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Chemical content

Chemical content refers to the composition and properties of chemical substances, including their molecular structure, physical characteristics, and reactivity.
This term encompasses the study of organic and inorganic compounds, as well as the analysis and identification of chemical components in various materials and samples.
Chemical content is a fundamental aspect of chemistry, biology, and other scientific disciplines, and is essential for understanding the behavior and interactions of matter at the molecular level.
Reserach in this area can lead to advancements in areas such as drug development, material science, and environmental protection.

Most cited protocols related to «Chemical content»

Generation rate, composition, and physical and chemical nature of both feces and urine were recorded as of Table 1. Each recorded datum was the mean of the data from the reported study. Some published papers reported two or more independent studies so these papers contributed more than one value to the data set. The mean and median of each variable were both calculated as measures of central tendency and data were checked for normality by calculating a coefficient of skewness (Young, 1962 ):


Measured variables for feces and urine
 Feces unitUrine unit
Variableof measureof measure
Generationg/cap/dayL/cap/day
Frequency of defecationmotions/24 hrurinations/24 hr
Water content% total mass% total mass
Organic composition% total mass% dry mass
Components of solids% total mass% total mass
Inorganic composition% dry mass% dry mass
Daily excretion of elementsg/cap/dayg/cap/day, mg/L
Chemical nature  
 pHpHpH
 COD and BODmg/g wet massmg/L
Physical form  
 Bristol stool formLinear scale (1–7) 
 Diarrhea prevalence% of population 
σ = Standard deviationn = Valid number of casesBox and whisker plots were created using Statistica 11 software (Statsoft Inc., Tulsa, OK, USA, 2011). Outliers of each data set were defined using a standard default outlier coefficient value (Burns et al., 2005 (link)).

No outliers were removed from the data set but were identified in the graphical output. Full statistical calculations were only conducted on variables that had at least seven values but a median value is given for data when there were less than seven values.
A summary of studies used in the statistical analysis are outlined in Table 2, including the location and number of studies. A large proportion (80%) of the data set was from studies conducted in Europe and North America. A distinction was therefore made between low and high income countries by the measure of development; using the Human Development Index (HDI), a composite index measuring average achievement in three basic dimensions of human development; life expectancy, education, and income (UNDP, 2011 ).
The geographical location and human development index ranking of studies used in statistical analysis
CountrynHDI*References
Africa23/4aCranston and Burkitt (1975 (link)), Burkitt et al. (1980 (link))
Australia21Birkett et al. (1996 (link)), Hovey et al. (2003 (link))
Burma14Myo-Kin et al. (1994 (link))
Canada31Burkitt et al. (1980 (link)), Vuksan et al. (1999 (link))
China32Jie et al. (2000 (link)), Chen et al. (2008 (link)), Bai and Wang (2010 )
Denmark21Maclennan and Jensen (1977 (link)), Jensen et al. (1982 (link))
Developing countries23/4aFeachem et al. (1978 )
Europe and North America11/2bFeachem et al. (1978 )
European11bMykkänen et al. (1998 (link))
Finland41Reddy et al. (1975 (link)), Reddy et al. (1978 (link)), Jensen et al. (1982 (link)), Mykkänen et al. (1998 (link))
Germany11Erhardt et al. (1997 (link))
Guatemala13Calloway and Kretsch (1978 (link))
Holland41Stasse-Wolthuis et al. (1980 (link)), Van Faassen et al. (1993 (link)), Gaillard (2002 ), Wierdsma et al. (2011 (link))
India13Shetty and Kurpad (1986 (link))
Iran12Adibi et al. (2007 (link))
Japan71Glober et al. (1977 ), Polprasert and Valencia (1981 ), Tarida et al. (1984 (link)), Saitoh et al. (1999 (link)), Danjo et al. (2008 (link)), Shinohara et al. (2010 (link)), Hotta and Funamizu (2009 (link))
Kenya14Cranston and Burkitt (1975 (link))
New Zealand11Pomare et al. (1981 )
North America11bVuksan et al. (2008 (link))
Peru12Crofts (1975 (link))
Singapore11Chen et al. (2000 (link))
South Africa23Burkitt et al. (1972 ), Walker (1975 (link))
Spain11Roig et al. (1993 (link))
Sweden41Reddy et al. (1978 (link)), Vinneras (2002 ), Vinnerås et al. (2006 )
Thailand22Danivat et al. (1988 ), Schouw et al. (2002 (link))
Tonga12Pomare et al. (1981 )
UK261Olmsted et al. (1934 ), Connell et al. (1965 (link)), Southgate and Durnin (1970 (link)), Burkitt et al. (1972 ), Goy et al. (1976 (link)), Wyman et al. (1978 (link)), Prynne and Southgate (1979 (link)), Stephen and Cummings (1980 (link)), Eastwood et al. (1984 (link)), Eastwood et al. (1986 (link)), Davies et al. (1986 (link)), Cummings et al. (1987 (link)), Sandler and Drossman (1987 (link)), Cummings et al. (1992 (link)), Murphy et al. (1993 (link)), Cummings et al. (1996 (link)), Lewis and Heaton (1997 (link)), Chen et al. (1998 (link)), Reddy et al. (1998 (link)), Rivero-Marcotegui et al. (1998 (link)), Aichbichler et al. (1998 (link)), Almeida et al. (1999 ), Magee et al. (2000 (link)), Chaplin et al. (2000 (link)), Woodmansey et al. (2004 (link)), Silvester et al. (2011 (link))
USA181Canfield et al. (1963 ), Watts et al. (1963 (link)), Diem and Lentner (1970 ), Goldsmith and Burkitt (1975 (link)), Cummings et al. (1978 ), Glober et al. (1977 ), Goldberg et al. (1977 (link)), Beyer and Flynn (1978 (link)), Reddy et al. (1978 (link)), Calloway and Kretsch (1978 (link)), Kien et al. (1981 (link)), Polprasert and Valencia (1981 ), Tucker et al. (1981 (link)), Schubert et al. (1984 (link)), Parker and Gallagher (1988 ), Zuckerman, et al. (1995 (link)), Aichbichler et al. (1998 (link)), McRorie et al. (2000)

*Human Development Index Classifications (UNDP, 2011 ): 1. Very high, 2. High, 3. Medium, 4. Low.aClassification not available, presumed to be ranking 3 or 4.bClassification not available, presumed to be ranking 1 or 2.

Preliminary data analysis indicated that fiber intake was a major cause of variation in fecal generation and composition. There were a sufficient number of studies that had examined the effects of fiber intake on fecal output to enable further analysis to be undertaken on these data. The total dietary fiber intake was related to the generation of feces in linear and nonlinear regression analyses.
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Publication 2015
A TSQ Quantum Ultra Plus triple-quadrupole mass spectrometer (Thermo Fisher Scientific, San Jose, CA) equipped with an automated nanospray apparatus (i.e., Nanomate HD, Advion Bioscience Ltd., Ithaca, NY) and Xcalibur system software were utilized in the study13 (link). Ionization voltages of -1.1, -0.95, and +1.2 kV and gas pressures of 0.3, 0.15, and 0.3 psi were employed on the nanomate apparatus for the analyses of anionic lipids, PE, and PC, respectively. The nanomate was controlled by Chipsoft 7.2.0 software. Each lipid extract solution prepared above was diluted to less than 50 pmol of total lipids/μl with CHCl3/MeOH/isopropanol (1:2:4 by volume) prior to infusion to the mass spectrometer with the nanomate. This concentration was estimated based on the protein content and the total lipid content that is normalized to protein content from prior studies or previous experience. This procedure uses low concentrations of lipid to prevent lipid aggregation during analysis and to minimize any effects of residual inorganic components carried over during lipid extraction on ion suppression and/or chemical noise.
The first and third quadrupoles were used as independent mass analyzers with a mass resolution setting of 0.7 Thomson while the second quadrupole served as a collision cell for tandem mass spectrometry. Typically, a 1 to 2-min period of signal averaging in the profile mode was employed for each full MS scan. For tandem mass spectrometry, a collision gas pressure was set at 1.0 mTorr but the collision energy varied with the classes of lipids as indicated or described previously3 (link), 7 (link). For each tandem MS mass spectrum, a 2 to 5-min period of signal averaging in the profile mode was employed. All the full MS scans and tandem MS scans were automatically acquired by a customized sequence subroutine operated under Xcalibur software. Data from biological samples were normalized to the protein content and all data are presented as the mean ± SD of multiple samples from different animals.
Publication 2009
Animals Biopharmaceuticals Cells Chloroform Isopropyl Alcohol Lipids Pressure Proteins Radionuclide Imaging Tandem Mass Spectrometry
Two 3T whole-body Siemens Tim Trio (Siemens Medical Solutions, Erlangen, Germany) scanners were used in this study; one located at the Center for Magnetic Resonance Research (CMRR) in Minnesota and the other one at the Institut du Cerveau et de la Moelle (ICM) in Paris. Age- and BMI-matched healthy subjects (Table 1) were enrolled after giving informed consent according to procedures approved by the Institutional Review Board at CMRR and by the local ethics committee at ICM. The standard body RF coil was used for radiofrequency transmission and the 32-channel phased-array Siemens head coil was used for signal reception. Soft pads were used to hold each subject’s head in place to minimize head movement in the MR system. T1-weighted MPRAGE images (repetition time (TR) = 2530ms, echo time (TE) = 3.65 ms, flip angle = 7°, slice thickness = 1 mm, 224 slices, field-of-view = 256×176 mm2, matrix size = 256×256) were acquired to position the volume-of-interest (VOI) for MRS measurements. B0 shimming was achieved using an adiabatic version of FAST(EST)MAP (16 (link)), which is available as a work-in-progress (WIP) package on the Siemens system.
Proton spectra were acquired using a modified semi-LASER sequence (TE = 28 ms, TR = 5 s, 64 averages) (14 (link)) from two VOIs: cerebellar vermis (10×25×25 mm3) and pons (16×16×16 mm3). Voxel placement was based on anatomical landmarks. The fourth ventricle, cervical spinal cord and the brainstem were used to separate the cerebellum. The surfaces, lobes, lobules and fissures of the cerebellum were then used as landmarks in positioning the voxel in the vermis. For pons VOI placement, the midbrain, fourth ventricle and the medulla were used as landmarks.
The semi-LASER sequence (14 (link)) used in this study is a more compact version of the originally published semi-LASER sequence (17 (link)). Briefly, the sequence consisted of a 2 ms asymmetric slice-selective 90° pulse (18 (link)) followed by two pairs of slice selective adiabatic full passage (AFP) pulses (4 ms duration, HS4 modulation, R25) (19 ), which were interleaved, rather than applied sequentially, to improve suppression of unwanted coherences with shorter spoiler gradient pulses. Water suppression was achieved with VAPOR, which was interleaved with outer volume suppression (OVS) to suppress unwanted coherences (18 (link)). A substantially lower chemical shift displacement error is obtained with the semi-LASER sequence (3.6% /ppm for the slice-selective 90° pulse and 2% /ppm for the AFP pulses) compared to the standard PRESS sequence provided on the Siemens platform (12–13% /ppm).
B1 levels required for localization pulses and for water suppression were adjusted for each voxel. Specifically, the RF power magnitude for the 90° asymmetric pulse was calibrated by monitoring the signal intensity whilst increasing the RF power and choosing the RF power setting that produced the maximum signal. The power for the AFP pulses was automatically set relative to the 90° pulse. A similar procedure was carried out for the water suppression calibration.
On the scanner, signals from individual coil elements were combined after correcting for phase shifts between elements and weighting them based on the coil sensitivities (20 (link)) to generate a free induction decay (FID). Each FID was then individually saved for shot-to-shot frequency and phase correction before averaging. Two non-suppressed water spectra were acquired: one for eddy current correction (the RF pulses of the VAPOR scheme were turned off) and one for use as reference for metabolite quantification (VAPOR and OVS schemes turned off in order to eliminate magnetization transfer effects). To evaluate the cerebrospinal fluid (CSF) contribution to each VOI, fully relaxed unsuppressed water signals were acquired at different TE’s ranging from 28–4000 ms (TR = 15 s) with the entire VAPOR and OVS scheme turned off (21 ).
All spectral processing was performed in Matlab by the same person prior to LCModel fitting. Eddy current correction was carried out first to correct for distorted line shapes and zero-order phase. Individual shots affected by subject motion (based on water suppression efficiency) were removed. Single-shot frequency correction was performed using a cross-correlation algorithm and phase correction was performed using a least-square fit algorithm. All steps were completely automated except for the removal of FIDs affected by motion. Finally the summed spectrum was referenced based on NAA resonance at 2.01 ppm.
Spectra were then analyzed with LCModel (22 (link)) with the water scaling option (version 6.3-0G). The model basis set was generated based on density matrix formalism as described before (23 (link)). The basis set also included macromolecule spectra, which were acquired using inversion-recovery technique in 4 healthy subjects (total averages = 928, TR = 2.5 s, inversion time, TI = 0.75 s, VOI = 15.6 mL, 5 ms duration HS5 inversion pulse, occipital cortex). Due to the shorter T1 relaxation time of the methylene protons of tCr at 3.93 ppm relative to other metabolite protons (24 (link)), this resonance was present in the metabolite-nulled macromolecule spectra and was removed using a Hankel singular value decomposition (HSVD) algorithm in Matlab. A 12.5 Hz Gaussian line broadening was also applied to the macromolecule spectra after incorporating a reference peak at 0 ppm (see supplementary material). No baseline correction, zero-filling or apodization functions were applied to the in vivo data prior to the analysis. LCModel fitting (supplementary material) was performed over the spectral range from 0.5–4.2 ppm.
Metabolite concentrations were determined after correcting for tissue water content and CSF contributions in the selected VOI using the water-scaling option in LCModel. The transverse relaxation times (T2) of tissue water and % CSF contribution to the VOI were obtained by fitting the integrals of the unsuppressed water spectra acquired in each VOI at different TE values with a bi-exponential fit (21 ), with the T2 of CSF fixed at 740 ms based on measurement of T2 of water in a small voxel located in ventricles with the same semi-LASER sequence (4 healthy subjects, TR = 15 s, VOI = 0.125–0.360 mL, twelve TE values ranging from 28–4000 ms), and three free parameters: T2 of tissue water, amplitude of tissue water, and amplitude of CSF water.
In order to obtain accurate metabolite concentrations, corrections must be made for T2 relaxation of both water and metabolites. In the case of semi-LASER, T2 relaxation is slowed due to the Carr-Purcell (CP) conditions, and T2 values under CP conditions must be used for quantification. For water, these values can be estimated by correcting the free precession T2 value measured for the tissue water signal at different echo-times by a fixed factor to account for CP effects. A previous study compared water T2 values measured with LASER and CP-LASER sequences at 4T and 7T (25 (link)). Extrapolating from that study, we assumed that the T2 of water under CP conditions is 1.5× longer than the measured free precession T2 at 3T. Signal loss due to T2 relaxation of metabolites was neglected since the apparent T2 is sequence-dependent. This assumption is justified by the fact that metabolites have longer T2 such that correction factors would be small at TE = 28 ms. Nonetheless this choice will result in somewhat underestimated metabolite concentrations relative to the true concentrations in tissue. A water content of 82% and 72% was used for vermis and pons, respectively (26 ,27 ).
Metabolites that were quantified with Cramér-Rao lower bounds (CRLB) ≤ 50% from at least half of the spectra from a particular brain region were included in the neurochemical profile. In addition, if the correlation between two metabolites was very high (i.e. correlation coefficient r more negative than −0.7) in the majority of the spectra from a region, then only their sum was reported, e.g. tCr (creatine + phosphocreatine) and tCho (glycerophosphorylcholine + phosphorylcholine). If there was indication for pairwise correlation with r from −0.5 to −0.7, then the concentration sum of the pair was reported in addition to the individual metabolites’ concentrations, e.g. NAA, NAAG and total NAA (tNAA, NAA + NAAG), as recommended by the LCModel manual, Jan 2013 (22 (link)). Moreover, spectra with the associated water reference linewidth greater than 10 Hz were excluded due to trends observed in overestimating aspartate and ascorbate and underestimating glutamate in these spectra. Water linewidths > 10 Hz only occurred for spectra acquired from the pons region.
Publication 2014
The reconstruction process has also been previously outlined (Feist et al, 2006 (link); Reed et al, 2006a (link)). Here, we provide certain details specific to this work. Starting from the metabolic network for iJR904 (Reed et al, 2003 (link)), additional reactions were added to the network based on E. coli-specific biochemical characterization studies (see Supplementary information for a complete list of references) and other reactions were removed (see Results). This process was aided by comparing the content of iJR904 with the EcoCyc database (see below). The E. coli genome annotation (Riley et al, 2006 (link)) was used as a citation source for biochemical characterization studies and a framework upon which translated metabolic proteins, and subsequently reactions, were assigned to form gene to protein to reaction (GPR) assignments. The SimPheny™ (Genomatica Inc., San Diego, CA) software platform was used to build the reconstruction. For each reaction entered into the reconstruction, the involved metabolites were characterized according to their chemical formula and charge determined using their pKa value for a pH of 7.2. Metabolite charge was determined using its pKa value(s). When the metabolite pKa was not available, charge was determined using the pKa of ionizable groups present in a metabolite (http://www.chemaxon.com/product/pka.html). All of the reactions entered into the network were designated as enzymatically catalyzed reactions or spontaneous reactions, were both elementally and charged balanced and are either reversible or irreversible. Reversibility was determined first from primary literature for each particular enzyme/reaction, if available (see Supplementary information for references). Additionally, general heuristic rules, like those applied by Kümmel et al (2006b) , were used to enter reversibility using knowledge about the physiological direction of a reaction in a pathway (sometimes including regulatory knowledge) and/or basic thermodynamic information (such as reactions hydrolyzing high-energy phosphate bonds are almost always irreversible). Furthermore, a thermodynamic analysis of reversibility was utilized to assign the directionality of some reactions (see above).
Publication 2007
Enzymes Escherichia coli Gene Products, Protein Genome Metabolic Networks Phosphates physiology Proteins Reconstructive Surgical Procedures

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Publication 2014

Most recents protocols related to «Chemical content»

Not available on PMC !
Measurement of chemical content was carried out to determine the chemical content due to the influence of treatment materials and storage time. Measurements were carried out using the Lactostar (c) 2008 Funke Gerber, chemical content which includes; fat, solid non-fat, protein, and lactose.
Publication 2024
To determine the chemical content of the formulations, samples of 1 cm in diameter were cut and dissolved in 1 mL of ethanol, later transferred to a volumetric flask of 25 mL, and the solution was read by UV–Vis spectrophotometry (Cary 100 Varian, Santa Clara, CA, USA) at a wavelength of 243 nm [17 (link)].
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Publication 2024
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The seasonal water dynamics in the substrate of all treatments was controlled using a data logger and sensors for the measurement of the volumetric water content (VWC) in the soil (Spectrum Technologies, Inc., Aurora, CO, USA). In each treatment, there were four sensors installed at a depth of 5-20 cm. Sampling for the substrate chemical trait determination was carried out in mid-September 2021 (six months after the P fertilization). In each box (treatment), one composite sample was collected, formed with nine subsamples collected in a diagonal (X) arrangement. The samples of each treatment were subjected to soluble component extraction by mixing with deionized water at a 1:2 ratio. The material was mixed for 1 h using a rotating mixer. Then, the suspension was filtered through filter paper, and the clear filtrate was analyzed to determine the pH reaction, total nitrogen (N total), nitrate (NO 3 -), ammonium (NH 4 + ), phosphorus (PO 4 3-), potassium (K + ), calcium (Ca 2+ ), magnesium (Mg 2+ ), chloride (Cl -) and sodium (Na + ) ions and salt, as well as electrical conductivity (E.C.), in the substrate of each treatment separately. The chemical analysis methodology applied is described in more detail in Page et al. [74] .
Publication 2024
To obtain the chemical composition of the cyanobacterium, the amounts of total nitrogen (N), nitrite (NO2), nitrate (NO3), ammonium (NH4+), sulfate (SO42−), phosphate (PO43−), carbonate (CO32−), magnesium (Mg2+), calcium (Ca2+), sodium (Na+), and potassium (K+) ions were determined at the Institute of Arian Fan Azma, Iran, following the methods summarized in Table 2. Each test was carried out in three replications, and the averages of the obtained amounts were provided.

Chemical content of cyanobacteria

Analytical MethodNostoc spongiaeforme ISB65
Total N (mg.L− 1)Macro kjeldahl35.00
NO2 (mg.L− 1)Colorimetric0.60
NO3 (mg.L− 1)Ultraviolet Spectrophotometric9.40
NH4+ (mg.L− 1)Nesslerization5.00
Phosphate (mg.L− 1)Vanadomolybdophosphoric acid colorimetric9.00
CO32− (mg.L− 1)Titrimetric0.00
HCO3 (mg.L− 1)Titrimetric30.00
Na+ (mg.L− 1)Flame Emission Photometric1.50
Mg2+ (mg.L− 1)EDTA Titrimetric5.00
Ca2+ (mg.L− 1)EDTA Titrimetric7.00
K+ (mg.L− 1)Flame Emission Photometric0.11
EC (µS cm− 1)Platinum Electrode15.00
pHElectrometric6.02
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Publication 2024
We obtained cross sections of leaves from the choice experiments using a razor blade, which was cleaned with ethanol before and after each use. Samples were fixed on an aluminum SEM mount covered with conductive carbon adhesive tape. The elemental composition of the samples was analyzed using a scanning electron microscope (SEM) (JSM-IT700HR) with an energy-dispersive X-ray spectrometer (EDX) (JED-2300 Analysis Station Plus, JEOL, Tokyo, Japan) at a low vacuum (30 Pa), 15 kV accelerating voltage, and 500× magnification. We measured three points within the epidermis of both the adaxial and abaxial surfaces and four points within the mesophyll. The SEM–EDX analysis was conducted on the same day as the toughness measurements.
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Publication 2024

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More about "Chemical content"

Chemical composition and properties are fundamental aspects of chemistry, biology, and other scientific disciplines.
This encompasses the study of organic and inorganic compounds, as well as the analysis and identification of chemical components in various materials and samples.
Key areas within chemical content include molecular structure, physical characteristics, and reactivity.
Researching chemical content can lead to advancements in diverse fields such as drug development, material science, and environmental protection.
Common chemical substances relevant to this topic include sodium hydroxide, triglycerides, thiobarbituric acid reactive substances (TBARS), methanol, gallic acid, hydrochloric acid, bovine serum albumin, and various chemical assay kits.
Techniques for analyzing chemical content may involve spectroscopic methods, chromatography, colorimetric assays, and other analytical approaches.
Accurate and reproducible chemical research is enabled by tools that help identify the most reliable experimental protocols from literature, preprints, and patents.
By understanding the fundamentals of chemical composition and properties, researchers can unlock new insights and innovations at the molecular level, driving progress across the sciences.