Log In Sign up
The largest database of trusted experimental protocols
> Physiology > Cell Function > Necroptosis

Necroptosis

Necroptosis is a form of programmed cell death that occurs in response to various cellular stressors, such as infection, inflammation, or injury.
This process is characterized by the activation of receptor-interacting protein kinase 1 (RIPK1) and receptor-interacting protein kinase 3 (RIPK3), leading to the formation of a necrosome complex and the eventual lysis of the cell.
Necroptosis is distinct from apoptosis, as it involves the rupture of the cell membrane and the release of cellular contents, which can trigger an inflammatory response.
Understanding the mechanisms underlying necroptosis is crucial for the development of therapeutic interventions targeting this form of cell death, which has been implicated in a variety of diseases, including neurodegeneration, ischemia-reperfusion injury, and certain types of cancer.
PubCompare.ai can help researchers optimize their necroptosis research by identifying the best protocols and products to streamline their efforts and drive meaningful results.

Most cited protocols related to «Necroptosis»

1
Necroptosis-Associated lncRNA Identification
The necroptosis gene set M24779.gmt contains eight necroptosis genes, and it was downloaded from the Gene Set Enrichment Analysis (GSEA) (http://www.gsea-msigdb.org/gsea/index.jsp). In addition, with previous reports about necroptosis, we finally obtained the profile of 67 necroptosis-related genes (Appendix T1). Then we found 5,022 differentially expressed lncRNAs (Log2 fold change (FC) > 1, false discovery rate (FDR) < 0.05, and p < 0.05) after screening the synthetic data matrix by Strawberry Perl and limma R package [12 (link)]. Correlation analysis was performed between 67 necroptosis-related genes and differentially expressed lncRNAs in the combined matrices. Then, 387 lncRNAs, with necroptosis-related genes, Pearson correlation coefficients >0.4, and p < 0.001, were considered necroptosis-related lncRNAs.
Zhao Z., Liu H., Zhou X., Fang D., Ou X., Ye J., Peng J, & Xu J. (2021). Necroptosis-Related lncRNAs: Predicting Prognosis and the Distinction between the Cold and Hot Tumors in Gastric Cancer. Journal of Oncology, 2021, 6718443.
Full text: Click here
Publication 2021
Genes Genetic Profile Necroptosis RNA, Long Untranslated Strawberries
2
Genetic Manipulation of Cell Death Pathways
CRISPR-mediated knockout plasmids containing guide RNAs targeting BAX, BAK1, NCKAP1, ACSL4, SLC7A11, CYFIP1, WAVE-2, Abi2, HSPC300 were generated in lentiCRISPR v2 (Addgene, #52961) according to the standard protocol. The SLC7A11 cDNA–containing expression construct was described in previous publications25 , 26 . The lentiviral construct expressing membrane-bound green fluorescent protein (mGFP) (#22479) and Rac1-Q61L cDNA-containing construct (#84605) were obtained from Addgene. NCKAP1 cDNA and shRNA constructs targeting RPN1, N-WASP, WHAMM were obtained from the Functional Genomics Core Facility of The University of Texas MD Anderson Cancer Center. NCKAP1 and Rac1-Q61L cDNA were subsequently cloned into the vector pLX302 with a C-terminal V5 tag (Addgene, #25896). WAVE-2 constructs were provided by Dr. Daniel D. Billadeau. All constructs were confirmed by DNA sequencing. The sequences of gRNAs and shRNA used in this study are listed in Supplementary Table 4. Necroptosis inhibitor Nec-1s (#2263) was from BioVision, and necrosis inhibitor Necrox-2 (#ALX-430-166-M001) was from Enzo. Ferroptosis inducer (1S,3R)-RSL3 (#19288) and apoptosis inducer staurosporine (#81590) were from Cayman Chemical. L-[1, 2, 1', 2'-14C]-cystine (#NEC854010UC) was from PerkinElmer. KL-11743 was from Kadmon. The following reagents were obtained from Sigma-Aldrich: 2-deoxy-D-glucose (#D8375-1G), Trolox (#238813), 4-Hydroxy-TEMPO (Tempol) (#176141), beta-mercaptoethanol (2ME) (#M6250), deferoxamine mesylate salt (DFO) (#D9533), ferrostatin-1 (#SML0583), chloroquine (#C6628), diamide (#D3648), diethyl-maleate (#D97703, BAY-876 (#SML1774), and L-Cystine (#C7602). All reagents were dissolved according to manufacturers’ instructions.
Atkin W.S., Hart A., Edwards R., Cook C.F., Wardle J., McIntyre P., Aubrey R., Baron C., Sutton S., Cuzick J., Senapati A, & Northover J.M. (2000). Single blind, randomised trial of efficacy and acceptability of oral Picolax versus self administered phosphate enema in bowel preparation for flexible sigmoidoscopy screening. BMJ : British Medical Journal, 320(7248), 1504-1509.
Publication 2023
2-Mercaptoethanol ABI2 protein, human Apoptosis BAK1 protein, human BAY-876 Caimans Chloroquine Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats Cystine Diamide diethyl maleate DNA, Complementary Ferroptosis ferrostatin-1 Glucose Malignant Neoplasms Membrane Proteins Mesylate, Deferoxamine NCKAP1 protein, human Necroptosis Necrosis oxytocin, 1-desamino-(O-Et-Tyr)(2)- Plasmids RNA Salts Short Hairpin RNA Staurosporine tempol TEMPOL-H Trolox C WASL protein, human
3
Prognostic Modeling of Necroptosis-Related LncRNAs
According to the clinical data of GC cases in the TCGA and GTEx, univariate Cox proportional hazard regression analysis was used to screen lncRNAs related to survival from necroptosis-related lncRNA (p < 0.05). Then, we made the Lasso regression performed with 10-fold cross-validation and a p value of 0.05 as well as run for 1,000 cycles. For each cycle, a random stimulation was set up 1,000 times in order to prevent overfitting. Then a model was established. The 1-, 2-, and 3-year time-dependent receiver operating characteristics (ROC) curves of the model were plotted by the calculation procedure. We calculated the risk score with the following formula: risk score=∑k=1ncoeflncRNAk∗exprlncRNAk, where the coef (lncRNAn) was the short form of the coefficient of lncRNAs correlated with survival and expr (lncRNAn) was the expression of lncRNAs. According to the median risk score, subgroups including low- and high-risk groups were established [12 (link), 13 (link)]. We used the chi-square test to analyze the relationship between the model and clinical factors in order to evaluate the prognostic value of the constructed model.
Zhao Z., Liu H., Zhou X., Fang D., Ou X., Ye J., Peng J, & Xu J. (2021). Necroptosis-Related lncRNAs: Predicting Prognosis and the Distinction between the Cold and Hot Tumors in Gastric Cancer. Journal of Oncology, 2021, 6718443.
Full text: Click here
Publication 2021
Necroptosis Population at Risk RNA, Long Untranslated
4
Apoptosis Induction and Gene Expression
Each cell type was divided into three groups, H2O2 treatment, positive control (DTT treatment), and negative control. H2O2 groups were incubated in 0.1, 0.2, 0.4, 0.8, or 1.6 mM H2O2, the positive control groups were incubated in 2 mM DTT, and the negative control groups received no treatment. After the start of exposure, cells were examined every 0.5 h by staining with AO/EB to monitor the initiation of apoptosis. DAPI staining was used to determine the time to substantial apoptosis. Then, apoptosis times and cell survival rates were determined by AO/EB staining and DAPI staining. Finally, total RNA was extracted from cells using Trizol reagent (Invitrogen, Carlsbad, CA, USA) to assess expression levels of apoptosis- and necroptosis-associated genes. Reverse transcription was performed using a PrimeScript II 1st Strand cDNA synthesis kit (TaKaRa, Otsu, Shiga, Japan). Quantitative real-time PCR was conducted to evaluate changes in caspase-9, P53, NF-κB, and RIP expression levels using the primers and thermocycle conditions shown in Table 1. Group means were compared by ANOVA using SPSS17.0 software. All bar figures were created by Graphpad Prism 5 software.
Xiang J., Wan C., Guo R, & Guo D. (2016). Is Hydrogen Peroxide a Suitable Apoptosis Inducer for All Cell Types?. BioMed Research International, 2016, 7343965.
Full text: Click here
Publication 2016
Anabolism Apoptosis Caspase 9 Cells DAPI DNA, Complementary Genes Necroptosis neuro-oncological ventral antigen 2, human Oligonucleotide Primers Peroxide, Hydrogen prisma Real-Time Polymerase Chain Reaction RELA protein, human Reverse Transcription trizol
5
Bibliometric Analysis of Necroptosis Research
The Web of Science Core Collection (WoSCC) database is widely used in bibliometrics, which contains Science Citation Index Expanded (SCIE), Social Science Citation Index (SSCI), and Emerging Sources Citation Index (ESCI) (21 (link), 24 (link)). Data were obtained from the WoSCC database on March 24, 2022. The search formula was [TS = (“necroptosis” OR “necroptotic”)] AND [Publication type = (Article)] AND [Language = (English)], and the publication year was limited to (2012-2021). Search results were downloaded as “Full Record and Cited References” and “Plain Text”. For further analysis, we subsequently renamed the files as “download_*.txt”, which CiteSpace software could read.
Zhang J., Song L., Jia J., Tian W., Lai R., Zhang Z., Li J., Ju J, & Xu H. (2022). Knowledge Mapping of Necroptosis From 2012 to 2021: A Bibliometric Analysis. Frontiers in Immunology, 13, 917155.
Full text: Click here
Publication 2022
Necroptosis

Most recents protocols related to «Necroptosis»

1
Quantifying Necroptosis-Induced LDH Release
LDH release in cell supernatant were measured using LDH activity kit (Beyotime, C0017) according to the manufacturer’s protocol. Relative changes in the release of LDH were presented as fold ± SEM of individual control groups without treatment of necroptosis inducer TSZ and GDCA.
Yang S., Chang N., Li W., Yang T., Xue R., Liu J., Zhang L., Yao X., Chen Y., Wang H., Yang L., Huang J, & Li L. (2023). Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis. Cell Death & Disease, 14(3), 175.
Full text: Click here
Publication 2023
Cells Necroptosis
2
Bile Acid Mediated Necroptosis Induction
Sodium taurocholate hydrate (TCA, 86339), glycocholic acid hydrate (GCA, G2878), and sodium glycodeoxycholate (GDCA, G9910) were obtained from Sigma-Aldrich (St. Louis, MO, USA). JTE-013 (10009458) was obtained from Cayman Chemical (Ann Arbor, MI, USA). Necroptosis inducer kit TSZ (C1058S, TNFα plus Smac mimetic and a pan-caspase inhibitor z-VAD-FMK) was obtained from Beyotime (Shanghai, China). Necrosulfonamide (NSA, HY-100573) was obtained from MCE (NJ, USA).
Yang S., Chang N., Li W., Yang T., Xue R., Liu J., Zhang L., Yao X., Chen Y., Wang H., Yang L., Huang J, & Li L. (2023). Necroptosis of macrophage is a key pathological feature in biliary atresia via GDCA/S1PR2/ZBP1/p-MLKL axis. Cell Death & Disease, 14(3), 175.
Full text: Click here
Publication 2023
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone Caimans Caspase Inhibitors Glycine Deoxycholate Glycocholic Acid JTE 013 N-(4-(N-(3-methoxypyrazin-2-yl)sulfamoyl)phenyl)-3-(5-nitrothiophene-2-yl)acrylamide Necroptosis Sodium Sodium Hydroxide Taurocholate Tumor Necrosis Factor-alpha
3
Investigating Cell Death Interactions
For better knowing the interactions between our CRLncSig and other types of “cell death”, we adopted the Pearson analysis. Apoptosis, necroptosis, and pyroptosis-related genes were extracted from the GeneCard and Gene Set Enrichment Analysis (GSEA) online databases, respectively, by applying the following steps: 1) search the GeneCard using the corresponding keyword; 2) search the GSEA using the corresponding keyword: 3) merge the above results and take the unique genes. FerrDb is the first database dedicated to ferroptosis regulators and ferroptosis-related diseases (Zhou and Bao, 2020 (link)). Ferroptosis-related genes were obtained from FerrDb (http://www.datjar.com:40013/bt2104/).
Ma C., Li F., He Z., Zhao S., Yang Y, & Gu Z. (2023). Prognosis and personalized treatment prediction in lung adenocarcinoma: An in silico and in vitro strategy adopting cuproptosis related lncRNA towards precision oncology. Frontiers in Pharmacology, 14, 1113808.
Full text: Click here
Publication 2023
Apoptosis Cells Ferroptosis Genes Necroptosis Pyroptosis
4
Identifying Necroptosis Correlates in GBM
WGCNA was carried out to identify the gene modules mostly correlated with necroptosis score in GBM.
Zheng P., Ren D., Cong Y., Xiaoxue Z.h.a.n.g, & Zhang Y. (2023). Single-Cell Sequencing Reveals Necroptosis-Related Prognostic Genes of Glioblastoma. Oxidative Medicine and Cellular Longevity, 2023, 2926655.
Full text: Click here
Publication 2023
Gene Modules Necroptosis
5
Necroptosis Gene Landscape Exploration
571 genes related to necroptosis were obtained from the GeneCards website. And a total of 68 necroptosis-related genes were found with a correlation efficiency > 1.
Zheng P., Ren D., Cong Y., Xiaoxue Z.h.a.n.g, & Zhang Y. (2023). Single-Cell Sequencing Reveals Necroptosis-Related Prognostic Genes of Glioblastoma. Oxidative Medicine and Cellular Longevity, 2023, 2926655.
Full text: Click here
Publication 2023
Genes Genes, vif Necroptosis

Top products related to «Necroptosis»

1
Nec 1 by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, United Kingdom
Nec-1 is a laboratory instrument designed for the analysis and quantification of cellular apoptosis. It is an established tool for researchers studying programmed cell death mechanisms and their implications in various biological processes and disease states.
2
Necrostatin 1 by Merck Group
Sourced in United States, Germany, United Kingdom, China, Sao Tome and Principe, Morocco
Necrostatin-1 is a laboratory compound that functions as a selective inhibitor of necroptosis, a form of programmed cell death. It acts by blocking the receptor-interacting serine/threonine-protein kinase 1 (RIPK1), a key regulator of necroptosis. Necrostatin-1 is commonly used in scientific research to study cell death pathways.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Z vad fmk by Merck Group
Sourced in United States, Germany, China, Canada, Italy, Sao Tome and Principe, United Kingdom, Japan, Macao, Australia
Z-VAD-FMK is a caspase inhibitor that functions as a broad-spectrum inhibitor of caspase enzymes. It is commonly used in research applications to study apoptosis and cell death processes.
5
Z vad fmk by Enzo Life Sciences
Sourced in United States, Germany, France, Switzerland, United Kingdom
Z-VAD-FMK is a broad-spectrum caspase inhibitor. It binds irreversibly to the catalytic site of caspase enzymes, thereby blocking their activity.
6
Z vad fmk by Selleck Chemicals
Sourced in United States, China, Germany, Switzerland, United Kingdom
Z-VAD-FMK is a broad-spectrum caspase inhibitor. It functions by irreversibly binding to the catalytic site of caspase enzymes, thereby blocking their activity.
7
Facscalibur by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
8
Z vad fmk by R&D Systems
Sourced in United States, Germany, United Kingdom
Z-VAD-FMK is a caspase inhibitor that irreversibly binds to the catalytic site of caspase enzymes. It is commonly used in research applications to investigate the role of caspases in cellular processes.
9
Celltiter glo luminescent cell viability assay by Promega
Sourced in United States, Germany, United Kingdom, Switzerland, Italy, France, Japan, Spain, Sweden, Belgium, China, Australia
The CellTiter-Glo Luminescent Cell Viability Assay is a quantitative method for determining the number of viable cells in a cell-based assay. The assay measures the amount of ATP present, which is an indicator of metabolically active cells.
10
Cycloheximide (chx) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Japan, Spain, Canada, Switzerland, India, Israel, Brazil, Poland, Portugal, Australia, Morocco, Sweden, Austria, Senegal, Belgium
Cycloheximide is a laboratory reagent commonly used as a protein synthesis inhibitor. It functions by blocking translational elongation in eukaryotic cells, thereby inhibiting the production of new proteins. This compound is often utilized in research applications to study cellular processes and mechanisms related to protein synthesis.

More about "Necroptosis"

Necroptosis is a form of programmed cell death that occurs in response to various cellular stressors, such as infection, inflammation, or injury.
This process is characterized by the activation of receptor-interacting protein kinase 1 (RIPK1) and receptor-interacting protein kinase 3 (RIPK3), leading to the formation of a necrosome complex and the eventual lysis of the cell.
Necroptosis is distinct from apoptosis, as it involves the rupture of the cell membrane and the release of cellular contents, which can trigger an inflammatory response.
Understanding the mechanisms underlying necroptosis is crucial for the development of therapeutic interventions targeting this form of cell death, which has been implicated in a variety of diseases, including neurodegeneration, ischemia-reperfusion injury, and certain types of cancer.
Researchers can leverage the power of PubCompare.ai, an AI-driven platform, to optimize their necroptosis research by identifying the best protocols and products to streamline their efforts and drive meaningful results.
PubCompare.ai can help researchers locate and compare protocols from literature, pre-prints, and patents, utilizing advanced AI techniques to pinpoint the most effective approaches.
This includes identifying the optimal use of compounds like Nec-1 (Necrostatin-1), a potent inhibitor of necroptosis, as well as the appropriate use of cell culture media like FBS (Fetal Bovine Serum) and cell viability assays like the CellTiter-Glo Luminescent Cell Viability Assay.
By leveraging PubCompare.ai, researchers can enhance the reproducibility of their necroptosis studies and accelerate their progress towards understanding this critical cell death pathway and developing effective therapies.
Whether investigating the role of necroptosis in neurodegeneration, ischemia-reperfusion injury, or cancer, PubCompare.ai can help optimize research efforts and drive meaningful results.