The output beam was expanded (25x) to match the input window of a PALM-SLM (PPM X8267, Hamamatsu) which operates in reflection mode. A 4f telescope (L1 = 750mm, L2 = 300mm) was used to image via a dichroic mirror (DM) (Chroma Technology 425DCXR) the plane of the SLM to the rear aperture of the objective (LUMPLFL60XW/IR). The main source of power loss with an SLM device is related to the portion of un-diffracted light that forms an unwanted central spot (zero order) in the excitation field and to the light diffracted into higher orders (about 40% in total). The diffracted beam was spatially displaced from the zero order spot by introducing a phase grating in the hologram. The zero order spot, the higher orders and the ghost image were eliminated by placing a beam blocker and a diaphragm in the intermediate Fourier plane. This reduced the excitation field to a square of approximately 50×50µm2 (link). Alexa 594 was excited with a 75-W Xenon arc source coupled to a monochromator (Optoscan, Cairn Research) (centre-wavelength at 540nm, slit width 30nm) and imaged using an emission filter Chroma Technology HQ 600/40M. The laser intensity and duration were controlled using a 1MHz Digital-to-Analog Converter (DAC) (National Instruments 6713), whose output clock was linked to the clock of the Analog-to-Digital Converter (ADC), in order to synchronize the illumination pulse and voltage-clamp acquisition. Sample fluorescence was captured on a CCD camera (CoolSNAP HQ2, Roper Scientific), at the upper port of the microscope. For the experiments on cerebellum slices a similar setup was integrated into a modified photolysis system (Prairie Technologies, WI, USA) on an upright microscope (Nikon) equipped with a 100x water immersion objective (Nikon, Plan 1.1 NA) and a 405 nm diode laser (Deep Star, Omicron, Germany). For a description of preparation of brain slices, recording conditions and data analysis see
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Holography
Holography
Holography is a technique that uses interference patterns of light waves to create three-dimensional images of objects.
It involves recording the wavefronts of light reflected from an object and then reconstructing the image by illuminating the recorded pattern with a coherent light source.
Holography has a wide range of applications in fields such as imaging, data storage, and optical communication.
Researchers can use PubCompare.ai to easily locate relevant protocols from literature, pre-prints, and patents, and identify the best methods and products for their holography research needs, ensuring seamless and efficient experimentation on this important techology.
It involves recording the wavefronts of light reflected from an object and then reconstructing the image by illuminating the recorded pattern with a coherent light source.
Holography has a wide range of applications in fields such as imaging, data storage, and optical communication.
Researchers can use PubCompare.ai to easily locate relevant protocols from literature, pre-prints, and patents, and identify the best methods and products for their holography research needs, ensuring seamless and efficient experimentation on this important techology.
Most cited protocols related to «Holography»
Alexa594
Arecaceae
Brain
Cerebellum
Fluorescence
Holography
Lasers, Semiconductor
Light
Medical Devices
Microscopy
Microscopy, Fluorescence
Photolysis
Pulse Rate
Red Cell Ghost
Reflex
Submersion
Telescopes
Vaginal Diaphragm
Xenon
Eye
Fluorescence
Holography
Microscopy
Phototoxicity
Erythrocytes
Holography
Interferometry
Leukocytes
Microscopy
Neoplasm Metastasis
Reconstructive Surgical Procedures
Response, Immune
Twins
Bones
Fluorescence
Holography
Light
Microscopy
Neoplasm Metastasis
Silicon
The optical system, schematically depicted in Fig. 1 and Supplementary Fig. 11 , was built around a commercial Olympus IX71 inverted microscope, modified in order to accommodate two opposite-facing objectives, OBJ1 and OBJ2, for excitation and fluorescence collection, respectively. To this end, the condenser lens of the microscope was substituted with a dielectric mirror and an Olympus LUMPLFL60xW/IR2, NA 0.90 objective (OBJ1). The expanded (× 10) beam of a Ti:Sapphire laser (MaiTai Deep-See, Spectra-Physics) covered the active area of a first LCOS-SLM (X10468-07, Hamamatsu Photonics; SLM1), which modulated the phase of the incoming beam to create a first image of the desired intensity pattern on the diffraction grating (830 l/mm, 53004ZD02-035R, Richardson Gratings; G) for TF through the lens L1 (f1=500 mm). 2D-phase holograms were calculated using a standard GS algorithm20 (link)33 . The first diffraction order was subsequently collimated by lens L2 (f2=500 mm) and impinged on a second SLM (X10468-07, Hamamatsu Photonics; SLM2), which was imaged at the back focal plane of the excitation objective, OBJ1, via a 2:1 telescope (lenses L3, f3=1,000 mm and L4, f4=500 mm). Suppression of the zero-order spot arising from SLM1 was achieved by using two cylindrical lenses (fL1=1,000 mm and fL2=−1,000 mm) oriented at +45° and −45° with respect to the grating lines64 (link).
Holographic light patterns generated at the sample volume illuminated a thin spin-coated fluorescent layer of rhodamine-6G in polymethyl methacrylate 2% w/v in chloroform and the induced fluorescence was imaged on a CCD camera (CoolSNAP HQ2, Roper Scientific) through OBJ2 (Olympus UPLSAPO60XW, NA 1.2). For 3D reconstruction of illumination volumes, OBJ2 was fixed and focused on the fluorescent layer, while OBJ1 was moved along the axial direction with a piezo positioner of 1 mm range when working in closed loop (PI N-725.2A PIFOC). The two SLMs, the CCD camera, the piezo positioner, lasers and other electronic components of the set-up were controlled by a custom-developed interface in LabVIEW. GS-based algorithms were run in MATLAB.
When the set-up was used for generation of multiplane holographic patterns (not temporally focused) a mirror replaced the diffraction grating, and SLM2 was either used in reflectance mode by applying only the flatness correction phase mask of the device or was replaced by a mirror. Both the grating and SLM2 were mounted on magnetic bases enabling fast switching between the different configurations. The multiplane GS algorithm used in this case was run in a custom-designed C++ software interface, Wavefront Designer20 (link).
Holographic light patterns generated at the sample volume illuminated a thin spin-coated fluorescent layer of rhodamine-6G in polymethyl methacrylate 2% w/v in chloroform and the induced fluorescence was imaged on a CCD camera (CoolSNAP HQ2, Roper Scientific) through OBJ2 (Olympus UPLSAPO60XW, NA 1.2). For 3D reconstruction of illumination volumes, OBJ2 was fixed and focused on the fluorescent layer, while OBJ1 was moved along the axial direction with a piezo positioner of 1 mm range when working in closed loop (PI N-725.2A PIFOC). The two SLMs, the CCD camera, the piezo positioner, lasers and other electronic components of the set-up were controlled by a custom-developed interface in LabVIEW. GS-based algorithms were run in MATLAB.
When the set-up was used for generation of multiplane holographic patterns (not temporally focused) a mirror replaced the diffraction grating, and SLM2 was either used in reflectance mode by applying only the flatness correction phase mask of the device or was replaced by a mirror. Both the grating and SLM2 were mounted on magnetic bases enabling fast switching between the different configurations. The multiplane GS algorithm used in this case was run in a custom-designed C++ software interface, Wavefront Designer20 (link).
Chloroform
Fluorescence
Holography
Lens, Crystalline
Light
Medical Devices
Microscopy
Polymethyl Methacrylate
Reconstructive Surgical Procedures
rhodamine 6G
Sapphire
Sirolimus
Telescopes
Most recents protocols related to «Holography»
We acquired Raman spectra from spores using our custom-built LTRS instrument. The instrument is built around an inverted microscope (IX71, Olympus) [44 , 60 (link)]. We used a Gaussian laser beam operating at 785 nm (Cobolt 08-NLD) that is coupled into the microscope using a dichroic shortpass mirror with a cut-off wavelength of 650 nm (DMSP650, Thorlabs). Imaging and focusing of the beam were achieved by a 60 water immersion objective (UPlanSApo60xWIR, Olympus) with a numerical aperture of 1.2 and a working distance of 0.28 mm. The same laser was used for Raman light excitation. In general, we operated the laser at a fixed output power of 100 mW corresponding to a power of about 60 mW in the sample (total energy of 1.2 J when exposed for 20 seconds). This power chosen was well below those previously recorded to damage spores [61 (link), 62 (link)].
We collected the backscattered light by the microscope objective and passed it through a notch filter (NF785-33, Thorlabs) to reduce the Rayleigh scattered laser line. Further, to increase the signal-to-noise ratio, we mounted a 150 m diameter pinhole in the focal point of the telescope. The filtered light was coupled into our spectrometer (Model 207, McPherson) through a 150 m wide entrance slit where a 600 grooves/mm holographic grating disperses the light [63 (link)]. The Raman spectrum was then captured using a Peltier cooled CCD detector (Newton 920N-BR-DDXW-RECR, Andor) operated at -95 C. Our system has a Raman wavenumber spectral resolution of < 3 cm and accuracy of 3 cm .
We collected the backscattered light by the microscope objective and passed it through a notch filter (NF785-33, Thorlabs) to reduce the Rayleigh scattered laser line. Further, to increase the signal-to-noise ratio, we mounted a 150 m diameter pinhole in the focal point of the telescope. The filtered light was coupled into our spectrometer (Model 207, McPherson) through a 150 m wide entrance slit where a 600 grooves/mm holographic grating disperses the light [63 (link)]. The Raman spectrum was then captured using a Peltier cooled CCD detector (Newton 920N-BR-DDXW-RECR, Andor) operated at -95 C. Our system has a Raman wavenumber spectral resolution of < 3 cm and accuracy of 3 cm .
Holography
Light
Light Microscopy
Long Terminal Repeat
Microscopy
Neoplasm Metastasis
Spores
Submersion
Telescopes
The holographic recording system was the same as that used in our previous study [19 (link)], as shown in Fig. S2. The soluble corrosion products changed their concentration (Δc) at the interface during anodic dissolution, followed by a corresponding change in refractive index (Δn) and phase difference (ΔΦ). ΔΦ, Δc, and Δn are related by Equation (1) [20 (link)]. where k, λ0, and d are the concentrative refractivity, light wavelength, and geometrical path length, respectively.
Corrosion
Holography
Light
Ocular Refraction
A custom-made experimental system shown in Fig. 4 was developed with the aim of illuminating the retina with wide-angle distribution light in a coaxial way to the observation of a luminous sinusoidal stimulus. The system includes a circular glare source composed of a holographic light shaping diffuser (Light Shaping Diffusers, Luminit, LLC) back-illuminated by a white LED (Cree LED XLamp XM-L2). The luminous intensity of the source is controlled by an external dimmer. The angular distribution of the source is modified by means of removable 3D-printed masks with different angular apertures. This configuration creates uniform discs instead of the conventional annuli configurations7 (link). Throughout the manuscript, we refer to angular size as the angular distribution of the uniform discs. For this experiment, four masks were used (with diameters 12.6 mm, 17.73 mm, 21.81 mm, and 26.67 mm), so the angular sizes subtended by the glare source once it was throughout the system were 24°, 33°, 40°, and 48°.![]()
A relay telescopic system consisting of two achromatic doublet lenses conjugates the aperture of CL (45 mm focal length) with the pupil plane of the subject providing extended Maxwellian illumination. Then, the glare source and the CS test are directly projected on the retina minimizing unwanted factors affecting the disability glare measurement such as the pupil size and scattering contributions from iris and sclera27.
The visual stimuli consist of a contrast sensitivity test generated by Contrast-Test software (Opto-software). The software generates sinusoidal gratings that, through the system, present a total angular size of 9.8° with spatial frequencies of 0.2, 0.3, 0.6, 0.9, 1.2, and 1.8 cycles per degree (c/°) and contrast levels of 20, 11.1, 5.5, 3.12, 1.66, 0.87, 0.47, 0.25 and 0.12%. The luminance values of the test range from 46.5 cd/m2 for gratings with minimum contrast to 20.9 cd/m2 for grating with maximum contrast, so photopic conditions are maintained independently of the glare source luminance. A 50:50 beam splitter allows coaxial pathway of the visual target (displayed on an LCD monitor) and the glare source. The whole optical system was mounted on a 30 × 45 cm optical board coupled to a chin rest to facilitate alignment of the system axis with the subject's visual axis.
Optical layout of the experimental system: CL collimating lens, BS beam splitter.
The visual stimuli consist of a contrast sensitivity test generated by Contrast-Test software (Opto-software). The software generates sinusoidal gratings that, through the system, present a total angular size of 9.8° with spatial frequencies of 0.2, 0.3, 0.6, 0.9, 1.2, and 1.8 cycles per degree (c/°) and contrast levels of 20, 11.1, 5.5, 3.12, 1.66, 0.87, 0.47, 0.25 and 0.12%. The luminance values of the test range from 46.5 cd/m2 for gratings with minimum contrast to 20.9 cd/m2 for grating with maximum contrast, so photopic conditions are maintained independently of the glare source luminance. A 50:50 beam splitter allows coaxial pathway of the visual target (displayed on an LCD monitor) and the glare source. The whole optical system was mounted on a 30 × 45 cm optical board coupled to a chin rest to facilitate alignment of the system axis with the subject's visual axis.
Chin
Color Vision
Contrast Sensitivity
Disabled Persons
Epistropheus
Glare
Holography
Iris
Lens, Crystalline
Light
Lighting
lumin
Pupil
Retina
Sinusoidal Beds
Telescopes
Visual Pathways
The holographic haptic display (UHEV1, Ultrahaptics Ltd.) comprised a planar array of N ultrasound emitters. Such a display focuses sound in air by applying a phase delay to each transducer signal. Constructive interference yields localized regions of high differential pressure, sufficient to impart time-varying displacements to the skin (1 (link), 2 ). The differential acoustic pressure field may be described by where x is a position within the Fresnel zone of the array, an is the amplitude of the acoustic pressure wave emitted from transducer n, k = ω/c is the wave number, f = ω/(2π) = 40 kHz is the ultrasound carrier frequency, c is the speed of sound, rn = ‖x − x n‖ is the distance from x to the location x n of transducer n, and ϕn is the corresponding phase delay. Focusing at a location x f is achieved by matching the phase delays, ϕn, to the propagation time for a wavefront to reach the focus ϕn = ω‖x f − x n‖/c, thus yielding constructive interference. The focal width, δ, satisfies a Rayleigh diffraction limit, with δ ⪆ c/(2f).
Ultrasound frequency oscillations cannot be directly felt via touch. In haptic holography, palpable low-frequency mechanical signals are produced via a nonlinear phenomenon known as acoustic radiation pressure. Neglecting viscosity, the Langevin acoustic radiation forceF L imparted to an object (here, the skin) at a focus location is, to second order, given by where p, ρ, and u are the fluid pressure, density, and velocity fields. The angular brackets denote time-averaged quantities, n is the surface normal, I is the unit tensor, and S is a surface region containing the focus location. Subscripts 0, 1, and 2 refer to successive terms in a perturbation expansion about a quiescent fluid configuration (33 –36 ). Applying the same expansion to the Navier-Stokes equation yields an expression for 〈p2〉 in terms of lower-order quantities
Averaged over 1 cycle of oscillation, only the quadratic terms are nonzero. Together, they yield a nonvanishing, low-frequency forceF L(x , t) that elicits vibrations in the skin (see Supplementary Text). The time evolution of these vibrations is governed by a driven elastic wave equation
Here, ξ(x , t) is tissue displacement, and m, μ, and K are the mass density, shear modulus, and bulk modulus, respectively. Plane wave solutions, , describe oscillations along polarization directions . Because of the low-frequency content of the acoustic radiation force and high propagation speed of compression waves, most acoustic energy is transferred to shear (transverse) wave components, . For skin or soft tissues, shear wave speeds, , are frequency dependent and can range from 1 to 10 m/s at the tactile frequencies applicable to this study (22 (link), 23 ). Because of viscoelasticity, wave amplitudes are also attenuated in a frequency-dependent manner with increasing propagation distance (30 (link)).
Ultrasound frequency oscillations cannot be directly felt via touch. In haptic holography, palpable low-frequency mechanical signals are produced via a nonlinear phenomenon known as acoustic radiation pressure. Neglecting viscosity, the Langevin acoustic radiation force
Averaged over 1 cycle of oscillation, only the quadratic terms are nonzero. Together, they yield a nonvanishing, low-frequency force
Here, ξ(
Acoustics
Biological Evolution
Dietary Fiber
Electromagnetic Radiation
Feelings
Holography
Pressure
Skin
Sound
Sound Waves
Tissues
Touch
Transducers
Ultrasonics
Vibration
Viscosity
We examined the relationship between wake formation and tactile motion perception by analyzing a human hand vibrometry dataset and a behavioral dataset that were captured in a previous study by the authors (14 ). In all of the human participant studies, before data collection, written informed consent was gathered for all participants. The experiments were conducted according to the protocol approved by the human participants committee of our institution. The vibrometry experiment measured ultrasound-elicited skin vibrations in the direction normal to the volar (palmar) surface of a human hand using a scanning laser vibrometer (PSV-500, Polytec; sampling rate, 125 kHz). The focused ultrasound haptic display was used in the same manner as in the elastomer experiments. The hand was fixed to a vibration-isolated table in an open posture using 3D-printed brackets affixed to the fingernails via an adhesive, with the arm positioned in a relaxed posture (Fig. 5C ). A constant-intensity source scanned along a 16-cm path to or from the base of the thenar eminence to the tip of digit 2. To facilitate comparison with the perception experiment, we modulated the scanning path in a similar manner to what is commonly used in haptic holography. Thus, the scan path consisted of a zigzag pattern with a 2-cm path width. The focus traversed the nominal motion path (along the path of the finger) at scan speeds vl = 1,2,4,7,11 m/s and was laterally modulated transverse to this primary motion axis, in alternating directions, with velocity vmod = ± 2.5 m/s (see Supplementary Text). For each speed, we analyzed the wake length, or spatial extent of skin vibrations along the motion axis, defined as the length in which vibration amplitude was at least 10% of maximum.
Behavioral data were captured in an experiment on tactile motion perception in which participants reported the direction of scanning via a two-alternative forced choice task. The stimuli were identical to those used in the vibrometry experiment but included an additional low-speed stimulus, vl = 0.5 m/s, whose motion direction could be easily perceived. Scanning stimuli were block-randomized over scanning speed and direction. Each of 12 participants completed 120 trials (10 repetitions for each of 12 stimuli). Participants could elect to repeat the stimuli one or more times. We computed per-participant summary statistics of the response data (Fig. 7B ) and performed statistical analyses using generalized linear mixed modeling with a logistic link function (n = 12; significance of fixed effect coefficients assessed using two-tailed tests). We analyzed combined data from the vibrometry and behavioral experiments to determine the relationship between perception accuracy and wake length (Fig. 7C ).
Behavioral data were captured in an experiment on tactile motion perception in which participants reported the direction of scanning via a two-alternative forced choice task. The stimuli were identical to those used in the vibrometry experiment but included an additional low-speed stimulus, vl = 0.5 m/s, whose motion direction could be easily perceived. Scanning stimuli were block-randomized over scanning speed and direction. Each of 12 participants completed 120 trials (10 repetitions for each of 12 stimuli). Participants could elect to repeat the stimuli one or more times. We computed per-participant summary statistics of the response data (
Elastomers
Epistropheus
Fingernails
Fingers
Holography
Homo sapiens
Motion Perception
Skin
Touch Perception
Ultrasonography
Vibration
Top products related to «Holography»
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More about "Holography"
Holography is a captivating optical technique that uses the interference patterns of light waves to create stunning three-dimensional (3D) images of objects.
This revolutionary technology involves recording the wavefronts of light reflected from an object and then reconstructing the image by illuminating the recorded pattern with a coherent light source, such as a laser.
Holography has a wide array of applications, spanning fields like imaging, data storage, and optical communication.
Researchers can leverage PubCompare.ai, an AI-driven platform, to easily locate relevant protocols from literature, preprints, and patents, and identify the best methods and products for their holography research needs.
This ensures seamless and efficient experimentation on this transformative technology.
Beyond holography, researchers may also find utility in exploring other advanced imaging and visualization tools, such as 3D Cell Explorer, MATLAB, and the EPR finger dewar.
Additionally, components like the IMX081 image sensor and the BX41 and BX40 microscopes can be valuable in holographic and related research.
The use of specialized light sources, such as the Kr ion laser, can also enhance holographic capabilities.
Cutting-edge holographic systems like Q-PHASE and MAX606 offer unique capabilities that can further advance holographic research and applications.
The NRS-3100, a versatile Raman spectrometer, may also prove useful in holographic studies.
By leveraging these complementary technologies and resources, researchers can unlock new frontiers in the captivating world of holography.
This revolutionary technology involves recording the wavefronts of light reflected from an object and then reconstructing the image by illuminating the recorded pattern with a coherent light source, such as a laser.
Holography has a wide array of applications, spanning fields like imaging, data storage, and optical communication.
Researchers can leverage PubCompare.ai, an AI-driven platform, to easily locate relevant protocols from literature, preprints, and patents, and identify the best methods and products for their holography research needs.
This ensures seamless and efficient experimentation on this transformative technology.
Beyond holography, researchers may also find utility in exploring other advanced imaging and visualization tools, such as 3D Cell Explorer, MATLAB, and the EPR finger dewar.
Additionally, components like the IMX081 image sensor and the BX41 and BX40 microscopes can be valuable in holographic and related research.
The use of specialized light sources, such as the Kr ion laser, can also enhance holographic capabilities.
Cutting-edge holographic systems like Q-PHASE and MAX606 offer unique capabilities that can further advance holographic research and applications.
The NRS-3100, a versatile Raman spectrometer, may also prove useful in holographic studies.
By leveraging these complementary technologies and resources, researchers can unlock new frontiers in the captivating world of holography.