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Molecular Biology Research Technique

Unlocking Biomolecular Insights: Essential Techniques for Cutting-Edge Molecular Biology Research

Alkaline Comet Assay
1 650 protocols
The Alkaline Comet Assay is a widely used technique in molecular biology and toxicology research for the detection and quantification of DNA damage at the single-cell level.
This sensitive and reliable assay is particularly valuable in evaluating genotoxicity, DNA repair mechanisms, and cellular responses to various environmental stressors or drug treatments.
The Alkaline Comet Assay has become a standard protocol for researchers investigating DNA integrity, oxidative stress, and genetic stability in a variety of cell types, making it a crucial tool for understanding fundamental cellular processes and the impact of different stimuli on genomic stability.
Amplified Fragment Length Polymorphism Analysis
548 protocols
Amplified Fragment Length Polymorphism (AFLP) Analysis is a versatile molecular technique that has become a valuable tool in various scientific investigations.
By leveraging the power of DNA fingerprinting, AFLP analysis allows researchers to explore genetic diversity, identify genetic markers, and conduct population genetics studies across a wide range of organisms, from plants and animals to microbes.
With its ability to generate a large number of highly informative and reproducible genetic markers, AFLP analysis has found extensive applications in fields such as plant breeding, microbial strain identification, and evolutionary biology, making it a crucial component in many research protocols.
Array-Based Comparative Genomic Hybridization
1 099 protocols
Array-Based Comparative Genomic Hybridization (Array-CGH) is a powerful genomic profiling technique that enables researchers to detect and analyze copy number variations (CNVs) across the entire genome.
This high-resolution method is widely used in a variety of scientific applications, including cancer research, genetic disease investigation, and developmental biology studies, providing valuable insights into the underlying genomic changes associated with these complex biological processes.
Array-CGH has become an indispensable tool for researchers seeking to unravel the genetic basis of disease and drive advancements in personalized medicine and targeted therapeutics.
ATAC-Seq
10 247 protocols
ATAC-Seq (Assay for Transposase-Accessible Chromatin using Sequencing) is a powerful technique that enables researchers to map open chromatin regions genome-wide.
This method provides insights into gene regulation and transcriptional dynamics, making it a valuable tool for epigenetic studies, cell-type identification, and the investigation of chromatin accessibility in various biological systems.
ATAC-Seq is widely used in a range of scientific experiments, from characterizing transcriptional regulatory networks to uncovering novel disease-associated genetic variants.
Biolistics
1 937 protocols
Biolistics, also known as gene gun technology, is a powerful tool in the field of molecular biology and genetics.
This technique utilizes high-velocity microparticles coated with genetic material to deliver DNA, RNA, or proteins directly into living cells, tissues, or organisms.
Biolistics has diverse applications, including gene expression studies, vaccine development, plant genetic engineering, and various other scientific experiments that require the efficient and targeted introduction of genetic material into samples.
Researchers investigating protocols involving Biolistics can leverage this technology to expand their research capabilities and uncover new insights in their areas of study.
Candidate Gene Analysis
3 888 protocols
Candidate Gene Analysis is a powerful approach in genetic research, allowing scientists to investigate the role of specific genes in various biological processes and disease states.
By targeting pre-selected candidate genes based on their known or suspected function, researchers can efficiently explore the genetic underpinnings of complex traits, identify disease-associated variants, and develop targeted therapeutic strategies.
This targeted approach has found widespread applications in areas such as population genetics, pharmacogenomics, and the study of complex disorders, making it a valuable tool in the arsenal of modern genetic research protocols.
Candidate Gene Identification
4 230 protocols
Candidate Gene Identification is a powerful research approach that plays a crucial role in modern scientific experiments.
By leveraging genomic and genetic data, this technique allows researchers to pinpoint specific genes that may be associated with a particular trait, disease, or biological process of interest.
This targeted approach is commonly applied in fields such as genetics, molecular biology, and bioinformatics, where it helps to streamline research, accelerate discovery, and uncover valuable insights that can drive scientific advancements.
ChIP-Chip
2 928 protocols
ChIP-Chip, or Chromatin Immunoprecipitation-microarray, is a powerful technique that allows researchers to study the interactions between proteins and DNA on a genome-wide scale.
This method combines the specificity of chromatin immunoprecipitation (ChIP) with the high-throughput capabilities of DNA microarray technology, enabling the identification of transcription factor binding sites, histone modifications, and other protein-DNA interactions.
Widely used in epigenetic research, ChIP-Chip is crucial for understanding gene regulation, developmental processes, and the role of chromatin structure in various biological systems.
ChIP-PET
719 protocols
ChIP-PET (Chromatin Immunoprecipitation-Paired End Tagging) is a powerful technique that combines chromatin immunoprecipitation (ChIP) with high-throughput sequencing to map the genome-wide binding sites of transcription factors and other DNA-binding proteins.
This technique is widely used in epigenetic research, gene regulation studies, and the identification of transcription factor-binding sites, providing valuable insights into the complex mechanisms that govern gene expression and cellular processes.
By incorporating relevant keywords like "ChIP-PET," "chromatin immunoprecipitation," "transcription factors," and "epigenetics," this paragraph can help researchers interested in this technique find relevant protocols and information.
Chromatin Immunoprecipitation Sequencing
29 016 protocols
Chromatin Immunoprecipitation Sequencing (ChIP-Seq) is a powerful technique used to study the interactions between DNA-binding proteins and specific genomic regions.
This protocol allows researchers to identify the genomic locations where transcription factors, epigenetic regulators, and other regulatory proteins bind, providing valuable insights into gene expression, chromatin organization, and transcriptional regulation.
ChIP-Seq has become an indispensable tool in fields such as epigenetics, developmental biology, and cancer research, enabling scientists to unravel the complex mechanisms underlying gene regulation and cellular processes.
Chromosome Mapping
13 081 protocols
Chromosome Mapping is a powerful technique used in various scientific experiments to study the genetic makeup of organisms.
This method involves identifying the physical location of genes on specific chromosomes, providing valuable insights into gene expression, inheritance patterns, and genome organization.
Chromosome Mapping is widely employed in fields such as genetics, genomics, and evolutionary biology, and is particularly relevant for applications like gene mapping, linkage analysis, and comparative genomics.
Comet Assay
10 530 protocols
The Comet Assay, also known as the single-cell gel electrophoresis (SCGE) assay, is a widely used technique for evaluating DNA damage and repair in individual cells.
This versatile tool has become an essential part of many research protocols, particularly in fields such as toxicology, genotoxicity testing, and environmental monitoring.
By providing a sensitive and quantitative assessment of DNA strand breaks, the Comet Assay allows researchers to gain valuable insights into the effects of various chemical, physical, or biological agents on cellular genetic integrity, making it a crucial component in a wide range of scientific experiments.
Comparative Genomic Hybridization
2 662 protocols
Comparative Genomic Hybridization (CGH) is a powerful genomic technique that allows researchers to analyze and compare the copy number variations (CNVs) between a test sample and a reference sample.
This method is widely used in various scientific disciplines, including cancer research, genetic disorder studies, and evolutionary biology, to identify genomic imbalances and chromosomal aberrations.
CGH protocols have become an essential tool for researchers seeking to unravel the complex genetic landscape of their experimental models and gain valuable insights into the underlying mechanisms driving biological processes.
Cross-Linking and Immunoprecipitation Followed by Deep Sequencing
944 protocols
Cross-Linking and Immunoprecipitation Followed by Deep Sequencing (CLIP-seq) is a powerful technique that enables researchers to study protein-RNA interactions on a genome-wide scale.
This method combines in vivo cross-linking, immunoprecipitation, and deep sequencing to identify the specific RNA regions bound by a protein of interest, providing invaluable insights into post-transcriptional regulatory mechanisms.
CLIP-seq has become a widely adopted approach in various fields, including transcriptomics, epigenetics, and molecular biology, allowing scientists to uncover the intricate relationships between RNA-binding proteins and their target transcripts.
Cytogenetic Analysis
2 513 protocols
Cytogenetic Analysis is a fundamental technique in scientific research, providing invaluable insights into the structure and behavior of chromosomes.
This powerful analytical method is widely employed across diverse fields, from investigating genetic disorders and cancer biology to understanding evolutionary processes and species identification.
By examining the chromosome complement of cells, researchers can uncover critical information about an organism's genomic composition, karyotype, and genetic alterations, making Cytogenetic Analysis an indispensable tool in the arsenal of modern scientific investigation.
DNA Fingerprinting
4 627 protocols
DNA Fingerprinting: A Powerful Genomic Tool for Scientific Exploration
DNA fingerprinting, a revolutionary genetic analysis technique, has become an indispensable tool in scientific research across various disciplines.
By leveraging the unique DNA sequences of individuals, this method enables researchers to accurately identify, compare, and analyze genetic profiles for a wide range of applications, from forensic investigations and paternity testing to wildlife conservation and evolutionary studies.
As a versatile and reliable protocol, DNA fingerprinting continues to drive scientific advancements, providing researchers with invaluable insights into the genetic makeup of organisms and the relationships between them.
Fluorescent in Situ Hybridization
14 541 protocols
Fluorescent in Situ Hybridization (FISH) is a powerful molecular biology technique that enables the visualization and localization of specific DNA or RNA sequences within cells or tissue samples.
This versatile method is widely employed in various scientific fields, including cytogenetics, cancer research, and microbiology, to detect and analyze chromosomal aberrations, gene expression patterns, and the presence of specific pathogens or genetic markers.
FISH protocols offer researchers a valuable tool for understanding the spatial organization and dynamics of genetic material, contributing to advancements in our understanding of cellular processes and the development of diagnostic and therapeutic applications.
Gene Annotation
36 321 protocols
Gene Annotation is a crucial process in bioinformatics and molecular biology research, where scientists identify and describe the functional elements within a genomic sequence.
This technique is particularly relevant for understanding gene structure, expression patterns, and potential biological roles, enabling researchers to design more targeted and effective experiments.
Common applications of Gene Annotation include genome assembly, transcriptome analysis, and the identification of novel genes or regulatory regions, which are essential for a wide range of scientific investigations, from disease research to evolutionary studies.
Gene Drive Systems
797 protocols
Gene Drive Systems are a revolutionary genetic engineering technology that allow for the targeted modification and propagation of specific genetic traits within a population.
This powerful tool has become increasingly relevant in scientific research, enabling researchers to address a wide range of applications, from the suppression of disease-carrying pests to the enhancement of beneficial organisms.
As a result, Gene Drive Systems have become a crucial part of many research protocols, providing scientists with a versatile and efficient approach to understanding and manipulating biological systems.
Gene Editing
2 274 protocols
Gene Editing: A Powerful Tool for Scientific Exploration
Gene editing, also known as genome editing, is a revolutionary technology that allows scientists to precisely modify DNA sequences.
This technique has become an invaluable tool in modern scientific research, enabling researchers to study gene function, model disease, and develop novel therapeutic approaches.
From CRISPR-Cas9 to other advanced gene editing tools, these cutting-edge methodologies are widely used across various fields, including cell biology, genetics, and biotechnology, to unlock new frontiers in scientific discovery.
Gene Expression Microarray Analysis
1 228 protocols
Gene Expression Microarray Analysis is a powerful technique used in molecular biology and genetics research to study the expression levels of thousands of genes simultaneously.
By hybridizing labeled RNA or cDNA samples to high-density microarray chips, researchers can gain valuable insights into the transcriptional profile of cells, tissues, or organisms under different experimental conditions, such as disease states, drug treatments, or developmental stages.
This versatile approach has numerous applications, including biomarker discovery, disease diagnosis, drug target identification, and the elucidation of complex biological pathways and networks.
Gene Fusion
6 430 protocols
Gene Fusion is a powerful molecular biology technique that has gained significant prominence in scientific research.
By fusing two or more genes, researchers can create hybrid proteins with novel functionalities, enabling a wide range of applications, from the study of protein-protein interactions to the development of targeted therapeutic agents.
This versatile technique is widely employed in areas such as protein engineering, biomarker discovery, and the investigation of signal transduction pathways, making it a valuable tool for researchers across various disciplines.
Gene Knockdown Techniques
5 958 protocols
Gene Knockdown Techniques are powerful molecular biology tools that enable the selective silencing or downregulation of target genes.
These techniques, such as RNA interference (RNAi) and CRISPR-Cas9 systems, are widely used in scientific research to study gene function, model disease pathways, and develop novel therapeutic approaches.
Researchers often employ Gene Knockdown Techniques to investigate the roles of specific genes in cellular processes, pathological conditions, and to validate potential drug targets, making them a crucial component of experimental design and protocols in various fields of study.
Gene Knockout Techniques
6 461 protocols
Gene Knockout Techniques are a powerful set of genetic engineering tools that allow researchers to selectively inactivate or "knock out" specific genes in cells or organisms.
These techniques are widely used in scientific research to study gene function, model human diseases, and develop potential therapeutic interventions.
By systematically disrupting individual genes, researchers can gain valuable insights into the roles of different genes in biological processes, paving the way for advancements in fields such as molecular biology, genetics, and drug discovery.
Genetic Association Studies
2 002 protocols
Genetic Association Studies (GAS) are a powerful tool in the field of genomic research, providing valuable insights into the genetic underpinnings of complex traits and diseases.
By examining the association between genetic variants and specific phenotypes, GAS studies can help identify genetic risk factors, unveil novel disease pathways, and guide the development of targeted therapies.
Widely used in a range of scientific experiments, from population-based studies to genome-wide association studies (GWAS), GAS protocols have become an essential component of modern genetic research, contributing to advancements in areas such as personalized medicine, pharmacogenomics, and complex trait analysis.
Genetic Complementation Test
2 061 protocols
The Genetic Complementation Test is a powerful tool used in scientific research to determine whether two mutations or genetic defects are located within the same gene or different genes.
By assessing the ability of mutant genes to complement each other and restore normal function, researchers can gain valuable insights into the genetic basis of various biological processes and disorders.
This test is widely employed in fields such as molecular biology, genetics, and developmental biology, providing researchers with a reliable method to uncover gene interactions and elucidate gene function.
Genetic Engineering
1 993 protocols
Genetic engineering, the process of manipulating an organism's genetic material, has become a cornerstone of modern scientific research.
This versatile technique allows researchers to introduce, modify, or remove specific genes, enabling a wide range of applications, from developing new therapies and improving crop yields to studying gene function and modeling genetic diseases.
As a foundational approach in many fields, including molecular biology, biotechnology, and medicine, genetic engineering remains a crucial tool for advancing scientific knowledge and addressing real-world challenges.
Genome-Wide Association Study
51 102 protocols
Genome-Wide Association Study (GWAS) is a powerful research protocol that examines the association between genetic variations across the entire genome and specific traits, diseases, or phenotypes.
This high-throughput approach has become increasingly important in modern genetic and genomic research, enabling scientists to identify genetic markers and potential causal variants associated with complex traits and diseases.
GWAS has been widely applied in various fields, including human health, agriculture, and evolutionary biology, providing valuable insights into the genetic architecture of complex phenotypes and informing future targeted studies and therapeutic interventions.
Genotyping Techniques
3 883 protocols
Genotyping Techniques are a crucial component of modern scientific research, offering researchers powerful tools to investigate genetic profiles and uncover valuable insights.
This powerful analytical approach allows scientists to explore genetic variations, identify genetic markers, and characterize complex traits, making it a cornerstone of diverse applications, from personalized medicine and population genetics to evolutionary studies and forensic analysis.
Incorporating Genotyping Techniques into your research protocol can unlock a wealth of genetic data, empowering you to make informed decisions, drive scientific discoveries, and advance your field of study.
Illumina Sequencing
854 protocols
Illumina Sequencing is a powerful DNA sequencing technology that has revolutionized genomic research across various disciplines.
This next-generation sequencing (NGS) platform enables researchers to generate high-quality, high-throughput genetic data with unparalleled efficiency, making it a go-to choice for a wide range of applications, including genome assembly, transcriptome analysis, epigenetic profiling, and targeted sequencing of specific genomic regions.
Illumina Sequencing has become an indispensable tool in the scientific community, providing researchers with unprecedented insights into the genetic makeup of organisms, ultimately driving advancements in fields such as biology, medicine, and evolutionary studies.