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Immunomagnetic Separation

Immunomagnetic Separation is a powerful technique used to isolate and purify specific cell types, molecules, or pathogens from complex mixtures.
This process involves the use of magnetic beads coated with antibodies or ligands that bind to the target of interest.
The magnetically-labeled targets can then be separated from the rest of the sample using a magnetic field, allowing for highly selective and efficient isolation.
Immunomagnetic Separation is widely used in biomedical research, diagnostics, and therapeutic applications, such as the isolation of circulating tumor cells, stem cells, or infectious agents.
This versatile method enables researchers to achieve high purity and recovery rates, while maintaining the integrity of the separated targets.
With its reproducibility and accuracy, Immunomagnetic Separation is an essential tool for advancing scientific discoveries and improving patient care.

Most cited protocols related to «Immunomagnetic Separation»

1
HHV-6 Variant Identification and Transcription Analysis
DNA and RNA were isolated from clinical samples and Nthy-ori3-1 cells as described [27] (link). All RNA preparations were devoid of DNA, as assured by multiple DNase digestions and lack of amplification in PCR reactions where retrotranscription (RT) had been omitted [14] (link). HHV-6 DNA presence and load were analyzed by PCR and real time quantitative (qPCR) specific for the U94 and U42 genes [27] (link), and samples were considered positive when 1 µg of cell DNA harbored more than 100 copies of viral DNA [27] (link). Amplification of the house-keeping human RNase P gene was used as a control. All clinical samples were analyzed in a randomized and blinded fashion. In addition, 15/28 control and 21/34 HT FNAs, when there was enough material to repeat the analysis, were tested again in a randomized and blinded fashion at a distant time from the first analyses.
HHV-6 variant A or B identification was obtained by restriction enzyme digestion with HindIII enzyme of the U31 nested PCR amplification product, as reported previously [26] (link). Digestion products were then visualized on ethidium bromide stained agarose gel after electrophoresis migration.
Virus transcription was assessed by PCR or qPCR after retrotranscription (RT-PCR, RT-qPCR), determining the presence of lytic (U42, U22) or latent (U94 in the absence of U42) mRNAs, as previously reported [27] (link). The sensitivity of the used PCRs was similar for all genes, detecting as few as 100 copies of target sequence.
Cell fractions derived by immunomagnetic separation of FNAs were characterized by RT-PCR specific for leukocytes transcripts (respectively CD45, CD3), using serial dilutions of cDNA template, corresponding to amounts of total extracted RNA ranging from 100 ng to 1 pg. Primers and PCR conditions for CD3 and CD45 were previously reported [53] (link), [54] (link), and amplification reactions were carried out for 30 cycles. In each assay the cDNAs obtained from JJhan T cells or Nthy-ori3-1 thyroid cells were also included as positive and negative controls respectively. Amplification of the house-keeping β-actin gene was used as a control.
Caselli E., Zatelli M.C., Rizzo R., Benedetti S., Martorelli D., Trasforini G., Cassai E., degli Uberti E.C., Di Luca D, & Dolcetti R. (2012). Virologic and Immunologic Evidence Supporting an Association between HHV-6 and Hashimoto's Thyroiditis. PLoS Pathogens, 8(10), e1002951.
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Publication 2012
Actins Aspiration Biopsy, Fine-Needle Biological Assay Cells Deoxyribonucleases Digestion DNA, Complementary DNA, Viral DNA Restriction Enzymes Electrophoresis, Agar Gel Enzymes Ethidium Bromide Gene Amplification Genes Homo sapiens Human Herpesvirus 6 Hypersensitivity Immunomagnetic Separation Leukocytes Oligonucleotide Primers RNA, Messenger RNase P T-Lymphocyte Technique, Dilution Thyroid Gland Viral Transcription
2
Quantifying HIV-1 Proviral Reservoir by ddPCR
To evaluate the size of the proviral reservoir, CD4+ T cells were purified from PBMC by negative immunomagnetic separation (CD4+ T Cell Isolation Kit; Miltenyi Biotech), and lysed extracts were used to measure cell-associated total HIV-1 DNA by droplet digital polymerase chain reaction (ddPCR) with 5′LTR or Gag primers and probes, depending on the efficiency of detection in each patient. The 2 primer-probe sets have been previously assessed to be comparable in terms of efficiency and sensitivity by ddPCR on a plasmid containing the IIIB reference HIV-1 sequence (standard 2LTR-CCR5 plasmid, kindly provided by M. Stevenson). However, mismatches or deletions in the viral sequences can prevent from efficient amplification in some patient samples. For that reason all samples were measured in parallel using the 2 primer-probe sets to ensure efficient and reliable proviral absolute quantification. The RPP30 cellular gene was quantified in parallel to normalize sample input. All primers and FAM/HEX-ZEN-Iowa BlackFQ dual-labeled double-quenched probes were purchased from Integrated DNA Technologies [28 (link), 29 (link)].
Martínez-Bonet M., Puertas M.C., Fortuny C., Ouchi D., Mellado M.J., Rojo P., Noguera-Julian A., Muñoz-Fernández M.A, & Martinez-Picado J. (2015). Establishment and Replenishment of the Viral Reservoir in Perinatally HIV-1-infected Children Initiating Very Early Antiretroviral Therapy. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 61(7), 1169-1178.
Publication 2015
CCR5 protein, human CD4 Positive T Lymphocytes Cells Fingers Genes HIV-1 Hypersensitivity Immunomagnetic Separation isolation Oligonucleotide Primers Patients Plasmids Polymerase Chain Reaction Proviruses Sequence Deletion
3
Identifying Subtyping Marker for C. ubiquitum
To identify a subtyping marker for C. ubiquitum, we sequenced the genome of an isolate from a specimen (33496) from a Verreaux’s sifaka by 454 technology using a GS FLX+ System (454 Life Sciences, Branford, CT, USA). This specimen was selected for whole-genome sequencing because of the high number of oocysts present, the availability of ample fecal materials for isolation of oocysts by sucrose and cesium chloride gradient flotation and immunomagnetic separation, and minor contamination from nontarget organisms in extracted DNA. Of the 3,030 assembled contigs of 11.4 MB nucleotides generated from 1,069,468 sequence reads, 1contig (no. 0067), consisting of 45,014 bp, had a high sequence similarity to the 5′ and 3′ ends of the gp60 gene and the flanking intergenic regions. Alignment of the contig 0067 sequence with the nucleotide sequences of the C. parvum gp60 gene (AF203016 and AY048665) led to the identification of sequences conserved between C. ubiquitum and C. parvum, which were used to design a nested PCR that amplified the entire coding region of the gp60 gene, except for the 54 nt at the 3′ end. The sequences of primers used in primary and secondary PCR were 5′-TTTACCCACACATCTGTAGCGTCG-3′ (Ubi-18S-F1) and 5′-ACGGACGGAATGATGTATCTGA-3′ (Ubi-18S-R1), and 5′-ATAGGTGATAATTAGTCAGTCTTTAAT-3′ (Ubi-18S-F2) and 5′-TCCAAAAGCGGCTGAGTCAGCATC-3′ (Ubi-18S-R2), which amplified an expected PCR product of 1,044 and 948 bp, respectively.
Li N., Xiao L., Alderisio K., Elwin K., Cebelinski E., Chalmers R., Santin M., Fayer R., Kvac M., Ryan U., Sak B., Stanko M., Guo Y., Wang L., Zhang L., Cai J., Roellig D, & Feng Y. (2014). Subtyping Cryptosporidium ubiquitum,a Zoonotic Pathogen Emerging in Humans. Emerging Infectious Diseases, 20(2), 217-224.
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Publication 2014
Base Sequence cesium chloride Feces Genes Genome Immunomagnetic Separation Intergenic Region isolation Nested Polymerase Chain Reaction Nucleotides Oligonucleotide Primers Oocysts Propionibacterium acnes Sequence Alignment Sucrose
4
Isolation and Characterization of Lung Lymphocytes
Lung lymphocytes were isolated by modifying established protocols, using a combination of mechanical fragmentation, enzyme digestion, and centrifugation procedures described previously [35 (link),36 (link),37 (link)]. Viable lymphocytes were separated from whole lung inflammatory cells (macrophages, eosinophils, and neutrophils) using an immunomagnetic positive separation technique (autoMACS, Miltenyi Biotec, Auburn, California, United States). Briefly, lung leukocytes were labeled with paramagnetic bead-conjugated anti-CD3, -CD19, and -CD56 to positively select T, B, and NK cells, according to the manufacturer's instructions. Each of the harvested cell populations was used directly for in vitro assays or was cryopreserved in aliquots of 1 × 107 cells for future analysis.
Grumelli S., Corry D.B., Song L.Z., Song L., Green L., Huh J., Hacken J., Espada R., Bag R., Lewis D.E, & Kheradmand F. (2004). An Immune Basis for Lung Parenchymal Destruction in Chronic Obstructive Pulmonary Disease and Emphysema. PLoS Medicine, 1(1), e8.
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Publication 2004
Biological Assay Cells Centrifugation Digestion Enzymes Eosinophil Immunomagnetic Separation Leukocytes Lung Lymphocyte Macrophage Muromonab-CD3 Natural Killer Cells Neutrophil Pneumonia Population Group
5
Isolation of Umbilical Cord Blood Stem Cells
Umbilical cord blood samples were lysed in BD PharmLyse Lysing Solution (BD Biosciences, San Jose, CA, USA) for 15 min at room temperature in the dark and washed twice in phosphate–buffered saline (PBS). The obtained suspension of UCB nucleated cells (NCs) was subjected to immunomagnetic separation procedures. Each cell population, lineage-negative SPCs, CD34+ or CD133+ cells enriched in SPCs were isolated from non-separated NCs using immunomagnetic isolation and a Lineage Cell Depletion Kit, CD34 MicroBead Kit or CD133 MicroBead Kit (Miltenyi Biotec, Auburn, CA, USA). Isolation procedures were performed according to the manufacturer's instructions. Briefly, lineage-negative cells were isolated through negative selection using a MidiMACS separator (Miltenyi Biotec, Auburn, CA, USA). To isolate a lineage-negative cell population, a Lineage Cell Depletion Kit (Miltenyi Biotec, Auburn, CA, USA) was used. One hundred microliters of biotin-antibody cocktail recognizing the lineage-specific cell antigens were added per 108 cells according to the manufacturer's recommendations. After washing in PBS, 100 µL of anti-biotin MicroBeads for magnetic cell labeling was added. Labeled cell suspension was loaded onto a MACS LS column (Miltenyi Biotec), and unlabeled cells passing through the column were collected (lineage-negative population).
CD34+ and CD133+ cells were isolated through positive selection using a MidiMACS separator (Miltenyi Biotec). To label CD34+ cells, a CD34 MicroBead Kit was used, and CD133+ cells were labeled using the CD133 MicroBead Kit (Miltenyi Biotec). One hundred microliters of FcR blocking reagent, which inhibits nonspecific or Fc-receptor-mediated binding, and 100 µL of CD34/CD133 microbeads for magnetic cell labeling were added per 108 cells according to the manufacturer's recommendations. Labeled cell suspensions were subjected to immunomagnetic separation, in which magnetically labeled cells were retained in the LS MACS affinity column (Miltenyi Biotec) and unlabeled cells passed through the column. After several washes, the column was removed from the magnet, and the retained CD34+ or CD133+ cells were eluted with 1-5 mL of PBS supplemented with 0.5% bovine serum albumin and 2 mM EDTA.
From UCB units with relatively high volumes (at least 450×109 NCs), we simultaneously isolated three SPC populations immunomagnetically, but from units with a lower UCB volume, we only isolated lineage-negative SPCs for a more detailed analysis. The isolation procedures were performed immediately after obtaining NCs from the UCB units. From the 56 UCB units processed, lineage-negative cells were isolated 56 times, and CD34+ and CD133+ cells were isolated 25 times. Total numbers of isolated cells were determined using a TC Automated Cell Counter (Bio-Rad Inc., Philadelphia, PA, USA).
Paczkowska E., Kaczyńska K., Pius-Sadowska E., Rogińska D., Kawa M., Ustianowski P., Safranow K., Celewicz Z, & Machaliński B. (2013). Humoral Activity of Cord Blood-Derived Stem/Progenitor Cells: Implications for Stem Cell-Based Adjuvant Therapy of Neurodegenerative Disorders. PLoS ONE, 8(12), e83833.
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Publication 2013
Antigens Biotin Cardiac Arrest Cells Combined Antibody Therapeutics Edetic Acid Fc Receptor Immunomagnetic Separation Microspheres Phosphates Population Group Saline Solution Serum Albumin, Bovine Umbilical Cord Blood

Most recents protocols related to «Immunomagnetic Separation»

1
Inhibition of Heat Shock Proteins in Human B Cells
Human peripheral blood mononuclear cells (PBMC) were separated by Ficoll-Paque gradient centrifugation (Lympholyte; Cedarlane, CL5020) from buffy coats of healthy donors. B lymphocytes were isolated from PBMCs by immunomagnetic cell separation, using anti-CD19-conjugated microbeads, according to the manufacturer’s instructions (Miltenyi Biotec, 130–050-301), and were plated in 6-well plates at a density of density of 4 × 106 per well, in a final volume of 2 mL in MC, and treated for 24 h with J2 (20 µM), PES (20 µM) and 17-AAG (1 µM), inhibitors of HSP27, HSP70 and HSP90, respectively.
Gonnella R., Arena A., Zarrella R., Gilardini Montani M.S., Santarelli R, & Cirone M. (2023). HSPs/STAT3 Interplay Sustains DDR and Promotes Cytokine Release by Primary Effusion Lymphoma Cells. International Journal of Molecular Sciences, 24(4), 3933.
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Publication 2023
B-Lymphocytes Centrifugation Donors Ficoll Heat-Shock Proteins 70 Heat Shock Protein 27 Homo sapiens HSP90 Heat-Shock Proteins Immunomagnetic Separation inhibitors Microspheres PBMC Peripheral Blood Mononuclear Cells tanespimycin
2
Autologous Tumor Lysate-Pulsed Dendritic Cell Vaccine
Fresh tumors were sent to the cell therapy laboratory. Tumor single-cell suspensions were obtained by mechanical disaggregation and then frozen and stored. Tumor lysate was obtained through four cycles of thawing and freezing, and then irradiated and stored at −20 °C. Seven days after dexamethasone termination, peripheral blood mononuclear cells were collected by leukapheresis. CD14+ cells were selected by immunomagnetic separation using a CliniMacs™ (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instructions. These cells were cultured at 2 × 106 cells/mL in AIM-V (Gibco, Grand Island NY 14072,USA) supplemented with antibiotics, 1000 UI/mL of IL-4 (R&D Systems, Minneapolis, MN, USA) and 1000 UI/mL GM-CSF (Leukine, Genzyme Corporation, Bayer Healthcare, Seattle, WA, USA) in culture bags (Cellgenix, Gaithersburg, MD 20877, USA) at 37 °C in a humidified incubator. IL-4 (500 UI/mL) and GM-CSF (500 UI/mL) were further added to the medium on the 4th day, and cultured cells were harvested on the 7th day. These immature dendritic cells were adjusted at 107 cells/mL and pulsed with autologous tumor lysate (median 69.82 μg/mL, rank 27.9–75 μg/mL) for 2 h at 37 °C and 5% CO2. At that time, to induce dendritic cells maturation, 50 ng/mL of TNF-α (Beromun, Boehringer Ingelheim, Barcelona, España), 1000 UI/mL of IFN-α (Intron A, Schering Corporation, Kenilworth, NJ, USA) and 20 ng/mL Poli I:C (Amersham, GE Healthcare, Madrid, España) were added to the medium and cells were placed in culture bags at 2 × 106 cells/mL. Mature dendritic cells were harvested on the 8th day and frozen in aliquots following standard procedures until use. Briefly, the cells were resuspended in RPMI-1640 complete medium (500 mL RPMI-1640 (GIBCO, Life Technology, Madrid, España) + 50 mL of 10% FCS + 5 mL of l-Glutamine 200 mM (GIBCO, Life Technology, Madrid, España) + 5 mL Pen/Strep solution (solution with 10,000 U/mL Pen, 10 mg/mL Strep, GIBCO, Life Technology) at twice the desired cryopreservation concentration. The cryopreservation solution was prepared, containing 40% complete RPMI-1640, 40% FCS and 20% DMSO. The cryopreservation vials were placed in the cryopreservation box (5100 Crio 1° Freezing Container, Nalgene) and 500 microliters of the cell suspension was added to each vial; then, 500 μL of the cryopreservation solution was added and the final suspension was carefully mixed. The cryopreservation box was brought to a −80 °C freezer, and after 24 h, the cell vials were stored in a liquid nitrogen tank. Ten million cells was considered the optimal dose for each administration. The viability of cells was determined before and after freezing [22 (link)].
Mejías Sosa L., López-Janeiro Á., Córdoba Iturriagagoitia A., Sala P., Solans B.P., Hato L., Inogés S., López-Díaz de Cerio A., Guillén-Grima F., Espinós J., De La Cruz S., Lozano M.D., Idoate M.A, & Santisteban M. (2023). Modification of Breast Cancer Milieu with Chemotherapy plus Dendritic Cell Vaccine: An Approach to Select Best Therapeutic Strategies. Biomedicines, 11(2), 238.
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Publication 2023
Antibiotics Cell Culture Techniques Cells Cell Survival Cell Therapy Cryopreservation Cultured Cells Dendritic Cells Dexamethasone Glutamine Granulocyte-Macrophage Colony-Stimulating Factor Immunomagnetic Separation Interferon-alpha Introns Leukapheresis Leukine Neoplasms Nitrogen PBMC Peripheral Blood Mononuclear Cells Poly A Streptococcal Infections Sulfoxide, Dimethyl Tumor Necrosis Factor-alpha
3
Evaluation of Parasitic Protozoa in Fecal and Water Samples
Fecal and stool samples were examined using a wet preparation method (WM) followed by the Sheather’s sugar flotation technique. This technique uses a sucrose solution with a specific gravity of 1.21 to concentrate the sample. Once the sample is concentrated, thin smears were made on glass slides. The slides were air-dried, methanol-fixed, stained with modified Ziehl–Neelsen (MZN) staining, and examined under a light microscope (40X, 100X).
To examine the water samples, 5 L of each sample was filtered using a 142-mm diameter membrane filter with a pore size of 1.2 μm using a vacuum pump. The filtered sample was centrifuged for 10 min at 3000 g. The sediment pellet containing oocysts was subjected to sucrose flotation and immunomagnetic separation (IMS) and PCR methods25 . One drop of this concentrate was smeared on a slide, stained using the modified Ziehl–Neelsen technique26 (link), and then examined under a microscope (40X, 100X). Microscopically positive samples were stored at − 20 °C for subsequent DNA extraction. The remaining microscopically positive samples were stored in refrigerator in order to evaluate antiprotozoal activity of nanoparticles.
Abou Elez R.M., Attia A.S., Tolba H.M., Anter R.G, & Elsohaby I. (2023). Molecular identification and antiprotozoal activity of silver nanoparticles on viability of Cryptosporidium parvum isolated from pigeons, pigeon fanciers and water. Scientific Reports, 13, 3109.
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Publication 2023
A-A-1 antibiotic Antiprotozoal Agents Carbohydrates Feces Immunomagnetic Separation Light Microscopy Methanol Microscopy Oocysts Sucrose Tissue, Membrane Vacuum
4
Isolation and Culture of Human T Cells
The study was approved by the local institutional review board of the Faculty of Medicine of the TU Dresden (EK138042014). Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats of healthy donors via density gradient centrifugation. Untouched CD3+ T cells were isolated from freshly prepared PBMCs using immunomagnetic separation according to the manufacturer’s instructions (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). The purity of the isolated cell population was > 90% as assessed by flow cytometric analysis. Isolated T cells were cultured in RPMI complete medium (Feldmann et al., 2011 (link)) supplemented with 50 U/ml interleukin (IL)-2 (Miltenyi Biotec GmbH).
Arndt C., Tunger A., Wehner R., Rothe R., Kourtellari E., Luttosch S., Hannemann K., Koristka S., Loureiro L.R., Feldmann A., Tonn T., Link T., Kuhlmann J.D., Wimberger P., Bachmann M.P, & Schmitz M. (2023). Palbociclib impairs the proliferative capacity of activated T cells while retaining their cytotoxic efficacy. Frontiers in Pharmacology, 14, 970457.
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Publication 2023
Centrifugation, Density Gradient Culture Media Donors Ethics Committees, Research Faculty Flow Cytometry Immunomagnetic Separation Interleukin-2 PBMC Peripheral Blood Mononuclear Cells Pharmaceutical Preparations T-Lymphocyte
5
Multiparametric Flow Cytometry of Cardiac Immune Cells
Peripheral blood (50 μL) was taken immediately after cervical dislocation for flow cytometric analysis. RBCs were lysed with 2 mL ACK lysing buffer (Gibco, Thermo Fisher Scientific, A10492-01) for 10 minutes. Excised LV infarcted tissue was rinsed and immediately minced and digested by warm digestion buffer containing collagenase type 2 (0.05%, Worthington Biochemical, LS004177) and DNase 1 (60 U/mL DNase 1, MilliporeSigma, 10104159001), followed by shaking for 5 minutes in 37°C. A single-cell suspension was generated and filtered through a 40 μm filter into ice-cold stopping buffer containing 10% FBS in 5 mL PBS. This process was repeated, with the tissue remaining in the filter for a total of 5 digestions. Cells were centrifuged (400g, 5 min), and RBCs were lysed with 2 mL ACK lysing buffer (Gibco, Thermo Fisher Scientific, A10492-01) for 5 minutes. Cells were then washed twice with PBS. Immune cells isolated from blood and infarcted heart tissue were resuspended in 100 μL staining buffer (1% PBS in 2 mM EDTA). The cells were incubated with 1% CD16/CD32 for 10 minutes at room temperature followed by incubation of a 1% antibody mixture on ice for 20 minutes. Cells were then washed twice with iced PBS, counted, and fixed. For flow cytometric analysis, data were acquired with an Aurora flow cytometer (Cytek) and analyzed with FlowJo 10.7.1 software (FlowJo). Viability stain 7-AAD was used to identify live cells. The gating strategies are shown in Supplemental Figure 6 (neutrophils: CD45+CD11b+ly6G+, monocytes: CD45+CD11b+ly6G–, ly6Chi monocytes: CD45+CD11b+ly6G–ly6Chi, and CD206+ macrophages: CD45+CD11b+ly6G–CD206+). Antibodies against CD45-BV421, CD11b-APC, Ly6G-APCcy7, Ly6C-AF700, and CD206-PE were used for flow cytometry (see Supplemental Table 2 for details). For cM cell separation, an immunomagnetic positive selection cell isolation kit was used (catalog 18970, EasySep).
Cai S., Zhao M., Zhou B., Yoshii A., Bugg D., Villet O., Sahu A., Olson G.S., Davis J, & Tian R. (2023). Mitochondrial dysfunction in macrophages promotes inflammation and suppresses repair after myocardial infarction. The Journal of Clinical Investigation, 133(4), e159498.
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Publication 2023
Antibodies BLOOD Buffers Cells Cell Separation Cold Temperature Deoxyribonucleases Digestion Edetic Acid Erythrocytes Flow Cytometry Heart Immunoglobulins Immunomagnetic Separation ITGAM protein, human Joint Dislocations Macrophage Monocytes Neck Neutrophil Neutrophil Collagenase Stains Tissues

Top products related to «Immunomagnetic Separation»

1
Magnetic activated cell sorting (macs) by Miltenyi Biotec
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MACS is a magnetic cell separation technology developed by Miltenyi Biotec. It enables the efficient isolation and enrichment of target cells from complex biological samples. The core function of MACS is to provide a gentle, reliable, and scalable method for cell separation and purification.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Cd34 microbead kit by Miltenyi Biotec
Sourced in Germany, United States, United Kingdom, Canada
The CD34 MicroBead Kit is a laboratory product that enables the separation and enrichment of CD34-positive cells from a variety of sample types, including bone marrow, peripheral blood, and leukapheresis samples. The kit utilizes magnetic beads coated with antibodies specific to the CD34 cell surface antigen to facilitate the isolation of the target cell population.
4
Immunomagnetic separation by Miltenyi Biotec
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Immunomagnetic separation is a laboratory technique used to isolate specific cell types or molecules from a heterogeneous sample. It utilizes magnetic beads coated with antibodies or ligands that bind to the target analyte. The magnetically labeled target can then be separated from the rest of the sample using a magnetic field.
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Easysep by STEMCELL
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EasySep is a magnetic cell separation system designed for the isolation of specific cell populations from complex samples. The system utilizes immunomagnetic particles that bind to target cells, allowing for their separation from the rest of the sample using a magnetic field. This enables the rapid and gentle isolation of desired cell types with high purity.
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Interleukin 4 (il 4) by Thermo Fisher Scientific
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IL-4 is a protein that plays a role in the regulation of immune responses. It is a component of laboratory equipment used for research and analysis in the field of immunology.
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The QIAamp DNA Mini Kit is a laboratory equipment product designed for the purification of genomic DNA from a variety of sample types. It utilizes a silica-membrane-based technology to efficiently capture and purify DNA, which can then be used for various downstream applications.
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Immunomagnetic separation system by BD
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The Immunomagnetic Separation System is a laboratory equipment designed for the isolation and purification of specific cell types, proteins, or other target molecules from complex biological samples. The system utilizes magnetic beads coated with antibodies or other affinity ligands to selectively bind and capture the desired target, which can then be separated from the rest of the sample using a magnetic field. The core function of this system is to provide a reliable and efficient method for the isolation and enrichment of target analytes for further analysis or downstream applications.
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Trizol reagent by Thermo Fisher Scientific
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
10
Immunomagnetic cell separation by Miltenyi Biotec
Sourced in Germany
Immunomagnetic cell separation is a laboratory technique used to isolate and purify specific cell types from a heterogeneous cell population. The core function of this technology is to selectively label target cells with magnetic particles, allowing their separation and collection using a magnetic field.

More about "Immunomagnetic Separation"

Immunomagnetic Separation (IMS) is a powerful biomedical technique that enables the isolation and purification of specific cell types, molecules, or pathogens from complex mixtures.
This process involves the use of magnetic beads coated with antibodies or ligands that bind to the target of interest, allowing for highly selective and efficient separation.
IMS is widely used in various applications, such as the isolation of circulating tumor cells, stem cells, or infectious agents.
It is also employed in diagnostics and therapeutic applications, making it an essential tool for advancing scientific discoveries and improving patient care.
The versatility of IMS lies in its ability to maintain the integrity of the separated targets while achieving high purity and recovery rates.
This is achieved through the use of magnetic fields, which allow the magnetically-labeled targets to be efficiently separated from the rest of the sample.
IMS can be combined with other techniques, such as MACS (Magnetic-Activated Cell Sorting), FBS (Fetal Bovine Serum), and CD34 MicroBead Kits, to further enhance the separation process and improve the overall quality of the isolated samples.
The EasySep™ and Immunomagnetic Separation System are examples of commercial IMS products that offer streamlined and efficient isolation of cells, proteins, and other biomolecules.
Additionally, the QIAamp DNA Mini Kit and TRIzol reagent can be used in conjunction with IMS to extract and purify nucleic acids from the separated targets.
Researchers can optimize their IMS protocols for reproducibility and accuracy by exploring the latest literature, pre-prints, and patents using platforms like PubCompare.ai.
This AI-driven tool helps researchers find the best protocols and products, ensuring their research is accurate and reliable.
By understanding the nuances of Immunomagnetic Separation and leveraging the latest advancements in the field, researchers can unlock the full potential of this powerful technique and drive scientific discoveries forward.