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Limulus Test

The Limulus Test, also known as the Limulus Amebocyte Lysate (LAL) test, is a sensitive and widely used assay for the detection of bacterial endotoxins.
Derived from the blood cells of the horseshoe crab (Limulus polyphemus), the test leverages the lysate's ability to coagulate in the presence of endotoxins, providing a reliable means to assess the purity of pharmaceuticals, medical devices, and other products.
Researchers can leverage PubCompare.ai's AI-driven platform to streamline the identification of optimal Limulus Test protocols from literature, pre-prints, and patents, enabling seamiless comparisons to enhance the reproducibility and accuaracy of their research and achieve reliable results.

Most cited protocols related to «Limulus Test»

1
Hyaluronic Acid Complex Characterization and Application
Cited 8 times
High- (H-HA; MW 1400 ± kDa, Mw/Mn = 1.5, Intrinsic viscosity =21.5 dl/g) and low-molecular weight hyaluronic acid (L-HA: Mw = 90 ± 5 kDa, Mw/Mn = 1.7 ± 0.07 Intrinsic viscosity =2.4 dl/g) were kindly provided by Altergon s.r.l., Italy. Altergon HA is a fermentative HA from Streptococcus equi ssp. equi, at pharmaceutical grade (e.g. purity >95 %, water content < 10 %, EU/mg < 0.05). This ensured that the products are controlled according to the pharmacopeia. Briefly in collaboration with Altergon, LHA 90 kDa was obtained through heterogeneous hydrolytic reaction in acidic conditions. The hydrodynamic characterization was performed using the SEC-TDA (Size Exclusion Chromatography-Triple Detector Array) equipment by Viscotek (Lab Service Analytica, Italy).
The H-HA/L-HA complex (1:1 weight ratio) was produced in our laboratory following the procedure described in patent application WO2011EP65633 [27 ], which was modified to obtain solutions (32 g/l) suitable for use in cell cultures (i.e., use of a physiological buffer solution, PBS). For all samples, pH and osmolality were measured in order to perform experiments in physiological conditions (i.e., pH 7.0–7.4; osmolality 300 mOsm). Endotoxin concentration (EU/mg) was evaluated with the Limulus test, and solutions were used only when the titer as <1 EU/mg. All solutions were sterilized in an autoclave at 1 bar for 12 min at 120 °C.
Bovine testicular hyaluronidase, BTH (EC 3.2.1.35), essentially salt free lyophilized powder with a specific activity of 1160U/mg was purchased from Sigma-Aldrich (Milan, Italy) (cat. N. H3884, lot. N. 057K7014).
HaCaT cells (Istituto Zooprofilattico, Brescia, Italy), a spontaneously transformed non-tumorigenic human keratinocyte cell line and human dermal fibroblast (HDF), a generous gift of Prof Caraglia, were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 % (v/v) heat inactivated fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/ml streptomycin. All materials were purchased from Flow Laboratories (Milan, Italy). The cells were grown on tissue culture plates (Corning Incorporated, New York, USA), using an incubator with a humidified atmosphere (95 % air/5%CO2 v/v) at 37 °C. Collagen was purchased from Sigma, Aldrich (Milan, Italy).
D’Agostino A., Stellavato A., Busico T., Papa A., Tirino V., Papaccio G., La Gatta A., De Rosa M, & Schiraldi C. (2015). In vitro analysis of the effects on wound healing of high- and low-molecular weight chains of hyaluronan and their hybrid H-HA/L-HA complexes. BMC Cell Biology, 16, 19.
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Publication 2015
Acids Atmosphere Bos taurus Buffers Cell Culture Techniques Cell Lines Cells Collagen Culture Media Eagle Endotoxins Fermentation Fetal Bovine Serum Fibroblasts Gel Chromatography Genetic Heterogeneity HaCaT Cells Hyaluronic acid Hyaluronidase Hydrodynamics Hydrolysis Keratinocyte Limulus Test Neoplastic Cell Transformation Penicillins Pharmaceutical Preparations physiology Powder Skin Sodium Chloride Streptococcus equi Streptomycin Testis Tissues Viscosity
2
Isolation and Characterization of Extracellular Vesicles
Cited 6 times
MVs were obtained from supernatants of MSCs and of HLSCs as previously described [8] (link), [10] . Briefly, MSCs were cultured in RPMI deprived of fetal calf serum (FCS) and supplemented with 0.5% of bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO). HLSCs were cultured overnight in α-MEM deprived of FCS. The viability of both cell types at the time of MV collection was >99% as detected by trypan blue exclusion. After centrifugation at 2,000 g for 20 minutes to remove debris, cell-free supernatants were ultracentrifuged at 100,000 g (Beckman Coulter Optima L-90K) for 1 hour at 4°C, washed in serum-free medium 199 containing Hepes 25 mM (Sigma) and submitted to a second ultracentrifugation in the same conditions [9] (link). The protein content of MV preparations was quantified by Bradford method (Bio-Rad, Hercules, CA, USA). In selected experiments, MVs were labeled with the red fluorescence aliphatic chromophore intercalating into lipid bilayers, PKH26 dye (Sigma). After labeling, MVs were washed and ultracentrifuged at 100,000 g for 1 h at 4°C. Endotoxin contamination of MVs was excluded by Limulus test (Charles River Laboratories, Inc., Wilmington, MA, USA) according to the manufacturer's instruction, and MVs were stored at –80°C. The analyses on MV suspension after staining with propidium iodide did not show the presence of apoptotic bodies.
Collino F., Deregibus M.C., Bruno S., Sterpone L., Aghemo G., Viltono L., Tetta C, & Camussi G. (2010). Microvesicles Derived from Adult Human Bone Marrow and Tissue Specific Mesenchymal Stem Cells Shuttle Selected Pattern of miRNAs. PLoS ONE, 5(7), e11803.
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Publication 2010
Apoptotic Bodies Cells Cell Survival Centrifugation Endotoxins Fetal Bovine Serum Fluorescence HEPES Limulus Test Lipid Bilayers PKH 26 Propidium Iodide Proteins Rivers Serum Serum Albumin, Bovine Trypan Blue Ultracentrifugation
3
Isolation and Characterization of Microvesicles
Cited 4 times
MVs were obtained from supernatants of hWJMSCs and of HFFs as previously described [30] (link), [31] (link). In brief, hWJMSCs were cultured in DMEM deprived of FBS and supplemented with 0.5% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO) overnight. HFFs were cultured in serum-free RPMI-1640 supplemented with 0.5% BSA. The viability of both cell types cultured in respective medium overnight was > 99% for hWJMSCs and 90% for HFFs as detected by trypan blue exclusion. No apoptotic cells were detected by TUNEL assay in hWJMSCs and < 3% apoptotic cells were detected for HFFs. After centrifugation at 2,000 g for 20 minutes to remove debris, cell-free supernatants were ultracentrifuged at 100,000 g in a SW41 swing rotor (Beckman Coulter Optima L-80K ultracentrifuge; Beckman Coulter, Fullerton, CA) for 1h at 4°C. MVs were washed once with serum-free M199 (Sigma-Aldrich) containing 25 mM HEPES (PH 7.4) and submitted to a second ultracentrifugation in the same conditions. To quantify the protein content, MV pellets were suspended in serum-free M199 and estimated by a Bradford assay (Bradford protein assay kit, P0006, beyotime institute of biotechnology). Endotoxin contaminations of MVs were excluded by Limulus test according to the manufacturer’s instruction (Charles River Laboratories, Inc., Wilmington, MA, USA) and MVs were stored at –80°C.
Wu S., Ju G.Q., Du T., Zhu Y.J, & Liu G.H. (2013). Microvesicles Derived from Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cells Attenuate Bladder Tumor Cell Growth In Vitro and In Vivo. PLoS ONE, 8(4), e61366.
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Publication 2013
Apoptosis Biological Assay Cells Cell Survival Centrifugation Culture Media Endotoxins HEPES In Situ Nick-End Labeling Limulus Test Pellets, Drug Proteins Rivers Serum Serum Albumin, Bovine Trypan Blue Ultracentrifugation
4
Production and Purification of AAV-9 Vectors
Cited 4 times
AAV-9 vectors (AV.RSV.AP) were produced using a previously reported triple plasmid transfection protocol.9 (link),10 (link) The cis-plasmid (pcisRSV.AP) for AAV production was published previously.32 (link) The AAV-9 packaging plasmid pRep2/Cap9 was a gift from Dr James Wilson at the University of Pennsylvania (Philadelphia, PA).33 (link),34 (link) Recombinant viral stocks were purified through three rounds of isopycnic CsCl ultracentrifugation followed by three changes of HEPES buffer at 4 °C for 48 hours. Viral titer and quality control were performed according to our previously published protocol.35 (link) Endotoxin contamination was examined using the limulus amebocyte lysate assay using the Endosafe limulus amebocyte lysate gel clot test kit (Charles Rivers Laboratories, Wilmington, MA). The endotoxin levels in our viral stocks were within the acceptable level recommended by the Food and Drug Administration.
Yue Y., Ghosh A., Long C., Bostick B., Smith B.F., Kornegay J.N, & Duan D. (2008). A Single Intravenous Injection of Adeno-associated Virus Serotype-9 Leads to Whole Body Skeletal Muscle Transduction in Dogs. Molecular therapy : the journal of the American Society of Gene Therapy, 16(12), 1944-1952.
Publication 2008
Biological Assay Buffers cesium chloride Cloning Vectors Clotrimazole endotoxin binding proteins Endotoxins HEPES Limulus Limulus Test Plasmids Rivers Transfection Ultracentrifugation
5
Schistosoma mansoni Cercariae Preparation
Cited 4 times
Cercariae of Schistosoma mansoni were shed from Biomphalaria glabrata snails harbouring patent infections, and following concentration by sedimentation on ice for 1 h were washed three times with sterile water. Cercariae were then centrifuged (1000 × g, 5 min) and the larval pellet was frozen at −20°C. Alternatively, cercariae were mechanically transformed using the method described by Ramalho-Pinto et al. (19 (link)) to generate larvae for subsequent in vitro culture. In brief, cercariae were mechanically transformed by vortexing for 90 s in RPMI-1640 containing 200 U ml-1 penicillin and 100 μg ml-1 streptomycin (Invitrogen, Paisley, UK) (RPMI-0) and cultured in vitro in RPMI-0 for 3 h at 37°C and 5% CO2 in a humidified incubator (20 (link)). The culture supernatant was then removed and the remaining larvae were washed to recover further released material. Pooled supernatants were concentrated 50-fold using centrifugal filter units (Ultrafree-MC with 5-kDa cut-off; Millipore, Watford, UK). As a control, an equivalent volume of RPMI-0 medium containing no parasite material was concentrated using the same method. The 3-h larval heads were isolated from their tails by centrifugation on a 40/70% discontinous Percoll gradient (21 (link)), washed seven times in RPMI-0 and then frozen or cultured for a further 18 h in M169 media (20 (link)) supplemented with 200 U ml-1 penicillin, 100 μg ml-1 streptomycin and 5% heat-inactivated low-endotoxin FCS (Harlan Seralab). After the requisite culture period, the larvae were washed four times with RPMI-0 to remove all traces of serum proteins and then frozen at −20°C.
The thawed larvae of different maturation stages were sonicated (21 kHz at 6.5 μm amplitude) for 3 min and then centrifuged (100 000 × g) for 1 h to yield a soluble cercarial preparation (SCP), and larvae at 3 h [3-h soluble schistosomula preparation (3hSSP)] and 18 h [18-h soluble schistosomula preparation (18hSSP)]. The concentrated supernatant released by larvae between 0 and 3 h (0-3hRP) and the RPMI-0 control (RPMIc) were treated in a similar way. Protein and endotoxin concentrations of the preparations were determined using the Coomasie Plus-200 assay (Perbio Science UK Ltd, Tattenhall, UK) and Pyrogent Plus® limulus amoebocyte lysate test kit (BioWhittaker, Wokingham, UK), respectively.
Jenkins S.J., Hewitson J.P., Ferret-Bernard S, & Mountford A.P. (2005). Schistosome larvae stimulate macrophage cytokine production through TLR4-dependent and -independent pathways. International immunology, 17(11), 1409-1418.
Publication 2005
5-hydroxyethoxy-N-acetyltryptamine Australorbis glabratus Biological Assay Centrifugation Cercaria endotoxin binding proteins Endotoxins Freezing Head Infection Larva Limulus Test Parasites Penicillins Percoll Preparation H Proteins Schistosoma mansoni Serum Proteins Snails Sterility, Reproductive Streptomycin Tail

Most recents protocols related to «Limulus Test»

1
Plasma Endotoxin Quantification via LAL Assay
Quantification of plasma endotoxin levels was obtained by the colorimetric limulus amebocyte lysate test (LAL QCL-1000TM, Lonza, Belgium), according to the manufacturer’s instructions. Absorbance was measured spectrophotometrically by FLUOstar Omega (BMG Labtech, Offenburg, Germany) at 410 nm. Corrections were made by the subtraction of the absorbance of the sample without the addition of LAL.
Clarysse M., Accarie A., Farré R., Canovai E., Monbaliu D., Gunst J., De Hertogh G., Vanuytsel T., Pirenne J, & Ceulemans L.J. (2023). Protective Effect of Oxygen and Isoflurane in Rodent Model of Intestinal Ischemia-Reperfusion Injury. International Journal of Molecular Sciences, 24(3), 2587.
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Publication 2023
Colorimetry endotoxin binding proteins Endotoxins Limulus Test Plasma
2
Characterization of Mesoporous Silica Rods
All MSR materials were analyzed before in vitro T-cell activation. Thermogravimetric analysis (TGA) was conducted to confirm removal of contaminants and the individual MSRs were processed for sizing via bright field microscopy (EVOS FL Cell Imaging System) and scanning electron microscopy (SEM) to determine rod dimensions and ultrastructure. Nitrogen physisorption (3Flex) was performed to determine the pore size, volume, and surface area of the MSRs. These analyses are summarized in Supplementary Table 2. MSRs were also evaluated for their ability to coat liposomes and release IL-2, relevant biological proxies to confirm consistent physical structure between batches. MSRs were further confirmed to be endotoxin free via the Limulus Amebocyte Lysate test (Charles River).
Zhang D.K., Adu-Berchie K., Iyer S., Liu Y., Cieri N., Brockman J.M., Neuberg D., Wu C.J, & Mooney D.J. (2023). Enhancing CAR-T cell functionality in a patient-specific manner. Nature Communications, 14, 506.
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Publication 2023
Biopharmaceuticals Cells endotoxin binding proteins Endotoxins Limulus Test Liposomes Microscopy MTRR protein, human Nitrogen Physical Examination Rivers Scanning Electron Microscopy T-Lymphocyte
3
Radiolabeling of GRPR-Targeting Peptide AMTG
A detailed description of the precursor synthesis is provided in the supplemental materials (available at http://jnm.snmjournals.org). Purification was accomplished via reversed-phase high-performance liquid chromatography (HPLC).
64Cu and 68Ga labeling was performed in analogy to an established protocol for 177Lu labeling (19 (link)). A detailed description of the labeling procedures is provided in the supplemental materials. [64Cu]CuCl2 was purchased from DSD-Pharma GmbH. [68Ga]GaCl3 was provided by ITM Isotope Technologies Munich SE. The radiolabeled reference, 3 -[125I]I-Tyr6-MJ9 (Supplemental Fig. 1), was prepared according to reported procedures (19 (link),21 (link)). Characterization of all GRPR ligands is provided in Supplemental Figures 2–4.
The synthesis of [68Ga]Ga-AMTG according to good manufacturing practices for human PET/CT studies was performed using a good radiopharmaceutical practice module (Scintomics GmbH) while using an SC-01 gallium peptide labeling kit (ABX). [68Ga]GaCl3 was obtained from a GalliaPharm generator (Eckert & Ziegler) and was trapped on a PS-H+ cartridge (ABX), which was eluted by a sodium chloride solution. The eluate was transferred in the reactor containing the AMTG precursor and the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer. The solution was heated and afterward transferred to a Sep-Pak C18 Light cartridge (Waters) for purification. After washing with water, the cartridge was eluted with ethanol and the solution was diluted with phosphate-buffered saline. The Cathivex-GV (Merck KGaA) was used as a sterile filter after the synthesis. Quality control included an instant thin-layer chromatography silica gel scan (NH4OAc/MeOH; Agilent), as well as an HPLC measurement against the corresponding reference compound, [natGa]Ga-AMTG. Compliance with the HEPES limit was determined by a spot test. Furthermore, a sterile filter integrity test, a limulus amebocyte lysate test, and a postapplication sterility test were performed. The ethanol concentration was measured by gas chromatography analysis.
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Publication 2023
Acids Anabolism cupric chloride endotoxin binding proteins Ethanol Gallium Gas Chromatography HEPES High-Performance Liquid Chromatographies Homo sapiens Isotopes Ligands Light Limulus Test Lutetium-177 Peptides Phosphates Radionuclide Imaging Radiopharmaceuticals Saline Solution Scan, CT PET Sep-Pak C18 Silica Gel Sterility, Reproductive Thin Layer Chromatography
4
Helicobacter pylori Components Extraction and Analysis
Helicobacter pylori reference strain CCUG 17874 (Culture Collection, University of Gothenburg, Gothenburg, Sweden), positive for vacuolating cytotoxin A (VacA) and cytotoxin associated gene A (CagA) protein was cultured under microaerophilic conditions according to the previously described procedure5 (link),12 (link). H. pylori reference strain as well as Mycobacterium bovis onco-BCG preparation (SYNTHAVERSE, Lublin, Poland), Brazilian Moreau substrain, were used in the cell study in vitro in the multiplicity of infection (MOI): 10:1. Bacterial surface components from the reference H. pylori strain were extracted using 0.2 M glycine buffer, pH 2.2, as previously described54 (link). The glycine acid extract (GE) was evaluated for protein composition by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and a Western blotting (immunoblotting assay) (Milenia Blot H. pylori, DPC Biermann, GmbH, Bad Nanheim, Germany). For this purpose the reference serum samples from patients infected with H. pylori were used. Major proteins in GE recognized by the sera of patients are: 120 kDa, 80 kDa, between 66 and 42 kDa and 29–26 kDa. The protein content in GE was 600 μg/mL (NanoDrop 2000c Spectrophotometer, Thermo Fischer Scientific, Waltham, MA, USA). The GE sample contained < 0.001 EU mL of LPS as shown by the chromogenic Limulus amebocyte lysate test (Lonza, Walkersville, MD, USA). LPS from the reference H. pylori strain (obtained by courtesy of AP. Moran), was prepared by hot phenol-water extraction after pre-treatment of the bacterial biomass with protease. The crude LPS preparation was purified by RNAse, DNAse and proteinase K treatment and by ultracentrifugation, as previously described55 (link). E. coli LPS derived from the O55:B5 strain (Sigma Aldrich, Saint Louis, MO, USA), was used as control. The concentration of H. pylori components was selected experimentally or used as previously described, and was equal to: GE 10 µg/mL, H. pylori LPS and E.coli LPS 25 ng/mL4 (link),5 (link),12 (link).
Gonciarz W., Chyb M, & Chmiela M. (2023). Diminishing of Helicobacter pylori adhesion to Cavia porcellus gastric epithelial cells by BCG vaccine mycobacteria. Scientific Reports, 13, 16305.
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Publication 2023
Acids Aftercare azo rubin S Bacteria Buffers Calmette-Guerin Bacillus Cells Cytotoxin Deoxyribonucleases Endopeptidase K endotoxin binding proteins Escherichia coli Genes Glycine Helicobacter pylori Hydrogen Immunoblotting Infection Limulus Test lipopolysaccharide, Helicobacter pylori Patients Peptide Hydrolases Phenols Proteins Pylorus Ribonucleases SDS-PAGE Serum Strains Ultracentrifugation
5
Isolation and Stimulation of Primary Rat Glial Cells
Primary rat mixed glial cultures containing microglia and astrocytes were isolated as previously described (11 (link)). Following the 10-d cultivation period, microglia were collected from flask supernatants that were shaken at 180 rpm/min at 37 °C. Subsequently, astrocytes and microglia were purified using MACS according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch-Gladbach, Germany). Briefly, the remaining astrocytes were dislodged by trypsin-ethylenediaminetetraacetic acid-treatment (Thermo Fisher Scientific), labeled using a combination of anti-ACSA1-biotinylated antibodies in combination with antibiotin microbeads (Miltenyi Biotec) and applied to the isolation column. Microglial cells were detached by accutase treatment and labeled with anti-rat CD11b/c microbeads (Miltenyi Biotec). Cells were assessed for viability and counted under trypan blue staining. Cell purities as determined via Gfap/Iba1-staining were consistently >98%. Astrocytes (30,000 cells/well, 2 wells/ condition) and microglia (100,000 cells/transwell, 2 wells/condition) were seeded on 24-well plates and respective transwells in DMEM medium containing 10% FCS, 2 mM L-glutamine and 50 U/mL penicillin/streptomycin (Thermo Fisher Scientific). After 24 h, cell cultures were stimulated with either 1 µg/mL recombinant HERV-W ENV protein (Protein’eXpert, Grenoble, France) or reconstitution buffer. To avoid side effects through the recombinant production of HERV-W ENV protein, Endotoxin levels were measured using the limulus amebocyte lysate-test and found to be below the detection limit (<5EU = mL).
Gruchot J., Lewen I., Dietrich M., Reiche L., Sindi M., Hecker C., Herrero F., Charvet B., Weber-Stadlbauer U., Hartung H.P., Albrecht P., Perron H., Meyer U, & Küry P. (2023). Transgenic expression of the HERV-W envelope protein leads to polarized glial cell populations and a neurodegenerative environment. Proceedings of the National Academy of Sciences of the United States of America, 120(38), e2308187120.
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Publication 2023
accutase Anti-Antibodies Astrocytes ATP8A2 protein, human Buffers Cell Culture Techniques Cells Edetic Acid endotoxin binding proteins Endotoxins Glial Fibrillary Acidic Protein Glutamine Human Endogenous Retroviruses isolation ITGAM protein, human Limulus Test MAC combination Microglia Microspheres Neuroglia Penicillins Proteins Recombinant Proteins Streptomycin Trypan Blue Trypsin

Top products related to «Limulus Test»

1
Limulus amebocyte lysate test by Lonza
Sourced in United States, Belgium
The Limulus amebocyte lysate (LAL) test is a laboratory assay used to detect and quantify the presence of bacterial endotoxins. It is derived from the blood cells (amebocytes) of the horseshoe crab, Limulus polyphemus. The LAL test is a sensitive and reliable method for the detection of endotoxins, which are components of the cell walls of Gram-negative bacteria.
2
Limulus amebocyte lysate test by Associates of Cape Cod
Sourced in United States
Limulus amebocyte lysate (LAL) test is a laboratory assay used to detect and quantify bacterial endotoxin or lipopolysaccharide (LPS). It utilizes the clotting reaction of the amebocyte cells from the horseshoe crab (Limulus polyphemus) to indicate the presence of endotoxin in a sample.
3
Limulus color ky test by Fujifilm
Sourced in Japan
The Limulus Color KY Test is a laboratory equipment product that is used to detect the presence of endotoxins, a type of bacterial toxin, in various samples. The test utilizes the Limulus Amebocyte Lysate (LAL) reaction to identify endotoxin contamination. The product provides a simple and efficient method for analyzing the presence of endotoxins in a wide range of materials.
4
Limulus amebocyte lysate test by Cambrex
Sourced in United States
The Limulus amebocyte lysate (LAL) test is a laboratory technique used to detect the presence of endotoxins, a type of bacterial toxin. The test utilizes the blood cells of the horseshoe crab to indicate the presence of these toxins. It is a sensitive and reliable method for detecting endotoxin contamination in various samples, such as pharmaceutical products, medical devices, and water systems.
5
Limulus color ky test wako by Fujifilm
Sourced in Japan
The Limulus Color KY Test Wako is a laboratory equipment product designed for endotoxin detection. It is a chromogenic kinetic LAL (Limulus Amebocyte Lysate) test that measures the presence and concentration of endotoxins in samples.
6
Lipopolysaccharides (lps) by Merck Group
Sourced in United States, Germany, China, United Kingdom, Sao Tome and Principe, Macao, Italy, Japan, Canada, France, Switzerland, Israel, Australia, Spain, India, Ireland, Brazil, Poland, Netherlands, Sweden, Denmark, Hungary, Austria, Mongolia
The LPS laboratory equipment is a high-precision device used for various applications in scientific research and laboratory settings. It is designed to accurately measure and monitor specific parameters essential for various experimental procedures. The core function of the LPS is to provide reliable and consistent data collection, ensuring the integrity of research results. No further details or interpretations can be provided while maintaining an unbiased and factual approach.
7
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
8
Kinetic qcl endotoxin kit by Lonza
Sourced in United States
The Kinetic-QCL endotoxin kit is a laboratory equipment product designed for the detection and quantification of endotoxins. It utilizes a kinetic chromogenic assay method to measure the presence of endotoxins in samples.
9
Endosafe pts by Charles River Laboratories
Sourced in United States
The Endosafe PTS is a rapid test system for the detection and quantification of bacterial endotoxin. It provides accurate and reliable results in a short timeframe, enabling efficient quality control processes.
10
Model 680 by Bio-Rad
Sourced in United States, Japan, United Kingdom, China, Canada
The Model 680 is a microplate reader designed for absorbance-based assays. It can measure absorbance at multiple wavelengths and is suitable for a variety of microplate formats. The core function of the Model 680 is to quantify the absorbance of samples in a microplate, providing data for further analysis.

More about "Limulus Test"

The Limulus Test, also known as the Limulus Amebocyte Lysate (LAL) test, is a highly sensitive and widely utilized assay for the detection of bacterial endotoxins (lipopolysaccharides or LPS).
Derived from the blood cells of the horseshoe crab (Limulus polyphemus), the LAL test leverages the lysate's ability to coagulate in the presence of endotoxins, providing a reliable means to assess the purity of pharmaceuticals, medical devices, and other products.
Researchers can leverage PubCompare.ai's AI-driven platform to streamline the identification of optimal Limulus Test and Limulus Amebocyte Lysate (LAL) test protocols from literature, pre-prints, and patents, enabling seamless comparisons to enhance the reproducibility and accuracy of their research and achieve reliable results.
The Limulus Color KY Test and Limulus Color KY Test Wako are variations of the Limulus Test that utilize colorimetric detection methods.
Additionally, the Kinetic-QCL endotoxin kit and Endosafe PTS systems are popular tools used in conjunction with the Limulus Test to quantify endotoxin levels, while the Model 680 instrument can be employed for automated Limulus Test analysis.
By incorporating these related technologies and techniques, researchers can further optimize their endotoxin detection and measurement processes, leading to more accurate and reproducible findings in their studies.