A quadrapole ICP-MS with a Micromist nebulizer and a Scott Double Pass spray chamber (Agilent 7500 CE, Agilent Technologies, CA, USA) was used for single particle analysis of the nanoparticle samples. The data acquisition for the instrument was set to time-resolved analysis (TRA) mode, thus collecting intensities as a function of time (i.e. counts/dwell-time interval). The measurement duration of each run was 30 s with a data acquisition rate, or dwell time, of 10 ms/event. At the beginning of each run the instrument was tuned using a multi-element tune solution for optimal sensitivity and minimum oxide and double-charged species levels (Table S-1, Supporting Information ). The tune solution was made in-house using 1 µg/L Li, Co. Y, Tl, Ce, and Ba in 1% v/v hydrochloric acid (Merck, Darmstadt, Germany). A calibration curve was produced using dissolved standards (AccuTrace, CT, USA) prepared in 0.2% trace pure nitric acid (Merck, Darmstadt, Germany). The peristaltic pump was set to 0.05 rps for all experiments, which translates to a sample flow rate of approximately 0.18 mL/min. However, given the potential for slight day to day differences, the flow rate was measured during each experiment. Due to the rapid data sampling rate, only one isotope (107Ag for silver and 197Au for gold) was monitored during analysis. Data, in the form of counts per dwell-time interval as a function of time, were exported to a spreadsheet for further processing.
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Organ or Tissue Function
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Peristalsis
Peristalsis
Peristalsis refers to the rhythmic, wave-like muscular contractions that propel contents through the digestive tract.
This complex physiological process involves coordinated movements of the esophagus, stomach, small intestine, and large intestine.
Peristalsis ensures the efficient transport of food, liquids, and waste products, supporting vital digestive functions.
Understanding the mechanism and regulation of peristalsis is crucial for investigating gastrointestinal disorders, developing treatments, and optimizing research protocols.
Leveraging AI-driven comparisons can help researchers identify the most accurate and reproducible peristalsis protocols from literature, preprints, and patents, streamlining the research process and achiving high-quality results.
This complex physiological process involves coordinated movements of the esophagus, stomach, small intestine, and large intestine.
Peristalsis ensures the efficient transport of food, liquids, and waste products, supporting vital digestive functions.
Understanding the mechanism and regulation of peristalsis is crucial for investigating gastrointestinal disorders, developing treatments, and optimizing research protocols.
Leveraging AI-driven comparisons can help researchers identify the most accurate and reproducible peristalsis protocols from literature, preprints, and patents, streamlining the research process and achiving high-quality results.
Most cited protocols related to «Peristalsis»
Gold
Hydrochloric acid
Hypersensitivity
Isotopes
Nebulizers
Nitric acid
Oxides
Peristalsis
Silver
Single Molecule Analysis
The intravital imaging preparation used in this study is similar to previously described methods11 (link),15 (link) with the following differences: imaging is performed with the tissue within the peritoneal cavity, fecal material is not scraped from the mucosal surface and in some experiments atropine (1 mg/kg) was injected subcutaneously to dampen peristaltic movement of the small intestine. At this dose atropine, did not affect the formation of TEDs or GAPs. Model fluorescent antigens, dextran (2–5mg), ovalbumin (2mg), BSA, (2mg) and FluoSpheres (1ml undiluted) (all from Invtirogen, Carlsbad, CA) were injected into the intestinal lumen ~2hrs minutes prior to imaging. Human resection specimens were incubated in 10ug/ml of dextran at room temperature for 1hr prior to imaging.
Antigens
Atropine
Defecation
Dextran
Feces
Homo sapiens
Intestines
Mucous Membrane
Ovalbumin
Peristalsis
Peritoneal Cavity
Tissues
Total RNA (0.4–16 mg for E. coli) was dissolved in 2 ml binding buffer [1.2 M NaCl, 30 mM HEPES-KOH (pH 7.5), 15 mM EDTA and 0.5 mM DTT]. Affinity tip-columns were attached to the pipettor-head, and pipetted 40 times in the RNA mixture incubated in sample reservoir at 66°C. Aspiration volume for each tip-column was 200 μl. After each pipetting cycle, the RNA solution was moved by a peristaltic pump into a mixing reservoir and then pumped back into the sample reservoir and incubated for 1 min to maintain the desired temperature. Twelve 400 μl washing buffer [0.1 M NaCl, 2.5 mM HEPES-KOH (pH 7.5), 1.25 mM EDTA and 0.5 mM DTT] and six 400 μl elution buffer [20 mM NaCl, 0.5 mM HEPES-KOH (pH 7.5), 0.25 mM EDTA and 0.5 mM DTT] tubes were prepared for each tip-column, and the tip-columns were pipetted three times in each tube at 40°C for washing and 68°C for elution. The eluted RNAs were precipitated with 2-propanol. The total yield from each tip-column was calculated by measuring UV absorbance at 260 nm.
Buffers
Edetic Acid
Escherichia coli
Head
HEPES
Peristalsis
Propanols
RNA
Sodium Chloride
Acclimatization
Buffers
Cardiac Arrest
Chromatography
enzyme activity
Enzymes
Glutathione
Peristalsis
polyvalent mechanical bacterial lysate
Protease Inhibitors
Proteins
Proteolysis
Reducing Agents
Resins, Plant
SDS-PAGE
Sepharose
Sepharose 4B
Serine Proteinase Inhibitors
Staphylococcal Protein A
Antibodies
Aorta
Brain
Cannula
Cranium
Dyes
Heart
Human Body
Left Ventricles
Needles
Nitrogen
Perfusion
Peristalsis
Poly(ADP-ribose) Polymerases
Polymerization
Rodent
Spinal Cord
Subdural Space
Most recents protocols related to «Peristalsis»
A 20 MHz 33220A function/arbitrary waveform generator (Agilent Technologies Inc., Santa Clara, CA, USA) was used to drive the coils. Amplification of this input signal was performed using a 1000 W RF power amplifier, model 1140LA (Electronics & Innovation Ltd., Rochester, NY, USA). The amplified signal runs through the L-matching circuit that enables maximum power transfer from the source to load (coil). Since the coil dissipates heat due to resistive losses, a Masterflex® peristaltic pump circulates water through the instrument’s body to remove excess heat. A simple air-cooled heat exchanger configuration maintains the circulating water at a constant temperature of about 24 °C to avoid the temperature increasing throughout the duration of the experiment. Temperatures of the test samples (e.g. cells, MNPs) and surroundings were monitored with a FOTEMP-PLUS signal conditioner using TS3–10 mm-06 fiber optic temperature sensors (Optocon AG, Dresden, Germany) and stored in a computer using the FOTEMP-ASSISTANT 2.3 configuration and data logging software, also from Optocon AG. The temperature of the samples was measured by passing the fiber optic thermal sensor through a lid with a hole and the surrounding temperature was measured by placing another fiber optic sensor close to the plate. The reusable lid was cleaned with ethanol after each run to avoid contamination. Subsection E in Supplementary Information shows top-view and side-view of the samples placed on the devices (see figure S9 and figure S10), as well as an example of the temperatures reached during MFH (see figures S11 and S12).
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Cells
Ethanol
Heat Loss
Medical Devices
Peristalsis
Water, Body
Expression and purification were performed as above, using the strain E. coli BL21 carrying either carrying pGEX4T3_stop or pGEX4T3_YgfB. Differing from above, the expression was carried out at 25 °C. For resuspension and lysis of the bacterial pellet, GST-A buffer (50 mM Tris, 150 mM NaCl, 1 mM DTT, pH 7.5) supplemented with lysozyme, Triton X-100, DNase and protease inhibitor was used. For purification, a GSTrap™ HP 1 ml column (Cytiva) connected to a peristaltic pump was used. After loading the column and collecting the flow through the column was washed using GST-A buffer and the protein eluted using GST-B-buffer (50 mM Tris, 150 mM NaCl, 10 mM reduced glutathione, pH 8). After column regeneration, the flow through was loaded on the column once again and also washed and eluted. The obtained eluate fractions were pooled and dialysed against 10 liter of PBS pH 7.4 and 0.5 mM DTT using a ZelluTrans (Roth) dialysis tube with a 3.4 kDa cutoff and frozen in dialysis buffer. Analysis by SDS-PAGE and protein storage was done as described above.
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Bacteria
Buffers
Deoxyribonucleases
Dialysis
Escherichia coli
Freezing
Muramidase
Peristalsis
Protease Inhibitors
Proteins
Reduced Glutathione
Regeneration
SDS-PAGE
Sodium Chloride
Strains
Triton X-100
Tromethamine
To determine the fouling behavior
of fabricated ceramic membranes, the same cross-flow filtration test
mechanism was used as previously described in earlier sections. To
prepare three different fouling test solutions, 1 mg mL–1 of BSA as a fouling agent was dissolved in 100 mL PBS. Acidities
of test solutions were set to different pH values by dropwise addition
of 0.25 M HCl and 0.25 M NaOH solutions. To ensure complete fouling
during tests, acidity of the first solution was set to pH 5.5, and
to ensure complete antifouling during tests the basicity of the second
solution was set to pH 9.5. For the determination of fouling behavior
at physiological alkalinity, the third solution was balanced at pH
7.5 with the aforementioned approach. Before the fouling tests, the
solution feed rate was calibrated to 50 mL min–1 on the peristaltic pump. After the solution was initialized into
the system and the first permeate was observed, the permeating solution
was weighed with a high-precision scale for 1 min for each timepoint.
The measurements were recorded every 0, 15, 30, 60, 90, and 120 min
after the first permeate. After the tests had been continued overnight,
samples were collected every 16 and 24 h after the first permeate.
of fabricated ceramic membranes, the same cross-flow filtration test
mechanism was used as previously described in earlier sections. To
prepare three different fouling test solutions, 1 mg mL–1 of BSA as a fouling agent was dissolved in 100 mL PBS. Acidities
of test solutions were set to different pH values by dropwise addition
of 0.25 M HCl and 0.25 M NaOH solutions. To ensure complete fouling
during tests, acidity of the first solution was set to pH 5.5, and
to ensure complete antifouling during tests the basicity of the second
solution was set to pH 9.5. For the determination of fouling behavior
at physiological alkalinity, the third solution was balanced at pH
7.5 with the aforementioned approach. Before the fouling tests, the
solution feed rate was calibrated to 50 mL min–1 on the peristaltic pump. After the solution was initialized into
the system and the first permeate was observed, the permeating solution
was weighed with a high-precision scale for 1 min for each timepoint.
The measurements were recorded every 0, 15, 30, 60, 90, and 120 min
after the first permeate. After the tests had been continued overnight,
samples were collected every 16 and 24 h after the first permeate.
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Alkalies
Filtration
Heartburn
Peristalsis
physiology
Tissue, Membrane
Carrier preparation: Half of the coke
from the Shaanxi region was used as the carrier, and the other half
was crushed to a 200 mesh and set aside. SC powder (5 g) was taken,
and remove it with HF (20 mL, 10%). It was then filtered and washed
until neutral. Subsequently, it was dried at 80 °C for 2 h, impregnated
with aqueous KOH solution (45 mL, 5 mol/L) for 2 h, filtered, washed
until neutral, and finally dried at 80 °C for 2 h. After drying,
the sample was placed in a quartz boat, and N2 was passed
through the tube furnace. The sample was then activated at 800 °C
for 2 h with a heating rate of 5 °C/min and then heated, which
resulted in the formation of the modified active SC (ASC).
Catalyst preparation:
The metal ion
nitrate was weighed proportionally, an appropriate amount of deionized
water was added, and the contents were stirred thoroughly. Next, an
excess of anhydrous Na2CO3 was weighed and dissolved
in deionized water. A certain amount of deionized water was heated
to 75 °C and stirred; when the water temperature became stable
at 75 °C, the metal nitrate and Na2CO3 were
added dropwise into the beaker using a peristaltic pump, and the flow
of Na2CO3 was adjusted to maintain the pH in
the range of 7–8. At the end of dropwise addition, the pH was
maintained in the range of 7–8. After the dropwise addition,
precipitate aging was carried out at room temperature, and it was
washed until no NO3– was left behind
and dried at 80 °C, and ground ASC was added. Next, HNO3 solution (1 mol/L) was continuously added during the grinding process,
and after sufficient grinding, the sample was dried at 80 °C
and taken out. Finally, it was roasted at 450 °C for 4 h in a
muffle furnace, and FeMnCe/ASC was obtained after roasting.
Metals
NCOA6 protein, human
Nitrates
Peristalsis
Powder
Quartz
Mycobacterium bovis BCG strain was cultured in a 2 liter bioreactor under growth conditions (Table
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Amino Acids
Bioreactors
Calmette-Guerin Bacillus
Carbon
Glycerin
Growth Disorders
Isotopes
Nitrogen
Peristalsis
Strains
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The Minipuls 3 is a peristaltic pump designed for a variety of laboratory applications. It features a compact and robust construction, delivering precise and consistent flow rates across a wide range of flow ranges. The Minipuls 3 is suitable for a variety of tasks, including liquid transfer, dosing, and sample preparation.
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A peristaltic pump is a type of positive displacement pump that uses the principle of peristalsis to move fluid. It operates by the sequential contraction and relaxation of the pump's tubing, which creates a moving zone of occlusion that forces the fluid to move through the tubing in a specific direction.
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The peristaltic pump is a type of positive displacement pump used to transport fluids by the progressive compression of a flexible tube, known as the 'tubing' or 'hose,' placed in a circular pump casing or raceway. The movement of the pump rotor compresses the tubing, forcing the fluid to move through the tube.
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Masterflex is a versatile peristaltic pump system designed for various laboratory applications. It features a durable construction and precise flow control, enabling consistent and reliable fluid handling.
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The Mini Spray Dryer B-290 is a laboratory equipment designed for spray drying applications. It uses a two-fluid nozzle to atomize the feed material into fine droplets, which are then dried in a stream of hot air. The Mini Spray Dryer B-290 is a compact and versatile unit suitable for a wide range of applications, including the production of powders, granules, and microencapsulated particles.
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More about "Peristalsis"
Peristalsis is a critical physiological process that involves the rhythmic, wave-like contractions of the digestive tract muscles.
This complex mechanism ensures the efficient transport of food, liquids, and waste products through the esophagus, stomach, small intestine, and large intestine.
Understanding the regulation and dynamics of peristalsis is crucial for investigating gastrointestinal disorders, developing effective treatments, and optimizing research protocols.
Researchers can leverage cutting-edge technologies like Minipuls 3, Buscopan, and Masterflex peristaltic pumps to study and manipulate peristaltic movements.
These advanced tools, along with the Masterflex L/S and Mini Spray Dryer B-290, can help researchers simulate and analyze the complex patterns of peristalsis, providing valuable insights into the underlying mechanisms.
By utilizing AI-driven comparisons, researchers can identify the most accurate and reproducible peristalsis protocols from literature, preprints, and patents.
This streamlined approach, facilitated by platforms like PubCompare.ai, can help optimize research workflows and achieve high-quality results.
Additionally, the Minipuls 2 and Ingenia systems offer further capabilities for studying and understanding the nuances of peristaltic movements.
Overall, the understanding and control of peristalsis is crucial for advancements in gastrointestinal health, and researchers can leverage a diverse array of technologies and techniques to explore this vital physiological process.
This complex mechanism ensures the efficient transport of food, liquids, and waste products through the esophagus, stomach, small intestine, and large intestine.
Understanding the regulation and dynamics of peristalsis is crucial for investigating gastrointestinal disorders, developing effective treatments, and optimizing research protocols.
Researchers can leverage cutting-edge technologies like Minipuls 3, Buscopan, and Masterflex peristaltic pumps to study and manipulate peristaltic movements.
These advanced tools, along with the Masterflex L/S and Mini Spray Dryer B-290, can help researchers simulate and analyze the complex patterns of peristalsis, providing valuable insights into the underlying mechanisms.
By utilizing AI-driven comparisons, researchers can identify the most accurate and reproducible peristalsis protocols from literature, preprints, and patents.
This streamlined approach, facilitated by platforms like PubCompare.ai, can help optimize research workflows and achieve high-quality results.
Additionally, the Minipuls 2 and Ingenia systems offer further capabilities for studying and understanding the nuances of peristaltic movements.
Overall, the understanding and control of peristalsis is crucial for advancements in gastrointestinal health, and researchers can leverage a diverse array of technologies and techniques to explore this vital physiological process.