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Cell Migration Assays

Cell Migration Assays: A critical tool in understanding cell behavior and disease pathogenesis.
These assays measure the directional movement of cells in response to chemical, mechanical, or electrical stimuli.
PubCompare.ai streamlines this process by providing easy access to protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the most effective and reproducible methods.
Optimize your research, achieve accurate results, and advance your understanding of cell migration dynamics.

Most cited protocols related to «Cell Migration Assays»

1
Cell Migration and Invasion Assay
For migration studies, a standard assay was used to determine the number of cells that traversed a porous polycarbonate membrane in response to a chemo-attractant (higher serum concentration)51 (link),52 (link) using the Cytoselect 24-well cell migration assay (Cell Biolabs). Invasion was measured using BD BioCoat Matrigel invasion chambers (BD Bioscience). In both assays, cells were transfected with AMOs, and 1.5 × 105 and 2.5 × 105 cells per well were seeded in an upper chamber in serum free media for the migration and invasion assay, respectively. The lower chamber was filled with media containing 10% FBS. After 24 h, cells passing through polycarbonate membrane were stained and counted according to the manufacturer’s instructions.
Oh J.M., Venters C.C., Di C., Pinto A.M., Wan L., Younis I., Cai Z., Arai C., So B.R., Duan J, & Dreyfuss G. (2020). U1 snRNP regulates cancer cell migration and invasion in vitro. Nature Communications, 11, 1.
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Publication 2020
Biological Assay Cell Migration Assays Cells Culture Media, Serum-Free matrigel polycarbonate Serum Tissue, Membrane
2
Characterization of Signaling Pathways
Detailed methodology can be found in the Supplementary Information. In short, luciferase reporter gene assay, immunoprecipitation and immunoblotting analysis, purification of recombinant proteins, in vitro kinase assays, and cell migration assay were performed, as previously described14 (link),21 (link),22 (link).
Yang W., Xia Y., Ji H., Zheng Y., Liang J., Huang W., Gao X., Aldape K, & Lu Z. (2011). Nuclear PKM2 regulates β-catenin transactivation upon EGFR activation. Nature, 480(7375), 118-122.
Publication 2011
Biological Assay Cell Migration Assays Genes, Reporter Immunoblotting Immunoprecipitation Luciferases Phosphotransferases Recombinant Proteins
3
Proteomic Profiling of Aortic Valve Disease
Detailed methods are provided in the Supplemental Material. The data are available through https://cics.bwh.harvard.edu/multiomics_databases11 .
In total, 25 AVs were used in this study. AV leaflets were obtained from AV replacement surgeries for severe AV stenosis (Brigham and Women’s Hospital (BWH) approved IRB protocol number: 2011P001703). Written informed consent was provided. In brief, human stenotic AVs were segmented into stages of disease progression: (1) non-diseased, (2) fibrotic, and (3) calcific under the guidance of near-infrared molecular imaging. Transition zones were excluded from all analyses. In total, 27 sub-samples were prepared for label-free proteomics and 9 for transcriptomics.
AVs obtained from three additional patients with severe aortic valve stenosis were used for tissue layer tandem mass tagging (TMT) proteomics and AVs from autopsy donors served as controls. Anatomical layer-specificity was facilitated by laser capture microdissection.
Side-specific in vitro layer calcification potential was evaluated through a migration assay on AV leaflets from eight additional patients with severe AV stenosis after inspection by a pathologist to distinguish the fibrosa from the ventricularis side, and calcification was assessed by Alizarin Red staining at day 21. All cells which underwent proteomics were cultured and passaged in vitro prior to protein collection.
AV whole tissue label-free peptide samples were examined with the Q Exactive mass spectrometer. AV tissue layer TMT and in vitro migration label-free peptide samples were analyzed with the LTQ-Orbitrap Elite mass spectrometer.
For pathway analysis, the protein sets corresponding to each layer and stage were tested for enrichment by a hypergeometric test and adjusted for multiple comparisons using the Benjamini-Hochberg method for controlling the false discovery rate (FDR). Pathway networks were constructed based on their gene overlap. Layer- and stage-specific subnetworks were generated from literature-curated physical protein interactions. The closeness of the calcific stage subnetwork to human diseases was evaluated using average shortest network distance.
Schlotter F., Halu A., Goto S., Blaser M.C., Body S.C., Lee L.H., Higashi H., DeLaughter D.M., Hutcheson J.D., Vyas P., Pham T., Rogers M.A., Sharma A., Seidman C.E., Loscalzo J., Seidman J.G., Aikawa M., Singh S.A, & Aikawa E. (2018). Spatiotemporal Multi-omics Mapping Generates a Molecular Atlas of the Aortic Valve and Reveals Networks Driving Disease. Circulation, 138(4), 377-393.
Publication 2018
Aortic Valve Stenosis Autopsy Calcinosis Cell Migration Assays Cells CIC protein, human Disease Progression Donors Fibrosis Gene Expression Profiling Genes Homo sapiens Laser Capture Microdissection Operative Surgical Procedures Pathologists Patients Peptides Physical Examination Proteins Stenosis Tissues Woman
4
Comprehensive Cancer Cell Assays

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Publication 2009
Biological Assay Cell Lines Cell Migration Assays Cell Proliferation Cells Cell Survival Collagen Homo sapiens Malignant Neoplasms matrigel Neoplasms SCID Mice Xenografting
5
Collagen Reconstitution and Cell Migration Assays
Bovine dermal collagen was obtained as acidified, pepsinized solution (stock concentration 3.1 mg/ml; PureCol, Advanced BioMatrix); non-pepsinized (telopeptide intact) rat tail collagen was purified by acid extraction, stored in acidified lyophilized form (Hotary et al., 2000 (link)), and re-solubilized before use in 0.2% acetic acid. Fibrillar collagen matrices were reconstituted in vitro by raising the pH to 7.4 using either final concentrations of 0.345 N NaOH and buffering by 25 µM Hepes (Sigma-Aldrich) for rat tail matrices, or 0.75% Na-bicarbonate solution (Gibco) for bovine dermal lattices, together with mimimum essential Eagle’s medium (Sigma-Aldrich), and polymerized at 37°C, unless indicated otherwise (Hotary et al., 2000 (link); Wolf et al., 2007 (link)). High-density matrices of bovine dermal collagen were reconstituted after concentration of the collagen solution (20 mg/ml; speed vac concentrator; Savant). Delayed polymerization speed of rat tail collagen was achieved by lowering the temperature during polymerization to 20, 14, or 9°C. Cell-free collagen lattices were used for bright-field microscopy (vertical invasion studies; Fig. S1 A), 3D confocal and electron microscopy, and atomic force microscopy. For cell migration assays, cells (20,000/100 µl) after detachment with EDTA (2 mM) or multicellular spheroids (2–7 spheroids/100 µl) were suspended in neutralized collagen solution before polymerization and incorporated into a self-constructed chamber (see Fig. S1 A). After addition of medium (containing 10% FCS) spontaneous migration was monitored, except for PMNs which were stimulated by 100 ng/ml IL-8. Inhibition experiments were performed using 20 µM GM6001, a five-component protease inhibitor cocktail, function-perturbing anti-β1 mAb 4B4, or Y-27632. Inhibitors were added to both the cell–collagen mixture and supernatant. For collagen contraction assays, cells (8 or 16 × 104, for incubation with Y-27632 or 4B4, respectively) were incorporated into non-anchored bovine collagen lattices (300 µl) in 48-well plates.
Wolf K., te Lindert M., Krause M., Alexander S., te Riet J., Willis A.L., Hoffman R.M., Figdor C.G., Weiss S.J, & Friedl P. (2013). Physical limits of cell migration: Control by ECM space and nuclear deformation and tuning by proteolysis and traction force. The Journal of Cell Biology, 201(7), 1069-1084.
Publication 2013
Acetic Acid Acids Bicarbonates Biological Assay Bos taurus Cell Migration Assays Cells Collagen Eagle Edetic Acid Electron Microscopy Fibrillar Collagen GM 6001 HEPES inhibitors Microscopy Microscopy, Atomic Force Polymerization Protease Inhibitors Psychological Inhibition Skin Spheroids, Cellular Tail Wolves Y 27632

Most recents protocols related to «Cell Migration Assays»

1
Generating CXCR3+ Regulatory T-cells
Not available on PMC !

Example 3

CD4+CD45RA+ T-cells are transduced with a lentivirus having nucleic acid sequences encoding a CXCR3 polypeptide and a nucleic acid sequence encoding a FOXP3 polypeptide (FIG. 3). Following transformation, the cells are expanded in the presence of high dose rIL-2 and purified based on low expression of CD127 (IL7 receptor). Other means of purifying the cells as described herein or known in the art can also be used to purify the T-cell. CXCR3 expression is confirmed by flow cytometric analysis using an antibody against the CXCR3 polypeptide (e.g., anti-human CD183 (CXCR3) Biolegend Cat. No. RU0353707). CXCR3 function is also confirmed by an in vitro chemotaxis assay (e.g., transwell migration assay). Briefly, transduced cells are placed in a transwell system with a CXCR3 ligand (e.g., 50-300 ng/mL CXCL10 (human rCXCL10 from R&D Systems Cat No. 266-IP-010)) or a control chemokine present on the side of the membrane opposite the transduced cells. Migration of cells across the membrane is evaluated by flow cytometry using an antibody against CXCR3. Specificity is further confirmed by blocking migration with anti-CXCR3 blocking antibody.

US11879003B2. Methods for increasing T-cell function (2024-01-23). Kyverna Therapeutics, Inc. [US]. Inventors: John Lee [US], Erin O'Brien [US], Jordan Tsai [US], Lih-Yun Hsu [US], Faye Wu [US].
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Patent 2024
Antibodies, Anti-Idiotypic Base Sequence Biological Assay Cardiac Arrest Cell Migration Assays Cells Chemokine Chemokine Receptor Chemotaxis CXCR3 protein, human Flow Cytometry Homo sapiens Immunoglobulins Interleukin 7 Receptor Lentivirus Ligands Migration, Cell Polypeptides T-Lymphocyte Tissue, Membrane Tissues
2
Transwell Migration and Invasion Assay
For the migration assay, 500 μl complete medium was supplemented to the lower chamber of the Transwell chamber, and then 5 × 105 BC cells were resuspended in serum-free medium and subsequently added to the upper chamber of the Transwell chamber (the pore of the Transwell was 8.0 μm, Corning, Corning, NY, USA and Labselect, Chongqing, China). Upon a 24-h incubation in an incubator with 5% CO2, the cells were fixed by 75% ethanol and stained with crystal violet solution. For the invasion experiment, 50 μl of Matrigel diluted by serum-free medium was firstly supplemented into the upper chamber of the Transwell chamber, which was placed at 37 °C for 1 h. The rest steps were the same as the migration experiment.
Wang B., Chen H., Deng Y., Chen H., Xing L., Guo Y., Wang M, & Chen J. (2023). CircDNAJC11 interacts with TAF15 to promote breast cancer progression via enhancing MAPK6 expression and activating the MAPK signaling pathway. Journal of Translational Medicine, 21, 186.
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Publication 2023
Cell Migration Assays Cells Ethanol matrigel Serum Violet, Gentian
3
Transwell Assay for Cell Migration and Invasion
Cell migration and invasion abilities were examined through transwell assay (Corning Costar, USA). For the migration assay, a total of 1 × 105 cells in 100 μl culture medium free of FBS were seeded into the upper chamber, followed by supplementation with 650 ul of culture medium with 10% FBS, in the bottom chamber. Following the incubation for 24 h at 37°C and the removal of the transwell chamber, the samples were rinsed with PBS in triplicate, fixed with 4% paraformaldehyde for 20 min and subsequently stained with 0.5% crystal violet for 20 min. For the invasion assay, the membrane was coated with 50 µl Matrigel to develop a matrix barrier first. Subsequently, the same protocol was employed for transwell assay. Lastly, the number of cells that experienced migration or invasion was observed and calculated in five microscopic fields selected randomly by a microscope (CKX53, Olympus Corp.) at a fixed 200× magnification.
Yu L., Cao H., Yang J.W., Meng W.X., Yang C., Wang J.T., Yu M.M, & Wang B.S. (2023). HDAC5-mediated PRAME regulates the proliferation, migration, invasion, and EMT of laryngeal squamous cell carcinoma via the PI3K/AKT/mTOR signaling pathway. Open Medicine, 18(1), 20230665.
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Publication 2023
Biological Assay Cell Migration Assays Cells Culture Media matrigel Microscopy Migration, Cell paraform Tissue, Membrane Violet, Gentian
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Wound Healing Migration Assay
Control and ASR490-treated ALDH−, ALDH+, and CD44+/CD24− cells were cultured in six-well plates and subjected to wound healing migration assays as described previously (Chandrasekaran et al., 2017 (link)).
Saran U., Chandrasekaran B., Tyagi A., Shukla V., Singh A., Sharma A.K, & Damodaran C. (2023). A small molecule inhibitor of Notch1 modulates stemness and suppresses breast cancer cell growth. Frontiers in Pharmacology, 14, 1150774.
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Publication 2023
CD44 protein, human Cell Migration Assays Cells
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Cell Migration and Invasion Assays
The scratch assay was applied to evaluate cell migration and repair. After reaching 90%–100% confluency in wells of culture plates, cells were exposed to serum-free medium for 6 h, and each cultured well was scraped with a pipette tip in the same specification. Cells were washed with PBS to remove fragments. Microscope images of the same positions were acquired in after 0 and 30 h. Based on the percentage of wound closure area, cell migration was determined.
Transwell migration and invasion assays were conducted to evaluate the ability of cell migration and invasion. 24-well transwell chambers (Corning, United States) were used in the assay. For the invasion assay, matrigel (Corning, United States) was applied to the upper ventricle surface of the basement membrane of the transwell chamber. The insert was filled with 30,000 cells suspended in 150 ul serum-free serum before the assay. In the lower chamber, 700 ul medium containing 12% fetal serum was added for chemotactic stimulation. Cells were cultured for 24 h for migration assays and 40 h for invasion assays. Then cotton swabs were used to remove cells from the surface of the membrane. Cultured cells were fixed with 100% methanol and stained with 0.1% crystal violet. Random visual fields of 3 different inserts were captured, and the number of cells was counted.
After inoculating 3000 cells per well, Huh7 and Hep3B cells were grown for 8 and 10 days respectively in 6 well plates in complete medium. Cultured cells were fixed with 100% methanol and stained with 0.1% crystal violet. Each well was counted for the number of colonies.
Ma Y.L., Yang Y.F., Wang H.C., Yang C.C., Yan L.J., Ding Z.N., Tian B.W., Liu H., Xue J.S., Han C.L., Tan S.Y., Hong J.G., Yan Y.C., Mao X.C., Wang D.X, & Li T. (2023). A novel prognostic scoring model based on copper homeostasis and cuproptosis which indicates changes in tumor microenvironment and affects treatment response. Frontiers in Pharmacology, 14, 1101749.
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Publication 2023
Biological Assay Cell Migration Assays Cells Cerebral Ventricles Cultured Cells Fetus Gossypium matrigel Membrane, Basement Methanol Microscopy Migration, Cell Plasma Membrane Serum Violet, Gentian Wounds

Top products related to «Cell Migration Assays»

1
Matrigel by BD
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
2
Transwell chamber by Corning
Sourced in United States, China, United Kingdom, Germany, Switzerland, Japan, Australia
Transwell chambers are a type of lab equipment used for cell culture and biological assays. They consist of a permeable membrane insert placed inside a well, allowing for the study of cell-cell interactions and the movement of molecules across a barrier. The core function of Transwell chambers is to provide a controlled environment for culturing cells and monitoring their behavior and permeability.
3
Matrigel by Corning
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Netherlands, Montenegro, Switzerland, Austria, Australia, Colombia, Spain, Morocco, India, Azerbaijan
Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
4
Crystal violet by Merck Group
Sourced in United States, Germany, China, Japan, United Kingdom, France, Sao Tome and Principe, Italy, Australia, Macao, Spain, Switzerland, Canada, Belgium, Poland, Brazil, Portugal, India, Denmark, Israel, Austria, Argentina, Sweden, Ireland, Hungary
Crystal violet is a synthetic dye commonly used in laboratory settings. It is a dark purple crystalline solid that is soluble in water and alcohol. Crystal violet has a variety of applications in the field of microbiology and histology, including as a staining agent for microscopy and in the gram staining technique.
5
Transwell insert by Corning
Sourced in United States, United Kingdom, Germany, China, Switzerland, Japan, France, Spain
Transwell inserts are a type of laboratory equipment used for cell culture applications. They consist of a porous membrane that separates two chambers, allowing for the study of interactions between cells or the passage of substances across the membrane. The core function of Transwell inserts is to facilitate the creation of a barrier between the two chambers, enabling researchers to analyze various cellular processes and transport mechanisms.
6
Transwell plate by Corning
Sourced in United States, China, United Kingdom
Transwell plates are a type of cell culture insert system used for in vitro studies. They consist of a permeable membrane support that separates the upper and lower compartments of a well, allowing for the study of cell migration, transport, and co-culture experiments.
7
Transwell chamber by BD
Sourced in United States, China, United Kingdom, Germany, Brazil, Japan
Transwell chambers are a type of cell culture insert used for studying cell migration, invasion, and permeability. They consist of a porous membrane that separates the upper and lower chambers, allowing the passage of cells, molecules, or other materials between the two compartments.
8
Inverted microscope by Olympus
Sourced in Japan, United States, Germany, China, United Kingdom, India, Italy
The Olympus Inverted Microscope is a versatile optical instrument designed for the observation and analysis of samples. It features an inverted configuration, with the objective lenses positioned below the specimen stage, allowing for the examination of cell cultures, microplates, and other samples that require an upright orientation. The inverted design provides a more convenient and accessible workspace for the user.
9
Crystal violet by Beyotime
Sourced in China, United States, Japan, Germany
Crystal violet is a synthetic dye commonly used in laboratory settings. It is a dark purple crystalline solid that is soluble in water and alcohol. Crystal violet is often used as a staining agent in various biological and medical applications, including microscopy and cell identification.

More about "Cell Migration Assays"

Cell migration is a fundamental biological process that plays a crucial role in various physiological and pathological conditions, such as embryonic development, wound healing, immune response, and cancer metastasis.
Cell Migration Assays are a critical tool for researchers to study and understand the mechanisms underlying cell migration.
These assays measure the directional movement of cells in response to chemical, mechanical, or electrical stimuli, providing valuable insights into cell behavior and disease pathogenesis.
PubCompare.ai is a powerful platform that streamlines the process of cell migration research by providing easy access to a vast collection of protocols from literature, preprints, and patents.
The platform leverages advanced AI-driven comparisons to identify the most effective and reproducible methods, allowing researchers to optimize their experiments and achieve accurate results.
Matrigel, a solubilized basement membrane matrix, is a common substrate used in cell migration assays.
These assays often employ Transwell chambers, which consist of a porous membrane insert that separates two compartments.
Cells are seeded in the upper chamber, and their migration through the membrane is measured, typically using Crystal violet staining.
Transwell inserts and plates are widely used in cell migration research to study various aspects of cell behavior, such as chemotaxis, haptotaxis, and mechanotaxis.
Inverted microscopes are commonly used to observe and analyze cell migration in real-time, allowing researchers to track the movement of individual cells and gain valuable insights into the dynamics of cell migration.
By leveraging the tools and resources provided by PubCompare.ai, researchers can streamline their cell migration research, optimize their experimental protocols, and advance their understanding of this crucial biological process.