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Infrared Spectrophotometry
Infrared Spectrophotometry
Infrared Spectrophotometry is a powerful analytical technique used to identify and characterize chemical compounds by measuring the absorption of infrared radiation.
This method provides detailed information about the molecular structure and composition of samples, making it a valuable tool in fields such as chemistry, biochemistry, and materials science.
Infrared Spectrophotometry involves exposing a sample to infrared radiation and analyzing the resulting absorption spectrum, which is unique to the chemical bonds and functional groups present in the material.
This non-destructive technique can be applied to a wide range of solid, liquid, and gaseous samples, and is often used for qualitative and quantitative analysis, as well as for studying chemical reactions and monitoring industrial processes.
With its high sensitivity and reproducibilty, Infrared Spectrophotometry is an essential technique for researchers and scientists looking to unlock new insights and enhance the accuracy of their work.
This method provides detailed information about the molecular structure and composition of samples, making it a valuable tool in fields such as chemistry, biochemistry, and materials science.
Infrared Spectrophotometry involves exposing a sample to infrared radiation and analyzing the resulting absorption spectrum, which is unique to the chemical bonds and functional groups present in the material.
This non-destructive technique can be applied to a wide range of solid, liquid, and gaseous samples, and is often used for qualitative and quantitative analysis, as well as for studying chemical reactions and monitoring industrial processes.
With its high sensitivity and reproducibilty, Infrared Spectrophotometry is an essential technique for researchers and scientists looking to unlock new insights and enhance the accuracy of their work.
Most cited protocols related to «Infrared Spectrophotometry»
Curing Lights, Dental
Face
Infrared Spectrophotometry
Light
Polymerization
Radiotherapy
Spectroscopy, Fourier Transform Infrared
Transmission, Communicable Disease
zinc selenide
Unless otherwise stated, all chemical reagents purchased (Merck, Darmstadt, Germany or Aldrich, St. Louis, MO, USA) were of the highest commercially available purity and were used without previous purification. IR spectra were recorded as thin films in a FT-IR Nicolet 6700 spectrometer (Thermo Scientific, San Jose, CA, USA) and frequencies are reported in cm−1. 1H and 13C spectra were recorded on a Bruker Avance 400 Digital NMR spectrometer (Bruker, Rheinstetten, Germany), operating at 400.1 MHz for 1H and 100.6 MHz for 13C. Chemical shifts are reported in δ ppm and coupling constants (J) are given in Hz. HREIMS were measured on Thermo Finnigan MAT95XL mass spectrometers. Silica gel (Merck 200–300 mesh) was used for C.C. and silica gel plates HF 254 for TLC. TLC spots were detected by heating after spraying with 25% H2SO4 in H2O.
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Exanthema
Fingers
Infrared Spectrophotometry
Silica Gel
The nitrone 5 was prepared according to the literature protocols [15 (link)]. IR spectra were acquired on an FT-IR spectrometer in KBr and are reported in wave numbers (cm−1). Reactions were monitored by TLC carried out using UV light 254 nm, 1% aqueous permanganate and 10% solution of phosphomolibdic acid in ethanol and/or Dragendorff reagent as visualizing agents. Column chromatography was performed on silica gel 60 (70−230 mesh). 1H NMR spectra were recorded at 300, 400 or 500 MHz, and 13C NMR spectra were recorded at 75, 100 or 125 MHz. 1H and 13C chemical shifts (δ) were internally referenced to the residual solvent peak. For analysis and structure assignments of nitroxides, the samples were subjected to reduction with the zinc/CF3COOH system before recording of the NMR spectra as described below.
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1H NMR
Acids
Carbon-13 Magnetic Resonance Spectroscopy
Chromatography
Ethanol
Infrared Spectrophotometry
nitrones
nitroxyl
permanganate
Silica Gel
Solvents
Ultraviolet Rays
Zinc
Dentsply
Deuterium Oxide
Diamond
Esocidae
Infrared Spectrophotometry
Isotopes
Light
Light, Visible
mylar
Polymerization
Temporal Lobe
Escherichia coli JM109 cells (overnight culture in liquid nutrient medium Luria–Bertani (pH 7.2), 108 CFU) were washed twice with 0.01 M sterile PBS from the culture medium by centrifuging (Eppendorf centrifuge 5415C, 10 min, 12,000× g). Cell suspensions (107 CFU/mL) were incubated with antibiotic samples; then, after 1-2-12-24 h, the cell’s samples were suspended and aliquots of 0.5 mL were taken. The cells are precipitated by centrifugation and separated from the supernatant, washed twice, and resuspended in 50 µL PBS to register the IR spectra. The supernatant is separated to determine the amounts of unabsorbed substances. ATR-FTIR spectra of cells samples suspension were recorded using a Bruker Tensor 27 spectrometer equipped with a liquid nitrogen-cooled MCT (mercury cadmium telluride) detector. Samples were placed in a thermostatic cell BioATR-II with a ZnSe ATR element (Bruker, Bremen, Germany). The FTIR spectrometer was purged with a constant flow of dry air (Jun-Air, Michigan, USA). FTIR spectra were acquired from 900 to 3000 cm−1 with 1 cm−1 spectral resolution. For each spectrum, 50–70 scans were accumulated at a 20 kHz scanning speed and averaged. Spectral data were processed using the Bruker software system Opus 8.2.28 (Bruker, Bremen, Germany), which includes linear blank subtraction, baseline correction, differentiation (second order, 9 smoothing points), min-max normalization, and atmosphere compensation. When necessary, 11-point Savitsky–Golay smoothing was used to remove noise. Peaks were identified by the standard Bruker picking-peak procedure. The concentration of Rif inside the cells was calculated from the material balance considering the unabsorbed Rif by UV-vis spectroscopy.
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Antibiotics
Atmosphere
Cells
Centrifugation
Culture Media
Escherichia coli
Infrared Spectrophotometry
mercury cadmium telluride
Nitrogen
Nutrients
Place Cells
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis
Sterility, Reproductive
Most recents protocols related to «Infrared Spectrophotometry»
All chemicals and solvents were prepared purely from Merck & Aldrich. Reaction progress was examined by thin-layer chromatography on PolyGram SILG / UV254 plates. Melting points of the synthesized compounds were measured by a Buchi B-540 B device. FT-IR analysis was performed to identify the synthesized compounds by Bruker. The reported FT-IR spectra were taken by KBr tablets in the range of 400 to 4000. Proton and carbon nuclear magnetic resonance (1HNMR, 13CNMR) spectra were recorded in Bruker (DRX-400 Avance) and DMSO-d6 was used as the solvent. FE-SEM (MIRA3TESCAN-XMU) was used to evaluate and compare the surface of the composite and GO. SAMX MIRA II was used for EDS analysis. Composite crystallographic characterization was performed by X'Pert PRO MPD P decomposition using Ni-FILTERED filtered Cu-K rays in the diffraction angle range of 5–80. Thermal decomposition analysis was performed by STA 505 under argon atmosphere.
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Argon
Atmosphere
Carbon
Crystallography
Infrared Spectrophotometry
Magnetic Resonance Imaging
Medical Devices
Protons
Radiation
Solvents
Strains
Sulfoxide, Dimethyl
Thin Layer Chromatography
Sample solutions were directly infused
in a 3D quadrupole ion trap (QIT) mass spectrometer (Bruker, Amazon
Speed ETD, Bremen, Germany) coupled to the beamline of the free-electron
laser for Infrared eXperiments (FELIX),52 (link) as explained in theSupporting Information . During an IRMPD “action” spectroscopy experiment,
the absorption of multiple resonant IR photons leads to an increase
in the internal energy of the analyte, causing its unimolecular dissociation
and, consequently, the appearance of fragment ions in the mass spectrum.
To prevent excessive depletion of the parent ions and to minimize
the formation of fragment ions below the low mass cut of the QIT,
the IR spectra were recorded at several levels of laser pulse energy
attenuation.53 (link)
in a 3D quadrupole ion trap (QIT) mass spectrometer (Bruker, Amazon
Speed ETD, Bremen, Germany) coupled to the beamline of the free-electron
laser for Infrared eXperiments (FELIX),52 (link) as explained in the
the absorption of multiple resonant IR photons leads to an increase
in the internal energy of the analyte, causing its unimolecular dissociation
and, consequently, the appearance of fragment ions in the mass spectrum.
To prevent excessive depletion of the parent ions and to minimize
the formation of fragment ions below the low mass cut of the QIT,
the IR spectra were recorded at several levels of laser pulse energy
attenuation.53 (link)
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Infrared Spectrophotometry
Mass Spectrometry
Parent
Pulse Rate
Spectrum Analysis
The reagents and solvents were purchased from commercial suppliers and used without further purification unless indicated otherwise. Microwave-assisted reactions were conducted using a CEM Discover Synthesis Unit (CEM Corp., Matthews, NC, USA). The purification of the reaction mixtures was performed using flash chromatography on a glass column with silica gel (high-purity grade (9385), 60 Å, 230–400 mesh, Merck KGaA, Darmstadt, Germany). For thin layer chromatography, ALUGRAM® pre-coated TLC plates (Silica gel 60 F254, MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) were employed. Melting points were determined using DigiMelt MPA160 apparatus (Nyköping, Sweden) and are uncorrected. The IR spectra were recorded on a Brüker TENSOR 27 (Brüker Optik GmbH, Ettlingen, Germany) spectrometer using KBr pellets. NMR spectra were recorded using Brüker Avance III spectrometer (400 MHz for 1H NMR, 100 MHz for 13C NMR, 40 MHz for 15N NMR; Brüker BioSpin AG, Fallanden, Switzerland) at 25 °C. Residual solvent signals were used as internal standards, i.e., for DMSO-d6δ1H = 2.50 and δ13C = 39.52, for CDCl3δ1H = 7.26 and δ13C = 77.16, for acetone-d6δ1H = 2.05 and δ13C = 29.84. A neat external nitromethane standard was used to recalculate 15N chemical shifts. The full and unambiguous assignments of the 1H, 13C, 15N-NMR resonances were achieved using standard Brüker software and a combination of advanced NMR spectroscopic techniques. High-resolution mass spectra were recorded on a micrOTOF-Q III Brüker spectrometer (Brüker Daltonik GmbH, Bremen, Germany) in electrospray ionization mode.
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1H NMR
Acetone
Anabolism
Carbon-13 Magnetic Resonance Spectroscopy
Chromatography
Infrared Spectrophotometry
Mass Spectrometry
Microwaves
nitromethane
Pellets, Drug
Silica Gel
Solvents
Spectroscopy, Nuclear Magnetic Resonance
Sulfoxide, Dimethyl
Thin Layer Chromatography
Vibration
Na2CO3, NaCl, and 20% HCl were obtained from Fluka, while 4-nitrobenzene sulfonyl chloride and 4-hydroxyproline were obtained from Sigma Aldrich (Bristol Scientific Nigeria). IR spectra were recorded on Bruker FT-IR spectrophotometer. The NMR peaks were recorded on Bruker DPX 300 spectrophotometer with 1H at 400 MHz and 13 ℃ at 100 MHz. Chemical shifts δ are given in ppm and referenced to tetramethylsilane. Methanol was used as a solvent for crystallization. X-ray data of the single crystal were collected on an XtaLAB Synergy, Dualflex, Pilatus 200 K diffractometer using CuKα radiation at 105.4 (7)K. The pharmacokinetic properties were studied using SwissADME free online tool (https://www.swissadme.ch ), while the Molecular docking was carried out using the Molecular operating environment (MOE) (AutoDock Vina, BIOVIA).
Crystallization
Electromagnetic Radiation
Hydroxyproline
Infrared Spectrophotometry
Methanol
Nitrobenzenes
Radiography
Sodium Chloride
Solvents
sulfonyl chloride
tetramethylsilane
All used chemicals
and solvents are from Merck, Aldrich, and Fluke chemicals in this
work. The spectra of FT-IR were performed with pellets of KBr using
a Nexus 670 FT-IR spectrometer (Daypetronic Company, Tehran, Iran).
TL-chromatography was used to monitor the reaction over silica gel
plates. Particle morphology was investigated using a scanning electron
microscope (Daypetronic Company, Iran-Tehran) through FESEM-TESCAN
MIRA3. The 500 MHz spectra of 1H NMR and 13C
NMR were obtained using a spectrometer of BRUKER NMR (Daypetronic
Company, Iran-Tehran). Energy scattered X-rays of MNPS were
recorded using FESEM-TESCAN MIRA3 (Daypetronic Company, Tehran, Iran).
XRD was performed using Co radiation with a 40 kV wavelength scenting
P AD V X-ray (Beamgostar Taban Company, Tehran, Iran). The samples
were scanned in the ranges of 2θ = 1–10° and 2θ
= 10–80°; a BET isotherm of N2 was recorded
at 77 °K employing a standard gas manifold to explain the features
of materials like average pore diameter, pore-volume, and catalyst
surface (Daypetronic Company, Tehran, Iran). In addition, adsorption
data are presented in Brunauer–Emmett–Teller (BET) method.
In all reactions, CH3COOH with a molecular weight of 400
was applied. The content of Zn was measured by ICP-OES analysis (Tarbiat
Modares University, Tehran, Iran).
and solvents are from Merck, Aldrich, and Fluke chemicals in this
work. The spectra of FT-IR were performed with pellets of KBr using
a Nexus 670 FT-IR spectrometer (Daypetronic Company, Tehran, Iran).
TL-chromatography was used to monitor the reaction over silica gel
plates. Particle morphology was investigated using a scanning electron
microscope (Daypetronic Company, Iran-Tehran) through FESEM-TESCAN
MIRA3. The 500 MHz spectra of 1H NMR and 13C
NMR were obtained using a spectrometer of BRUKER NMR (Daypetronic
Company, Iran-Tehran). Energy scattered X-rays of MNPS were
recorded using FESEM-TESCAN MIRA3 (Daypetronic Company, Tehran, Iran).
XRD was performed using Co radiation with a 40 kV wavelength scenting
P AD V X-ray (Beamgostar Taban Company, Tehran, Iran). The samples
were scanned in the ranges of 2θ = 1–10° and 2θ
= 10–80°; a BET isotherm of N2 was recorded
at 77 °K employing a standard gas manifold to explain the features
of materials like average pore diameter, pore-volume, and catalyst
surface (Daypetronic Company, Tehran, Iran). In addition, adsorption
data are presented in Brunauer–Emmett–Teller (BET) method.
In all reactions, CH3COOH with a molecular weight of 400
was applied. The content of Zn was measured by ICP-OES analysis (Tarbiat
Modares University, Tehran, Iran).
1H NMR
Chromatography
Electromagnetic Radiation
Electron Microscopy
Infrared Spectrophotometry
Nexus
Pellets, Drug
Radiography
Silicon Dioxide
Solvents
Trematoda
Top products related to «Infrared Spectrophotometry»
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The Vertex 70 is a Fourier Transform Infrared (FTIR) spectrometer manufactured by Bruker. It is designed to perform high-resolution infrared spectroscopy analysis of various samples. The Vertex 70 provides accurate measurements of the absorption, emission, or reflectance properties of materials across the infrared spectrum.
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Silica gel is a porous, amorphous form of silicon dioxide. It is a desiccant material commonly used for moisture absorption and control in various applications.
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Silica gel 60 is a porous, amorphous form of silicon dioxide commonly used as a stationary phase in column chromatography. It has a high surface area and is effective at adsorbing a wide range of organic and inorganic compounds. Silica gel 60 is available in various particle sizes and pore sizes to suit different chromatographic applications.
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Silica gel 60 F254 is a type of silica gel thin-layer chromatography (TLC) plate. It is a planar solid support material used for the separation and identification of chemical compounds. The silica gel 60 F254 plate contains a fluorescent indicator that allows for the visualization of separated compounds under ultraviolet (UV) light.
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Sephadex LH-20 is a size-exclusion chromatography media developed by GE Healthcare for the separation and purification of a wide range of organic molecules, including proteins, peptides, nucleic acids, and small organic compounds. It is composed of cross-linked dextran beads and is designed for use in gravity-flow or low-pressure liquid chromatography applications.
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The Nicolet 6700 is a Fourier-transform infrared (FTIR) spectrometer designed for a wide range of analytical applications. It features high-performance optics and a sensitive detector to provide accurate and reliable spectroscopic data.
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The P-1020 polarimeter is a laboratory instrument used to measure the optical rotation of a sample. It determines the degree of rotation of plane-polarized light passing through the sample, which can be used to identify or quantify specific substances.
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The Tensor 27 is a Fourier Transform Infrared (FTIR) spectrometer produced by Bruker. It is a compact and versatile instrument designed for a wide range of applications in materials science, pharmaceuticals, and other research fields. The Tensor 27 provides high-quality infrared spectroscopic data for the identification and analysis of organic and inorganic compounds.
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Sephadex LH-20 is a size-exclusion chromatography medium used for the separation and purification of a wide range of molecules, including proteins, peptides, and small organic compounds. It is a hydrophilic, cross-linked dextran polymer with a porous structure that allows for size-based separation. Sephadex LH-20 is commonly used in various applications, such as desalting, fractionation, and purification of samples.
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The P-2000 polarimeter is a laboratory instrument used to measure the optical rotation of a sample. It determines the angle of rotation of the plane of polarized light passing through a sample, which can be used to analyze the chemical composition or concentration of chiral molecules.
More about "Infrared Spectrophotometry"
Infrared Spectroscopy (IR Spectroscopy) is a powerful analytical technique that utilizes the absorption of infrared radiation to identify and characterize chemical compounds.
This non-destructive method provides detailed insights into the molecular structure and composition of samples, making it an invaluable tool across fields like chemistry, biochemistry, and materials science.
The IR Spectrophotometry process involves exposing a sample to infrared light and analyzing the resulting absorption spectrum, which is unique to the chemical bonds and functional groups present in the material.
This high-sensitivity, reproducible technique can be applied to a wide range of solid, liquid, and gaseous samples, enabling qualitative and quantitative analysis, as well as the study of chemical reactions and industrial processes.
Researchers and scientists can leverage IR Spectroscopy to unlock new discoveries and enhance the accuracy of their work.
Specialized equipment like the Vertex 70, Tensor 27, and Nicolet 6700 IR spectrometers, along with Silica gel 60 and Sephadex LH-20 purification media, are often used in IR Spectrophotometry studies.
The P-1020 and P-2000 polarimeters are also relevant for related optical analysis techniques.
By understanding the power of IR Spectroscopy and incorporating related terminology, researchers can optimize their research workflows and uncover valuable insights with greater efficiency and precision.
This non-destructive method provides detailed insights into the molecular structure and composition of samples, making it an invaluable tool across fields like chemistry, biochemistry, and materials science.
The IR Spectrophotometry process involves exposing a sample to infrared light and analyzing the resulting absorption spectrum, which is unique to the chemical bonds and functional groups present in the material.
This high-sensitivity, reproducible technique can be applied to a wide range of solid, liquid, and gaseous samples, enabling qualitative and quantitative analysis, as well as the study of chemical reactions and industrial processes.
Researchers and scientists can leverage IR Spectroscopy to unlock new discoveries and enhance the accuracy of their work.
Specialized equipment like the Vertex 70, Tensor 27, and Nicolet 6700 IR spectrometers, along with Silica gel 60 and Sephadex LH-20 purification media, are often used in IR Spectrophotometry studies.
The P-1020 and P-2000 polarimeters are also relevant for related optical analysis techniques.
By understanding the power of IR Spectroscopy and incorporating related terminology, researchers can optimize their research workflows and uncover valuable insights with greater efficiency and precision.