Log In Sign up
The largest database of trusted experimental protocols
> Physiology > Clinical Attribute > Oxygen Consumption

Oxygen Consumption

Oxygen Consumption is the process of utilizing oxygen by an organism or tissue to support metabolic processes.
It is an important physiological parameter that provides insights into cellular respiration, energy production, and overall health.
This MeSH term describes the measurement and analysis of oxygen consumption rates, which can be used to evaluate mitochondrial function, exercise capacity, and the effects of various interventions on metabolic activity.
Researchers can leverage PubCompare.ai's AI-driven tools to efficiently locate and compare the most effective oxygen consumption protocols from scientific literature, preprints, and patents, saving time and effort in their research endeavors.
Explore PubCompare.ai's powerful platform today and take your oxygen consumption studies to the next level.

Most cited protocols related to «Oxygen Consumption»

1
Simultaneous Measurement of Physical Activity and Energy Expenditure
Study participants spent approximately 24-h period in a whole-room indirect calorimeter (28 (link)), and followed a structured protocol for simultaneous measurements of PA and EE. The protocol included a broad range of pursuits ranging from moderate and vigorous to light and sedentary tasks, including eating meals and snacks and self-care activities. During times (30 to 120 minutes) when no activity was specifically scheduled, the participants were asked to engage in their normal daily routine as much as possible without specific suggestions. They also recorded their activities in a diary with a detailed schedule, reporting any episodes of accidental monitor nonwear intervals and other relevant comments. Sleep was defined as the period of time spent lying on a mattress at night between 9:00 pm and 6:00 am without any significant movement as determined by the floor (force platform) in the room calorimeter. The participants were instructed how to record their activities in a provided diary with a detailed schedule and a timeline. They checked off each scheduled activity and reported any episodes of accidental monitor nonwear intervals and other relevant information (e.g. treadmill speed) or comments. During the day, staff was available for assistance and the dairy was discussed with each participant after finishing the study.
Body weight was measured to the nearest 0.01 kg with a digital scale and height was measured using a wall-mounted stadiometer. The minute-to-minute EE was calculated from the rates of oxygen consumption and carbon dioxide production (33 (link)). Nonwear EE was calculated by summing EE measured by the room calorimeter during time intervals detected as nonwear by each algorithm.
The PA was measured by commercially available Actigraph GT1M accelerometer (ActiGraph, Pensacola, FL), calibrated by the manufacturer placed on the anterior axillary line of the hip on the dominant side of the body. Among commercially available accelerometers, the Actigraph used in the present study provides consistent and high quality data, supported by its feasibility, reliability and validity (9 (link)). The monitor reports counts from the summation of the measured accelerations over a specified epoch (1 ). Actigraph data were collected at a 1-second epoch and summed as counts per minute.
Choi L., Liu Z., Matthews C.E, & Buchowski M.S. (2011). Validation of Accelerometer Wear and Nonwear Time Classification Algorithm. Medicine and science in sports and exercise, 43(2), 357-364.
Publication 2011
Acceleration Accidents Actigraphy Axilla Body Weight Carbon dioxide EPOCH protocol Human Body Light Movement Oxygen Consumption Sleep Snacks TimeLine
2
Comparative Mitochondrial Respiration Assays
Clark electrode assays performed for comparative purposes utilized a Hansatech Oxytherm apparatus (PP Systems, Amesbury, MA) for rat heart mitochondria or a Rank system (Rank Brothers, Bottisham, Cambridge, England) for mouse liver mitochondria. For rat heart mitochondria, assays were performed in parallel with the same mitochondrial preparation, MAS, substrates and compounds as for the XF24 assays. Typically 62.5–125 µg of mitochondria were used in a volume of 500 µl MAS plus the appropriate substrate. Respiration was initiated by adding mitochondria, and followed by sequential addition of ADP, oligomycin and FCCP. Concentrations of substrate, ADP, oligomycin, and FCCP were identical to those used in the XF24 experiments. For mouse liver mitochondria, assays were performed in parallel the same mitochondrial preparation, MAS, substrates and compounds as for the XF24 assays with the following modifications: substrate was 5 mM succinate, 2 µM rotenone and 300 µM ADP was used. Typically, 0.3 mg/ml of mitochondria were used in a volume of 2.0–3.5 ml MAS plus the appropriate substrate. Respiration was initiated by adding mitochondria, followed by sequential addition of ADP, oligomycin and FCCP. Concentrations of oligomycin and FCCP were identical to those used in the XF24 experiments. Oxygen consumption rates were converted from nmol O/min/ml to pmol O2/min/µg mitochondrial protein.
Rogers G.W., Brand M.D., Petrosyan S., Ashok D., Elorza A.A., Ferrick D.A, & Murphy A.N. (2011). High Throughput Microplate Respiratory Measurements Using Minimal Quantities Of Isolated Mitochondria. PLoS ONE, 6(7), e21746.
Publication 2011
Biological Assay Brothers Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Cell Respiration Mice, House Mitochondria Mitochondria, Heart Mitochondria, Liver Mitochondrial Proteins Oligomycins Oxygen Consumption Rotenone Succinate
3
Comparative Analysis of Respiration Measurement Methods in C. elegans
There are several methods available to measure respiration of living samples, which can be globally divided into two groups: O2-dependent quenching of porphyrin-based phosphors (Seahorse Bioscience XF respirometer and Luxcel MitoXpress) and amperometric O2 sensors (Clark electrodes, including the widely adopted Oroboros system)1 ,42 (link). Historically, the amperometric approach has been the main method used to assess mitochondrial respiration in C. elegans. For the amperometric approach, nematodes are delivered into a single respiratory chamber, which is separated from two half-cells by O2-permeable material. In this way, only O2 can diffuse from the assay medium through the membrane. When a small voltage is applied to the half-cells, O2 is reduced by electrons at the cathode yielding hydrogen peroxide. Subsequently, H2O2 oxidizes the Ag (silver) of the Ag/AgCl anode, which results in an electrical current that is proportional to the O2 pressure – and thus concentration – in the experimental respiratory chamber.
Apart from the detection modality, differences of the XF respirometric method appear at the level of number of worms per assay, replicates, multiple, or real-time measurements and the ability to inject compounds during an experiment (Table 1). The Clark electrode approach requires thousands (~2000-5000) of worms in a single chamber to obtain an estimation of the oxygen consumption rate43 (link). Performing multiple measurements, biological replicates and comparing conditions provide the biggest challenges within the Clark electrode method as the traditional set-up only allows the measurements of one sample at a time. In contrast, a XF96 respirometer requires ~10-20 worms per well to acquire a reproducible oxygen consumption rate, measurements can be easily and quickly (in the order of minutes) repeated in an automated way and since XF respirometers can analyse whole plates at the same time, about 96 conditions/replicates can be tested at once. An additional difference is the presence of drug-injection ports that can be programmed to inject compounds in all 96 wells at time points that are specified a priori during an XF respirometer experiment. Clark electrode systems also allow injection of compounds, and even offer flexibility with respect to the timing, dosing and number of additions as compounds are injected manually during the course of the assay. However, precise timing of manual additions between replicate experiments may be challenging.
More similar to the Seahorse XF respirometer method is the Luxcel MitoXpress O2 consumption assay, which relies on O2-dependent quenching of porphyrin-based phosphor. The MitoXpress kits provide a way of performing real-time analysis of cellular respiration, via an oxygen-quenching fluorophore system. Worms are placed into the wells of a 96- or 384-well plate, the kit reagents are added, and measurements are made in a fluorometric plate reader. Multiple conditions and replicates can be tested side-by-side in the wells of a single plate, but repeated measurements over time are more challenging as there is typically no automatized mixing system integrated in the plates or plate-readers to restore basal O2 levels. In addition, single estimation of the OCR takes >90 minutes, while careful estimations of the OCR in the XF respirometer approach takes only 2-5 minutes of measuring time. Finally, the use of compounds to assess multiple aspects of mitochondrial function related to oxygen consumption is limited since compounds need to be injected manually immediately prior to the start of the experiment.
Koopman M., Michels H., Dancy B.M., Kamble R., Mouchiroud L., Auwerx J., Nollen E.A, & Houtkooper R.H. (2016). A screening-based platform for the assessment of cellular respiration in Caenorhabditis elegans. Nature protocols, 11(10), 1798-1816.
Publication 2016
Biological Assay Biopharmaceuticals Cell Respiration Cells DNA Replication Electricity Electrons Fluorometry Helminths Mitochondria Nematoda Oxygen Consumption Permeability Peroxide, Hydrogen Pharmaceutical Preparations Phosphorus Porphyrins Pressure Respiration Respiratory Rate Seahorses Silver Tissue, Membrane
4
Measuring Mitochondrial Function in BAEC

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2010
Cells Mitochondrial Inheritance Oxygen Oxygen Consumption Protons Seahorses Transients
5
Validating Actigraph Accelerometers against VO2
Using a similar methodology as described by Freedson et al. [13 (link)], each accelerometer was initialized per manufacturers instructions, and the sampling period was set at 1 minute, raw output was expressed as counts per minute (cpm) prior to each testing session. To evaluate the validity of the GT1M (Actigraph, Pensacola, FL) and the GT3X (Actigraph, Pensacola, FL) against oxygen consumption, all participants wore both accelerometers simultaneously, over the right hip and secured it with the same adjustable elastic belt and buckle supplied by the manufacturers. After a standardized 10 minute familiarization period on a calibrated treadmill, participants performed 6 minutes of the following exercise conditions: slow walking (4.8 km.h-1), fast walking (6.4 km.h-1), and running (9.7 km.h-1), and the order of exercise conditions was random across participants. All exercise bouts were performed at 0% grade due to the known limitations of the GT1M [15 (link)]. Each exercise bout was separated by a 5-minute rest period, this rest period was standardized for each participant, as previously described by Freedson et al. [13 (link)]. While study participants reported no know contradictions to exercise, as a precaution, exercise heart rate was monitored using a Polar T31 transmitter and receiver (Lake Success, NY, US). Following the testing session both accelerometers were immediately downloaded as per the manufactures instructions using firmware version 5.10.
Kelly L.A., McMillan D.G., Anderson A., Fippinger M., Fillerup G, & Rider J. (2013). Validity of actigraphs uniaxial and triaxial accelerometers for assessment of physical activity in adults in laboratory conditions. BMC Medical Physics, 13, 5.
Publication 2013
Actigraphy Oxygen Consumption Rate, Heart

Most recents protocols related to «Oxygen Consumption»

1
Restoring Mitochondrial Function in Friedreich's Ataxia Neurons
Not available on PMC !

Example 2

The next experiments asked whether inhibition of the same set of FXN-RFs would also upregulate transcription of the TRE-FXN gene in post-mitotic neurons, which is the cell type most relevant to FA. To derive post-mitotic FA neurons, FA(GM23404) iPSCs were stably transduced with lentiviral vectors over-expressing Neurogenin-1 and Neurogenin-2 to drive neuronal differentiation, according to published methods (Busskamp et al. 2014, Mol Syst Biol 10:760); for convenience, these cells are referred to herein as FA neurons. Neuronal differentiation was assessed and confirmed by staining with the neuronal marker TUJ1 (FIG. 2A). As expected, the FA neurons were post-mitotic as evidenced by the lack of the mitotic marker phosphorylated histone H3 (FIG. 2B). Treatment of FA neurons with an shRNA targeting any one of the 10 FXN-RFs upregulated TRE-FXN transcription (FIG. 2C) and increased frataxin (FIG. 2D) to levels comparable to that of normal neurons. Likewise, treatment of FA neurons with small molecule FXN-RF inhibitors also upregulated TRE-FXN transcription (FIG. 2E) and increased frataxin (FIG. 2F) to levels comparable to that of normal neurons.

It was next determined whether shRNA-mediated inhibition of FXN-RFs could ameliorate two of the characteristic mitochondrial defects of FA neurons: (1) increased levels of reactive oxygen species (ROS), and (2) decreased oxygen consumption. To assay for mitochondrial dysfunction, FA neurons an FXN-RF shRNA or treated with a small molecule FXN-RF inhibitor were stained with MitoSOX, (an indicator of mitochondrial superoxide levels, or ROS-generating mitochondria) followed by FACS analysis. FIG. 3A shows that FA neurons expressing an NS shRNA accumulated increased mitochondrial ROS production compared to EZH2- or HDAC5-knockdown FA neurons. FIG. 3B shows that FA neurons had increased levels of mitochondrial ROS production compared to normal neurons (Codazzi et al., (2016) Hum Mol Genet 25(22): 4847-485). Notably, inhibition of FXN-RFs in FA neurons restored mitochondrial ROS production to levels comparable to that observed in normal neurons. In the second set of experiments, mitochondrial oxygen consumption, which is related to ATP production, was measured using an Agilent Seahorse XF Analyzer (Divakaruni et al., (2014) Methods Enzymol 547:309-54). FIG. 3C shows that oxygen consumption in FA neurons was ˜60% of the level observed in normal neurons. Notably, inhibition of FXN-RFs in FA neurons restored oxygen consumption to levels comparable to that observed in normal neurons. Collectively, these preliminary results provide important proof-of-concept that inhibition of FXN-RFs can ameliorate the mitochondrial defects of FA post-mitotic neurons.

Mitochondrial dysfunction results in reduced levels of several mitochondrial Fe-S proteins, such as aconitase 2 (ACO2), iron-sulfur cluster assembly enzyme (ISCU) and NADH:ubiquinone oxidoreductase core subunit S3 (NDUFS3), and lipoic acid-containing proteins, such as pyruvate dehydrogenase (PDH) and 2-oxoglutarate dehydrogenase (OGDH), as well as elevated levels of mitochondria superoxide dismutase (SOD2) (Urrutia et al., (2014) Front Pharmacol 5:38). Immunoblot analysis is performed using methods known in the art to determine whether treatment with an FXN-RF shRNA or a small molecule FXN-RF inhibitor restores the normal levels of these mitochondrial proteins in FA neurons.

US11873494B2. Genetic and pharmacological transcriptional upregulation of the repressed FXN gene as a therapeutic strategy for Friedreich ataxia (2024-01-16). University of Massachusetts [US]. Inventors: Michael R. Green [US], Minggang Fang [US].
Patent 2024
Aconitate Hydratase Biological Assay Cells Cloning Vectors Enzymes EZH2 protein, human frataxin Genets HDAC5 protein, human Histone H3 Immunoblotting Induced Pluripotent Stem Cells inhibitors Iron Ketoglutarate Dehydrogenase Complex Mitochondria Mitochondrial Inheritance Mitochondrial Proteins MitoSOX NADH NADH Dehydrogenase Complex 1 NEUROG1 protein, human Neurons Oxidoreductase Oxygen Consumption Proteins Protein Subunits Psychological Inhibition Pyruvates Reactive Oxygen Species Repression, Psychology Seahorses Short Hairpin RNA Sulfur sulofenur Superoxide Dismutase Superoxides Thioctic Acid Transcription, Genetic
2
Cardiorespiratory Assessment in Exercise Protocol
All participants underwent a graded CPET on a bicycle ergometer (Ergoselect 150P, ergoline GmbH, Bitz, Germany) within 1 week before HIIT. Minute ventilation ( V E) and oxygen consumption ( V O2) were measured breath by breath using a computer-based system (CareFusion MasterScreen CPX, CPX International Inc., Germany). V O2peak was defined as described in the ACSM guidelines for graded CPETs [26 ]. The  oxygen uptake efficiency sloe (OUES) during exercise was determined as described in our previous work [7 (link)]. A noninvasive continuous cardiac output (CO) monitoring system (NICOM, Cheetah Medical, Wilmington, DE, USA) was used to measure peak CO (COex) during CPET. The CPET procedure and determination of cardiorespiratory parameters are detailed in Additional file 2.
Hsu C.C., Wang J.S., Shyu Y.C., Fu T.C., Juan Y.H., Yuan S.S., Wang C.H., Yeh C.H., Liao P.C., Wu H.Y, & Hsu P.H. (2023). Hypermethylation of ACADVL is involved in the high-intensity interval training-associated reduction of cardiac fibrosis in heart failure patients. Journal of Translational Medicine, 21, 187.
Publication 2023
Cardiac Output Cheetahs Clostridium perfringens epsilon-toxin Ergoline Oxygen Oxygen Consumption
3
Transient Transfection of PERK and E-Syt1 Constructs in HEK293T and HeLa Cells
HEK293T cells were transiently transfected with PERK full length-myc, PERK-K622A -myc, myc-empty vector, eGFP-empty vector, eGFP-tagged E-Syt1, eGFP-E-Syt1-ΔCDE, eGFP-E-Syt1-ΔSMP or eGFP-E-Syt1-ΔDE using Trans-IT X2 transfection reagent accordingly to the manufacturer’s instructions. HeLa cells were transiently transfected with human HRP-KDEL-myc, Mitochondrial Aequorin WT, mCherry-empty vector, mCherry-tagged E-Syt1, mCherry-E-Syt1-ΔCDE, eGFP-empty vector, eGFP-E-Syt1, eGFP-E-Syt1-ΔCDE, eGFP-Syt1-ΔSMP or eGFP-E-Syt1-ΔDE using Lipofectamin 2000 (Thermo Fisher Scientific) or electroporated with 4D-Nucleofector (Lonza Bioscience) using SE Cell Line kit (Lonza Bioscience). 24 h after transfections, cells were replated to (microscopy) culture dishes (Mattek corporation) or collected for lysate after 48 h. For Oxygen Consumption Rate (OCR) analysis, cells were plated after nucleofection, and OCR analysis was performed using a Seahorse XF24 (Agilent) Extracellular Flux Analyzer 24 h later.
Sassano M.L., van Vliet A.R., Vervoort E., Van Eygen S., Van den Haute C., Pavie B., Roels J., Swinnen J.V., Spinazzi M., Moens L., Casteels K., Meyts I., Pinton P., Marchi S., Rochin L., Giordano F., Felipe-Abrio B, & Agostinis P. (2023). PERK recruits E-Syt1 at ER–mitochondria contacts for mitochondrial lipid transport and respiration. The Journal of Cell Biology, 222(3), e202206008.
Publication 2023
Aequorin Cell Lines Cells Cloning Vectors HeLa Cells Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Microscopy Mitochondrial Inheritance Oxygen Consumption Seahorses SYT1 protein, human Transfection
4
Extracellular Flux Analysis of HK-2 Cells
Oxygen consumption rate (OCR) was measured using the SeahorseXF96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA, United States). HK-2 cells were seeded into 96-well cell culture plate at a density of 1.5 × 104 cells. When the cell confluence was about 90%, cells were washed twice with assay medium (49.5 mL basal medium, 500 μL sodium pyruvate and basal medium) and incubated in a non-CO2 incubator for 40–60 min, OCR was measured. The working fluid concentration was as follows: oligomycin (1 μM), FCCP (2.5 μM), rotenone and antimycin A (1 μM).
Dai Q., Zhang H., Tang S., Wu X., Wang J., Yi B., Liu J., Li Z., Liao Q., Li A., Liu Y, & Zhang W. (2023). Vitamin D-VDR (vitamin D receptor) alleviates glucose metabolism reprogramming in lipopolysaccharide-induced acute kidney injury. Frontiers in Physiology, 14, 1083643.
Publication 2023
Antimycin A Biological Assay Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Cell Culture Techniques Cells Oligomycins Oxygen Consumption Pyruvate Rotenone Seahorses Sodium
5
Metabolic Profiling of B Cells and A20 Cells
Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were measured using an XFe24 Extracellular Flux Analyzer (Seahorse Bioscience). A20 cells (0.8 × 106 per well) and primary B lymphocytes (2 × 106 per well) were resuspended in XF DMEM pH 7.4 media supplemented with 1 mM pyruvate, 2 mM glutamine, and 10 mM glucose (Mito Stress Test) and plated in poly-L-lysine coated microchamber wells. For the Mito Stress Test, 1.5 μM oligomycin, 2 μM FCCP, and 0.5 μM antimycin/rotenone were utilized. Data were analyzed using the Agilent Seahorse Wave Software and normalized to total protein context per well using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific).
Emrich S.M., Yoast R.E., Zhang X., Fike A.J., Wang Y.H., Bricker K.N., Tao A.Y., Xin P., Walter V., Johnson M.T., Pathak T., Straub A.C., Feske S., Rahman Z.S, & Trebak M. (2023). Orai3 and Orai1 mediate CRAC channel function and metabolic reprogramming in B cells. eLife, 12, e84708.
Publication 2023
antimycin B-Lymphocytes Biological Assay Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Cells Exercise Tests Glucose Glutamine Gold Lysine Mitomycin Oligomycins Oxygen Consumption Poly A Proteins Pyruvate Rotenone Seahorses

Top products related to «Oxygen Consumption»

1
Xf24 extracellular flux analyzer by Agilent Technologies
Sourced in United States, France
The XF24 Extracellular Flux Analyzer is a lab equipment product from Agilent Technologies. It is designed to measure the oxygen consumption rate and extracellular acidification rate of cells in real-time.
2
Xf96 extracellular flux analyzer by Agilent Technologies
Sourced in United States
The XF96 Extracellular Flux Analyzer is a laboratory instrument designed to measure the metabolic activity of cells in a high-throughput manner. The device is capable of simultaneously assessing the oxygen consumption rate and extracellular acidification rate of cells, providing insights into their respiratory and glycolytic activity.
3
Oxygraph 2k by Oroboros
Sourced in Austria
The Oxygraph-2k is a high-performance respirometer designed for precise measurement of oxygen consumption and production in biological samples. It provides real-time monitoring of oxygen levels, making it a valuable tool for researchers in the fields of cell biology, physiology, and bioenergetics.
4
Oligomycin by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Sao Tome and Principe, Japan, Macao, France, China, Belgium, Canada, Austria
Oligomycin is a laboratory product manufactured by Merck Group. It functions as an inhibitor of the mitochondrial F1F0-ATP synthase enzyme complex, which is responsible for the synthesis of adenosine triphosphate (ATP) in cells. Oligomycin is commonly used in research applications to study cellular bioenergetics and mitochondrial function.
5
Seahorse xfe96 analyzer by Agilent Technologies
Sourced in United States, Germany
The Seahorse XFe96 Analyzer is a high-throughput instrument designed for real-time measurement of cellular metabolism. The analyzer uses microplates to assess oxygen consumption rate and extracellular acidification rate, providing insights into cellular bioenergetics.
6
Antimycin a by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, Sao Tome and Principe, China, Japan, Macao, France, Belgium
Antimycin A is a chemical compound that acts as a potent inhibitor of mitochondrial respiration. It functions by blocking the electron transport chain, specifically by interfering with the activity of the cytochrome bc1 complex. This disruption in the respiratory process leads to the inhibition of cellular respiration and energy production within cells.
7
Rotenone by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, Sao Tome and Principe, China, Macao, Spain, India, Japan, France, Belgium, Israel, Canada
Rotenone is a naturally occurring insecticide and piscicide derived from the roots of certain tropical plants. It is commonly used as a research tool in laboratory settings to study cellular processes and mitochondrial function. Rotenone acts by inhibiting the electron transport chain in mitochondria, leading to the disruption of cellular respiration and energy production.
8
Seahorse xf cell mito stress test kit by Agilent Technologies
Sourced in United States, Germany, Canada
The Seahorse XF Cell Mito Stress Test Kit is a laboratory equipment product designed to measure mitochondrial function in live cells. It provides real-time analysis of key parameters such as oxygen consumption rate and extracellular acidification rate, which are indicators of cellular respiration and metabolic activity.
9
Xf cell mito stress test kit by Agilent Technologies
Sourced in United States, United Kingdom
The XF Cell Mito Stress Test Kit is a laboratory equipment product from Agilent Technologies designed to measure mitochondrial function in live cells. It provides real-time analysis of key parameters related to cellular respiration and energy production.
10
Xf24 analyzer by Agilent Technologies
Sourced in United States
The XF24 Analyzer is a laboratory instrument manufactured by Agilent Technologies. It is designed to measure the oxygen consumption rate and extracellular acidification rate of cells in a multi-well plate format. The XF24 Analyzer provides researchers with real-time, non-invasive data on cellular metabolic activity.

More about "Oxygen Consumption"

Oxygen uptake, cellular respiration, metabolic rate, energy production, mitochondrial function, exercise capacity, metabolic activity, aerobic metabolism, oxidative phosphorylation, VO2 measurement, respiratory quotient, RQ, XF24 Extracellular Flux Analyzer, XF96 Extracellular Flux Analyzer, Oxygraph-2k, Oligomycin, Seahorse XFe96 Analyzer, Antimycin A, Rotenone, Seahorse XF Cell Mito Stress Test Kit, XF Cell Mito Stress Test Kit, XF24 Analyzer.
Oxygen consumption is a critical physiological parameter that provides insights into an organism's or tissue's metabolic processes.
It reflects the utilization of oxygen to support cellular respiration and energy production.
Measuring and analyzing oxygen consumption rates can be used to evaluate mitochondrial function, exercise capacity, and the effects of various interventions on metabolic activity.
Researchers can leverage advanced tools like the Seahorse XF Analyzers and Oxygraph-2k to accurately quantify oxygen consumption and gain valuable insights into cellular bioenergetics.
By utilizing these technologies and comparing the effectiveness of different oxygen consumption protocols, researchers can optimize their studies and uncover new discoveries in the field of metabolism and cellular function.
PubCompare.ai's AI-driven platform can help researchers efficiently locate and compare the most effective oxygen consumption protocols from scientific literature, preprints, and patents, saving time and effort in their research endeavors.
Explore PubCompare.ai's powerful tools today and take your oxygen consumption studies to the next level.