters were specified in the IMP configuration file (exact configurations listed in the HTML reports [57 ]). The analysis of the CAMI datasets were carried using the MEGAHIT assembler option (“-a megahit”), while the other options remained as default settings.
In addition, IMP was also used on a small scale dataset to evaluate performance of increasing the number of threads from 1 to 32 and recording the runtime (“time” command). IMP was launched on the AWS cloud computing platform running the MEGAHIT as the assembler (“-a megahit”) with 16 threads (“- -threads 16”) and 122 GB of memory (“- -memtotal 122”).
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Publication 2016
Biogas Feces Genome, Human Human Microbiome Memory O(6)-Methylguanine-DNA Methyltransferase Sludge Strains
The capability of sewage sludge microbial communities to utilize a variety of carbon sources was assessed by using Biolog EcoPlate [22 ]. Every plate had 96 wells containing 31 different carbon sources plus a blank well, in three replications. The rate of utilization of the carbon sources was pointed by the reduction of tetrazolium violet redox dye, which changed from colorless to purple if added microorganisms utilize the substrate [23 ]. EcoPlate was prepared in the following way: 1 g of sewage sludge was suspended in 99-ml sterile peptone water and shaken for 20 min at 20 °C and then was incubated at 4 °C for 30 min [24 (link)]. Next, each well of the Biolog EcoPlate was inoculated by 120 μl of the prepared suspension and incubated at 25 °C. Absorbance at 590 nm was measured on Biolog Microstation after 24, 48, 72, 96, 120, and 144 of incubation hours. Optical density (ODi) value from each well was corrected by subtracting the control (blank well) values from each plate well. Optical density values obtained at 120 h of incubation represented the optima range of optical density readings, so 120 h of incubation results was used for the assessment of microbial functional diversity and statistical analyses. In addition, substrates were subdivided into five group substrates, carbohydrates, carboxylic and ketonic acids, amines and amides, amino acids, and polymers, according to Weber and Legge [25 (link)].
Microbial activity in each microplate was expressed as average well color development (AWCD). Substrate richness values (R) were calculated as the number of utilized substrates and evenness were calculated according to Zak et al. [26 (link)] (Table 2).

Formulae for calculations

IndexDefinitionFormulaeDefinitions
Average well color developmentAWCD = Σ ODi/31

pi = proportional color development of the well over total color development of all wells of a plate

H = Shannon index of diversity

S = number of wells with color development (substrate utilization richness)

Shannon diversityMeasure of richnessH = −Σpi(lnpi)
Shannon evennessEvenness calculated from Shannon indexE = H/lnS
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Publication 2014
Acids Amides Amines Amino Acids Carbohydrates Carbon DNA Replication Ketones Microbial Community Oxidation-Reduction Peptones Polymers Sewage Sludge Sterility, Reproductive tetrazolium violet Vision

Most recents protocols related to «Sludge»

Bacillus subtilis of TLRI 211-1 was screened from the activated sludge at TLRI. After species identification, high temperature resistance (50°C), and spore-producing ability test, the B. subtilis was selected and inoculated in Tryptic Soy Broth culture media and placed in a 30°C incubator for 24 hours. After a process of adjusting with food grade silicon dioxide, mixing well, centrifuging to remove the upper liquid and air drying in a 40°C oven, the total content of viable sporulated TLRI 211-1 bacteria used in this experiment was 1×109 CFU/g. Commercial B. amyloliquefaciens (CML. B. amyloliquefaciens) used in the treatment 4 was provided by Yungstrong, Vetnostrum Animal Health Co., Ltd, (Hsinchu, Taiwan) and adjusted to the content of 1×109 CFU/g.
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Publication 2023
Animals Aptitude Tests Bacillus subtilis Bacteria Culture Media Fever Food Silicon Dioxide Sludge Spores tryptic soy broth
The preculture of algal flocs was created using activated sludge from an aeration tank cultured in secondary effluent in a batch reactor under static conditions. In this case, the biomass concentration was 694 mg L−1 after 30 days, and the mean diameter of algal flocs was 0.994 mm (Biliani and Manariotis 2022 ). The present study examined the formation of photogranules in six configurations (Fig. S1). In each case, 85 mL of the preculture was placed in a 1-L glass beaker, and a final volume of 1000 mL was reached with primary effluent wastewater (PE) (3 reactors) or secondary effluent (SE) (3 reactors). Every reactor had different mixing and fill-draw conditions, as shown in Table S1. The six reactors were placed in a room next to the window at a temperature of 20 ± 5 °C for 240 days. Primary and secondary effluent was sampled from the wastewater treatment plant (WWTP) of the University of Patras campus at Rio, and their characteristics are given in Table 1. Mixing was not used in the static reactors (PE Static and SE Static), while mixing at 30 rpm was applied in the rest of the reactors (PE Mixing 1, PE Mixing 2, SE Mixing 3 and SE Mixing 4). During the 2nd period, the hydraulic reaction time (HRT) was doubled from 8 to 16 days in the PE Static and PE Mixing 1 reactor, 4 to 8 days PE in the Mixing 2 reactor, 2 to 4 days in the SE Static and SE Mixing 3 reactor and 1.3 to 2.7 days in the SE Mixing 4 reactor. In the 3rd period, the volume withdrawn decreased to half in the SE cultures but remained the same in the PE cultures. In the 4th period, the mixing velocity increased from 30 to 50 rpm for the mixing cultures (PE Mixing 1, PE Mixing 2, SE Mixing 3 and SE Mixing 4) and the HRT of the SE cultures was decreased to half. The light intensity in the 4th period increased by adding led lamps (12 V, RGB SPECTRUM, USA) with a photoperiod of 24 h yielding an irradiance of 100 μmol photons m−2 s−1, compared to 50 photons m−2 s−1 during the 3rd period. In the 5th period, the HRT was further decreased in the SE but remained the same in the PE cultures. The operation cycle consisted of feeding (10 min), rapid mixing (2 min), reaction (0.98 to 1.98 days), mixing (2 min), settling (10 min) and decanting (10 min). Samples were systematically taken from the supernatant at the end of the settling phase. Less frequently, samples were taken from the mixed liquor before the settling phase.

Characteristics of wastewater and microalgae preculture

NutrientUnitsPrimary effluentSecondary effluentMicroalgae Preculture
RangeMeanSD*RangeMeanSDMeanSD
CODmg L−1200–2202051050–60545--
TSSmg L−130–4033520–302152002.6
Chl-amg m−3------5970.002
Total-Pmg L−14.5–5.55.00.53.5–5.54.20.757.80.1
NO3-Nmg L−10.0–1.10.570.55.5–127.93.250.40.06
NH3-Nmg L−110–3022102.0–105.344.70.03
pH-7.0–8.07.70.57.0–8.07.40.58.9-

*SD: standard deviation

Publication 2023
AMG 3 Amniotic Fluid Hypomenorrhea Microalgae Plants Sludge
The micron-sized monodisperse SiO2 microspheres were prepared according to the literature methods (Xing, 2015 ). CTS and PLA were purchased from Aladdin Chemical Reagent Co. (Shanghai, China; A. R. grade, purity ≥98%). Toluene, carbon disulfide, N,N-dimethylformamide, and catechol were bought from Yantai Yuandong Fine Chemical Co., Ltd. (Yantai, China; A. R. grade, purity ≥98%).
The microorganism used in this study was Bacillus stercoris EGI312, which was isolated and purified from activated sludge. The activated sludge was taken from the sewage treatment station of Shandong Chambroad Petrochemicals Co., Ltd. in Binzhou, Shandong, China. The bacteria were cultured in fresh LB medium for 2–3 days to an optical density (OD) of 2. After being centrifuged at 5,000 rpm for 5 min, the bacteria were resuspended with 0.9% NaCl for immobilization according to the method by Deng et al. (2017) (link).
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Publication 2023
Bacillus subtilis subsp. stercoris Bacteria Carbon disulfide catechol Color Vision Culture Media Dimethylformamide Immobilization Microspheres Normal Saline Sewage Sludge Toluene
The apparatus used for nitrate removal experiment is shown in Figure 1. Each experimental group included a column reactor, peristaltic pump, inlet pool (180 L) and outlet pool (10 L). The column reactor was made of completely transparent acrylic sheet, with an internal diameter of 50 mm and a height of 900 mm, and the reactor was filled with 1.2 L wastewater without adding carbon source material. The reactor was divided into a water distribution layer, a support layer (pore diameter of 3 mm, to prevent carbon sources from falling into the water distribution layer), and filling layer from bottom to top, with four outlets on the column. The experimental inlet water was artificially simulated by recirculating mariculture wastewater with a salinity of 31 ± 1 ‰, NO3-N concentration of 30 mg/L (adjusted by KNO3), phosphate (PO43−-P) concentration of 1 mg/L (adjusted by KH2PO4), pH of 7.0–7.5 (adjusted by HCl), and the temperature was controlled at 25 ± 1°C during the experiment.
In order to improve the membrane hanging start-up efficiency of the reactor, the denitrifiers adapted to high salinity were acclimated and enriched before the experiment, and detailed steps are as follows: solid wastes discharged from marine recirculating aquaculture system were used as seeding sludge and inoculated into nutrient solution (30 mg/L NO3-N, 1 mg/L PO43−-P, salinity 32‰), and reached mixed liquor suspended solids (MLSS) for 3 g/L. MLSS concentrations were measured according to the Standard Methods (APHA et al., 2012 ). Before the experiment, four types of carbon source materials were added into the reactor, and then seeding sludge was evenly inoculated into the column reactor. The carbon source fill rate was 50% and the hydraulic retention time (HRT) was set to 5 h. The NO3-N and NO2-N content in the outlet were sampled and measured, and the start-up operation finished as the biofilm reactor reached a steady-state with the effluent NO3-N less than 1.5 mg/L, and this process lasts about 2 months.
The experimental phase was 180 h. The wastewater in the inlet pool was pumped into the inlet through a peristaltic pump and discharged from the outlet to the outlet pool through the reactor. The NO3-N, NO2-N, NH4+-N, total nitrogen (TN), and COD concentrations at the outlet were measured every 8 h. Water samples were filtered first using a 0.45 μm membrane, and then NO3-N, NO2-N, TN (no membrane filtration), and NH4+-N were determined using an automatic nutrient analyzer (QuAAtro, SEAL, Germany). The nitrate removal efficiency (NRE) was calculated as follows:
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Publication 2023
Amniotic Fluid Biofilms Carbon Filtration Marines Nitrates Nitrogen Nutrients Peristalsis Phocidae Phosphates Retention (Psychology) Salinity Sludge Tissue, Membrane
The content of metals in the sludges was determined using Inductively Coupled Plasma Optical Emission Spectrometry ICP-OES according to EN ISO 11885:2009 (Optima 5300DV, PerkinElmer, USA) after prior mineralization of the sludges in aqua regia. The content of Cd, Ni, Cu, Cr, Co, and Pb in aqueous solutions after the leaching experiment was determined using inductively coupled plasma mass spectrometry ICP-MS according to EN ISO 17294–2:2016 (NexION 300S, PerkinElmer, USA); the determination of metals in the obtained solutions was performed with the level of uncertainty of 15%, a coverage factor of 2, and a significance level of 95%, and the limit of quantification (LOQ) for each element reached 0.02 mg/L.
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Publication 2023
A-factor (Streptomyces) aqua regia factor A Mass Spectrometry Metals Physiologic Calcification Plasma Sludge Spectrometry Vision

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More about "Sludge"

Sludge, also known as biosolids, is a semi-solid byproduct generated from wastewater treatment processes.
It consists of a mixture of organic matter, pathogens, and other contaminants.
Proper management and optimization of sludge is crucial for environmental protection and resource recovery.
Sludge can be thickened, dewatered, and disposed of through various methods.
Researchers can use advanced analytical tools like the Qubit fluorometer or the MiSeq sequencing platform to study the composition and characteristics of sludge.
Techniques such as the FastDNA SPIN Kit for Soil, the PowerSoil DNA Isolation Kit, or the DNeasy PowerSoil Kit can be used to extract and purify DNA from sludge samples for further analysis.
The E.Z.N.A.® soil DNA Kit is another tool that can be utilized to extract high-quality DNA from sludge.
The extracted DNA can then be quantified using the Qubit 2.0 Fluorometer, which provides accurate and sensitive measurements.
Researchers can also use AI-driven tools like PubCompare.ai to identify the most effective sludge optimization protocols from the literature, preprints, and patents.
This can help streamline their research and maximize its impact, ultimately leading to improved sludge management and resource recovery.
Sludge optimization is a crucial aspect of wastewater treatment, and the use of advanced analytical tools and AI-driven technologies can help researchers and practitioners enhance the efficiency and sustainability of sludge management.
Experince the futrue of sludge optimization today.