Study participants spent approximately 24-h period in a whole-room indirect calorimeter (28 (link)), and followed a structured protocol for simultaneous measurements of PA and EE. The protocol included a broad range of pursuits ranging from moderate and vigorous to light and sedentary tasks, including eating meals and snacks and self-care activities. During times (30 to 120 minutes) when no activity was specifically scheduled, the participants were asked to engage in their normal daily routine as much as possible without specific suggestions. They also recorded their activities in a diary with a detailed schedule, reporting any episodes of accidental monitor nonwear intervals and other relevant comments. Sleep was defined as the period of time spent lying on a mattress at night between 9:00 pm and 6:00 am without any significant movement as determined by the floor (force platform) in the room calorimeter. The participants were instructed how to record their activities in a provided diary with a detailed schedule and a timeline. They checked off each scheduled activity and reported any episodes of accidental monitor nonwear intervals and other relevant information (e.g. treadmill speed) or comments. During the day, staff was available for assistance and the dairy was discussed with each participant after finishing the study.
Body weight was measured to the nearest 0.01 kg with a digital scale and height was measured using a wall-mounted stadiometer. The minute-to-minute EE was calculated from the rates of oxygen consumption and carbon dioxide production (33 (link)). Nonwear EE was calculated by summing EE measured by the room calorimeter during time intervals detected as nonwear by each algorithm.
The PA was measured by commercially available Actigraph GT1M accelerometer (ActiGraph, Pensacola, FL), calibrated by the manufacturer placed on the anterior axillary line of the hip on the dominant side of the body. Among commercially available accelerometers, the Actigraph used in the present study provides consistent and high quality data, supported by its feasibility, reliability and validity (9 (link)). The monitor reports counts from the summation of the measured accelerations over a specified epoch (1 ). Actigraph data were collected at a 1-second epoch and summed as counts per minute.
Body weight was measured to the nearest 0.01 kg with a digital scale and height was measured using a wall-mounted stadiometer. The minute-to-minute EE was calculated from the rates of oxygen consumption and carbon dioxide production (33 (link)). Nonwear EE was calculated by summing EE measured by the room calorimeter during time intervals detected as nonwear by each algorithm.
The PA was measured by commercially available Actigraph GT1M accelerometer (ActiGraph, Pensacola, FL), calibrated by the manufacturer placed on the anterior axillary line of the hip on the dominant side of the body. Among commercially available accelerometers, the Actigraph used in the present study provides consistent and high quality data, supported by its feasibility, reliability and validity (9 (link)). The monitor reports counts from the summation of the measured accelerations over a specified epoch (1 ). Actigraph data were collected at a 1-second epoch and summed as counts per minute.