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Transits, Gastrointestinal

Transits, Gastrointestinal: The movement of substances or materials through the gastrointestinal tract.
This includes the progression of food, liquids, or other materials from the mouth, through the esophagus, stomach, small and large intestines, and out of the body.
Optimal gastrointestinal transit is crucial for proper digestion, absorption, and elimination.
Disturbances in gastrointestinal transit can lead to a variety of conditions such as constipation, diarrhea, and malabsorption.
Studiyng and understanding gastrointestinal transit is an important aspect of gastrointerology research and clincal practice.

Most cited protocols related to «Transits, Gastrointestinal»

Scintigraphic Evaluation of Gastrointestinal Transit

To evaluate gastrointestinal transit parameters, an adaption of our established scintigraphic method was used [14 (link), 15 (link), 28 (link)]. Briefly, 0.1mCi ¹¹¹InCl₃ was mixed with a slurry of 5 mg of activated charcoal. The mixture was allowed to evaporate to dryness, after which the radiolabeled charcoal was packed into a gelatin capsule. This capsule was coated with one layer of methacrylate (Eudragit L, The Dow Chemical Company) which dissolves in a pH-sensitive manner upon reaching the alkaline terminal ileum, thus allowing radiolabel to be transferred to the colon for quantitation of colon transit. The ¹¹¹In containing capsule was administered following an overnight fast. After this capsule had emptied from the stomach, subjects ingested a ^99mTc-labeled meal. Estimation of colonic filling with ^99mTc at 6 hours (CF6h) served as a surrogate for small bowel transit. Subjects ingested standardized meals for lunch and dinner, 4 and 8 hours after the radiolabeled breakfast, respectively. Using a gamma camera, abdominal images with anterior and posterior cameras of 2 minutes duration were acquired immediately following ingestion of the radiolabeled meal and at specified time points during the subsequent 48 hours period.

Deiteren A., Camilleri M., Bharucha A.E., Burton D., McKinzie S., Rao A, & Zinsmeister A.R. (2009). PERFORMANCE CHARACTERISTICS OF SCINTIGRAPHIC COLON TRANSIT MEASUREMENT IN HEALTH AND IRRITABLE BOWEL SYNDROME AND RELATIONSHIP TO BOWEL FUNCTIONS. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 22(4), 415-e95.

Publication 2009

Abdomen Acclimatization Capsule Charcoal Charcoal, Activated Colon Eudragit L Gamma Cameras Gelatins Ileum Intestines, Small Methacrylate Radionuclide Imaging Transits, Gastrointestinal

Carmine Red Gut Transit Time

Carmine red, which cannot be absorbed from the lumen of the gut, was used to study total GI transit time (Kimball et al., 2005 (link)). A solution of carmine red (300 µl; 6%; Sigma-Aldrich) suspended in 0.5% methylcellulose (Sigma-Aldrich) was administered by gavage through a 21 gauge round-tip feeding needle. The time at which gavage took place was recorded as T₀. After gavage, fecal pellets were monitored at 10 min intervals for the presence of carmine red. Total GI transit time was considered as the interval between T₀ and the time of first observance of carmine red in stool.

Li Z., Chalazonitis A., Huang Y.Y., Mann J.J., Margolis K.G., Yang Q.M., Kim D.O., Côté F., Mallet J, & Gershon M.D. (2011). Essential Roles of Enteric Neuronal Serotonin in Gastrointestinal Motility and the Development/Survival of Enteric Dopaminergic Neurons. The Journal of Neuroscience, 31(24), 8998-9009.

Publication 2011

Carmine Feces Methylcellulose Needles Pellets, Drug Transits, Gastrointestinal Tube Feeding

Gastrointestinal Transit Time Assessment

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Publication 2015

Biological Assay Carmine Feces Fenclonine Methylcellulose Mus Sterility, Reproductive Transits, Gastrointestinal Tube Feeding

Retrospective Gastrointestinal Transit Study

Data were derived in a retrospective manner from a database of previously performed gastrointestinal transit studies conducted in healthy volunteers (see Appendix for references). These volunteers included post-menopausal women and overweight and obese people without other illnesses. All the participants were evaluated by the same research team (gastroenterologist, nurses and coordinators) in a single clinical research unit, and all had clinical evaluation, including physical examination and review of the medical records, to ensure they were healthy and had no disease that could alter gastric emptying. A screening bowel symptom questionnaire using validated questions (5 (link)) was used to exclude significant gastrointestinal symptoms at the times of study.
From this database, subjects participating in studies of pathophysiology or parallel-group design clinical trials were identified; only data obtained after randomization to a placebo group were included.
All participants had provided written consent in each of the previously conducted studies. The current analysis was approved by the Institutional Review Board (IRB) at Mayo Clinic, Rochester, Minnesota. Patients who had withdrawn authorization to use their records for future research purposes had their data removed from the analysis of the current study, as required by the Mayo Clinic IRB.

Camilleri M., Iturrino J., Bharucha A.E., Burton D., Shin A., Jeong I.D, & Zinsmeister A.R. (2012). PERFORMANCE CHARACTERISTICS OF SCINTIGRAPHIC MEASUREMENT OF GASTRIC EMPTYING OF SOLIDS IN HEALTHY PARTICIPANTS. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 24(12), 1076-e562.

Publication 2012

Ethics Committees, Research Gastroenterologist Healthy Volunteers Intestines Nurses Obesity Patients Physical Examination Placebos Postmenopause Transits, Gastrointestinal Voluntary Workers Woman

Intestinal Permeability Evaluation

We recruited 10 healthy volunteers (5 females) who were given instructions to not consume any steroids (6 weeks prior to the testing), medications affecting gastrointestinal transit or permeability (7 days prior to the testing) and artificial sweeteners, lactulose, mannitol (2 days prior to the testing)and during the 24 hour testing period. After baseline urine collection, three saccharides {100 mg ¹³C mannitol, 100 mg ¹²C (regular) mannitol, and 1000 mg lactulose} dissolved in 250 ml of water were administered. Urine collections were pooled for 0-2, 2-8 and 8-24 hours following administration of test sugars and excreted sugar concentrations were measured by HPLC-tandem MS.

Grover M., Camilleri M., Hines J., Burton D., Ryks M., Wadhwa A., Sundt W., Dyer R, & Singh R.J. (2016). 13C Mannitol as a Novel Biomarker for Measurement of Intestinal Permeability. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 28(7), 1114-1119.

Publication 2016

Artificial Sweeteners Carbohydrates Females Healthy Volunteers High-Performance Liquid Chromatographies Lactulose Mannitol Permeability Pharmaceutical Preparations Steroids Sugars Transits, Gastrointestinal Urine Specimen Collection

Most recents protocols related to «Transits, Gastrointestinal»

In vivo Gastrointestinal Transit Assay

Whole gastrointestinal transit time in vivo was detected by applying 6% carmine red solution mingled with 0.5% methylcellulose as reported previously.^{52 (link),53 (link)} In brief, mice without fasted were gavaged with 10 μL/g carmine red solution at 9:00 am, and the time till the appearance of first red fecal pellet was recorded. Partial gastrointestinal motility was detected by measuring the length of red marker peristalsis in total gastrointestinal tract at four timepoint-30 min, 40 min, 60 min and 80 min, respectively, after the carmine red solution gavage.^{54 (link),55 (link)} Blood samples were collected, and gastrointestinal tract was dissected and measured.

Xu Y., Wang J., Wu X., Jing H., Zhang S., Hu Z., Rao L., Chang Q., Wang L, & Zhang Z. (2023). Gut microbiota alteration after cholecystectomy contributes to post-cholecystectomy diarrhea via bile acids stimulating colonic serotonin. Gut Microbes, 15(1), 2168101.

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Publication 2023

BLOOD Carmine Feces Gastrointestinal Motility Gastrointestinal Tract Methylcellulose Mus Peristalsis Suby's G solution Transits, Gastrointestinal Tube Feeding

Gastrointestinal Transit Time in Mice

Gastrointestinal (GI) transit time was assessed immediately following the fourth oral challenge with intragastric OVA, on day 24 after allergic sensitization at ZT3. Mice were gavaged with a 0.25 mL solution with 6% carmine red (C1022, Sigma-Aldrich), 0.5% methylcellulose (M0512, Sigma-Aldrich), and 40 mg grade III OVA. After oral gavage and for the duration of the assay, mice were individually placed in clean cages containing regular bedding. Mice had free access to food and water and were monitored for the occurrence of diarrhoea. The GI transit time was measured as the time between oral gavage and the appearance of the first faecal pellet containing the red carmine dye. Mice were grouped together at the end of the assay.

Florsheim E.B., Bachtel N.D., Cullen J., Lima B.G., Godazgar M., Zhang C., Carvalho F., Gautier G., Launay P., Wang A., Dietrich M.O, & Medzhitov R. (2023). Immune sensing of food allergens promotes aversive behaviour. bioRxiv.

Publication Preprint 2023

Biological Assay Carmine Diarrhea Feces Food Methylcellulose Mice, House Transits, Gastrointestinal Tube Feeding

Fecal Pellet Characterization and GI Transit

Fecal pellet output and water content. Fecal pellets expelled were collected from each animal for 90 minutes during the dark phase on days 5, 12, and 19 just after the bedding change once a week, as well, the water content in feces was assessed (Supplementary Methods).
Whole gastrointestinal transit time. A cohort of experimental mice (n = 20) was administered carmine dye by oral gavaged 2 hours following the last stress, GI transit time was considered as the time between gavage and first observance of a red fecal pellet (Supplementary Methods).

Lobo B., Tramullas M., Finger B.C., Lomasney K.W., Beltran C., Clarke G., Santos J., Hyland N.P., Dinan T.G, & Cryan J.F. (2023). The Stressed Gut: Region-specific Immune and Neuroplasticity Changes in Response to Chronic Psychosocial Stress. Journal of Neurogastroenterology and Motility, 29(1), 72-84.

Publication 2023

2-(2-(2-chloro-3-(2-(3,3-dimethyl-5-sulfo-1-(4-sulfo-butyl)-3H-indol-2-yl)-vinyl)-cyclohex-2-enylidene)-ethylidene)-3,3-dimethyl-1-(4-sulfo-butyl)-2,3-dihydro-1H-indole-5-carboxylic acid Animals Carmine Diarrhea Feces Mice, House Pellets, Drug Transits, Gastrointestinal Tube Feeding

Measuring Stool Water Content and GI Transit

Stool water content was measured in mice as described previously^{75 (link)}. Red carmine dye test was used to measure the total GI tract transit time^{76 (link)}.

Wang H., Chen G., Ahn E.H., Xia Y., Kang S.S., Liu X., Liu C., Han M.H., Chen S, & Ye K. (2023). C/EBPβ/AEP is age-dependently activated in Parkinson’s disease and mediates α-synuclein in the gut and brain. NPJ Parkinson's Disease, 9, 1.

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Publication 2023

Carmine Feces Mice, House Transits, Gastrointestinal

Evaluating GI Transit and Microbiome

The GI transit test was conducted according to the procedure described by Luo et al. [25 (link)], and an activated carbon meal solution was prepared according to the method reported by Wang et al. [26 (link)]. After the 14 d treatment, eight mice were randomly selected from each group and were fasted for 16 h but had free access to water. Then, the mice were orally administered 0.2 mL of the activated carbon meal solution. Thirty minutes later, the mice were sacrificed by cervical dislocation and dissected to obtain the entire small intestine and cecum contents. The total length of the small intestine and the distance covered by the activated carbon meal were measured. The GI transit rate was calculated using Equation (1):

GI transit rate (%) = \frac{distance covered by activated carbon meal (cm)}{total length of the small intestine (cm)} \times 100

Additionally, before intestine dissection, blood samples were collected from the orbits, and the separated serum was used for GI hormone assays. After measuring the intestine’s length, a piece of small intestine tissue about 0.5 cm above the cecum was dissected and fixed in 4% paraformaldehyde for histological observation, and the cecum contents were collected, sealed, and placed on dry ice immediately for gut microbiota analysis.

Liu B., Zhang Z., Liu X., Hu W, & Wu W. (2023). Gastrointestinal Fermentable Polysaccharide Is Beneficial in Alleviating Loperamide-Induced Constipation in Mice. Nutrients, 15(20), 4364.

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Publication 2023

Biological Assay BLOOD Carbon Cecum Charcoal, Activated Dissection Dry Ice Gastrointestinal Microbiome Hormones Intestines Intestines, Small Joint Dislocations Mice, House Neck Orbit paraform Serum Tissues Transits, Gastrointestinal

Top products related to «Transits, Gastrointestinal»

Carmine red by Merck Group

Sourced in United States, Germany, Italy

Carmine red is a natural dye derived from the dried bodies of the female cochineal insects. It is a vibrant red pigment commonly used in various laboratory applications as a coloring agent, stain, and indicator.

Methylcellulose by Merck Group

Sourced in United States, Germany, United Kingdom, Japan, Belgium, Switzerland, Italy, Australia, Canada, China, Sao Tome and Principe, Poland, Israel, France, Spain, Austria

Methylcellulose is a water-soluble, synthetic polymer derived from cellulose. It is a white, odorless, and tasteless powder that is commonly used as a thickening, stabilizing, and emulsifying agent in various industries, including pharmaceutical, food, and personal care products.

Charcoal meal by Merck Group

Sourced in United States, Germany

Charcoal meal is a type of laboratory equipment used for the adsorption of various substances. It is a finely ground form of activated carbon, which has a high surface area and porous structure, allowing it to effectively adsorb a wide range of molecules and compounds.

F 2000 by Hitachi

Sourced in Japan

The F-2000 is a high-performance laboratory spectrofluorometer developed by Hitachi. It is designed to measure the fluorescence properties of various samples, providing accurate and reliable data for researchers and scientists. The F-2000 features advanced optics and detection systems to ensure precise and sensitive measurements.

C1022 by Merck Group

The C1022 is a piece of laboratory equipment manufactured by Merck Group. It is designed to perform a core function within the laboratory setting. The detailed specifications and intended use of this product are not available in this factual and unbiased description.

Non absorbable carmine red dye by Merck Group

Non-absorbable carmine red dye is a synthetic colorant used in various laboratory applications. It is a red pigment derived from carminic acid. This dye is designed to be non-absorbable, meaning it does not penetrate biological tissues or substrates. It is commonly used as a staining agent or tracer in various laboratory procedures and assays.

M0512 by Merck Group

Sourced in United States, United Kingdom, Germany

The M0512 is a piece of laboratory equipment manufactured by Merck Group. It is designed for general laboratory applications. The core function of the M0512 is to perform basic tasks required in a laboratory setting.

Thinkpad w530 by Lenovo

Sourced in Japan

The Lenovo ThinkPad W530 is a professional-grade laptop designed for demanding tasks. It features a 15.6-inch display, Intel Core i7 processor, and NVIDIA Quadro graphics card, making it suitable for tasks such as CAD, video editing, and engineering applications. The device also includes various connectivity options, including USB 3.0 ports, HDMI, and Ethernet.

20g flexible catheter by Terumo

Sourced in Philippines

The 20G flexible catheter is a medical device designed for various laboratory and clinical applications. It features a 20-gauge flexible cannula that can be inserted into the body to facilitate access to specific areas for procedures or sample collection. The catheter is constructed with materials selected for biocompatibility and durability. Its core function is to provide a safe and reliable conduit for accessing targeted sites within the body.

Contour glucometer by Bayer

Sourced in United States, Germany, India, Canada

The Contour glucometer is a handheld device used to measure blood glucose levels. It provides a quick and convenient way for users to monitor their blood sugar levels.

What are the different types of gastrointestinal transits?

Gastrointestinal transits can be categorized into several types, including gastric transit, small intestinal transit, colonic transit, and whole gut transit. Each type refers to the movement of substances through the corresponding section of the gastrointestinal tract. Understanding the specific transit patterns is crucial for evaluating digestive function and identifying potential disturbances.

How can disturbances in gastrointestinal transit lead to health issues?

Disruptions in gastrointestinal transit can contribute to a variety of conditions, such as constipation, diarrhea, malabsorption, and other gastrointestinal disorders. For example, delayed gastric emptying can cause nausea, vomiting, and bloating, while rapid small intestinal transit may lead to malabsorption and nutrient deficiencies. Studying and monitoring gastrointestinal transit is an essential aspect of gastroenterology research and clinical practice.

How can PubCompare.ai assist in optimizing gastrointestinal transit studies?

PubCompare.ai's AI-driven platform can help researchers streamline their studies on gastrointestinal transits in several ways. First, it allows you to efficiently screen the protocol literature to identify the most effective and relevant approaches. Second, the platform's intelligent comparisons can pinpoint critical insights, such as key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy. By leveraging PubCompare.ai, you can optimize your research process and find the most effective protocols for your transits studies.

What are some common usage challenges when studying gastrointestinal transits?

When studying gastrointestinal transits, researchers may face challenges such as selecting the appropriate methodologies, ensuring accurate measurements, and interpreting the results. For example, choosing the right tracer or imaging technique to measure transit times can be crucial for obtaining reliable data. Additionally, factors like diet, medication, and individual variability can impact transit patterns, making it important to carefully design and execute the study protocols. PubCompare.ai can assist in navigating these challenges by helping researchers identify the most effective and validated protocols from the literature.

How can PubCompare.ai help researchers find the best protocols for their gastrointestinal transit studies?

PubCompare.ai's AI-driven platform can be invaluable in helping researchers find the most effective protocols for their gastrointestinal transit studies. By screening the protocol literature more efficiently, the platform can assist in identifying the optimal approaches for your specific research goals. The AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy. This can save time, improve the quality of your research, and ultimately lead to more reliable and impactful findings in the field of gastrointestinal transits.

More about "Transits, Gastrointestinal"

Gastrointestinal transit, also known as GI transit or intestinal transit, refers to the movement of substances, materials, and food through the digestive system.
This crucial biological process involves the passage of contents from the mouth, through the esophagus, stomach, small intestine, and large intestine, before final elimination from the body.
Optimal gastrointestinal transit is essential for proper digestion, absorption, and waste elimination.
Disturbances in GI transit can lead to a variety of gastrointestinal conditions, such as constipation, diarrhea, and malabsorption.
Understanding and studying gastrointestinal transit is a key focus in gastroenterology research and clinical practice.
Researchers and clinicians utilize various techniques, including the use of non-absorbable markers like carmine red dye, methylcellulose, and charcoal meals, as well as advanced technologies like the F-2000 and C1022 systems, to track and analyze GI transit.
The ThinkPad W530 laptop and 20G flexible catheters are also sometimes employed in gastrointestinal transit studies.
Additionally, the Contour glucometer can be used to monitor blood glucose levels, which can provide insights into GI function and absorption.
By leveraging these tools and technologies, researchers can optimize their gastrointestinal transit research protocols and develop more effective approaches to address various digestive health issues.