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Fluoroimmunoassay

Fluoroimmunoassay is a sensitive analytical technique that combines the specificity of immunoassays with the detection capabilities of fluorescence.
This method involves the use of fluorescent-labeled antibodies or antigens to quantify target analytes in biological samples.
Fluoroimmunoassays offer high sensitivity, rapid analysis, and the ability to perform multiplex measurements.
The technique is widely used in various fields, including clinical diagnostics, drug development, and environmental monitoring, to detect and measure a diverse range of biomolecules, such as proteins, hormones, and small molecules.
Streamlining fluoroimmunoassay protocols and optimizing assay conditions can enhance reproducilbity and accuracy, ultimately accelerating research and improving patient outcomes.

Most cited protocols related to «Fluoroimmunoassay»

1
Measurement of MBG and Ouabain Levels
Cited 13 times
For measurement of MBG and endogenous ouabain, samples of plasma and urine were extracted on SepPak C-18 cartridges (Waters, Milford, Massachusetts, USA) as described previously in detail [11 (link)]. The MBG DELFIA fluoroimmunoassays based on anti-MBG 3E9 and 4G4 mAbs were performed as previously described for rabbit anti-MBG polyclonal antibodies [11 (link)]. The assay is based on competition between immobilized antigen (MBG-glycoside-thyroglobulin) and MBG, other cross-reactants, or endogenous CTS within the sample for a limited number of binding sites on an anti-MBG mAbs. Secondary (goat antimouse) antibody labeled with non-radioactive europium was obtained from Perkin-Elmer (Waltham, Massachusetts, USA).
The endogenous ouabain assay was based on a similar principle utilizing an ouabain–ovalbumin conjugate and ouabain antiserum (anti-OU-M-2005; 1 : 20 000) obtained from rabbits immunized with a ouabain-BSA conjugate [20 (link)]. The cross-reactivity of this ouabain antibody is (%) ouabain, 100; ouabagenin, 52, digoxin, 1.8; digitoxin, 0.47; progesterone, 0.002; prednisone, 0.001; proscillaridin, 0.03; bufalin, 0.10; aldosterone, 0.04; telocinobufagin, 0.02; resibufagin, 0.15; marinobufotoxin, 0.06; cinobufagin, 0.02; and MBG, 0.036.
Fedorova O.V., Simbirtsev A.S., Kolodkin N.I., Kotov A.Y., Agalakova N.I., Kashkin V.A., Tapilskaya N.I., Bzhelyansky A., Reznik V.A., Frolova E.V., Nikitina E.R., Budny G.V., Longo D.L., Lakatta E.G, & Bagrov A.Y. (2008). Monoclonal antibody to an endogenous bufadienolide, marinobufagenin, reverses preeclampsia-induced Na/K-ATPase inhibition and lowers blood pressure in NaCl-sensitive hypertension. Journal of hypertension, 26(12), 2414-2425.
Publication 2008
Aldosterone Anti-Antibodies Antigens Binding Sites Biological Assay bufalin Cardiac Glycosides cinobufagin Cross Reactions Digitoxin Digoxin Europium Fluoroimmunoassay Goat Immune Sera Immunoglobulins marinobufotoxin Monoclonal Antibodies Oryctolagus cuniculus ouabagenin Ouabain Ovalbumin Plasma Prednisone Progesterone Proscillaridin Rabbits telocinobufagin Thyroglobulin Urine
2
Offspring of Diabetic Fathers: Growth and Metabolism
Cited 6 times
During 1997–1998, 630 women who completed an oral glucose tolerance test at 30 ± 2 weeks' gestation delivered live, normal babies at the Holdsworth Memorial Hospital, Mysore, India (7 (link)); 41 women had GDM (Carpenter-Coustan criteria) (8 (link)).
At age 5 and 9.5 years, weight (Salter, Kent, U.K.), height (Microtoise; CMS Instruments, Cambridge, U.K.), mid–upper-arm circumference, and triceps and subscapular skinfolds (Harpenden calipers; CMS Instruments) were measured in 514 children available for follow-up (35 ODMs). Systolic and diastolic blood pressure were measured in the left arm (Dinamap; Criticon). Blood samples were collected fasting and 30 and 120 min after a 1.75 g/kg body wt glucose load, after an overnight fast.
Plasma glucose, triglycerides, and HDL cholesterol concentrations were measured by standard enzymatic methods (Alcyon 3000 autoanalyzer; Abbott Laboratories). Insulin was measured using a time-resolved, fluoroimmunoassay (DELFIA) method (PerkinElmer Life 186 and Analytical Sciences, Wallac Qy, Turku, Finland). Interassay coefficients of variations were 12.5% at <45 pmol/l and <10% at ≥45 pmol/l.
Paternal diabetes status was assessed using fasting glucose at the 5-year follow-up. Offspring of non-GDM mothers and diabetic fathers were designated ODFs (n = 39). Offspring of nondiabetic parents were designated control subjects (n = 381). During 6–10 years of age, physical activity was measured in 408 children using Actigraph accelerometers (AM7164/GT1M; MTI) that measure movement in the vertical plane as counts. Detailed methodology is described elsewhere (9 ). Pubertal growth was assessed at age 9.5 years, using breast development in girls and testicular volume in boys (10 ). The hospital ethical committee approved the study; the parents and children gave informed consent/assent.
Krishnaveni G.V., Veena S.R., Hill J.C., Kehoe S., Karat S.C, & Fall C.H. (2009). Intrauterine Exposure to Maternal Diabetes Is Associated With Higher Adiposity and Insulin Resistance and Clustering of Cardiovascular Risk Markers in Indian Children. Diabetes Care, 33(2), 402-404.
Publication 2009
Actigraphy BLOOD Boys Breast Child Diabetes Mellitus Enzymes Fathers Fluoroimmunoassay Glucose High Density Lipoprotein Cholesterol Human Body Infant Insulin Mothers Movement Oral Glucose Tolerance Test Parent Plasma Pregnancy Pressure, Diastolic Puberty Systole Testis TNFSF11 protein, human Triglycerides Woman
3
Effects of Intensive Diabetes Treatment on Kidney Outcomes
Cited 4 times
The DCCT enrolled 1,441 people with T1D from 1983–1989 to determine the effects of INT on the long-term complications of diabetes (7 (link),9 ). The trial included two cohorts. The primary prevention cohort was characterized by diabetes duration 1–5 years, AER <40 mg/24 h, and no retinopathy by fundus photography. The secondary intervention cohort was characterized by diabetes duration 1–15 years, AER ≤200 mg/24 h, and at least one microaneurysm in either eye (but no more than moderate nonproliferative retinopathy). For both cohorts, serum creatinine <1.2 mg/dL or creatinine clearance >100 mL/min/1.73 m2 was required for eligibility. Hypertension, defined as systolic blood pressure ≥140 mmHg, diastolic blood pressure ≥90 mmHg, or treatment with antihypertensive medications, was an exclusion criterion.
Participants were randomly assigned to receive INT or CON, as described in detail elsewhere (7 (link),9 ). The DCCT ended in 1993 after a mean duration of 6.5 years. Subsequently, all surviving participants were invited to join the observational EDIC, which continues today. During EDIC, mean HbA1c levels, which were separated by ∼2% during DCCT, became similar for the two original DCCT treatment groups (8 (link)).
AER was measured yearly during DCCT and every other year during EDIC using supervised 4-h urine collections (10 ,11 (link)). Water intake was encouraged to maintain urine output throughout the collection period. Urine albumin was measured at the DCCT/EDIC Central Biochemistry Laboratory (CBL) at the University of Minnesota using a fluoroimmunoassay (interassay coefficient of variation 8.4%).
Serum creatinine was measured annually throughout DCCT/EDIC at the CBL (interassay coefficient of variation <3%). All results were calibrated to National Institute of Standards and Technology isotope dilution mass spectrometry assigned values (12 (link)). The Chronic Kidney Disease Epidemiology Collaboration equation was used to estimate GFR from calibrated serum creatinine, age, sex, and race (13 (link)).
Blood pressure was measured every 3 months during DCCT and yearly during EDIC. Hypertension was defined as two consecutive study visits with systolic blood pressure ≥140 mmHg, diastolic blood pressure ≥90 mmHg, or the use of antihypertensive medications (14 (link)).
The effects of DCCT INT on AER, estimated GFR, and hypertension were tested according to intention to treat. Hazard ratios (HRs) were estimated using Cox proportional hazards models, which incorporated death as a competing risk when evaluating impaired GFR as the outcome (12 (link)). Risk reduction associated with INT was calculated as (1 − HR of INT vs. CON) × 100%. Proportion of effect explained by DCCT HbA1c was the proportion of INT effect explained by statistical adjustment for the treatment group difference in DCCT mean HbA1c. Cumulative incidence of microalbuminuria or macroalbuminuria by diabetes duration was quantified using a nonparametric estimator that allows for interval-censored observations (15 (link),16 (link)).
, & de Boer I.H. (2013). Kidney Disease and Related Findings in the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications Study. Diabetes Care, 37(1), 24-30.
Publication 2013
Albumins Antihypertensive Agents Blood Pressure Chronic Kidney Diseases Complications of Diabetes Mellitus Creatinine Diabetes Mellitus Eligibility Determination Fluoroimmunoassay High Blood Pressures Isotopes Mass Spectrometry Microaneurysm Pharmacotherapy Pressure, Diastolic Primary Prevention Retinal Diseases Serum Systolic Pressure Technique, Dilution Urine Urine Specimen Collection Water Consumption
4
Anti-MBG Antibody Effects on Dahl-S Rat Hypertension
Cited 4 times
The animal protocol was approved by the Animal Care and Use Committee of the Intramural Research Program, National Institute on Aging. Five-week-old male Dahl-S (SS/JrHsd) rats (n=20) were obtained from Harlan Sprague–Dawley Inc (Indianapolis, Indiana, USA). After adaptation for a week on a low-salt diet (0.2% NaCl; ICN Biochemicals, Irvine, California, USA), rats were placed on an 8% NaCl diet (ICN Biochemicals) and water ad libitum for 4 weeks. During this period, water consumption and urine output were measured weekly. Systolic blood pressure (SBP) was recorded by tail-cuff plethysmography. The effect of anti-MBG antibodies on SBP was studied in Dahl-S rats following 4 weeks of 8% NaCl intake. Anti-MBG 3E9 mAb (n=10) was administered intraperitoneally at a concentration that in vitro reverses the IC75 of MBG-induced inhibition of rat kidney NKA, as described recently in detail (50 μg/kg) [11 (link),12 (link),19 (link)]. Mouse isotype IgG1 control (50 μg/kg; clone 11711, R&D Systems, Inc, Minneapolis, Minnesota, USA) was used as a vehicle (n=10). Following antibody administration, SBP was measured hourly for 3 h. Thereafter, animals were anesthetized with ketamine (100 mg/kg) and sacrificed by exsanguination. Blood samples were collected for measurements of MBG and endogenous ouabain. The serum titers of anti-MBG antibody were determined via solid-phase fluoroimmunoassay as reported previously [20 (link)]. Thoracic aortae were excised for measurements of sodium pump activity (below).
Fedorova O.V., Simbirtsev A.S., Kolodkin N.I., Kotov A.Y., Agalakova N.I., Kashkin V.A., Tapilskaya N.I., Bzhelyansky A., Reznik V.A., Frolova E.V., Nikitina E.R., Budny G.V., Longo D.L., Lakatta E.G, & Bagrov A.Y. (2008). Monoclonal antibody to an endogenous bufadienolide, marinobufagenin, reverses preeclampsia-induced Na/K-ATPase inhibition and lowers blood pressure in NaCl-sensitive hypertension. Journal of hypertension, 26(12), 2414-2425.
Publication 2008
Acclimatization Animals Anti-Antibodies Antibodies, Anti-Idiotypic BLOOD Clone Cells Diet Exsanguination Fluoroimmunoassay IgG1 Immunoglobulin Isotypes Immunoglobulins Ketamine Kidney Low-Sodium Diet Males Mice, House Na(+)-K(+)-Exchanging ATPase Ouabain Plethysmography Psychological Inhibition Rats, Inbred Dahl Serum Sodium Chloride Systolic Pressure Tail Thoracic Aorta Urine Water Consumption
5
Viral Detection in Pediatric Febrile Illness
Cited 4 times
On admission or during hospital stay, a nasal swab was taken for viral studies each time the child had an axillary temperature of ≥38.0°C. The nasal swab sample was obtained through a nostril by inserting a sterile cotton swab to a depth of 2–3 cm and retracting it with rotating movements. The swab was then inserted into a vial containing viral transport medium (5% tryptose phosphate broth, 0.5% bovine serum albumin and antibiotics in phosphate-buffered saline) [9 (link)]. One aliquot of the sample was restored at −70°C. Virus culture was done by using the Ohio strain of HeLa cells and human foreskin fibroblasts according to routine procedure. Viral antigens for respiratory syncytial virus (RSV), adenovirus, parainfluenza virus types 1, 2 and 3, and influenza A and B viruses were detected using a time-resolved fluoroimmunoassay with monoclonal antibodies [1 (link)]. The details of the PCR assays used for rhinovirus, enteroviruses, human herpes virus 6 (HHV-6), coronavirus types OC43 and 229E 52 and human metapneumovirus (hMPV) have been reported earlier [1 (link), 11 (link)]. The observations concerning these respiratory viruses will be reported separately.
Koskenvuo M., Möttönen M., Waris M., Allander T., Salmi T.T, & Ruuskanen O. (2007). Human bocavirus in children with acute lymphoblastic leukemia. European Journal of Pediatrics, 167(9), 1011-1015.
Publication 2007
Adenovirus Infections Antibiotics Antigens, Viral Axilla Biological Assay Child CM 2-3 Coronavirus Infections Enterovirus Fibroblasts Fluoroimmunoassay Foreskin Gossypium HeLa Cells Herpesvirus 1, Cercopithecine Homo sapiens Human Herpesvirus 6 Human Metapneumovirus Influenza Monoclonal Antibodies Movement Nose Para-Influenza Virus Type 1 Phosphates Respiratory Rate Respiratory Syncytial Virus Rhinovirus Saline Solution Serum Albumin, Bovine Sterility, Reproductive Strains tryptose Virus

Most recents protocols related to «Fluoroimmunoassay»

1
Retrospective Study of Newly Diagnosed SLE Patients
Patients newly diagnosed with SLE between January 2009 and December 2019 at a tertiary referral hospital in Seoul, South Korea, were retrospectively included in the study. All patients fulfilled the 2012 Systemic Lupus International Collaborating Clinics (SLICC) classification criteria for SLE20 (link). Patients who were followed up for less than a year were excluded. The retrospective observation period was from the date of SLE diagnosis to the last follow-up date, up to December 2021 or the date of first flare, whichever came first. The following data at the time of diagnosis of SLE were reviewed: age, sex, clinical manifestations of SLE, positivity of anti-dsDNA Ab, anti-Sm Ab, anti-U1RNP Ab, anti-Ro Ab, and anti-La Ab, levels of C3 and C4, and the SLE Disease Activity Index 2000 (SLEDAI-2 K)21 (link). All patients who had symptoms or signs compatible to SLE and were positive for ANA were uniformly tested for anti-dsDNA, anti-Sm, anti-U1RNP, anti-Ro, and anti-La Abs. Therefore, there were no missing data on the positivity for each autoantibody. The positivity of anti-dsDNA Ab and anti-ENA Abs was tested using an automated fluoroimmunoassay analyzer (EliA; Phadia, Uppsala, Sweden). The use of the following medications during the observation period was reviewed: hydroxychloroquine, glucocorticoid, cyclophosphamide, mycophenolate mofetil, azathioprine, methotrexate, cyclosporin, and tacrolimus.
This study was approved by the Institutional Review Board of Gangnam Severance Hospital (No. 3–2020-0114). The requirement for informed consent was waived by the Institutional Review Board of Gangnam Severance Hospital due to the retrospective nature of this study. This study conformed the ethical guidelines laid out by the 1964 Helsinki declaration.
Kwon O.C, & Park M.C. (2023). Risk of systemic lupus erythematosus flares according to autoantibody positivity at the time of diagnosis. Scientific Reports, 13, 3068.
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Publication 2023
Autoantibodies Azathioprine Cyclophosphamide Cyclosporine Diagnosis DNA, Double-Stranded Ethics Committees, Research Fluoroimmunoassay Glucocorticoids Hydroxychloroquine Lupus Vulgaris Methotrexate Mycophenolate Mofetil Patients Pharmaceutical Preparations Tacrolimus
2
Comprehensive Metabolic Profiling for Surgery
One week prior to surgery, the participants were invited to a separate clinical visit for detailed metabolic phenotyping. After an overnight fast, body weight, height, and circumferences of the waist and hip were measured as described (16 (link)). Blood samples were withdrawn from an antecubital vein for measurement of circulating blood count, glucose, insulin, hemoglobin A1C, total cholesterol, high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL) cholesterol, triglyceride, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), and albumin concentrations and for genotyping as described (17 (link)). Plasma glucose concentrations were measured using the hexokinase method, plasma total cholesterol, HDL and LDL cholesterol and triglyceride concentrations were assessed using enzymatic kits and activities of plasma ALT, AST, and GGT were determined using photometric International Federation of Clinical Chemistry methods on an autoanalyzer (Roche Diagnostics Hitachi 917, Hitachi). Serum insulin concentrations were measured by time-resolved fluoroimmunoassay using an Insulin Kit (AUTOdelfia, Wallac). Hepatic insulin resistance was assessed by using the HOMA-IR, calculated using the following formula: serum insulin (mU/L) × plasma glucose (mmol/L)/22.5 (18 (link)). Hemoglobin A1C was determined using an immunoturbidometric method (Abbott Laboratories). Blood counts and platelets were measured using impedance, flow cytometric and photometric assays (XN10, Sysmex). Plasma alb min concentrations were determined by using a photometric method on an autoanalyzer (Modular Analytics EVO, Hitachi). The genotyping of HSD17B13 rs72613567, PNPLA3 rs738409, TM6SF2 rs58542926, MBOAT7 rs641738, and MARC1 rs2642438 was performed using the TacMan polymerase chain reaction method (Applied Biosystems) (17 (link)).
Luukkonen P.K., Sakuma I., Gaspar R.C., Mooring M., Nasiri A., Kahn M., Zhang X.M., Zhang D., Sammalkorpi H., Penttilä A.K., Orho-Melander M., Arola J., Juuti A., Zhang X., Yimlamai D., Yki-Järvinen H., Petersen K.F, & Shulman G.I. (2023). Inhibition of HSD17B13 protects against liver fibrosis by inhibition of pyrimidine catabolism in nonalcoholic steatohepatitis. Proceedings of the National Academy of Sciences of the United States of America, 120(4), e2217543120.
Publication 2023
Albumins Aspartate Transaminase Biological Assay BLOOD Blood Platelets Body Weight Cholesterol Cholesterol, beta-Lipoprotein D-Alanine Transaminase Diagnosis Enzymes Flow Cytometry Fluoroimmunoassay gamma-Glutamyl Transpeptidase Glucose Hemoglobin A, Glycosylated Hexokinase High Density Lipoprotein Cholesterol High Density Lipoproteins Immunoturbidimetry Insulin Insulin Resistance Low-Density Lipoproteins Operative Surgical Procedures Photometry Plasma Polymerase Chain Reaction Serum Triglycerides Veins Waist Circumference
3
Biomarker Analysis in Serum
Inflammatory cytokines and adipokines will be measured in serum using high-sensitivity sandwich ELISA. Total testosterone will be measured using liquid chromatography-tandem mass spectrometry (sensitivity of 2 ng/dL [0.0693 nmol/L]), and free testosterone will be measured with equilibrium dialysis. Sex hormone binding globulin will be measured by solid phase immunofluorometric assay. Except for sex steroids (which will be measured in real time), inflammatory cytokines and adipokines will be measured on frozen plasma and sera will be banked in a -80°C freezer.
Braga-Basaria M., Travison T.G., Taplin M.E., Lin A., Dufour A.B., Habtemariam D., Nguyen P.L., Kibel A.S., Ravi P., Bearup R., Kackley H., Kafel H., Reid K., Storer T., Simonson D.C., McDonnell M, & Basaria S. (2023). Gaining metabolic insight in older men undergoing androgen deprivation therapy for prostate cancer (the ADT & Metabolism Study): Protocol of a longitudinal, observational, cohort study. PLOS ONE, 18(2), e0281508.
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Publication 2023
Adipokines Cytokine Dialysis Enzyme-Linked Immunosorbent Assay Fluoroimmunoassay Freezing Gonadal Steroid Hormones Hypersensitivity Inflammation Liquid Chromatography Plasma Serum Sex Hormone-Binding Globulin Tandem Mass Spectrometry Testosterone
4
Oral Glucose Tolerance Test and Metabolic Measures
The oral glucose tolerance test (OGTT) consisted of 75 g of glucose administered orally after three days of a carbohydrate-rich diet; blood samples for glucose and insulin measurements were drawn before administration of the glucose solution and 30, 60, 90, and 120 min after administration.
Plasma glucose concentration was determined using the glucose oxidase method [24 (link)]. All lipid measurements were obtained directly from plasma samples. The total cholesterol and triglyceride levels were assessed using enzymatic methods (Roche Laboratories). HDL-C was quantified using the same method, and LDL-C was estimated using the Friedwald formula [25 (link)]. Glycated hemoglobin was measured using high-performance liquid chromatography [25 (link)] in a centrifuge. Progesterone was measured using an immunofluorometric assay (Wallac, Helsinki, Finland) using Auto DELFIA kits; androstenedione, prolactin (PRL), luteinizing hormone (LH), and follicular stimulating hormone (FSH), by the immunofluorometric assay; dehydroepiandrosterone sulfate (DHEAS), by radioimmunoassay (Cisbio International, Saclay, France, and DSL, Houston, TX, USA); insulin and 17-OHP, by radioimmunoassay using DSL kits. Testosterone and sex hormone-binding globulin (SHBG) levels were measured using an electrochemiluminescent immunoassay (Modular; Roche). The free testosterone index was calculated using the following formula: total testosterone/SHBG × 100. Free testosterone levels were calculated using the Vermeulen formula. All analyses were performed twice, and the intra- and inter-assay coefficients of variation did not exceed 10% and 15%, respectively.
Baracat M.C., Baracat E.C., Simões R.S., Simões M.J., Maciel G.A., Azziz R, & Soares JM J.r. (2023). Hormonal and Metabolic Factors Influence the Action of Progesterone on the Endometrium of Women with Polycystic Ovary Syndrome. Diagnostics, 13(3), 382.
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Publication 2023
Androstenedione Biological Assay Blood Glucose Cholesterol Dehydroepiandrosterone Sulfate Dietary Carbohydrates Enzymes Fluoroimmunoassay Follicle-stimulating hormone Glucose Hemoglobin, Glycosylated High-Performance Liquid Chromatographies Immunoassay Insulin Lipids Luteinizing hormone Oral Glucose Tolerance Test Oxidase, Glucose Plasma Progesterone Prolactin Radioimmunoassay Sex Hormone-Binding Globulin Testosterone Triglycerides
5
Autoimmune Biomarker Evaluation Protocol
Anti-CCP antibody levels were measured by immunofluorimetric assay and considered positive if the value >10 EliA U/ml. Rheumatoid factor (RF) was measured by the latex agglutination technique and was considered reactive if >8 IU/mL. Antinuclear antibody (ANA) was evaluated by indirect immunofluorescence with HEp-2 cells as substrate, and a titer ≥ 1:80 was considered positive. The erythrocyte sedimentation rate (ESR) in the first hour was determined using the Westergren method and was considered normal up to 20 mm. High-sensitivity C-reactive protein (CRP) was evaluated by immunoturbidimetry, and reference values up to 8 mg/L were considered normal.
Bessa E.J., Ribeiro F.D., Rodrigues R.S., Henrique da Costa C., Rufino R., Pinheiro G.D, & Lopes A.J. (2023). Association between clinical, serological, functional and radiological findings and ventilatory distribution heterogeneity in patients with rheumatoid arthritis. PLOS ONE, 18(10), e0291659.
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Publication 2023
Antibodies, Anti-Idiotypic Antibodies, Antinuclear Cells C Reactive Protein Fluoroimmunoassay Immunoturbidimetry Indirect Immunofluorescence Latex Fixation Tests Rheumatoid Factor Sedimentation Rates, Erythrocyte

Top products related to «Fluoroimmunoassay»

1
Autodelfia by PerkinElmer
Sourced in Finland, United States, United Kingdom, Norway
The AutoDELFIA is a fully automated immunoassay system designed for high-throughput testing. It offers accurate and reliable detection of analytes in biological samples. The system is capable of performing a wide range of immunoassays, providing laboratories with a versatile and efficient solution for their testing needs.
2
Sep pak c18 cartridge by Waters Corporation
Sourced in United States, Italy, Ireland, United Kingdom, Canada, Germany
The Sep-Pak C18 cartridge is a solid-phase extraction (SPE) device used for sample preparation in analytical chemistry. It is designed to selectively retain and concentrate analytes of interest from complex matrices prior to instrumental analysis.
3
Cobas e601 by Roche
Sourced in Germany, Switzerland, United States, China, Sweden, United Kingdom, Japan, Canada
The Cobas e601 is an automated immunochemistry analyzer used for in vitro diagnostic testing. It is designed to perform a wide range of immunoassay tests, including those for hormones, tumor markers, and infectious diseases. The Cobas e601 utilizes electrochemiluminescence technology to provide accurate and reliable results.
4
Immulite 2000 by Siemens
Sourced in Germany, United States, United Kingdom, Italy, Spain, Japan
The Immulite 2000 is an automated immunoassay analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of immunoassay tests, including those for hormones, proteins, and other analytes. The system utilizes chemiluminescent technology for detection and provides automated sample handling, reagent management, and result reporting.
5
Wallac 1235 autodelfia by PerkinElmer
Sourced in United States
The Wallac 1235 AutoDELFIA is a compact and fully automated fluoroimmunoassay system designed for high-throughput clinical laboratory testing. The system automates the complete assay workflow, from sample handling to results reporting, ensuring reliable and consistent performance.
6
Auto delfia 1235 by PerkinElmer
Sourced in United States, Finland
The Auto DELFIA 1235 is a fluorescence-based immunoassay instrument designed for automated and high-throughput sample processing. It is capable of performing a variety of immunoassay procedures, including time-resolved fluorescence (TRF) and fluorescence resonance energy transfer (FRET) techniques.
7
Autodelfia analyser by PerkinElmer
Sourced in United States, Finland
The AutoDELFIA analyser is a fully automated and high-throughput immunoassay system designed for clinical laboratory applications. It is capable of performing a wide range of immunoassay tests, including those for hormones, tumor markers, and infectious disease markers. The AutoDELFIA analyser offers efficient sample handling, reliable and reproducible results, and automated data processing.

More about "Fluoroimmunoassay"

Fluoroimmunoassay (FIA) is a highly sensitive analytical technique that combines the specificity of immunoassays with the detection power of fluorescence.
This method involves the use of fluorescent-labeled antibodies or antigens to quantify target analytes, such as proteins, hormones, and small molecules, in biological samples.
FIA offers several advantages, including high sensitivity, rapid analysis, and the ability to perform multiplex measurements, making it widely used in various fields like clinical diagnostics, drug development, and environmental monitoring.
Streamlining FIA protocols and optimizing assay conditions can enhance reproducibility and accuracy, ultimately accelerating research and improving patient outcomes.
Platforms like PubCompare.ai utilize AI-driven comparisons to help researchers locate the best FIA protocols from literature, preprints, and patents, and optimize their workflows.
Other related techniques and instruments used in FIA include AutoDELFIA, a time-resolved fluoroimmunoassay system; Sep-Pak C18 cartridges for sample preparation; Cobas e601, a fully automated immunoassay analyzer; Immulite 2000, a chemiluminescent immunoassay system; and Wallac 1235 AutoDELFIA, a time-resolved fluorescence immunoassay analyzer.
These tools and technologies can be leveraged to enhance the efficiency and accuracy of FIA-based research and diagnostics.