Sampling selection for NHANES III was based on a complex multistage area probability design. Children younger than 5 years, adults ≥60 years of age, non-Hispanic blacks, and Mexican Americans were oversampled to allow an adequate number of sample persons in these demographic groups from which population-based estimates could be derived. However, urine samples were not collected for children < 6 years of age. Data were collected through a household interview, and a standardized physical examination was conducted in a mobile examination center. Urine specimens for analyses, including those for measuring creatinine concentrations, were collected during this examination throughout the day. Pre-examination procedures depended on the age and health status of the individual. For example, persons > 12 years of age were asked to fast for 2–12 hr, depending on appointment times, and persons with known diabetes or < 12 years of age were asked to eat a normal diet before the examination. Sociodemographic information and medical histories of the survey participants and their families were collected during the household interviews. Details of the sample design have been published (Ezzati et al. 1992 ). The data set used in our analysis is a part of the public release data set for NHANES III (NCHS 2004a ).
Urinalysis
This process involves the physical, chemical, and microscopic analysis of urine samples to detect and monitor various health conditions, such as kidney disorders, urinary tract infections, and metabolic imbalances.
Urinalysis provides valuable insights into the body's overall well-being, helping clinicians make informed decisions about patient care.
By examining the color, clarity, pH, and specific gravity of urine, as well as the presence of various substances, urinalysis can help identify potential issues and guide appropriate treatment strategies.
This essential laboratory test is a vital component of comprehensive healthcare, contributing to the early detection and management of a wide range of medical conditions.
Most cited protocols related to «Urinalysis»
Sampling selection for NHANES III was based on a complex multistage area probability design. Children younger than 5 years, adults ≥60 years of age, non-Hispanic blacks, and Mexican Americans were oversampled to allow an adequate number of sample persons in these demographic groups from which population-based estimates could be derived. However, urine samples were not collected for children < 6 years of age. Data were collected through a household interview, and a standardized physical examination was conducted in a mobile examination center. Urine specimens for analyses, including those for measuring creatinine concentrations, were collected during this examination throughout the day. Pre-examination procedures depended on the age and health status of the individual. For example, persons > 12 years of age were asked to fast for 2–12 hr, depending on appointment times, and persons with known diabetes or < 12 years of age were asked to eat a normal diet before the examination. Sociodemographic information and medical histories of the survey participants and their families were collected during the household interviews. Details of the sample design have been published (Ezzati et al. 1992 ). The data set used in our analysis is a part of the public release data set for NHANES III (NCHS 2004a ).
All of ≥40 years old Koreans and their families are requested to have a biannual health check without cost [11 (link)]. Because all Korean citizens are registered with a 13-digit resident registration number for lifelong, the thorough population statistics can be calculated in this study. All Koreans have to register in the NHIS. The 13-digit resident registration number has to be used in all Korean hospitals and clinics. Thus, the medical records was prevented to be overlapped, even in case of a patient moves from one place to another. In addition, the Korean Health Insurance Review and Assessment (HIRA) system managed all medical treatments in Korea. The causes and date of death diagnosed by medical doctors on the death certificate are legally announced to administrative entity.
This NHIS included health insurance claim codes (procedures and prescriptions), diagnostic codes using the International Classification of Disease-10 (ICD-10), death records, socioeconomic data and health check-up data (body mass index [BMI], drinking, smoking habit, blood pressure, urinalysis, hemoglobin, fasting glucose, lipid parameters, creatinine, and liver enzymes) for each participant over the period from 2002 to 2013 [10 ,11 (link)].
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Most recents protocols related to «Urinalysis»
Example 8
Characterization of Absorption, Distribution, Metabolism, and Excretion of Oral [14C]Vorasidenib with Concomitant Intravenous Microdose Administration of [13C315N3]Vorasidenib in Humans
Metabolite profiling and identification of vorasidenib (AG-881) was performed in plasma, urine, and fecal samples collected from five healthy subjects after a single 50-mg (100 μCi) oral dose of [14C]AG-881 and concomitant intravenous microdose of [13C3 15N3]AG-881.
Plasma samples collected at selected time points from 0 through 336 hour postdose were pooled across subjects to generate 0—to 72 and 96-336-hour area under the concentration-time curve (AUC)-representative samples. Urine and feces samples were pooled by subject to generate individual urine and fecal pools. Plasma, urine, and feces samples were extracted, as appropriate, the extracts were profiled using high performance liquid chromatography (HPLC), and metabolites were identified by liquid chromatography-mass spectrometry (LC-MS and/or LC-MS/MS) analysis and by comparison of retention time with reference standards, when available.
Due to low radioactivity in samples, plasma metabolite profiling was performed by using accelerator mass spectrometry (AMS). In plasma, AG-881 was accounted for 66.24 and 29.47% of the total radioactivity in the pooled AUC0-72 h and AUC96-336 h plasma, respectively. The most abundant radioactive peak (P7; M458) represented 0.10 and 43.92% of total radioactivity for pooled AUC0-72 and AUC96-336 h plasma, respectively. All other radioactive peaks accounted for less than 6% of the total plasma radioactivity and were not identified.
The majority of the radioactivity recovered in feces was associated with unchanged AG-881 (55.5% of the dose), while no AG-881 was detected in urine. In comparison, metabolites in excreta accounted for approximately 18% of dose in feces and for approximately 4% of dose in urine. M515, M460-1, M499, M516/M460-2, and M472/M476 were the most abundant metabolites in feces, and each accounted for approximately 2 to 5% of the radioactive dose, while M266 was the most abundant metabolite identified in urine and accounted for a mean of 2.54% of the dose. The remaining radioactive components in urine and feces each accounted for <1% of the dose.
Overall, the data presented indicate [14C]AG-881 underwent moderate metabolism after a single oral dose of 50-mg (100 μCi) and was eliminated in humans via a combination of metabolism and excretion of unchanged parent. AG-881 metabolism involved the oxidation and conjugation with glutathione (GSH) by displacement of the chlorine at the chloropyridine moiety. Subsequent biotransformation of GSH intermediates resulted in elimination of both glutamic acid and glycine to form the cysteinyl conjugates (M515 and M499). The cysteinyl conjugates were further converted by a series of biotransformation reactions such as oxidation, S-dealkylation, S-methylation, S-oxidation, S-acetylation and N-dealkylation resulting in the formation multiple metabolites.
A summary of the metabolites observed is included in Table 2
Example 20
The spfash mutant mice were injected intravenously with a single dose of human OTC mRNA (either construct OTC-07 (SEQ ID NO:35) or OTC-12 (SEQ ID NO:40)) at either 0.5 mg/kg or 1.0 mg/kg, or a control mRNA encoding eGFP at a dose of 1 mg/kg, via tail vein injection. The mRNA was formulated in lipid nanoparticles (Compound II) for delivery into the mice. Urine was collected from mice 48 hours and 24 hours prior to mRNA injection for urinary orotic acid/creatinine analysis. All mice urine was collected for urinary orotic acid/creatinine levels 24 hours, 48 hours, 72 hours, 7 days, 14 days, or 21 days after dosing for each injected human OTC mRNA and for the injected eGFP control.
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More about "Urinalysis"
This process involves the physical, chemical, and microscopic analysis of urine samples to detect and monitor various health conditions, such as kidney disorders, urinary tract infections, and metabolic imbalances.
Urinalysis provides valuable insights into the body's overall well-being, helping clinicians make informed decisions about patient care.
By examining the color, clarity, pH, and specific gravity of urine, as well as the presence of various substances, urinalysis can help identify potential issues and guide appropriate treatment strategies.
This essential laboratory test is a vital component of comprehensive healthcare, contributing to the early detection and management of a wide range of medical conditions.
Urinalysis can be performed using a variety of tools and methods, including Multistix 10 SG, SAS 9.4, SAS version 9.4, Urine protein test kits, Cobas 6000, AU5800, Clinitek Status Analyzer, Combur 10 Test, Prism 8, and Clarus 600T.
These technologies and products offer advanced analysis capabilities, enabling healthcare professionals to accurately assess urine samples and make informed decisions about patient care.
The findings from urinalysis can also be used to monitor the effectiveness of treatment and track the progression or resolution of various medical conditions.
With the help of cutting-edge AI platforms like PubCompare.ai, researchers can optimize their urinalysis studies by identifying the most effective protocols and methods from literature, preprints, and patents, ensuring reproducibility and accuracy in their research.