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Determination of Death

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Most cited protocols related to «Determination of Death»

1
Detailed Liver Injury and Fibrosis Protocols
Alcoholic and hepatitis B-associated cirrhotic liver samples (stage 3-4 fibrosis) were collected from donor livers during liver transplantation from the Liver Tissue Cell Distribution System (LTCDS), University of Minnesota. The LTCDs were supported by NIH Contract #N01-DK-7-0004 / HHSN267200700004C. Additional information on the sample preparation, age and gender of the donors is provided in the Supplemental Materials.
The details of the induction of liver injury and fibrosis by CCl4 and bile duct ligation (BDL) and the treatment protocols are described in the Supplemental Methods.
The determination of liver function, histology and immunohistochemistry, quantitative analysis of hepatic fibrosis are described in the Supplemental Methods.
The determination of hepatic PARP and myeloperoxidase activities, 4-hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), and hydroxyproline contents, real-time PCR, Western immunoblot analysis are described in the Supplemental Methods.
Other procedures such as isolation and treatments of murine hepatic hepatocytes and stellate cells, cell death determination by flow cytometer and activation of hepatic stellate cells are also described in the Supplemental Methods.
Mukhopadhyay P., Rajesh M., Cao Z., Horváth B., Park O., Wang H., Erdelyi K., Holovac E., Wang Y., Liaudet L., Hamdaoui N., Lafdil F., Haskó G., Szabo C., Boulares A.H., Gao B, & Pacher P. (2014). Poly (ADP-ribose) polymerase-1 is a key mediator of liver inflammation and fibrosis. Hepatology (Baltimore, Md.), 59(5), 1998-2009.
Publication 2013
3-nitrotyrosine 4-hydroxy-2-nonenal Alcoholics CCL4 protein, human Cell Death Cells Cell Transplantation Determination of Death Donors Duct, Bile Fibrosis Fibrosis, Liver Hepatic Stellate Cells Hepatitis B Hepatocyte Hydroxyproline Immunohistochemistry Injuries isolation Ligation Liver Mus Peroxidase Portal System Real-Time Polymerase Chain Reaction Tissue Donors Tissues Tissue Transplantation Transplantation Treatment Protocols Western Blot
2
Validating Verbal Autopsy Diagnoses
This validation study was conducted on all 219 deaths reported in GCS participants by the end of January 2005. Copies of all 219 completed VA questionnaires were given to two trained internists, henceforth referred to as the “reviewers”, who were different individuals from the internists who made the first GCS cause of death determinations. The reviewers studied the completed VA questionnaires independently, and made their decisions on the cause of death based on the VA questionnaire alone, without having any other medical documents. In order to get an estimate of within-reviewer reliability of the VA diagnoses, the same two reviewers were asked to review the VA's a second time one month later, without the knowledge that this was a repeat review.
For the purpose of this study the causes of death were categorized into one of nine major categories. To estimate the reliability, kappa statistics were calculated for the agreement between the reviewers' diagnoses. Both within-reviewer reliability (comparing the first and second diagnoses of the same reviewer) and between-reviewer reliability (comparing the diagnoses made by the two reviewers) were calculated. Multi-rater agreement was calculated and its confidence interval was calculated using bootstrap technique. To estimate validity, the VA diagnoses made by the two reviewers were compared to the gold standard diagnoses and the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and kappa statistics were calculated for each reviewer-gold standard comparison. As a sensitivity analysis, the VA validity was once calculated in the subgroup of 149 cases (68%) who had both VA and medical documents available for the gold standard cause of death determinations, and then in all 219 cases.
All study participants had signed written informed consent at enrollment phase of GCS and ethical approval for the present study was obtained from the ethics committee of Digestive Disease Research Center of Tehran University of Medical Sciences.
Khademi H., Etemadi A., Kamangar F., Nouraie M., Shakeri R., Abaie B., Pourshams A., Bagheri M., Hooshyar A., Islami F., Abnet C.C., Pharoah P., Brennan P., Boffetta P., Dawsey S.M, & Malekzadeh R. (2010). Verbal Autopsy: Reliability and Validity Estimates for Causes of Death in the Golestan Cohort Study in Iran. PLoS ONE, 5(6), e11183.
Publication 2010
Determination of Death Diagnosis Digestive System Disorders Ethics Committees, Research Gold Hypersensitivity
3
Psychological Autopsy of Suicide Victims
This study was conducted during June 2014 to September 2015. Interviewers from the three research sites were trained intensively for 10 days on the determination of manner of death, method of psychological autopsy, interview skills, and administration of study instruments.
Interviews with informants of suicide victims were scheduled 2–6 months after death, while interviews with informants of living comparisons were conducted as soon as the participants and their informants were identified. Two informants for each suicide victim and living comparison were identified: generally, the first informant was one next-to-kin who lived with the suicide victim or living comparison, and the second informant was always a friend, a neighbor, or a remote relative. Each informant was interviewed separately by one trained interviewer. The average interview time was 90 min.
The study was approved by the IRB of the Central South University, Shandong University, and Guangxi Medical University. The aim and procedure of the research were explained to all participants. Written informed consent must be obtained before interviews were conducted.
Zhou L., Wang G., Jia C, & Ma Z. (2018). Being left-behind, mental disorder, and elderly suicide in rural China: a case–control psychological autopsy study. Psychological Medicine, 49(3), 458-464.
Publication 2018
Autopsy Determination of Death Friend Interviewers Psychological Techniques
4
Annexin V-FITC/PI Apoptosis Assay
Apoptotic cells were measured by staining with FITC-conjugated Annexin V/propidium iodide (PI) (BD Pharmingen, San Diego, CA, USA) and determining with flow cytometer according to the manufacturer’s instructions. Both early apoptotic (Annexin V-positive, PI-negative) and late apoptotic (Annexin V-positive and PI-positive) cells were included in cell death determinations.
Guo Y., Zhu X, & Sun X. (2020). COTI-2 induces cell apoptosis in pediatric acute lymphoblastic leukemia via upregulation of miR-203. Bioengineered, 11(1), 201-208.
Publication 2020
Annexin A5 Apoptosis Cells Determination of Death Fluorescein-5-isothiocyanate Propidium Iodide
5
Utah Suicide Genetic Study
In collaboration with the centralized, statewide Utah Office of Medical Examiner (OME), the authors obtained DNA samples from ~6000 persons who died by suicide. The centralized OME and conservative determination helped to maximize the accuracy of suicide case status.21 Suicide cause-of-death determination results from a detailed investigation of the scene of the death and circumstances of death, determination of medical conditions by full autopsy, review of medical and other public records concerning the case, interviews with survivors, and standard toxicology workups. Suicide determination is traditionally made quite conservatively due to its impact on surviving relatives.
DNA from suicide deaths were extracted from whole blood using the Qiagen Autopure LS automated DNA extractor (www.qiagen.com). Genotyping is described below. Data collection and genotyping for the Utah Suicide Study is ongoing. To date, 4,381 samples have been genotyped in two waves as determined by the date of sample receipt and the availability of genotyping funds (for detailed information on sample collection see Supplementary Methods S1. After quality control procedures and ancestry analysis, data are comprised of 3,413 Utah suicide deaths. The Utah population is primarily Northwestern European in ancestry, a relatively genetically homogeneous group with low inbreeding across generations, comparable to the rest of the United States.22 (link)
Docherty A.R., Shabalin A.A., DiBlasi E., Monson E., Mullins N., Adkins D.E., Bacanu S.A., Bakian A.V., Crowell S., Darlington T.M., Callor B., Christensen E.D., Gray D., Keeshin B., Klein M., Anderson J.S., Jerominski L., Hayward C., Porteous D.J., McIntosh A., Li Q, & Coon H. (2020). Genome-wide association study of suicide death and polygenic prediction of clinical antecedents. The American journal of psychiatry, 177(10), 917-927.
Publication 2020
Autopsy BLOOD Determination of Death Europeans Specimen Collection Survivors

Most recents protocols related to «Determination of Death»

1
Orthotopic Liver Transplantation Protocols
An orthotopic transplantation of a standard criteria donated (SCD) whole organ of a deceased donor after brain death (DBD) after static cold storage (SCS) was defined as standard OLT. The recipient hepatectomy was performed by retrohepatic caval resection without venovenous bypass and the biliary anastomosis by duct-to-duct reconstruction. Deviations from this standard OLT were recorded.
From February 2018, normothermic machine perfusion (NMP) was implemented on a routine basis for donations after circulatory determination of death (DCD) in the case of surgically highly complex recipients or high-risk patients or for logistic reasons in the case of limited operative resources) [54 (link)].
Extended criteria were defined according to the Eurotransplant Foundation rules (donor age >65 years, donor body mass index >30 kg/m2, ICU stay with ventilation >7 days, serum sodium >165 mmol/L, hepatic steatosis >40%, total bilirubin >3 mg/dL, aspartate aminotransferase (AST) >90 U/L, alanine aminotransferase (ALT) >105 U/land DCD) [55 (link)].
Breitkopf R., Treml B., Senoner T., Bukumirić Z, & Rajsic S. (2023). Invasive Fungal Breakthrough Infections under Targeted Echinocandin Prophylaxis in High-Risk Liver Transplant Recipients. Journal of Fungi, 9(2), 272.
Publication 2023
Aspartate Transaminase Bilirubin Blood Circulation Brain Death Cryopreservation D-Alanine Transaminase Determination of Death Donor, Organ Donors Duct, Bile Fatty Liver Hepatectomy Index, Body Mass Operative Surgical Procedures Patients Perfusion Reconstructive Surgical Procedures Serum Sodium Surgical Anastomoses Transplantation Venae Cavae
2
Liver Transplantation Protocols: Standard and Extended Criteria
A standard OLT was defined as deceased donor transplantation of a standard criteria donor (SCD) whole organ after static cold storage (SCS) from a donation after brain death (DBD). The recipient hepatectomy was performed by retrohepatic caval resection without a veno-venous bypass, and the biliary anastomosis by duct-to-duct reconstruction. Deviations from this technique (e.g., split liver donation, extended criteria donation (ECD), donation after circulatory determination of death (DCD), the use of a veno-venous bypass, an inferior vena cava preservation by “piggyback” technique, or a Roux-en-y choledochojejunostomy) were recorded.
Extended criteria donors were defined according to the Eurotransplant Foundation rules by the following criteria: donor age >65 years, ICU stay with ventilation > 7 days, donor BMI > 30 kg/m2, hepatic steatosis > 40%, serum sodium > 165 mmol/L, alanine aminotransferase (ALT) > 105 U/L, aspartate aminotransferase (AST) > 90 U/L, total bilirubin > 3 mg/dL, and DCD [42 (link)].
Normothermic machine perfusion (NMP) was introduced at our center in February 2018 and has meanwhile been implemented in a daily routine for the following indications [43 (link)]:

Donor-related: in cases of ECD, especially if prolonged ischemia times are expected

Recipient-related: in cases of surgically highly complex recipients or high-risk patients

Logistic-related: in case of limited resources (e.g., parallel organ transplantations or overlap with other urgent interventions).

Breitkopf R., Treml B., Simmet K., Bukumirić Z., Fodor M., Senoner T, & Rajsic S. (2023). Incidence of Invasive Fungal Infections in Liver Transplant Recipients under Targeted Echinocandin Prophylaxis. Journal of Clinical Medicine, 12(4), 1520.
Publication 2023
Aspartate Transaminase Bilirubin Biologic Preservation Brain Death Cardiovascular System Choledochojejunostomy Cryopreservation D-Alanine Transaminase Determination of Death Donor, Organ Donors Duct, Bile Fatty Liver Hepatectomy Ischemia Liver Operative Surgical Procedures Organ Transplantation Patients Perfusion Reconstructive Surgical Procedures Roux-en-Y Anastomosis Serum Sodium Surgical Anastomoses Transplantation Veins Vena Cavas, Inferior Venae Cavae
3
Out-of-Hospital Cardiac Arrest Patient Data Analysis
Patient data was retrospectively collected from patients with EMS care report. The form consisted of EMS operation unit data, patient data, and all EMS team’s treatments recorded by the dispatcher and operating EMS staff. The following data was extracted and saved in a Microsoft excel program: (1) OHCA patient characteristics, including age (years), gender, underlying disease, etiology, location, witnessed arrest, bystander CPR performed, bystander AED applied; (2) prehospital management by EMS, including initial arrest rhythm, defibrillation, advanced airway management, prehospital fluid management, medication use during CPR process, ROSC at scene, determination of death at scene; and (3) EMS processing time, including response time (minutes), on-scene time (minutes), transportation time (minutes), and hospital arrival time (minutes).
For the operational definitions, ROSC at the scene was specified as the ability of the heart to pump blood throughout the body, a palpable pulse, and a measurable blood pressure at scene; response time was defined as the duration from emergency call to ambulance arrival at the scene; on-scene time was the duration from ambulance arrival at the scene to ambulance departure from the scene; transportation time was the duration from ambulance departure from the scene to ambulance arrival at the designated hospital; and hospital arrival time was defined as the duration from emergency call to ambulance arrival at the designated hospital.
Huabbangyang T., Klaiangthong R., Silakoon A., Sretimongkol S., Sangpakdee S., Khiaolueang M., Seancha P., Nuansamlee T., Kamsom A, & Chaisorn R. (2023). The comparison of emergency medical service responses to and outcomes of out-of-hospital cardiac arrest before and during the COVID-19 pandemic in Thailand: a cross-sectional study. International Journal of Emergency Medicine, 16, 9.
Publication 2023
Airway Management Ambulances BLOOD Blood Pressure Determination of Death Electric Countershock Emergencies Gender Heart Human Body Patients Pharmaceutical Preparations Pulse Rate
4
Observational Study on WLST Process
We performed a post hoc secondary analysis of data collected as part of a large, multi-national, prospective, observational study on the process of WLST in dying patients (the Death Prediction and Physiology after Removal of Therapy, or DePPaRT study), which collected baseline data and ECG, pulse oximeter and arterial waveforms from WLST until 30 min after determination of death. Patients in participating intensive care units in Canada, the Czech Republic, and the Netherlands were enrolled between May 2014 and December 2018. The DePPaRT study protocol, including secondary data analyses, were approved by the relevant institutional review board or ethics committee at each site (see Supplementary Table S8), and all patients’ surrogate decision makers provided written prospective informed consent for participation in the study. The protocol is compliant with the Declaration of Helsinki and data storage and analyses were performed in accordance with national legislation and General Data Protection Regulation of the European Union.
Waldauf P., Scales N., Shahin J., Schmidt M., van Beinum A., Hornby L., Shemie S.D., Hogue M., Wind T.J., van Mook W., Dhanani S, & Duska F. (2023). Machine learning determination of motivators of terminal extubation during the transition to end-of-life care in intensive care unit. Scientific Reports, 13, 2632.
Publication 2023
Arteries Critical Care Determination of Death Ethics Committees Ethics Committees, Research Patients physiology Pulse Rate Therapeutics
5
Airway Secured WLST Patients
A subgroup of DePPaRT study patients (age ≥ 18 years who died after WLST in an intensive care unit) who had their lower airway secured with an orotracheal or tracheostomy cannula were included into the analysis. As per the DePPaRT study protocol, patients were excluded if they had a neurological determination of death, a functional cardiac pacemaker, or no arterial catheter at the time of WLST. Patients who died in the ICU due to unsuccessful cardiopulmonary resuscitation were also excluded from the study.
Waldauf P., Scales N., Shahin J., Schmidt M., van Beinum A., Hornby L., Shemie S.D., Hogue M., Wind T.J., van Mook W., Dhanani S, & Duska F. (2023). Machine learning determination of motivators of terminal extubation during the transition to end-of-life care in intensive care unit. Scientific Reports, 13, 2632.
Publication 2023
Arteries Cannula Cardiopulmonary Resuscitation Catheters Determination of Death Pacemaker, Artificial Cardiac Patients Tracheostomy

Top products related to «Determination of Death»

1
Facscalibur by BD
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The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
2
Cellquest software by BD
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CellQuest software is a data acquisition and analysis software designed for flow cytometry applications. It provides tools for acquiring, processing, and analyzing flow cytometry data.
3
Annexin 5 fitc by BD
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Annexin V-FITC is a fluorescent dye-labeled protein that binds to phosphatidylserine, a cellular component exposed on the surface of apoptotic cells. It can be used to detect and quantify apoptosis in various cell types.
4
Powerwave ht by Agilent Technologies
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The PowerWave HT is a laboratory equipment product manufactured by Agilent Technologies. It is designed to provide high-throughput microwave-assisted sample preparation and digestion for various analytical techniques. The device offers precise temperature and power control to facilitate efficient sample processing.
5
Fitc conjugated annexinv propidium iodide pi by BD
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FITC-conjugated AnnexinV/propidium iodide (PI) is a laboratory reagent used for the detection and quantification of apoptosis (programmed cell death) and necrosis (accidental cell death) in cell samples. The reagent consists of a fluorescein isothiocyanate (FITC)-labeled AnnexinV protein, which binds to phosphatidylserine exposed on the surface of apoptotic cells, and the DNA-binding dye propidium iodide (PI), which can enter and stain the nuclei of necrotic cells.
6
Annexin 5 apc by BD
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Annexin V-APC is a fluorescently-labeled protein that binds to phosphatidylserine, a biomarker expressed on the surface of cells undergoing apoptosis. It is commonly used in flow cytometry applications to detect and quantify apoptotic cells.
7
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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9
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The In situ cell death determination kit is a laboratory equipment used for the detection and analysis of cell death in biological samples. The kit provides a reliable and standardized method for the identification and quantification of apoptotic or necrotic cells within a tissue or cell culture. The kit's core function is to enable researchers to assess the extent of cell death in their experimental models, supporting their investigations in various fields of study.
10
Fitc annexin 5 apoptosis detection kit 1 by BD
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The FITC Annexin V Apoptosis Detection Kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes the binding properties of the protein Annexin V, which has a high affinity for the phospholipid phosphatidylserine, which becomes exposed on the cell surface during apoptosis. The Annexin V is conjugated with the fluorescent dye FITC, allowing for the detection of apoptotic cells through fluorescence microscopy or flow cytometry.

More about "Determination of Death"

Determination of Death: Unlocking the Mysteries of Mortality The process of Determination of Death is a critical field of research, shedding light on the complex mechanisms that govern the transition from life to the great beyond.
This multifaceted topic encompasses a range of subtopics, including: - Cell death and apoptosis: Exploring the cellular pathways that lead to programmed cell death, utilizing tools like FACSCalibur, CellQuest software, Annexin V-FITC, and FITC Annexin V Apoptosis Detection Kit I. - Necrosis and tissue damage: Understanding the processes of uncontrolled cell death and its impact on the body, as examined through techniques like PowerWave HT and FITC-conjugated AnnexinV/propidium iodide (PI). - Organ failure and system collapse: Investigating the systemic failure of vital organs and the eventual cessation of life, with insights from FACScan cytofluorometer and In situ cell death determination kit. - Legal and ethical considerations: Navigating the complex landscape of end-of-life decision-making, with implications for organ donation, euthanasia, and advanced directives.
By leveraging cutting-edge AI-powered tools like PubCompare.ai, researchers can enhance the reproducibility and accuracy of their work in the field of Determination of Death.
The platform's innovative protocols and advanced AI comparisons empower scientists to effortlessly locate relevant literature, pre-prints, and patents, while identifying the most effective protocols and products to improve the quality and reliability of their research.
Whether you're a medical professional, a researcher, or simply intrigued by the mysteries of mortality, the field of Determination of Death offers a rich tapestry of insights, cutting-edge technologies, and thought-provoking ethical considerations.
Embark on a journey of discovery and unravel the secrets that lie at the heart of life and death.