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Cell Separation

Cell Separation is the process of isolating and purifying specific cell types from a heterogeneous population, such as tissue or blood samples.
This is a critical technique in cell biology, immunology, and regenerative medicine research.
Various methods are used, including centrifugation, immunoaffinity, and microfluidic separation, to enrich for target cells based on physical or molecular properties.
Efficient cell separation enables detailed study of cellular functions, interactions, and applications.
Accurate and reproducible cell separation protocols are essential for advancing our understanding of complex biological systems and developing effective cell-based therapies.

Most cited protocols related to «Cell Separation»

1
Isolation and Characterization of Metastatic Breast Cancer Cells
CN34 tumour cells were isolated from the pleural effusion of a breast cancer patient treated at our institution, after written consent in accordance with Institutional Review Board (IRB) regulations. Brain metastatic populations from these cells and MDA-MB-231cells were obtained by consecutive rounds of in vivo selection in 6–7-week-old beige nude and athymic mice, respectively. All animal work was done in accordance with the MSKCC Institutional Animal Care and Use Committee. Methods for RNA extraction, labelling and hybridization for DNA microarray analysis have been described previously17 (link). Bioinformatics analyses with detailed descriptions can be found in the Methods. Knockdown and overexpression of candidate genes, and cetuximab inhibitor studies were performed as previously described6 (link). The in vitro BBB model was set up as previously described25 (link), and modified to enable tumour cell counting. Sambucus nigra lectin staining was performed using standard histochemical techniques, and quantified using Metamorph software analysis. The Methods section provides further information, including malignant cell isolation from pleural fluids, tumour cell extraction and cell culture protocols, animal inoculation and bioluminescence imaging, generation of retroviral gene knockdown and overexpression vectors, transfections and infections, RNA and protein expression, in vitro BBB transmigration assay, endothelial cell adhesion assay, and metastatic tissue staining and quantification.
Bos P.D., Zhang X.H., Nadal C., Shu W., Gomis R.R., Nguyen D.X., Minn A.J., Van de Vijver M., Gerald W., Foekens J.A, & Massagué J. (2009). Genes that mediate breast cancer metastasis to the brain. Nature, 459(7249), 1005-1009.
Publication 2009
Animals Biological Assay Brain Breast Carcinoma Cell Adhesion Cell Culture Techniques Cells Cell Separation Cetuximab Cloning Vectors Crossbreeding DNA Chips Endothelial Cells Endothelium Ethics Committees, Research Gene Knockdown Techniques Genes Infection Institutional Animal Care and Use Committees Lectin Mice, Nude Microarray Analysis Neoplasms Patients Pleura Pleural Effusion Population Group Proteins Retroviridae Sambucus nigra Tissues Transfection Vaccination
2
SARS-CoV-2 Virus Isolation and Characterization
We used Vero CCL-81 cells for isolation and initial passage. We cultured Vero E6, Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells in Dulbecco minimal essential medium (DMEM) supplemented with heat-inactivated fetal bovine serum (5% or 10%) and antibiotics/antimycotics (GIBCO, https://www.thermofisher.com). We used both NP and OP swab specimens for virus isolation. For isolation, limiting dilution, and passage 1 of the virus, we pipetted 50 μL of serum-free DMEM into columns 2–12 of a 96-well tissue culture plate, then pipetted 100 μL of clinical specimens into column 1 and serially diluted 2-fold across the plate. We then trypsinized and resuspended Vero cells in DMEM containing 10% fetal bovine serum, 2× penicillin/streptomycin, 2× antibiotics/antimycotics, and 2× amphotericin B at a concentration of 2.5 × 105 cells/mL. We added 100 μL of cell suspension directly to the clinical specimen dilutions and mixed gently by pipetting. We then grew the inoculated cultures in a humidified 37°C incubator in an atmosphere of 5% CO2 and observed for cytopathic effects (CPEs) daily. We used standard plaque assays for SARS-CoV-2, which were based on SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV) protocols (9 (link),10 (link)).
When CPEs were observed, we scraped cell monolayers with the back of a pipette tip. We used 50 μL of viral lysate for total nucleic acid extraction for confirmatory testing and sequencing. We also used 50 μL of virus lysate to inoculate a well of a 90% confluent 24-well plate.
Harcourt J., Tamin A., Lu X., Kamili S., Sakthivel S.K., Murray J., Queen K., Tao Y., Paden C.R., Zhang J., Li Y., Uehara A., Wang H., Goldsmith C., Bullock H.A., Wang L., Whitaker B., Lynch B., Gautam R., Schindewolf C., Lokugamage K.G., Scharton D., Plante J.A., Mirchandani D., Widen S.G., Narayanan K., Makino S., Ksiazek T.G., Plante K.S., Weaver S.C., Lindstrom S., Tong S., Menachery V.D, & Thornburg N.J. (2020). Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States. Emerging Infectious Diseases, 26(6), 1266-1273.
Publication 2020
Amphotericin B Antibiotics Atmosphere Biological Assay Cells Cell Separation Cytopathogenic Effect, Viral Dental Plaque Fetal Bovine Serum isolation Middle East Respiratory Syndrome Coronavirus Penicillins SARS-CoV-2 Serum Severe acute respiratory syndrome-related coronavirus Streptomycin Technique, Dilution Tissues Vero Cells Virus
3
Cerebral Organoid Generation from Human Pluripotent Stem Cells
Human H9 ES (WA09) were obtained from WiCell at passage 26 with verified normal karyotype and contamination-free. iPS cells were obtained from System Biosciences (SC101A-1) verified pluripotent and contamination free. All human PSC lines were regularly checked and confirmed negative for mycoplasma. PSCs were maintained on CF-1 gamma irradiated MEFs (Global Stem) according to WiCell protocols. On day 0 of organoid culture, ESCs or iPSCs less than passage 50 were dissociated from MEFs by dispase treatment and MEFs were removed by gravity separation of stem cell colonies from MEFs before trypsinization of stem cells to generate single cells. 4500 cells were then plated in each well of an ultra-low binding 96-well plate (Corning) in hES media with low bFGF (5-fold reduced) and 50uM ROCK inhibitor49 (link) (Calbiochem).
EBs were fed every other day for 6 days then transferred to low adhesion 24-well plates (Corning) in neural induction media containing DMEM/F12, 1:100 N2 supplement (Invitrogen), Glutamax (Invitrogen), MEM-NEAA, and 1ug/ml Heparin50 (link) (Sigma). These began forming neuroepithelial tissues, which were fed every other day for 5 days. On Day 11 of the protocol, tissues were transferred to droplets of Matrigel (BD Biosciences) by pipetting into cold Matrigel on a sheet of Parafilm with small 3mm dimples. These droplets were allowed to gel at 37C and were subsequently removed from the Parafilm and grown in differentiation media containing a 1:1 mixture of DMEM/F12 and Neurobasal containing 1:200 N2 supplement (Invitrogen), 1:100 B27 supplement without vitamin A (Invitrogen), 3.5ul/L 2-mercaptoethanol, 1:4000 insulin (Sigma), 1:100 Glutamax (Invitrogen), 1:200 MEM-NEAA.
After 4 days of stationary growth, the tissue droplets were transferred to a spinning bioreactor containing differentiation media as above except B27 supplement with vitamin A (Invitrogen) was used. Since retinoic acid has been shown to be important for neuronal differentiation in vivo52 (link), we included it in the final media used to differentiate the cerebral organoids.
Lancaster M.A., Renner M., Martin C.A., Wenzel D., Bicknell L.S., Hurles M.E., Homfray T., Penninger J.M., Jackson A.P, & Knoblich J.A. (2013). Cerebral organoids model human brain development and microcephaly. Nature, 501(7467), 10.1038/nature12517.
Publication 2013
2-Mercaptoethanol Bioreactors Cells Cell Separation Common Cold Dietary Supplements dispase Enhanced S-Cone Syndrome Gamma Rays Gravity Homo sapiens Induced Pluripotent Stem Cells Insulin Karyotyping matrigel Mycoplasma Nervousness Neurons Organoids Pancreatic Stellate Cells Stem, Plant Stem Cells Tissues Tretinoin Vitamin A
4
Cell Isolation of Transgenic Worms
Day 1 adult neuronally GFP-labeled worms (Punc119::GFP or Pmec-4::GFP) were prepared for cell isolation as previously described15 (link) with modifications (Extended Data Fig. 2). Synchronized adult worms were washed with M9 buffer to remove excess bacteria. The pellet (~250 µl) was washed with 500 µl lysis buffer (200 mM DTT, 0.25% SDS, 20 mM Hepes pH 8.0, 3% sucrose) and resuspended in 1000 µl lysis buffer. Worms were incubated in lysis buffer with gentle rocking for 6.5 minutes at room temperature. The pellet was washed 6× with M9 and resuspended in 20 mg/ml pronase from Streptomyces griseus (Sigma-Aldrich). Worms were incubated at room temperature (<20 minutes) with periodic mechanical disruption by pipetting every 2 min. When most worm bodies were dissociated, leaving only small debris and eggs, ice-cold PBS buffer containing 2% fetal bovine serum (Gibco) was added. RNA from FACS-sorted neurons was prepared for RNA-seq and subsequent analysis (see Extended Data for details).
Kaletsky R., Lakhina V., Arey R., Williams A., Landis J., Ashraf J, & Murphy C.T. (2015). The C. elegans adult neuronal IIS/FOXO transcriptome reveals adult phenotype regulators. Nature, 529(7584), 92-96.
Publication 2015
Adult Bacteria Buffers Cell Separation Cold Temperature Eggs Fetal Bovine Serum Helminths HEPES Human Body Neurons Pronase RNA-Seq Streptomyces griseus Sucrose
5
Transgenic Mouse Models for Stem Cell Research
Nes-Gfp (ref. 20 (link)), FVB-Tg(Col1a1-cre)1Kry/Mmcd (ref. 29 (link)), B6.Cg(SJL)-TgN(Nes-cre)1Kln (ref. 31 (link)), Nes-CreERT2 (ref. 32 (link)), RCE:loxP (ref. 33 (link)), C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J39 (link), B6.129S4-Gt(ROSA)26Sortm1Sor/J30 (link) transgenic mice (Jackson Laboratories) and C57BL/6-CD45.1 mice (Frederick Cancer Research Center) have been used in these studies. Detailed procedures for cell isolation and culture, in vitro differentiation, LT-CIC and heterotopic bone ossicle assays, histological analyses of ossicles, in vivo treatments and cell depletion experiments, homing assay, RNA isolation, quantitative real-time RT–PCR, gene chip and analyses of microarray experiments, gene ontology and protein–protein interactions are available in the Methods.
Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.
Méndez-Ferrer S., Michurina T.V., Ferraro F., Mazloom A.R., MacArthur B.D., Lira S.A., Scadden D.T., Ma’ayan A., Enikolopov G.N, & Frenette P.S. (2010). Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature, 466(7308), 829-834.
Publication 2010
Biological Assay Bones Cells Cell Separation Ectopic Tissue Gene Chips HBEGF protein, human isolation Malignant Neoplasms Mice, Inbred C57BL Mice, Transgenic Microarray Analysis mitomycin C-dextran Proteins Real-Time Polymerase Chain Reaction Rosa

Most recents protocols related to «Cell Separation»

1
Generation and Maintenance of Haploid Human Embryonic Stem Cells
Not available on PMC !

Example 3

We generated and analyzed a collection of 14 early-passage (passage ≤9) human pES cell lines for the persistence of haploid cells. All cell lines originated from activated oocytes displaying second polar body extrusion and a single pronucleus. We initially utilized chromosome counting by metaphase spreading and G-banding as a method for unambiguous and quantitative discovery of rare haploid nuclei. Among ten individual pES cell lines, a low proportion of haploid metaphases was found exclusively in a single cell line, pES10 (1.3%, Table 1B). We also used viable FACS with Hoechst 33342 staining, aiming to isolate cells with a DNA content corresponding to less than two chromosomal copies (2c) from four additional lines, leading to the successful enrichment of haploid cells from a second cell line, pES12 (Table 2).

Two individual haploid-enriched ES cell lines were established from both pES10 and pES12 (hereafter referred to as h-pES10 and h-pES12) within five to six rounds of 1c-cell FACS enrichment and expansion (FIG. 1C (pES10), FIG. 5A (pES12)). These cell lines were grown in standard culture conditions for over 30 passages while including cells with a normal haploid karyotype (FIG. 1D, FIG. 5B). However, since diploidization occurred at a rate of 3-9% of the cells per day (FIG. 1E), cell sorting at every three to four passages was required for maintenance and analysis of haploid cells. Further, visualization of ploidy in adherent conditions was enabled by DNA fluorescence in situ hybridization (FISH) (FIG. 1F, FIG. 5c) and quantification of centromere protein foci (FIG. 1G, FIG. 5D; FIG. 6). In addition to their intact karyotype, haploid ES cells did not harbor significant copy number variations (CNVs) relative to their unsorted diploid counterparts (FIG. 5E). Importantly, we did not observe common duplications of specific regions in the two cell lines that would result in pseudo-diploidy. Therefore, genome integrity was preserved throughout haploid-cell isolation and maintenance. As expected, single nucleotide polymorphism (SNP) array analysis demonstrated complete homozygosity of diploid pES10 and pES12 cells across all chromosomes.

Both h-pES10 and h-pES12 exhibited classical human pluripotent stem cell features, including typical colony morphology and alkaline phosphatase activity (FIG. 2A, FIG. 2B). Single haploid ES cells expressed various hallmark pluripotency markers (NANOG, OCT4, SOX2, SSEA4 and TRA1-60), as confirmed in essentially pure haploid cultures by centromere foci quantification (>95% haploids) (FIG. 2C, FIG. 7). Notably, selective flow cytometry enabled to validate the expression of two human ES-cell-specific cell surface markers (TRA-1-60 and CLDN618) in single haploid cells (FIG. 2D). Moreover, sorted haploid and diploid ES cells showed highly similar transcriptional and epigenetic signatures of pluripotency genes (FIG. 2E, FIG. 2F). Since the haploid ES cells were derived as parthenotes, they featured distinct transcriptional and epigenetic profiles of maternal imprinting, owing to the absence of paternally-inherited alleles (FIG. 8).

Haploid cells are valuable for loss-of-function genetic screening because phenotypically-selectable mutants can be identified upon disruption of a single allele. To demonstrate the applicability of this principle in haploid human ES cells, we generated a genome-wide mutant library using a piggyBac transposon gene trap system that targets transcriptionally active loci (FIG. 2G, FIG. 8E), and screened for resistance to the purine analog 6-thioguanine (6-TG). Out of six isolated and analyzed 6-TG-resistant colonies, three harbored a gene trap insertion localizing to the nucleoside diphosphate linked moiety X-type motif 5 (NUDT5) autosomal gene (FIG. 2H). NUDT5 disruption was recently confirmed to confer 6-TG resistance in human cells,51 by acting upstream to the production of 5-phospho-D-ribose-1-pyrophosphate (PRPP), which serves as a phosphoribosyl donor in the hypoxanthine phosphoribosyltransferase 1 (HPRT1)-mediated conversion of 6-TG to thioguanosine monophosphate (TGMP) (FIG. 2I). Detection of a loss-of-function phenotype due to an autosomal mutation validates that genetic screening is feasible in haploid human ES cells.

US11859232B2. Screening for chemotherapy resistance in human haploid cells (2024-01-02). YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD. [IL]. Inventors: Nissim Benvenisty [IL].
Patent 2024
Alkaline Phosphatase Alleles Cell Lines Cell Nucleus Cells Cell Separation Centromere Chromosomes Copy Number Polymorphism Diphosphates Diploid Cell Diploidy Embryonic Stem Cells Flow Cytometry Fluorescent in Situ Hybridization Genes Genes, vif Genitalia Genome Genomic Library Haploid Cell HOE 33342 Homo sapiens Homozygote Human Embryonic Stem Cells Hypoxanthine Phosphoribosyltransferase isolation Jumping Genes Karyotype Metaphase Mothers Mutation Nucleosides Oocytes Phenotype Pluripotent Stem Cells Polar Bodies POU5F1 protein, human Proteins purine Ribose Single Nucleotide Polymorphism SOX2 protein, human stage-specific embryonic antigen-4 Tissue Donors Transcription, Genetic
2
Isolation and Characterization of High-Density Neutrophils
Not available on PMC !

Example 21

Isolation of High-Density Neutrophils

10 ml of heparinized (20 U/ml) human blood is mixed with an equal volume of 3% Dextran T500 in saline and incubated for 30 minutes at room temperature to sediment erythrocytes. A 50 ml conical polypropylene tube is prepared with 10 ml sucrose 1.077 g/ml and slowly layered with a leukocyte-rich supernatant on top of the 1.077 g/ml sucrose layer prior to centrifuging at 400×g for 30 minutes at room temperature without brake. The high-density neutrophils (HDN) appear in the pellet. Low-density neutrophils (LDN) co-purify with monocytes and lymphocytes at the interface between the 1.077 g/ml sucrose layer and plasma.

The HDNs may be tested in a CKA assay described herein. Haematopoietic cells are suitably obtained from a donor having HDNs.

US11878033B2. Cancer-killing cells (2024-01-23). LIFT BIOSCIENCES LTD [GB]. Inventors: Alex Blyth [GB], Nico Bruyniks [GB].
Patent 2024
Biological Assay BLOOD Cells Cell Separation Dextran Erythrocytes Hematopoietic System Homo sapiens Leukocytes Lymphocyte Malignant Neoplasms Monocytes Neutrophil Plasma Polypropylenes Retinal Cone Saline Solution Sucrose Tissue Donors
3
Single-Cell RNA-Seq of Rat Skeletal Muscle
Cell isolations were performed as previously described in mice [37 (link)] and modified slightly for rats [28 (link)]. Briefly, the gastrocnemius muscles from WB and HS male rats were excised and placed in muscle dissociation media (MDM) (Hams F-10 (Gibco, USA), 10% Horse Serum (Thermo Fisher), 1% penicillin/streptomycin (Gibco), 800 U/ml Collagenase II (Gibco)), and minced using sterilized surgical equipment. The muscle homogenate was then incubated in MDM for 1 h at 37 °C with gentle agitation. Following incubation, samples underwent further incubation in 1000 U/ml Collagenase II (Gibco) and 11 U/ml dispase (Gibco) for 30 min at 37 °C. The single-cell suspension was passed through an 18-gauge needle approximately 10 times prior to 0.2-μm filtration. Single cells were incubated in propidium iodide to identify dying/dead cells for removal via fluorescence-activated cell sorting (Sony Biotechnology, USA). Single-cell suspensions from each group were added to a Chromium Controller (10X Genomics, USA) using the Single Cell 3’ Reagent Kit per manufacturer’s instructions and sequenced on an Illumina HiSeq platform (Novogene, USA), yielding 200 million reads/sample.
Ismaeel A., Van Pelt D.W., Hettinger Z.R., Fu X., Richards C.I., Butterfield T.A., Petrocelli J.J., Vechetti I.J., Confides A.L., Drummond M.J, & Dupont-Versteegden E.E. (2023). Extracellular vesicle distribution and localization in skeletal muscle at rest and following disuse atrophy. Skeletal Muscle, 13, 6.
Publication 2023
ATF7IP protein, human Cell Separation Chromium Collagenase dispase Equus caballus Filtration Males Mus Muscle, Gastrocnemius Muscle Tissue Needles Penicillins Propidium Iodide Rattus norvegicus Serum Streptomycin Surgical Equipment Type II Mucolipidosis
4
Cultivation of R. toruloides on CGHH and CG
Cultivations were performed as described in [13 (link)]. Briefly, bioreactors (Multifors, Infors HT, Bottmingen, Switzerland) with 500 mL working volume were used for growing R. toruloides CBS14 containing either CGHH (50% CG, 10% HH) or only CG (50% CG) as carbon sources as well as 0.75 g/L yeast extract (BactoTM Yeast Extract, BD, France), 1 g/L MgCl2 6xH2O (Merck KGaA, Germany), 2 g/L (NH4)2HPO4 (≥ 98%, Sigma-Aldrich, USA) and 1.7 g/L YNB without amino acids and ammonium sulphate (DifcoTM, Becton Dickinson, France). To inoculate the bioreactors, cells from a −80 °C stock culture were streaked on YPD-agar (glucose 20 g/L, yeast extract 10 g/L, peptone 20 g/L) and incubated for 3 days. From the plates, cells were inoculated into 100 mL YPD in 500 mL baffled Erlenmeyer flasks. After incubation on an orbital shaker at 125 rpm and 25 °C for 72 h, the cells were transferred to 50 mL-Falcon tubes, washed twice with NaCl-solution (9 g/L) and re-suspended with NaCl-solution. Cultures were inoculated with the cell suspension, to reach an initial OD of 1 in the cultivation. Cultivations were performed in triplicate at 25 °C, pH 6, and an oxygen tension of 21%.
CG was obtained from Perstorp Holding AB, Sweden. The glycerol concentration was specified as 80%; however, there were differences from batch to batch. For the cultivations described here, the same batch was used as in the bioreactor experiments in [13 (link)]. HH was generated from wheat straw, after steam explosion and enzymatic digestion. Pretreatment was performed at Lund University, Sweden, as described previously [16 (link)]. Briefly, the straw was soaked with 1% acetic acid overnight, and then steam exploded at 190 °C. The liquid fraction, representing the solubilised hemicellulose, was removed from the solid fraction by pressing and used in the experiments. HH contained 26.2 g/L xylose, 7 g/L glucose, 6.6 g/L acetic acid and small amounts of arabinose (< 0.5 g/L). The nitrogen content was 0.6 g/l [27 (link)]. The pH was set to 6 by addition of appropriate amounts of 5 M NaOH [13 (link)]. The C/N-ratios were 32.5 for CGHH and 30.7 for CG.
Samples for RNA-isolation and determination of the concentrations of biomass, carbon sources and lipids were isolated from R. toruloides CBS14 cultures grown at different growth conditions: they were taken from CG cultures after 10 h, 30 h, and 60 h and from CGHH cultures after 10 h, 36 h, and 60 h. Cell dry weight was determined gravimetrically, and glucose, xylose, acetic acid and arabinose were determined by HPLC [17 (link)]. Lipid content was measured using FT-NIR, as described previously [28 (link)]. Cell samples for RNA isolation (3 mL) were rapidly collected in Falcon tubes and placed in ice to decrease sample temperature.
Martín-Hernández G.C., Chmielarz M., Müller B., Brandt C., Viehweger A., Hölzer M, & Passoth V. (2023). Enhanced glycerol assimilation and lipid production in Rhodotorula toruloides CBS14 upon addition of hemicellulose primarily correlates with early transcription of energy-metabolism-related genes. Biotechnology for Biofuels and Bioproducts, 16, 42.
Publication 2023
Acetic Acid Agar Amino Acids Arabinose Bioreactors Blast Injuries Carbon Cell Culture Techniques Cells Cell Separation Digestion Enzymes Glucose Glycerin Growth Disorders hemicellulose High-Performance Liquid Chromatographies isolation Lipids Magnesium Chloride Nitrogen Oxygen-21 Peptones Sodium Chloride Steam Sulfate, Ammonium Triticum aestivum Xylose Yeast, Dried
5
Tumor Specimen Processing and Storage
Tumor specimen will be cut into 1-3 mm3 pieces. Depending on the quantity of tumor, different procedures will be carried out in the following order of priority: 1) one random piece will be processed for the isolation of viable cells and PDTO establishment; 2) two random pieces will be snap frozen and stored at − 80 °C for molecular analyses; 3) One random piece will be fixed in paraformaldehyde for paraffin embedding and subsequent histopathological analysis and immunohistochemistry; 4) the remainder will be cryopreserved in freezing solution (10% DMSO, 90% non-inactivated FBS) for future isolation of viable cells. All tumor samples will be stored in the Biological Resource Center ‘Tumorotheque de Caen Basse-Normandie’.
Perréard M., Florent R., Divoux J., Grellard J.M., Lequesne J., Briand M., Clarisse B., Rousseau N., Lebreton E., Dubois B., Harter V., Lasne-Cardon A., Drouet J., Johnson A., Le Page A.L., Bazille C., Jeanne C., Figeac M., Goardon N., Vaur D., Micault E., Humbert M., Thariat J., Babin E., Poulain L., Weiswald L.B, & Bastit V. (2023). ORGAVADS: establishment of tumor organoids from head and neck squamous cell carcinoma to assess their response to innovative therapies. BMC Cancer, 23, 223.
Publication 2023
Biopharmaceuticals Cell Separation Immunohistochemistry Neoplasms paraform Sulfoxide, Dimethyl

Top products related to «Cell Separation»

1
Pan t cell isolation kit by Miltenyi Biotec
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The Pan T Cell Isolation Kit is a laboratory equipment product designed for the isolation of T cells from various sample types. The kit utilizes magnetic bead-based separation technology to enrich for the target T cell population.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Cell mitochondria isolation kit by Beyotime
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The Cell Mitochondria Isolation Kit is a laboratory tool designed to isolate and extract mitochondria from cells. It provides a standardized and efficient method for separating mitochondria from other cellular components, enabling their further analysis and study.
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Nk cell isolation kit by Miltenyi Biotec
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The NK cell isolation kit is a laboratory product designed to isolate natural killer (NK) cells from biological samples. It utilizes magnetic bead-based separation technology to selectively capture and enrich NK cells. The core function of this kit is to provide a reliable and efficient method for the isolation of NK cells for various research and experimental applications.
6
Pan t cell isolation kit 2 by Miltenyi Biotec
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The Pan T Cell Isolation Kit II is a laboratory equipment product designed for the isolation of T cells from various sample types. It utilizes a magnetic bead-based separation method to capture and separate the desired T cell population from the sample.
7
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
8
Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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Rpmi 1640 by Thermo Fisher Scientific
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
10
Fastpure cell tissue total rna isolation kit by Vazyme
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The FastPure Cell/Tissue Total RNA Isolation Kit is a product designed to efficiently extract and purify total RNA from a variety of cell and tissue samples. The kit employs a silica-based membrane technology to capture and elute the RNA, ensuring high-quality RNA suitable for downstream applications such as RT-PCR, Northern blotting, and RNA sequencing.

More about "Cell Separation"

Cell separation is a critical technique in various fields, including cell biology, immunology, and regenerative medicine.
It involves isolating and purifying specific cell types from a heterogeneous population, such as tissue or blood samples.
This process is essential for studying cellular functions, interactions, and applications in depth.
Several methods are used for cell separation, including centrifugation, immunoaffinity, and microfluidic separation.
These techniques enrich for target cells based on their physical or molecular properties.
Efficient cell separation protocols are crucial for advancing our understanding of complex biological systems and developing effective cell-based therapies.
One common tool used in cell separation is the Pan T Cell Isolation Kit, which allows for the purification of T cells from a mixed cell population.
Another important component is fetal bovine serum (FBS), which provides essential nutrients for cell growth and proliferation.
Antibiotics, such as penicillin and streptomycin, are often added to cell culture media to prevent bacterial contamination.
The Cell Mitochondria Isolation Kit is another useful tool for separating and purifying mitochondria from cells, which is important for studying cellular energy metabolism.
The NK cell isolation kit is used to isolate natural killer cells, which play a crucial role in the immune system.
The Pan T Cell Isolation Kit II is a more advanced version of the original Pan T Cell Isolation Kit, offering improved purity and yield of T cells.
Streptomycin and penicillin are commonly used antibiotics that help maintain sterile cell culture conditions.
RPMI 1640 is a widely used cell culture medium that provides a balanced set of nutrients and growth factors for various cell types.
The FastPure Cell/Tissue Total RNA Isolation Kit is a tool for extracting high-quality RNA from cells or tissues, which is essential for gene expression analysis and other molecular biology applications.
Optimizing and reproducing cell separation protocols is a critical aspect of research in these fields.
The PubCompare.ai platform can assist researchers in identifying the most efficient and effective cell separation methods by comparing protocols from the literature, preprints, and patents.
This can help enhance the reproducibility and accuracy of cell separation research, ultimately advancing our understanding of complex biological systems and the development of cell-based therapies.